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Conserved domains on  [gi|480892069|gb|ENZ75289|]
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succinylglutamate desuccinylase [Ralstonia pickettii OR214]

Protein Classification

M14 family metallopeptidase( domain architecture ID 10161028)

M14 family metallopeptidase is a zinc-binding carboxypeptidase which hydrolyzes a single, C-terminal amino acid from a polypeptide chain, and has a recognition site for the free C-terminal carboxyl group

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 21-228 2.61e-138

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


:

Pssm-ID: 349481  Cd Length: 208  Bit Score: 390.17  E-value: 2.61e-138
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  21 DIRRWKTGTHGIDYVHTSDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 100
Cdd:cd06910    1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 101 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 180
Cdd:cd06910   81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 480892069 181 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 228
Cdd:cd06910  161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
 
Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 21-228 2.61e-138

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 390.17  E-value: 2.61e-138
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  21 DIRRWKTGTHGIDYVHTSDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 100
Cdd:cd06910    1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 101 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 180
Cdd:cd06910   81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 480892069 181 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 228
Cdd:cd06910  161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
COG3608 COG3608
Predicted deacylase [General function prediction only];
35-321 1.47e-34

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 127.66  E-value: 1.47e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  35 VHTSDSGVPGPHVMINALTHGNELCGAIAVDALLAAgVRPA--RGRLTL-SFANVEAYERFdindpdaTRFI---DEDLN 108
Cdd:COG3608   17 VTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRE-LDPGelRGTVILvPVANPPGFLQG-------SRYLpidGRDLN 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 109 RVWSADKlDGpgnSLELRRAREL-RPVIDTVDYLLDIHSMHEKAE--PLMLTGP-HAKGVALGRLVGAPshLMIDAGHAA 184
Cdd:COG3608   89 RSFPGDA-DG---SLAERIAHALfEEILPDADYVIDLHSGGIARDnlPHVRAGPgDEELRALARAFGAP--VILDSPEGG 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 185 GRRLRDYGgfgdAASPKNALLIECGQH--FERVSRDVALDACARFLVALGTVDASAVSewlgmAPALEVRCIRVTDPVVA 262
Cdd:COG3608  163 DGSLREAA----AEAGIPALTLELGGGgrFDEESIEAGVRGILNVLRHLGMLDGEAPP-----PPLAPPVLARGSEWVRA 233

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 480892069 263 KSTA-FRFaddYRGMETIARAGTVIAT------DGALQIVTPYDNCVLLQPSLRHLAPGVTVVRLG 321
Cdd:COG3608  234 PAGGlFEP---LVELGDRVKKGDVLGRitdpfgEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
PRK02259 PRK02259
aspartoacylase; Provisional
 47-170 7.23e-10

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 58.73  E-value: 7.23e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPGNS-L 123
Cdd:PRK02259   5 VAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTviGNPEAIEA-------GRRYIDRDLNRSFRLDLLQNPDLSgY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 480892069 124 ELRRARELRPVI-----DTVDYLLDIHS----MhekAEPLMLTGPHAKGVALGRLV 170
Cdd:PRK02259  78 EQLRAKELVQQLgpkgnSPCDFIIDLHSttanM---GLSLILYGRRPFDLALAAYL 130
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 43-146 7.31e-10

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 58.90  E-value: 7.31e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069   43 PGPHVMINALTHGNELCGAIAVDAL---LAAGVRPARgRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP 119
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLlrqLDPGDIAGE-RTLVPLANPPAFRA-------GSRYIPRDLNRSFPGRALGAS 72

                          90       100       110
                  ....*....|....*....|....*....|...
gi 480892069  120 G-NSLELRRAREL-----RPVIDTVDYLLDIHS 146
Cdd:pfam04952  73 SdEPYRATRAERLadlffPALLPRADIVLDLHT 105
 
Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 21-228 2.61e-138

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 390.17  E-value: 2.61e-138
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  21 DIRRWKTGTHGIDYVHTSDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 100
Cdd:cd06910    1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 101 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 180
Cdd:cd06910   81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 480892069 181 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 228
Cdd:cd06910  161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
COG3608 COG3608
Predicted deacylase [General function prediction only];
35-321 1.47e-34

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 127.66  E-value: 1.47e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  35 VHTSDSGVPGPHVMINALTHGNELCGAIAVDALLAAgVRPA--RGRLTL-SFANVEAYERFdindpdaTRFI---DEDLN 108
Cdd:COG3608   17 VTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRE-LDPGelRGTVILvPVANPPGFLQG-------SRYLpidGRDLN 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 109 RVWSADKlDGpgnSLELRRAREL-RPVIDTVDYLLDIHSMHEKAE--PLMLTGP-HAKGVALGRLVGAPshLMIDAGHAA 184
Cdd:COG3608   89 RSFPGDA-DG---SLAERIAHALfEEILPDADYVIDLHSGGIARDnlPHVRAGPgDEELRALARAFGAP--VILDSPEGG 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 185 GRRLRDYGgfgdAASPKNALLIECGQH--FERVSRDVALDACARFLVALGTVDASAVSewlgmAPALEVRCIRVTDPVVA 262
Cdd:COG3608  163 DGSLREAA----AEAGIPALTLELGGGgrFDEESIEAGVRGILNVLRHLGMLDGEAPP-----PPLAPPVLARGSEWVRA 233

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 480892069 263 KSTA-FRFaddYRGMETIARAGTVIAT------DGALQIVTPYDNCVLLQPSLRHLAPGVTVVRLG 321
Cdd:COG3608  234 PAGGlFEP---LVELGDRVKKGDVLGRitdpfgEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 47-227 2.82e-18

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 80.57  E-value: 2.82e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNELCGAIAVDALLAAGVRPA--RGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKldgPGNSLE 124
Cdd:cd18430    1 LAVLGAVHGNETCGTRAVERLLAELPSGAlqKGPVTLVPANERAYAE-------GVRFCEEDLNRVFPGDP---DPDTYE 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 125 LRRARELRPVIDTVDYLLDIHSMHEKAEPL-MLTGPHAKGVALGRLVGAPSHL-MIDaghaaGRRLRDYGGFGdaASPKN 202
Cdd:cd18430   71 RRLANRLCPELEGHDVVLDLHSTHSGGQPFaILDYGDKASRRLARSVGIPKGWrVVY-----GRDLGYAHGGG--KTEVT 143

                        170       180
                 ....*....|....*....|....*
gi 480892069 203 ALLIECGQHFERVSRDVALDACARF 227
Cdd:cd18430  144 GVTVECGYHDSEEAAEVAYRAILNT 168
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 47-146 1.49e-15

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349480  Cd Length: 190  Bit Score: 73.78  E-value: 1.49e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP--GNS 122
Cdd:cd06909    3 VAIVGGTHGNELTGVYLVKHWLKNPELIERKSFEVHPllANPRAVEQ-------CRRYIDTDLNRCFSLENLSSApsSLP 75

                         90       100
                 ....*....|....*....|....*....
gi 480892069 123 LELRRAREL----RPVIDT-VDYLLDIHS 146
Cdd:cd06909   76 YEVRRAREInqilGPKGNPaCDFIIDLHN 104
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
44-146 8.08e-15

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 73.73  E-value: 8.08e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  44 GPHVMINALTHGNELCGAIAVDAL---LAAGVRPARGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPG 120
Cdd:COG2988   24 IKAVVISGGIHGNETAPIELLDKLlqdLLLGERPLSFRLLLILGNPAAMRA-------GRRYLDEDLNRLFGGRHLQNPE 96

                         90       100       110
                 ....*....|....*....|....*....|...
gi 480892069 121 NsLELRRARELRPVID-------TVDYLLDIHS 146
Cdd:COG2988   97 S-YEAARAKELEQAVGpffaaggRVRLHIDLHT 128
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 47-225 1.11e-14

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 70.80  E-value: 1.11e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNELCGAIAVDALLAAGVRPA-RGRLTL-SFANVEAYERfdindpdATRFIDE---DLNRVWSADkldgPGN 121
Cdd:cd06230    1 LLILAGVHGDEYEGVEAIRRLLAELDPSElKGTVVLvPVANPPAFEA-------GTRYTPLdglDLNRIFPGD----PDG 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 122 SLELRRARELRP-VIDTVDYLLDIHSMHEKAEP--LMLTGPHAKG---VALGRLVGAPSHLMIDaGHAAGrrlRDYGGFG 195
Cdd:cd06230   70 SPTERLAHELTElILKHADALIDLHSGGTGRLVpyAILDYDSDAReksRELARAFGGTPVIWGG-DPPGG---TPVAAAR 145

                        170       180       190
                 ....*....|....*....|....*....|
gi 480892069 196 DAASPknALLIECGQHFERVSRDVALDACA 225
Cdd:cd06230  146 SAGIP--AITVELGGGGRLRAERLERYLRG 173
PRK02259 PRK02259
aspartoacylase; Provisional
 47-170 7.23e-10

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 58.73  E-value: 7.23e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPGNS-L 123
Cdd:PRK02259   5 VAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTviGNPEAIEA-------GRRYIDRDLNRSFRLDLLQNPDLSgY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 480892069 124 ELRRARELRPVI-----DTVDYLLDIHS----MhekAEPLMLTGPHAKGVALGRLV 170
Cdd:PRK02259  78 EQLRAKELVQQLgpkgnSPCDFIIDLHSttanM---GLSLILYGRRPFDLALAAYL 130
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 43-146 7.31e-10

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 58.90  E-value: 7.31e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069   43 PGPHVMINALTHGNELCGAIAVDAL---LAAGVRPARgRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP 119
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLlrqLDPGDIAGE-RTLVPLANPPAFRA-------GSRYIPRDLNRSFPGRALGAS 72

                          90       100       110
                  ....*....|....*....|....*....|...
gi 480892069  120 G-NSLELRRAREL-----RPVIDTVDYLLDIHS 146
Cdd:pfam04952  73 SdEPYRATRAERLadlffPALLPRADIVLDLHT 105
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 47-150 1.19e-07

succinylglutamate desuccinylase; Provisional


Pssm-ID: 235408  Cd Length: 329  Bit Score: 52.49  E-value: 1.19e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  47 VMINALTHGNE-----LCGAIAVDalLAAGVRPARGRLTLSFANVEAyerfdINDpdATRFIDEDLNRVWSADKLDGPGn 121
Cdd:PRK05324  50 LVLSAGIHGNEtapieLLDQLVRD--LLAGELPLRARLLVILGNPPA-----MRA--GKRYLDEDLNRLFGGRHQQFPG- 119

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 480892069 122 SLELRRARELRPVIDT--------VDYLLDIH-----SMHEK 150
Cdd:PRK05324 120 SDEARRAAELEQAVEDffaagaerVRWHYDLHtairgSKHEQ 161
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 43-232 2.33e-06

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 47.53  E-value: 2.33e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  43 PGPHVMINALTHGNELCGaIAVDALLAAGVRPA--RGRLT-LSFANVEAYERFDINDPDATRfideDLNRVWsadkLDGP 119
Cdd:cd06251   11 PGPTLLLTAAIHGDELNG-IEVIQRLLEDLDPSklRGTLIaIPVVNPLGFENNSRYLPDDGR----DLNRSF----PGSE 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 120 GNSLELRRARELRP-VIDTVDYLLDIH--SMHEKAEPLML-TGPHAKGVALGRLVGAPshlMIDagHAAGRRlrdyGGFG 195
Cdd:cd06251   82 KGSLASRLAHLLWNeIVKKADYVIDLHtaSTGRTNLPYVRaDLRDPESRRMAEAFGAP---VIV--DDPGED----GSLR 152

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 480892069 196 DAASPKN--ALLIECGQH--FERVSRDVALDACARFLVALG 232
Cdd:cd06251  153 GAAVELGipAITVELGEAlrFDEDIIRRGVEGVLNVLRHLG 193
M14_ASTE cd03855
Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 ...
 54-150 2.51e-05

Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 Succinylglutamate desuccinylase (ASTE, also known as N-succinyl-L-glutamate amidohydrolase, N2-succinylglutamate desuccinylase, and SGDS; EC 3.5.1.96) belongs to the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily of the M14 family of metallocarboxypeptidases. This group includes succinylglutamate desuccinylase that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. It hydrolyzes N-succinyl-L-glutamate to succinate and L-glutamate.


Pssm-ID: 349428  Cd Length: 239  Bit Score: 44.89  E-value: 2.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  54 HGNE-----LCGAIAVDalLAAGVRPARGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPgNSLELRRA 128
Cdd:cd03855   53 HGNEtapieILDQLIND--LIRGELALAHRLLFIFGNPPAIRQ-------GKRFIEENLNRLFSGRHSKLP-PSYETARA 122

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 480892069 129 RELRPVID--------TVDYLLDIH-----SMHEK 150
Cdd:cd03855  123 AELEQAVAdffakasgEVRWHLDLHtairgSKHEQ 157
M14_ASTE_ASPA-like cd06254
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 42-153 5.48e-05

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349472  Cd Length: 198  Bit Score: 43.34  E-value: 5.48e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  42 VPGPHVMINALTHGNELCGAIAVdALLAAGVRPAR--GRLTL-SFANVEAYE--RFDINdpdatrFIDED-LNRVWSADK 115
Cdd:cd06254    9 KPGPTLLITAGIHGGEYPGILAA-IRLARELDPADvkGTLIIvHIANVSGFEarTPFVV------PEDGKnLNRVFPGDP 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 480892069 116 LDGPGNSLELRRARElrpVIDTVDYLLDIHS--MHEKAEP 153
Cdd:cd06254   82 DGTLTERIAYFLTRE---IISRADFLIDLHGgdANEALTP 118
M14_ASTE_ASPA-like cd06256
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 44-146 1.86e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349474  Cd Length: 204  Bit Score: 41.90  E-value: 1.86e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  44 GPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERfdindpdATRFID--EDLNRVWSadkldGPGN 121
Cdd:cd06256   34 PRPLFVSTLLHGNEPTGLRAVQRLLKTGQAPLPRTLLLFIGNVDAAKA-------GVRRLPgqPDYNRCWP-----GPFE 101

                         90       100
                 ....*....|....*....|....*..
gi 480892069 122 SLELRRARELRPVIDT--VDYLLDIHS 146
Cdd:cd06256  102 TPEGRLAAAVLERLDTlrPFASIDIHN 128
M14_ASTE_ASPA-like cd06255
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 43-233 2.72e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349473  Cd Length: 223  Bit Score: 41.54  E-value: 2.72e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  43 PGPHVMINALTHGNELCGAIAVDALLaAGVRPARGRLTLSF---AN---VEAYERFdindpdaTRFIDEDLNRVWSADKl 116
Cdd:cd06255   22 PGPCLWINGAVHGDELNGPLAALELF-RELDPAQLSGTLVAtpiANplaFQGRQKF-------SPQDGEDLDQSFPGDP- 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 117 DGPGNSlelRRA----RELRPVidtVDYLLDIHSMH----EKAEPLMLTGPHAKGVA------LGRLVGAPSHLMIDAGH 182
Cdd:cd06255   93 DGLITE---RMAhalfSEVKEV---ADYLIDFHTGGtpfdANPYTVYKLFPESGPVEekrllrLARAFGVHANCRVDVSG 166

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 480892069 183 AAGRRLRDYGGFGD---AASPKNALLIECG--QHFERVSRDVALDACARFLVALGT 233
Cdd:cd06255  167 AGGELPGNTAGALDyqcMAQGIPAFMVELGggGRAEEEAVRFAARGLRNLLRYLGM 222
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 49-221 4.92e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 40.30  E-value: 4.92e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  49 INALTHGNELCGAIAVDALlAAGVRPA--RGRLTLS-FANVEAYER--FDINDPDatrfiDEDLNRVWSADKlDGpgnSL 123
Cdd:cd18174    3 VTAGVHGYEYASIEALQRL-IKELDPAklSGTVIVVpIANIPAFEGrsIYVNPLD-----GKNLNRSFPGDP-DG---TP 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 124 ELRRAREL-RPVIDTVDYLLDIHS--MHEKAEP--LMLTGPHAKGVALGRLV----GAPsHLMIDAGHAAGRRLRDYGGF 194
Cdd:cd18174   73 TERLAHWLtTNVIARADYYIDLHGgdLNEDLRPfvYYYETGNAALDAASREMaeafGLD-HIVFYKARLKASRGSLYTQA 151

                        170       180
                 ....*....|....*....|....*..
gi 480892069 195 GDAASPKNALLIECGQHFERVSRDVAL 221
Cdd:cd18174  152 AALLRGIPAILVEAGGLGSRDEEDVAR 178
M14_ASTE_ASPA-like cd06252
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 43-185 8.05e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349470  Cd Length: 224  Bit Score: 40.25  E-value: 8.05e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069  43 PGPHVMINALTHGNELCGAIAVdALLAAGVRPA--RGRLT-LSFANVEAYErfdindpDATRF--IDE-DLNRVWSADKL 116
Cdd:cd06252   33 SGPTVLLTGGNHGDEYEGPIAL-RRLARDLDPEdvRGRLIiVPALNLPAVR-------AGTRTspLDGgNLNRAFPGDAD 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 480892069 117 DGPGNSLELRRARELRPVidtVDYLLDIHS------------MHEKAEPLMltgpHAKGVALGRLVGAPSHLMIDAGHAA 184
Cdd:cd06252  105 GTPTERIAHFLETVLLPR---ADAVIDLHSggssldfvpcaaVHLLPDPAQ----RARSLALAEAFGAPLSVVVDNVDAP 177


                 .
gi 480892069 185 G 185
Cdd:cd06252  178 G 178
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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