|
Name |
Accession |
Description |
Interval |
E-value |
| SpoVB |
COG5841 |
Stage V sporulation protein SpoVB/SpoIIIF, required for spore cortex synthesis [Cell cycle ... |
4-527 |
2.66e-132 |
|
Stage V sporulation protein SpoVB/SpoIIIF, required for spore cortex synthesis [Cell cycle control, cell division, chromosome partitioning];
Pssm-ID: 444543 [Multi-domain] Cd Length: 532 Bit Score: 394.91 E-value: 2.66e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 4 KEMRSMMQGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISG 80
Cdd:COG5841 1 MKKQSFLKGALILTAAGLISKILGAIYRIPLQNIIGDEGFGLYQMAYPIYGLFLTLATAGIPVAISKLVAEYNalgDYKG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 81 QKKILQQLFPFVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTE 159
Cdd:COG5841 81 ARRIFRVSLLLLLILGLVFFLLLFFGAPFIArLLLGDPRAYYSLRALAPALLIVPVMSVLRGYFQGLQNMTPTAVSQVIE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 160 QIVRVGVILIAAYSFSRFGwsVYQTGTIAMAGALFGGIVAAGILWYYDRKIR--VGSSAYLTQWKVEKGSRKLFGRLVVE 237
Cdd:COG5841 161 QIVRVIFILLLAYLLLPYG--LEYAAAGATFGAVIGALAGLLVLLYYYRKRKkkLKRLLEKSKTKSKESSKDILKELLRI 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 238 GGIVSLYSAYLILFQLIDSFLVKNSLVAAGISDAAAKVDKGVYD-RGQPLVQLGLVVALALSSSFLPALTKYFMSQENAR 316
Cdd:COG5841 239 AIPITLSSLVLPLFQLIDSFTVPRRLQAAGLSASEATSLYGILSgMAQPLVNLPTVLATALATSLVPAISEAFAKKDRKL 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 317 FLQAAKVFLRLTTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIG 396
Cdd:COG5841 319 VRRRINQALRLTLLIGLPAAVGLAVLAEPINTLLFGNPEGGGILAILAPSAIFLSLQQVTTGILQGLGKQKIPVINLLIG 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 397 LLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFLVVFSKKEINRFVVEHHFLKKLTISLGIMGFVLLCYQGVIAVLFgi 476
Cdd:COG5841 399 LLVKIILNYLLVPLFGIYGAALATVIGYLVAAILNLAALKKYTGFKLDLRRRLLKPLLASLIMGIVVLLLYRLLSLFL-- 476
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 486874377 477 VEHRGQALILTIVGVLMGGTTFIFTIVKIKLFTIREWLTLPF-GAKILRMKR 527
Cdd:COG5841 477 PESRLGALLATLIAVLVGAIVYLLLLLKLGLITKEELRRLPKgGKKLARLLR 528
|
|
| MATE_SpoVB_like |
cd13124 |
Stage V sporulation protein B, also known as Stage III sporulation protein F, and related ... |
14-440 |
2.82e-126 |
|
Stage V sporulation protein B, also known as Stage III sporulation protein F, and related proteins; The integral membrane protein SpoVB has been implicated in the biosynthesis of the peptidoglycan component of the spore cortex in Bacillus subtilis. This model represents a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).
Pssm-ID: 240529 [Multi-domain] Cd Length: 434 Bit Score: 376.04 E-value: 2.82e-126
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 14 AILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISGQKKILQQLFP 90
Cdd:cd13124 1 LILTIAGLISKILGAIYRIPLQNIIGDEGMGLYQMAYPIYGIFLTLATAGIPVAISKLVAEYNalgDYKGARRIFRVSLI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 91 FVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILI 169
Cdd:cd13124 81 LLLILGLVFFLLLFFGAPFLAqLLLGDPRLAPVIRALAPAILIVPLLSVLRGYFQGLQNMTPTAVSQVIEQIVRVIVILV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 170 AAYSFSRFGWSVYQTGTIAMAGALFGGIVAAGILWYYDRKIRVGSSAYLTQWKVEKGS-RKLFGRLVVEGGIVSLYSAYL 248
Cdd:cd13124 161 LAYLLLPVGGGLYYAAAGATFGAVIGALAGLLVLLYYYRKKRRELKRLLRKSPRSKIStKEILKELLSYAIPITLSSLIL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 249 ILFQLIDSFLVKNSLVAAGISDAAAKVDKGVY-DRGQPLVQLGLVVALALSSSFLPALTKYFMSQENARFLQAAKVFLRL 327
Cdd:cd13124 241 PLLQLIDSFTVPRRLQAAGLSESEATALYGILtGRAQPLVQLPTVIATALSTSLVPAISEAYAKGDKKELRRRINQALRL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 328 TTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPL 407
Cdd:cd13124 321 TLLIGLPAAVGLAVLAEPINTLLFGDSEGGLILQILAPSILFLSLQQVTAAILQGLGKVKIPVINLLIGLLVKIVLNYVL 400
|
410 420 430
....*....|....*....|....*....|...
