RecName: Full=Terpene synthase 1
Protein Classification
isoprenoid biosynthesis enzyme family protein( domain architecture ID 89)
isoprenoid biosynthesis enzyme family protein
List of domain hits
| Name | Accession | Description | Interval | E-value | |||||
| Isoprenoid_Biosyn_C1 super family | cl00210 | Isoprenoid Biosynthesis enzymes, Class 1; Superfamily of trans-isoprenyl diphosphate synthases ... |
6-313 | 2.04e-57 | |||||
Isoprenoid Biosynthesis enzymes, Class 1; Superfamily of trans-isoprenyl diphosphate synthases (IPPS) and class I terpene cyclases which either synthesis geranyl/farnesyl diphosphates (GPP/FPP) or longer chained products from isoprene precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), or use geranyl (C10)-, farnesyl (C15)-, or geranylgeranyl (C20)-diphosphate as substrate. These enzymes produce a myriad of precursors for such end products as steroids, cholesterol, sesquiterpenes, heme, carotenoids, retinoids, and diterpenes; and are widely distributed among archaea, bacteria, and eukaryota.The enzymes in this superfamily share the same 'isoprenoid synthase fold' and include several subgroups. The head-to-tail (HT) IPPS catalyze the successive 1'-4 condensation of the 5-carbon IPP to the growing isoprene chain to form linear, all-trans, C10-, C15-, C20- C25-, C30-, C35-, C40-, C45-, or C50-isoprenoid diphosphates. Cyclic monoterpenes, diterpenes, and sesquiterpenes, are formed from their respective linear isoprenoid diphosphates by class I terpene cyclases. The head-to-head (HH) IPPS catalyze the successive 1'-1 condensation of 2 farnesyl or 2 geranylgeranyl isoprenoid diphosphates. Cyclization of these 30- and 40-carbon linear forms are catalyzed by class II cyclases. Both the isoprenoid chain elongation reactions and the class I terpene cyclization reactions proceed via electrophilic alkylations in which a new carbon-carbon single bond is generated through interaction between a highly reactive electron-deficient allylic carbocation and an electron-rich carbon-carbon double bond. The catalytic site consists of a large central cavity formed by mostly antiparallel alpha helices with two aspartate-rich regions located on opposite walls. These residues mediate binding of prenyl phosphates via bridging Mg2+ ions, inducing proposed conformational changes that close the active site to solvent, stabilizing reactive carbocation intermediates. Generally, the enzymes in this family exhibit an all-trans reaction pathway, an exception, is the cis-trans terpene cyclase, trichodiene synthase. Mechanistically and structurally distinct, class II terpene cyclases and cis-IPPS are not included in this CD. The actual alignment was detected with superfamily member cd00687: Pssm-ID: 469660 [Multi-domain] Cd Length: 303 Bit Score: 189.11 E-value: 2.04e-57
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| Name | Accession | Description | Interval | E-value | |||||
| Terpene_cyclase_nonplant_C1 | cd00687 | Non-plant Terpene Cyclases, Class 1; This CD includes terpenoid cyclases such as pentalenene ... |
6-313 | 2.04e-57 | |||||
Non-plant Terpene Cyclases, Class 1; This CD includes terpenoid cyclases such as pentalenene synthase and aristolochene synthase which, using an all-trans pathway, catalyze the ionization of farnesyl diphosphate, followed by the formation of a macrocyclic intermediate by bond formation between C1 with either C10 (aristolochene synthase) or C11 (pentalenene synthase), resulting in production of tricyclic hydrocarbon pentalenene or bicyclic hydrocarbon aristolochene. As with other enzymes with the 'terpenoid synthase fold', they have two conserved metal binding motifs, proposed to coordinate Mg2+ ion-bridged binding of the diphosphate moiety of FPP to the enzymes. Metal-triggered substrate ionization initiates catalysis, and the alpha-barrel active site serves as a template to channel and stabilize the conformations of reactive carbocation intermediates through a complex cyclization cascade. These enzymes function in the monomeric form and are found in fungi, bacteria and Dictyostelium. Pssm-ID: 173835 [Multi-domain] Cd Length: 303 Bit Score: 189.11 E-value: 2.04e-57
