NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1808313671|gb|KAF2233752|]
View 

VHS-domain-containing protein [Viridothelium virens]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
VHS_GGA_fungi cd16998
VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) ...
 25-163 3.66e-98

VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Yeast GGAs facilitate the specific and direct delivery of vacuolar sorting receptor Vps10p and the processing protease Kex2p from the TGN to the late endosome/prevacuolar compartment (PVC). The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


:

Pssm-ID: 340795 [Multi-domain]  Cd Length: 139  Bit Score: 297.70  E-value: 3.66e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINSKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd16998     1 LQRYIERACDPSLEEPNLALNLEIADLINEKQGNTPREAAFAIVRLVNSRNPTVALLALHLLDVCVKNCGYPFHLQIASK 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1808313671 105 EFLNELVRRFPERPPIRATRAQTKILELIEEWRQTICQTSKYKEDLGFIRDMHRLLSYK 163
Cdd:cd16998    81 EFLNELVRRFPERPPSRLNPVQHKILELIEEWKQTLCKTSRYKEDLGHIRDMHRLLSYK 139
GAT_GGA_fungi cd14235
canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); ...
 234-325 1.05e-36

canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. Two GGAs (Gga1p and Gga2p) have been identified in the budding yeast Saccharomyces cerevisiae. Yeast GGAs play important roles in the carboxypeptidase Y (CPY) pathway, vacuole biogenesis, alpha-factor maturation, and interactions with clathrin. They have a multidomain structure consisting of VHS (Vps27/Hrs/ STAM), GAT (GGA and TOM), hinge, and GAE (gamma-adaptin ear) domains. Both Gga1p and Gga2p function as effectors of Arf in yeast. They interact with Arf1p and Arf2p in a GTP-dependent manner. Moreover, Gga2p mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1, a ferrichrome transporter in S. cerevisiae, from the TGN to the vacuole. It also acts as a phosphatidylinositol 4-phosphate effector at the Golgi exit, which binds directly to the TGN PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. In addition, Gga2p is required for sorting of the yeast siderophore iron transporter1 (Sit1) to the vacuolar pathway. The GAT domain of GGAs interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and/or the tumor susceptibility gene 101 product (TSG101).


:

Pssm-ID: 410583  Cd Length: 92  Bit Score: 132.35  E-value: 1.05e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 234 DYRAKAAEEVLKIQEKARLLEDMLQGHNAGEEIKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRT 313
Cdd:cd14235     1 DYKAQVAEELEKLKRKAILLNEMLNNATPGEIDVEGDTYDELASSLKSAQPKIQKIIEEESEDEESVQKLLELNDLINSL 80

                          90
                  ....*....|..
gi 1808313671 314 IERYKLVKKGDL 325
Cdd:cd14235    81 LERYDLLKKGDA 92
Alpha_adaptinC2 smart00809
Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link ...
 574-677 1.09e-22

Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Gamma-adaptin is a subunit of the golgi adaptor. Alpha adaptin is a heterotetramer that regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This Ig-fold domain is found in alpha, beta and gamma adaptins and consists of a beta-sandwich containing 7 strands in 2 beta-sheets in a greek-key topology.. The adaptor appendage contains an additional N-terminal strand.


:

Pssm-ID: 197886 [Multi-domain]  Cd Length: 104  Bit Score: 93.08  E-value: 1.09e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  574 SSIHVLFHISRQPGpdnVILIQSRISNNTAQPISQLAFQLAVTKGYQLKLEPQSGRALGPHQQhgITQTIRLHGVGPGQg 653
Cdd:smart00809   5 NGLQIGFKFERRPG---LIRITLTFTNKSPSPITNFSFQAAVPKSLKLQLQPPSSPTLPPGGQ--ITQVLKVENPGKFP- 78

                           90       100
                   ....*....|....*....|....
gi 1808313671  654 ssVKMRWKASYDVGTEHKEEMGDI 677
Cdd:smart00809  79 --LRLRLRLSYLLGGSAVTEQGDV 100
GGA_N-GAT super family cl39655
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 198-230 9.23e-04

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


The actual alignment was detected with superfamily member pfam18308:

Pssm-ID: 465702  Cd Length: 39  Bit Score: 37.28  E-value: 9.23e-04
                          10        20        30
                  ....*....|....*....|....*....|...
gi 1808313671 198 QSAKLQELIRRGSPHDLQEANKLMKVMAGYDNR 230
Cdd:pfam18308   6 KSKLLARLLKSKNPEDLQAANRLIKSMVKEDDE 38
PRK12323 super family cl46901
DNA polymerase III subunit gamma/tau;
410-505 3.22e-03

DNA polymerase III subunit gamma/tau;


The actual alignment was detected with superfamily member PRK14971:

Pssm-ID: 481241 [Multi-domain]  Cd Length: 614  Bit Score: 40.53  E-value: 3.22e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 410 SDGLGNSGLPQPAQSTQPTIS-SMNQSNLSQQVAPS-QPSTQTKPNYDPFGPISNAQTTSKPATPTPSLGS---PPPQHQ 484
Cdd:PRK14971  364 QKGDDASGGRGPKQHIKPVFTqPAAAPQPSAAAAASpSPSQSSAAAQPSAPQSATQPAGTPPTVSVDPPAAvpvNPPSTA 443

                          90       100
                  ....*....|....*....|.
gi 1808313671 485 AQQTRIDPFASLNAPAPRQAS 505
Cdd:PRK14971  444 PQAVRPAQFKEEKKIPVSKVS 464
 
Name Accession Description Interval E-value
VHS_GGA_fungi cd16998
VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) ...
 25-163 3.66e-98

VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Yeast GGAs facilitate the specific and direct delivery of vacuolar sorting receptor Vps10p and the processing protease Kex2p from the TGN to the late endosome/prevacuolar compartment (PVC). The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340795 [Multi-domain]  Cd Length: 139  Bit Score: 297.70  E-value: 3.66e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINSKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd16998     1 LQRYIERACDPSLEEPNLALNLEIADLINEKQGNTPREAAFAIVRLVNSRNPTVALLALHLLDVCVKNCGYPFHLQIASK 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1808313671 105 EFLNELVRRFPERPPIRATRAQTKILELIEEWRQTICQTSKYKEDLGFIRDMHRLLSYK 163
Cdd:cd16998    81 EFLNELVRRFPERPPSRLNPVQHKILELIEEWKQTLCKTSRYKEDLGHIRDMHRLLSYK 139
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 24-164 1.56e-57

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 190.97  E-value: 1.56e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKkGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQIST 103
Cdd:pfam00790   1 PLERWIDKATDPSLREEDWSLILDICDLINET-ETGPKEAVRLIKKRINSPNPHVALLALTLLDACVKNCGSKFHLEIAS 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1808313671 104 KEFLNELVRRFPERPPIRATRAQTKILELIEEWRQTICQtskyKEDLGFIRDMHRLLSYKG 164
Cdd:pfam00790  80 KEFLNELVKLIPNKPYGKPAKVKKKILELIQEWADAFKN----DPDLKYIRDMYKLLKRKG 136
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 25-164 1.80e-50