gi 486874377 408 TYSFGTLGASISTITGLVVTLFFLVVFSKKEIN 440
Cdd:cd13124 401 IPIFGIYGAAIATVIGFLVAALLNLRALKKLLG 433
|
|
| spore_V_B |
TIGR02900 |
stage V sporulation protein B; SpoVB is the stage V sporulation protein B of the bacterial ... |
11-500 |
2.38e-28 |
|
stage V sporulation protein B; SpoVB is the stage V sporulation protein B of the bacterial endopore formation program in Bacillus subtilis and various other Firmcutes. It is nearly universal among endospore-formers. Paralogs with rather high sequence similarity to SpoVB exist, including YkvU in B. subtilis and a number of proteins in the genus Clostridium. [Cellular processes, Sporulation and germination]
Pssm-ID: 274343 [Multi-domain] Cd Length: 488 Bit Score: 118.16 E-value: 2.38e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 11 QGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISGQKKILQQ 87
Cdd:TIGR02900 3 KGTFILTIANLITRILGFIFRIVLSRILGAEGVGLYGMAMPIYFLFITLTTGGLPVAISKFVAEASaknDRKNIKKILKV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 88 LFPFVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGV 166
Cdd:TIGR02900 83 SLIFTLIWSLIVTAIVFLLSPFIAsTLLKDERSLYSLLVICPAMPFIALSSVLKGYFQGISNMKPPAYIQVIEQIVRISV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 167 ILIAAYSFSrfGWSVYQTGTIAMAGALFGGIVAAGILW-YYDRKIRVGSSAYLTQWKVEkgSRKLFGRLVVEGGIVSLYS 245
Cdd:TIGR02900 163 VALLISAFL--PYGLEYAVAGAYLSLVLGELVSLLYLYfFFKRKKSFSIRFPFFDYKSE--GKALLFDLFSVSLPLTLSR 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 246 AYLILFQLIDSFLVKNSLVAAGISDAAAKVDKGVYD-RGQPLVQLGLVVALALSSSFLPALTKYfMSQEN-----ARFLQ 319
Cdd:TIGR02900 239 FIGSLLYFLETLLVPQRLVIAGVTYREATSLYGKLSgMAMPLLTFPAVITSSLSTALVPDISEA-MAKKNyssieKRINQ 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 320 AAKVflrltTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVC-SIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLL 398
Cdd:TIGR02900 318 AIKI-----SLLLGLITTVILLVIPDELGALFYGRPDAGNFIRVLApSFPFLYFSAPLQSILQGLGKQKVALRNSLIGAI 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 399 VKLFLTSPLTY--SFGTLGASISTITGlVVTLFFLVVFSKKEINRFVVEHHFlKKLTISLGIMGFVLLCYQGVIAVLFGI 476
Cdd:TIGR02900 393 VKIILLFVLTSipSINIYGYAITFIIT-SVLVTILNLAEIKKNIRITIDLSD-FLIFALLGILVYLGLSLLKYIIFNPNI 470
|
490 500
....*....|....*....|....
gi 486874377 477 vehrgqaLILTIVGVLMGGTTFIF 500
Cdd:TIGR02900 471 -------LINLLLIIILGFSLYIL 487
|
|
| Polysacc_synt |
pfam01943 |
Polysaccharide biosynthesis protein; Members of this family are integral membrane proteins. ... |
11-309 |
2.12e-16 |
|
Polysaccharide biosynthesis protein; Members of this family are integral membrane proteins. Many members of the family are implicated in production of polysaccharide. The family includes RfbX part of the O antigen biosynthesis operon. The family includes SpoVB from Bacillus subtilis, which is involved in spore cortex biosynthesis.
Pssm-ID: 426523 [Multi-domain] Cd Length: 274 Bit Score: 79.27 E-value: 2.12e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 11 QGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQSDisgqKKILQQLFP 90
Cdd:pfam01943 3 KNVIVLLLSQLITYLLAFVTLPYLARILGPEGFGLYAFALAFVGLLTIFTDFGFNAALVREIAKYRE----KYELVSLYW 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 91 FVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQVVSFTFLLVP-ILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILI 169
Cdd:pfam01943 79 LNALLVKLVLVLLFLLLPLIAAFLGLPELAVLYRILALMFLLLPgVVQFRRGLFQGLERMKYALISQIVESLSRVILVFI 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 170 AAYSFSRFgwsvyqtgTIAMAGALFGGIVAAGILWYYDRKIRVGSSAYLtqwkvekgSRKLFGRLVVEGGIVSLYSAYLI 249
Cdd:pfam01943 159 AVFLPGDI--------VAALLYQVLAALVGGIIALAVLRKIFRPRFVFP--------SLKLVRKLLSFGAPLFISSLAIS 222
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 486874377 250 LFQLIDSFLvknslvaagISDAAAKVDKGVYDRGQPLVQLGL-VVALALSSSFLPALTKYF 309
