NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|374110734|sp|G2WJ80|]
View 

RecName: Full=Heme-responsive zinc finger transcription factor HAP1; AltName: Full=CYP1 activatory protein; AltName: Full=Heme activator protein 1

Protein Classification

GAL4 and fungal_TF_MHR domain-containing protein( domain architecture ID 11250913)

protein containing domains GAL4, ZIP_Hap1, and fungal_TF_MHR

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 59-99 3.76e-13

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


:

Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 65.07  E-value: 3.76e-13
                            10        20        30        40
                    ....*....|....*....|....*....|....*....|.
gi 374110734     59 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 99
Cdd:smart00066    2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
fungal_TF_MHR cd12148
fungal transcription factor regulatory middle homology region; This domain is present in the ...
 473-811 7.06e-08

fungal transcription factor regulatory middle homology region; This domain is present in the large family of fungal zinc cluster transcription factors that contain an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain. Examples of members of this large fungal group are the following Saccharomyces cerevisiae transcription factors, GAL4, STB5, DAL81, CAT8, RDR1, HAL9, PUT3, PPR1, ASG1, RSF2, PIP2, as well as the C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3. It has been suggested that this region plays a regulatory role.


:

Pssm-ID: 213391  Cd Length: 410  Bit Score: 56.68  E-value: 7.06e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  473 PPKRVIALFIEKFFKHLYPAIPILDEQNFKNHMNQMLSLSSmnptvnnfGMSMPSSSTLenqpitqinlpklsdscnlgi 552
Cdd:cd12148     1 PPREVADRLLDLYFENVHPLFPILHRPTFLRDLESLYSDPS--------SLSPASLALL--------------------- 51

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  553 liiilrltwlsipsnscevdlgeesgsFLVpnessnMSASALTSMAKEESLLLKHETPVEALELCQKYLIKFDELSnisn 632
Cdd:cd12148    52 ---------------------------LAV------LALAALSLPDSELRGESRRSLAERYYEAARQLLDLALFLP---- 94

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  633 nnVNLTTVQFAIFYNFYMKSASndlttltntnntgmanpghDSESHQILLSNITQMAFSCGLHRDPDNFPQLNatipats 712
Cdd:cd12148    95 --PSLETLQALLLLALYLLGTG-------------------DPSSAWLLLGLAIRLAQSLGLHRDPSSLPGLS------- 146

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  713 qdvsnngskkanpstnptlnnnmsaattnsssrsgsadsrsgsnpvnkkenqvsiERFKHTWRKIWYYIVSMDVNQSLSL 792
Cdd:cd12148   147 -------------------------------------------------------PFERELRRRLWWSLYILDRLLSLSL 171

                         330
                  ....*....|....*....
gi 374110734  793 GSPRLLRnlRDFSDTKLPS 811
Cdd:cd12148   172 GRPPSIS--DEDIDVPLPS 188
ZIP_Hap1 cd14655
Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; ...
 99-130 3.21e-05

Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; Hap1p mediates oxygen sensing and heme signaling in yeast. In response to heme, it promotes transcription of genes required for respiration and controlling oxidative damage. It is a member of the Gal4/Gal4p family of transcriptional activators which contain an N-terminal DNA binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Hap1p binds to DNA containing a direct repeat of two CGG triplets. It is a large protein that contains repression modules (RPMs) and heme-responsive motifs (HRMs) in addition to the DNA-binding and dimerization domains.


:

Pssm-ID: 271239  Cd Length: 32  Bit Score: 41.97  E-value: 3.21e-05
                          10        20        30
                  ....*....|....*....|....*....|..
gi 374110734   99 TWAEEAEKELLKDNELKKLRERVKSLEKTLSK 130
Cdd:cd14655     1 PWIEPVMKELAKGEEIKSLREEVKSLQKNRKK 32
 
Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 59-99 3.76e-13

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 65.07  E-value: 3.76e-13
                            10        20        30        40
                    ....*....|....*....|....*....|....*....|.
gi 374110734     59 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 99
Cdd:smart00066    2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
GAL4 cd00067
GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like ...
 59-95 8.70e-12

GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like GAL4. Domain consists of two helices organized around a Zn(2)Cys(6 )motif; Binds to sequences containing 2 DNA half sites comprised of 3-5 C/G combinations


Pssm-ID: 238023 [Multi-domain]  Cd Length: 36  Bit Score: 60.75  E-value: 8.70e-12
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 374110734   59 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGvaHLCHY 95
Cdd:cd00067     1 RVRRACDRCRRRKIKCDGEKPPCSNCIKLG--LECTY 35
Zn_clus pfam00172
Fungal Zn(2)-Cys(6) binuclear cluster domain;
63-99 4.68e-10

Fungal Zn(2)-Cys(6) binuclear cluster domain;