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 171.71  E-value: 1.80e-50
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671   25 LQRYIQNACDPTNFEPNLALNLEITDLINSKkGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:smart00288   1 VERLIDKATSPSLLEEDWELILEICDLINST-PDGPKDAVRLLKKRLNNKNPHVALLALTLLDACVKNCGSKFHLEVASK 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  105 EFLNELVRRFPERPPIRatRAQTKILELIEEWRQTIcqtsKYKEDLGFIRDMHRLLSYKG 164
Cdd:smart00288  80 EFLNELVKLIKPKYPLP--LVKKRILELIQEWADAF----KNDPDLSQIVDVYDLLKKKG 133
GAT_GGA_fungi cd14235
canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); ...
 234-325 1.05e-36

canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. Two GGAs (Gga1p and Gga2p) have been identified in the budding yeast Saccharomyces cerevisiae. Yeast GGAs play important roles in the carboxypeptidase Y (CPY) pathway, vacuole biogenesis, alpha-factor maturation, and interactions with clathrin. They have a multidomain structure consisting of VHS (Vps27/Hrs/ STAM), GAT (GGA and TOM), hinge, and GAE (gamma-adaptin ear) domains. Both Gga1p and Gga2p function as effectors of Arf in yeast. They interact with Arf1p and Arf2p in a GTP-dependent manner. Moreover, Gga2p mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1, a ferrichrome transporter in S. cerevisiae, from the TGN to the vacuole. It also acts as a phosphatidylinositol 4-phosphate effector at the Golgi exit, which binds directly to the TGN PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. In addition, Gga2p is required for sorting of the yeast siderophore iron transporter1 (Sit1) to the vacuolar pathway. The GAT domain of GGAs interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and/or the tumor susceptibility gene 101 product (TSG101).


Pssm-ID: 410583  Cd Length: 92  Bit Score: 132.35  E-value: 1.05e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 234 DYRAKAAEEVLKIQEKARLLEDMLQGHNAGEEIKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRT 313
Cdd:cd14235     1 DYKAQVAEELEKLKRKAILLNEMLNNATPGEIDVEGDTYDELASSLKSAQPKIQKIIEEESEDEESVQKLLELNDLINSL 80

                          90
                  ....*....|..
gi 1808313671 314 IERYKLVKKGDL 325
Cdd:cd14235    81 LERYDLLKKGDA 92
Alpha_adaptinC2 smart00809
Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link ...
 574-677 1.09e-22

Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Gamma-adaptin is a subunit of the golgi adaptor. Alpha adaptin is a heterotetramer that regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This Ig-fold domain is found in alpha, beta and gamma adaptins and consists of a beta-sandwich containing 7 strands in 2 beta-sheets in a greek-key topology.. The adaptor appendage contains an additional N-terminal strand.


Pssm-ID: 197886 [Multi-domain]  Cd Length: 104  Bit Score: 93.08  E-value: 1.09e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  574 SSIHVLFHISRQPGpdnVILIQSRISNNTAQPISQLAFQLAVTKGYQLKLEPQSGRALGPHQQhgITQTIRLHGVGPGQg 653
Cdd:smart00809   5 NGLQIGFKFERRPG---LIRITLTFTNKSPSPITNFSFQAAVPKSLKLQLQPPSSPTLPPGGQ--ITQVLKVENPGKFP- 78

                           90       100
                   ....*....|....*....|....
gi 1808313671  654 ssVKMRWKASYDVGTEHKEEMGDI 677
Cdd:smart00809  79 --LRLRLRLSYLLGGSAVTEQGDV 100
GAT pfam03127
GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of ...
 247-323 7.85e-22

GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of the ARF family including ARF1 and ARF3. The GAT domain stabilizes membrane bound ARF1 in its GTP bound state, by interfering with GAP proteins.


Pssm-ID: 460818  Cd Length: 76  Bit Score: 89.52  E-value: 7.85e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1808313671 247 QEKARLLEDMLQGHNAGEEiKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRTIERYKLVKKG 323
Cdd:pfam03127   1 RNNAKLLNEMLSSTDPDEE-LDNELIKELYERCKSAQPKIQKLIEETSDEDDLLAELLQLNDDLNNVLERYERLKKG 76
Alpha_adaptinC2 pfam02883
Adaptin C-terminal domain; Alpha adaptin is a heterotetramer which regulates clathrin-bud ...
 574-673 3.20e-18

Adaptin C-terminal domain; Alpha adaptin is a heterotetramer which regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This ig-fold domain is found in alpha, beta and gamma adaptins.


Pssm-ID: 460735  Cd Length: 111  Bit Score: 80.45  E-value: 3.20e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 574 SSIHVLFHISRQPGPdNVILIQSRISNNTAQPISQLAFQLAVTKGYQLKLEPQSGRALGPHQQHGITQTIRLHGVGPGQg 653
Cdd:pfam02883   9 DGLQIGFSFERSRRP-GQIRITLTFTNKSSSPISNFSFQAAVPKSLKLQLQPPSSNVLPPNPGGQITQVLLIENPGKKP- 86

                          90       100
                  ....*....|....*....|
gi 1808313671 654 ssVKMRWKASYDVGTEHKEE 673
Cdd:pfam02883  87 --LRMRLKISYLNGGAVQEQ 104
GGA_N-GAT pfam18308
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 198-230 9.23e-04

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


Pssm-ID: 465702  Cd Length: 39  Bit Score: 37.28  E-value: 9.23e-04
                          10        20        30
                  ....*....|....*....|....*....|...
gi 1808313671 198 QSAKLQELIRRGSPHDLQEANKLMKVMAGYDNR 230
Cdd:pfam18308   6 KSKLLARLLKSKNPEDLQAANRLIKSMVKEDDE 38
PRK14971 PRK14971
DNA polymerase III subunit gamma/tau;
410-505 3.22e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 237874 [Multi-domain]  Cd Length: 614  Bit Score: 40.53  E-value: 3.22e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 410 SDGLGNSGLPQPAQSTQPTIS-SMNQSNLSQQVAPS-QPSTQTKPNYDPFGPISNAQTTSKPATPTPSLGS---PPPQHQ 484
Cdd:PRK14971  364 QKGDDASGGRGPKQHIKPVFTqPAAAPQPSAAAAASpSPSQSSAAAQPSAPQSATQPAGTPPTVSVDPPAAvpvNPPSTA 443

                          90       100
                  ....*....|....*....|.
gi 1808313671 485 AQQTRIDPFASLNAPAPRQAS 505
Cdd:PRK14971  444 PQAVRPAQFKEEKKIPVSKVS 464
 
Name Accession Description Interval E-value
VHS_GGA_fungi cd16998
VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) ...
 25-163 3.66e-98

VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Yeast GGAs facilitate the specific and direct delivery of vacuolar sorting receptor Vps10p and the processing protease Kex2p from the TGN to the late endosome/prevacuolar compartment (PVC). The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340795 [Multi-domain]  Cd Length: 139  Bit Score: 297.70  E-value: 3.66e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINSKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd16998     1 LQRYIERACDPSLEEPNLALNLEIADLINEKQGNTPREAAFAIVRLVNSRNPTVALLALHLLDVCVKNCGYPFHLQIASK 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1808313671 105 EFLNELVRRFPERPPIRATRAQTKILELIEEWRQTICQTSKYKEDLGFIRDMHRLLSYK 163
Cdd:cd16998    81 EFLNELVRRFPERPPSRLNPVQHKILELIEEWKQTLCKTSRYKEDLGHIRDMHRLLSYK 139
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 24-164 1.56e-57

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 190.97  E-value: 1.56e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKkGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQIST 103
Cdd:pfam00790   1 PLERWIDKATDPSLREEDWSLILDICDLINET-ETGPKEAVRLIKKRINSPNPHVALLALTLLDACVKNCGSKFHLEIAS 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1808313671 104 KEFLNELVRRFPERPPIRATRAQTKILELIEEWRQTICQtskyKEDLGFIRDMHRLLSYKG 164
Cdd:pfam00790  80 KEFLNELVKLIPNKPYGKPAKVKKKILELIQEWADAFKN----DPDLKYIRDMYKLLKRKG 136
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 25-164 1.80e-50

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 171.71  E-value: 1.80e-50
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671   25 LQRYIQNACDPTNFEPNLALNLEITDLINSKkGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:smart00288   1 VERLIDKATSPSLLEEDWELILEICDLINST-PDGPKDAVRLLKKRLNNKNPHVALLALTLLDACVKNCGSKFHLEVASK 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  105 EFLNELVRRFPERPPIRatRAQTKILELIEEWRQTIcqtsKYKEDLGFIRDMHRLLSYKG 164
Cdd:smart00288  80 EFLNELVKLIKPKYPLP--LVKKRILELIQEWADAF----KNDPDLSQIVDVYDLLKKKG 133
VHS_GGA cd16977
VHS (Vps27/Hrs/STAM) domain of GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) ...
 25-163 1.39e-44

VHS (Vps27/Hrs/STAM) domain of GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) subfamily; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340774  Cd Length: 133  Bit Score: 155.81  E-value: 1.39e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINsKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd16977     1 LERWINRATNPSNAEPDWALILGFCDQIN-EDGEGPRDAARLLAHKIQSPQEWEAIQALTVLEVCMKNCGYRFHLEVGKF 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1808313671 105 EFLNELVRRFPERPPI--RATRAQTKILELIEEWRQTICQtskykedLGFIRDMHRLLSYK 163
Cdd:cd16977    80 RFLNELIKVVSPKYLGsrRSEKVKRKILELLYSWTVTLPE-------EGKIRDAYQMLKKQ 133
GAT_GGA_fungi cd14235
canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); ...
 234-325 1.05e-36

canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. Two GGAs (Gga1p and Gga2p) have been identified in the budding yeast Saccharomyces cerevisiae. Yeast GGAs play important roles in the carboxypeptidase Y (CPY) pathway, vacuole biogenesis, alpha-factor maturation, and interactions with clathrin. They have a multidomain structure consisting of VHS (Vps27/Hrs/ STAM), GAT (GGA and TOM), hinge, and GAE (gamma-adaptin ear) domains. Both Gga1p and Gga2p function as effectors of Arf in yeast. They interact with Arf1p and Arf2p in a GTP-dependent manner. Moreover, Gga2p mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1, a ferrichrome transporter in S. cerevisiae, from the TGN to the vacuole. It also acts as a phosphatidylinositol 4-phosphate effector at the Golgi exit, which binds directly to the TGN PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. In addition, Gga2p is required for sorting of the yeast siderophore iron transporter1 (Sit1) to the vacuolar pathway. The GAT domain of GGAs interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and/or the tumor susceptibility gene 101 product (TSG101).


Pssm-ID: 410583  Cd Length: 92  Bit Score: 132.35  E-value: 1.05e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 234 DYRAKAAEEVLKIQEKARLLEDMLQGHNAGEEIKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRT 313
Cdd:cd14235     1 DYKAQVAEELEKLKRKAILLNEMLNNATPGEIDVEGDTYDELASSLKSAQPKIQKIIEEESEDEESVQKLLELNDLINSL 80

                          90
                  ....*....|..
gi 1808313671 314 IERYKLVKKGDL 325
Cdd:cd14235    81 LERYDLLKKGDA 92
VHS cd03561
VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein ...
 29-146 7.08e-34

VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It has a superhelical structure similar to that of the ARM (Armadillo) repeats and is present at the N-termini of proteins involved in intracellular membrane trafficking. There are four general groups of VHS domain containing proteins based on their association with other domains. The first group consists of proteins of the STAM/EAST/Hbp family, which has the domain composition of VHS-SH3-ITAM. The second consists of proteins with a FYVE domain C-terminal to VHS. The third consists of GGA proteins with a domain composition of VHS-GAT (GGA and TOM)-GAE (Gamma-Adaptin Ear) domain. The fourth consists of proteins with a VHS domain alone or with domains other than those mentioned above. In GGA proteins, VHS domains are involved in cargo recognition in trans-Golgi, thereby having a general membrane targeting/cargo recognition role in vesicular trafficking.


Pssm-ID: 340765  Cd Length: 131  Bit Score: 125.84  E-value: 7.08e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  29 IQNACDPTNFEPNLALNLEITDLINSKKGNApREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEFLN 108
Cdd:cd03561     5 VEKATSESLTEPDWALNLEICDLVNSDPAQA-KDAVRALRKRLQSKNPKVQLLALTLLETLVKNCGAPFHSEVASRDFLQ 83

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1808313671 109 ELVRRFpeRPPIRATRAQTKILELIEEWRQTICQTSKY 146
Cdd:cd03561    84 ELVKLV--KKKKTSPEVREKALALIQEWAEAFQGDGRL 119
VHS_Hrs cd03569
VHS (Vps27/Hrs/STAM) domain of Hepatocyte growth factor-regulated tyrosine kinase substrate, ...
 23-168 5.06e-26

VHS (Vps27/Hrs/STAM) domain of Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs; Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) plays a role in at least three vesicle trafficking events: exocytosis, endocytosis, and endosome to lysosome trafficking. Hrs is involved in promoting rapid recycling of endocytosed signaling receptors to the plasma membrane. Together with STAM or STAM2, it comprises the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery, which functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles. Hrs contains an N-terminal VHS domain, which has a superhelical structure similar to the structure of ARM (Armadillo) repeats, a FYVE (Fab1p, YOTB, Vac1p, and EEA1) zinc finger domain, a Double Ubiquitin-Interacting Motif (DUIM), a P(S/T)XP motif that recruit ESCRT-I, a GAT (GGA and TOM) domain, and a short peptide motif near the C-terminus that recruits clathrin.


Pssm-ID: 340771  Cd Length: 138  Bit Score: 103.87  E-value: 5.06e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  23 TPLQRYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQIS 102
Cdd:cd03569     1 SNFDKLLDKATSQLLLEPDWESILQICDLIRQGD-VTPKYAVSAIKKKLNDQNPHVALYALQVLESVVKNCGTPVHDEVA 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1808313671 103 TKEFLN---ELVRRFPErPPIRatraqTKILELIEEWRQTICQTSKYKedlgFIRDMHRLLSYKGYMFP 168
Cdd:cd03569    80 TKEFMEelrDLVKTTTS-ENVR-----NKILELIQAWAHAFRNEPKYK----IVQDTYNIMKMEGYKFP 138
VHS_Vps27 cd16979
VHS (Vps27/Hrs/STAM) domain of Vacuolar protein sorting-associated protein 27; Vacuolar ...
 23-168 5.84e-26

VHS (Vps27/Hrs/STAM) domain of Vacuolar protein sorting-associated protein 27; Vacuolar protein sorting-associated protein 27 (Vps27 or Vps27p) is also called Golgi retention defective protein 11, and is the yeast homolog of Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate). Together with class E vacuolar protein-sorting machinery protein HSE1, it comprises the ESCRT-0 complex, the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB). The complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. It is also required the efficient recycling of late Golgi proteins including the carboxypeptidase Y (CPY) sorting receptor, Vps10. Vps27 contain similar domains and motifs to Hrs; it contains an N-terminal VHS domain, which has a superhelical structure similar to the structure of ARM (Armadillo) repeats, a FYVE (Fab1p, YOTB, Vac1p, and EEA1) zinc finger domain, two Ubiquitin-Interacting Motifs (UIMs), a GAT (GGA and TOM) domain, two a P(S/T)XP motifs that recruit ESCRT-I, and a short peptide motif near the C-terminus that recruits clathrin.


Pssm-ID: 340776  Cd Length: 141  Bit Score: 103.81  E-value: 5.84e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  23 TPLQRYIQNACDPT--NFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQ 100
Cdd:cd16979     1 SEFDELIEKATSESipNGEEDLALALEISDLIRSKS-VPPKDAMRSLKKRILHKNPNVQLLALKLTDVCVKNGGDHFLVE 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1808313671 101 ISTKEFLNELVRRfperppIRATRA----QTKILELIEEWrqtiCQTSKYKEDLGFIRDMHRLLSYKGYMFP 168
Cdd:cd16979    80 IASREFMDNLVSL------IRSTGVnpevKEKILRLIQEW----ALAFKSKSQLSYVVEVYKKLKSEGIEFP 141
VHS_STAM cd03568
VHS (Vps27/Hrs/STAM) domain of the STAM (Signal Transducing Adaptor Molecule) subfamily; STAM ...
 29-143 6.16e-25

VHS (Vps27/Hrs/STAM) domain of the STAM (Signal Transducing Adaptor Molecule) subfamily; STAM (Signal Transducing Adaptor Molecule) subfamily members have at their N-termini a VHS domain, which is involved in cytokine-mediated intracellular signal transduction and has a superhelical structure similar to the structure of ARM (Armadillo) repeats, followed by a Ubiquitin-Interacting Motif (UIM) and a SH3 (Src Homology 3) domain, which is a well-established protein-protein interaction domain, and a GAT (GGA and TOM) domain. At the C-termini of most vertebrate STAMs, an Immunoreceptor Tyrosine-based Activation Motif (ITAM) is present, which mediates the binding of HRS (hepatocyte growth factor-regulated tyrosine kinase substrate) in endocytic and exocytic machineries. STAM is a component of the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery and together with Hrs, functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles. Jawed vertebrates have two STAM subfamily members, STAM1 and STAM2.


Pssm-ID: 340770  Cd Length: 132  Bit Score: 100.44  E-value: 6.16e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  29 IQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEFLN 108
Cdd:cd03568     5 VEKATSEKNTSENWSLILDICDKVGRSP-NGAKDCLKSIMKRLNHKDPHVALQALTLLDACVKNCGKIFHLEVCSRDFES 83

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1808313671 109 ELVRRFPERPPIRAtraqTKILELIEEWRQTICQT 143
Cdd:cd03568    84 ELRKLLTKSHPKVA----EKLKELLKKWAEEEFKD 114
VHS_Tom1_like cd03565
VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily; This subfamily is composed of Tom1 (Target of ...
 24-136 2.24e-23

VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily; This subfamily is composed of Tom1 (Target of myb1 - retroviral oncogene) protein, Tom1L1 (Tom1-like1), Tom1L2 (Tom1-like2), and similar proteins. Proteins belonging to this subfamily are characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. They are novel regulators for post-Golgi trafficking and signaling. Yeast do not contain homologous proteins of the Tom1 subfamily, suggesting these proteins have evolved to accommodate more complex cellular processes. Tom1 is essential for the negative regulation of Interleukin-1 and Tumor Necrosis Factor-induced signaling pathways. The VHS domain has a superhelical structure similar to the structure of the ARM repeats and is present at the very N-termini of proteins. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340768  Cd Length: 138  Bit Score: 96.18  E-value: 2.24e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVN-LINSRNPN--VSMLALTLLDICVKNCGYPFHLQ 100
Cdd:cd03565     1 PVGQLIERATDSGLSSEDWALNMEICDMINETD-DGPKDAIKAIKKrLSTNAGKNhtVVMYTLTLLETCVKNCGHRFHVL 79

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1808313671 101 ISTKEFLNELVRRF-PERPPirATRAQTKILELIEEW 136
Cdd:cd03565    80 VANKDFLQELVKLIgPKNDP--PTIVQEKVLSLIQSW 114
Alpha_adaptinC2 smart00809
Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link ...
 574-677 1.09e-22

Adaptin C-terminal domain; Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Gamma-adaptin is a subunit of the golgi adaptor. Alpha adaptin is a heterotetramer that regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This Ig-fold domain is found in alpha, beta and gamma adaptins and consists of a beta-sandwich containing 7 strands in 2 beta-sheets in a greek-key topology.. The adaptor appendage contains an additional N-terminal strand.


Pssm-ID: 197886 [Multi-domain]  Cd Length: 104  Bit Score: 93.08  E-value: 1.09e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  574 SSIHVLFHISRQPGpdnVILIQSRISNNTAQPISQLAFQLAVTKGYQLKLEPQSGRALGPHQQhgITQTIRLHGVGPGQg 653
Cdd:smart00809   5 NGLQIGFKFERRPG---LIRITLTFTNKSPSPITNFSFQAAVPKSLKLQLQPPSSPTLPPGGQ--ITQVLKVENPGKFP- 78

                           90       100
                   ....*....|....*....|....
gi 1808313671  654 ssVKMRWKASYDVGTEHKEEMGDI 677
Cdd:smart00809  79 --LRLRLRLSYLLGGSAVTEQGDV 100
GAT pfam03127
GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of ...
 247-323 7.85e-22

GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of the ARF family including ARF1 and ARF3. The GAT domain stabilizes membrane bound ARF1 in its GTP bound state, by interfering with GAP proteins.


Pssm-ID: 460818  Cd Length: 76  Bit Score: 89.52  E-value: 7.85e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1808313671 247 QEKARLLEDMLQGHNAGEEiKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRTIERYKLVKKG 323
Cdd:pfam03127   1 RNNAKLLNEMLSSTDPDEE-LDNELIKELYERCKSAQPKIQKLIEETSDEDDLLAELLQLNDDLNNVLERYERLKKG 76
VHS_ENTH_ANTH cd00197
VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a ...
 27-137 2.07e-19

VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a VHS, CID, ENTH, or ANTH domain. The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It is located at the N-termini of proteins involved in intracellular membrane trafficking. The CTD-Interacting Domain (CID) is present in several RNA-processing factors and binds tightly to the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP II or Pol II). The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. A set of proteins previously designated as harboring an ENTH domain in fact contains a highly similar, yet unique module referred to as an AP180 N-Terminal Homology (ANTH) domain. VHS, ENTH, and ANTH domains are structurally similar and are composed of a superhelix of eight alpha helices. ENTH and ANTH (E/ANTH) domains bind both inositol phospholipids and proteins and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. E/ANTH domain-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340764  Cd Length: 115  Bit Score: 84.01  E-value: 2.07e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  27 RYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEF 106
Cdd:cd00197     3 KTVEKATSNENMGPDWPLIMEICDLINETN-VGPKEAVDAIKKRINNKNPHVVLKALTLLEYCVKNCGERFHQEVASNDF 81

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1808313671 107 LNELvrRFPERPPIRATRA----QTKILELIEEWR 137
Cdd:cd00197    82 AVEL--LKFDKSGLLGDDVstnvREKAIELVQLWA 114
VHS_HSE1 cd16978
VHS (Vps27/Hrs/STAM) domain of Class E vacuolar protein-sorting machinery protein HSE1; Class ...
 29-155 2.71e-18

VHS (Vps27/Hrs/STAM) domain of Class E vacuolar protein-sorting machinery protein HSE1; Class E vacuolar protein-sorting machinery protein HSE1, together with Vps27, comprise the ESCRT-0 complex, the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB). The complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. It is also required the efficient recycling of late Golgi proteins including the carboxypeptidase Y (CPY) sorting receptor, Vps10. Similar to metazoan STAMs, HSE1 contain: an N-terminal VHS domain, which is involved in cytokine-mediated intracellular signal transduction and has a superhelical structure similar to the structure of ARM (Armadillo) repeats; a Ubiquitin-Interacting Motif (UIM); a SH3 (Src Homology 3) domain, a well-established protein-protein interaction domain; and a GAT (GGA and TOM) domain, which is essential for the normal sorting function of HSE1.


Pssm-ID: 340775  Cd Length: 134  Bit Score: 81.46  E-value: 2.71e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  29 IQNACDPTNFEPNLALNLEITDLINSKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEFLN 108
Cdd:cd16978     5 IIKATDETLTSENWQYILDVCDKVNSDPEDGAREAIAAIQKRLQHRNANVQLRSLSLADALAKNCGSRLHQEISSRSFTQ 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1808313671 109 ELVRRFPErppiRAT--RAQTKILELIEEWRQTIcqtsKYKEDLGFIRD 155
Cdd:cd16978    85 TLLRLIND----RTThaTVKKRILELIKQLSDSF----KSDPSLGLMSD 125
Alpha_adaptinC2 pfam02883
Adaptin C-terminal domain; Alpha adaptin is a heterotetramer which regulates clathrin-bud ...
 574-673 3.20e-18

Adaptin C-terminal domain; Alpha adaptin is a heterotetramer which regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This ig-fold domain is found in alpha, beta and gamma adaptins.


Pssm-ID: 460735  Cd Length: 111  Bit Score: 80.45  E-value: 3.20e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 574 SSIHVLFHISRQPGPdNVILIQSRISNNTAQPISQLAFQLAVTKGYQLKLEPQSGRALGPHQQHGITQTIRLHGVGPGQg 653
Cdd:pfam02883   9 DGLQIGFSFERSRRP-GQIRITLTFTNKSSSPISNFSFQAAVPKSLKLQLQPPSSNVLPPNPGGQITQVLLIENPGKKP- 86

                          90       100
                  ....*....|....*....|
gi 1808313671 654 ssVKMRWKASYDVGTEHKEE 673
Cdd:pfam02883  87 --LRMRLKISYLNGGAVQEQ 104
VHS_GGA_metazoan cd03567
VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, ...
 24-148 4.53e-18

VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Jawed vertebrates contain as many as three GGA proteins: GGA1, GGA2, and GGA3. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340769  Cd Length: 139  Bit Score: 81.10  E-value: 4.53e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQIST 103
Cdd:cd03567     1 SLEYLLNKATNPFNTEEDWEAIDAFCDRVNKEP-EGPQIAVRLLAHKIQSPNEKEALRALTVLEACVKNCGPRFHSEVGK 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1808313671 104 KEFLNELVR-----RFPERPPIratRAQTKILELIEEWRQTICQTSKYKE 148
Cdd:cd03567    80 FRFLNELIKlvspkYLGNRTPE---EVKQKIIELLYSWTRSLPHEPKIKE 126
VHS_Tom1L2 cd16996
VHS (Vps27/Hrs/STAM) domain of TOM1-like protein 2; TOM1-like protein 2 (Tom1L2) is a member ...
 24-136 2.67e-16

VHS (Vps27/Hrs/STAM) domain of TOM1-like protein 2; TOM1-like protein 2 (Tom1L2) is a member of the Tom1 (Target of myb1) subfamily, characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. They are novel regulators for post-Golgi trafficking and signaling. Studies in Tom1L2 hypomorphic mice suggest that Tom1L2 may play roles in immune responses and tumor suppression. The VHS domain has a superhelical structure similar to the structure of the ARM repeats and is present at the very N-termini of proteins. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340793 [Multi-domain]  Cd Length: 137  Bit Score: 76.23  E-value: 2.67e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLIN-SRNPNVSMLALTLLDICVKNCGYPFHLQIS 102
Cdd:cd16996     1 PVGQCIEKATDGSLQSEDWTLNMEICDIINETE-EGPKDAIRALKKRLNgNKNYREVMLALTVLETCVKNCGHRFHVLVA 79

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1808313671 103 TKEFLNELVRRF--PERPPirATRAQTKILELIEEW 136
Cdd:cd16996    80 SRDFIDGVLVKIisPKNNP--PTIVQDKVLALIQAW 113
VHS_Tom1 cd16995
VHS (Vps27/Hrs/STAM) domain of Target of Myb protein 1; Tom1 (Target of myb1 - retroviral ...
 24-136 3.65e-16

VHS (Vps27/Hrs/STAM) domain of Target of Myb protein 1; Tom1 (Target of myb1 - retroviral oncogene) is a novel negative regulator of interleukin-1 and tumor necrosis factor-induced signaling pathways. It also plays important roles in protein-degradation systems in Alzheimer's disease pathogenesis. Tom1 contains VHS and GAT domains in the N-terminal and central region, respectively. The VHS domain has a superhelical structure similar to the structure of the ARM repeats and is present at the very N-termini of proteins. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting. The VHS domain of Tom1 is essential for its function as a negative regulator.


Pssm-ID: 340792  Cd Length: 137  Bit Score: 75.72  E-value: 3.65e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  24 PLQRYIQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVN-LINSRNPNVSMLALTLLDICVKNCGYPFHLQIS 102
Cdd:cd16995     1 PVGQRIEKATDGSLQSEDWGLNMEICDIINETE-EGPKDAIRAIKKrIVGNKNFREVMLALTVLETCVKNCGHRFHVLVA 79

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1808313671 103 TKEFL-NELVRR-FPERPPirATRAQTKILELIEEW 136
Cdd:cd16995    80 SQDFVeGVLVKTiLPKNNP--PAIVHDKVLNLIQAW 113
VHS_STAM2 cd16999
VHS (Vps27/Hrs/STAM) domain of Signal Transducing Adapter Molecule 2; Signal Transducing ...
 29-138 1.94e-12

VHS (Vps27/Hrs/STAM) domain of Signal Transducing Adapter Molecule 2; Signal Transducing Adapter Molecule 2 (STAM2) is also called EAST (EGFR-Associated protein with SH3 and TAM domain) and Hbp (Hrs-binding protein). It is highly expressed in neurons, where it is localized in the nucleus. STAM (Signal Transducing Adaptor Molecule) subfamily members have at their N-termini a VHS domain, which is involved in cytokine-mediated intracellular signal transduction and has a superhelical structure similar to the structure of ARM (Armadillo) repeats, followed by a Ubiquitin-Interacting Motif (UIM) and a SH3 (Src Homology 3) domain, which is a well-established protein-protein interaction domain, and a GAT (GGA and TOM) domain. At the C-termini of most vertebrate STAMS, an Immunoreceptor Tyrosine-based Activation Motif (ITAM) is present, which mediates the binding of HRS (hepatocyte growth factor-regulated tyrosine kinase substrate) in endocytic and exocytic machineries. STAM is a component of the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery and together with Hrs, functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles.


Pssm-ID: 340796 [Multi-domain]  Cd Length: 139  Bit Score: 64.99  E-value: 1.94e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  29 IQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEFLN 108
Cdd:cd16999     5 VEKATNEYNTSEDWSLIMDICDKVGSTP-NGAKDCLKAIMKRVNHKVPHVALQALTLLGACVSNCGKIFHLEVCSRDFAT 83

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1808313671 109 ElVRRfperppIRATRAQTKILE----LIEEWRQ 138
Cdd:cd16999    84 E-VRA------VIKNKAHPKVCEklkaLMVEWSE 110
VHS_Lsb5 cd16980
VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein ...
 27-159 2.84e-12

VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein 5 (LAS17-binding protein 5, Lsb5, or Lsb5p) localizes to the plasma membrane and plays a role in endocytosis in yeast. It interacts with actin regulators Sla1p and Las17p, ubiquitin, and Arf3p, coupling actin dynamics to membrane trafficking processes. Lsb5p contains an N-terminal VHS domain and a GAT (GGA and TOM) domain. The VHS domain has a superhelical structure similar to the structure of ARM (Armadillo) repeats. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340777  Cd Length: 132  Bit Score: 64.16  E-value: 2.84e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  27 RYIQNACDPTNFEPNLALNLEITDLINSKkGNAPREAAVAIvnlinsR------NPNVSMLALTLLDICVKNCGYPFHLQ 100
Cdd:cd16980     3 DTIERLTSSEYEEEDWSLVLELCELINLI-ESGAKEAARAL------RkklkygNPHQQLRALTLLDLLVENGGKKFQAQ 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1808313671 101 ISTKEFLNELVR----RFPERPPIRAtraqtKILELIEEWrqticqTSKYKEDLGFiRDMHRL 159
Cdd:cd16980    76 FASDKKLEDLLRliasDPSTDPKVRK-----KLLELLGGW------AYEFKGDPGL-SGIASL 126
VHS_STAM1 cd17000
VHS (Vps27/Hrs/STAM) domain of Signal Transducing Adapter Molecule 1; Signal Transducing ...
 29-137 2.31e-11

VHS (Vps27/Hrs/STAM) domain of Signal Transducing Adapter Molecule 1; Signal Transducing Adapter Molecule 1 (STAM1) is part of a crucial regulatory axis for the ventral axonal trajectory of developing spinal motor neurons. It forms a complex with beta-arrestin, which regulates lysosomal trafficking of the chemokine receptor CXCR4 and also mediates CXCR4-dependent chemotaxis. STAM (Signal Transducing Adaptor Molecule) subfamily members have at their N-termini a VHS domain, which is involved in cytokine-mediated intracellular signal transduction and has a superhelical structure similar to the structure of ARM (Armadillo) repeats, followed by a Ubiquitin-Interacting Motif (UIM) and a SH3 (Src Homology 3) domain, which is a well-established protein-protein interaction domain, and a GAT (GGA and TOM) domain. At the C-termini of most vertebrate STAMS, an Immunoreceptor Tyrosine-based Activation Motif (ITAM) is present, which mediates the binding of HRS (hepatocyte growth factor-regulated tyrosine kinase substrate) in endocytic and exocytic machineries. STAM is a component of the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery and together with Hrs, functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles.


Pssm-ID: 340797  Cd Length: 131  Bit Score: 61.62  E-value: 2.31e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  29 IQNACDPTNFEPNLALNLEITDLINSKKgNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTKEFLN 108
Cdd:cd17000     5 VEKATSEMNTAEDWGLILDICDKVGQSR-TGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFAS 83

                          90       100
                  ....*....|....*....|....*....
gi 1808313671 109 ELVRRFPERPPIRATRAQTKILELIEEWR 137
Cdd:cd17000    84 EVSNVLNKGHPKVCEKLKALMVEWTEEFK 112
VHS_GGA3 cd17008
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA3; ADP-ribosylation ...
 25-148 2.37e-11

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA3; ADP-ribosylation factor-binding protein GGA3 (Golgi-localized, Gamma-ear-containing, Arf-binding 3) regulates the trafficking and is required for the lysosomal degradation of BACE (beta-site APP-cleaving enzyme), the protease that initiates the production of beta-amyloid, which causes Alzheimer's disease. It also plays a key role in GABA (+) transmission, which is important in the regulation of anxiety-like behaviors. GGA3 is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340805 [Multi-domain]  Cd Length: 141  Bit Score: 61.97  E-value: 2.37e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINsKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd17008     4 LESWLNKATNPSNRQEDWEYIIGFCDQIN-KELEGPQIAVRLLAHKIQSPQEWEALQALTVLEACMKNCGRRFHNEVGKF 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1808313671 105 EFLNELVRRF-PERPPIRAT-RAQTKILELIEEWRQTICQTSKYKE 148
Cdd:cd17008    83 RFLNELIKVVsPKYLGDRVSeKVKTKVIELLYSWTVALPEEAKIKD 128
GAT_GGA_meta cd14234
canonical GAT domain found in metazoan ADP-ribosylation factor (Arf)-binding proteins (GGAs); ...
 242-320 3.61e-11

canonical GAT domain found in metazoan ADP-ribosylation factor (Arf)-binding proteins (GGAs); GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. Moreover, GGAs play important roles in ubiquitin-dependent sorting of cargo proteins both in biosynthetic and endocytic pathways. Three GGAs (GGA1, GGA2, and GGA3) have been identified in mammals. They may appear to behave similarly, since all of them have a multidomain structure consisting of: an N-terminal VHS (Vps27/Hrs/Stam) domain that binds the acidic-cluster dileucine (DxxLL)-type sorting signals (where x is any amino acid) found in the cytoplasmic tail of TGN sorting receptors; a GAT (GGA and TOM) domain that interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and the tumor susceptibility gene 101 product (TSG101); a largely unstructured hinge region that contains clathrin-binding motifs; and a C-terminal GAE (gamma-adaptin ear homology) domain that binds accessory proteins. However, the three GGAs have some differences, which suggest they may possess their own distinct roles. For instance, both GGA1 and GGA3, but not GGA2, contains an internal DxxLL motif that binds to it own VHS domain. Only a portion of the VHS domain of GGA2 possesses distant structural homology to that of GGA1 or GGA3. Moreover, the binding affinity of GGA2 to ubiquitin is quite lower than that of GGA1 or GGA3. In addition, GGA3 has a short splicing variant that is predominantly expressed in human cell lines and tissues except the brain. It does have a VHS domain, but it is unable to bind to the DxxLL motif. GGA2 and GGA3 undergo epidermal growth factor (EGF)-induced phosphorylation.


Pssm-ID: 410582  Cd Length: 84  Bit Score: 59.55  E-value: 3.61e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 242 EVLKIQEKARLLEDMLQGHNAGE-EIKEGDVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRTIERYKLV 320
Cdd:cd14234     5 ELEEVNNNVKLLNEMLDHYSPGEsSQDELELMKELYESCEKMRPTLFRLASETDDDDEGLADILQANDELTRVMQRYKNL 84
VHS_GGA2 cd17010
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation ...
 25-164 6.61e-10

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA2 (Golgi-localized, Gamma-ear-containing, Arf-binding 2) is also called Gamma-adaptin-related protein 2 and VHS domain and ear domain of gamma-adaptin (Vear). It is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340807  Cd Length: 139  Bit Score: 57.59  E-value: 6.61e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINsKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd17010     2 LESWLNKATDPSNSEEDWECIQGFCEQVN-KEPEGPQIATRLLAHKIQSPQEREALHALTVLEMCMNNCGERFHSEVAKF 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1808313671 105 EFLNELVRRFpeRPPIRAT----RAQTKILELIEEWrqticqTSKYKEDLGfIRDMHRLLSYKG 164
Cdd:cd17010    81 RFLNELIKVL--SPKYLGTwsseKVKSRVIEVLFSW------TVWLPEEVK-IRDAYQMLKKQG 135
GAT_GGA cd14230
canonical GAT domain found in metazoan and fungal ADP-ribosylation factor (Arf)-binding ...
 245-320 1.22e-09

canonical GAT domain found in metazoan and fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs); GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. GGAs also play important roles in ubiquitin-dependent sorting of cargo proteins both in biosynthetic and endocytic pathways. The family includes three GGAs (GGA1, GGA2, and GGA3) identified in mammals and two GGAs (Gga1p and Gga2p) identified in the budding yeast Saccharomyces cerevisiae. All these GGAs have a multidomain structure consisting of: an N-terminal VHS (Vps27/Hrs/Stam) domain that binds acidic-cluster dileucine (DxxLL)-type sorting signals (where x is any amino acid) found in the cytoplasmic tail of TGN sorting receptors; a GAT (GGA and TOM) domain that interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and the tumor susceptibility gene 101 product (TSG101); a largely unstructured hinge region that contains clathrin-binding motifs; and a C-terminal GAE (gamma-adaptin ear homology) domain that binds accessory proteins. In contrast to other GGAs-like proteins, members of this family contain a GAT N-terminal region, a helix-loop-helix in the complex with Arf1-GTP.


Pssm-ID: 410578  Cd Length: 80  Bit Score: 55.25  E-value: 1.22e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1808313671 245 KIQEKARLLEDMLQGHNAGEEIKEG-DVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSIHRTIERYKLV 320
Cdd:cd14230     4 EAKNNVDLLDEMLLSYSQEDSSDGDnELMKELYDQLENMRPTLFKLASETEENDNSLGDVLQASDNVNRVINKYKDI 80
VHS_GGA1 cd17009
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation ...
 25-136 8.87e-09

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA1 (Golgi-localized, Gamma-ear-containing, Arf-binding 1) is also called Gamma-adaptin-related protein 1. It is expressed in human brain and affects the generation of amyloid beta-peptide, and may be involved in the pathogenesis of Alzheimer disease. It is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340806 [Multi-domain]  Cd Length: 139  Bit Score: 54.60  E-value: 8.87e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  25 LQRYIQNACDPTNFEPNLALNLEITDLINsKKGNAPREAAVAIVNLINSRNPNVSMLALTLLDICVKNCGYPFHLQISTK 104
Cdd:cd17009     2 LEARINRATNPLNKELDWASINGFCEQLN-EEFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKF 80

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1808313671 105 EFLNELVRRFpeRPPIRATRA----QTKILELIEEW 136
Cdd:cd17009    81 RFLNELIKVV--SPKYLGSRTpekvKNKILELLYSW 114
VHS_Tom1L1 cd16997
VHS (Vps27/Hrs/STAM) domain of TOM1-like protein 1; TOM1-like protein 1 (Tom1L1) is also ...
 48-138 5.20e-07

VHS (Vps27/Hrs/STAM) domain of TOM1-like protein 1; TOM1-like protein 1 (Tom1L1) is also called Src-activating and signaling molecule protein (Srcasm). It is a member of the Tom1 (Target of myb1) subfamily, characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. They are novel regulators for post-Golgi trafficking and signaling. Tom1L1 has been implicated in multivesicular body (MVB) formation, viral egress from the cell, and cytokinesis. Its amplification enhances the metastatic progression of ERBB2-positive breast cancers. The VHS domain has a superhelical structure similar to the structure of the ARM repeats and is present at the very N-termini of proteins. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340794  Cd Length: 137  Bit Score: 49.44  E-value: 5.20e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  48 ITDLINSKKgNAPREAAVAIVNLInSRNPNVS--MLALTLLDICVKNCGYPFHLQISTKEFLNE-LVRRFPER--PPIra 122
Cdd:cd16997    25 ICDIINTTE-DGPKDAVKALKKRI-SKNYNHKeiRLTLSLLDMCMQNCGPSFQSLVVKKEFVKDvLVKLLNPRynLPV-- 100

                          90
                  ....*....|....*.
gi 1808313671 123 tRAQTKILELIEEWRQ 138
Cdd:cd16997   101 -DLQNKILNFIMTWAQ 115
GAT_GGA_Tom1-like cd21383
canonical GAT domain found in eukaryotic ADP-ribosylation factor (Arf)-binding proteins (GGAs), ...
 246-317 4.63e-06

canonical GAT domain found in eukaryotic ADP-ribosylation factor (Arf)-binding proteins (GGAs), metazoan myb protein 1 (Tom1)-like proteins, and similar proteins; This model represents the canonical GAT (GGA and Tom1) domain found in GGAs from eukaryotes, Tom1-like proteins from metazoa, and LAS seventeen-binding protein 5 (Lsb5p)-like proteins from fungi. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin. GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. GGAs play important roles in ubiquitin-dependent sorting of cargo proteins both in biosynthetic and endocytic pathways. Tom1 and its related proteins, Tom1L1 and Tom1L2, form a protein family sharing an N-terminal VHS-domain followed by a GAT domain. Tom1 family proteins bind to ubiquitin, ubiquitinated proteins, and Toll-interacting protein (Tollip) through its GAT domain. They do not associate with either Arf GTPases through its GAT domain nor with acidic cluster-dileucine sequences through its VHS domain. In addition, Tom1 family proteins recruit clathrin onto endosomes through their C-terminal region. The C-terminal clathrin-binding region of Tom1 and Tom1L2 are similar to each other, but distinguishable from Tom1L1.


Pssm-ID: 410588  Cd Length: 80  Bit Score: 44.95  E-value: 4.63e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1808313671 246 IQEKARLLEDMLqGHNAGEEIKEGDVYEELVNALASAHPKIQKMCEEESD--DTEAVAKLFEINDSIHRTIERY 317
Cdd:cd21383     5 VKENASLLNEML-SAASPEELLDNELLQELVEFCRACQPRIVELIEATTDglDEDTLEELLELNDELNEALQRY 77
GAT_GGA-like_plant cd14231
canonical GAT domain found in uncharacterized Golgi-localized gamma ear-containing Arf-binding ...
 252-318 5.72e-04

canonical GAT domain found in uncharacterized Golgi-localized gamma ear-containing Arf-binding protein (GGA)-like proteins mainly found in plants; The family includes a group of uncharacterized plant proteins containing an N-terminal VHS (Vps27p/Hrs/STAM)-domain and a GAT (GGA and TOM1) domain. Both domains are also present in Golgi-localized gamma ear-containing Arf-binding proteins (GGAs), which belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. In contrast to GGA proteins, members in this family do not have either a GAE (gamma-adaptin ear homology) domain or a clathrin-binding motif. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin.


Pssm-ID: 410579  Cd Length: 79  Bit Score: 39.17  E-value: 5.72e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1808313671 252 LLEDMLQGHNAG--EEIKEgDVYEELVNALASAHPKIQKMCEEESDDtEAVAKLFEINDSIHRTIERYK 318
Cdd:cd14231    11 LLSDMLNAVDPRdpEALKD-ELTVDLVEQCRQSQPRVMQLVESTGDE-GLLFQALELNDELQRVLSKHD 77
GGA_N-GAT pfam18308
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 198-230 9.23e-04

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


Pssm-ID: 465702  Cd Length: 39  Bit Score: 37.28  E-value: 9.23e-04
                          10        20        30
                  ....*....|....*....|....*....|...
gi 1808313671 198 QSAKLQELIRRGSPHDLQEANKLMKVMAGYDNR 230
Cdd:pfam18308   6 KSKLLARLLKSKNPEDLQAANRLIKSMVKEDDE 38
GAT_GGA1_GGA2 cd14239
canonical GAT domain found in ADP-ribosylation factor (Arf)-binding proteins GGA1 and GGA2; ...
 232-318 1.43e-03

canonical GAT domain found in ADP-ribosylation factor (Arf)-binding proteins GGA1 and GGA2; This subfamily includes GGA1 and GGA2, both of which belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. GGA1, also called gamma-adaptin-related protein 1, or Golgi-localized gamma ear-containing Arf-binding protein 1, regulates the low-density lipoprotein and sorting receptor LR11/SorLA endocytic traffic and further alters amyloid-beta precursor protein (APP) intracellular distribution and amyloid-beta production. It is also critical for the effects of beta-site APP-cleaving enzyme-1 (BACE1) on amyloid-beta generation. It interacts with BACE1 and promotes its traffic from early endosomes to late endocytic compartments or the TGN. Moreover, GGA1 acts as a clathrin assembly protein with the ability to polymerize clathrin into tubules. GGA2, also called gamma-adaptin-related protein 2, Golgi-localized gamma ear-containing Arf-binding protein 2, or VHS domain and ear domain of gamma-adaptin (Vear), interacts with the acidic cluster-dileucine motif in the cytoplasmic tail of the cation-independent mannose 6-phosphate receptor (CI-MPR) and further plays a major role in the sorting of lysosomal enzymes. It also mediates a vital function that cannot be compensated for by GGA1 and/or GGA3. Both GGA1 and GGA2 have a multidomain structure consisting of an N-terminal VHS (Vps27/Hrs/Stam) domain, a GAT (GGA and TOM) domain, a largely unstructured hinge region that contains clathrin-binding motifs, and a C-terminal GAE (gamma-adaptin ear homology) domain. The GAT domain of GGAs interacts with class I GTP-bound form of Arf proteins, Rabaptin-5, ubiquitin, and/or the tumor susceptibility gene 101 product (TSG101).


Pssm-ID: 410586  Cd Length: 88  Bit Score: 38.11  E-value: 1.43e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 232 KTDYRAKAAEEVlkiQEKARLLEDMLQGHNAGEEIKEG-DVYEELVNALASAHPKIQKMCEEESDDTEAVAKLFEINDSI 310
Cdd:cd14239     1 KVSKRVNAIEEV---NNNVKLLTEMLMSYSRGELSESSeELMKELYQRCEKMRPTLFRLASDTEDNDEALAEILQANDNL 77


                  ....*...
gi 1808313671 311 HRTIERYK 318
Cdd:cd14239    78 TQVINLYK 85
PRK14971 PRK14971
DNA polymerase III subunit gamma/tau;
410-505 3.22e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 237874 [Multi-domain]  Cd Length: 614  Bit Score: 40.53  E-value: 3.22e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671 410 SDGLGNSGLPQPAQSTQPTIS-SMNQSNLSQQVAPS-QPSTQTKPNYDPFGPISNAQTTSKPATPTPSLGS---PPPQHQ 484
Cdd:PRK14971  364 QKGDDASGGRGPKQHIKPVFTqPAAAPQPSAAAAASpSPSQSSAAAQPSAPQSATQPAGTPPTVSVDPPAAvpvNPPSTA 443

                          90       100
                  ....*....|....*....|.
gi 1808313671 485 AQQTRIDPFASLNAPAPRQAS 505
Cdd:PRK14971  444 PQAVRPAQFKEEKKIPVSKVS 464
PHA03247 PHA03247
large tegument protein UL36; Provisional
 402-562 6.33e-03

large tegument protein UL36; Provisional


Pssm-ID: 223021 [Multi-domain]  Cd Length: 3151  Bit Score: 39.92  E-value: 6.33e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  402 GGGISLG---SSDGLGNSGLPQPAQSTQPTISSMNQSNLSQQVA-----------PSQPSTQTKPNYDPFGPisnAQTTS 467
Cdd:PHA03247  2853 GGSVAPGgdvRRRPPSRSPAAKPAAPARPPVRRLARPAVSRSTEsfalppdqperPPQPQAPPPPQPQPQPP---PPPQP 2929

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1808313671  468 KPATPTPSLGSPPPQHQAqQTRIDPFASLNAPAPRQASPFQFQqsiqqpssqpsslLDLTTSNASQTLPVVSTPDQQSN- 546
Cdd:PHA03247  2930 QPPPPPPPRPQPPLAPTT-DPAGAGEPSGAVPQPWLGALVPGR-------------VAVPRFRVPQPAPSREAPASSTPp 2995

                          170
                   ....*....|....*...
gi 1808313671  547 --GAATSADDEWTFSSAL 562
Cdd:PHA03247  2996 ltGHSLSRVSSWASSLAL 3013
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH