NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|480339775|gb|ENX59966|]
View 

hypothetical protein F885_02158 [Acinetobacter higginsii]

Protein Classification

AraC family transcriptional regulator( domain architecture ID 11677401)

AraC family transcriptional regulator controls the expression of genes with diverse biological functions including metabolism, stress response, and virulence

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
HTH_18 pfam12833
Helix-turn-helix domain;
263-338 3.67e-16

Helix-turn-helix domain;


:

Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 72.24  E-value: 3.67e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 480339775  263 ALDIPERTLRHQLQQ-MQTSYKEIRERLIKQKAIKF-IENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQYNRN 338
Cdd:pfam12833   4 ALGMSPRTLSRLFKReLGLSPKEYLRRLRLERARRLlLEDTGLSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRRR 81
Arabinose_bd super family cl14988
Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial ...
 19-197 4.18e-13

Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial transcriptional regulatory protein with a DNA-binding domain at the C-terminus, HTH_AraC, pfam00165, and this dimerization domain which harbours the arabinose-binding pocket at the N-terminus. AraC positively and negatively regulates expression of the proteins required for the uptake and catabolism of the sugar L-arabinose 1,2,3].


The actual alignment was detected with superfamily member pfam12625:

Pssm-ID: 432679  Cd Length: 185  Bit Score: 66.90  E-value: 4.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   19 GIDVDARLANMGIQADAL-DPSSIIPDEIE---WDILQHISQDisPEQGLFIGQHYALAGYGPFLMLLVTSDTLHKALLN 94
Cdd:pfam12625   1 GLDPAALLRRAGLDPALLaDPDARVPLDQYlrlWEAAARELGD--PALGLRLGRRLRPGSLGLLGYAALSSPTLGEALDR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   95 GVQYQQLTHLFGSLQLKI-EDDLIYLLYRPIDLSMHLGQLRAQCEISGTYKFLQDifkMMGLEVPQIQIELPFHRPKDlt 173
Cdd:pfam12625  79 LLRYLRLLSDALELRLEEgEGGLARLRLDVRPIPGRRRDFLLELALAALLRLARW---LLGRRLPPLRVEFPHPAPAD-- 153

                         170       180
                  ....*....|....*....|....
gi 480339775  174 lLAAYQDYYGQDVKFGCAQAAFIL 197
Cdd:pfam12625 154 -AAEYERLFGCPVRFGAPRNALVF 176
 
Name Accession Description Interval E-value
HTH_18 pfam12833
Helix-turn-helix domain;
263-338 3.67e-16

Helix-turn-helix domain;


Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 72.24  E-value: 3.67e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 480339775  263 ALDIPERTLRHQLQQ-MQTSYKEIRERLIKQKAIKF-IENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQYNRN 338
Cdd:pfam12833   4 ALGMSPRTLSRLFKReLGLSPKEYLRRLRLERARRLlLEDTGLSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRRR 81
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
255-335 4.07e-16

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 72.20  E-value: 4.07e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   255 PSMAETACALDIPERTLRHQLQQ-MQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPR 333
Cdd:smart00342   2 LTLEDLAEALGVSPRHLQRLFKKeTGTTPKQYLRDRRLERARRLLRDTDLSVTEIALRVGFSSQSYFSRAFKKLFGVTPS 81


                   ..
gi 480339775   334 QY 335
Cdd:smart00342  82 EY 83
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
256-339 3.26e-15

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 74.43  E-value: 3.26e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 256 SMAETACALDIPERTLRHQL-QQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQ 334
Cdd:COG2207  170 TLEELARELGLSPRTLSRLFkEETGTSPKQYLRELRLERAKRLLAETDLSISEIAYELGFSSQSHFSRAFKKRFGVTPSE 249


                 ....*
gi 480339775 335 YNRNH 339
Cdd:COG2207  250 YRKRL 254
Arabinose_bd pfam12625
Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial ...
 19-197 4.18e-13

Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial transcriptional regulatory protein with a DNA-binding domain at the C-terminus, HTH_AraC, pfam00165, and this dimerization domain which harbours the arabinose-binding pocket at the N-terminus. AraC positively and negatively regulates expression of the proteins required for the uptake and catabolism of the sugar L-arabinose 1,2,3].