Cdd:pfam01943 223 LYTYIDSIL---------LGLLLGAGAVGIYNAAYQLLVAIQgLLNPIVTAVLFPVFSKLQ 274
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| SpoVB |
COG5841 |
Stage V sporulation protein SpoVB/SpoIIIF, required for spore cortex synthesis [Cell cycle ... |
4-527 |
2.66e-132 |
|
Stage V sporulation protein SpoVB/SpoIIIF, required for spore cortex synthesis [Cell cycle control, cell division, chromosome partitioning];
Pssm-ID: 444543 [Multi-domain] Cd Length: 532 Bit Score: 394.91 E-value: 2.66e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 4 KEMRSMMQGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISG 80
Cdd:COG5841 1 MKKQSFLKGALILTAAGLISKILGAIYRIPLQNIIGDEGFGLYQMAYPIYGLFLTLATAGIPVAISKLVAEYNalgDYKG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 81 QKKILQQLFPFVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTE 159
Cdd:COG5841 81 ARRIFRVSLLLLLILGLVFFLLLFFGAPFIArLLLGDPRAYYSLRALAPALLIVPVMSVLRGYFQGLQNMTPTAVSQVIE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 160 QIVRVGVILIAAYSFSRFGwsVYQTGTIAMAGALFGGIVAAGILWYYDRKIR--VGSSAYLTQWKVEKGSRKLFGRLVVE 237
Cdd:COG5841 161 QIVRVIFILLLAYLLLPYG--LEYAAAGATFGAVIGALAGLLVLLYYYRKRKkkLKRLLEKSKTKSKESSKDILKELLRI 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 238 GGIVSLYSAYLILFQLIDSFLVKNSLVAAGISDAAAKVDKGVYD-RGQPLVQLGLVVALALSSSFLPALTKYFMSQENAR 316
Cdd:COG5841 239 AIPITLSSLVLPLFQLIDSFTVPRRLQAAGLSASEATSLYGILSgMAQPLVNLPTVLATALATSLVPAISEAFAKKDRKL 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 317 FLQAAKVFLRLTTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIG 396
Cdd:COG5841 319 VRRRINQALRLTLLIGLPAAVGLAVLAEPINTLLFGNPEGGGILAILAPSAIFLSLQQVTTGILQGLGKQKIPVINLLIG 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 397 LLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFLVVFSKKEINRFVVEHHFLKKLTISLGIMGFVLLCYQGVIAVLFgi 476
Cdd:COG5841 399 LLVKIILNYLLVPLFGIYGAALATVIGYLVAAILNLAALKKYTGFKLDLRRRLLKPLLASLIMGIVVLLLYRLLSLFL-- 476
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 486874377 477 VEHRGQALILTIVGVLMGGTTFIFTIVKIKLFTIREWLTLPF-GAKILRMKR 527
Cdd:COG5841 477 PESRLGALLATLIAVLVGAIVYLLLLLKLGLITKEELRRLPKgGKKLARLLR 528
|
|
| MATE_SpoVB_like |
cd13124 |
Stage V sporulation protein B, also known as Stage III sporulation protein F, and related ... |
14-440 |
2.82e-126 |
|
Stage V sporulation protein B, also known as Stage III sporulation protein F, and related proteins; The integral membrane protein SpoVB has been implicated in the biosynthesis of the peptidoglycan component of the spore cortex in Bacillus subtilis. This model represents a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).
Pssm-ID: 240529 [Multi-domain] Cd Length: 434 Bit Score: 376.04 E-value: 2.82e-126
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 14 AILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISGQKKILQQLFP 90
Cdd:cd13124 1 LILTIAGLISKILGAIYRIPLQNIIGDEGMGLYQMAYPIYGIFLTLATAGIPVAISKLVAEYNalgDYKGARRIFRVSLI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 91 FVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILI 169
Cdd:cd13124 81 LLLILGLVFFLLLFFGAPFLAqLLLGDPRLAPVIRALAPAILIVPLLSVLRGYFQGLQNMTPTAVSQVIEQIVRVIVILV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 170 AAYSFSRFGWSVYQTGTIAMAGALFGGIVAAGILWYYDRKIRVGSSAYLTQWKVEKGS-RKLFGRLVVEGGIVSLYSAYL 248
Cdd:cd13124 161 LAYLLLPVGGGLYYAAAGATFGAVIGALAGLLVLLYYYRKKRRELKRLLRKSPRSKIStKEILKELLSYAIPITLSSLIL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 249 ILFQLIDSFLVKNSLVAAGISDAAAKVDKGVY-DRGQPLVQLGLVVALALSSSFLPALTKYFMSQENARFLQAAKVFLRL 327
Cdd:cd13124 241 PLLQLIDSFTVPRRLQAAGLSESEATALYGILtGRAQPLVQLPTVIATALSTSLVPAISEAYAKGDKKELRRRINQALRL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 328 TTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPL 407
Cdd:cd13124 321 TLLIGLPAAVGLAVLAEPINTLLFGDSEGGLILQILAPSILFLSLQQVTAAILQGLGKVKIPVINLLIGLLVKIVLNYVL 400
|
410 420 430
....*....|....*....|....*....|...
gi 486874377 408 TYSFGTLGASISTITGLVVTLFFLVVFSKKEIN 440
Cdd:cd13124 401 IPIFGIYGAAIATVIGFLVAALLNLRALKKLLG 433
|
|
| spore_V_B |
TIGR02900 |
stage V sporulation protein B; SpoVB is the stage V sporulation protein B of the bacterial ... |
11-500 |
2.38e-28 |
|
stage V sporulation protein B; SpoVB is the stage V sporulation protein B of the bacterial endopore formation program in Bacillus subtilis and various other Firmcutes. It is nearly universal among endospore-formers. Paralogs with rather high sequence similarity to SpoVB exist, including YkvU in B. subtilis and a number of proteins in the genus Clostridium. [Cellular processes, Sporulation and germination]
Pssm-ID: 274343 [Multi-domain] Cd Length: 488 Bit Score: 118.16 E-value: 2.38e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 11 QGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQS---DISGQKKILQQ 87
Cdd:TIGR02900 3 KGTFILTIANLITRILGFIFRIVLSRILGAEGVGLYGMAMPIYFLFITLTTGGLPVAISKFVAEASaknDRKNIKKILKV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 88 LFPFVFVIAGLCWAFFFLGSHEIA-FMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGV 166
Cdd:TIGR02900 83 SLIFTLIWSLIVTAIVFLLSPFIAsTLLKDERSLYSLLVICPAMPFIALSSVLKGYFQGISNMKPPAYIQVIEQIVRISV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 167 ILIAAYSFSrfGWSVYQTGTIAMAGALFGGIVAAGILW-YYDRKIRVGSSAYLTQWKVEkgSRKLFGRLVVEGGIVSLYS 245
Cdd:TIGR02900 163 VALLISAFL--PYGLEYAVAGAYLSLVLGELVSLLYLYfFFKRKKSFSIRFPFFDYKSE--GKALLFDLFSVSLPLTLSR 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 246 AYLILFQLIDSFLVKNSLVAAGISDAAAKVDKGVYD-RGQPLVQLGLVVALALSSSFLPALTKYfMSQEN-----ARFLQ 319
Cdd:TIGR02900 239 FIGSLLYFLETLLVPQRLVIAGVTYREATSLYGKLSgMAMPLLTFPAVITSSLSTALVPDISEA-MAKKNyssieKRINQ 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 320 AAKVflrltTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVC-SIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLL 398
Cdd:TIGR02900 318 AIKI-----SLLLGLITTVILLVIPDELGALFYGRPDAGNFIRVLApSFPFLYFSAPLQSILQGLGKQKVALRNSLIGAI 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 399 VKLFLTSPLTY--SFGTLGASISTITGlVVTLFFLVVFSKKEINRFVVEHHFlKKLTISLGIMGFVLLCYQGVIAVLFGI 476
Cdd:TIGR02900 393 VKIILLFVLTSipSINIYGYAITFIIT-SVLVTILNLAEIKKNIRITIDLSD-FLIFALLGILVYLGLSLLKYIIFNPNI 470
|
490 500
....*....|....*....|....
gi 486874377 477 vehrgqaLILTIVGVLMGGTTFIF 500
Cdd:TIGR02900 471 -------LINLLLIIILGFSLYIL 487
|
|
| MATE_Wzx_like |
cd13128 |
Wzx, a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins; ... |
12-436 |
1.29e-20 |
|
Wzx, a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins; Escherichia coli Wzx and related proteins from other gram-negative bacteria are thought to act as flippases, assisting in the membrane translocation of lipopolysaccharides including those containing O-antigens. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).
Pssm-ID: 240533 [Multi-domain] Cd Length: 402 Bit Score: 93.76 E-value: 1.29e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 12 GAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEqSDISGQKKILQQLFPF 91
Cdd:cd13128 4 NSGWLFVGNIISKLLGFLVRVYLARYLGPEGFGILSLALAFVGLFSIFADLGLPTALVREIAR-YRKEKIREIISTSLVL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 92 VFVIAGLCWAFFFLGSHEIAFmmGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILIAA 171
Cdd:cd13128 83 KLILGILALLLLFLFAFLIAF--YDPELVLLLYILALSLPFSALNSLFRGIFQGFEKMKYIVIARIIESVLSLILALILV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 172 YsfsrFGWSVYQTGTIAMAGALFGGIVAagiLWYYDRKIRVGssayltqwKVEKGSRKLFGRLVVEGGIVSLYSAYLILF 251
Cdd:cd13128 161 L----LGGGLIGFALAYLIASVLSAILL---LYIYRKKILLP--------KFFSFDLKLAKELLRFSIPLALSGIFSLIY 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 252 QLIDSFLVKNSLvaagisdaaAKVDKGVYDRGQPLVQLGLVVALALSSSFLPALTKYFmSQENARFLQAAKVFLRLTTTI 331
Cdd:cd13128 226 TRIDTIMLGYFL---------GDAAVGIYNAAYRIAELLLFIPSAISTALFPRISELY-KNDKDELKKLLKKSFKYLFLI 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 332 AAAASIGLALLLPY-MNFALFKDY-KGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPLTY 409
Cdd:cd13128 296 SLPIAIGLFLFAEPiITLLFGEEYlPAALVLQILAWGFLFIFLNGIFGSILQAIGKQKLTLKILLIGALLNVILNLLLIP 375
|
410 420
....*....|....*....|....*..
gi 486874377 410 SFGTLGASISTITGLVVTLFFLVVFSK 436
Cdd:cd13128 376 KYGIIGAAIATLISEFIIFILLLYLLK 402
|
|
| Polysacc_synt |
pfam01943 |
Polysaccharide biosynthesis protein; Members of this family are integral membrane proteins. ... |
11-309 |
2.12e-16 |
|
Polysaccharide biosynthesis protein; Members of this family are integral membrane proteins. Many members of the family are implicated in production of polysaccharide. The family includes RfbX part of the O antigen biosynthesis operon. The family includes SpoVB from Bacillus subtilis, which is involved in spore cortex biosynthesis.
Pssm-ID: 426523 [Multi-domain] Cd Length: 274 Bit Score: 79.27 E-value: 2.12e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 11 QGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQSDisgqKKILQQLFP 90
Cdd:pfam01943 3 KNVIVLLLSQLITYLLAFVTLPYLARILGPEGFGLYAFALAFVGLLTIFTDFGFNAALVREIAKYRE----KYELVSLYW 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 91 FVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQVVSFTFLLVP-ILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILI 169
Cdd:pfam01943 79 LNALLVKLVLVLLFLLLPLIAAFLGLPELAVLYRILALMFLLLPgVVQFRRGLFQGLERMKYALISQIVESLSRVILVFI 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 170 AAYSFSRFgwsvyqtgTIAMAGALFGGIVAAGILWYYDRKIRVGSSAYLtqwkvekgSRKLFGRLVVEGGIVSLYSAYLI 249
Cdd:pfam01943 159 AVFLPGDI--------VAALLYQVLAALVGGIIALAVLRKIFRPRFVFP--------SLKLVRKLLSFGAPLFISSLAIS 222
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 486874377 250 LFQLIDSFLvknslvaagISDAAAKVDKGVYDRGQPLVQLGL-VVALALSSSFLPALTKYF 309
Cdd:pfam01943 223 LYTYIDSIL---------LGLLLGAGAVGIYNAAYQLLVAIQgLLNPIVTAVLFPVFSKLQ 274
|
|
| RfbX |
COG2244 |
Membrane protein involved in the export of O-antigen and teichoic acid [Cell wall/membrane ... |
6-432 |
3.13e-16 |
|
Membrane protein involved in the export of O-antigen and teichoic acid [Cell wall/membrane/envelope biogenesis];
Pssm-ID: 441845 [Multi-domain] Cd Length: 366 Bit Score: 80.38 E-value: 3.13e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 6 MRSMMQGAAILTIASFIAKVLSAVYRVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQSDiSGQKKIL 85
Cdd:COG2244 1 LKKLLKNTLWLLLGQLLGALLGFLLLPLLARLLGPEEYGLFALALSIVALLSVLADLGLSTALVRFIAEYRE-EELRRLL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 86 QQLFPFVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVG 165
Cdd:COG2244 80 STALLLRLLLSLLLALLLLLLAPFIAALLGEPELALLLLLLALALLLSALSAVLLALLRGLERFKLLALINILSSLLSLL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 166 VILIAAYSFSRFGWSVYQTGTIAMAGALFGgivaagilwyydrkirvgssayltqwkvekgsrklfgrlvveggivslys 245
Cdd:COG2244 160 LALLLALLGLGLWGLVLKYSLPLLLSGLLG-------------------------------------------------- 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 246 aylILFQLIDSFLVKNSLvaagiSDAAAkvdkGVYDRGQPLVQLGLVVALALSSSFLPALTKYFMSQENARFLQAAKVFL 325
Cdd:COG2244 190 ---LLLTNLDRLLLGALL-----GPAAV----GIYSAAYRLASLLLLLITALSQVLFPRLSRLLAEGDREELRRLYRKAL 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 326 RLTTTIAAAASIGLALLLPYMNFALFKD--YKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFL 403
Cdd:COG2244 258 RLLLLLGLPLALGLALLAPPLLSLLFGPeyAEAAPVLPILALGALFLALSGVLSNLLLALGRTRLLLIISLIGAVLNLVL 337
|
410 420
....*....|....*....|....*....
gi 486874377 404 TSPLTYSFGTLGASISTITGLVVTLFFLV 432
Cdd:COG2244 338 NLLLIPRYGLVGAAIATLLSYLVLLLLLL 366
|
|
| Polysacc_synt_C |
pfam14667 |
Polysaccharide biosynthesis C-terminal domain; This family represents the C-terminal integral ... |
359-507 |
6.68e-12 |
|
Polysaccharide biosynthesis C-terminal domain; This family represents the C-terminal integral membrane region of polysaccharide biosynthesis proteins.
Pssm-ID: 434111 [Multi-domain] Cd Length: 141 Bit Score: 62.98 E-value: 6.68e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 359 VLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFLVVFSKKE 438
Cdd:pfam14667 1 VLQILALSIIFLSLSTVLTSILQGLGRTKLALIALLIGALVKLILNLLLIPLFGIVGAAIATVISLLVVALLNLYYLRRY 80
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 486874377 439 INRFVVEHHFLKKLTISLGIMGFVLLCYQGVIAVLFGivehrgqaLILTIVGVLMGGTTFIFTIVKIKL 507
Cdd:pfam14667 81 LGLRLLLRRLLKPLLAALVMGIVVYLLLLLLGLLLGL--------ALALLLAVLVGALVYLLLLLLLKL 141
|
|
| MATE_tuaB_like |
cd13127 |
Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins; This ... |
49-440 |
7.58e-10 |
|
Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins; This family might function as a translocase for lipopolysaccharides and participate in the biosynthesis of cell wall components such as teichuronic acid. The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. A number of family members are involved in the synthesis of peptidoglycan components in bacteria.
Pssm-ID: 240532 [Multi-domain] Cd Length: 406 Bit Score: 60.96 E-value: 7.58e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 49 VYPIYGIAMTLALSGlpqFISKIVAEQSDisgQKKILQQLFPFVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQVVSF 128
Cdd:cd13127 41 ATVFIAFAQLFSDLG---LSSALIQRKDL---TEEDLSTVFWLNLLLGLLLYLLLFLLAPLIAAFYNEPELIPLLRVLSL 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 129 TFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILIAAYsfsrFGWSVYqtgtIAMAGALFGGIVAAGILWYYDR 208
Cdd:cd13127 115 SFLLSGLGSVPRALLQRELRFKKLAIIELISTLVSGVVAIVLAL----LGFGVW----ALVAQQLVGALVSTVLLWILSR 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 209 KIRVGSSAYltqwkveKGSRKLFGrlvveggivslYSAYLILFQLIDsFLVKNSLVAAgISDAAAKVDKGVYDRGQPLVQ 288
Cdd:cd13127 187 WRPRLSFSL-------ASLKELLS-----------FGSKLLGSNLLN-YFSRNLDNLI-IGKFLGAAALGYYSRAYRLAL 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 289 LGL-VVALALSSSFLPALTKyfMSQENARFLQAAKVFLRLTTTIAAAASIGLALLLPYMNFALFkdykGNG------VLG 361
Cdd:cd13127 247 LPVqNITGPITRVLFPALSR--LQDDPERLRRAYLKALRLLALITFPLMVGLALLAEPLVLLLL----GEKwlpavpILQ 320
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 486874377 362 VYVCSIAFMAVIQAYQSILQSRNRFFTsMLSAGIGLLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFLVVFSKKEIN 440
Cdd:cd13127 321 ILALAGLFQPLSSLNGSLLLALGRTDL-LLKLGLIKAVLTVLALLIGIPYGIIGVAIAYVIASLLSFPLNLYLAGRLIG 398
|
|
| MurJ |
COG0728 |
Lipid II flippase MurJ/MviN (peptidoglycan biosynthesis) [Cell wall/membrane/envelope ... |
177-507 |
2.73e-08 |
|
Lipid II flippase MurJ/MviN (peptidoglycan biosynthesis) [Cell wall/membrane/envelope biogenesis];
Pssm-ID: 440492 [Multi-domain] Cd Length: 503 Bit Score: 56.31 E-value: 2.73e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 177 FGWSVYQTGTIAMA-GALFGGIVAAGILWYYDRKIRVGssaYLTQWKVEKGSRKLFGRLvveGGIVSLYSAYLILFQLID 255
Cdd:COG0728 172 LLAPLFGPGIYALAwGVLLGGVLQLLIQLPALRRLGLR---LRPRFDLRDPGVRRVLKL---MLPALLGVSVSQINLLVD 245
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 256 SFLVkNSLVAAGISdaaakvdkgVYDRGQPLVQLGL-VVALALSSSFLPALTKYFMSQENARFLQAAKVFLRLTTTIAAA 334
Cdd:COG0728 246 TILA-SFLPEGSVS---------ALYYADRLYQLPLgLFGVAIGTALLPRLSRAAAAGDLEEFRRTLSRGLRLVLLLTLP 315
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 335 ASIGLALLLPYMNFALFKDYKGN--------GVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSP 406
Cdd:COG0728 316 AAVGLIVLAEPIVRLLFERGAFTaedtaltaLALAAYALGLPAFALVKVLARAFYARQDTRTPVRIAVIAVVVNIVLNLL 395
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 407 LTYSFGTLGASISTITGLVVTLFFLVVFSKKEINRFVVEH--HFLKKLTISLGIMGFVLLCYQGVIAVLFGIveHRGQAL 484
Cdd:COG0728 396 LVPPLGHAGLALATSLSALVNALLLLVLLRRRLGGLPLRGilRTLLKLLLASLVMGAVLWLLLRLLGDWLGG--GLLGRL 473
|
330 340
....*....|....*....|...
gi 486874377 485 ILTIVGVLMGGTTFIFTIVKIKL 507
Cdd:COG0728 474 LALALLILVGAAVYFALLLLLGV 496
|
|
| MATE_like_10 |
cd13125 |
Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins; This ... |
312-462 |
8.47e-07 |
|
Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins; This family might function as a translocase for lipopolysaccharides, such as O-antigen. The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. A number of family members are involved in the synthesis of peptidoglycan components in bacteria.
Pssm-ID: 240530 [Multi-domain] Cd Length: 409 Bit Score: 51.39 E-value: 8.47e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 312 QENARFLQAAkvfLRLTTTIAAAASIGLALLLPYMNFALFKDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSML 391
Cdd:cd13125 70 EKLARVWSTA---FRITLILSILGGLLLLLFSKPLSSLLFGDIDYSWVFILLGLALPLFALNNLLLAILNGLKEIKRYAL 146
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 486874377 392 SAGIGLLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFLVVFSKKE------INRFVVEHHFLKKLtISLGIMGFV 462
Cdd:cd13125 147 INIISSLIGLLVSVLLVYFYGLKGALLALVLNQSLIFLVTLIYSRKQpwfklkYLWGRLDKDIIKKL-LKFSLMALV 222
|
|
| MATE_like |
cd12082 |
Multidrug and toxic compound extrusion family and similar proteins; The integral membrane ... |
15-434 |
3.57e-06 |
|
Multidrug and toxic compound extrusion family and similar proteins; The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. MATE has also been identified as a large multigene family in plants, where the proteins are linked to disease resistance. A number of family members are involved in the synthesis of peptidoglycan components in bacteria.
Pssm-ID: 240527 [Multi-domain] Cd Length: 420 Bit Score: 49.24 E-value: 3.57e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 15 ILTIASFIAKVLSAVYrVPFQNLVGDEGFYVYQQVYPIYGIAMTLALSGLPQFISKIVAEQsDISGQKKILQQLFPFVFV 94
Cdd:cd12082 6 LSMLLQTLYNLVDTFF-LGRLLGDALAAVGLAFPLIALLIALGVGLSVGTSALISQAIGAG-DEEKARRVLVQSIVLAIL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 95 IAGLCWAFFFLGSHEIAFMMGDY-----RLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVILI 169
Cdd:cd12082 84 LGLLLAALLLFFSPLILSLLGAEeevieLAATYLTILILGLPITFLGAVLSGILQGEGDTRTAMIISVLSNLLNILLDPL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 170 AAYSFSRFGWSVyqtgTIAMAGALFGGIVAAGILWYYDRKIRVGSSAYLTQWKVekgSRKLFGRLVVEGGIVSLYSAYLI 249
Cdd:cd12082 164 LIFGLGPPELGI----AGAALATVISYVIGALLLLIYLRKGKKILKFKLSLLKP---DLELLRRLLRIGLPSAIQNSLLS 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 250 LFQLIDSFLVKNSLVAAGIsdaaakvdkGVYDRGQPLVQLGLVVALALSSSFLPALTKYFMSQENARFLQAAKVFLRLTT 329
Cdd:cd12082 237 LGLLIIVAIVAAFGGAAAL---------AAYTVAFRIASLAFMPALGLAQAALPVVGQNLGAGNFDRARRITWVALLIGL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 330 TIAAAASIgLALLLPYMNFALFKDYKGNGVLGVYVCSIA-----FMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLT 404
Cdd:cd12082 308 LIGAVLGL-LILLFPEPILSLFTNDPEFLELAVSYLRILaiyylFVGIGYVLQAGFQAAGRTLKSLIVSLLSYWVVRLPL 386
|
410 420 430
....*....|....*....|....*....|..
gi 486874377 405 SPL--TYSFGTLGASISTITGLVVTLFFLVVF 434
Cdd:cd12082 387 AYVlaYLGLGLYGIWLALVISLILAALVLLLF 418
|
|
| MATE_MurJ_like |
cd13123 |
MurJ/MviN, a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins; ... |
54-437 |
1.07e-05 |
|
MurJ/MviN, a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins; Escherichia coli MurJ (MviN) has been identified as essential for murein biosynthesis. It has been suggested that MurJ functions as the peptidoglycan lipid II flippase which is involved in translocation of lipid-anchored peptidoglycan precursors across the cytoplasmic membrane, though results obtained in Bacillus subtilis seem to indicate that its MurJ homologs are not essential for growth. Some MviN family members (e.g. in Mycobacterium tuberculosis) possess an extended C-terminal region that contains an intracellular pseudo-kinase domain and an extracellular domain resembling carbohydrate-binding proteins. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).
Pssm-ID: 240528 [Multi-domain] Cd Length: 420 Bit Score: 47.83 E-value: 1.07e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 54 GIAMTLALsgLPQFISKIvaEQSDISGQKKILQQLFPFVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQV-VSFTFLL 132
Cdd:cd13123 47 EGALSAAF--VPVFSEYL--EKEGEEEARRFASRVLTLLLLVLLLLTLLGILFAPLLVKLLAPGFSGDKFELaVALTRIM 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 133 VPILSFY------------RGNFqghllmVPSAVSQVteqIVRVGVILIAAYSFSRFGWSVYqtgtIAMAGALFGGIVAA 200
Cdd:cd13123 123 FPYLLFIslsallggilnaHGRF------FAPALAPV---LLNLVIIAGLLLLAPLFDLGIY----ALAWGVLLGGVLQL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 201 GILWYYDRKIRVGSSAYLtqWKVEKGSRKLFGRLvvegGIVSLYSAYLILFQLIDSFLVkNSLVAAGISdaaakvdkgVY 280
Cdd:cd13123 190 LVQLPALRRLGFRFRPRL--DFRDPGVRRVLKLM----LPALLGVSVSQINLLVDTILA-SFLPEGSIS---------AL 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 281 DRGQPLVQLGL-VVALALSSSFLPALTKYFMSQENARFLQAAKVFLRLTTTIAAAASIGLA--------LLLPYMNFALF 351
Cdd:cd13123 254 YYADRLYQLPLgIFGVAIATALLPRLSRLAAAGDLDEFRRTLSRGLRLVLFLLLPAAVGLIvlaepivrLLFERGAFTAE 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 352 KDYKGNGVLGVYVCSIAFMAVIQAYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFL 431
Cdd:cd13123 334 DTLMTASALAAYALGLPAFALVKLLSRAFYALKDTKTPVKIAVIAVAVNILLNLLLIKPLGHVGLALATSLSAWVNALLL 413
|
....*.
gi 486874377 432 VVFSKK 437
Cdd:cd13123 414 LVLLRR 419
|
|
| MurJ |
pfam03023 |
Lipid II flippase MurJ; Peptidoglycan synthesis (PG) biosynthesis involves the formation of ... |
73-431 |
2.43e-03 |
|
Lipid II flippase MurJ; Peptidoglycan synthesis (PG) biosynthesis involves the formation of peptidoglycan precursor lipid II (undecaprenyl-pyrophosphate-linked N-acetyl glucosamine-N-acetyl muramic acid-pentapeptide) on the cytosolic face of the cell membrane. Lipid II is then translocated across the membrane and its glycopeptide moiety becomes incorporated into the growing cell wall mesh. MviN, renamed as MurJ, is a lipid II flippase essential for cell wall peptidoglycan synthesis. MurJ belongs to the MVF (mouse virulence factor) family of MOP superfamily transporters, which also includes the MATE (multidrug and toxic compound extrusion) transporter and eukaryotic OLF (oligosaccharidyl-lipid flippase) families. In addition to the canonical MOP transporter core consisting of 12 transmembrane helices (TMs), MurJ has two additional C-terminal TMs (13 and 14) of unknown function. Structural analysis indicates that the N lobe (TMs 1-6) and C lobe (TMs 7-14) are arranged in an inward-facing N-shape conformation, rather than the outward-facing V-shape conformation observed in all existing MATE transporter structures. Furthermore, a hydrophobic groove is formed by two C-terminal transmembrane helices, which leads into a large central cavity that is mostly cationic. Mutagenesis studies, revealed a solvent-exposed cavity that is essential for function. Mutation of conserved residues (Ser17, Arg18, Arg24, Arg52, and Arg255) at the proximal site failed to complement MurJ function, consistent with the idea that these residues are important for recognizing the diphosphate and/or sugar moieties of lipid II. It has also been suggested that the chloride ion in the central cavity and a zinc ion at the beginning of TM 7 might be functionally important.
Pssm-ID: 397249 [Multi-domain] Cd Length: 451 Bit Score: 40.35 E-value: 2.43e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 73 AEQSDISGQKKILQQLFPFVFVIAGLCWAFFFLGSHEIAFMMGDYR----LAPLIQVVSFTFLLVPILSFYRGNFQGHLL 148
Cdd:pfam03023 44 LKAQDKEEAAEFVRKVSTLLILVLLLVTLIGILAAPWLIRLLAPGFdpetFSLAVSLLRITFPYLLLVSLSAVFGAVLNA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 149 MVPSAVSQVTEQIVRVGVILIAAYSFSRFGWSVYQTGtiamAGALFGGIVAAGILWYYDRKIrvgssayLTQWKVEKGSR 228
Cdd:pfam03023 124 RKKFFAPSFSPVLLNVGVILTLLLLANYLGNAIYALA----IGVLIGGVLQLLVQLPFLKKA-------GLLLKPRFGFR 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 229 -KLFGRLVVEGGIVSLYSAYLILFQLIDSFlvknslvaagISDAAAKVDKGVYDRGQPLVQLGL-VVALALSSSFLPALT 306
Cdd:pfam03023 193 dKGLKRFLKLMLPTLLGVSVSQLNLLIDTF----------LASFLAPGSVSYLYYADRIYQLPLgIFGVSISTVLLPRLS 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 307 KYFMSQENARFLQAAKVFLRLTTTIAAAASIGLALLLPYM--------NFALFKDYKGNGVLGVYVCSIAFMA----VIQ 374
Cdd:pfam03023 263 RHAADGDWDEFRDLLDQAIRLTLLLMIPVSFGLLVLSIPIvsllfergNFSPEDVTATAEVLAAYALGLIPYAlvklLSR 342
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 486874377 375 AYQSILQSRNRFFTSMLSAGIGLLVKLFLTSPLTYSFGTLGASISTITGLVVTLFFL 431
Cdd:pfam03023 343 VFYAREDTKTPFKISLISAVLNILLSLLLLPPLGVAGLALATSLSSLIGLVFLYYIL 399
|
|
| Polysacc_synt_3 |
pfam13440 |
Polysaccharide biosynthesis protein; |
88-346 |
9.44e-03 |
|
Polysaccharide biosynthesis protein;
Pssm-ID: 404342 [Multi-domain] Cd Length: 293 Bit Score: 38.34 E-value: 9.44e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 88 LFPFVFVIAGLCWAFFFLGSHEIAFMMGDYRLAPLIQVVSFTFLLVPILSFYRGNFQGHLLMVPSAVSQVTEQIVRVGVI 167
Cdd:pfam13440 50 VFTFNLIFGALIYLILFALAPLIASFYNQPELVPVLRVLALVLILNGLSSVQTALLRRDLRFKKLFKITLTSSVIGGIVG 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 168 LIAAYsfsrFGWSVYqtgtiAMA-GALFGGIVAAGILW-YYDRKIRVGSSAyltqwkveKGSRKLFGrlvveggivslYS 245
Cdd:pfam13440 130 IVLAY----LGFGVW-----ALViGTLAQQLLSLVLLWlASDWRPRLTFSL--------KVIKELFS-----------FG 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 486874377 246 AYLILFQLIDsFLVKNS---LVAAGISDAAAkvdkGVYDRGQPLVQLGL-VVALALSSSFLPALTKyfMSQENARFLQAA 321
Cdd:pfam13440 182 SWLLLSNLLN-YLARNLdnlIIGKVLGTAAL----GLYSVAYRLALLPTaNITSVITKVLFPALSR--IQDDKAKLRELY 254
|
250 260
....*....|....*....|....*
gi 486874377 322 KVFLRLTTTIAAAASIGLALLLPYM 346
Cdd:pfam13440 255 LRVLRLIAFIVFPIMAGLAVVAEPL 279
|
|
| |