Pssm-ID: 395120 [Multi-domain]  Cd Length: 39  Bit Score: 56.15  E-value: 4.68e-10
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 374110734    63 SCTICRKRKVKCDKLRPHCQQCTKTGVAhlCHYMEQT 99
Cdd:pfam00172    2 ACDNCRKRKVKCDGKKPACSRCIKNGLE--CTYSRKS 36
fungal_TF_MHR cd12148
fungal transcription factor regulatory middle homology region; This domain is present in the ...
 473-811 7.06e-08

fungal transcription factor regulatory middle homology region; This domain is present in the large family of fungal zinc cluster transcription factors that contain an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain. Examples of members of this large fungal group are the following Saccharomyces cerevisiae transcription factors, GAL4, STB5, DAL81, CAT8, RDR1, HAL9, PUT3, PPR1, ASG1, RSF2, PIP2, as well as the C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3. It has been suggested that this region plays a regulatory role.


Pssm-ID: 213391  Cd Length: 410  Bit Score: 56.68  E-value: 7.06e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  473 PPKRVIALFIEKFFKHLYPAIPILDEQNFKNHMNQMLSLSSmnptvnnfGMSMPSSSTLenqpitqinlpklsdscnlgi 552
Cdd:cd12148     1 PPREVADRLLDLYFENVHPLFPILHRPTFLRDLESLYSDPS--------SLSPASLALL--------------------- 51

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  553 liiilrltwlsipsnscevdlgeesgsFLVpnessnMSASALTSMAKEESLLLKHETPVEALELCQKYLIKFDELSnisn 632
Cdd:cd12148    52 ---------------------------LAV------LALAALSLPDSELRGESRRSLAERYYEAARQLLDLALFLP---- 94

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  633 nnVNLTTVQFAIFYNFYMKSASndlttltntnntgmanpghDSESHQILLSNITQMAFSCGLHRDPDNFPQLNatipats 712
Cdd:cd12148    95 --PSLETLQALLLLALYLLGTG-------------------DPSSAWLLLGLAIRLAQSLGLHRDPSSLPGLS------- 146

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  713 qdvsnngskkanpstnptlnnnmsaattnsssrsgsadsrsgsnpvnkkenqvsiERFKHTWRKIWYYIVSMDVNQSLSL 792
Cdd:cd12148   147 -------------------------------------------------------PFERELRRRLWWSLYILDRLLSLSL 171

                         330
                  ....*....|....*....
gi 374110734  793 GSPRLLRnlRDFSDTKLPS 811
Cdd:cd12148   172 GRPPSIS--DEDIDVPLPS 188
ZIP_Hap1 cd14655
Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; ...
 99-130 3.21e-05

Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; Hap1p mediates oxygen sensing and heme signaling in yeast. In response to heme, it promotes transcription of genes required for respiration and controlling oxidative damage. It is a member of the Gal4/Gal4p family of transcriptional activators which contain an N-terminal DNA binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Hap1p binds to DNA containing a direct repeat of two CGG triplets. It is a large protein that contains repression modules (RPMs) and heme-responsive motifs (HRMs) in addition to the DNA-binding and dimerization domains.


Pssm-ID: 271239  Cd Length: 32  Bit Score: 41.97  E-value: 3.21e-05
                          10        20        30
                  ....*....|....*....|....*....|..
gi 374110734   99 TWAEEAEKELLKDNELKKLRERVKSLEKTLSK 130
Cdd:cd14655     1 PWIEPVMKELAKGEEIKSLREEVKSLQKNRKK 32
Fungal_trans smart00906
Fungal specific transcription factor domain; This domain is found in a number of fungal ...
 759-811 7.74e-03

Fungal specific transcription factor domain; This domain is found in a number of fungal transcription factors including transcriptional activator xlnR, yeast regulatory protein GAL4, and other transcription proteins regulating a variety of cellular and metabolic processes.


Pssm-ID: 214903 [Multi-domain]  Cd Length: 93  Bit Score: 37.43  E-value: 7.74e-03
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|...
gi 374110734    759 NKKENQVSIERFKHTWRKIWYyIVSMDVNQSLSLGSPRLLRNlrDFSDTKLPS 811
Cdd:smart00906   40 HRDPSNSDLEERERRRRRFWW-LYILDRLLSLSLGRPPLIDD--DDIDVPLPS 89
 
Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 59-99 3.76e-13

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 65.07  E-value: 3.76e-13
                            10        20        30        40
                    ....*....|....*....|....*....|....*....|.
gi 374110734     59 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 99
Cdd:smart00066    2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
GAL4 cd00067
GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like ...
 59-95 8.70e-12

GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like GAL4. Domain consists of two helices organized around a Zn(2)Cys(6 )motif; Binds to sequences containing 2 DNA half sites comprised of 3-5 C/G combinations


Pssm-ID: 238023 [Multi-domain]  Cd Length: 36  Bit Score: 60.75  E-value: 8.70e-12
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 374110734   59 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGvaHLCHY 95
Cdd:cd00067     1 RVRRACDRCRRRKIKCDGEKPPCSNCIKLG--LECTY 35
Zn_clus pfam00172
Fungal Zn(2)-Cys(6) binuclear cluster domain;
63-99 4.68e-10

Fungal Zn(2)-Cys(6) binuclear cluster domain;


Pssm-ID: 395120 [Multi-domain]  Cd Length: 39  Bit Score: 56.15  E-value: 4.68e-10
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 374110734    63 SCTICRKRKVKCDKLRPHCQQCTKTGVAhlCHYMEQT 99
Cdd:pfam00172    2 ACDNCRKRKVKCDGKKPACSRCIKNGLE--CTYSRKS 36
fungal_TF_MHR cd12148
fungal transcription factor regulatory middle homology region; This domain is present in the ...
 473-811 7.06e-08

fungal transcription factor regulatory middle homology region; This domain is present in the large family of fungal zinc cluster transcription factors that contain an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain. Examples of members of this large fungal group are the following Saccharomyces cerevisiae transcription factors, GAL4, STB5, DAL81, CAT8, RDR1, HAL9, PUT3, PPR1, ASG1, RSF2, PIP2, as well as the C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3. It has been suggested that this region plays a regulatory role.


Pssm-ID: 213391  Cd Length: 410  Bit Score: 56.68  E-value: 7.06e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  473 PPKRVIALFIEKFFKHLYPAIPILDEQNFKNHMNQMLSLSSmnptvnnfGMSMPSSSTLenqpitqinlpklsdscnlgi 552
Cdd:cd12148     1 PPREVADRLLDLYFENVHPLFPILHRPTFLRDLESLYSDPS--------SLSPASLALL--------------------- 51

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  553 liiilrltwlsipsnscevdlgeesgsFLVpnessnMSASALTSMAKEESLLLKHETPVEALELCQKYLIKFDELSnisn 632
Cdd:cd12148    52 ---------------------------LAV------LALAALSLPDSELRGESRRSLAERYYEAARQLLDLALFLP---- 94

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  633 nnVNLTTVQFAIFYNFYMKSASndlttltntnntgmanpghDSESHQILLSNITQMAFSCGLHRDPDNFPQLNatipats 712
Cdd:cd12148    95 --PSLETLQALLLLALYLLGTG-------------------DPSSAWLLLGLAIRLAQSLGLHRDPSSLPGLS------- 146

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 374110734  713 qdvsnngskkanpstnptlnnnmsaattnsssrsgsadsrsgsnpvnkkenqvsiERFKHTWRKIWYYIVSMDVNQSLSL 792
Cdd:cd12148   147 -------------------------------------------------------PFERELRRRLWWSLYILDRLLSLSL 171

                         330
                  ....*....|....*....
gi 374110734  793 GSPRLLRnlRDFSDTKLPS 811
Cdd:cd12148   172 GRPPSIS--DEDIDVPLPS 188
ZIP_Hap1 cd14655
Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; ...
 99-130 3.21e-05

Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; Hap1p mediates oxygen sensing and heme signaling in yeast. In response to heme, it promotes transcription of genes required for respiration and controlling oxidative damage. It is a member of the Gal4/Gal4p family of transcriptional activators which contain an N-terminal DNA binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Hap1p binds to DNA containing a direct repeat of two CGG triplets. It is a large protein that contains repression modules (RPMs) and heme-responsive motifs (HRMs) in addition to the DNA-binding and dimerization domains.


Pssm-ID: 271239  Cd Length: 32  Bit Score: 41.97  E-value: 3.21e-05
                          10        20        30
                  ....*....|....*....|....*....|..
gi 374110734   99 TWAEEAEKELLKDNELKKLRERVKSLEKTLSK 130
Cdd:cd14655     1 PWIEPVMKELAKGEEIKSLREEVKSLQKNRKK 32
Fungal_trans smart00906
Fungal specific transcription factor domain; This domain is found in a number of fungal ...
 759-811 7.74e-03

Fungal specific transcription factor domain; This domain is found in a number of fungal transcription factors including transcriptional activator xlnR, yeast regulatory protein GAL4, and other transcription proteins regulating a variety of cellular and metabolic processes.


Pssm-ID: 214903 [Multi-domain]  Cd Length: 93  Bit Score: 37.43  E-value: 7.74e-03
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|...
gi 374110734    759 NKKENQVSIERFKHTWRKIWYyIVSMDVNQSLSLGSPRLLRNlrDFSDTKLPS 811
Cdd:smart00906   40 HRDPSNSDLEERERRRRRFWW-LYILDRLLSLSLGRPPLIDD--DDIDVPLPS 89
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH