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DNA ligase B [Enterobacter cloacae]
Protein Classification
NAD-dependent DNA ligase LigB ( domain architecture ID 11483082 )
NAD-dependent DNA ligase LigB catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction
List of domain hits
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
1-556
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 883.10
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 1 MVKWIS I LMIFL SS G A M A M CP V WSPA K A GQ EIAAL KA QL TR W NED YW K QG S SEV S D D VYD R L N ARL K QWQRCF H - D EP LH 79
Cdd:PRK08097 3 LWMALL I SLLLW SS S A W A V CP D WSPA R A QE EIAAL QQ QL AQ W DDA YW R QG K SEV D D E VYD Q L R ARL T QWQRCF G g P EP RD 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 80 DDR P AAS G T V K HP F AHTGV H K VES KQAL S RWMA TQQE LWVQPKVDGVAVTLVY KN GKL A QAISRG D GL Q GE E WTA Q AR I I 159
Cdd:PRK08097 83 VPL P PLN G K V L HP V AHTGV K K LAD KQAL A RWMA GRSD LWVQPKVDGVAVTLVY RD GKL V QAISRG N GL K GE D WTA K AR L I 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 160 PAIPQ T L A G P LAN S VLQGELFL L REGHIQQ R MGG M NARAKVAG A MMR ATDRAA L K Q T G I F I WAWP N GP KA M KA RL SA LA E 239
Cdd:PRK08097 163 PAIPQ Q L P G A LAN L VLQGELFL R REGHIQQ Q MGG I NARAKVAG L MMR KDPSPT L N Q I G V F V WAWP D GP AS M PE RL AQ LA T 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 240 AGF T LT A RYT L PVKN VAD V EAQ R TA W FK A S LPF A TDG I VVR ASA EPPG EQ W L PG E G S W V VAWKY L PV A QV T EV K A IH F T V 319
Cdd:PRK08097 243 AGF P LT Q RYT H PVKN AEE V ARW R ER W YR A P LPF V TDG V VVR QAK EPPG RY W Q PG Q G E W A VAWKY P PV Q QV A EV R A VQ F A V 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 320 GRTG R IT AIAQ LEP L MLDDKRV Q RV SL GSV N RWQ RL DIAPGDQVLVSLAGQGIPRLD N VVWR NVD R R KP Q PP - SS R YNG L 398
Cdd:PRK08097 323 GRTG K IT VVLE LEP V MLDDKRV S RV NI GSV R RWQ QW DIAPGDQVLVSLAGQGIPRLD K VVWR GAE R T KP T PP d AD R FHS L 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 399 T CF Y ASP E C M EQF F ARL T WLS SR Q A L DIE G V GE SG WR T L Y Q AHR FEHLFSWL Q LT QA QL TA TPGI SASHGAA LWHQFNLA 478
Cdd:PRK08097 403 S CF R ASP G C Q EQF L ARL V WLS GK Q G L GLD G I GE GT WR A L H Q TGL FEHLFSWL A LT PE QL AN TPGI GKARAEQ LWHQFNLA 482
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2131413628 479 R ER PF I RW IT A M GIPL ARST L R A AG DR T WQ A L SQ RSE AE W RM LPG V G QEK ARQ VVNW L HQ P QID ALA K WLAA EH I G GF 556
Cdd:PRK08097 483 R QQ PF S RW LK A L GIPL PQAA L N A LD DR S WQ Q L LS RSE QQ W QQ LPG I G EGR ARQ LIAF L QH P EVK ALA D WLAA QG I T GF 560
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
1-556
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 883.10
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 1 MVKWIS I LMIFL SS G A M A M CP V WSPA K A GQ EIAAL KA QL TR W NED YW K QG S SEV S D D VYD R L N ARL K QWQRCF H - D EP LH 79
Cdd:PRK08097 3 LWMALL I SLLLW SS S A W A V CP D WSPA R A QE EIAAL QQ QL AQ W DDA YW R QG K SEV D D E VYD Q L R ARL T QWQRCF G g P EP RD 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 80 DDR P AAS G T V K HP F AHTGV H K VES KQAL S RWMA TQQE LWVQPKVDGVAVTLVY KN GKL A QAISRG D GL Q GE E WTA Q AR I I 159
Cdd:PRK08097 83 VPL P PLN G K V L HP V AHTGV K K LAD KQAL A RWMA GRSD LWVQPKVDGVAVTLVY RD GKL V QAISRG N GL K GE D WTA K AR L I 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 160 PAIPQ T L A G P LAN S VLQGELFL L REGHIQQ R MGG M NARAKVAG A MMR ATDRAA L K Q T G I F I WAWP N GP KA M KA RL SA LA E 239
Cdd:PRK08097 163 PAIPQ Q L P G A LAN L VLQGELFL R REGHIQQ Q MGG I NARAKVAG L MMR KDPSPT L N Q I G V F V WAWP D GP AS M PE RL AQ LA T 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 240 AGF T LT A RYT L PVKN VAD V EAQ R TA W FK A S LPF A TDG I VVR ASA EPPG EQ W L PG E G S W V VAWKY L PV A QV T EV K A IH F T V 319
Cdd:PRK08097 243 AGF P LT Q RYT H PVKN AEE V ARW R ER W YR A P LPF V TDG V VVR QAK EPPG RY W Q PG Q G E W A VAWKY P PV Q QV A EV R A VQ F A V 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 320 GRTG R IT AIAQ LEP L MLDDKRV Q RV SL GSV N RWQ RL DIAPGDQVLVSLAGQGIPRLD N VVWR NVD R R KP Q PP - SS R YNG L 398
Cdd:PRK08097 323 GRTG K IT VVLE LEP V MLDDKRV S RV NI GSV R RWQ QW DIAPGDQVLVSLAGQGIPRLD K VVWR GAE R T KP T PP d AD R FHS L 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 399 T CF Y ASP E C M EQF F ARL T WLS SR Q A L DIE G V GE SG WR T L Y Q AHR FEHLFSWL Q LT QA QL TA TPGI SASHGAA LWHQFNLA 478
Cdd:PRK08097 403 S CF R ASP G C Q EQF L ARL V WLS GK Q G L GLD G I GE GT WR A L H Q TGL FEHLFSWL A LT PE QL AN TPGI GKARAEQ LWHQFNLA 482
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2131413628 479 R ER PF I RW IT A M GIPL ARST L R A AG DR T WQ A L SQ RSE AE W RM LPG V G QEK ARQ VVNW L HQ P QID ALA K WLAA EH I G GF 556
Cdd:PRK08097 483 R QQ PF S RW LK A L GIPL PQAA L N A LD DR S WQ Q L LS RSE QQ W QQ LPG I G EGR ARQ LIAF L QH P EVK ALA D WLAA QG I T GF 560
LIGANc
smart00532 Ligase N family;
30-423
4.16e-109
Ligase N family;
Pssm-ID: 214709 [Multi-domain]
Cd Length: 441
Bit Score: 333.05
E-value: 4.16e-109
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 30 Q EI AA L KAQ L TRWNED Y WKQGSSEV SD DV YDRL NAR LK QWQRCF ----- H D E P LHDDRPAASG --- T V K HP FAHTGVHKV 101
Cdd:smart00532 3 K EI SE L RKL L NQHDYR Y YVLDAPII SD AE YDRL MRE LK ELEEKH pelkt P D S P TQRVGGKPLE gfn K V R HP VPMLSLDNA 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 102 ESKQA L SRWM ------- ATQQELW V Q PK V DG VA V T L V Y K NGKL A QA IS RGDG LQ GE EW T AQARI I PA IP QT L A G PLA - NS 173
Cdd:smart00532 83 FDEDE L RAFD eriekal GSPFAYV V E PK I DG LS V S L L Y E NGKL V QA AT RGDG TV GE DV T QNVKT I RS IP LR L S G DVP e RL 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 174 VLQ GE L F LLR E G ---- HIQQRMG G M ---- N A R AKV AG AMMRATD R - A A LKQTGI F IWAWPN G ----- PK AMKAR L SA L A E 239
Cdd:smart00532 163 EVR GE V F MPK E D flal NEELEEE G E kpfa N P R NAA AG SLRQLDP R i T A KRKLRA F FYGLGT G eelfl PK TQSEA L KW L K E 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 240 A GF TLTARYT L p V KN VAD V EAQRTA W FK -- A S LP FAT DG I VV RASAEPPGEQ -- WLPGEGS W VV A W K YLPVAQV T EVKA I 315
Cdd:smart00532 243 L GF PVSPHTR L - C KN ADE V IEYYEE W EE kr A E LP YEI DG V VV KVDDLALQRE lg FTSKAPR W AI A Y K FPAEEAE T KLLD I 321
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 316 HFT VGRTG R IT AI A Q LEP LM L DDKR V Q R VS L GSVNRWQRL DI AP GD Q V L V SL AG QG IP RLDN VV W -- R NV D R R KPQP P SS 393
Cdd:smart00532 322 IVQ VGRTG K IT PV A E LEP VF L AGST V S R AT L HNEDEIEEK DI RI GD T V V V RK AG DV IP KVVG VV K ek R PG D E R EIEM P TH 401
410 420 430 440
....*....|....*....|....*....|....*....|
gi 2131413628 394 --------- R YN G LTCFYAS - P E C ME Q FFA R LTWLS SR Q A 423
Cdd:smart00532 402 cpscgselv R EE G EVDIRCP n P L C PA Q LIE R IIHFA SR K A 441
LIGANc
cd00114 NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining ...
30-303
6.26e-78
NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor, but using the same basic reaction mechanism. The enzyme reacts with the cofactor to form a phosphoamide-linked AMP with the amino group of a conserved Lysine in the KXDG motif, and subsequently transfers it to the DNA substrate to yield adenylated DNA. This alignment contains members of the NAD+ dependent subfamily only.
Pssm-ID: 238062 [Multi-domain]
Cd Length: 307
Bit Score: 247.89
E-value: 6.26e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 30 QE IA A L KAQ L TRWNED Y WKQGSSE VSD DV YDRL NAR L KQWQRCFHDEPLH D ------- DR P AAS - GT V K HP FAHTGVHKV 101
Cdd:cd00114 1 ER IA E L REL L NKHDYR Y YVLDEPS VSD AE YDRL YRE L RALEEEHPELKTP D sptqrvg GT P LSG f KK V R HP VPMLSLDNA 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 102 ESKQA L SRWMA ------- TQQELW V Q PK V DG VAVT L V Y K NG K L A QA IS RGDG LQ GE EW T AQA R I I PA IP Q TLAG PLANSV 174
Cdd:cd00114 81 FDEEE L RAFDE rikrflg EEPAYV V E PK I DG LSIS L R Y E NG V L V QA AT RGDG TT GE DV T ENV R T I RS IP L TLAG APETLE 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 175 LQ GE L F LLREGHIQQRM -------- GGM N A R AKV AG AMMRATDR - A A LKQTGI FI WAWPN ---- GPK AMKAR L SA L A E A G 241
Cdd:cd00114 161 VR GE V F MPKADFEALNK ereergek PFA N P R NAA AG SLRQLDPK i T A KRPLRF FI YGLGE aegl GPK TQSEA L AF L K E W G 240
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2131413628 242 F TLTARYT L p V KN VAD V E A -- QRTAWFKA SLP FAT DG I VV RASAEPPGEQWLPGEGS -- W VV A W K Y 303
Cdd:cd00114 241 F PVSPETR L - C KN IEE V L A fy DEIEAKRD SLP YEI DG V VV KVDDLALQRELGFTSKA pr W AI A Y K F 305
dnlj
TIGR00575 DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent ...
35-540
3.16e-66
DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent DNA ligases. Functions of these proteins include DNA repair, DNA replication, and DNA recombination. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). The member of this family from Treponema pallidum differs in having three rather than just one copy of the BRCT (BRCA1 C Terminus) domain (pfam00533) at the C-terminus. It is included in the seed. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273148 [Multi-domain]
Cd Length: 652
Bit Score: 227.17
E-value: 3.16e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 35 L KAQLTRWNED Y WKQGSSEV SD DV YDRL NAR L KQWQR c F H D E PLHD D R P --------- AASGT V K H PFAHTGVHKVESKQ 105
Cdd:TIGR00575 1 L RKLIRHHDYR Y YVLDEPSI SD AE YDRL YRE L QELEE - K H P E LITP D S P tqrvgaapl SRFPK V R H STPMLSLDNAFDED 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 106 A L SRW ------- MATQQ E LW V Q PK V DG VA V T L V Y K NG K L AQ A IS RGDG LQ GE EW TA QA R I I PA IP QT LAG PLANSV L Q -- 176
Cdd:TIGR00575 80 E L AAF ikrirrq LGLKV E YV V E PK I DG LS V S L T Y E NG V L VR A LT RGDG TV GE DV TA NV R T I RS IP LR LAG DNPPER L E vr 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 177 GE L F LLR E GH ----- IQQRM G GM --- N A R AKV AG AMMRATD R - A A LKQTGI F IWAWPN G P ---- KAMKAR L SA L AEA GF T 243
Cdd:TIGR00575 160 GE V F MPK E DF ealne ERREQ G EK pfa N P R NAA AG SLRQLDP R i T A KRKLRF F AYGLGE G L elpd ATQYEA L AW L KKW GF P 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 244 LTARYT L p VKNVAD V EAQRTAWFK -- A SLP FAT DG I VV R ----------- ASAEP P geqwlpgeg S W VV A W K YLPVAQV T 310
Cdd:TIGR00575 240 VSPHIR L - CDSIEE V LEYYREIEE kr D SLP YEI DG V VV K vddlalqdelg FTSKA P --------- R W AI A Y K FPAEEAQ T 309
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 311 EVKAIHFT VGRTG R IT AI A Q LEP LMLDDKR V Q R VS L GSVNRWQR LDI AP GD Q V L V SL AG QG IP RLDN V V -- W R NVDR R KP 388
Cdd:TIGR00575 310 KLLDVVVQ VGRTG A IT PV A K LEP VFVAGTT V S R AT L HNEDEIEE LDI RI GD T V V V RK AG DV IP KVVR V L le K R TGSE R PI 389
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 389 QP P SS --------- RYNGLTCFYA - SPE C ME Q FFA R LTWLS SR Q A L DIEG V G ESGWRT L YQA --- HRFEH L FS wlq L TQA 455
Cdd:TIGR00575 390 RF P TH cpscgsplv KIEEEAVIRC p NLN C PA Q RVE R IKHFA SR N A M DIEG L G DKVIEQ L FEK klv RSVAD L YA --- L KKE 466
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 456 Q L TATP G ISASHGAA L WHQFNLAR E R P FI R WIT A M GI P ----- L A RSTLRAA G dr T WQA L SQR S EA E WRMLP GVG QEK A R 530
Cdd:TIGR00575 467 D L LELE G FGEKSAQN L LNAIEKSK E K P LA R LLF A L GI R hvgev T A KNLAKHF G -- T LDK L KAA S LE E LLSVE GVG PKV A E 544
570
....*....|
gi 2131413628 531 QV VN WL H Q P Q 540
Cdd:TIGR00575 545 SI VN FF H D P N 554
Lig
COG0272 NAD-dependent DNA ligase [Replication, recombination and repair];
24-550
4.70e-64
NAD-dependent DNA ligase [Replication, recombination and repair];
Pssm-ID: 440042 [Multi-domain]
Cd Length: 668
Bit Score: 221.44
E-value: 4.70e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 24 S P AK A GQE I AA L KAQ L T R W N ED Y WKQGSS E V SD DV YD R L NAR L KQWQR c F H D E PLHD D R P ------- AAS G -- T V K H P -- 92
Cdd:COG0272 2 T P EE A KER I EE L REE L R R H N YR Y YVLDAP E I SD AE YD A L LRE L QALEA - E H P E LITP D S P tqrvgga PLE G fa K V R H A vp 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 93 ------- F ahtgvhkve S KQA L SRWMA -------- TQQ E LWVQP K V DG V A VT L V Y K NG K L AQ A IS RGDG LQ GE EW TA QA R 157
Cdd:COG0272 81 mlsldna F --------- S EEE L RDFDR rvrkflgd EPV E YVCEL K I DG L A IS L R Y E NG R L VR A AT RGDG TV GE DV TA NV R 151
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 158 I I PA IP QT L A G PLANS VL Q -- GE LFLLREGHIQ qrmgg M N ARAKV AG AMMR A TD R -- AA -- L K Q ----- T ----- GI F IW 221
Cdd:COG0272 152 T I RS IP LR L K G DDVPE VL E vr GE VYMPKADFEA ----- L N EEREE AG EKPF A NP R na AA gs L R Q ldpki T akrpl DF F AY 226
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 222 AW ---- PNG P KAMKAR L SA L A E A GF TLTARYTL p V K NVAD V E A QRTA W FK -- A SLP FAT DG I V V --------------- R 280
Cdd:COG0272 227 GL geve GLL P DTQSEA L EL L K E W GF PVNPERRV - C K SIEE V L A YIEE W EE kr H SLP YEI DG V V I kvndlalqerlgfts R 305
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 281 A sae P pgeqwlpgeg S W VV A W K YLPVAQV T EVKA I HFT VGRTG RI T AI A Q LEP LMLDDKR V Q R VS L GSVNRWQ R L D IAP G 360
Cdd:COG0272 306 A --- P ---------- R W AI A Y K FPAEEAT T KLLD I EVQ VGRTG AL T PV A R LEP VFVAGVT V S R AT L HNEDEIE R K D VRI G 372
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 361 D Q V L V SL AG QG IP RLDN VV wrn VDR R kpq P PSSRY -------------------------- NGL T C F yaspecm E Q FFA R 414
Cdd:COG0272 373 D T V V V RK AG DV IP EVVG VV --- LEK R --- P GDEKP fvmpthcpvcgsplvreegeaalrct NGL S C P ------- A Q LKE R 439
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 415 L TWLS SR Q A L DIEG V GE SGWRT L YQ A --- HRFEH L FS wlq LT QAQ L TATPGI --- SA S hgaalwhqf NL ------ ARER P 482
Cdd:COG0272 440 L KHFA SR K A M DIEG L GE KLIEQ L VD A glv KDPAD L YR --- LT KED L LGLERM gek SA Q --------- NL laaiek SKKT P 507
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2131413628 483 FI R WIT A M GI PL ----- A RSTL R AA G dr TWQ AL SQR SE A E WRMLP G V G QEK A RQV V NWLHQ P Q - IDALAKWL AA 550
Cdd:COG0272 508 LA R FLF A L GI RH vgett A KLLA R HF G -- SLD AL MAA SE E E LAAVD G I G PVV A ESI V EFFAE P H n RELIERLR AA 579
DNA_ligase_OB
pfam03120 NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the ...
309-381
7.25e-29
NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This family is a small domain found after the adenylation domain pfam01653 in NAD dependent ligases. OB-fold domains generally are involved in nucleic acid binding.
Pssm-ID: 460813 [Multi-domain]
Cd Length: 79
Bit Score: 109.38
E-value: 7.25e-29
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2131413628 309 V T EVKA I H F T VGRTG R IT AI A Q LEP LM L DDKR V Q R VS L GSVNRWQ R L DI AP GD Q V L V SL AG QG IP RLDN VV WR 381
Cdd:pfam03120 1 E T KLLD I E F Q VGRTG A IT PV A V LEP VE L AGTT V S R AT L HNEDEIK R K DI RI GD T V I V RK AG DV IP EVVG VV LE 73
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
1-556
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 883.10
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 1 MVKWIS I LMIFL SS G A M A M CP V WSPA K A GQ EIAAL KA QL TR W NED YW K QG S SEV S D D VYD R L N ARL K QWQRCF H - D EP LH 79
Cdd:PRK08097 3 LWMALL I SLLLW SS S A W A V CP D WSPA R A QE EIAAL QQ QL AQ W DDA YW R QG K SEV D D E VYD Q L R ARL T QWQRCF G g P EP RD 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 80 DDR P AAS G T V K HP F AHTGV H K VES KQAL S RWMA TQQE LWVQPKVDGVAVTLVY KN GKL A QAISRG D GL Q GE E WTA Q AR I I 159
Cdd:PRK08097 83 VPL P PLN G K V L HP V AHTGV K K LAD KQAL A RWMA GRSD LWVQPKVDGVAVTLVY RD GKL V QAISRG N GL K GE D WTA K AR L I 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 160 PAIPQ T L A G P LAN S VLQGELFL L REGHIQQ R MGG M NARAKVAG A MMR ATDRAA L K Q T G I F I WAWP N GP KA M KA RL SA LA E 239
Cdd:PRK08097 163 PAIPQ Q L P G A LAN L VLQGELFL R REGHIQQ Q MGG I NARAKVAG L MMR KDPSPT L N Q I G V F V WAWP D GP AS M PE RL AQ LA T 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 240 AGF T LT A RYT L PVKN VAD V EAQ R TA W FK A S LPF A TDG I VVR ASA EPPG EQ W L PG E G S W V VAWKY L PV A QV T EV K A IH F T V 319
Cdd:PRK08097 243 AGF P LT Q RYT H PVKN AEE V ARW R ER W YR A P LPF V TDG V VVR QAK EPPG RY W Q PG Q G E W A VAWKY P PV Q QV A EV R A VQ F A V 322
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 320 GRTG R IT AIAQ LEP L MLDDKRV Q RV SL GSV N RWQ RL DIAPGDQVLVSLAGQGIPRLD N VVWR NVD R R KP Q PP - SS R YNG L 398
Cdd:PRK08097 323 GRTG K IT VVLE LEP V MLDDKRV S RV NI GSV R RWQ QW DIAPGDQVLVSLAGQGIPRLD K VVWR GAE R T KP T PP d AD R FHS L 402
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 399 T CF Y ASP E C M EQF F ARL T WLS SR Q A L DIE G V GE SG WR T L Y Q AHR FEHLFSWL Q LT QA QL TA TPGI SASHGAA LWHQFNLA 478
Cdd:PRK08097 403 S CF R ASP G C Q EQF L ARL V WLS GK Q G L GLD G I GE GT WR A L H Q TGL FEHLFSWL A LT PE QL AN TPGI GKARAEQ LWHQFNLA 482
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2131413628 479 R ER PF I RW IT A M GIPL ARST L R A AG DR T WQ A L SQ RSE AE W RM LPG V G QEK ARQ VVNW L HQ P QID ALA K WLAA EH I G GF 556
Cdd:PRK08097 483 R QQ PF S RW LK A L GIPL PQAA L N A LD DR S WQ Q L LS RSE QQ W QQ LPG I G EGR ARQ LIAF L QH P EVK ALA D WLAA QG I T GF 560
LIGANc
smart00532 Ligase N family;
30-423
4.16e-109
Ligase N family;
Pssm-ID: 214709 [Multi-domain]
Cd Length: 441
Bit Score: 333.05
E-value: 4.16e-109
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 30 Q EI AA L KAQ L TRWNED Y WKQGSSEV SD DV YDRL NAR LK QWQRCF ----- H D E P LHDDRPAASG --- T V K HP FAHTGVHKV 101
Cdd:smart00532 3 K EI SE L RKL L NQHDYR Y YVLDAPII SD AE YDRL MRE LK ELEEKH pelkt P D S P TQRVGGKPLE gfn K V R HP VPMLSLDNA 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 102 ESKQA L SRWM ------- ATQQELW V Q PK V DG VA V T L V Y K NGKL A QA IS RGDG LQ GE EW T AQARI I PA IP QT L A G PLA - NS 173
Cdd:smart00532 83 FDEDE L RAFD eriekal GSPFAYV V E PK I DG LS V S L L Y E NGKL V QA AT RGDG TV GE DV T QNVKT I RS IP LR L S G DVP e RL 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 174 VLQ GE L F LLR E G ---- HIQQRMG G M ---- N A R AKV AG AMMRATD R - A A LKQTGI F IWAWPN G ----- PK AMKAR L SA L A E 239
Cdd:smart00532 163 EVR GE V F MPK E D flal NEELEEE G E kpfa N P R NAA AG SLRQLDP R i T A KRKLRA F FYGLGT G eelfl PK TQSEA L KW L K E 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 240 A GF TLTARYT L p V KN VAD V EAQRTA W FK -- A S LP FAT DG I VV RASAEPPGEQ -- WLPGEGS W VV A W K YLPVAQV T EVKA I 315
Cdd:smart00532 243 L GF PVSPHTR L - C KN ADE V IEYYEE W EE kr A E LP YEI DG V VV KVDDLALQRE lg FTSKAPR W AI A Y K FPAEEAE T KLLD I 321
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 316 HFT VGRTG R IT AI A Q LEP LM L DDKR V Q R VS L GSVNRWQRL DI AP GD Q V L V SL AG QG IP RLDN VV W -- R NV D R R KPQP P SS 393
Cdd:smart00532 322 IVQ VGRTG K IT PV A E LEP VF L AGST V S R AT L HNEDEIEEK DI RI GD T V V V RK AG DV IP KVVG VV K ek R PG D E R EIEM P TH 401
410 420 430 440
....*....|....*....|....*....|....*....|
gi 2131413628 394 --------- R YN G LTCFYAS - P E C ME Q FFA R LTWLS SR Q A 423
Cdd:smart00532 402 cpscgselv R EE G EVDIRCP n P L C PA Q LIE R IIHFA SR K A 441
LIGANc
cd00114 NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining ...
30-303
6.26e-78
NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor, but using the same basic reaction mechanism. The enzyme reacts with the cofactor to form a phosphoamide-linked AMP with the amino group of a conserved Lysine in the KXDG motif, and subsequently transfers it to the DNA substrate to yield adenylated DNA. This alignment contains members of the NAD+ dependent subfamily only.
Pssm-ID: 238062 [Multi-domain]
Cd Length: 307
Bit Score: 247.89
E-value: 6.26e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 30 QE IA A L KAQ L TRWNED Y WKQGSSE VSD DV YDRL NAR L KQWQRCFHDEPLH D ------- DR P AAS - GT V K HP FAHTGVHKV 101
Cdd:cd00114 1 ER IA E L REL L NKHDYR Y YVLDEPS VSD AE YDRL YRE L RALEEEHPELKTP D sptqrvg GT P LSG f KK V R HP VPMLSLDNA 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 102 ESKQA L SRWMA ------- TQQELW V Q PK V DG VAVT L V Y K NG K L A QA IS RGDG LQ GE EW T AQA R I I PA IP Q TLAG PLANSV 174
Cdd:cd00114 81 FDEEE L RAFDE rikrflg EEPAYV V E PK I DG LSIS L R Y E NG V L V QA AT RGDG TT GE DV T ENV R T I RS IP L TLAG APETLE 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 175 LQ GE L F LLREGHIQQRM -------- GGM N A R AKV AG AMMRATDR - A A LKQTGI FI WAWPN ---- GPK AMKAR L SA L A E A G 241
Cdd:cd00114 161 VR GE V F MPKADFEALNK ereergek PFA N P R NAA AG SLRQLDPK i T A KRPLRF FI YGLGE aegl GPK TQSEA L AF L K E W G 240
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2131413628 242 F TLTARYT L p V KN VAD V E A -- QRTAWFKA SLP FAT DG I VV RASAEPPGEQWLPGEGS -- W VV A W K Y 303
Cdd:cd00114 241 F PVSPETR L - C KN IEE V L A fy DEIEAKRD SLP YEI DG V VV KVDDLALQRELGFTSKA pr W AI A Y K F 305
dnlj
TIGR00575 DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent ...
35-540
3.16e-66
DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent DNA ligases. Functions of these proteins include DNA repair, DNA replication, and DNA recombination. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). The member of this family from Treponema pallidum differs in having three rather than just one copy of the BRCT (BRCA1 C Terminus) domain (pfam00533) at the C-terminus. It is included in the seed. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273148 [Multi-domain]
Cd Length: 652
Bit Score: 227.17
E-value: 3.16e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 35 L KAQLTRWNED Y WKQGSSEV SD DV YDRL NAR L KQWQR c F H D E PLHD D R P --------- AASGT V K H PFAHTGVHKVESKQ 105
Cdd:TIGR00575 1 L RKLIRHHDYR Y YVLDEPSI SD AE YDRL YRE L QELEE - K H P E LITP D S P tqrvgaapl SRFPK V R H STPMLSLDNAFDED 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 106 A L SRW ------- MATQQ E LW V Q PK V DG VA V T L V Y K NG K L AQ A IS RGDG LQ GE EW TA QA R I I PA IP QT LAG PLANSV L Q -- 176
Cdd:TIGR00575 80 E L AAF ikrirrq LGLKV E YV V E PK I DG LS V S L T Y E NG V L VR A LT RGDG TV GE DV TA NV R T I RS IP LR LAG DNPPER L E vr 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 177 GE L F LLR E GH ----- IQQRM G GM --- N A R AKV AG AMMRATD R - A A LKQTGI F IWAWPN G P ---- KAMKAR L SA L AEA GF T 243
Cdd:TIGR00575 160 GE V F MPK E DF ealne ERREQ G EK pfa N P R NAA AG SLRQLDP R i T A KRKLRF F AYGLGE G L elpd ATQYEA L AW L KKW GF P 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 244 LTARYT L p VKNVAD V EAQRTAWFK -- A SLP FAT DG I VV R ----------- ASAEP P geqwlpgeg S W VV A W K YLPVAQV T 310
Cdd:TIGR00575 240 VSPHIR L - CDSIEE V LEYYREIEE kr D SLP YEI DG V VV K vddlalqdelg FTSKA P --------- R W AI A Y K FPAEEAQ T 309
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 311 EVKAIHFT VGRTG R IT AI A Q LEP LMLDDKR V Q R VS L GSVNRWQR LDI AP GD Q V L V SL AG QG IP RLDN V V -- W R NVDR R KP 388
Cdd:TIGR00575 310 KLLDVVVQ VGRTG A IT PV A K LEP VFVAGTT V S R AT L HNEDEIEE LDI RI GD T V V V RK AG DV IP KVVR V L le K R TGSE R PI 389
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 389 QP P SS --------- RYNGLTCFYA - SPE C ME Q FFA R LTWLS SR Q A L DIEG V G ESGWRT L YQA --- HRFEH L FS wlq L TQA 455
Cdd:TIGR00575 390 RF P TH cpscgsplv KIEEEAVIRC p NLN C PA Q RVE R IKHFA SR N A M DIEG L G DKVIEQ L FEK klv RSVAD L YA --- L KKE 466
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 456 Q L TATP G ISASHGAA L WHQFNLAR E R P FI R WIT A M GI P ----- L A RSTLRAA G dr T WQA L SQR S EA E WRMLP GVG QEK A R 530
Cdd:TIGR00575 467 D L LELE G FGEKSAQN L LNAIEKSK E K P LA R LLF A L GI R hvgev T A KNLAKHF G -- T LDK L KAA S LE E LLSVE GVG PKV A E 544
570
....*....|
gi 2131413628 531 QV VN WL H Q P Q 540
Cdd:TIGR00575 545 SI VN FF H D P N 554
Lig
COG0272 NAD-dependent DNA ligase [Replication, recombination and repair];
24-550
4.70e-64
NAD-dependent DNA ligase [Replication, recombination and repair];
Pssm-ID: 440042 [Multi-domain]
Cd Length: 668
Bit Score: 221.44
E-value: 4.70e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 24 S P AK A GQE I AA L KAQ L T R W N ED Y WKQGSS E V SD DV YD R L NAR L KQWQR c F H D E PLHD D R P ------- AAS G -- T V K H P -- 92
Cdd:COG0272 2 T P EE A KER I EE L REE L R R H N YR Y YVLDAP E I SD AE YD A L LRE L QALEA - E H P E LITP D S P tqrvgga PLE G fa K V R H A vp 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 93 ------- F ahtgvhkve S KQA L SRWMA -------- TQQ E LWVQP K V DG V A VT L V Y K NG K L AQ A IS RGDG LQ GE EW TA QA R 157
Cdd:COG0272 81 mlsldna F --------- S EEE L RDFDR rvrkflgd EPV E YVCEL K I DG L A IS L R Y E NG R L VR A AT RGDG TV GE DV TA NV R 151
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 158 I I PA IP QT L A G PLANS VL Q -- GE LFLLREGHIQ qrmgg M N ARAKV AG AMMR A TD R -- AA -- L K Q ----- T ----- GI F IW 221
Cdd:COG0272 152 T I RS IP LR L K G DDVPE VL E vr GE VYMPKADFEA ----- L N EEREE AG EKPF A NP R na AA gs L R Q ldpki T akrpl DF F AY 226
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 222 AW ---- PNG P KAMKAR L SA L A E A GF TLTARYTL p V K NVAD V E A QRTA W FK -- A SLP FAT DG I V V --------------- R 280
Cdd:COG0272 227 GL geve GLL P DTQSEA L EL L K E W GF PVNPERRV - C K SIEE V L A YIEE W EE kr H SLP YEI DG V V I kvndlalqerlgfts R 305
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 281 A sae P pgeqwlpgeg S W VV A W K YLPVAQV T EVKA I HFT VGRTG RI T AI A Q LEP LMLDDKR V Q R VS L GSVNRWQ R L D IAP G 360
Cdd:COG0272 306 A --- P ---------- R W AI A Y K FPAEEAT T KLLD I EVQ VGRTG AL T PV A R LEP VFVAGVT V S R AT L HNEDEIE R K D VRI G 372
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 361 D Q V L V SL AG QG IP RLDN VV wrn VDR R kpq P PSSRY -------------------------- NGL T C F yaspecm E Q FFA R 414
Cdd:COG0272 373 D T V V V RK AG DV IP EVVG VV --- LEK R --- P GDEKP fvmpthcpvcgsplvreegeaalrct NGL S C P ------- A Q LKE R 439
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 415 L TWLS SR Q A L DIEG V GE SGWRT L YQ A --- HRFEH L FS wlq LT QAQ L TATPGI --- SA S hgaalwhqf NL ------ ARER P 482
Cdd:COG0272 440 L KHFA SR K A M DIEG L GE KLIEQ L VD A glv KDPAD L YR --- LT KED L LGLERM gek SA Q --------- NL laaiek SKKT P 507
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2131413628 483 FI R WIT A M GI PL ----- A RSTL R AA G dr TWQ AL SQR SE A E WRMLP G V G QEK A RQV V NWLHQ P Q - IDALAKWL AA 550
Cdd:COG0272 508 LA R FLF A L GI RH vgett A KLLA R HF G -- SLD AL MAA SE E E LAAVD G I G PVV A ESI V EFFAE P H n RELIERLR AA 579
ligA
PRK07956 NAD-dependent DNA ligase LigA; Validated
24-550
1.12e-57
NAD-dependent DNA ligase LigA; Validated
Pssm-ID: 236137 [Multi-domain]
Cd Length: 665
Bit Score: 203.82
E-value: 1.12e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 24 SPAK A GQE I AA L KAQ L TRWNED Y WKQGSSEV SD DV YDRL NAR L KQ -- WQ rcf H D E PLHD D R P ------- AAS G TV K HP fa 94
Cdd:PRK07956 1 TMEE A KKR I EE L REE L NHHAYA Y YVLDAPSI SD AE YDRL YRE L VA le AE --- H P E LITP D S P tqrvgga PLD G FE K VR -- 75
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 95 htgv H -------- KVE S KQA L SRWMA - TQQE L WVQ ------- P K V DG V AV T L V Y K NG K L AQ A IS RGDG LQ GE EW TA QA R I 158
Cdd:PRK07956 76 ---- H lvpmlsld NAF S EEE L RAFDK r VRKR L PDP pltylce L K I DG L AV S L L Y E NG V L VR A AT RGDG TT GE DI TA NV R T 151
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 159 I PA IP QT L A G P l ANSV L -- Q GE L F LLREGHIQ qrmgg M N ARAKVA G AMMR A TD R -- AA -- L K Q -- TG I -------- F IWA 222
Cdd:PRK07956 152 I RS IP LR L H G N - EPER L ev R GE V F MPKADFEA ----- L N EERREE G EKPF A NP R na AA gs L R Q ld PK I takrplsf F AYG 225
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 223 W ----- PNG P KAMKAR L SA L AEA GF t LTAR Y TLPVKNVAD V E A QRTAWFK -- AS LP FAT DG I V VR ----------- AS A E 284
Cdd:PRK07956 226 V geveg GEL P DSQSEA L EF L KAW GF - PVNP Y RKLCTSIEE V L A FYEEIEE er HD LP YDI DG V V IK vddlalqeelg FT A K 304
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 285 P P geqwlpgeg S W VV A W K YLPVAQV T EVKA I HFT VGRTG RI T AI A Q LEP LMLDDKR V Q R VS L GSVNRWQ R L DI AP GD Q V L 364
Cdd:PRK07956 305 A P --------- R W AI A Y K FPAEEAT T KLLD I EVQ VGRTG AV T PV A R LEP VEVAGVT V S R AT L HNADEIE R K DI RI GD T V V 375
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 365 V SL AG QG IP RLDN VV wrn VDR R kpq P PSS R Y -------------------------- NGL T C fyas P ecm E Q FFA RL TWL 418
Cdd:PRK07956 376 V RR AG DV IP EVVG VV --- LEK R --- P GDE R E ivmpthcpvcgselvrvegeavlrct NGL S C ---- P --- A Q LKE RL IHF 442
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 419 S SR Q A L DI E G V GE SGWRT L YQA --- H RFEH LF swl Q LT QAQ L TATP G ISASHGAA L WHQFNLAR E RPFI R WIT A M GI P -- 493
Cdd:PRK07956 443 V SR N A M DI D G L GE KIIEQ L FEK gli H DPAD LF --- K LT AED L LGLE G FGEKSAQN L LDAIEKSK E TSLA R FLY A L GI R hv 519
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2131413628 494 ------- LAR ST lraagd RTWQ AL SQR SE A E WRMLP GVG QEK A RQV V NWLHQPQIDA L - AKW L A A 550
Cdd:PRK07956 520 gekaaka LAR HF ------ GSLE AL RAA SE E E LAAVE GVG EVV A QSI V EFFAVEENRE L i DEL L E A 578
ligA
PRK14351 NAD-dependent DNA ligase LigA; Provisional
24-536
2.13e-42
NAD-dependent DNA ligase LigA; Provisional
Pssm-ID: 184640 [Multi-domain]
Cd Length: 689
Bit Score: 161.46
E-value: 2.13e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 24 S PAK A GQEIAA L KAQLTRWNED Y WKQGSSEVS D DV YD R L N ARL KQWQRC F HDE pl HDDR P AAS --------- G TV K H -- P 92
Cdd:PRK14351 28 S EDE A EEQAEQ L REAIREHDHR Y YVEADPVIA D RA YD A L F ARL QALEDA F DLD -- TENS P TRR vggepldel E TV E H va P 105
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 93 F ---- AHTGVHK V ESKQALS R WMATQQ E LWVQ PK V DG VA V TL VY KN G KLAQ A IS RGDG LQ G EEW TA QA R I I PAI PQ T L A G 168
Cdd:PRK14351 106 M lsid QSGEADD V REFDERV R REVGAV E YVCE PK F DG LS V EV VY ED G EYQR A AT RGDG RE G DDV TA NV R T I RSV PQ K L R G 185
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 169 -- P -- LA nsv LQ GE LFLLREG --- HIQQ R MGG ----- M N A R AKV AG A m M R AT D RA -- A LKQTG IF --- IWAWPNGPKAMK 231
Cdd:PRK14351 186 dy P df LA --- VR GE VYMPKDA fqa YNRE R IER geepf A N P R NAA AG T - L R QL D PS vv A ERPLD IF ffd VLDASELFDSHW 261
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 232 AR L SALA E A G FTL T A R ytlp VKN V A D VEA ----- Q R TAWFKAS L PFAT DG I V V ---- R ASA E PP G EQ wl PGEGS W VV A W K 302
Cdd:PRK14351 262 EE L ERFP E W G LRV T D R ---- TER V D D IDD aiayr D R LLAARDD L NYEI DG V V I kvdd R DAR E EL G AT -- ARAPR W AF A Y K 335
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 303 YLPV A QV T EVKA I HFT VGRTGR I T AI A Q L E P LMLDDKR V Q R V SL GSVNRWQR L DIAP GD Q V L V SL AG QG IP RLDN VV WRN 382
Cdd:PRK14351 336 FPAR A EE T TIRD I VVQ VGRTGR L T PV A L L D P VDVGGVT V S R A SL HNPAEIEE L GVNV GD R V R V KR AG DV IP YVEE VV EKD 415
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 383 ----------------- V D R RK P QPPSS ry N GL T C fyas P ecm E Q FFARLTWLS SR Q ALDIEG V GE SGWRT L YQ A HRF E H 445
Cdd:PRK14351 416 segtfefpdtcpvcdsa V E R DG P LAFCT -- G GL A C ---- P --- A Q LERSIEHYA SR D ALDIEG L GE ERVQQ L VD A GLV E S 486
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 446 L FSWLQ LT Q A Q L TATP G ISASHGAA L WHQFNLA RE R P FIRWIT A M GIP ----- L AR STL R AA G dr T WQ A LSQRS E AEW R M 520
Cdd:PRK14351 487 L ADLYD LT V A D L AELE G WGETSAEN L LAELEAS RE P P LADFLV A L GIP evgpt T AR NLA R EF G -- T FE A IMDAD E EAL R A 564
570
....*....|....*.
gi 2131413628 521 LPG VG QEK A RQVVNWL 536
Cdd:PRK14351 565 VDD VG PTV A EEIREFF 580
DNA_ligase_OB
pfam03120 NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the ...
309-381
7.25e-29
NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This family is a small domain found after the adenylation domain pfam01653 in NAD dependent ligases. OB-fold domains generally are involved in nucleic acid binding.
Pssm-ID: 460813 [Multi-domain]
Cd Length: 79
Bit Score: 109.38
E-value: 7.25e-29
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2131413628 309 V T EVKA I H F T VGRTG R IT AI A Q LEP LM L DDKR V Q R VS L GSVNRWQ R L DI AP GD Q V L V SL AG QG IP RLDN VV WR 381
Cdd:pfam03120 1 E T KLLD I E F Q VGRTG A IT PV A V LEP VE L AGTT V S R AT L HNEDEIK R K DI RI GD T V I V RK AG DV IP EVVG VV LE 73
DNA_ligase_aden
pfam01653 NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining ...
27-305
8.99e-29
NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This domain is the catalytic adenylation domain. The NAD+ group is covalently attached to this domain at the lysine in the KXDG motif of this domain. This enzyme- adenylate intermediate is an important feature of the proposed catalytic mechanism.
Pssm-ID: 396292 [Multi-domain]
Cd Length: 318
Bit Score: 116.29
E-value: 8.99e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 27 K A G Q EIAA L KAQLTRWNED Y WKQGSSE V S D DV YDRL NAR LK QWQR c F H D E PLHD D R P --------- A ASGT V K H PFAHTG 97
Cdd:pfam01653 2 E A Q Q QLEE L RELIRKYDYE Y YVLDNPS V P D AE YDRL YRE LK ALEE - K H P E LITP D S P tqrvgavpl A DFNK V R H LTPMLS 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 98 VHKVESKQA L SRWMATQQELW --------- V Q PK V DG VAVT L V Y K NG K L AQ A IS RGDG LQ GE EW TA QARI I PA IP QT L A G 168
Cdd:pfam01653 81 LDNAFNLDE L QAFIERIRRAL gnkekveyv V E PK I DG LSIS L L Y E NG L L VR A AT RGDG TT GE DV TA NVKT I RN IP LK L K G 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 169 -- P LANSVLQ GE L F LLR E G ----- HIQQRM G G --- M N A R AKV AG A m M R AT D RAALKQTG -- I F IWAWPN ------ G PKAM 230
Cdd:pfam01653 161 dn P PERLEVR GE V F MPK E D fealn EERLEE G E kpf A N P R NAA AG S - L R QL D PRITAKRK lr F F AYGLGL leghel G FDTQ 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 231 KAR L SA L AEA GF TLTARYT L p VKNVAD V E A QRTA W F K -- A SLP FAT DG I VV RASAEPPGEQW lp G EGS ---- W VV A W K YL 304
Cdd:pfam01653 240 YQA L AF L KSL GF PVSPLLA L - CDGIEE V L A YYAD W E K kr D SLP YEI DG V VV KVDELALQREL -- G FTA kapr W AI A Y K FP 316
.
gi 2131413628 305 P 305
Cdd:pfam01653 317 A 317
ligA
PRK14350 NAD-dependent DNA ligase LigA; Provisional
30-492
5.96e-23
NAD-dependent DNA ligase LigA; Provisional
Pssm-ID: 172826 [Multi-domain]
Cd Length: 669
Bit Score: 102.98
E-value: 5.96e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 30 Q EI AA LK AQLTR W NED Y WKQG S SE V S D DV YD RLNA RL KQWQRCFHDEPLH D DRPAAS G T -------- V K H P F AHTGVH KV 101
Cdd:PRK14350 7 D EI LD LK KLIRK W DKE Y YVDS S PS V E D FT YD KALL RL QELESKYPEYKTL D SPTLKF G S dllndfke V E H S F PILSLD KV 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 102 ESKQA L SR W M ---------- ATQQELW V Q PK V DG VAVT L V YK N G K L AQ A IS RGDG LQ G EEW T AQA R I I PAI P QTL a GPLA 171
Cdd:PRK14350 87 YDLKL L KL W I ekmdlensnl GFDFGIS V E PK I DG CSIV L Y YK D G I L EK A LT RGDG RF G NDV T ENV R T I RNV P LFI - DEKV 165
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 172 NS VL Q GE LFLLR E GHIQ ---- QRMGGM NAR AKVA G AMM R ATD R - A A LKQTG IF IWAWPNGPKAM K ARLS A --- L AEA GF T 243
Cdd:PRK14350 166 EL VL R GE IYITK E NFLK inkt LEKPYT NAR NLAS G ILR R IDS R e V A NFPLD IF VYDILYSSLEL K TNHD A fdk L KKF GF K 245
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 244 LT -- A R Y t LPV KN -------- V A D V E AQ R T awfka S LPFAT DG I V VRA S AEPPG E Q -- WLPGEGS W VV A W K YLPVAQVTE 311
Cdd:PRK14350 246 VN pf C R F - FDG KN sieeilny V K D I E KK R N ----- S FEYEI DG V V LKV S DFALR E I lg YTSHHPK W SM A Y K FESLSGFSK 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 312 V KA I HFT VGR T G R IT AI A QL E PLMLDDKRVQRV SL GSVNRWQRLDIAP GD Q V LV S LA G QG IP RLDN V VWR --- NVDRRKP 388
Cdd:PRK14350 320 V ND I VVQ VGR S G K IT PV A NI E KVFVAGAFITNA SL HNQDYIDSIGLNV GD V V KI S RR G DV IP AVEL V IEK lsv GFFKIPD 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2131413628 389 QP PS SRY ---- N G LTC F YASPE C MEQFFA R LTWLS S RQALD I E G VGESGWRT L YQA hrf EHLF S WLQ L TQ --- AQ L TATP 461
Cdd:PRK14350 400 NC PS CKT alik E G AHL F CVNNH C PSVIVE R IKYFC S KKCMN I V G LSDKTIEF L FEK --- KFIS S EID L YT fnf DR L INLK 476
490 500 510
....*....|....*....|....*....|.
gi 2131413628 462 G ISASHGAA L WHQFNLARE RPF IRWITA MGI 492
Cdd:PRK14350 477 G FKDKRINN L KRSIEASKK RPF SKLLLS MGI 507
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