Pssm-ID: 432679  Cd Length: 185  Bit Score: 66.90  E-value: 4.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   19 GIDVDARLANMGIQADAL-DPSSIIPDEIE---WDILQHISQDisPEQGLFIGQHYALAGYGPFLMLLVTSDTLHKALLN 94
Cdd:pfam12625   1 GLDPAALLRRAGLDPALLaDPDARVPLDQYlrlWEAAARELGD--PALGLRLGRRLRPGSLGLLGYAALSSPTLGEALDR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   95 GVQYQQLTHLFGSLQLKI-EDDLIYLLYRPIDLSMHLGQLRAQCEISGTYKFLQDifkMMGLEVPQIQIELPFHRPKDlt 173
Cdd:pfam12625  79 LLRYLRLLSDALELRLEEgEGGLARLRLDVRPIPGRRRDFLLELALAALLRLARW---LLGRRLPPLRVEFPHPAPAD-- 153

                         170       180
                  ....*....|....*....|....
gi 480339775  174 lLAAYQDYYGQDVKFGCAQAAFIL 197
Cdd:pfam12625 154 -AAEYERLFGCPVRFGAPRNALVF 176
ftrA PRK09393
transcriptional activator FtrA; Provisional
 254-338 1.24e-08

transcriptional activator FtrA; Provisional


Pssm-ID: 181818 [Multi-domain]  Cd Length: 322  Bit Score: 55.74  E-value: 1.24e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 254 IPSMAETACaldIPERT-LRHQLQQMQTSYKE--IRERLIKQKAIkfIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGL 330
Cdd:PRK09393 237 VASLAARAA---MSPRTfLRRFEAATGMTPAEwlLRERLARARDL--LESSALSIDQIAERAGFGSEESLRHHFRRRAAT 311


                 ....*...
gi 480339775 331 SPRQYNRN 338
Cdd:PRK09393 312 SPAAYRKR 319
 
Name Accession Description Interval E-value
HTH_18 pfam12833
Helix-turn-helix domain;
263-338 3.67e-16

Helix-turn-helix domain;


Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 72.24  E-value: 3.67e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 480339775  263 ALDIPERTLRHQLQQ-MQTSYKEIRERLIKQKAIKF-IENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQYNRN 338
Cdd:pfam12833   4 ALGMSPRTLSRLFKReLGLSPKEYLRRLRLERARRLlLEDTGLSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRRR 81
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
255-335 4.07e-16

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 72.20  E-value: 4.07e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   255 PSMAETACALDIPERTLRHQLQQ-MQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPR 333
Cdd:smart00342   2 LTLEDLAEALGVSPRHLQRLFKKeTGTTPKQYLRDRRLERARRLLRDTDLSVTEIALRVGFSSQSYFSRAFKKLFGVTPS 81


                   ..
gi 480339775   334 QY 335
Cdd:smart00342  82 EY 83
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
256-339 3.26e-15

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 74.43  E-value: 3.26e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 256 SMAETACALDIPERTLRHQL-QQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQ 334
Cdd:COG2207  170 TLEELARELGLSPRTLSRLFkEETGTSPKQYLRELRLERAKRLLAETDLSISEIAYELGFSSQSHFSRAFKKRFGVTPSE 249


                 ....*
gi 480339775 335 YNRNH 339
Cdd:COG2207  250 YRKRL 254
Arabinose_bd pfam12625
Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial ...
 19-197 4.18e-13

Arabinose-binding domain of AraC transcription regulator, N-term; AraC is a bacterial transcriptional regulatory protein with a DNA-binding domain at the C-terminus, HTH_AraC, pfam00165, and this dimerization domain which harbours the arabinose-binding pocket at the N-terminus. AraC positively and negatively regulates expression of the proteins required for the uptake and catabolism of the sugar L-arabinose 1,2,3].


Pssm-ID: 432679  Cd Length: 185  Bit Score: 66.90  E-value: 4.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   19 GIDVDARLANMGIQADAL-DPSSIIPDEIE---WDILQHISQDisPEQGLFIGQHYALAGYGPFLMLLVTSDTLHKALLN 94
Cdd:pfam12625   1 GLDPAALLRRAGLDPALLaDPDARVPLDQYlrlWEAAARELGD--PALGLRLGRRLRPGSLGLLGYAALSSPTLGEALDR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775   95 GVQYQQLTHLFGSLQLKI-EDDLIYLLYRPIDLSMHLGQLRAQCEISGTYKFLQDifkMMGLEVPQIQIELPFHRPKDlt 173
Cdd:pfam12625  79 LLRYLRLLSDALELRLEEgEGGLARLRLDVRPIPGRRRDFLLELALAALLRLARW---LLGRRLPPLRVEFPHPAPAD-- 153

                         170       180
                  ....*....|....*....|....
gi 480339775  174 lLAAYQDYYGQDVKFGCAQAAFIL 197
Cdd:pfam12625 154 -AAEYERLFGCPVRFGAPRNALVF 176
GlxA COG4977
Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH ...
 239-339 2.65e-10

Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH domain [Transcription];


Pssm-ID: 444002 [Multi-domain]  Cd Length: 318  Bit Score: 60.56  E-value: 2.65e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 239 VVEYVEDYLEMQNGViPSMAETACaldIPERTL-RHQLQQMQTSYKE--IRERLikQKAIKFIENSSYSIEQVAEMLGYS 315
Cdd:COG4977  215 AQAWMEANLEEPLSV-DELARRAG---MSPRTLeRRFRAATGTTPARylQRLRL--ERARRLLETTDLSIEEIAAACGFG 288

                         90       100
                 ....*....|....*....|....
gi 480339775 316 EPAAFNHAFKRWFGLSPRQYNRNH 339
Cdd:COG4977  289 SASHFRRAFRRRFGVSPSAYRRRF 312
ftrA PRK09393
transcriptional activator FtrA; Provisional
 254-338 1.24e-08

transcriptional activator FtrA; Provisional


Pssm-ID: 181818 [Multi-domain]  Cd Length: 322  Bit Score: 55.74  E-value: 1.24e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 254 IPSMAETACaldIPERT-LRHQLQQMQTSYKE--IRERLIKQKAIkfIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGL 330
Cdd:PRK09393 237 VASLAARAA---MSPRTfLRRFEAATGMTPAEwlLRERLARARDL--LESSALSIDQIAERAGFGSEESLRHHFRRRAAT 311


                 ....*...
gi 480339775 331 SPRQYNRN 338
Cdd:PRK09393 312 SPAAYRKR 319
PRK15186 PRK15186
AraC family transcriptional regulator; Provisional
 270-335 6.38e-07

AraC family transcriptional regulator; Provisional


Pssm-ID: 185108 [Multi-domain]  Cd Length: 291  Bit Score: 50.06  E-value: 6.38e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 480339775 270 TLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQY 335
Cdd:PRK15186 213 TLKRKLKQENTSFSEVYLNARMNKATKLLRNSEYNITRVAYMCGYDSASYFTCVFKKHFKTTPSEF 278
PRK09978 PRK09978
DNA-binding transcriptional regulator GadX; Provisional
 256-335 2.51e-06

DNA-binding transcriptional regulator GadX; Provisional


Pssm-ID: 137624 [Multi-domain]  Cd Length: 274  Bit Score: 48.38  E-value: 2.51e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 256 SMAETACALDIPERTLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQY 335
Cdd:PRK09978 160 TLARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEY 239
HTH_AraC pfam00165
Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the ...
 295-335 1.92e-05

Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the N-terminal arm of AraC binds to the DNA binding domain (pfam00165) and helps to hold the two DNA binding domains in a relative orientation that favours DNA looping. In the presence of arabinose, the arms bind over the arabinose on the dimerization domain, thus freeing the DNA-binding domains. The freed DNA-binding domains are then able to assume a conformation suitable for binding to the adjacent DNA sites that are utilized when AraC activates transcription, and hence AraC ceases looping the DNA when arabinose is added.


Pssm-ID: 425497 [Multi-domain]  Cd Length: 42  Bit Score: 41.37  E-value: 1.92e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 480339775  295 IKFIENSSYSIEQVAEMLGYSePAAFNHAFKRWFGLSPRQY 335
Cdd:pfam00165   1 LRENLSTNLTIADIADELGFS-RSYFSRLFKKYTGVTPSQY 40
PRK15044 PRK15044
transcriptional regulator SirC; Provisional
 256-339 3.82e-05

transcriptional regulator SirC; Provisional


Pssm-ID: 185004 [Multi-domain]  Cd Length: 295  Bit Score: 44.64  E-value: 3.82e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 256 SMAETACALDIPERTLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQY 335
Cdd:PRK15044 210 SQAEVAGKLFMSVSSLKRKLAAEEVSFSKIYLDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSF 289


                 ....*.
gi 480339775 336 NR--NH 339
Cdd:PRK15044 290 MRtmNH 295
PRK09940 PRK09940
transcriptional regulator YdeO; Provisional
 264-339 1.06e-04

transcriptional regulator YdeO; Provisional


Pssm-ID: 182157 [Multi-domain]  Cd Length: 253  Bit Score: 43.15  E-value: 1.06e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 480339775 264 LDIPERTLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSySIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQYNRNH 339
Cdd:PRK09940 160 LYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEG-SVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEY 234
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
263-335 2.26e-04

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 42.07  E-value: 2.26e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 480339775 263 ALDIPERTLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLGYSePAAFNHAFKRWFGLSPRQY 335
Cdd:COG2207  129 ALLRALELLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLTLEELARELGLS-PRTLSRLFKEETGTSPKQY 200
PRK15185 PRK15185
transcriptional regulator HilD; Provisional
 234-339 8.70e-04

transcriptional regulator HilD; Provisional


Pssm-ID: 185107 [Multi-domain]  Cd Length: 309  Bit Score: 40.75  E-value: 8.70e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 234 SLKLTVVEYVEDYLEMQNGVIPSMAETACALDIPERTLRHQLQQMQTSYKEIRERLIKQKAIKFIENSSYSIEQVAEMLG 313
Cdd:PRK15185 202 SAQITLKERVYNIISSSPSRQWKLTDVADHIFMSTSTLKRKLAEEGTSFSDIYLSARMNQAAKLLRIGNHNVNAVALKCG 281

                         90       100
                 ....*....|....*....|....*...
gi 480339775 314 YSEPAAFNHAFKRWFGLSPRQYNR--NH 339
Cdd:PRK15185 282 YDSTSYFIQCFKKYFKTTPSTFIKmaNH 309
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 255-339 9.36e-03

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 37.34  E-value: 9.36e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480339775 255 PSMAETACALDIPERTLRHQL-QQMQTSYKE----IRERLIKQkAIKfienSSYSIEQVAEMLGYSEPAAFNHAFKRWFG 329
Cdd:COG2169  101 PSLEDLAARLGLSPRHLRRLFkAHTGVTPKAyaraRRLLRARQ-LLQ----TGLSVTDAAYAAGFGSLSRFYEAFKKLLG 175

                         90
                 ....*....|
gi 480339775 330 LSPRQYNRNH 339
Cdd:COG2169  176 MTPSAYRRGG 185
PRK10572 PRK10572
arabinose operon transcriptional regulator AraC;
276-339 9.46e-03

arabinose operon transcriptional regulator AraC;


Pssm-ID: 236717 [Multi-domain]  Cd Length: 290  Bit Score: 37.26  E-value: 9.46e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 480339775 276 QQMQTSYKEIRE--RLIKQKaiKFIENSSYSIEQVAEMLGYSEPAAFNHAFKRWFGLSPRQYNRNH 339
Cdd:PRK10572 222 QQLGISVLRWREdqRISRAK--LLLQTTRMPIATIGRNVGYDDQLYFSRVFKKCTGASPSEFRARC 285
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH