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Conserved domains on  [gi|1616990801|dbj|GDI61909|]
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enzyme IIB component of PTS [Escherichia coli]

Protein Classification

PTS sugar transporter subunit IIB; PTS fructose transporter subunit IIB( domain architecture ID 10793389)

PTS sugar transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming substrates (such as ascorbate, lactose, galactitol) concomitantly with their translocation across the cell membrane| PTS fructose transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of fructose concomitantly with its translocation across the cell membrane

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10427 PRK10427
PTS fructose-like transporter subunit IIB;
1-113 1.28e-71

PTS fructose-like transporter subunit IIB;


:

Pssm-ID: 182451  Cd Length: 114  Bit Score: 208.93  E-value: 1.28e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   1 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV 80
Cdd:PRK10427    2 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVKIETQGALGTENRLTDEDIRRADVVLLITDIELAGAERFEHCRYV 81

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1616990801  81 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 113
Cdd:PRK10427   82 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 114
 
Name Accession Description Interval E-value
PRK10427 PRK10427
PTS fructose-like transporter subunit IIB;
1-113 1.28e-71

PTS fructose-like transporter subunit IIB;


Pssm-ID: 182451  Cd Length: 114  Bit Score: 208.93  E-value: 1.28e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   1 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV 80
Cdd:PRK10427    2 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVKIETQGALGTENRLTDEDIRRADVVLLITDIELAGAERFEHCRYV 81

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1616990801  81 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 113
Cdd:PRK10427   82 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 114
FrwB COG1445
Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and ...
 3-100 4.32e-39

Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and metabolism];


Pssm-ID: 441054 [Multi-domain]  Cd Length: 99  Bit Score: 125.99  E-value: 4.32e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   3 YLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAgAERFEHCRYVQC 82
Cdd:COG1445     1 KIVAVTACPTGIAHTYMAAEALEKAAKELGHEIKVETQGSVGVENELTAEDIAAADAVILAADIEVD-MERFAGKPVIEV 79

                          90
                  ....*....|....*...
gi 1616990801  83 SIYAFLREPQRVMSAVRK 100
Cdd:COG1445    80 SVKDAIKDPEALIEKALA 97
PTS_IIB_fructose cd05569
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate: ...
 4-100 2.22e-35

PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99911 [Multi-domain]  Cd Length: 96  Bit Score: 116.43  E-value: 2.22e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   4 LVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGaERFEHCRYVQCS 83
Cdd:cd05569     1 IVAVTACPTGIAHTYMAAEALEKAAKKLGWEIKVETQGSLGIENELTAEDIAEADAVILAADVPVDD-ERFAGKRVYEVS 79

                          90
                  ....*....|....*..
gi 1616990801  84 IYAFLREPQRVMSAVRK 100
Cdd:cd05569    80 VAEAIKDAEAVIERALA 96
FRU TIGR00829
PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and ...
 5-84 3.29e-25

PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several PTS components of unknown specificities. The fructose components of this family phosphorylate fructose on the 1-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This family is specific for the IIB domain of the fructose PTS transporters. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 129909 [Multi-domain]  Cd Length: 85  Bit Score: 90.61  E-value: 3.29e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   5 VAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSI 84
Cdd:TIGR00829   1 VAVTACPTGIAHTFMAAEALEKAAKKRGWEVKVETQGSVGAQNALTAEDIAAADGVILAADREIDLSRRFAGKNVYETST 80
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 5-88 5.76e-16

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 66.97  E-value: 5.76e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   5 VAVTACVSGVAHTYMAAERLEKLCQLEKWgvsIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSI 84
Cdd:pfam02302   1 KILTACGAGMATSLMAAEALEKAAKELGI---VEAQGAAGVNELTAEDIADDADVVILAPDVAVEDLARFAGKPVYVIPV 77


                  ....
gi 1616990801  85 YAFL 88
Cdd:pfam02302  78 KDAL 81
 
Name Accession Description Interval E-value
PRK10427 PRK10427
PTS fructose-like transporter subunit IIB;
1-113 1.28e-71

PTS fructose-like transporter subunit IIB;


Pssm-ID: 182451  Cd Length: 114  Bit Score: 208.93  E-value: 1.28e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   1 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV 80
Cdd:PRK10427    2 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVKIETQGALGTENRLTDEDIRRADVVLLITDIELAGAERFEHCRYV 81

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1616990801  81 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 113
Cdd:PRK10427   82 QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE 114
FrwB COG1445
Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and ...
 3-100 4.32e-39

Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and metabolism];


Pssm-ID: 441054 [Multi-domain]  Cd Length: 99  Bit Score: 125.99  E-value: 4.32e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   3 YLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAgAERFEHCRYVQC 82
Cdd:COG1445     1 KIVAVTACPTGIAHTYMAAEALEKAAKELGHEIKVETQGSVGVENELTAEDIAAADAVILAADIEVD-MERFAGKPVIEV 79

                          90
                  ....*....|....*...
gi 1616990801  83 SIYAFLREPQRVMSAVRK 100
Cdd:COG1445    80 SVKDAIKDPEALIEKALA 97
PTS_IIB_fructose cd05569
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate: ...
 4-100 2.22e-35

PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99911 [Multi-domain]  Cd Length: 96  Bit Score: 116.43  E-value: 2.22e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   4 LVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGaERFEHCRYVQCS 83
Cdd:cd05569     1 IVAVTACPTGIAHTYMAAEALEKAAKKLGWEIKVETQGSLGIENELTAEDIAEADAVILAADVPVDD-ERFAGKRVYEVS 79

                          90
                  ....*....|....*..
gi 1616990801  84 IYAFLREPQRVMSAVRK 100
Cdd:cd05569    80 VAEAIKDAEAVIERALA 96
FRU TIGR00829
PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and ...
 5-84 3.29e-25

PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several PTS components of unknown specificities. The fructose components of this family phosphorylate fructose on the 1-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This family is specific for the IIB domain of the fructose PTS transporters. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 129909 [Multi-domain]  Cd Length: 85  Bit Score: 90.61  E-value: 3.29e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   5 VAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSI 84
Cdd:TIGR00829   1 VAVTACPTGIAHTFMAAEALEKAAKKRGWEVKVETQGSVGAQNALTAEDIAAADGVILAADREIDLSRRFAGKNVYETST 80
PRK09765 PRK09765
PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional
 4-95 7.69e-17

PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional


Pssm-ID: 182066 [Multi-domain]  Cd Length: 631  Bit Score: 74.39  E-value: 7.69e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   4 LVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCS 83
Cdd:PRK09765  166 IVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVP 245

                          90
                  ....*....|..
gi 1616990801  84 IYAFLREPQRVM 95
Cdd:PRK09765  246 VAEPIKHAEALI 257
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 5-88 5.76e-16

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 66.97  E-value: 5.76e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   5 VAVTACVSGVAHTYMAAERLEKLCQLEKWgvsIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSI 84
Cdd:pfam02302   1 KILTACGAGMATSLMAAEALEKAAKELGI---VEAQGAAGVNELTAEDIADDADVVILAPDVAVEDLARFAGKPVYVIPV 77


                  ....
gi 1616990801  85 YAFL 88
Cdd:pfam02302  78 KDAL 81
PRK11404 PRK11404
PTS fructose-like transporter subunit IIBC;
4-90 5.08e-15

PTS fructose-like transporter subunit IIBC;


Pssm-ID: 183120 [Multi-domain]  Cd Length: 482  Bit Score: 69.13  E-value: 5.08e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   4 LVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAE--RFEHCRYVQ 81
Cdd:PRK11404    6 IVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDraRFAGKKVYE 85


                  ....*....
gi 1616990801  82 CSIYAFLRE 90
Cdd:PRK11404   86 IAISQALKN 94
PRK10712 PRK10712
PTS system fructose-specific transporter subunits IIBC; Provisional
 4-68 6.16e-15

PTS system fructose-specific transporter subunits IIBC; Provisional


Pssm-ID: 236740 [Multi-domain]  Cd Length: 563  Bit Score: 69.00  E-value: 6.16e-15
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1616990801   4 LVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIEL 68
Cdd:PRK10712  106 VVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIEV 170
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 5-98 1.41e-12

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 58.42  E-value: 1.41e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801   5 VAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGalgtenrlADEDIRRADVALLITDIELagAERFEHCRYVQCSI 84
Cdd:cd00133     1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEAQG--------LSEVIDLADADLIISTVPL--AARFLGKPVIVVSP 70

                          90
                  ....*....|....
gi 1616990801  85 YAFLREPQRVMSAV 98
Cdd:cd00133    71 LLNEKDGEKILEKL 84
PRK10474 PRK10474
PTS fructose-like transporter subunit IIB;
19-103 1.78e-12

PTS fructose-like transporter subunit IIB;


Pssm-ID: 170468  Cd Length: 88  Bit Score: 58.35  E-value: 1.78e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1616990801  19 MAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAV 98
Cdd:PRK10474    1 MAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKI 80


                  ....*
gi 1616990801  99 RKVLS 103
Cdd:PRK10474   81 EAHLA 85
FrwC COG1299
Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and ...
 1-74 4.04e-05

Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and metabolism];


Pssm-ID: 440910 [Multi-domain]  Cd Length: 469  Bit Score: 41.03  E-value: 4.04e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1616990801   1 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERF 74
Cdd:COG1299     7 KVIVVATTTAGATAAAAAAAAAAAAAAAAGGVIVVVVGGGGGGGGNLLTTEEEAAAAAIIIAAADDVEDRFFGK 80
FrwC COG1299
Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and ...
 1-74 1.02e-03

Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and metabolism];


Pssm-ID: 440910 [Multi-domain]  Cd Length: 469  Bit Score: 36.79  E-value: 1.02e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1616990801   1 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERF 74
Cdd:COG1299     5 KKKVIVVATTTAGATAAAAAAAAAAAAAAAAGGVIVVVVGGGGGGGGNLLTTEEEAAAAAIIIAAADDVEDRFF 78
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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