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| Terpene_syn_C_2 | pfam19086 | Terpene synthase family 2, C-terminal metal binding; |
73-268 | 5.73e-23 | |||||
Terpene synthase family 2, C-terminal metal binding; Pssm-ID: 465972 [Multi-domain] Cd Length: 199 Bit Score: 94.98 E-value: 5.73e-23
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| Name | Accession | Description | Interval | E-value | |||||
| Terpene_cyclase_nonplant_C1 | cd00687 | Non-plant Terpene Cyclases, Class 1; This CD includes terpenoid cyclases such as pentalenene ... |
6-313 | 2.04e-57 | |||||
Non-plant Terpene Cyclases, Class 1; This CD includes terpenoid cyclases such as pentalenene synthase and aristolochene synthase which, using an all-trans pathway, catalyze the ionization of farnesyl diphosphate, followed by the formation of a macrocyclic intermediate by bond formation between C1 with either C10 (aristolochene synthase) or C11 (pentalenene synthase), resulting in production of tricyclic hydrocarbon pentalenene or bicyclic hydrocarbon aristolochene. As with other enzymes with the 'terpenoid synthase fold', they have two conserved metal binding motifs, proposed to coordinate Mg2+ ion-bridged binding of the diphosphate moiety of FPP to the enzymes. Metal-triggered substrate ionization initiates catalysis, and the alpha-barrel active site serves as a template to channel and stabilize the conformations of reactive carbocation intermediates through a complex cyclization cascade. These enzymes function in the monomeric form and are found in fungi, bacteria and Dictyostelium. Pssm-ID: 173835 [Multi-domain] Cd Length: 303 Bit Score: 189.11 E-value: 2.04e-57
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| Terpene_cyclase_C1 | cd00868 | Terpene cyclases, Class 1; Terpene cyclases, Class 1 (C1) of the class 1 family of isoprenoid ... |
63-308 | 5.36e-23 | |||||
Terpene cyclases, Class 1; Terpene cyclases, Class 1 (C1) of the class 1 family of isoprenoid biosynthesis enzymes, which share the 'isoprenoid synthase fold' and convert linear, all-trans, isoprenoids, geranyl (C10)-, farnesyl (C15)-, or geranylgeranyl (C20)-diphosphate into numerous cyclic forms of monoterpenes, diterpenes, and sesquiterpenes. Also included in this CD are the cis-trans terpene cyclases such as trichodiene synthase. The class I terpene cyclization reactions proceed via electrophilic alkylations in which a new carbon-carbon single bond is generated through interaction between a highly reactive electron-deficient allylic carbocation and an electron-rich carbon-carbon double bond. The catalytic site consists of a large central cavity formed by mostly antiparallel alpha helices with two aspartate-rich regions located on opposite walls. These residues mediate binding of prenyl phosphates via bridging Mg2+ ions, inducing proposed conformational changes that close the active site to solvent, stabilizing reactive carbocation intermediates. Mechanistically and structurally distinct, class II terpene cyclases and cis-IPPS are not included in this CD. Taxonomic distribution includes bacteria, fungi and plants. Pssm-ID: 173837 [Multi-domain] Cd Length: 284 Bit Score: 97.44 E-value: 5.36e-23
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| Terpene_syn_C_2 | pfam19086 | Terpene synthase family 2, C-terminal metal binding; |
73-268 | 5.73e-23 | |||||
Terpene synthase family 2, C-terminal metal binding; Pssm-ID: 465972 [Multi-domain] Cd Length: 199 Bit Score: 94.98 E-value: 5.73e-23
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