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Conserved domains on  [gi|508345021|gb|EOV16649|]
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hypothetical protein WGA_00003 [Escherichia coli KTE40]

Protein Classification

transposase( domain architecture ID 1000502)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DDE_3 super family cl46863
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 1-58 1.52e-31

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


The actual alignment was detected with superfamily member PHA02517:

Pssm-ID: 481203 [Multi-domain]  Cd Length: 277  Bit Score: 110.72  E-value: 1.52e-31
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTY 58
Cdd:PHA02517  62 VARCTVGRLMKELGLAGVLRGKKVRTTISRKAVAAPDRVNRQFVATRPNQLWVADFTY 119
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 1-58 1.52e-31

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 110.72  E-value: 1.52e-31
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTY 58
Cdd:PHA02517  62 VARCTVGRLMKELGLAGVLRGKKVRTTISRKAVAAPDRVNRQFVATRPNQLWVADFTY 119
transpos_IS3 NF033516
IS3 family transposase;
1-58 5.86e-19

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 78.38  E-value: 5.86e-19
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYKRG--GHEHVAIPNHLERQFAVTEPNQVWCGDVTY 58
Cdd:NF033516 166 VNHKRVYRLMRELGLLARRRRKRRPYTTdsGHVHPVAPNLLNRQFTATRPNQVWVTDITY 225
Tra5 COG2801
Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];
1-58 3.45e-13

Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442053 [Multi-domain]  Cd Length: 309  Bit Score: 62.09  E-value: 3.45e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYK-RGGHEHVAIPNHLerqFAVTEPNQVWCGDVTY 58
Cdd:COG2801  103 VNRKRVRRLMRELGLQARRRRKKKYTtYSGHGGPIAPNLL---FTATAPNQVWVTDITY 158
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 1-58 1.52e-31

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 110.72  E-value: 1.52e-31
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTY 58
Cdd:PHA02517  62 VARCTVGRLMKELGLAGVLRGKKVRTTISRKAVAAPDRVNRQFVATRPNQLWVADFTY 119
transpos_IS3 NF033516
IS3 family transposase;
1-58 5.86e-19

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 78.38  E-value: 5.86e-19
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYKRG--GHEHVAIPNHLERQFAVTEPNQVWCGDVTY 58
Cdd:NF033516 166 VNHKRVYRLMRELGLLARRRRKRRPYTTdsGHVHPVAPNLLNRQFTATRPNQVWVTDITY 225
Tra5 COG2801
Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];
1-58 3.45e-13

Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442053 [Multi-domain]  Cd Length: 309  Bit Score: 62.09  E-value: 3.45e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 508345021   1 MGRWLAGRLMKELGLVSCQQPTHRYK-RGGHEHVAIPNHLerqFAVTEPNQVWCGDVTY 58
Cdd:COG2801  103 VNRKRVRRLMRELGLQARRRRKKKYTtYSGHGGPIAPNLL---FTATAPNQVWVTDITY 158
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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