|
Name |
Accession |
Description |
Interval |
E-value |
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
9-511 |
0e+00 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 725.31 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 9 ETFNAADyFVDRNIREGRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA 88
Cdd:cd05959 1 EKYNAAT-LVDLNLNEGRGDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 89 VPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLR-FLKHIIVVNGQAP-AGTISFDSFVAGASDQLEAAP 166
Cdd:cd05959 80 VPVPVNTLLTPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEhTLVVLIVSGGAGPeAGALLLAELVAAEAEQLKPAA 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 167 ITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPV 246
Cdd:cd05959 160 THADDPAFWLYSSGSTGRPKGVVHLHADIYWTAELYARNVLGIREDDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMPE 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 247 RPVPASVYETIARYRPTLYFGVPTLYGSMLQEEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSH 322
Cdd:cd05959 240 RPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPNlpsrDLSSLRLCVSAGEALPAEVGERWKARFGLDILDGIGSTEMLH 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 323 IFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEE 402
Cdd:cd05959 320 IFLSNRPGRVRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTFQGEWTRTGDKYVRDDD 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 403 GYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTL 482
Cdd:cd05959 400 GFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRL 479
|
490 500
....*....|....*....|....*....
gi 700405679 483 AHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05959 480 APYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
9-511 |
0e+00 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 652.29 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 9 ETFNAADYFVDRNIREGRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA 88
Cdd:TIGR02262 1 EKYNAAEDLLDRNVVEGRGGKTAFIDDISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 89 VPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVnGQAPAGTISFDSFVAGASDQLEAAPIT 168
Cdd:TIGR02262 81 VPVALNTLLTADDYAYMLEDSRARVVFVSGALLPVIKAALGKSPHLEHRVVV-GRPEAGEVQLAELLATESEQFKPAATQ 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRP 248
Cdd:TIGR02262 160 ADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLMGERP 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPASVYETIARYRPTLYFGVPTLYGSMLQEEGSL----DGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIF 324
Cdd:TIGR02262 240 TPDAVFDRLRRHQPTIFYGVPTLYAAMLADPNLPsedqVRLRLCTSAGEALPAEVGQRWQARFGVDIVDGIGSTEMLHIF 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGY 404
Cdd:TIGR02262 320 LSNLPGDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLISGPSSATMYWNNRAKSRDTFQGEWTRSGDKYVRNDDGS 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYapdDQLKKDLKEYVKKTLAH 484
Cdd:TIGR02262 400 YTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIKPKAFVVLRPGQ---TALETELKEHVKDRLAP 476
|
490 500
....*....|....*....|....*..
gi 700405679 485 YKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:TIGR02262 477 YKYPRWIVFVDDLPKTATGKIQRFKLR 503
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
8-512 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 583.23 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 8 PETFNAADYFVDRNIrEGRGQKTAILCRDNA-----FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFG 82
Cdd:COG0365 5 GGRLNIAYNCLDRHA-EGRGDKVALIWEGEDgeertLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 83 AIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELY---------SKFEPIKDKLRFLKHIIVVNGQAP----AGTI 149
Cdd:COG0365 84 CARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLrggkvidlkEKVDEALEELPSLEHVIVVGRTGAdvpmEGDL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 150 SFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGN 229
Cdd:COG0365 164 DWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGHSY 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 230 GLYFPFSVGATSVLM---PVRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEG------SLDGVRLCVSAGEALPADIF 300
Cdd:COG0365 244 IVYGPLLNGATVVLYegrPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDeplkkyDLSSLRLLGSAGEPLNPEVW 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 301 KRWQQRHGVEIIDGIGSTELSHIFISNLPG-KAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGD--SAAAAYWN 377
Cdd:COG0365 324 EWWYEAVGVPIVDGWGQTETGGIFISNLPGlPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPwpGMFRGYWN 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 378 KHQKTKSTMLG---DWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKP 454
Cdd:COG0365 404 DPERYRETYFGrfpGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVV 483
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 455 KAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:COG0365 484 KAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRK 541
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
29-511 |
0e+00 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 536.27 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpDDACFWLYSSGSTGQPKGT 188
Cdd:cd05919 81 CEARLVVTSA---------------------------------------------------DDIAYLLYSSGTTGPPKGV 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 189 IHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASVYETIARYRPTLYFGV 268
Cdd:cd05919 110 MHAHRDPLLFADAMAREALGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNPGWPTAERVLATLARFRPTVLYGV 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 269 PTLYGSMLQEEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGY 344
Cdd:cd05919 190 PTFYANLLDSCAgspdALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGHIFLSNRPGAWRLGSTGRPVPGY 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 345 DARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSP 424
Cdd:cd05919 270 EIRLVDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATFNGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSP 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 425 VEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:cd05919 350 VEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGK 429
|
....*..
gi 700405679 505 LKRFELR 511
Cdd:cd05919 430 LQRFKLR 436
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
29-511 |
6.48e-170 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 486.99 E-value: 6.48e-170
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:cd05958 1 RTCLRSPEREWTYRDLLALANRIANVLVGeLGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapITPDDACFWLYSSGSTGQPKG 187
Cdd:cd05958 81 KARITVALCAHAL----------------------------------------------TASDDICILAFTSGTTGAPKA 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLYFG 267
Cdd:cd05958 115 TMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLE-EATPDLLLSAIARYKPTVLFT 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 268 VPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPG 343
Cdd:cd05958 194 APTAYRAMLahpdAAGPDLSSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMFHIFISARPGDARPGATGKPVPG 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 344 YDARIVDENFQDIPVGEVGTLLIKGDSAaaaYWNKHQKTKSTML-GDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWV 422
Cdd:cd05958 274 YEAKVVDDEGNPVPDGTIGRLAVRGPTG---CRYLADKRQRTYVqGGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNI 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 423 SPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTAT 502
Cdd:cd05958 351 APPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHAKAHIAPYKYPRAIEFVTELPRTAT 430
|
....*....
gi 700405679 503 GKLKRFELR 511
Cdd:cd05958 431 GKLQRFALR 439
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
17-512 |
3.65e-138 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 406.50 E-value: 3.65e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 17 FVDRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTL 96
Cdd:COG0318 4 LLRRAAAR-HPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 97 MQPKDYLYFLNDSRAKILIideelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpddACFWL 176
Cdd:COG0318 83 LTAEELAYILEDSGARALV--------------------------------------------------------TALIL 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYET 256
Cdd:COG0318 107 YTSGTTGRPKGVMLTHRNLLANAAAIA-AALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLP-RFDPERVLEL 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVPTLYGSMLQEEGS----LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNL--PG 330
Cdd:COG0318 185 IERERVTVLFGVPTMLARLLRHPEFarydLSSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVTVNPedPG 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 KAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGR 410
Cdd:COG0318 265 ERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAFRDGWLRTGDLGRLDEDGYLYIVGR 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 411 SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRW 490
Cdd:COG0318 345 KKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDAE---ELRAFLRERLARYKVPRR 421
|
490 500
....*....|....*....|..
gi 700405679 491 IEFVDDLPKTATGKLKRFELRA 512
Cdd:COG0318 422 VEFVDELPRTASGKIDRRALRE 443
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
26-512 |
5.39e-125 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 375.29 E-value: 5.39e-125
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYF 105
Cdd:PRK06187 19 HPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRLKPEEIAYI 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNGQAPAGTI----SFDSFVAGASDQLEAAPITPDDACFWLYSSGS 181
Cdd:PRK06187 99 LNDAEDRVVLVDSEFVPLLAAILPQLPTVRTVIVEGDGPAAPLApevgEYEELLAAASDTFDFPDIDENDAAAMLYTSGT 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 182 TGQPKGTIH-----LQHDMVyaaetyGKQVLGIQEHDICFSAAKLFFAYGLGNGlYFPFSVGATSVlMPVRPVPASVYET 256
Cdd:PRK06187 179 TGHPKGVVLshrnlFLHSLA------VCAWLKLSRDDVYLVIVPMFHVHAWGLP-YLALMAGAKQV-IPRRFDPENLLDL 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVPTLYGSMLQEEGS----LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLP--- 329
Cdd:PRK06187 251 IETERVTFFFAVPTIWQMLLKAPRAyfvdFSSLRLVIYGGAALPPALLREFKEKFGIDLVQGYGMTETSPVVSVLPPedq 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 330 ---GKAKPGSSGKVVPGYDARIVDENFQDIPV--GEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGY 404
Cdd:PRK06187 331 lpgQWTKRRSAGRPLPGVEARIVDDDGDELPPdgGEVGEIIVRGPWLMQGYWNRPEATAETIDGGWLHTGDVGYIDEDGY 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDqlkKDLKEYVKKTLAH 484
Cdd:PRK06187 411 LYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDA---KELRAFLRGRLAK 487
|
490 500
....*....|....*....|....*...
gi 700405679 485 YKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK06187 488 FKLPKRIAFVDELPRTSVGKILKRVLRE 515
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
19-511 |
3.16e-122 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 366.12 E-value: 3.16e-122
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 19 DRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQ 98
Cdd:cd05936 6 EEAARR-FPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 99 PKDYLYFLNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtISFDSFVAGASDQLEAAPITPDDACFWLYS 178
Cdd:cd05936 85 PRELEHILNDSGAKALIVA-------------------------------VSFTDLLAAGAPLGERVALTPEDVAVLQYT 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 179 SGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEH-DICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETI 257
Cdd:cd05936 134 SGTTGVPKGAMLTHRNLVANALQIKAWLEDLLEGdDVVLAALPLFHVFGLTVALLLPLALGATIVLIP-RFRPIGVLKEI 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISN-LPGKA 332
Cdd:cd05936 213 RKHRVTIFPGVPTMYIALLNapefKKRDFSSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSPVVAVNpLDGPR 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 KPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSN 412
Cdd:cd05936 293 KPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFVDGWLRTGDIGYMDEDGYFFIVDRKK 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 413 DMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIE 492
Cdd:cd05936 373 DMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLT---EEEIIAFCREQLAGYKVPRQVE 449
|
490
....*....|....*....
gi 700405679 493 FVDDLPKTATGKLKRFELR 511
Cdd:cd05936 450 FRDELPKSAVGKILRRELR 468
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
39-512 |
1.15e-112 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 340.47 E-value: 1.15e-112
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ElyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpdDACFWLYSSGSTGQPKGTIHlQHDMVYA 198
Cdd:cd05972 81 E---------------------------------------------------DPALIYFTSGTTGLPKGVLH-THSYPLG 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 199 AETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRP-VPASVYETIARYRPTLYFGVPTLYGSMLQ 277
Cdd:cd05972 109 HIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRfDAERILELLERYGVTSFCGPPTAYRMLIK 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 278 EEGS---LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELShIFISNLPG-KAKPGSSGKVVPGYDARIVDENF 353
Cdd:cd05972 189 QDLSsykFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETG-LTVGNFPDmPVKPGSMGRPTPGYDVAIIDDDG 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 354 QDIPVGEVGTLLIKGD--SAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACL 431
Cdd:cd05972 268 RELPPGEEGDIAIKLPppGLFLGYVGDPEKTEASIRGDYYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESAL 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 432 IEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05972 348 LEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELR 427
|
.
gi 700405679 512 A 512
Cdd:cd05972 428 D 428
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
171-506 |
3.48e-110 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 330.79 E-value: 3.48e-110
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 171 DACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAyGLGNGLYFPFSVGATSVLMPvRPVP 250
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALA-ASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLP-KFDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 251 ASVYETIARYRPTLYFGVPTLYGSMLQEEGS----LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFIS 326
Cdd:cd04433 78 EAALELIEREKVTILLGVPTLLARLLKAPESagydLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTETGGTVAT 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NLP--GKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGY 404
Cdd:cd04433 158 GPPddDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVDEDGWYRTGDLGRLDEDGY 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGyapDDQLKKDLKEYVKKTLAH 484
Cdd:cd04433 238 LYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPG---ADLDAEELRAHVRERLAP 314
|
330 340
....*....|....*....|..
gi 700405679 485 YKYPRWIEFVDDLPKTATGKLK 506
Cdd:cd04433 315 YKVPRRVVFVDALPRTASGKID 336
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
12-512 |
1.59e-101 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 316.45 E-value: 1.59e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 12 NAADYFVDRNIREGRGQKTAILC----RDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLSFFGAIKI 86
Cdd:PRK04319 43 NIAYEAIDRHADGGRKDKVALRYldasRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIpELY-FALLGALKN 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 87 GAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFepIKDKLRFLKHIIVVN--GQAPAGTISFDSFVAGASDQLEA 164
Cdd:PRK04319 122 GAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLERK--PADDLPSLKHVLLVGedVEEGPGTLDFNALMEQASDEFDI 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 165 APITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETyGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLM 244
Cdd:PRK04319 200 EWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQT-GKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVID 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 245 PVRPVPASVYETIARYRPTLYFGVPTLYgSMLQEEGS-------LDGVRLCVSAGEAL-PADIfkRWQQR-HGVEIIDGI 315
Cdd:PRK04319 279 GGRFSPERWYRILEDYKVTVWYTAPTAI-RMLMGAGDdlvkkydLSSLRHILSVGEPLnPEVV--RWGMKvFGLPIHDNW 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 316 GSTELSHIFISNLPGKA-KPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGD--SAAAAYWNKHQKTKSTMLGDWVN 392
Cdd:PRK04319 356 WMTETGGIMIANYPAMDiKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIKKGwpSMMRGIWNNPEKYESYFAGDWYV 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 393 TDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD--KNLVkpKAFVVLKRGYAPDDQL 470
Cdd:PRK04319 436 SGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPvrGEII--KAFVALRPGYEPSEEL 513
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 700405679 471 KKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK04319 514 KEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKA 555
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
27-512 |
1.29e-100 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 312.22 E-value: 1.29e-100
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFL 106
Cdd:PRK07656 19 GDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTADEAAYIL 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 107 NDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVV----NGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGST 182
Cdd:PRK07656 99 ARGDAKALFVLGLFLGVDYSATTRLPALEHVVICeteeDDPHTEKMKTFTDFLAAGDPAERAPEVDPDDVADILFTSGTT 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 183 GQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRpVPASVYETIARYRP 262
Cdd:PRK07656 179 GRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVNAPLMRGATILPLPVF-DPDEVFRLIETERI 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQEEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEII-DGIGSTELSHIFISNLPG---KAKP 334
Cdd:PRK07656 257 TVLPGPPTMYNSLLQHPDrsaeDLSSLRLAVTGAASMPVALLERFESELGVDIVlTGYGLSEASGVTTFNRLDddrKTVA 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 335 GSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSND 413
Cdd:PRK07656 337 GTIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATAAAIDADgWLHTGDLGRLDEEGYLYIVDRKKD 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 414 MLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEF 493
Cdd:PRK07656 417 MFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEF 493
|
490
....*....|....*....
gi 700405679 494 VDDLPKTATGKLKRFELRA 512
Cdd:PRK07656 494 LDELPKNATGKVLKRALRE 512
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
18-505 |
4.04e-100 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 308.39 E-value: 4.04e-100
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 18 VDRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLM 97
Cdd:cd17631 1 LRRRARR-HPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 98 QPKDYLYFLNDSRAKILIideelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpDDACFWLY 177
Cdd:cd17631 80 TPPEVAYILADSGAKVLF------------------------------------------------------DDLALLMY 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIHLQHDMVYAAETYGKQvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETI 257
Cdd:cd17631 106 TSGTTGRPKGAMLTHRNLLWNAVNALAA-LDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILR-KFDPETVLDLI 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFGVPTLYGSMLQEEGS----LDGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIfISNLPGK-- 331
Cdd:cd17631 184 ERHRVTSFFLVPTMIQALLQHPRFattdLSSLRAVIYGGAPMPERLLRALQAR-GVKFVQGYGMTETSPG-VTFLSPEdh 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 332 -AKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGR 410
Cdd:cd17631 262 rRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWFHTGDLGRLDEDGYLYIVDR 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 411 SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRW 490
Cdd:cd17631 342 KKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDED---ELIAHCRERLARYKIPKS 418
|
490
....*....|....*
gi 700405679 491 IEFVDDLPKTATGKL 505
Cdd:cd17631 419 VEFVDALPRNATGKI 433
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
39-512 |
2.00e-97 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 301.73 E-value: 2.00e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSpELY-FSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapITPDDACFWLYSSGSTGQPKGTIHLQHDMVY 197
Cdd:cd05969 80 EELYER-------------------------------------------TDPEDPTLLHYTSGTTGTPKGVLHVHDAMIF 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 198 AAETyGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTL------ 271
Cdd:cd05969 117 YYFT-GKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRFDAESWYGIIERVKVTVWYTAPTAirmlmk 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 YGSMLQEEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPG-KAKPGSSGKVVPGYDARIVD 350
Cdd:cd05969 196 EGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFGVPIHDTWWQTETGSIMIANYPCmPIKPGSMGKPLPGVKAAVVD 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 351 ENFQDIPVGEVGTLLIKGD--SAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVE 428
Cdd:cd05969 276 ENGNELPPGTKGILALKPGwpSMFRGIWNDEERYKNSFIDGWYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVE 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 429 ACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRF 508
Cdd:cd05969 356 SALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRR 435
|
....
gi 700405679 509 ELRA 512
Cdd:cd05969 436 VLKA 439
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
5-511 |
3.83e-93 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 293.63 E-value: 3.83e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 5 LNLPETFNAADYFVDRNIREGRGQKTAILCRDNA----FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLS 79
Cdd:cd05970 10 INVPENFNFAYDVVDAMAKEYPDKLALVWCDDAGeeriFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRyEFW-YS 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 80 FFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE--LYSKFEPIKDKLRFLKHIIVVNGQAPAGTISFDSFVAG 157
Cdd:cd05970 89 LLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAEdnIPEEIEKAAPECPSKPKLVWVGDPVPEGWIDFRKLIKN 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 158 ASDQLE--AAPITP--DDACFWLYSSGSTGQPKgtiHLQHDMVYAAE--TYGKQVLGIQEHDICFSAAKLFFAYGLGNGL 231
Cdd:cd05970 169 ASPDFErpTANSYPcgEDILLVYFSSGTTGMPK---MVEHDFTYPLGhiVTAKYWQNVREGGLHLTVADTGWGKAVWGKI 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 232 YFPFSVG-ATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEGS---LDGVRLCVSAGEALPADIFKRWQQRH 307
Cdd:cd05970 246 YGQWIAGaAVFVYDYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSrydLSSLRYCTTAGEALNPEVFNTFKEKT 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 308 GVEIIDGIGSTELShIFISNLPG-KAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAA-----AAYWNKHQK 381
Cdd:cd05970 326 GIKLMEGFGQTETT-LTIATFPWmEPKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSKGKpvglfGGYYKDAEK 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 382 TKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLK 461
Cdd:cd05970 405 TAEVWHDGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLA 484
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 700405679 462 RGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05970 485 KGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
24-418 |
1.28e-91 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 285.75 E-value: 1.28e-91
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 24 EGRGQKTAILC-RDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDY 102
Cdd:pfam00501 6 ARTPDKTALEVgEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLPAEEL 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 103 LYFLNDSRAKILIIDEELY-SKFEPIKDKLRFLKHIIVV-NGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSG 180
Cdd:pfam00501 86 AYILEDSGAKVLITDDALKlEELLEALGKLEVVKLVLVLdRDPVLKEEPLPEEAKPADVPPPPPPPPDPDDLAYIIYTSG 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 181 STGQPKGTIHLQHDMVYAAETY---GKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMP--VRPVPASVYE 255
Cdd:pfam00501 166 TTGKPKGVMLTHRNLVANVLSIkrvRPRGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATVVLPPgfPALDPAALLE 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPG- 330
Cdd:pfam00501 246 LIERYKVTVLYGVPTLLNMLLEagapKRALLSSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTGVVTTPLPLd 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 --KAKPGSSGKVVPGYDARIVDENF-QDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML-GDWVNTDDKYAVDEEGYFY 406
Cdd:pfam00501 326 edLRSLGSVGRPLPGTEVKIVDDETgEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDeDGWYRTGDLGRRDEDGYLE 405
|
410
....*....|..
gi 700405679 407 YIGRSNDMLKVG 418
Cdd:pfam00501 406 IVGRKKDQIKLG 417
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
36-511 |
9.80e-89 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 278.41 E-value: 9.80e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpddaCFWLYSSGSTGQPKGTIhLQHDM 195
Cdd:cd05934 81 VDP------------------------------------------------------ASILYTSGTTGPPKGVV-ITHAN 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 196 VYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTL--YFGVPtlyG 273
Cdd:cd05934 106 LTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLP-RFSASRFWSDVRRYGATVtnYLGAM---L 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 274 SML--QEEGSLDG---VRLCVSAgeALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARI 348
Cdd:cd05934 182 SYLlaQPPSPDDRahrLRAAYGA--PNPPELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRI 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 349 VDENFQDIPVGEVGTLLIK---GDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPV 425
Cdd:cd05934 260 VDDDGQELPAGEPGELVIRglrGWGFFKGYYNMPEATAEAMRNGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSA 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 426 EVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:cd05934 340 EVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE---ELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKV 416
|
....*.
gi 700405679 506 KRFELR 511
Cdd:cd05934 417 AKAQLR 422
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
29-511 |
1.92e-88 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 281.05 E-value: 1.92e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PRK08316 27 KTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEELAYILDH 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEELYSKFEPIKDKL---RFLKHIIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQP 185
Cdd:PRK08316 107 SGARAFLVDPALAPTAEAALALLpvdTLILSLVLGGREAPGGWLDFADWAEAGSVAEPDVELADDDLAQILYTSGTESLP 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLY 265
Cdd:PRK08316 187 KGAML-THRALIAEYVSCIVAGDMSADDIPLHALPLYHCAQLDVFLGPYLYVGATNVILD-APDPELILRTIEAERITSF 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 266 FGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELSHIFISNLPG--KAKPGSSG 338
Cdd:PRK08316 265 FAPPTVWISLLRhpdfDTRDLSSLRKGYYGASIMPVEVLKELRERLpGLRFYNCYGQTEIAPLATVLGPEehLRRPGSAG 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 339 KVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVG 418
Cdd:PRK08316 345 RPVLNVETRVVDDDGNDVAPGEVGEIVHRSPQLMLGYWDDPEKTAEAFRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTG 424
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 419 GIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLP 498
Cdd:PRK08316 425 GENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVT---EDELIAHCRARLAGFKVPKRVIFVDELP 501
|
490
....*....|....
gi 700405679 499 KTATGK-LKRfELR 511
Cdd:PRK08316 502 RNPSGKiLKR-ELR 514
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
39-512 |
2.07e-83 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 265.45 E-value: 2.07e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:cd05971 7 VTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTDG 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 elyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKGTIH-----LQH 193
Cdd:cd05971 87 --------------------------------------------------SDDPALIIYTSGTTGPPKGALHahrvlLGH 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMVYaaetYGKQVLGIQEHDICFSAAKLFFAYGLGNGLyFPFSVGATSVLM--PVRPVPASVYETIARYRPTLYFGVPTL 271
Cdd:cd05971 117 LPGV----QFPFNLFPRDGDLYWTPADWAWIGGLLDVL-LPSLYFGVPVLAhrMTKFDPKAALDLMSRYGVTTAFLPPTA 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 YGSMLQEEGSLD--GVRL--CVSAGEALPADIFKrWQQRH-GVEIIDGIGSTElSHIFISNLP--GKAKPGSSGKVVPGY 344
Cdd:cd05971 192 LKMMRQQGEQLKhaQVKLraIATGGESLGEELLG-WAREQfGVEVNEFYGQTE-CNLVIGNCSalFPIKPGSMGKPIPGH 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 345 DARIVDENFQDIPVGEVGTLLIKGDSAAA--AYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWV 422
Cdd:cd05971 270 RVAIVDDNGTPLPPGEVGEIAVELPDPVAflGYWNNPSATEKKMAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRI 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 423 SPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTAT 502
Cdd:cd05971 350 GPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTAT 429
|
490
....*....|
gi 700405679 503 GKLKRFELRA 512
Cdd:cd05971 430 GKIRRRELRA 439
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
50-511 |
3.86e-81 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 262.02 E-value: 3.86e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 50 KVGNALKGL-GIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIK 128
Cdd:cd05928 53 KAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELAPEVDSVA 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 129 DKLRFLKHIIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLG 208
Cdd:cd05928 133 SECPSLKTKLLVSEKSRDGWLNFKELLNEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLD 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 209 IQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSV--LMPvRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEGS---LD 283
Cdd:cd05928 213 LTASDIMWNTSDTGWIKSAWSSLFEPWIQGACVFvhHLP-RFDPLVILKTLSSYPITTFCGAPTVYRMLVQQDLSsykFP 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 284 GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGT 363
Cdd:cd05928 292 SLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGD 371
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 364 LLIKGDSAA-----AAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVL 438
Cdd:cd05928 372 IGIRVKPIRpfglfSGYVDNPEKTAATIRGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVV 451
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 700405679 439 ECAVVGATDDKNLVKPKAFVVLKRGYAPDD--QLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05928 452 ESAVVSSPDPIRGEVVKAFVVLAPQFLSHDpeQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELR 526
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
13-511 |
1.27e-80 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 260.26 E-value: 1.27e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 13 AADYFVDRNI--REGRGQktailcrDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVP 90
Cdd:cd12119 5 AARLHGDREIvsRTHEGE-------VHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 91 ICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNG------QAPAGTISFDSFVAGASDQLEA 164
Cdd:cd12119 78 HTINPRLFPEQIAYIINHAEDRVVFVDRDFLPLLEAIAPRLPTVEHVVVMTDdaampePAGVGVLAYEELLAAESPEYDW 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 165 APITPDDACFWLYSSGSTGQPKGTI--HLQ---HDMVYAAetygKQVLGIQEHDICFSAAKLFFAYGLGnglyFPFS--- 236
Cdd:cd12119 158 PDFDENTAAAICYTSGTTGNPKGVVysHRSlvlHAMAALL----TDGLGLSESDVVLPVVPMFHVNAWG----LPYAaam 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 237 VGATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYGSMLQE----EGSLDGVRLCVSAGEALPADIFKRWQQRhGVEII 312
Cdd:cd12119 230 VGAKLVLPGPYLDPASLAELIEREGVTFAAGVPTVWQGLLDHleanGRDLSSLRRVVIGGSAVPRSLIEAFEER-GVRVI 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 313 DGIGSTELSHIFISNLPGKAKPGSS-----------GKVVPGYDARIVDENFQDIPV--GEVGTLLIKGDSAAAAYWNKH 379
Cdd:cd12119 309 HAWGMTETSPLGTVARPPSEHSNLSedeqlalrakqGRPVPGVELRIVDDDGRELPWdgKAVGELQVRGPWVTKSYYKND 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 380 QKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVV 459
Cdd:cd12119 389 EESEALTEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVV 468
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 700405679 460 LKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd12119 469 LKEGATVT---AEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALR 517
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
36-505 |
3.48e-80 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 258.30 E-value: 3.48e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:cd05911 8 GKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIF 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELYSKFEPIKDKLRFLKHIIVVNGQAPAGT--ISFDSFVAGASDQLEAAP--ITPDDACFWLYSSGSTGQPKG---- 187
Cdd:cd05911 88 TDPDGLEKVKEAAKELGPKDKIIVLDDKPDGVLsiEDLLSPTLGEEDEDLPPPlkDGKDDTAAILYSSGTTGLPKGvcls 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 --TIHLQHDMVYAAETYGKQVlgiQEHDICFSAakLFFAYGLGNGLYFPFSvGATSVLMPvRPVPASVYETIARYRPTLY 265
Cdd:cd05911 168 hrNLIANLSQVQTFLYGNDGS---NDVILGFLP--LYHIYGLFTTLASLLN-GATVIIMP-KFDSELFLDLIEKYKITFL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 266 FGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKV 340
Cdd:cd05911 241 YLVPPIAAALAKspllDKYDLSSLRVILSGGAPLSKELQELLAKRfPNATIKQGYGMTETGGILTVNPDGDDKPGSVGRL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 341 VPGYDARIVDENFQDI-PVGEVGTLLIKGDSAAAAYWNKHQKTKST-MLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVG 418
Cdd:cd05911 321 LPNVEAKIVDDDGKDSlGPNEPGEICVRGPQVMKGYYNNPEATKETfDEDGWLHTGDIGYFDEDGYLYIVDRKKELIKYK 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 419 GIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyaPDDQL-KKDLKEYVKKTLAHYKypRW---IEFV 494
Cdd:cd05911 401 GFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRK----PGEKLtEKEVKDYVAKKVASYK--QLrggVVFV 474
|
490
....*....|.
gi 700405679 495 DDLPKTATGKL 505
Cdd:cd05911 475 DEIPKSASGKI 485
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
38-507 |
1.69e-78 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 254.16 E-value: 1.69e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd05926 14 ALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSkfEPIKDKLRFLKHIIVVNGQAPAGTISFD--SFVAGASDQLEAAPIT---PDDACFWLYSSGSTGQPKGtIHLQ 192
Cdd:cd05926 94 KGELG--PASRAASKLGLAILELALDVGVLIRAPSaeSLSNLLADKKNAKSEGvplPDDLALILHTSGTTGRPKG-VPLT 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 193 HDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGAtSVLMPVRPVPASVYETIARYRPTLYFGVPTLY 272
Cdd:cd05926 171 HRNLAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGG-SVVLPPRFSASTFWPDVRDYNATWYTAVPTIH 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 273 GSMLQEEGSLDG-----VRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISN--LPGKAKPGSSGKVVpGYD 345
Cdd:cd05926 250 QILLNRPEPNPEspppkLRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAHQMTSNplPPGPRKPGSVGKPV-GVE 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 346 ARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSP 424
Cdd:cd05926 329 VRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDgWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISP 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 425 VEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:cd05926 409 LEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVT---EEELRAFCRKHLAAFKVPKKVYFVDELPKTATGK 485
|
...
gi 700405679 505 LKR 507
Cdd:cd05926 486 IQR 488
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
12-512 |
4.97e-78 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 258.42 E-value: 4.97e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 12 NAADYFVDRNIREGRGQKTAILCRDnAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPI 91
Cdd:PRK06060 5 NLAGLLAEQASEAGWYDRPAFYAAD-VVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAF 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 92 CLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKdklrflkhiiVVNgqaPAGTISfDSFVAGASDQleaAPITPDD 171
Cdd:PRK06060 84 LANPELHRDDHALAARNTEPALVVTSDALRDRFQPSR----------VAE---AAELMS-EAARVAPGGY---EPMGGDA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 172 ACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVrPVPA 251
Cdd:PRK06060 147 LAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVINSA-PVTP 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 252 SVYETI-ARYRPTLYFGVPTLYGSMLQ--EEGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELSHIFISN 327
Cdd:PRK06060 226 EAAAILsARFGPSVLYGVPNFFARVIDscSPDSFRSLRCVVSAGEALELGLAERLMEFFgGIPILDGIGSTEVGQTFVSN 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTmlGDWVNTDDKYAVDEEGYFYY 407
Cdd:PRK06060 306 RVDEWRLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDSPVAN--EGWLDTRDRVCIDSDGWVTY 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 408 IGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKY 487
Cdd:PRK06060 384 RCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKV 463
|
490 500
....*....|....*....|....*
gi 700405679 488 PRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK06060 464 PHRFAVVDRLPRTPNGKLVRGALRK 488
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
18-512 |
6.06e-78 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 254.92 E-value: 6.06e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 18 VDRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLM 97
Cdd:PRK05605 38 YDNAVAR-FGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVEHNPLY 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 98 QPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRfLKHIIVVN----------------------------GQAPaGTI 149
Cdd:PRK05605 117 TAHELEHPFEDHGARVAIVWDKVAPTVERLRRTTP-LETIVSVNmiaampllqrlalrlpipalrkaraaltGPAP-GTV 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 150 SFDSFVA----GASDQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQHDMVYAAETYGKQ-VLGIQEHDICFSAA-KLFF 223
Cdd:PRK05605 195 PWETLVDaaigGDGSDVSHPRPTPDDVALILYTSGTTGKPKGAQ-LTHRNLFANAAQGKAwVPGLGDGPERVLAAlPMFH 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 224 AYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGSMLQ---EEG-SLDGVRLCVSAGEALPADI 299
Cdd:PRK05605 274 AYGLTLCLTLAVSIGGELVLLP-APDIDLILDAMKKHPPTWLPGVPPLYEKIAEaaeERGvDLSGVRNAFSGAMALPVST 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 300 FKRWQQRHGVEIIDGIGSTELSHIFISNLPGKA-KPGSSGKVVPGYDARIVD-ENF-QDIPVGEVGTLLIKGDSAAAAYW 376
Cdd:PRK05605 353 VELWEKLTGGLLVEGYGLTETSPIIVGNPMSDDrRPGYVGVPFPDTEVRIVDpEDPdETMPDGEEGELLVRGPQVFKGYW 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 377 NKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVG-ATDD--KNLVk 453
Cdd:PRK05605 433 NRPEETAKSFLDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGlPREDgsEEVV- 511
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 700405679 454 pkAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK05605 512 --AAVVLEPGAALDPE---GLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
26-512 |
7.10e-76 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 249.19 E-value: 7.10e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYF 105
Cdd:PRK06178 46 RPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYE 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEELYSKFEPIKDKLRfLKHIIVVN--------------------GQAPAGTISFDSFVAGASDQLEAA 165
Cdd:PRK06178 126 LNDAGAEVLLALDQLAPVVEQVRAETS-LRHVIVTSladvlpaeptlplpdslrapRLAAAGAIDLLPALRACTAPVPLP 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 166 PITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMp 245
Cdd:PRK06178 205 PPALDALAALNYTGGTTGMPKGCEHTQRDMVYTAAAAYAVAVVGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVLL- 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 VRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEG-------SLDGVRlCVSAGEALPADIFKRWQQRHGVEIID---GI 315
Cdd:PRK06178 284 ARWDAVAFMAAVERYRVTRTVMLVDNAVELMDHPRfaeydlsSLRQVR-VVSFVKKLNPDYRQRWRALTGSVLAEaawGM 362
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 316 GSTELSHIFISNLPG-----KAKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD 389
Cdd:PRK06178 363 TETHTCDTFTAGFQDddfdlLSQPVFVGLPVPGTEFKICDfETGELLPLGAEGEIVVRTPSLLKGYWNKPEATAEALRDG 442
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 390 WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATD-DKNLVkPKAFVVLKRGYapdD 468
Cdd:PRK06178 443 WLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDpDKGQV-PVAFVQLKPGA---D 518
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 700405679 469 QLKKDLKEYVKKTLAHYKYPRwIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK06178 519 LTAAALQAWCRENMAVYKVPE-IRIVDALPMTATGKVRKQDLQA 561
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
39-511 |
1.92e-73 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 239.34 E-value: 1.92e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFEpikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpDDACFWLYSSGSTGQPKGTIH-LQHDMVY 197
Cdd:cd05973 81 ANRHKLD--------------------------------------------SDPFVMMFTSGTTGLPKGVPVpLRALAAF 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 198 AAetYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYGSMLQ 277
Cdd:cd05973 117 GA--YLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLLMA 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 278 ---EEGSLDGVRLCV--SAGEALPADIFKRWQQRHGVEIIDGIGSTELShIFISNLPGKAKP---GSSGKVVPGYDARIV 349
Cdd:cd05973 195 agaEVPARPKGRLRRvsSAGEPLTPEVIRWFDAALGVPIHDHYGQTELG-MVLANHHALEHPvhaGSAGRAMPGWRVAVL 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 350 DENFQDIPVGEVGTLLIkgDSAAA------AYWNKHQKTKStmlGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVS 423
Cdd:cd05973 274 DDDGDELGPGEPGRLAI--DIANSplmwfrGYQLPDTPAID---GGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIG 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 424 PVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATG 503
Cdd:cd05973 349 PFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSG 428
|
....*...
gi 700405679 504 KLKRFELR 511
Cdd:cd05973 429 KIQRFLLR 436
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
26-510 |
4.78e-73 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 240.15 E-value: 4.78e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLY 104
Cdd:PRK06839 15 HPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYeLNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIF 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEELYSKFEPIKDKLrFLKHIIVVNGqaPAGtiSFDSFVAGASDQLEAAPITpddACfwlYSSGSTGQ 184
Cdd:PRK06839 95 QLKDSGTTVLFVEKTFQNMALSMQKVS-YVQRVISITS--LKE--IEDRKIDNFVEKNESASFI---IC---YTSGTTGK 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGTIHLQHDMVYAAeTYGKQVLGIQEHDICFSAAKLFFAYGLGngLY-FPFSVGATSVLMPVRPVPASVYETIARYRPT 263
Cdd:PRK06839 164 PKGAVLTQENMFWNA-LNNTFAIDLTMHDRSIVLLPLFHIGGIG--LFaFPTLFAGGVIIVPRKFEPTKALSMIEKHKVT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTELS-HIF-ISNLPGKAKPGSS 337
Cdd:PRK06839 241 VVMGVPTIHQALINcskfETTNLQSVRWFYNGGAPCPEELMREFIDR-GFLFGQGFGMTETSpTVFmLSEEDARRKVGSI 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK06839 320 GKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEETIQDGWLCTGDLARVDEDGFVYIVGRKKEMIIS 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYApddQLKKDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PRK06839 400 GGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSV---LIEKDVIEHCRLFLAKYKIPKEIVFLKEL 476
|
490
....*....|...
gi 700405679 498 PKTATGKLKRFEL 510
Cdd:PRK06839 477 PKNATGKIQKAQL 489
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
10-505 |
1.09e-72 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 241.33 E-value: 1.09e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 10 TFNAADYFVDRNIREgRGQKTAIL------CRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGA 83
Cdd:cd17634 51 TLNLAANALDRHLRE-NGDRTAIIyegddtSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLAC 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 84 IKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSK-----FEPIKDKLRFL-----KHIIVVN------GQAPAG 147
Cdd:cd17634 130 ARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITADGGVRAgrsvpLKKNVDDALNPnvtsvEHVIVLKrtgsdiDWQEGR 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 148 TISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGL 227
Cdd:cd17634 210 DLWWRDLIAKASPEHQPEAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGH 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 228 GNGLYFPFSVGATSVL---MPVRPVPASVYETIARYRPTLYFGVPTLYGSMLQ------EEGSLDGVRLCVSAGEALPAD 298
Cdd:cd17634 290 SYLLYGPLACGATTLLyegVPNWPTPARMWQVVDKHGVNILYTAPTAIRALMAagddaiEGTDRSSLRILGSVGEPINPE 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 299 IFkRWQQRH----GVEIIDGIGSTELSHIFISNLPGKA--KPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIkGDS-- 370
Cdd:cd17634 370 AY-EWYWKKigkeKCPVVDTWWQTETGGFMITPLPGAIelKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVI-TDPwp 447
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 371 -AAAAYWNKHQKTKSTML----GDWVNTDDKYaVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGA 445
Cdd:cd17634 448 gQTRTLFGDHERFEQTYFstfkGMYFSGDGAR-RDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGI 526
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 446 TDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:cd17634 527 PHAIKGQAPYAYVVLNHGVEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
39-512 |
3.57e-72 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 238.89 E-value: 3.57e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLD-SEFYPLsFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:PRK06155 47 WTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNrIEFLDV-FLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVE 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKFEPIKDKLRFLKHIIVVNGqAPAGTI----SFDSFVAGASdQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQH 193
Cdd:PRK06155 126 AALLAALEAADPGDLPLPAVWLLDA-PASVSVpagwSTAPLPPLDA-PAPAAAVQPGDTAAILYTSGTTGPSKGVC-CPH 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLgNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLYFgvptLYG 273
Cdd:PRK06155 203 AQFYWWGRNSAEDLEIGADDVLYTTLPLFHTNAL-NAFFQALLAGATYVLEP-RFSASGFWPAVRRHGATVTY----LLG 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 274 SML-----QEEGSLD-GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTElSHIFISNLPGKAKPGSSGKVVPGYDAR 347
Cdd:PRK06155 277 AMVsillsQPARESDrAHRVRVALGPGVPAALHAAFRERFGVDLLDGYGSTE-TNFVIAVTHGSQRPGSMGRLAPGFEAR 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 348 IVDENFQDIPVGEVGTLLIKGD---SAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSP 424
Cdd:PRK06155 356 VVDEHDQELPDGEPGELLLRADepfAFATGYFGMPEKTVEAWRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISS 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 425 VEVEACLIEHPAVLECAVVG-----ATDDKnlvkpKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPK 499
Cdd:PRK06155 436 FEVEQVLLSHPAVAAAAVFPvpselGEDEV-----MAAVVLRDGTALEPV---ALVRHCEPRLAYFAVPRYVEFVAALPK 507
|
490
....*....|...
gi 700405679 500 TATGKLKRFELRA 512
Cdd:PRK06155 508 TENGKVQKFVLRE 520
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
29-505 |
4.59e-72 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 238.71 E-value: 4.59e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK08314 26 KTAIVFYGRAISYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREEELAHYVT 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEPIKDKLRfLKHIIVVN------------------------GQAPAGTISFDSFVAgASDQLE 163
Cdd:PRK08314 106 DSGARVAIVGSELAPKVAPAVGNLR-LRHVIVAQysdylpaepeiavpawlraepplqALAPGGVVAWKEALA-AGLAPP 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 164 AAPITPDDACFWLYSSGSTGQPKGTIH----LQHDMVYAAETYGkqvlGIQEhDICFSAAKLFFAYGLGNGLYFPFSVGA 239
Cdd:PRK08314 184 PHTAGPDDLAVLPYTSGTTGVPKGCMHthrtVMANAVGSVLWSN----STPE-SVVLAVLPLFHVTGMVHSMNAPIYAGA 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 240 TSVLMPV--RPVPAsvyETIARYRPTLYFGVPT----LYGSMLQEEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIID 313
Cdd:PRK08314 259 TVVLMPRwdREAAA---RLIERYRVTHWTNIPTmvvdFLASPGLAERDLSSLRYIGGGGAAMPEAVAERLKELTGLDYVE 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 314 GIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKT-KSTMLGD-- 389
Cdd:PRK08314 336 GYGLTETMAQTHSNPPDRPKLQCLGIPTFGVDARVIDpETLEELPPGEVGEIVVHGPQVFKGYWNRPEATaEAFIEIDgk 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 390 -WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYaPDD 468
Cdd:PRK08314 416 rFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEA-RGK 494
|
490 500 510
....*....|....*....|....*....|....*..
gi 700405679 469 QLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:PRK08314 495 TTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
28-512 |
8.88e-72 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 234.88 E-value: 8.88e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLG-IEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFL 106
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGkDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 107 NDSRAKILIideelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpdDACFWLYSSGSTGQPK 186
Cdd:cd05941 81 TDSEPSLVL-------------------------------------------------------DPALILYTSGTTGRPK 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 187 GTIHlQHDMVYAaetygkQVLGIQE------HDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARY 260
Cdd:cd05941 106 GVVL-THANLAA------NVRALVDawrwteDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLP-KFDPKEVAISRLMP 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 261 RPTLYFGVPTLYGSMLQ------------EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELShIFISN- 327
Cdd:cd05941 178 SITVFMGVPTIYTRLLQyyeahftdpqfaRAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIG-MALSNp 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPGKAKPGSSGKVVPGYDARIVDEN-FQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYF 405
Cdd:cd05941 257 LDGERRPGTVGMPLPGVQARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDgWFKTGDLGVVDEDGYY 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDML-KVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLkkDLKEYVKKTLAH 484
Cdd:cd05941 337 WILGRSSVDIiKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAALSLE--ELKEWAKQRLAP 414
|
490 500
....*....|....*....|....*...
gi 700405679 485 YKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:cd05941 415 YKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
12-512 |
2.89e-71 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 238.23 E-value: 2.89e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 12 NAADYFVDRNIREgRGQKTAIL------CRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIK 85
Cdd:cd05966 53 NISYNCLDRHLKE-RGDKVAIIwegdepDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACAR 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 86 IGAVPICL------NTLMQPkdylyfLNDSRAKILIIDEELY--SKFEPIK-------DKLRFLKHIIVV---NGQAP-- 145
Cdd:cd05966 132 IGAVHSVVfagfsaESLADR------INDAQCKLVITADGGYrgGKVIPLKeivdealEKCPSVEKVLVVkrtGGEVPmt 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 146 AG-TISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtihLQHD----MVYAAETYgKQVLGIQEHDICFSAAK 220
Cdd:cd05966 206 EGrDLWWHDLMAKQSPECEPEWMDSEDPLFILYTSGSTGKPKG---VVHTtggyLLYAATTF-KYVFDYHPDDIYWCTAD 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 221 LFFAYGLGNGLYFPFSVGATSVL---MPVRPVPASVYETIARYRPTLYFGVPTLYgSMLQEEG-------SLDGVRLCVS 290
Cdd:cd05966 282 IGWITGHSYIVYGPLANGATTVMfegTPTYPDPGRYWDIVEKHKVTIFYTAPTAI-RALMKFGdewvkkhDLSSLRVLGS 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 291 AGEALPADIFkRWQQRH-GVE---IIDGIGSTELSHIFISNLPGKA--KPGSSGKVVPGYDARIVDENFQDIPVGEVGTL 364
Cdd:cd05966 361 VGEPINPEAW-MWYYEViGKErcpIVDTWWQTETGGIMITPLPGATplKPGSATRPFFGIEPAILDEEGNEVEGEVEGYL 439
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 365 LIKGD--SAAAAYWNKHQKTKSTMLGDWVN---TDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLE 439
Cdd:cd05966 440 VIKRPwpGMARTIYGDHERYEDTYFSKFPGyyfTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAE 519
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 440 CAVVGATDDknlVK---PKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:cd05966 520 AAVVGRPHD---IKgeaIYAFVTLKDGEEPSDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRK 592
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
38-510 |
6.50e-71 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 234.44 E-value: 6.50e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd05904 32 ALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTT 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKFEPIKDKlrflkhiIVVNGQAPAGTISFDSFVAGA-SDQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQH-DM 195
Cdd:cd05904 112 AELAEKLASLALP-------VVLLDSAEFDSLSFSDLLFEAdEAEPPVVVIKQDDVAALLYSSGTTGRSKGVM-LTHrNL 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 196 VYAAETYGKQVLGIQEHDICFSAA-KLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGS 274
Cdd:cd05904 184 IAMVAQFVAGEGSNSDSEDVFLCVlPMFHIYGLSSFALGLLRLGATVVVMP-RFDLEELLAAIERYKVTHLPVVPPIVLA 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 275 M----LQEEGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELSHIFISNLP---GKAKPGSSGKVVPGYDA 346
Cdd:cd05904 263 LvkspIVDKYDLSSLRQIMSGAAPLGKELIEAFRAKFpNVDLGQGYGMTESTGVVAMCFApekDRAKYGSVGRLVPNVEA 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 347 RIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSP 424
Cdd:cd05904 343 KIVDpETGESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEgWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAP 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 425 VEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyaPDDQLK-KDLKEYVKKTLAHYKYPRWIEFVDDLPKTATG 503
Cdd:cd05904 423 AELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRK----PGSSLTeDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSG 498
|
....*..
gi 700405679 504 KLKRFEL 510
Cdd:cd05904 499 KILRKEL 505
|
|
| pimA |
TIGR03205 |
dicarboxylate--CoA ligase PimA; PimA, a member of a large family of acyl-CoA ligases, is found ... |
7-511 |
8.75e-69 |
|
dicarboxylate--CoA ligase PimA; PimA, a member of a large family of acyl-CoA ligases, is found in a characteristic operon pimFABCDE for the metabolism of pimelate and related compounds. It is found, so far, in Bradyrhizobium japonicum and several strains of Rhodopseudomonas palustris. PimA from R. palustris was shown to be active as a CoA ligase for C(7) to C(14) dicarboxylates and fatty acids.
Pssm-ID: 132249 [Multi-domain] Cd Length: 541 Bit Score: 229.85 E-value: 8.75e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 7 LPETFN--AADYfvdrniregrGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAI 84
Cdd:TIGR03205 23 LPDLLSkaAADY----------GPRPALEFRDRPITYTELEAMAETAAAALLRAGYGKDASVALYLGNTPDHPINFFGAL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 85 KIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEelYSKFEPIKdkLRFLK-----HIIVV---------NGQAPAGT-- 148
Cdd:TIGR03205 93 KAGARVVHLSPLDGERALSHKLSDSGARLLITSD--LAALLPMA--LKFLEkglldRLIVCeddnwgkvgTPQAPIPAdp 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 149 --ISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETY---GKQVL---GIQEHDICfsAAK 220
Cdd:TIGR03205 169 riVTYADFVKGAAAPAEWPAVTPDDVALLQYTGGTTGLPKGAMLTHGNLTSAVSIYdvwGKPSRatrGDVERVIC--VLP 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 221 LFFAYGLGNGLYFPFSVGATSVLMPVRPVpASVYETIARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALP 296
Cdd:TIGR03205 247 LFHIYALTVILLRSLRRGDLISLHQRFDV-AAVFRDIEEKRATVFPGVPTMWIALANdpslEKRDLSSLATIGSGGAPLP 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 297 ADIFKRWQQRHGVEIIDGIGSTELSHIFISNLP-GKAKPGSSGKVVPGYDARIV--DENFQDIPVGEVGTLLIKGDSAAA 373
Cdd:TIGR03205 326 VEVANFFERKTGLKLKSGWGMTETCSPGTGHPPeGPDKPGSIGLMLPGIELDVVslDDPTKVLPPGEVGELRIRGPNVTR 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 374 AYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVK 453
Cdd:TIGR03205 406 GYWNRPEESAEAFVGDRFLTGDIGYMDTDGYFFLVDRKKDMIISGGFNVYPQMIEQAIYEHPGVQEVIVIGIPDQYRGEA 485
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 454 PKAFVVLKRGYAPDDQlkKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:TIGR03205 486 AKAFVKLRPGAKPFSL--DELRAFLAGKLGKHELPVAVEFVDELPRTPVGKLSRHELR 541
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
29-511 |
1.55e-65 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 221.57 E-value: 1.55e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PRK07786 33 APALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSD 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGT 188
Cdd:PRK07786 113 CGAHVVVTEAALAPVATAVRDIVPLLSTVVVAGGSSDDSVLGYEDLLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGA 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 189 IHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFpFSVGATSVLMPVRPV-PASVYETIARYRPTLYFG 267
Cdd:PRK07786 193 VLTHANLTGQAMTCLRTNGADINSDVGFVGVPLFHIAGIGSMLPG-LLLGAPTVIYPLGAFdPGQLLDVLEAEKVTGIFL 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 268 VPTLYGSMLQEEGSlDGVRL---CVSAGEALPADIFKR--WQQRHGVEIIDGIGSTELSHIfISNLPGK---AKPGSSGK 339
Cdd:PRK07786 272 VPAQWQAVCAEQQA-RPRDLalrVLSWGAAPASDTLLRqmAATFPEAQILAAFGQTEMSPV-TCMLLGEdaiRKLGSVGK 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 340 VVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGG 419
Cdd:PRK07786 350 VIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAFAGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGG 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 420 IWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyAPDDQLK-KDLKEYVKKTLAHYKYPRWIEFVDDLP 498
Cdd:PRK07786 430 ENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVR---NDDAALTlEDLAEFLTDRLARYKHPKALEIVDALP 506
|
490
....*....|...
gi 700405679 499 KTATGKLKRFELR 511
Cdd:PRK07786 507 RNPAGKVLKTELR 519
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
29-511 |
7.12e-65 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 219.89 E-value: 7.12e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PRK07059 39 RPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKD 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEELYSKFEPIKDKLRfLKHIIV--------------------VNGQAPA----GTISF-DSFVAGASDQLE 163
Cdd:PRK07059 119 SGAEAIVVLENFATTVQQVLAKTA-VKHVVVasmgdllgfkghivnfvvrrVKKMVPAwslpGHVRFnDALAEGARQTFK 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 164 AAPITPDDACFWLYSSGSTGQPKGTIhLQHDMVYA----AETYGKQVLGIQEHD-----ICfsAAKLFFAYGLGNGLYFP 234
Cdd:PRK07059 198 PVKLGPDDVAFLQYTGGTTGVSKGAT-LLHRNIVAnvlqMEAWLQPAFEKKPRPdqlnfVC--ALPLYHIFALTVCGLLG 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 235 FSVGATSVLMP-VRPVPASVYEtIARYRPTLYFGVPTLYGSMLQEEG--SLDGVRLCVS--AGEALPADIFKRWQQRHGV 309
Cdd:PRK07059 275 MRTGGRNILIPnPRDIPGFIKE-LKKYQVHIFPAVNTLYNALLNNPDfdKLDFSKLIVAngGGMAVQRPVAERWLEMTGC 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 310 EIIDGIGSTELSHIFISN-LPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLG 388
Cdd:PRK07059 354 PITEGYGLSETSPVATCNpVDATEFSGTIGLPLPSTEVSIRDDDGNDLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTA 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 389 D-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyapD 467
Cdd:PRK07059 434 DgFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFVVKK-----D 508
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 700405679 468 DQL-KKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK07059 509 PALtEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
27-511 |
7.38e-65 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 218.78 E-value: 7.38e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNA-----FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKD 101
Cdd:PRK08008 21 GHKTALIFESSGgvvrrYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREE 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 102 YLYFLNDSRAKILIIDEELYSKFEPIKDKLRF-LKHIIVVNGQAPA--GTISFDSFVAGASDQL-EAAPITPDDACFWLY 177
Cdd:PRK08008 101 SAWILQNSQASLLVTSAQFYPMYRQIQQEDATpLRHICLTRVALPAddGVSSFTQLKAQQPATLcYAPPLSTDDTAEILF 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLmpVRPVPASVY-ET 256
Cdd:PRK08008 181 TSGTTSRPKGVV-ITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAGATFVL--LEKYSARAFwGQ 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVP----TLygsMLQEEGSLDG---VR-----LCVSAGEAlpaDIFkrwQQRHGVEIIDGIGSTELSHIF 324
Cdd:PRK08008 258 VCKYRATITECIPmmirTL---MVQPPSANDRqhcLRevmfyLNLSDQEK---DAF---EERFGVRLLTSYGMTETIVGI 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPGKAK--PgSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKG---DSAAAAYWNKHQKTKSTMLGD-WVNTDDKYA 398
Cdd:PRK08008 329 IGDRPGDKRrwP-SIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGvpgKTIFKEYYLDPKATAKVLEADgWLHTGDTGY 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 399 VDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGyapdDQLKKD-LKEY 477
Cdd:PRK08008 408 VDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEG----ETLSEEeFFAF 483
|
490 500 510
....*....|....*....|....*....|....
gi 700405679 478 VKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK08008 484 CEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
40-507 |
1.78e-64 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 215.42 E-value: 1.78e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:cd05935 3 TYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGSE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 LyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpDDACFWLYSSGSTGQPKGTIHlQHDMVYAA 199
Cdd:cd05935 83 L-------------------------------------------------DDLALIPYTSGTTGLPKGCMH-THFSAAAN 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 200 ETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMpVRPVPASVYETIARYRPTLYFGVPTLYGSMLQ-- 277
Cdd:cd05935 113 ALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLM-ARWDRETALELIEKYKVTFWTNIPTMLVDLLAtp 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 278 --EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVD-ENFQ 354
Cdd:cd05935 192 efKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTETMSQTHTNPPLRPKLQCLGIP*FGVDARVIDiETGR 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 355 DIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD----WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEAC 430
Cdd:cd05935 272 ELPPNEVGEIVVRGPQIFKGYWNRPEETEESFIEIkgrrFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAK 351
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 700405679 431 LIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd05935 352 LYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKVT-EEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILW 427
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
28-510 |
1.45e-63 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 213.54 E-value: 1.45e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNTlmqPKDYL-Y 104
Cdd:cd05930 2 DAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAayVPLDPSY---PAERLaY 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQ 184
Cdd:cd05930 79 ILEDSGAKLVLTD---------------------------------------------------PDDLAYVIYTSGSTGK 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGT-------IHLQHDMVyaaetygkQVLGIQEHDICFSAAKLFFAYGLGNgLYFPFSVGATSVLMP--VRPVPASVYE 255
Cdd:cd05930 108 PKGVmvehrglVNLLLWMQ--------EAYPLTPGDRVLQFTSFSFDVSVWE-IFGALLAGATLVVLPeeVRKDPEALAD 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGSMLQE--EGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELShIFIS--NLPG 330
Cdd:cd05930 179 LLAEEGITVLHLTPSLLRLLLQEleLAALPSLRLVLVGGEALPPDLVRRWRELLpGARLVNLYGPTEAT-VDATyyRVPP 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 KAKPGSS---GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVN-------TDDKYAVD 400
Cdd:cd05930 258 DDEEDGRvpiGRPIPNTRVYVLDENLRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFGpgermyrTGDLVRWL 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 401 EEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATDDKNLVkpkAFVVLKRGYAPDdqlKKDLKEY 477
Cdd:cd05930 338 PDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVareDGDGEKRLV---AYVVPDEGGELD---EEELRAH 411
|
490 500 510
....*....|....*....|....*....|...
gi 700405679 478 VKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd05930 412 LAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
31-511 |
6.42e-62 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 212.33 E-value: 6.42e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNA--FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PRK12583 36 ALVVRHQAlrYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQ 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILI----------------IDEELYS--KFEPIKDKLRFLKHIIVVNGQAPAGTISFDSFVA---GASDQ-LEA-- 164
Cdd:PRK12583 116 SGVRWVIcadafktsdyhamlqeLLPGLAEgqPGALACERLPELRGVVSLAPAPPPGFLAWHELQArgeTVSREaLAErq 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 165 APITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLM 244
Cdd:PRK12583 196 ASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVA-ESLGLTEHDRLCVPVPLYHCFGMVLANLGCMTVGACLVYP 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 245 PVRPVPASVYETIARYRPTLYFGVPTLYGSMLQ--EEGSLD--GVRLCVSAGEALPADIFKR-WQQRHGVEIIDGIGSTE 319
Cdd:PRK12583 275 NEAFDPLATLQAVEEERCTALYGVPTMFIAELDhpQRGNFDlsSLRTGIMAGAPCPIEVMRRvMDEMHMAEVQIAYGMTE 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 320 LSHIfiSNLPGKAKP-----GSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNT 393
Cdd:PRK12583 355 TSPV--SLQTTAADDlerrvETVGRTQPHLEVKVVDPDGATVPRGEIGELCTRGYSVMKGYWNNPEATAESIDEDgWMHT 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 394 DDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkD 473
Cdd:PRK12583 433 GDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEE---E 509
|
490 500 510
....*....|....*....|....*....|....*...
gi 700405679 474 LKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK12583 510 LREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMR 547
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
64-511 |
1.08e-61 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 211.55 E-value: 1.08e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 64 NRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRfLKHIIV---- 139
Cdd:PRK05677 76 DRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTG-VKHVIVteva 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 140 -----------------VNGQAPA----GTISF-DSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQHDMVY 197
Cdd:PRK05677 155 dmlpplkrllinavvkhVKKMVPAyhlpQAVKFnDALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAM-LTHRNLV 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 198 AAETYGKQVLGIQEHDIC---FSAAKLFFAYGLGNGLYFPFSVGATSVLMP-VRPVPASVYEtIARYRPTLYFGVPTLYG 273
Cdd:PRK05677 234 ANMLQCRALMGSNLNEGCeilIAPLPLYHIYAFTFHCMAMMLIGNHNILISnPRDLPAMVKE-LGKWKFSGFVGLNTLFV 312
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 274 SMLQEEG--SLD--GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIV 349
Cdd:PRK05677 313 ALCNNEAfrKLDfsALKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVI 392
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 350 DENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVE 428
Cdd:PRK05677 393 DDDGNELPLGEVGELCVKGPQVMKGYWQRPEATDEILDSDgWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELE 472
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 429 ACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRF 508
Cdd:PRK05677 473 DVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLT---KEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRR 549
|
...
gi 700405679 509 ELR 511
Cdd:PRK05677 550 ELR 552
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
57-511 |
2.79e-61 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 210.30 E-value: 2.79e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 57 GLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRfLKH 136
Cdd:PRK08974 68 GLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTP-VKH 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 137 IIVVN-GQ---APAGT---------------------ISF-DSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIH 190
Cdd:PRK08974 147 VILTRmGDqlsTAKGTlvnfvvkyikrlvpkyhlpdaISFrSALHKGRRMQYVKPELVPEDLAFLQYTGGTTGVAKGAML 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 LQHDMV----YAAETYGKqvLGIQEHDICFSAAKLFFAYGLG-NGLYFpFSVGATSVLM--PvRPVPASVYEtIARYRPT 263
Cdd:PRK08974 227 THRNMLanleQAKAAYGP--LLHPGKELVVTALPLYHIFALTvNCLLF-IELGGQNLLItnP-RDIPGFVKE-LKKYPFT 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQEEG--SLD--GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNlPGKAK--PGSS 337
Cdd:PRK08974 302 AITGVNTLFNALLNNEEfqELDfsSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSVN-PYDLDyySGSI 380
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK08974 381 GLPVPSTEIKLVDDDGNEVPPGEPGELWVKGPQVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILV 460
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyapDDQLKKD-LKEYVKKTLAHYKYPRWIEFVDD 496
Cdd:PRK08974 461 SGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKK-----DPSLTEEeLITHCRRHLTGYKVPKLVEFRDE 535
|
490
....*....|....*
gi 700405679 497 LPKTATGKLKRFELR 511
Cdd:PRK08974 536 LPKSNVGKILRRELR 550
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
40-512 |
4.95e-60 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 208.66 E-value: 4.95e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIG-AVPIclNTLMQPKDYLYFLNDSRAKILII-- 116
Cdd:PRK07529 60 TYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAGiANPI--NPLLEPEQIAELLRAAGAKVLVTlg 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 ---DEELYSKFEPIKDKLRFLKHIIVVNG-----------------QAPAGTISFDSFVAGAS-DQLEAA-PITPDDACF 174
Cdd:PRK07529 138 pfpGTDIWQKVAEVLAALPELRTVVEVDLarylpgpkrlavplirrKAHARILDFDAELARQPgDRLFSGrPIGPDDVAA 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 175 WLYSSGSTGQPKGTIHLQHDMVYAAETyGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLmpvrPVPA--- 251
Cdd:PRK07529 218 YFHTGGTTGMPKLAQHTHGNEVANAWL-GALLLGLGPGDTVFCGLPLFHVNALLVTGLAPLARGAHVVL----ATPQgyr 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 252 --SVY----ETIARYRPTLYFGVPTLYGSMLQ---EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSH 322
Cdd:PRK07529 293 gpGVIanfwKIVERYRINFLSGVPTVYAALLQvpvDGHDISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATC 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 323 IFISNLP-GKAKPGSSGKVVPGYDARIV--DEN---FQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDK 396
Cdd:PRK07529 373 VSSVNPPdGERRIGSVGLRLPYQRVRVVilDDAgryLRDCAVDEVGVLCIAGPNVFSGYLEAAHNKGLWLEDGWLNTGDL 452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 397 YAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGY-APDDQLKKDLK 475
Cdd:PRK07529 453 GRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGAsATEAELLAFAR 532
|
490 500 510
....*....|....*....|....*....|....*..
gi 700405679 476 EYVKKTLAHykyPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK07529 533 DHIAERAAV---PKHVRILDALPKTAVGKIFKPALRR 566
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
40-507 |
8.33e-60 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 206.81 E-value: 8.33e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:PRK06710 51 TFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLDL 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 LYSKFEPIKDKLRfLKHIIV-------------------------VNGQAPAGTISFDSFVAGASDQLEAAPITPD-DAC 173
Cdd:PRK06710 131 VFPRVTNVQSATK-IEHVIVtriadflpfpknllypfvqkkqsnlVVKVSESETIHLWNSVEKEVNTGVEVPCDPEnDLA 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 174 FWLYSSGSTGQPKGTIhLQHDMVYAAETYGKQVL--GIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPA 251
Cdd:PRK06710 210 LLQYTGGTTGFPKGVM-LTHKNLVSNTLMGVQWLynCKEGEEVVLGVLPFFHVYGMTAVMNLSIMQGYKMVLIPKFDMKM 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 252 sVYETIARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISN 327
Cdd:PRK06710 289 -VFEAIKKHKVTLFPGAPTIYIALLNspllKEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSPVTHSN 367
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 -LPGKAKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYF 405
Cdd:PRK06710 368 fLWEKRVPGSIGVPWPDTEAMIMSlETGEALPPGEIGEIVVKGPQIMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFF 447
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHY 485
Cdd:PRK06710 448 YVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEE---ELNQFARKYLAAY 524
|
490 500
....*....|....*....|..
gi 700405679 486 KYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK06710 525 KVPKVYEFRDELPKTTVGKILR 546
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
30-512 |
2.10e-59 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 205.16 E-value: 2.10e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 30 TAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDS 109
Cdd:PRK07788 66 AALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVAARE 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 110 RAKILIIDEELYSKFEPIKDKLRFLkHIIVVN----GQAPAGTISFDSFVAGASDqlEAAPITPDDACFWLYSSGSTGQP 185
Cdd:PRK07788 146 GVKALVYDDEFTDLLSALPPDLGRL-RAWGGNpdddEPSGSTDETLDDLIAGSST--APLPKPPKPGGIVILTSGTTGTP 222
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTIHlqhdmvyaAETYGKQVLG-------IQEHDICFSAAKLFFAYGLGNgLYFPFSVGATSVLMPvRPVPASVYETIA 258
Cdd:PRK07788 223 KGAPR--------PEPSPLAPLAgllsrvpFRAGETTLLPAPMFHATGWAH-LTLAMALGSTVVLRR-RFDPEATLEDIA 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 259 RYRPTLYFGVPTLYGSMLQEEGSLDG------VRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKA 332
Cdd:PRK07788 293 KHKATALVVVPVMLSRILDLGPEVLAkydtssLKIIFVSGSALSPELATRALEAFGPVLYNLYGSTEVAFATIATPEDLA 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 K-PGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWN-KHQKTKSTMLgdwvNTDDKYAVDEEGYFYYIGR 410
Cdd:PRK07788 373 EaPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGYTDgRDKQIIDGLL----SSGDVGYFDEDGLLFVDGR 448
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 411 SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRW 490
Cdd:PRK07788 449 DDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALD---EDAIKDYVRDNLARYKVPRD 525
|
490 500
....*....|....*....|..
gi 700405679 491 IEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK07788 526 VVFLDELPRNPTGKVLKRELRE 547
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
169-511 |
2.21e-59 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 199.81 E-value: 2.21e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRP 248
Cdd:cd05917 1 PDDVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGER-LGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFPSPSF 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPASVYETIARYRPTLYFGVPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQQRHGV-EIIDGIGSTELSHI 323
Cdd:cd05917 80 DPLAVLEAIEKEKCTALHGVPTMFIAELehpdFDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMkDVTIAYGMTETSPV 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 324 FISNLPGKA---KPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYA 398
Cdd:cd05917 160 STQTRTDDSiekRVNTVGRIMPHTEAKIVDpEGGIVPPVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDgWLHTGDLAV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 399 VDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYV 478
Cdd:cd05917 240 MDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAELTEE---DIKAYC 316
|
330 340 350
....*....|....*....|....*....|...
gi 700405679 479 KKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05917 317 KGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
46-511 |
1.79e-58 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 200.36 E-value: 1.79e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 46 AGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA------VPicLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:cd05922 1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGrlglvfVP--LNPTLKESVLRYLVADAGGRIVLADAG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 LYSKFEPIKDKLRFLKHIIVVNGqapagtisfdsfVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQH-DMVYA 198
Cdd:cd05922 79 AADRLRDALPASPDPGTVLDADG------------IRAARASAPAHEVSHEDLALLLYTSGSTGSPKL-VRLSHqNLLAN 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 199 AETYgKQVLGIQEHDICFSAAKLFFAYGLgNGLYFPFSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYG---SM 275
Cdd:cd05922 146 ARSI-AEYLGITADDRALTVLPLSYDYGL-SVLNTHLLRGATLVLTNDGVLDDAFWEDLREHGATGLAGVPSTYAmltRL 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 276 LQEEGSLDGVRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTELSHiFISNLPG---KAKPGSSGKVVPGYDARIVDE 351
Cdd:cd05922 224 GFDPAKLPSLRYLTQAGGRLPQETIARLRELlPGAQVYVMYGQTEATR-RMTYLPPeriLEKPGSIGLAIPGGEFEILDD 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 352 NFQDIPVGEVGTLLIKGDSAAAAYWNKHQ-KTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEAC 430
Cdd:cd05922 303 DGTPTPPGEPGEIVHRGPNVMKGYWNDPPyRRKEGRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAA 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 431 LIEHPAVLECAVVGAtDDKNLVKPKAFVVLKRGYAPDDQLKkdlkeYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd05922 383 ARSIGLIIEAAAVGL-PDPLGEKLALFVTAPDKIDPKDVLR-----SLAERLPPYKVPATVRVVDELPLTASGKVDYAAL 456
|
.
gi 700405679 511 R 511
Cdd:cd05922 457 R 457
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
10-512 |
2.42e-58 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 203.49 E-value: 2.42e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 10 TFNAADYFVDRNIREGRGQKTAILCRDN----AFTYAQVQAGMNKVGNALKGLGIEMENRVALLL-LDSEFYPlSFFGAI 84
Cdd:cd05968 59 RMNIVEQLLDKWLADTRTRPALRWEGEDgtsrTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLpMIPEIVP-AFLAVA 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 85 KIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPI--KDKLRF-------LKHIIVVNG------QAPAGTI 149
Cdd:cd05968 138 RIGGIVVPIFSGFGKEAAATRLQDAEAKALITADGFTRRGREVnlKEEADKacaqcptVEKVVVVRHlgndftPAKGRDL 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 150 SFDSFVAGASDqlEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAetygkqvlgiqehdicfsAAKLFFAYGLGN 229
Cdd:cd05968 218 SYDEEKETAGD--GAERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLKA------------------AQDMYFQFDLKP 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 230 G-----------------LYFPFSVGATSVL---MPVRPVPASVYETIARYRPTlYFGV-PTL------YGSMLQEEGSL 282
Cdd:cd05968 278 GdlltwftdlgwmmgpwlIFGGLILGATMVLydgAPDHPKADRLWRMVEDHEIT-HLGLsPTLiralkpRGDAPVNAHDL 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 283 DGVRLCVSAGEALPADIFkRWQQRH----GVEIIDGIGSTELSH-IFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIP 357
Cdd:cd05968 357 SSLRVLGSTGEPWNPEPW-NWLFETvgkgRNPIINYSGGTEISGgILGNVLIKPIKPSSFNGPVPGMKADVLDESGKPAR 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 358 vGEVGTLLI--------KG-----DSAAAAYWNKHQktkstmlGDWVNTDDKYaVDEEGYFYYIGRSNDMLKVGGIWVSP 424
Cdd:cd05968 436 -PEVGELVLlapwpgmtRGfwrdeDRYLETYWSRFD-------NVWVHGDFAY-YDEEGYFYILGRSDDTINVAGKRVGP 506
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 425 VEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:cd05968 507 AEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAK 586
|
....*...
gi 700405679 505 LKRFELRA 512
Cdd:cd05968 587 VMRRVIRA 594
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
39-512 |
4.78e-58 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 198.56 E-value: 4.78e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDylyflndsrakiliide 118
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDD----------------- 63
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 elyskfepIKDKLRFLKHIIvvngqapagtisfdsfvaGASDQLEAApitpDDACFWLYSSGSTGQPKGTIHLQ--HDMV 196
Cdd:cd05974 64 --------LRDRVDRGGAVY------------------AAVDENTHA----DDPMLLYFTSGTTSKPKLVEHTHrsYPVG 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 197 YAAETYGkqvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLM-PVRPVPASVYETIARYRPTLYFGVPTLYGSM 275
Cdd:cd05974 114 HLSTMYW---IGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFnYARFDAKRVLAALVRYGVTTLCAPPTVWRML 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 276 LQEE-GSLD-GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELShIFISNLPGK-AKPGSSGKVVPGYDARIVDEN 352
Cdd:cd05974 191 IQQDlASFDvKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETT-ALVGNSPGQpVKAGSMGRPLPGYRVALLDPD 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 353 FQDIPVGEVgTLLIKGDSAA---AAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEA 429
Cdd:cd05974 270 GAPATEGEV-ALDLGDTRPVglmKGYAGDPDKTAHAMRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELES 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 430 CLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVdDLPKTATGKLKRFE 509
Cdd:cd05974 349 VLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVE 427
|
...
gi 700405679 510 LRA 512
Cdd:cd05974 428 LRR 430
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
29-511 |
6.10e-58 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 201.13 E-value: 6.10e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILcrDN---AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLD-SEFyPLSFFGAIKIGAVPICLNTLMQPKDYLY 104
Cdd:PRK06087 39 KIAVV--DNhgaSYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGwCEF-TIIYLACLKVGAVSVPLLPSWREAELVW 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILI-------IDEElySKFEPIKDKLRFLKHIIVVNGQAPA-GTISFDSFVAGASDQLEAAPITPDDACFWL 176
Cdd:PRK06087 116 VLNKCQAKMFFaptlfkqTRPV--DLILPLQNQLPQLQQIVGVDKLAPAtSSLSLSQIIADYEPLTTAITTHGDELAAVL 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvRPVPASVYET 256
Cdd:PRK06087 194 FTSGTEGLPKGVM-LTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLD-IFTPDACLAL 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFG-VPTLYG--SMLQEEGS-LDGVRLCVSAGEALPADIFKRWQQrHGVEIIDGIGSTELS-HIFISnlPGK 331
Cdd:PRK06087 272 LEQQRCTCMLGaTPFIYDllNLLEKQPAdLSALRFFLCGGTTIPKKVARECQQ-RGIKLLSVYGSTESSpHAVVN--LDD 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 332 AKP---GSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYY 407
Cdd:PRK06087 349 PLSrfmHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELTARALDEEgWYYSGDLCRMDEAGYIKI 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 408 IGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyAPDDQLK-KDLKEYV-KKTLAHY 485
Cdd:PRK06087 429 TGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLK---APHHSLTlEEVVAFFsRKRVAKY 505
|
490 500
....*....|....*....|....*.
gi 700405679 486 KYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK06087 506 KYPEHIVVIDKLPRTASGKIQKFLLR 531
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
38-511 |
1.61e-57 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 198.97 E-value: 1.61e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:PRK08276 11 VVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVS 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKFEPIKDKLRF-LKHIIVVNGQAPaGTISFDSFVAGASDqleaAPItPDDACFW--LYSSGSTGQPKGTIH-LQH 193
Cdd:PRK08276 91 AALADTAAELAAELPAgVPLLLVVAGPVP-GFRSYEEALAAQPD----TPI-ADETAGAdmLYSSGTTGRPKGIKRpLPG 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 dmvyaaetygkqvLGIQEHDICFSAAKLFFAYGLGNGLY-------------FPFSV---GATSVLMPvRPVPASVYETI 257
Cdd:PRK08276 165 -------------LDPDEAPGMMLALLGFGMYGGPDSVYlspaplyhtaplrFGMSAlalGGTVVVME-KFDAEEALALI 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPT-LYFgVPTLYGSMLQ--EE--GSLD--GVRLCVSAGEALPADIfKR-----WqqrhGVEIIDGIGSTELSHI-F 324
Cdd:PRK08276 231 ERYRVThSQL-VPTMFVRMLKlpEEvrARYDvsSLRVAIHAAAPCPVEV-KRamidwW----GPIIHEYYASSEGGGVtV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPGKAKPGSSGKVVPGyDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLG-DWVNTDDKYAVDEEG 403
Cdd:PRK08276 305 ITSEDWLAHPGSVGKAVLG-EVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARNPhGWVTVGDVGYLDEDG 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 404 YFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLA 483
Cdd:PRK08276 384 YLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLA 463
|
490 500
....*....|....*....|....*...
gi 700405679 484 HYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK08276 464 HYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
38-512 |
1.74e-57 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 197.22 E-value: 1.74e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EElYSKFEPIKDklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKGTIHLQHDMVY 197
Cdd:cd05903 81 ER-FRQFDPAAM---------------------------------------PDAVALLLFTSGTTGEPKGVMHSHNTLSA 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 198 AAETYgKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPvPASVYETIARYRPTLYFGVPTLYGSMLQ 277
Cdd:cd05903 121 SIRQY-AERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWD-PDKALALMREHGVTFMMGATPFLTDLLN 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 278 --EEGS--LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKP--GSSGKVVPGYDARIVDE 351
Cdd:cd05903 199 avEEAGepLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPEDRrlYTDGRPLPGVEIKVVDD 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 352 NFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACL 431
Cdd:cd05903 279 TGATLAPGVEGELLSRGPSVFLGYLDRPDLTADAAPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLL 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 432 IEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYV-KKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd05903 359 LGHPGVIEAAVVALPDERLGERACAVVVTKSGALLT---FDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRL 435
|
..
gi 700405679 511 RA 512
Cdd:cd05903 436 RE 437
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
40-511 |
4.48e-57 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 199.33 E-value: 4.48e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:PRK08751 52 TYREADQLVEQFAAYLLGeLQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVID 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFE------PIK--------DKLRFLKHIIV------VNGQAP----AGTISF-DSFVAGASDQLEAAPITPDDAC 173
Cdd:PRK08751 132 NFGTTVQqviadtPVKqvittglgDMLGFPKAALVnfvvkyVKKLVPeyriNGAIRFrEALALGRKHSMPTLQIEPDDIA 211
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 174 FWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLG---IQE-HDICFSAAKLFFAYGL-GNGLYFPFSVGATSVLMPVRP 248
Cdd:PRK08751 212 FLQYTGGTTGVAKGAMLTHRNLVANMQQAHQWLAGtgkLEEgCEVVITALPLYHIFALtANGLVFMKIGGCNHLISNPRD 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPASVYEtIARYRPTLYFGVPTLYGSMLQEEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELS-HI 323
Cdd:PRK08751 292 MPGFVKE-LKKTRFTAFTGVNTLFNGLLNTPGfdqiDFSSLKMTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSpAA 370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 324 FISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEE 402
Cdd:PRK08751 371 CINPLTLKEYNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADgWLHTGDIARMDEQ 450
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 403 GYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyapDDQLK-KDLKEYVKKT 481
Cdd:PRK08751 451 GFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKK-----DPALTaEDVKAHARAN 525
|
490 500 510
....*....|....*....|....*....|
gi 700405679 482 LAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK08751 526 LTGYKQPRIIEFRKELPKTNVGKILRRELR 555
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
1-507 |
1.73e-56 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 198.40 E-value: 1.73e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 1 MHVELNLPETFNAADYFVDRNIRegRGQKTAILCRDN----AFTYAQVQAGMNKVGNALKGLGIE--------MENRVAL 68
Cdd:COG1022 1 MSEFSDVPPADTLPDLLRRRAAR--FPDRVALREKEDgiwqSLTWAEFAERVRALAAGLLALGVKpgdrvailSDNRPEW 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 69 LLLDsefyplsfFGAIKIGAVPICL-NTLMqPKDYLYFLNDSRAKILII-DEELYSKFEPIKDKLRFLKHIIVVNGQAPA 146
Cdd:COG1022 79 VIAD--------LAILAAGAVTVPIyPTSS-AEEVAYILNDSGAKVLFVeDQEQLDKLLEVRDELPSLRHIVVLDPRGLR 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 147 G---TISFDSFVA-GAS----DQLEA--APITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYgKQVLGIQEHDICF 216
Cdd:COG1022 150 DdprLLSLDELLAlGREvadpAELEArrAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARAL-LERLPLGPGDRTL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 217 S-------AAKLFFAYGLGNG--LYFPfsvgatsvlmpvrPVPASVYETIARYRPTLYFGVPTLYGSMLQ------EEGS 281
Cdd:COG1022 229 SflplahvFERTVSYYALAAGatVAFA-------------ESPDTLAEDLREVKPTFMLAVPRVWEKVYAgiqakaEEAG 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 282 ---------------------LDG-----------------------------VRLCVSAGEALPADIFkRWQQRHGVEI 311
Cdd:COG1022 296 glkrklfrwalavgrryararLAGkspslllrlkhaladklvfsklrealggrLRFAVSGGAALGPELA-RFFRALGIPV 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 312 IDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIvdenfqdipvGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-W 390
Cdd:COG1022 375 LEGYGLTETSPVITVNRPGDNRIGTVGPPLPGVEVKI----------AEDGEILVRGPNVMKGYYKNPEATAEAFDADgW 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 391 VNTDDKYAVDEEGYFYYIGRSNDMLKV-GGIWVSPVEVEACLIEHPAVLECAVVGatDDKN-LVkpkAFVVL-------- 460
Cdd:COG1022 445 LHTGDIGELDEDGFLRITGRKKDLIVTsGGKNVAPQPIENALKASPLIEQAVVVG--DGRPfLA---ALIVPdfealgew 519
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 461 --KRGYAPDD--------QLKKDLKEYVKKTLAHykYPRW-----IEFvddLPK---------TATGKLKR 507
Cdd:COG1022 520 aeENGLPYTSyaelaqdpEVRALIQEEVDRANAG--LSRAeqikrFRL---LPKeftiengelTPTLKLKR 585
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
4-512 |
2.64e-56 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 198.44 E-value: 2.64e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 4 ELNLpeTFNAadyfVDRNIrEGRGQKTAIL------CRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLldsefyP 77
Cdd:PRK00174 65 ELNV--SYNC----LDRHL-KTRGDKVAIIwegddpGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYM------P 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 78 LSFFGAI------KIGAV---------PICLNTLmqpkdylyfLNDSRAKILIIDEELY--SKFEPIKD-------KLRF 133
Cdd:PRK00174 132 MIPEAAVamlacaRIGAVhsvvfggfsAEALADR---------IIDAGAKLVITADEGVrgGKPIPLKAnvdealaNCPS 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 134 LKHIIVV--NGQAPAGT----ISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtihLQHD----MVYAAETYg 203
Cdd:PRK00174 203 VEKVIVVrrTGGDVDWVegrdLWWHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKG---VLHTtggyLVYAAMTM- 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 204 KQVLGIQEHDICFSAAKL-------FFAYGlgnglyfPFSVGATSVL---MPVRPVPASVYETIARYRPTLYFGVPTL-- 271
Cdd:PRK00174 279 KYVFDYKDGDVYWCTADVgwvtghsYIVYG-------PLANGATTLMfegVPNYPDPGRFWEVIDKHKVTIFYTAPTAir 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 ----YGSMLQEEGSLDGVRLCVSAGEALPADIFkRWQQRH-GVE---IIDGIGSTELSHIFISNLPG--KAKPGSSGKVV 341
Cdd:PRK00174 352 almkEGDEHPKKYDLSSLRLLGSVGEPINPEAW-EWYYKVvGGErcpIVDTWWQTETGGIMITPLPGatPLKPGSATRPL 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 342 PGYDARIVDENFQDIPVGEVGTLLIKGD--SAAAAYWNKHQKTKSTMlgdWVNTDDKY-----AV-DEEGYFYYIGRSND 413
Cdd:PRK00174 431 PGIQPAVVDEEGNPLEGGEGGNLVIKDPwpGMMRTIYGDHERFVKTY---FSTFKGMYftgdgARrDEDGYYWITGRVDD 507
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 414 MLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDknlVK---PKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRW 490
Cdd:PRK00174 508 VLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDD---IKgqgIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDV 584
|
570 580
....*....|....*....|..
gi 700405679 491 IEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK00174 585 IQFAPGLPKTRSGKIMRRILRK 606
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
36-511 |
4.01e-56 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 195.68 E-value: 4.01e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:PRK13391 22 GEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELYSKFEPIKDKLRFLKHIIVVNGqaPAGTISFDSFvAGASDQLEAAPItpDDACF---WLYSSGSTGQPKGTI--- 189
Cdd:PRK13391 102 TSAAKLDVARALLKQCPGVRHRLVLDG--DGELEGFVGY-AEAVAGLPATPI--ADESLgtdMLYSSGTTGRPKGIKrpl 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 -HLQHDMVYAAETYGKQVLGIQEHDICFSAAKLF-----FAYGLGNglyfpfSVGATSVLMPvRPVPASVYETIARYRPT 263
Cdd:PRK13391 177 pEQPPDTPLPLTAFLQRLWGFRSDMVYLSPAPLYhsapqRAVMLVI------RLGGTVIVME-HFDAEQYLALIEEYGVT 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQ--EEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTE-LSHIFISNLPGKAKPGS 336
Cdd:PRK13391 250 HTQLVPTMFSRMLKlpEEVrdkyDLSSLEVAIHAAAPCPPQVKEQMIDWWGPIIHEYYAATEgLGFTACDSEEWLAHPGT 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 SGKVVPGyDARIVDENFQDIPVGEVGTLLIKGDSAAAaYWNKHQKTKSTML--GDWVNTDDKYAVDEEGYFYYIGRSNDM 414
Cdd:PRK13391 330 VGRAMFG-DLHILDDDGAELPPGEPGTIWFEGGRPFE-YLNDPAKTAEARHpdGTWSTVGDIGYVDEDGYLYLTDRAAFM 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 415 LKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFV 494
Cdd:PRK13391 408 IISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFE 487
|
490
....*....|....*..
gi 700405679 495 DDLPKTATGKLKRFELR 511
Cdd:PRK13391 488 DELPRLPTGKLYKRLLR 504
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
40-511 |
5.14e-55 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 193.04 E-value: 5.14e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVITDLT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 LYSKFEPIKDKLRFLKHIIVVNGQA--PAGT----ISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHlQH 193
Cdd:PRK06018 121 FVPILEKIADKLPSVERYVVLTDAAhmPQTTlknaVAYEEWIAEADGDFAWKTFDENTAAGMCYTSGTTGDPKGVLY-SH 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 --DMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPfSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTL 271
Cdd:PRK06018 200 rsNVLHALMANNGDALGTSAADTMLPVVPLFHANSWGIAFSAP-SMGTKLVMPGAKLDGASVYELLDTEKVTFTAGVPTV 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 YGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQrHGVEIIDGIGSTELSHI--------FISNLPGKAK---PGS 336
Cdd:PRK06018 279 WLMLLQymekEGLKLPHLKMVVCGGSAMPRSMIKAFED-MGVEVRHAWGMTEMSPLgtlaalkpPFSKLPGDARldvLQK 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 SGKVVPGYDARIVDENFQDIPV-GEV-GTLLIKGDSAAAAYWnkhqKTKSTMLGD--WVNTDDKYAVDEEGYFYYIGRSN 412
Cdd:PRK06018 358 QGYPPFGVEMKITDDAGKELPWdGKTfGRLKVRGPAVAAAYY----RVDGEILDDdgFFDTGDVATIDAYGYMRITDRSK 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 413 DMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIE 492
Cdd:PRK06018 434 DVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGETAT---REEILKYMDGKIAKWWMPDDVA 510
|
490
....*....|....*....
gi 700405679 493 FVDDLPKTATGKLKRFELR 511
Cdd:PRK06018 511 FVDAIPHTATGKILKTALR 529
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
26-511 |
1.17e-54 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 191.17 E-value: 1.17e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNA----FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKD 101
Cdd:PRK09088 6 RLQPQRLAAVDLAlgrrWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASE 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 102 YLYFLNDSRAKILIIDEELyskfepikdklrflkhiivvngqAPAGTI--SFDSFVAGAsDQLEAA---PITPDDACFWL 176
Cdd:PRK09088 86 LDALLQDAEPRLLLGDDAV-----------------------AAGRTDveDLAAFIASA-DALEPAdtpSIPPERVSLIL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIHLQHDMVYAAETYGkqVLG-IQEHDICFSAAKLFFAYGLGNGLYFPFSVGATsVLMPVRPVPAsvyE 255
Cdd:PRK09088 142 FTSGTSGQPKGVMLSERNLQQTAHNFG--VLGrVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGS-ILVSNGFEPK---R 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARY-RPTL----YFGVPTLyGSMLQEEGSLDGVRL-----CVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIF- 324
Cdd:PRK09088 216 TLGRLgDPALgithYFCVPQM-AQAFRAQPGFDAAALrhltaLFTGGAPHAAEDILGWLDD-GIPMVDGFGMSEAGTVFg 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPG--KAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDE 401
Cdd:PRK09088 294 MSVDCDviRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDgWFRTGDIARRDA 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 402 EGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKT 481
Cdd:PRK09088 374 DGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLD---LERIRSHLSTR 450
|
490 500 510
....*....|....*....|....*....|
gi 700405679 482 LAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK09088 451 LAKYKVPKHLRLVDALPRTASGKLQKARLR 480
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
40-512 |
1.34e-54 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 188.71 E-value: 1.34e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKIliide 118
Cdd:cd05912 3 TFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSiEMI-LLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 elyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpDDACFWLYSSGSTGQPKGTIhLQHDMVYA 198
Cdd:cd05912 77 ---------------------------------------------------DDIATIMYTSGTTGKPKGVQ-QTFGNHWW 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 199 AETYGKQVLGIQEHDICFSAAKLFFAYGLgnglyfpfSVGATSVL--MPVRPV----PASVYETIARYRPTLYFGVPTLY 272
Cdd:cd05912 105 SAIGSALNLGLTEDDNWLCALPLFHISGL--------SILMRSVIygMTVYLVdkfdAEQVLHLINSGKVTIISVVPTML 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 273 GSMLQEEGSL--DGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIFISNLPGKA--KPGSSGKVVPGYDARI 348
Cdd:cd05912 177 QRLLEILGEGypNNLRCILLGGGPAPKPLLEQCKEK-GIPVYQSYGMTETCSQIVTLSPEDAlnKIGSAGKPLFPVELKI 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 349 VDENfqdIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVE 428
Cdd:cd05912 256 EDDG---QPPYEVGEILLKGPNVTKGYLNRPDATEESFENGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIE 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 429 ACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRgyapdDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRF 508
Cdd:cd05912 333 EVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSER-----PISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRH 407
|
....
gi 700405679 509 ELRA 512
Cdd:cd05912 408 ELKQ 411
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
40-512 |
2.11e-54 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 191.51 E-value: 2.11e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:PRK13382 70 TWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIYDEE 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 lyskFEPIKDKlrflkhiiVVNGqAPAGTISF---DSFVAGASDQLEAAPI------TPDDACFWLYSSGSTGQPKGTIH 190
Cdd:PRK13382 150 ----FSATVDR--------ALAD-CPQATRIVawtDEDHDLTVEVLIAAHAgqrpepTGRKGRVILLTSGTTGTPKGARR 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 lqhdmvYAAETYG--KQVLG---IQEHDICFSAAKLFFAYGLGNgLYFPFSVGATsVLMPVRPVPASVYETIARYRPTLY 265
Cdd:PRK13382 217 ------SGPGGIGtlKAILDrtpWRAEEPTVIVAPMFHAWGFSQ-LVLAASLACT-IVTRRRFDPEATLDLIDRHRATGL 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 266 FGVPTLYGSMLQ--EEG----SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNlPG--KAKPGSS 337
Cdd:PRK13382 289 AVVPVMFDRIMDlpAEVrnrySGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIATAT-PAdlRAAPDTA 367
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYwnKHQKTKSTMLGdWVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK13382 368 GRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY--TSGSTKDFHDG-FMASGDVGYLDENGRLFVVGRDDEMIVS 444
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYApddQLKKDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PRK13382 445 GGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGAS---ATPETLKQHVRDNLANYKVPRDIVVLDEL 521
|
490
....*....|....*
gi 700405679 498 PKTATGKLKRFELRA 512
Cdd:PRK13382 522 PRGATGKILRRELQA 536
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
39-511 |
4.03e-54 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 191.03 E-value: 4.03e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPiclNTLM---QPKDYLYFLNDSRAKILI 115
Cdd:PRK13295 56 FTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVL---NPLMpifRERELSFMLKHAESKVLV 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEElYSKF------EPIKDKLRFLKHIIVVNGqapAGTISFDSFVAG-ASDQLEAAPIT-------PDDACFWLYSSGS 181
Cdd:PRK13295 133 VPKT-FRGFdhaamaRRLRPELPALRHVVVVGG---DGADSFEALLITpAWEQEPDAPAIlarlrpgPDDVTQLIYTSGT 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 182 TGQPKGTIHLQHDMVYAAETYGKQvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPvPASVYETIARYR 261
Cdd:PRK13295 209 TGEPKGVMHTANTLMANIVPYAER-LGLGADDVILMASPMAHQTGFMYGLMMPVMLGATAVLQDIWD-PARAAELIRTEG 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 262 PTLYFGVPTLYGSML--QEEGSLD--GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNL--PGKAKPG 335
Cdd:PRK13295 287 VTFTMASTPFLTDLTraVKESGRPvsSLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTLTKLddPDERAST 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 336 SSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGdWVNTDDKYAVDEEGYFYYIGRSNDML 415
Cdd:PRK13295 367 TDGCPLPGVEVRVVDADGAPLPAGQIGRLQVRGCSNFGGYLKRPQLNGTDADG-WFDTGDLARIDADGYIRISGRSKDVI 445
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 416 KVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVK-KTLAHYKYPRWIEFV 494
Cdd:PRK13295 446 IRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLD---FEEMVEFLKaQKVAKQYIPERLVVR 522
|
490
....*....|....*..
gi 700405679 495 DDLPKTATGKLKRFELR 511
Cdd:PRK13295 523 DALPRTPSGKIQKFRLR 539
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
29-512 |
7.59e-54 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 188.63 E-value: 7.59e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PRK03640 18 RTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQLDD 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEELYSKFEPIKdklrflkhiivvngqapagTISFDSFVAGA-SDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK03640 98 AEVKCLITDDDFEAKLIPGI-------------------SVKFAELMNGPkEEAEIQEEFDLDEVATIMYTSGTTGKPKG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIhlqhdmvyaaETYGKQV---------LGIQEHDICFSAAKLFFAYGLgnglyfpfSVGATSVL--MPVRPVPA----S 252
Cdd:PRK03640 159 VI----------QTYGNHWwsavgsalnLGLTEDDCWLAAVPIFHISGL--------SILMRSVIygMRVVLVEKfdaeK 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARYRPTLYFGVPTLYGSMLQ---EEGSLDGVRlCVSAGEAlPADIFKRWQ-QRHGVEIIDGIGSTELSHIfISNL 328
Cdd:PRK03640 221 INKLLQTGGVTIISVVSTMLQRLLErlgEGTYPSSFR-CMLLGGG-PAPKPLLEQcKEKGIPVYQSYGMTETASQ-IVTL 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 329 PGK---AKPGSSGKVVPGYDARIVDeNFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYF 405
Cdd:PRK03640 298 SPEdalTKLGSAGKPLFPCELKIEK-DGVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQDGWFKTGDIGYLDEEGFL 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlkkDLKEYVKKTLAHY 485
Cdd:PRK03640 377 YVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVKSGEVTEE-----ELRHFCEEKLAKY 451
|
490 500
....*....|....*....|....*..
gi 700405679 486 KYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK03640 452 KVPKRFYFVEELPRNASGKLLRHELKQ 478
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
26-511 |
3.42e-53 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 187.39 E-value: 3.42e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAF-TYAQVQAGMNKVGNALKGLGIEMENRVAL--------LLLdsefyplsFFGAIKIGAVPICLNTL 96
Cdd:PRK07514 15 DRDAPFIETPDGLRyTYGDLDAASARLANLLVALGVKPGDRVAVqvekspeaLAL--------YLATLRAGAVFLPLNTA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 97 MQPKDYLYFLNDSRAKILIIDEelySKFEPIKdKLRFLKHIIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWL 176
Cdd:PRK07514 87 YTLAELDYFIGDAEPALVVCDP---ANFAWLS-KIAAAAGAPHVETLDADGTGSLLEAAAAAPDDFETVPRGADDLAAIL 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIhLQHDMVYA-AETYgKQVLGIQEHDICFSAAKLFFAYGL---GNGLYFpfsVGATSVLMPvRPVPAS 252
Cdd:PRK07514 163 YTSGTTGRSKGAM-LSHGNLLSnALTL-VDYWRFTPDDVLIHALPIFHTHGLfvaTNVALL---AGASMIFLP-KFDPDA 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARyrPTLYFGVPTLYGSMLQEEGsLD-----GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTElSHIFISN 327
Cdd:PRK07514 237 VLALMPR--ATVMMGVPTFYTRLLQEPR-LTreaaaHMRLFISGSAPLLAETHREFQERTGHAILERYGMTE-TNMNTSN 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 -LPGKAKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGY 404
Cdd:PRK07514 313 pYDGERRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADgFFITGDLGKIDERGY 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGAtddknlvkPK--------AFVVLKRGYAPD-DQLKKDLK 475
Cdd:PRK07514 393 VHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGV--------PHpdfgegvtAVVVPKPGAALDeAAILAALK 464
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 476 EyvkkTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK07514 465 G----RLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
40-511 |
1.34e-52 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 185.98 E-value: 1.34e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI---- 115
Cdd:PRK13390 26 SYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVLVasaa 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELYSKFEPIKDKLRFlkhiivvnGQAPAGTISFDSFVAGASDQLEAAPItpddACFWLYSSGSTGQPKG-------- 187
Cdd:PRK13390 106 LDGLAAKVGADLPLRLSF--------GGEIDGFGSFEAALAGAGPRLTEQPC----GAVMLYSSGTTGFPKGiqpdlpgr 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIHLQHDMVYAAetyGKQVLGIQEHDICFSAAKLFFAYGLgNGLYFPFSVGATSVLMPvRPVPASVYETIARYRPTLYFG 267
Cdd:PRK13390 174 DVDAPGDPIVAI---ARAFYDISESDIYYSSAPIYHAAPL-RWCSMVHALGGTVVLAK-RFDAQATLGHVERYRITVTQM 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 268 VPTLYGSMLQEEGS------LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHI-FISNLPGKAKPGSSGKV 340
Cdd:PRK13390 249 VPTMFVRLLKLDADvrtrydVSSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMtFIDSPDWLAHPGSVGRS 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 341 VPGyDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD---WVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK13390 329 VLG-DLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPAhpfWTTVGDLGSVDEDGYLYLADRKSFMIIS 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PRK13390 408 GGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDEL 487
|
490
....*....|....
gi 700405679 498 PKTATGKLKRFELR 511
Cdd:PRK13390 488 PRTPTGKLVKGLLR 501
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
176-507 |
8.36e-52 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 179.00 E-value: 8.36e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 LYSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFpFSVGATSVLMPvRPVPASVYE 255
Cdd:cd17637 6 IHTAAVAGRPRGAV-LSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALAT-FHAGGANVVME-KFDPAEALE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGSMLQ--EEGSLDGVRLCVSAGEALPADIfKRWQQRHGVEIIDGIGSTELSHiFISNLPGKAK 333
Cdd:cd17637 83 LIEEEKVTLMGSFPPILSNLLDaaEKSGVDLSSLRHVLGLDAPETI-QRFEETTGATFWSLYGQTETSG-LVTLSPYRER 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 PGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRS-- 411
Cdd:cd17637 161 PGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTFRNGWHHTGDLGRFDEDGYLWYAGRKpe 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 412 NDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWI 491
Cdd:cd17637 241 KELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGATLT---ADELIEFVGSRIARYKKPRYV 317
|
330
....*....|....*.
gi 700405679 492 EFVDDLPKTATGKLKR 507
Cdd:cd17637 318 VFVEALPKTADGSIDR 333
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
37-510 |
1.29e-51 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 182.02 E-value: 1.29e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 37 NAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILII 116
Cdd:cd05907 4 QPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 DeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGSTGQPKGTIHLQHDMV 196
Cdd:cd05907 84 E--------------------------------------------------DPDDLATIIYTSGTTGRPKGVMLSHRNIL 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 197 YAAETYGKQV-LGIQEHDICFsaakLFFAYGLGN--GLYFPFSVGATSVLMpvrPVPASVYETIARYRPTLYFGVPTLY- 272
Cdd:cd05907 114 SNALALAERLpATEGDRHLSF----LPLAHVFERraGLYVPLLAGARIYFA---SSAETLLDDLSEVRPTVFLAVPRVWe 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 273 ----GSMLQEEGSLDG----------VRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIFISNLPGKAKPGSSG 338
Cdd:cd05907 187 kvyaAIKVKAVPGLKRklfdlavggrLRFAASGGAPLPAELLHFFRAL-GIPVYEGYGLTETSAVVTLNPPGDNRIGTVG 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 339 KVVPGYDARIVDEnfqdipvGEvgtLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:cd05907 266 KPLPGVEVRIADD-------GE---ILVRGPNVMLGYYKNPEATAEALDADgWLHTGDLGEIDEDGFLHITGRKKDLIIT 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 -GGIWVSPVEVEACLIEHPAVLECAVVGATDDKN--LVKPKAFVVLK------------RGYAPDDQLKKDLKEYVK--- 479
Cdd:cd05907 336 sGGKNISPEPIENALKASPLISQAVVIGDGRPFLvaLIVPDPEALEAwaeehgiaytdvAELAANPAVRAEIEAAVEaan 415
|
490 500 510
....*....|....*....|....*....|....*..
gi 700405679 480 KTLAHYKYPRWIEFVDDLP------KTATGKLKRFEL 510
Cdd:cd05907 416 ARLSRYEQIKKFLLLPEPFtiengeLTPTLKLKRPVI 452
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
29-511 |
1.99e-51 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 182.29 E-value: 1.99e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNAL-------KGLGIEMENRVALLLLdsefyplsFFGAIKIGAVPICLNTLMQPKD 101
Cdd:PRK07638 17 KIAIKENDRVLTYKDWFESVCKVANWLnekesknKTIAILLENRIEFLQL--------FAGAAMAGWTCVPLDIKWKQDE 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 102 YLYFLNDSRAKILIIDEELYSKFEPIKDK---LRFLKHIIVVNGQAPAGTISfdsfvagasdqLEAAPitpddacFWL-Y 177
Cdd:PRK07638 89 LKERLAISNADMIVTERYKLNDLPDEEGRvieIDEWKRMIEKYLPTYAPIEN-----------VQNAP-------FYMgF 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIHLQHDMVY----AAETYGkqvlgIQEHDICFSAAKLF---FAYGLGNGLYFpfsvGATSVLMPvRPVP 250
Cdd:PRK07638 151 TSGSTGKPKAFLRAQQSWLHsfdcNVHDFH-----MKREDSVLIAGTLVhslFLYGAISTLYV----GQTVHLMR-KFIP 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 251 ASVYETIARYRPTLYFGVPTLYGSMLQEEGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELSHI-FISNL 328
Cdd:PRK07638 221 NQVLDKLETENISVMYTVPTMLESLYKENRVIENKMKIISSGAKWEAEAKEKIKNIFpYAKLYEFYGASELSFVtALVDE 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 329 PGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYI 408
Cdd:PRK07638 301 ESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYIIGGVLARELNADGWMTVRDVGYEDEEGFIYIV 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 409 GRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVvlkRGYAPddqlKKDLKEYVKKTLAHYKYP 488
Cdd:PRK07638 381 GREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII---KGSAT----KQQLKSFCLQRLSSFKIP 453
|
490 500
....*....|....*....|...
gi 700405679 489 RWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK07638 454 KEWHFVDEIPYTNSGKIARMEAK 476
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
4-512 |
4.12e-51 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 184.06 E-value: 4.12e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 4 ELNLpeTFNAadyfVDRNIREGRGQKTAILC------RDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALllldseFYP 77
Cdd:cd05967 48 RLNT--CYNA----LDRHVEAGRGDQIALIYdspvtgTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVII------YMP 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 78 L------SFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI-----IDEELYSKFEPIKDKLRFL-----KHIIVVN 141
Cdd:cd05967 116 MipeaaiAMLACARIGAIHSVVFGGFAAKELASRIDDAKPKLIVtascgIEPGKVVPYKPLLDKALELsghkpHHVLVLN 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 142 -GQAPAGT------ISFDSFVAGASdQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDI 214
Cdd:cd05967 196 rPQVPADLtkpgrdLDWSELLAKAE-PVDCVPVAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDV 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 215 CFSAAKLFFAYGLGNGLYFPFSVGATSVL---MPVR-PVPASVYETIARYRPTLYFGVPTLYGSMLQEEGSLDGVRLC-- 288
Cdd:cd05967 275 WWAASDVGWVVGHSYIVYGPLLHGATTVLyegKPVGtPDPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPDGKYIKKYdl 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 289 ------VSAGEALPADIFKrWQQRH-GVEIIDGIGSTELSHIFISNLPGKA----KPGSSGKVVPGYDARIVDENFQDIP 357
Cdd:cd05967 355 sslrtlFLAGERLDPPTLE-WAENTlGVPVIDHWWQTETGWPITANPVGLEplpiKAGSPGKPVPGYQVQVLDEDGEPVG 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 358 VGEVGTLLIKGD---SAAAAYWNKHQKTKSTMLGD---WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACL 431
Cdd:cd05967 434 PNELGNIVIKLPlppGCLLTLWKNDERFKKLYLSKfpgYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESV 513
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 432 IEHPAVLECAVVGATDD-KNLVkPKAFVVLKRGYAPD-DQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFE 509
Cdd:cd05967 514 LSHPAVAECAVVGVRDElKGQV-PLGLVVLKEGVKITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRT 592
|
...
gi 700405679 510 LRA 512
Cdd:cd05967 593 LRK 595
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
27-512 |
4.52e-51 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 181.34 E-value: 4.52e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLSFFGAIKIGAVPICLNTLMQPKDYLYF 105
Cdd:cd12118 18 PDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTpAMY-ELHFGVPMAGAVLNALNTRLDAEEIAFI 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEElyskFEpikdklrflkhiivvngqapagtisFDSFVAGASDqlEAAPITPDDACFWL---YSSGST 182
Cdd:cd12118 97 LRHSEAKVLFVDRE----FE-------------------------YEDLLAEGDP--DFEWIPPADEWDPIalnYTSGTT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 183 GQPKGTIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAyglgNGLYFPFSV---GATSVLMpvRPV-PASVYETIA 258
Cdd:cd12118 146 GRPKGVVY-HHRGAYLNALANILEWEMKQHPVYLWTLPMFHC----NGWCFPWTVaavGGTNVCL--RKVdAKAIYDLIE 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 259 RYRPTLYFGVPTLYgSML-----QEEGSLDGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIFIS------- 326
Cdd:cd12118 219 KHKVTHFCGAPTVL-NMLanappSDARPLPHRVHVMTAGAPPPAAVLAKMEEL-GFDVTHVYGLTETYGPATVcawkpew 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 -NLPG------KAKPGSSgkvVPGYDA-RIVD-ENFQDIP-----VGEVgtlLIKGDSAAAAYWNKHQKTKSTMLGDWVN 392
Cdd:cd12118 297 dELPTeerarlKARQGVR---YVGLEEvDVLDpETMKPVPrdgktIGEI---VFRGNIVMKGYLKNPEATAEAFRGGWFH 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 393 TDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkk 472
Cdd:cd12118 371 SGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE--- 447
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 700405679 473 DLKEYVKKTLAHYKYPRWIEFvDDLPKTATGKLKRFELRA 512
Cdd:cd12118 448 EIIAFCREHLAGFMVPKTVVF-GELPKTSTGKIQKFVLRD 486
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
17-511 |
2.92e-50 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 180.78 E-value: 2.92e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 17 FVDRNIRegrgqktailcrdnaFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTL 96
Cdd:PRK08315 37 YRDQGLR---------------WTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINPA 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 97 MQPKDYLYFLNDSRAKILIIDE----------------ELYSKfEPIK---DKLRFLKHIIVVNGQAPAGTISFDSFVAG 157
Cdd:PRK08315 102 YRLSELEYALNQSGCKALIAADgfkdsdyvamlyelapELATC-EPGQlqsARLPELRRVIFLGDEKHPGMLNFDELLAL 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 158 AS----DQLEA--APITPDDACFWLYSSGSTGQPKGTIHLQHDMV----YAAETygkqvLGIQEHD-ICFSAAkLFFAYG 226
Cdd:PRK08315 181 GRavddAELAArqATLDPDDPINIQYTSGTTGFPKGATLTHRNILnngyFIGEA-----MKLTEEDrLCIPVP-LYHCFG 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 227 --LGN-GLyfpFSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADI 299
Cdd:PRK08315 255 mvLGNlAC---VTHGATMVYPGEGFDPLATLAAVEEERCTALYGVPTMFIAELDhpdfARFDLSSLRTGIMAGSPCPIEV 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 300 FKRWQQR-HGVEIIDGIGSTELSH-IFISNL--PGKAKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAA 374
Cdd:PRK08315 332 MKRVIDKmHMSEVTIAYGMTETSPvSTQTRTddPLEKRVTTVGRALPHLEVKIVDpETGETVPRGEQGELCTRGYSVMKG 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 375 YWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDK---N 450
Cdd:PRK08315 412 YWNDPEKTAEAIDADgWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKygeE 491
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 451 LVkpkAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK08315 492 VC---AWIILRPGATLTEE---DVRDFCRGKIAHYKIPRYIRFVDEFPMTVTGKIQKFKMR 546
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
28-512 |
3.04e-50 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 179.31 E-value: 3.04e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK06145 17 DRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYILG 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELyskfepikDKLRFLKHIIVVNGQAPAGTISfdSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK06145 97 DAGAKLLLVDEEF--------DAIVALETPKIVIDAAAQADSR--RLAQGGLEIPPQAAVAPTDLVRLMYTSGTTDRPKG 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMpvRPV-PASVYETIARYRPTLYF 266
Cdd:PRK06145 167 VMH-SYGNLHWKSIDHVIALGLTASERLLVVGPLYHVGAFDLPGIAVLWVGGTLRIH--REFdPEAVLAAIERHRLTCAW 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 267 GVPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTEL--SHIFISNLPGKAKPGSSGK 339
Cdd:PRK06145 244 MAPVMLSRVLtvpdRDRFDLDSLAWCIGGGEKTPESRIRDFTRVfTRARYIDAYGLTETcsGDTLMEAGREIEKIGSTGR 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 340 VVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGG 419
Cdd:PRK06145 324 ALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGDWFRSGDVGYLDEEGFLYLTDRKKDMIISGG 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 420 IWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYApddqLKKD-LKEYVKKTLAHYKYPRWIEFVDDLP 498
Cdd:PRK06145 404 ENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGAT----LTLEaLDRHCRQRLASFKVPRQLKVRDELP 479
|
490
....*....|....
gi 700405679 499 KTATGKLKRFELRA 512
Cdd:PRK06145 480 RNPSGKVLKRVLRD 493
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
26-512 |
4.66e-50 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 179.41 E-value: 4.66e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSefyPLSFfgaIKIGAVPIC------LNTLMQP 99
Cdd:PRK06188 25 YPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNR---PEVL---MAIGAAQLAglrrtaLHPLGSL 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 100 KDYLYFLNDSRAKILIIDEELY-SKFEPIKDKLRFLKHIIVVnGQAPAGTisfdSFVAGAsDQLEAAPITPDDA---CFW 175
Cdd:PRK06188 99 DDHAYVLEDAGISTLIVDPAPFvERALALLARVPSLKHVLTL-GPVPDGV----DLLAAA-AKFGPAPLVAAALppdIAG 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 L-YSSGSTGQPKGTIHLQHDMVYAAETygkqVLGIQE--HDICF-------SAAKLFFAYGLGNGlyfpfsvgATSVLMP 245
Cdd:PRK06188 173 LaYTGGTTGKPKGVMGTHRSIATMAQI----QLAEWEwpADPRFlmctplsHAGGAFFLPTLLRG--------GTVIVLA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 vRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEG-------SLDGVRLCVSAgeALPADIfkrwqqRHGVEIIDGI--- 315
Cdd:PRK06188 241 -KFDPAEVLRAIEEQRITATFLVPTMIYALLDHPDlrtrdlsSLETVYYGASP--MSPVRL------AEAIERFGPIfaq 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 316 --GSTElSHIFISNLPGK----AKP---GSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTM 386
Cdd:PRK06188 312 yyGQTE-APMVITYLRKRdhdpDDPkrlTSCGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAF 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 LGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAP 466
Cdd:PRK06188 391 RDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAV 470
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 700405679 467 DDQlkkDLKEYVK--KTLAHykYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK06188 471 DAA---ELQAHVKerKGSVH--APKQVDFVDSLPLTALGKPDKKALRA 513
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
26-507 |
5.74e-50 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 178.16 E-value: 5.74e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL 103
Cdd:cd12117 10 TPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAayVPLDPE---LPAERL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 -YFLNDSRAKILIIDEELyskfepiKDKLRFLKHIIVVNGQAPAGtisfdsfvagaSDQLEAAPITPDDACFWLYSSGST 182
Cdd:cd12117 87 aFMLADAGAKVLLTDRSL-------AGRAGGLEVAVVIDEALDAG-----------PAGNPAVPVSPDDLAYVMYTSGST 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 183 GQPKGTI--H-----LQHDMVYAAETYGKQVLgiQEHDICFSAAKLffayglgnGLYFPFSVGATSVLMPVRPV--PASV 253
Cdd:cd12117 149 GRPKGVAvtHrgvvrLVKNTNYVTLGPDDRVL--QTSPLAFDASTF--------EIWGALLNGARLVLAPKGTLldPDAL 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 YETIARYRPTLYFGVPTLYGSMLQEE-GSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTE-----LSHiFIS 326
Cdd:cd12117 219 GALIAEEGVTVLWLTAALFNQLADEDpECFAGLRELLTGGEVVSPPHVRRVLAACpGLRLVNGYGPTEnttftTSH-VVT 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTK-----------STML--GDWVNt 393
Cdd:cd12117 298 ELDEVAGSIPIGRPIANTRVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAerfvadpfgpgERLYrtGDLAR- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 394 ddkyaVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATDDKNLVkpkAFVVLKRGYAPDdql 470
Cdd:cd12117 377 -----WLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVvreDAGGDKRLV---AYVVAEGALDAA--- 445
|
490 500 510
....*....|....*....|....*....|....*..
gi 700405679 471 kkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd12117 446 --ELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDR 480
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
26-507 |
8.25e-50 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 177.85 E-value: 8.25e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTlMQPKDYL-Y 104
Cdd:cd17646 11 TPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDP-GYPADRLaY 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEELYSKFEPikdklrflkhiivvngqAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQ 184
Cdd:cd17646 90 MLADAGPAVVLTTADLAARLPA-----------------GGDVALLGDEALAAPPATPPLVPPRPDNLAYVIYTSGSTGR 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGTIHLQHDMVyaaetygKQVLGIQEHdicfsaaklffaYGLGNG-----------------LYFPFSVGATSVLmpVR 247
Cdd:cd17646 153 PKGVMVTHAGIV-------NRLLWMQDE------------YPLGPGdrvlqktplsfdvsvweLFWPLVAGARLVV--AR 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 248 P----VPASVYETIARYRPT-LYFgVPTLYGSMLQE--EGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTEL 320
Cdd:cd17646 212 PgghrDPAYLAALIREHGVTtCHF-VPSMLRVFLAEpaAGSCASLRRVFCSGEALPPELAARFLALPGAELHNLYGPTEA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 321 ShIFISNLP--GKAKPGSS--GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVN---- 392
Cdd:cd17646 291 A-IDVTHWPvrGPAETPSVpiGRPVPNTRLYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPFGpgsr 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 393 ---TDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATDDKNLVkpkAFVVLKRGYAP 466
Cdd:cd17646 370 myrTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVaraAPAGAARLV---GYVVPAAGAAG 446
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 700405679 467 DDQlkKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17646 447 PDT--AALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDR 485
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
3-504 |
5.21e-49 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 176.61 E-value: 5.21e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 3 VELNLPETFNA-ADYFVDRniregrgqkTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFF 81
Cdd:PRK07798 1 MAWNIADLFEAvADAVPDR---------VALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAML 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 82 GAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVN----GQAPAGTISFDSFVAG 157
Cdd:PRK07798 72 GAFKARAVPVNVNYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLPRLPKLRTLVVVEdgsgNDLLPGAVDYEDALAA 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 158 ASDQLEAAPITPDDAcFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQ---EHDICFSAA-----KLFFAYGL-- 227
Cdd:PRK07798 152 GSPERDFGERSPDDL-YLLYTGGTTGMPKGVMWRQEDIFRVLLGGRDFATGEPiedEEELAKRAAagpgmRRFPAPPLmh 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 228 GNGLYFPFS---VGATSVLMPV-RPVPASVYETIARYRPTLYFGVPTLYGS-MLQE-----EGSLDGVRLCVSAGEALPA 297
Cdd:PRK07798 231 GAGQWAAFAalfSGQTVVLLPDvRFDADEVWRTIEREKVNVITIVGDAMARpLLDAleargPYDLSSLFAIASGGALFSP 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 298 DIFKRWQ-QRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGE-VGTLLIKGDSAAAAY 375
Cdd:PRK07798 311 SVKEALLeLLPNVVLTDSIGSSETGFGGSGTVAKGAVHTGGPRFTIGPRTVVLDEDGNPVEPGSgEIGWIARRGHIPLGY 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 376 WNKHQKTKST----------MLGDWVNtddkyaVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGA 445
Cdd:PRK07798 391 YKDPEKTAETfptidgvryaIPGDRAR------VEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGV 464
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 446 TDDK--NLVkpKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:PRK07798 465 PDERwgQEV--VAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
39-511 |
6.92e-49 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 176.44 E-value: 6.92e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:PRK07008 40 YTYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFEPIKDKLRFLKHIIVVNGQA--PAGTI---SFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQH 193
Cdd:PRK07008 120 TFLPLVDALAPQCPNVKGWVAMTDAAhlPAGSTpllCYETLVGAQDGDYDWPRFDENQASSLCYTSGTTGNPKGALYSHR 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMV---YAAETygKQVLGIQEHDICFSAAKLFF--AYGLgnglyfPFS---VGATSVLmpvrPVPA----SVYETIARYR 261
Cdd:PRK07008 200 STVlhaYGAAL--PDAMGLSARDAVLPVVPMFHvnAWGL------PYSaplTGAKLVL----PGPDldgkSLYELIEAER 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 262 PTLYFGVPTLYGSML---QEEG-SLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELS-----------HifiS 326
Cdd:PRK07008 268 VTFSAGVPTVWLGLLnhmREAGlRFSTLRRTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEMSplgtlcklkwkH---S 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NLPGKAKPG---SSGKVVPGYDARIVDENFQDIPVGEV--GTLLIKGDSAAAAYWnkhQKTKSTMLGDWVNTDDKYAVDE 401
Cdd:PRK07008 345 QLPLDEQRKlleKQGRVIYGVDMKIVGDDGRELPWDGKafGDLQVRGPWVIDRYF---RGDASPLVDGWFPTGDVATIDA 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 402 EGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKrgyaPDDQLKKD--LKEYVK 479
Cdd:PRK07008 422 DGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKR----PGAEVTREelLAFYEG 497
|
490 500 510
....*....|....*....|....*....|..
gi 700405679 480 KTlAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK07008 498 KV-AKWWIPDDVVFVDAIPHTATGKLQKLKLR 528
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
29-507 |
3.34e-48 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 173.25 E-value: 3.34e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-YF 105
Cdd:cd12116 3 ATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAayVPLDPD---YPADRLrYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEELyskfepiKDKLRflkhiivvngqAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQP 185
Cdd:cd12116 80 LEDAEPALVLTDDAL-------PDRLP-----------AGLPVLLLALAAAAAAPAAPRTPVSPDDLAYVIYTSGSTGRP 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGT-------IHLQHDMvyaaetygKQVLGIQEHD-------ICFSAAKLffayglgnGLYFPFSVGATSVLMP--VRPV 249
Cdd:cd12116 142 KGVvvshrnlVNFLHSM--------RERLGLGPGDrllavttYAFDISLL--------ELLLPLLAGARVVIAPreTQRD 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 250 PASVYETIARYRPTLYFGVPTLYGSMLQ-EEGSLDGVR-LCvsAGEALPADIFKRWQQRHGvEIIDGIGSTELShIF--I 325
Cdd:cd12116 206 PEALARLIEAHSITVMQATPATWRMLLDaGWQGRAGLTaLC--GGEALPPDLAARLLSRVG-SLWNLYGPTETT-IWstA 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 326 SNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTDDKY 397
Cdd:cd12116 282 ARVTAAAGPIPIGRPLANTQVYVLDAALRPVPPGVPGELYIGGDGVAQGYLGRPALTAERFVPDpfagpgsrLYRTGDLV 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 398 AVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV--GATDDKNLVkpkAFVVLKRGYAPDdqlKKDLK 475
Cdd:cd12116 362 RRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVvrEDGGDRRLV---AYVVLKAGAAPD---AAALR 435
|
490 500 510
....*....|....*....|....*....|...
gi 700405679 476 EYVKKTLAHYKYP-RWIEfVDDLPKTATGKLKR 507
Cdd:cd12116 436 AHLRATLPAYMVPsAFVR-LDALPLTANGKLDR 467
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
28-511 |
3.47e-48 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 172.55 E-value: 3.47e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:cd17649 2 DAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYMLE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILiideelyskfepikdklrfLKHiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGSTGQPKG 187
Cdd:cd17649 82 DSGAGLL-------------------LTH-------------------------------HPRQLAYVIYTSGSTGTPKG 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TI--------HLQHDMVYAAETYGKQVLgiQEHDICFSAAKlffayglgNGLYFPFSVGATSVLMPVRPV--PASVYETI 257
Cdd:cd17649 112 VAvshgplaaHCQATAERYGLTPGDREL--QFASFNFDGAH--------EQLLPPLICGACVVLRPDELWasADELAEMV 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLyFGVPTLYGSMLQEEGSLDG------VRLCVSAGEALPADIFKRWQQRHgVEIIDGIGSTELShifISNLPGK 331
Cdd:cd17649 182 RELGVTV-LDLPPAYLQQLAEEADRTGdgrppsLRLYIFGGEALSPELLRRWLKAP-VRLFNAYGPTEAT---VTPLVWK 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 332 AKPGSS--------GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTDD 395
Cdd:cd17649 257 CEAGAAragasmpiGRPLGGRSAYILDADLNPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDpfgapgsrLYRTGD 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 396 --KYAVDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD--KNLVkpkAFVVLKrgyAPDDQ-- 469
Cdd:cd17649 337 laRWRDD--GVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAggKQLV---AYVVLR---AAAAQpe 408
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 700405679 470 LKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd17649 409 LRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
9-512 |
5.51e-48 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 173.79 E-value: 5.51e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 9 ETFNAadyFVDRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYpLSFFGAIKIG 87
Cdd:COG1021 25 ETLGD---LLRRRAER-HPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVaEFV-IVFFALFRAG 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 88 AVPIclNTLMQPKDY--LYFLNDSRAKILIIDEElYSKF------EPIKDKLRFLKHIIVVNgqAPAGTISFDSFVAGAS 159
Cdd:COG1021 100 AIPV--FALPAHRRAeiSHFAEQSEAVAYIIPDR-HRGFdyralaRELQAEVPSLRHVLVVG--DAGEFTSLDALLAAPA 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 160 DQLEAAPiTPDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAYGLGN-GLYFPFSVG 238
Cdd:COG1021 175 DLSEPRP-DPDDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRASA-EICGLDADTVYLAALPAAHNFPLSSpGVLGVLYAG 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 239 ATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRwqqrhgveIIDG 314
Cdd:COG1021 253 GTVVLAP-DPSPDTAFPLIERERVTVTALVPPLALLWLDaaerSRYDLSSLRVLQVGGAKLSPELARR--------VRPA 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 315 IGSTeLSHIFisnlpGKAK----------P-----GSSGKVVPGYD-ARIVDENFQDIPVGEVGTLLIKGDSAAAAYWN- 377
Cdd:COG1021 324 LGCT-LQQVF-----GMAEglvnytrlddPeevilTTQGRPISPDDeVRIVDEDGNPVPPGEVGELLTRGPYTIRGYYRa 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 378 -KHQKTKSTMLGdWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDknLV--KP 454
Cdd:COG1021 398 pEHNARAFTPDG-FYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDE--YLgeRS 474
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 700405679 455 KAFVVLkRGYAPD-DQLKKDLKEyvkKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:COG1021 475 CAFVVP-RGEPLTlAELRRFLRE---RGLAAFKLPDRLEFVDALPLTAVGKIDKKALRA 529
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
39-512 |
6.98e-48 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 173.63 E-value: 6.98e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKiLIIDE 118
Cdd:PLN02246 51 YTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAK-LIITQ 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSkfepikDKLRFLKH----IIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQH- 193
Cdd:PLN02246 130 SCYV------DKLKGLAEddgvTVVTIDDPPEGCLHFSELTQADENELPEVEISPDDVVALPYSSGTTGLPKGVM-LTHk 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMVyaaETYGKQV------LGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPAsVYETIARYRPTLYFG 267
Cdd:PLN02246 203 GLV---TSVAQQVdgenpnLYFHSDDVILCVLPMFHIYSLNSVLLCGLRVGAAILIMPKFEIGA-LLELIQRHKVTIAPF 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 268 VPTLYGSMLQ----EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEII-DGIGSTELSHIFISNL-----PGKAKPGSS 337
Cdd:PLN02246 279 VPPIVLAIAKspvvEKYDLSSIRMVLSGAAPLGKELEDAFRAKLPNAVLgQGYGMTEAGPVLAMCLafakePFPVKSGSC 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDML 415
Cdd:PLN02246 359 GTVVRNAELKIVDpETGASLPRNQPGEICIRGPQIMKGYLNDPEATANTIDKDgWLHTGDIGYIDDDDELFIVDRLKELI 438
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 416 KVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVlkrgYAPDDQLKKD-LKEYVKKTLAHYKYPRWIEFV 494
Cdd:PLN02246 439 KYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVV----RSNGSEITEDeIKQFVAKQVVFYKRIHKVFFV 514
|
490
....*....|....*...
gi 700405679 495 DDLPKTATGKLKRFELRA 512
Cdd:PLN02246 515 DSIPKAPSGKILRKDLRA 532
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
31-511 |
7.63e-48 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 172.96 E-value: 7.63e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLL-DSEFYPLSFfGAIKIGAVPICLNTLMQPKDYLYFLNDS 109
Cdd:PRK12406 4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRnDFAFFEAAY-AAMRLGAYAVPVNWHFKPEEIAYILEDS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 110 RAKILIIDEELyskFEPIKDKLRflKHIIVV-----------------NGQAPAGTISFDSFVAGASDQleAAPITPDDA 172
Cdd:PRK12406 83 GARVLIAHADL---LHGLASALP--AGVTVLsvptppeiaaayrispaLLTPPAGAIDWEGWLAQQEPY--DGPPVPQPQ 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 173 CFwLYSSGSTGQPKGTI-------HLQHDMVYAAETYGkQVLGIQE-------HdicfSAAKlffAYGLgnglyFPFSVG 238
Cdd:PRK12406 156 SM-IYTSGTTGHPKGVRraaptpeQAAAAEQMRALIYG-LKPGIRAlltgplyH----SAPN---AYGL-----RAGRLG 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 239 ATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGSMLQ------EEGSLDGVRLCVSAGEALPADIfKR-----Wqqrh 307
Cdd:PRK12406 222 GVLVLQP-RFDPEELLQLIERHRITHMHMVPTMFIRLLKlpeevrAKYDVSSLRHVIHAAAPCPADV-KRamiewW---- 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 308 GVEIIDGIGSTELSHI-FISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAA-AYWNKHQKTKST 385
Cdd:PRK12406 296 GPVIYEYYGSTESGAVtFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRAEI 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 386 MLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDK------NLVKPKAFVV 459
Cdd:PRK12406 376 DRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEfgealmAVVEPQPGAT 455
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 700405679 460 LKRGyapddqlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PRK12406 456 LDEA---------DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
28-507 |
9.58e-48 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 178.13 E-value: 9.58e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-Y 104
Cdd:COG1020 491 DAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAayVPLDPA---YPAERLaY 567
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEELYSKFepikdklrflkhiivvnGQAPAGTISFDSF-VAGASDQLEAAPITPDDACFWLYSSGSTG 183
Cdd:COG1020 568 MLEDAGARLVLTQSALAARL-----------------PELGVPVLALDALaLAAEPATNPPVPVTPDDLAYVIYTSGSTG 630
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 184 QPKGT-------IHLQHDMVyaaetygkQVLGIQEHD-------ICFSAAKL-FFAyglgnglyfPFSVGATSVLMP--V 246
Cdd:COG1020 631 RPKGVmvehralVNLLAWMQ--------RRYGLGPGDrvlqfasLSFDASVWeIFG---------ALLSGATLVLAPpeA 693
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 247 RPVPASVYETIARYRPTLYFGVPTLYGSMLQEE-GSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTElSHIF 324
Cdd:COG1020 694 RRDPAALAELLARHRVTVLNLTPSLLRALLDAApEALPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTE-TTVD 772
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 --ISNLPGKAKPGSS---GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKS--------------- 384
Cdd:COG1020 773 stYYEVTPPDADGGSvpiGRPIANTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAErfvadpfgfpgarly 852
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 385 -TmlGDWV--NTDdkyavdeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATD---DKNLVkpkAFV 458
Cdd:COG1020 853 rT--GDLArwLPD--------GNLEFLGRADDQVKIRGFRIELGEIEAALLQHPGVREAVVVAREDapgDKRLV---AYV 919
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 700405679 459 VLKRGYAPDDQLkkdLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:COG1020 920 VPEAGAAAAAAL---LRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDR 965
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
37-512 |
2.91e-46 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 168.28 E-value: 2.91e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 37 NAFTYAQVQAGMNKVGNALKGLGIEMENrVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILII 116
Cdd:cd05909 6 TSLTYRKLLTGAIALARKLAKMTKEGEN-VGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 DEELYSK-----------------FEPIKDKLRFLKHIIVvngqAPAGTISFDSFVAgasdQLEAAPITPDDACFWLYSS 179
Cdd:cd05909 85 SKQFIEKlklhhlfdveydarivyLEDLRAKISKADKCKA----FLAGKFPPKWLLR----IFGVAPVQPDDPAVILFTS 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 180 GSTGQPKGTIHLQHDMVYAAEtygkQVLGI---QEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASVYET 256
Cdd:cd05909 157 GSEGLPKGVVLSHKNLLANVE----QITAIfdpNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFHPNPLDYKKIPEL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVPTLYGSMLQ--EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKA-K 333
Cdd:cd05909 233 IYDKKATILLGTPTFLRGYARaaHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGYGTTECSPVISVNTPQSPnK 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 PGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSN 412
Cdd:cd05909 313 EGTVGRPLPGMEVKIVSvETHEEVPIGEGGLLLVRGPNVMLGYLNEPELTSFAFGDGWYDTGDIGKIDGEGFLTITGRLS 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 413 DMLKVGGIWVSPVEVEACLIEH-PAVLECAVVGATDDKnlvKPKAFVVLkrgYAPDDQLKKDLKEYVKKT-LAHYKYPRW 490
Cdd:cd05909 393 RFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGR---KGEKIVLL---TTTTDTDPSSLNDILKNAgISNLAKPSY 466
|
490 500
....*....|....*....|..
gi 700405679 491 IEFVDDLPKTATGKLKRFELRA 512
Cdd:cd05909 467 IHQVEEIPLLGTGKPDYVTLKA 488
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
168-512 |
3.04e-46 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 167.86 E-value: 3.04e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 168 TPDDACFWLYSSGSTGQPKGT------IHLQHDMVYAA-ETYGKQVLGiqeHdicfsAAKLFFAYGLGNGLYFPFSVGAT 240
Cdd:PRK07787 126 DPDAPALIVYTSGTTGPPKGVvlsrraIAADLDALAEAwQWTADDVLV---H-----GLPLFHVHGLVLGVLGPLRIGNR 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 241 SVLMpVRPVPASVYETIARyRPTLYFGVPTLYGSMLQEEGS---LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGS 317
Cdd:PRK07787 198 FVHT-GRPTPEAYAQALSE-GGTLYFGVPTVWSRIAADPEAaraLRGARLLVSGSAALPVPVFDRLAALTGHRPVERYGM 275
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 318 TElSHIFISNLP-GKAKPGSSGKVVPGYDARIVDENFQDIPV-GE-VGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNT 393
Cdd:PRK07787 276 TE-TLITLSTRAdGERRPGWVGLPLAGVETRLVDEDGGPVPHdGEtVGELQVRGPTLFDGYLNRPDATAAAFTADgWFRT 354
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 394 DDKYAVDEEGYFYYIGR-SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD---KNLVkpkAFVVLKRGYAPDdq 469
Cdd:PRK07787 355 GDVAVVDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDdlgQRIV---AYVVGADDVAAD-- 429
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 700405679 470 lkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK07787 430 ---ELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLS 469
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
134-512 |
4.39e-46 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 167.55 E-value: 4.39e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 134 LKHIIVVNGQAPAGTIS--FDSFVAGASDQLEaaPITPDDACFW--LYSSGSTGQPKGTI-HLQHDMVYAAETYGKQVL- 207
Cdd:cd05929 87 IKAAALVCGLFTGGGALdgLEDYEAAEGGSPE--TPIEDEAAGWkmLYSGGTTGRPKGIKrGLPGGPPDNDTLMAAALGf 164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 208 GIQEHDICFSAAKLFFAYGLG---NGLYfpfsVGATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEGS--- 281
Cdd:cd05929 165 GPGADSVYLSPAPLYHAAPFRwsmTALF----MGGTLVLME-KFDPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAvrn 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 282 ---LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHI-FISNLPGKAKPGSSGKVVPGyDARIVDENFQDIP 357
Cdd:cd05929 240 aydLSSLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYYGGTEGQGLtIINGEEWLTHPGSVGRAVLG-KVHILDEDGNEVP 318
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 358 VGEVGTLLIKGdSAAAAYWNKHQKTK-STMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPA 436
Cdd:cd05929 319 PGEIGEVYFAN-GPGFEYTNDPEKTAaARNEGGWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPK 397
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 437 VLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:cd05929 398 VLDAAVVGVPDEELGQRVHAVVQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
176-507 |
8.91e-46 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 163.06 E-value: 8.91e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 LYSSGSTGQPKGTI--HLQHDMVYAAETygkQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASV 253
Cdd:cd17638 6 MFTSGTTGRSKGVMcaHRQTLRAAAAWA---DCADLTEDDRYLIINPFFHTFGYKAGIVACLLTGATVVPVAVFDVDAIL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 yETIARYRPTLYFGVPTLYGSMLQE----EGSLDGVRLCVSAGEALPADIFKRWQQRHGVEII-DGIGSTElshifiSNL 328
Cdd:cd17638 83 -EAIERERITVLPGPPTLFQSLLDHpgrkKFDLSSLRAAVTGAATVPVELVRRMRSELGFETVlTAYGLTE------AGV 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 329 PGKAKPG--------SSGKVVPGYDARIVDEnfqdipvGEVgtlLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAV 399
Cdd:cd17638 156 ATMCRPGddaetvatTCGRACPGFEVRIADD-------GEV---LVRGYNVMQGYLDDPEATAEAIDADgWLHTGDVGEL 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 400 DEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVK 479
Cdd:cd17638 226 DERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVTLTEE---DVIAWCR 302
|
330 340
....*....|....*....|....*...
gi 700405679 480 KTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17638 303 ERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
169-512 |
3.98e-45 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 162.26 E-value: 3.98e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETyGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLmpvrP 248
Cdd:cd05944 1 SDDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWM-LALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVL----A 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPA---------SVYETIARYRPTLYFGVPTLYGSMLQEEGSLD--GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGS 317
Cdd:cd05944 76 GPAgyrnpglfdNFWKLVERYRITSLSTVPTVYAALLQVPVNADisSLRFAMSGAAPLPVELRARFEDATGLPVVEGYGL 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 318 TELSHIFISNLPGKA-KPGSSGKVVPGYDARIVDEN-----FQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWV 391
Cdd:cd05944 156 TEATCLVAVNPPDGPkRPGSVGLRLPYARVRIKVLDgvgrlLRDCAPDEVGEICVAGPGVFGGYLYTEGNKNAFVADGWL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 392 NTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRG-YAPDDQL 470
Cdd:cd05944 236 NTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGaVVEEEEL 315
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 700405679 471 KKDLKEYVKKTLAhykYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:cd05944 316 LAWARDHVPERAA---VPKHIEVLEELPVTAVGKVFKPALRA 354
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
28-507 |
9.17e-45 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 163.25 E-value: 9.17e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYLYF 105
Cdd:cd17643 2 EAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGayVPIDPA---YPVERIAF 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 -LNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQ 184
Cdd:cd17643 79 iLADSGPSLLLTD---------------------------------------------------PDDLAYVIYTSGSTGR 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGTIhLQHDMVYAAETYGKQVLGIQEHDICFsaakLFFAYGlgnglyFPFSV---------GATSVLMP--VRPVPASV 253
Cdd:cd17643 108 PKGVV-VSHANVLALFAATQRWFGFNEDDVWT----LFHSYA------FDFSVweiwgallhGGRLVVVPyeVARSPEDF 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 YETIARYRPTLYFGVPTLYGSMLQEE----GSLDGVRLCVSAGEALPADIFKRWQQRHGV---EIIDGIGSTE----LSH 322
Cdd:cd17643 177 ARLLRDEGVTVLNQTPSAFYQLVEAAdrdgRDPLALRYVIFGGEALEAAMLRPWAGRFGLdrpQLVNMYGITEttvhVTF 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 323 IFISNLPGKAKPGSS-GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML-------------- 387
Cdd:cd17643 257 RPLDAADLPAAAASPiGRPLPGLRVYVLDADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVanpfggpgsrmyrt 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 388 GDWVntddKYAVDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATDDKNLVkpkAFVVLKRGY 464
Cdd:cd17643 337 GDLA----RRLPD--GELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIvreDEPGDTRLV---AYVVADDGA 407
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 700405679 465 APDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17643 408 AAD---IAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDR 447
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
39-511 |
1.04e-44 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 165.01 E-value: 1.04e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSK 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFEPIKDKLRFLKHIIVVNGQAP-AGTISFDSFVAGASD-QLEAAPITP------DDACFWLYSSGSTGQPKGtIH 190
Cdd:cd17642 125 KGLQKVLNVQKKLKIIKTIIILDSKEDyKGYQCLYTFITQNLPpGFNEYDFKPpsfdrdEQVALIMNSSGSTGLPKG-VQ 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 LQHDMVYAA------ETYGKQvlgIQEHDICFSAAKLFFAYGLGNGLYFpFSVGATSVLMPvRPVPASVYETIARYRPTL 264
Cdd:cd17642 204 LTHKNIVARfshardPIFGNQ---IIPDTAILTVIPFHHGFGMFTTLGY-LICGFRVVLMY-KFEEELFLRSLQDYKVQS 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 265 YFGVPTLYG----SMLQEEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEII-DGIGSTE-LSHIFISNlPGKAKPGSSG 338
Cdd:cd17642 279 ALLVPTLFAffakSTLVDKYDLSNLHEIASGGAPLSKEVGEAVAKRFKLPGIrQGYGLTEtTSAILITP-EGDDKPGAVG 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 339 KVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLK 416
Cdd:cd17642 358 KVVPFFYAKVVDlDTGKTLGPNERGELCVKGPMIMKGYVNNPEATKALIDKDgWLHSGDIAYYDEDGHFFIVDRLKSLIK 437
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 417 VGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPR-WIEFVD 495
Cdd:cd17642 438 YKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGKTMTEK---EVMDYVASQVSTAKRLRgGVKFVD 514
|
490
....*....|....*.
gi 700405679 496 DLPKTATGKLKRFELR 511
Cdd:cd17642 515 EVPKGLTGKIDRRKIR 530
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
173-512 |
1.21e-44 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 159.80 E-value: 1.21e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 173 CFWLYSSGSTGQPKGTIHLQHDMVYAAETyGKQVLGIQEHDICFSAAKLFFAYGLGNgLYFPFSVGATSVLMPVRPvpaS 252
Cdd:cd17630 3 ATVILTSGSTGTPKAVVHTAANLLASAAG-LHSRLGFGGGDSWLLSLPLYHVGGLAI-LVRSLLAGAELVLLERNQ---A 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARYRPTLYFGVPTLYGSML---QEEGSLDGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTEL-SHIFISNL 328
Cdd:cd17630 78 LAEDLAPPGVTHVSLVPTQLQRLLdsgQGPAALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTYGMTETaSQVATKRP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 329 PGKAKpGSSGKVVPGYDARIVDEnfqdipvgevGTLLIKGDSAAAAYWNKhQKTKSTMLGDWVNTDDKYAVDEEGYFYYI 408
Cdd:cd17630 157 DGFGR-GGVGVLLPGRELRIVED----------GEIWVGGASLAMGYLRG-QLVPEFNEDGWFTTKDLGELHADGRLTVL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 409 GRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlkkDLKEYVKKTLAHYKYP 488
Cdd:cd17630 225 GRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPA-----ELRAWLKDKLARFKLP 299
|
330 340
....*....|....*....|....
gi 700405679 489 RWIEFVDDLPKTATGKLKRFELRA 512
Cdd:cd17630 300 KRIYPVPELPRTGGGKVDRRALRA 323
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
40-442 |
1.40e-44 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 162.05 E-value: 1.40e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGL-GIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPIclNTLMqPKDYL-YFLNDSRAKILI 115
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAayVPL--DPAY-PAERLaFILEDAGARLLL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELYSKFEPIkdklrFLKHIIVVNGQAPAGTISFDSFVAgasdqleAAPITPDDACFWLYSSGSTGQPKG-------T 188
Cdd:TIGR01733 78 TDSALASRLAGL-----VLPVILLDPLELAALDDAPAPPPP-------DAPSGPDDLAYVIYTSGSTGRPKGvvvthrsL 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 189 IHLQHDMVYAAETYGKQVLgIQEHDICF--SAAKLFFayglgnglyfPFSVGATSVLMP---VRPVPASVYETIARYRPT 263
Cdd:TIGR01733 146 VNLLAWLARRYGLDPDDRV-LQFASLSFdaSVEEIFG----------ALLAGATLVVPPedeERDDAALLAALIAEHPVT 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQE-EGSLDGVRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTELS-----HIFISNLPGKAKPGS 336
Cdd:TIGR01733 215 VLNLTPSLLALLAAAlPPALASLRLVILGGEALTPALVDRWRARgPGARLINLYGPTETTvwstaTLVDPDDAPRESPVP 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 SGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTD---------DKYAVDEEGYFYY 407
Cdd:TIGR01733 295 IGRPLANTRLYVLDDDLRPVPVGVVGELYIGGPGVARGYLNRPELTAERFVPDPFAGGdgarlyrtgDLVRYLPDGNLEF 374
|
410 420 430
....*....|....*....|....*....|....*
gi 700405679 408 IGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAV 442
Cdd:TIGR01733 375 LGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
27-512 |
3.78e-44 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 163.29 E-value: 3.78e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRValLLLDSEFYPL--SFFGAIKIGAVPICLNTLMQPKDYLY 104
Cdd:PRK07470 21 PDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRI--LVHSRNCNQMfeSMFAAFRLGAVWVPTNFRQTPDEVAY 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVnGQAPAGTiSFDSFVAGASDQ-LEAAPITPDDACFWLYSSGSTG 183
Cdd:PRK07470 99 LAEASGARAMICHADFPEHAAAVRAASPDLTHVVAI-GGARAGL-DYEALVARHLGArVANAAVDHDDPCWFFFTSGTTG 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 184 QPKGTIHLQHDMVYAAETYGKQVL-GIQEHDICFSAAKLffAYGLGNGLYFPFSVGATSVLMPVRP-VPASVYETIARYR 261
Cdd:PRK07470 177 RPKAAVLTHGQMAFVITNHLADLMpGTTEQDASLVVAPL--SHGAGIHQLCQVARGAATVLLPSERfDPAEVWALVERHR 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 262 PTLYFGVPTLYgSMLQEEGSLDG-----VRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIfISNLPG------ 330
Cdd:PRK07470 255 VTNLFTVPTIL-KMLVEHPAVDRydhssLRYVIYAGAPMYRADQKRALAKLGKVLVQYFGLGEVTGN-ITVLPPalhdae 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 ---KAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYY 407
Cdd:PRK07470 333 dgpDARIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRDGWFRTGDLGHLDARGFLYI 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 408 IGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDdknlvkPK------AFVVLKRGYAPDDQlkkDLKEYVKKT 481
Cdd:PRK07470 413 TGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPD------PVwgevgvAVCVARDGAPVDEA---ELLAWLDGK 483
|
490 500 510
....*....|....*....|....*....|.
gi 700405679 482 LAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK07470 484 VARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
177-507 |
5.72e-44 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 157.95 E-value: 5.72e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIHLQHDMvyaAETYGKQVLGIQEH--DICFSAAKLFFAYGLgNGLYFPFSVGATSVLMPvRPVPASVY 254
Cdd:cd17633 7 FTSGTTGLPKAYYRSERSW---IESFVCNEDLFNISgeDAILAPGPLSHSLFL-YGAISALYLGGTFIGQR-KFNPKSWI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 255 ETIARYRPTLYFGVPTLYGSMLQEEGSLDGVRLCVSAGEALPADIFKRW-QQRHGVEIIDGIGSTELShiFIS-NLPGK- 331
Cdd:cd17633 82 RKINQYNATVIYLVPTMLQALARTLEPESKIKSIFSSGQKLFESTKKKLkNIFPKANLIEFYGTSELS--FITyNFNQEs 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 332 AKPGSSGKVVPGYDARIVDENFqdipvGEVGTLLIKGDSAAAAYWNKHQKTKstmlGDWVNTDDKYAVDEEGYFYYIGRS 411
Cdd:cd17633 160 RPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYVRGGFSNP----DGWMSVGDIGYVDEEGYLYLVGRE 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 412 NDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLkrgyapDDQLKKDLKEYVKKTLAHYKYPRWI 491
Cdd:cd17633 231 SDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYSG------DKLTYKQLKRFLKQKLSRYEIPKKI 304
|
330
....*....|....*.
gi 700405679 492 EFVDDLPKTATGKLKR 507
Cdd:cd17633 305 IFVDSLPYTSSGKIAR 320
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
64-511 |
1.50e-43 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 162.30 E-value: 1.50e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 64 NRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI-----------------IDEELYSKFEP 126
Cdd:PRK12492 76 DRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVylnmfgklvqevlpdtgIEYLIEAKMGD 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 127 IKDKLR-FLKHIIV--VNGQAPA----GTISFDSFV-AGASDQLEAAPITPDDACFWLYSSGSTGQPKGtihlqhdmvyA 198
Cdd:PRK12492 156 LLPAAKgWLVNTVVdkVKKMVPAyhlpQAVPFKQALrQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKG----------A 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 199 AETYGKQVLGIQEHDICFSAAKLFFAYGLGNG---------LYFPFSVGATSVLMPV-----------RPVPASVYEtIA 258
Cdd:PRK12492 226 MLTHGNLVANMLQVRACLSQLGPDGQPLMKEGqevmiaplpLYHIYAFTANCMCMMVsgnhnvlitnpRDIPGFIKE-LG 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 259 RYRPTLYFGVPTLYGSMLQEEG--SLDGVRLCV--SAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPG-KAK 333
Cdd:PRK12492 305 KWRFSALLGLNTLFVALMDHPGfkDLDFSALKLtnSGGTALVKATAERWEQLTGCTIVEGYGLTETSPVASTNPYGeLAR 384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 PGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSN 412
Cdd:PRK12492 385 LGTVGIPVPGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEgWFKTGDIAVIDPDGFVRIVDRKK 464
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 413 DMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKdlkeYVKKTLAHYKYPRWIE 492
Cdd:PRK12492 465 DLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKA----YCKENFTGYKVPKHIV 540
|
490
....*....|....*....
gi 700405679 493 FVDDLPKTATGKLKRFELR 511
Cdd:PRK12492 541 LRDSLPMTPVGKILRRELR 559
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
35-510 |
6.57e-43 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 159.21 E-value: 6.57e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 35 RDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPK--DYLYFLNDSRAK 112
Cdd:cd05923 25 RGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAelAELIERGEMTAA 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 113 ILIIDEelyskfEPIK-DKLRFLKHIIVvnGQAPaGTISFDSFvagaSDQLEAAPITPDDACFWLYSSGSTGQPKGTIHL 191
Cdd:cd05923 105 VIAVDA------QVMDaIFQSGVRVLAL--SDLV-GLGEPESA----GPLIEDPPREPEQPAFVFYTSGTTGLPKGAVIP 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 192 QHD-------MVYAAET-YGKQ--VLGIQE--HDICFSAakLFFAYGLGNGLYFP---FSvgatsvlmpvrpvPASVYET 256
Cdd:cd05923 172 QRAaesrvlfMSTQAGLrHGRHnvVLGLMPlyHVIGFFA--VLVAALALDGTYVVveeFD-------------PADALKL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVPTLYGSML-QEEGS---LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTElshIFISNLPGKA 332
Cdd:cd05923 237 IEQERVTSLFATPTHLDALAaAAEFAglkLSSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYGTTE---AMNSLYMRDA 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 KPGSSGKvvPGYD-----ARIVDENFQDIPVGEVGTLLIK--GDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYF 405
Cdd:cd05923 314 RTGTEMR--PGFFsevriVRIGGSPDEALANGEEGELIVAaaADAAFTGYLNQPEATAKKLQDGWYRTGDVGYVDPSGDV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEyvkKTLAHY 485
Cdd:cd05923 392 RILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSADELDQFCRA---SELADF 468
|
490 500
....*....|....*....|....*
gi 700405679 486 KYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd05923 469 KRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
29-507 |
8.58e-43 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 158.64 E-value: 8.58e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNTlmqPKDYL-YF 105
Cdd:cd17655 13 HTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGayLPIDPDY---PEERIqYI 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEELyskfepiKDKLRFLKHIIVVNGQapagtisfdSFVAGASDQLEAaPITPDDACFWLYSSGSTGQP 185
Cdd:cd17655 90 LEDSGADILLTQSHL-------QPPIAFIGLIDLLDED---------TIYHEESENLEP-VSKSDDLAYVIYTSGSTGKP 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTI--HLQ-HDMVYAAETYGKQvlgiQEHD-------ICF--SAAKLFFAYGLGNGLYfpfsVGATSVLMPVrpvpASV 253
Cdd:cd17655 153 KGVMieHRGvVNLVEWANKVIYQ----GEHLrvalfasISFdaSVTEIFASLLSGNTLY----IVRKETVLDG----QAL 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 YETIARYRPTLYFGVPTlYGSMLQEEGSLDG--VRLCVSAGEALPADIFKRW--QQRHGVEIIDGIGSTE-----LSHIF 324
Cdd:cd17655 221 TQYIRQNRITIIDLTPA-HLKLLDAADDSEGlsLKHLIVGGEALSTELAKKIieLFGTNPTITNAYGPTEttvdaSIYQY 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPGKAKPgSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLgdwvntDDKYAVDE--- 401
Cdd:cd17655 300 EPETDQQVSV-PIGKPLGNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFV------DDPFVPGErmy 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 402 ----------EGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPddqlk 471
Cdd:cd17655 373 rtgdlarwlpDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELPV----- 447
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 472 KDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17655 448 AQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDR 483
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
27-512 |
2.78e-42 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 158.19 E-value: 2.78e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSefyPLSF---FGAIKIGAVPICLNTLMQPKDYL 103
Cdd:PRK08162 32 PDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNI---PAMVeahFGVPMAGAVLNTLNTRLDAASIA 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 YFLNDSRAKILIIDEElYSKFepIKDKLRFLKH--IIVVN-------GQAPAGTISFDSFVAGASDQLEAAPitPDD--- 171
Cdd:PRK08162 109 FMLRHGEAKVLIVDTE-FAEV--AREALALLPGpkPLVIDvddpeypGGRFIGALDYEAFLASGDPDFAWTL--PADewd 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 172 ACFWLYSSGSTGQPKGTIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAyglgNGLYFPFSVGA---TSVLMpvRP 248
Cdd:PRK08162 184 AIALNYTSGTTGNPKGVVY-HHRGAYLNALSNILAWGMPKHPVYLWTLPMFHC----NGWCFPWTVAAragTNVCL--RK 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 V-PASVYETIARYRPTLYFGVPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQQRhGVEIIDGIGSTE---- 319
Cdd:PRK08162 257 VdPKLIFDLIREHGVTHYCGAPIVLSALInapaEWRAGIDHPVHAMVAGAAPPAAVIAKMEEI-GFDLTHVYGLTEtygp 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 320 ----LSHIFISNLPG------KAKPGSSGKVVPGydARIVD-ENFQDIP-----VGEV---GTLLIKGdsaaaaYWNKHQ 380
Cdd:PRK08162 336 atvcAWQPEWDALPLderaqlKARQGVRYPLQEG--VTVLDpDTMQPVPadgetIGEImfrGNIVMKG------YLKNPK 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 381 KTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVL 460
Cdd:PRK08162 408 ATEEAFAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVEL 487
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 700405679 461 KRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFvDDLPKTATGKLKRFELRA 512
Cdd:PRK08162 488 KDGASATEE---EIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQKFVLRE 535
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
28-507 |
5.08e-42 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 155.94 E-value: 5.08e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNTlmqPKDYL-Y 104
Cdd:cd12115 14 DAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAayVPLDPAY---PPERLrF 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQ 184
Cdd:cd12115 91 ILEDAQARLVLTD---------------------------------------------------PDDLAYVIYTSGSTGR 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGtIHLQHD-----MVYAAETYGK----QVLGIQEhdICF--SAAKLFFayglgnglyfPFSVGATSVL------MPVR 247
Cdd:cd12115 120 PKG-VAIEHRnaaafLQWAAAAFSAeelaGVLASTS--ICFdlSVFELFG----------PLATGGKVVLadnvlaLPDL 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 248 PVPASVyetiaryrpTLYFGVPTLYGSMLQEEGSLDGVRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTELSHIFIS 326
Cdd:cd12115 187 PAAAEV---------TLINTVPSAAAELLRHDALPASVRVVNLAGEPLPRDLVQRLYARlQVERVVNLYGPSEDTTYSTV 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NL--PGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-------WVNTDDKY 397
Cdd:cd12115 258 APvpPGASGEVSIGRPLANTQAYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDpfgpgarLYRTGDLV 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 398 AVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATDDKNLVkpkAFVVLKRGYAPDDQlkkDL 474
Cdd:cd12115 338 RWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVaigDAAGERRLV---AYIVAEPGAAGLVE---DL 411
|
490 500 510
....*....|....*....|....*....|...
gi 700405679 475 KEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd12115 412 RRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDR 444
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
30-512 |
1.91e-41 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 156.06 E-value: 1.91e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 30 TAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDS 109
Cdd:PRK06164 27 VALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRG 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 110 RAKILI-------IDeeLYSKFEPI-KDKLRFLKHIIVVNGQAPA--GTISFDSFVA-----GASDQLEAAPITPDDACF 174
Cdd:PRK06164 107 RARWLVvwpgfkgID--FAAILAAVpPDALPPLRAIAVVDDAADAtpAPAPGARVQLfalpdPAPPAAAGERAADPDAGA 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 175 WLYS-SGSTGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAYGLgNGLYFPFSVGATSVLMPVRPVPASV 253
Cdd:PRK06164 185 LLFTtSGTTSGPKLVLHRQATLLRHARAIA-RAYGYDPGAVLLAALPFCGVFGF-STLLGALAGGAPLVCEPVFDAARTA 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 yETIARYRPTLYFGVPTLYGSMLQEEGS---LDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFI---SN 327
Cdd:PRK06164 263 -RALRRHRVTHTFGNDEMLRRILDTAGEradFPSARLFGFASFAPALGELAALARARGVPLTGLYGSSEVQALVAlqpAT 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPGKAKPGSSGKVV-PGYDARIVDEnfQD---IPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEE 402
Cdd:PRK06164 342 DPVSVRIEGGGRPAsPEARVRARDP--QDgalLPDGESGEIEIRAPSLMRGYLDNPDATARALTDDgYFRTGDLGYTRGD 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 403 GYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVkPKAFVVLKRGYAPDDQlkkDLKEYVKKTL 482
Cdd:PRK06164 420 GQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATRDGKTV-PVAFVIPTDGASPDEA---GLMAACREAL 495
|
490 500 510
....*....|....*....|....*....|...
gi 700405679 483 AHYKYPRWIEFVDDLPKTATG---KLKRFELRA 512
Cdd:PRK06164 496 AGFKVPARVQVVEAFPVTESAngaKIQKHRLRE 528
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
27-512 |
3.89e-41 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 158.97 E-value: 3.89e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTlMQPKDYL-YF 105
Cdd:PRK12316 525 PEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDP-EYPAERLaYM 603
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 106 LNDSRAKILIIDEELyskfepiKDKLRFLKHIIVVNGQAPAgtisfdSFVAGASDQLEAAPITPDDACFWLYSSGSTGQP 185
Cdd:PRK12316 604 LEDSGVQLLLSQSHL-------GRKLPLAAGVQVLDLDRPA------AWLEGYSEENPGTELNPENLAYVIYTSGSTGKP 670
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGtIHLQHDMVYAAETYGKQVLGIQEHDICfsAAKLFFAYGLGNGLYF-PFSVGATSVLMPVRPV--PASVYETIARYRP 262
Cdd:PRK12316 671 KG-AGNRHRALSNRLCWMQQAYGLGVGDTV--LQKTPFSFDVSVWEFFwPLMSGARLVVAAPGDHrdPAKLVELINREGV 747
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQEEG--SLDGVRLCVSAGEALPADIFKR-WQQRHGVEIIDGIGSTE----LSHiFISNLPGKAKPg 335
Cdd:PRK12316 748 DTLHFVPSMLQAFLQDEDvaSCTSLRRIVCSGEALPADAQEQvFAKLPQAGLYNLYGPTEaaidVTH-WTCVEEGGDSV- 825
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 336 SSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVN------TDDKYAVDEEGYFYYI 408
Cdd:PRK12316 826 PIGRPIANLACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSpFVAgermyrTGDLARYRADGVIEYA 905
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 409 GRSNDMLKVGGIWVSPVEVEACLIEHPAVLEcAVVGATDDKNLVkpkAFVVLKrgyAPDDQLKKDLKEYVKKTLAHYKYP 488
Cdd:PRK12316 906 GRIDHQVKLRGLRIELGEIEARLLEHPWVRE-AAVLAVDGKQLV---GYVVLE---SEGGDWREALKAHLAASLPEYMVP 978
|
490 500
....*....|....*....|....
gi 700405679 489 RWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK12316 979 AQWLALERLPLTPNGKLDRKALPA 1002
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
38-511 |
8.30e-41 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 154.61 E-value: 8.30e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNAL-KGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILII 116
Cdd:PLN02574 66 SISYSELQPLVKSMAAGLyHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFT 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 DEELYSKFEPIKDKLRFLKHIIVVNGQAPAGTiSFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDMV 196
Cdd:PLN02574 146 SPENVEKLSPLGVPVIGVPENYDFDSKRIEFP-KFYELIKEDFDFVPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNLI 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 197 YAAETYGKQVLGIQEHDIC----FSAAKLFFAYGLGNGLYFPFSVGATSVLMpvRPVPAS-VYETIARYRPTLYFGVPTL 271
Cdd:PLN02574 225 AMVELFVRFEASQYEYPGSdnvyLAALPMFHIYGLSLFVVGLLSLGSTIVVM--RRFDASdMVKVIDRFKVTHFPVVPPI 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 YGSMLQEEGSLDG------VRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGK--AKPGSSGKVVPG 343
Cdd:PLN02574 303 LMALTKKAKGVCGevlkslKQVSCGAAPLSGKFIQDFVQTLPHVDFIQGYGMTESTAVGTRGFNTEklSKYSSVGLLAPN 382
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 344 YDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIW 421
Cdd:PLN02574 383 MQAKVVDwSTGCLLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDgWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQ 462
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 422 VSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTA 501
Cdd:PLN02574 463 IAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE---AVINYVAKQVAPYKKVRKVVFVQSIPKSP 539
|
490
....*....|
gi 700405679 502 TGKLKRFELR 511
Cdd:PLN02574 540 AGKILRRELK 549
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
36-508 |
5.43e-40 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 150.67 E-value: 5.43e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILi 115
Cdd:cd05914 5 GEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 ideelyskfepikdklrflkhiivvngqapagtisfdsFVagaSDqleaapitPDDACFWLYSSGSTGQPKGTIhLQHDM 195
Cdd:cd05914 84 --------------------------------------FV---SD--------EDDVALINYTSGTTGNSKGVM-LTYRN 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 196 VYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMpvRPVPASVYETIARYRPTLYFGVPTLY--- 272
Cdd:cd05914 114 IVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFL--DKIPSAKIIALAFAQVTPTLGVPVPLvie 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 273 ---------------------------------GSMLQEegSLDG-VRLCVSAGEALPADIfKRWQQRHGVEIIDGIGST 318
Cdd:cd05914 192 kifkmdiipkltlkkfkfklakkinnrkirklaFKKVHE--AFGGnIKEFVIGGAKINPDV-EEFLRTIGFPYTIGYGMT 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 319 ELSHIFISNLPGKAKPGSSGKVVPGYDARIVDENfqdiPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKY 397
Cdd:cd05914 269 ETAPIISYSPPNRIRLGSAGKVIDGVEVRIDSPD----PATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDgWFHTGDLG 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 398 AVDEEGYFYYIGRSNDMLKVG-GIWVSPVEVEACLIEHPAVLEcAVVGATDDKN--LVKPKA-FVVLKRGYAPD--DQLK 471
Cdd:cd05914 345 KIDAEGYLYIRGRKKEMIVLSsGKNIYPEEIEAKINNMPFVLE-SLVVVQEKKLvaLAYIDPdFLDVKALKQRNiiDAIK 423
|
490 500 510
....*....|....*....|....*....|....*...
gi 700405679 472 KDLKEYVKKTLAHY-KYPRWIEFVDDLPKTATGKLKRF 508
Cdd:cd05914 424 WEVRDKVNQKVPNYkKISKVKIVKEEFEKTPKGKIKRF 461
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
28-507 |
2.25e-39 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 148.55 E-value: 2.25e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTlMQPKDYLyfln 107
Cdd:cd05945 6 DRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDA-SSPAERI---- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 dsrAKILIIdeelyskfepikdklrflkhiivvngQAPAGTIsfdsfvagasdqleaapITPDDACFWLYSSGSTGQPKG 187
Cdd:cd05945 81 ---REILDA--------------------------AKPALLI-----------------ADGDDNAYIIFTSGSTGRPKG 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 tIHLQHDMVYAaetYGKQVLG---IQEHDICFSAAKLFFAYGLGnGLYFPFSVGATSVLMP--VRPVPASVYETIARYRP 262
Cdd:cd05945 115 -VQISHDNLVS---FTNWMLSdfpLGPGDVFLNQAPFSFDLSVM-DLYPALASGATLVPVPrdATADPKQLFRFLAEHGI 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQEE----GSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTEL----SHIFISNLP-GKA 332
Cdd:cd05945 190 TVWVSTPSFAAMCLLSPtftpESLPSLRHFLFCGEVLPHKTARALQQRFpDARIYNTYGPTEAtvavTYIEVTPEVlDGY 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 KPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD----WVNTDDKYAVDEEGYFYYI 408
Cdd:cd05945 270 DRLPIGYAKPGAKLVILDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDegqrAYRTGDLVRLEADGLLFYR 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 409 GRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGyaPDDQLKKDLKEYVKKTLAHYKYP 488
Cdd:cd05945 350 GRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPG--AEAGLTKAIKAELAERLPPYMIP 427
|
490
....*....|....*....
gi 700405679 489 RWIEFVDDLPKTATGKLKR 507
Cdd:cd05945 428 RRFVYLDELPLNANGKIDR 446
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
28-510 |
6.15e-39 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 147.80 E-value: 6.15e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-Y 104
Cdd:cd12114 2 DATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAayVPVDID---QPAARReA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEElyskfepikdklrflkhiIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQ 184
Cdd:cd12114 79 ILADAGARLVLTDGP------------------DAQLDVAVFDVLILDLDALAAPAPPPPVDVAPDDLAYVIFTSGSTGT 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGtIHLQHDMvyAAETYG--KQVLGIQEHDICFSAAKLFF---AYGLgnglyF-PFSVGATSVLmPV---RPVPASVYE 255
Cdd:cd12114 141 PKG-VMISHRA--ALNTILdiNRRFAVGPDDRVLALSSLSFdlsVYDI-----FgALSAGATLVL-PDearRRDPAHWAE 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGsML-----QEEGSLDGVRLCVSAGEALPADIFKRW-QQRHGVEIIDGIGSTELShIFiSNLP 329
Cdd:cd12114 212 LIERHGVTLWNSVPALLE-MLldvleAAQALLPSLRLVLLSGDWIPLDLPARLrALAPDARLISLGGATEAS-IW-SIYH 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 330 GKAKPGSSGKVVP------GYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-----WVNTDDKYA 398
Cdd:cd12114 289 PIDEVPPDWRSIPygrplaNQRYRVLDPRGRDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTHpdgerLYRTGDLGR 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 399 VDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV--GATDDKNLVkpkAFVVLKRGYAPDDQLkkDLKE 476
Cdd:cd12114 369 YRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVvlGDPGGKRLA---AFVVPDNDGTPIAPD--ALRA 443
|
490 500 510
....*....|....*....|....*....|....
gi 700405679 477 YVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd12114 444 FLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
165-505 |
9.01e-39 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 151.23 E-value: 9.01e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 165 APITPDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLM 244
Cdd:PRK08633 777 PTFKPDDTATIIFSSGSEGEPKG-VMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGLTVTLWLPLLEGIKVVYH 855
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 245 PvRPVPA-SVYETIARYRPTLYFGVPTLYGSMLQEE-------GSLdgvRLCVSAGEALPADIFKRWQQRHGVEIIDGIG 316
Cdd:PRK08633 856 P-DPTDAlGIAKLVAKHRATILLGTPTFLRLYLRNKklhplmfASL---RLVVAGAEKLKPEVADAFEEKFGIRILEGYG 931
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 317 STELSHIFISNLPGK----------AKPGSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKST 385
Cdd:PRK08633 932 ATETSPVASVNLPDVlaadfkrqtgSKEGSVGMPLPGVAVRIVDpETFEELPPGEDGLILIGGPQVMKGYLGDPEKTAEV 1011
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 386 M----LGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIE--HPAVLECAVVGATDDKnlvKPKAFVV 459
Cdd:PRK08633 1012 IkdidGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKalGGEEVVFAVTAVPDEK---KGEKLVV 1088
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 700405679 460 L-KRGYAPDDQLKKDLKEyvkKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:PRK08633 1089 LhTCGAEDVEELKRAIKE---SGLPNLWKPSRYFKVEALPLLGSGKL 1132
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
28-510 |
9.56e-39 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 151.65 E-value: 9.56e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK12316 2018 EAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLE 2097
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEPikdklrflkhiivvngqaPAGTISFDsfvAGASDQLEAAPIT-------PDDACFWLYSSG 180
Cdd:PRK12316 2098 DSGAALLLTQRHLLERLPL------------------PAGVARLP---LDRDAEWADYPDTapavqlaGENLAYVIYTSG 2156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 181 STGQPKGTI--H--LQHDMVYAAETYGKQVLGIQEHDICFSaaklfFAyGLGNGLYFPFSVGAtSVLMpvRP----VPAS 252
Cdd:PRK12316 2157 STGLPKGVAvsHgaLVAHCQAAGERYELSPADCELQFMSFS-----FD-GAHEQWFHPLLNGA-RVLI--RDdelwDPEQ 2227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARYRPTLyFGVPTLYGSMLQEEGSLDG----VRLCVSAGEALPADIFKRWQQR-HGVEIIDGIGSTE-----LSH 322
Cdd:PRK12316 2228 LYDEMERHGVTI-LDFPPVYLQQLAEHAERDGrppaVRVYCFGGEAVPAASLRLAWEAlRPVYLFNGYGPTEavvtpLLW 2306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 323 IFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTD 394
Cdd:PRK12316 2307 KCRPQDPCGAAYVPIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDpfsasgerLYRTG 2386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 395 DKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATdDKNLVkpkAFVVlkrgyaPDD--- 468
Cdd:PRK12316 2387 DLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVaqdGAS-GKQLV---AYVV------PDDaae 2456
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 700405679 469 QLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:PRK12316 2457 DLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKAL 2498
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
171-503 |
9.58e-39 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 143.98 E-value: 9.58e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 171 DACFWLYSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAyGLGNGLYFPFSVGATSVLMPvRPVP 250
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGAL-LSHQALLAQALVLAVLQAIDEGTVFLNSGPLFHI-GTLMFTLATFHAGGTNVFVR-RVDA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 251 ASVYETIARYRPTLYFGVPTLYGSMLQ--EEGSLDGVRLCVSAG-----EALPADIfKRWQQRHGveiidGIGSTELSHI 323
Cdd:cd17636 78 EEVLELIEAERCTHAFLLPPTIDQIVElnADGLYDLSSLRSSPAapewnDMATVDT-SPWGRKPG-----GYGQTEVMGL 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 324 FISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEG 403
Cdd:cd17636 152 ATFAALGGGAIGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRTRGGWHHTNDLGRREPDG 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 404 YFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLA 483
Cdd:cd17636 232 SLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEA---ELIEHCRARIA 308
|
330 340
....*....|....*....|
gi 700405679 484 HYKYPRWIEFVDDLPKTATG 503
Cdd:cd17636 309 SYKKPKSVEFADALPRTAGG 328
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
31-511 |
1.45e-37 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 144.41 E-value: 1.45e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPIclnTLMQPKDYLYF-LN 107
Cdd:cd17651 13 ALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAayVPL---DPAYPAERLAFmLA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEPIkdklrflkhiivvngqAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:cd17651 90 DAGPVLVLTHPALAGELAVE----------------LVAVTLLDQPGAAAGADAEPDPALDADDLAYVIYTSGSTGRPKG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 tIHLQHDMVyaaetygkqVLGIQEHD----ICFSAAKLFFAyglgnGLYFPFSV---------GATSVLMP--VRPVPAS 252
Cdd:cd17651 154 -VVMPHRSL---------ANLVAWQArassLGPGARTLQFA-----GLGFDVSVqeifstlcaGATLVLPPeeVRTDPPA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARYRPTLYFgVPTLYGSMLQEEGSLDGVRL-----CVSAGEALPADI-FKRWQQRH-GVEIIDGIGSTELSHIFI 325
Cdd:cd17651 219 LAAWLDEQRISRVF-LPTVALRALAEHGRPLGVRLaalryLLTGGEQLVLTEdLREFCAGLpGLRLHNHYGPTETHVVTA 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 326 SNLPGKAK----PGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVN------TD 394
Cdd:cd17651 298 LSLPGDPAawpaPPPIGRPIDNTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDpFVPgarmyrTG 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 395 DKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVG---ATDDKNLVkpkAFVVLKRGYAPDdqlK 471
Cdd:cd17651 378 DLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAredRPGEKRLV---AYVVGDPEAPVD---A 451
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 700405679 472 KDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd17651 452 AELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
18-511 |
1.42e-36 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 143.50 E-value: 1.42e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 18 VDRNIREGRGQKTAILCR------DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPI 91
Cdd:PLN02654 94 LDRNVEAGNGDKIAIYWEgnepgfDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHS 173
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 92 CLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIkdklrFLKHII-------VVNGQAPAGTISFDS----------- 153
Cdd:PLN02654 174 VVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTI-----NLKDIVdaaldesAKNGVSVGICLTYENqlamkredtkw 248
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 154 ----------FVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHD-MVYAAETYgKQVLGIQEHDICFSAAKLF 222
Cdd:PLN02654 249 qegrdvwwqdVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMVYTATTF-KYAFDYKPTDVYWCTADCG 327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 223 FAYGLGNGLYFPFSVGATSVLM---PVRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEG------SLDGVRLCVSAGE 293
Cdd:PLN02654 328 WITGHSYVTYGPMLNGATVLVFegaPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDeyvtrhSRKSLRVLGSVGE 407
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 294 ALPADIFKRWQQRHG---VEIIDGIGSTELSHIFISNLPGK--AKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKG 368
Cdd:PLN02654 408 PINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKK 487
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 369 DSAAA--AYWNKHQKTKSTMLGDWVN---TDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV 443
Cdd:PLN02654 488 SWPGAfrTLYGDHERYETTYFKPFAGyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVV 567
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 444 GATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PLN02654 568 GIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 635
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
39-483 |
4.01e-35 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 137.60 E-value: 4.01e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 39 FTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID- 117
Cdd:cd05932 7 FTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVGk 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 ------------EELYSKFEPIKDKLRFLKhiivvngqapagtiSFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQP 185
Cdd:cd05932 87 lddwkamapgvpEGLISISLPPPSAANCQY--------------QWDDLIAQHPPLEERPTRFPEQLATLIYTSGTTGQP 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTIHLQHDMVYAAETyGKQVLGIQEHDICFS-------AAKLFFAYGL---GNGLYFPFSVGatsvlmpvrpvpaSVYE 255
Cdd:cd05932 153 KGVMLTFGSFAWAAQA-GIEHIGTEENDRMLSylplahvTERVFVEGGSlygGVLVAFAESLD-------------TFVE 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLY-------------------------GSMLQEEG----SLDGVRLCVSAGEALPADIFKrWQQR 306
Cdd:cd05932 219 DVQRARPTLFFSVPRLWtkfqqgvqdkipqqklnlllkipvvNSLVKRKVlkglGLDQCRLAGCGSAPVPPALLE-WYRS 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 307 HGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVDEnfqdipvgevGTLLIKGDSAAAAYWNKHQKTKSTM 386
Cdd:cd05932 298 LGLNILEAYGMTENFAYSHLNYPGRDKIGTVGNAGPGVEVRISED----------GEILVRSPALMMGYYKDPEATAEAF 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 LGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVG-GIWVSPVEVEACLIEHPAVLECAVVGAtddkNLVKPKAFVVLKRGY 464
Cdd:cd05932 368 TADgFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIGS----GLPAPLALVVLSEEA 443
|
490 500
....*....|....*....|.
gi 700405679 465 AP--DDQLKKDLKEYVKKTLA 483
Cdd:cd05932 444 RLraDAFARAELEASLRAHLA 464
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
26-507 |
9.98e-35 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 135.92 E-value: 9.98e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLL-DSEFYPLsFFGAIKIGAVPI-CLNTLMQpKDYL 103
Cdd:cd05920 28 HPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPnVAEFVVL-FFALLRLGAVPVlALPSHRR-SELS 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 YFLNDSRAKILIIDEElYSKFEPikdklRFLKHiivvngqapagtisfdsfvagasdqlEAAPITPDDACFWLySSGSTG 183
Cdd:cd05920 106 AFCAHAEAVAYIVPDR-HAGFDH-----RALAR--------------------------ELAESIPEVALFLL-SGGTTG 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 184 QPKGTIHLQHDMVYAAETyGKQVLGIQEHDICFSAAKLFFAYGL-GNGLYFPFSVGATSVLMPvRPVPASVYETIARYRP 262
Cdd:cd05920 153 TPKLIPRTHNDYAYNVRA-SAEVCGLDQDTVYLAVLPAAHNFPLaCPGVLGTLLAGGRVVLAP-DPSPDAAFPLIEREGV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQEEGSLD------------GVRLCVSAGEALPADIFKRWQQRHGveIIDG-IGSTEL--SHIFISN 327
Cdd:cd05920 231 TVTALVPALVSLWLDAAASRRadlsslrllqvgGARLSPALARRVPPVLGCTLQQVFG--MAEGlLNYTRLddPDEVIIH 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPGKakPGSsgkvvPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFY 406
Cdd:cd05920 309 TQGR--PMS-----PDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDgFYRTGDLVRRTPDGYLV 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 407 YIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLkRGYAPD-DQLKKDLKEyvkKTLAHY 485
Cdd:cd05920 382 VEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVL-RDPPPSaAQLRRFLRE---RGLAAY 457
|
490 500
....*....|....*....|..
gi 700405679 486 KYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd05920 458 KLPDRIEFVDSLPLTAVGKIDK 479
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
40-512 |
2.34e-34 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 136.45 E-value: 2.34e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDE 118
Cdd:PRK05620 40 TFAAIGARAAALAHALHDeLGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVADP 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFEPIKDKLRFLKHIIVVNG--------QAPAG--TISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGt 188
Cdd:PRK05620 120 RLAEQLGEILKECPCVRAVVFIGPsdadsaaaHMPEGikVYSYEALLDGRSTVYDWPELDETTAAAICYSTGTTGAPKG- 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 189 ihlqhdMVYAAETYGKQVLGIQEHDicfsaaklffAYGLGNGLYF--------------PFS--VGATSVLMPVRPV-PA 251
Cdd:PRK05620 199 ------VVYSHRSLYLQSLSLRTTD----------SLAVTHGESFlccvpiyhvlswgvPLAafMSGTPLVFPGPDLsAP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 252 SVYETIARYRPTLYFGVPTLYGSML-------QEEGSLdgvRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIF 324
Cdd:PRK05620 263 TLAKIIATAMPRVAHGVPTLWIQLMvhylknpPERMSL---QEIYVGGSAVPPILIKAWEERYGVDVVHVWGMTETSPVG 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 I-----SNLPGKAKPG---SSGKVVPGYDARIVDEnfqdipvGEV--------GTLLIKGDSAAAAYWNKHQKTK----S 384
Cdd:PRK05620 340 TvarppSGVSGEARWAyrvSQGRFPASLEYRIVND-------GQVmestdrneGEIQVRGNWVTASYYHSPTEEGggaaS 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 385 TMLGD-------------WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNL 451
Cdd:PRK05620 413 TFRGEdvedandrftadgWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWG 492
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 452 VKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK05620 493 ERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQ 553
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
1-507 |
2.97e-34 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 137.99 E-value: 2.97e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 1 MHVELNLPETfNAADYFVDRNIREGRGQ---KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYP 77
Cdd:PRK12467 498 ELVRWNAPAT-EYAPDCVHQLIEAQARQhpeRPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMV 576
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 78 LSFFGAIKIGAVPICLNTlMQPKDYL-YFLNDSRAKILIIDEELYSKFePIKDKLRFLkhiiVVNGQAPagtisfdsFVA 156
Cdd:PRK12467 577 VGLLAVLKAGGAYVPLDP-EYPQDRLaYMLDDSGVRLLLTQSHLLAQL-PVPAGLRSL----CLDEPAD--------LLC 642
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 157 GASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGlGNGLYFPFS 236
Cdd:PRK12467 643 GYSGHNPEVALDPDNLAYVIYTSGSTGQPKG-VAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLG-VTELFGALA 720
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 237 VGATSVLMPVRPV--PASVYETIARYRPTLYFGVPTLYGSMLQEEGSlDGVRLC---VSAGEALPADIFKRWQQ-RHGVE 310
Cdd:PRK12467 721 SGATLHLLPPDCArdAEAFAALMADQGVTVLKIVPSHLQALLQASRV-ALPRPQralVCGGEALQVDLLARVRAlGPGAR 799
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 311 IIDGIGSTE----LSHIFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTM 386
Cdd:PRK12467 800 LINHYGPTEttvgVSTYELSDEERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERF 879
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 L--------------GDWVntddKYAVDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAV--VGATDDKN 450
Cdd:PRK12467 880 VpdpfgadggrlyrtGDLA----RYRAD--GVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVlaQPGDAGLQ 953
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 451 LVkpkAFVVLKRGyaPDDQ----LKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK12467 954 LV---AYLVPAAV--ADGAehqaTRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDR 1009
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
30-510 |
5.17e-34 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 133.15 E-value: 5.17e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 30 TAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-YFL 106
Cdd:cd17652 4 PAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAayLPLDPA---YPAERIaYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 107 NDSRAKILIIdeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGSTGQPK 186
Cdd:cd17652 81 ADARPALLLT---------------------------------------------------TPDNLAYVIYTSGSTGRPK 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 187 GTIhLQH----DMVYAAETY-----GKQVLgiQEHDICFSAAKLFFAYGLGNGlyfpfsvgATSVLMPVRPV--PASVYE 255
Cdd:cd17652 110 GVV-VTHrglaNLAAAQIAAfdvgpGSRVL--QFASPSFDASVWELLMALLAG--------ATLVLAPAEELlpGEPLAD 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGSMlqEEGSLDGVRLCVSAGEALPADIFKRWQQRHgvEIIDGIGSTE--LSHIFISNLPGKAK 333
Cdd:cd17652 179 LLREHRITHVTLPPAALAAL--PPDDLPDLRTLVVAGEACPAELVDRWAPGR--RMINAYGPTEttVCATMAGPLPGGGV 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 PgSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTDDKYAVDEEGYF 405
Cdd:cd17652 255 P-PIGRPVPGTRVYVLDARLRPVPPGVPGELYIAGAGLARGYLNRPGLTAERFVADpfgapgsrMYRTGDLARWRADGQL 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLEcAVVGATDD----KNLVkpkAFVVLKRGYAPDdqlKKDLKEYVKKT 481
Cdd:cd17652 334 EFLGRADDQVKIRGFRIELGEVEAALTEHPGVAE-AVVVVRDDrpgdKRLV---AYVVPAPGAAPT---AAELRAHLAER 406
|
490 500
....*....|....*....|....*....
gi 700405679 482 LAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd17652 407 LPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
176-512 |
1.00e-33 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 135.08 E-value: 1.00e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 LYSSGSTGQPKGtihLQHDM---VYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVL---MPVRPV 249
Cdd:PRK10524 239 LYTSGTTGKPKG---VQRDTggyAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMyegLPTRPD 315
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 250 PASVYETIARYRPTLYFGVPTL------YGSMLQEEGSLDGVRLCVSAGEAL--PAdifKRWQQRH-GVEIIDGIGSTEL 320
Cdd:PRK10524 316 AGIWWRIVEKYKVNRMFSAPTAirvlkkQDPALLRKHDLSSLRALFLAGEPLdePT---ASWISEAlGVPVIDNYWQTET 392
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 321 SHIFISNLPG----KAKPGSSGKVVPGYDARIVDENF-QDIPVGEVGTLLIK------------GDSA--AAAYWnKH-- 379
Cdd:PRK10524 393 GWPILAIARGvedrPTRLGSPGVPMYGYNVKLLNEVTgEPCGPNEKGVLVIEgplppgcmqtvwGDDDrfVKTYW-SLfg 471
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 380 QKTKSTMlgDWVntddkyAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD-KNLVkPKAFV 458
Cdd:PRK10524 472 RQVYSTF--DWG------IRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDAlKGQV-AVAFV 542
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 700405679 459 VLKRGYAPDDQ-----LKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK10524 543 VPKDSDSLADRearlaLEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQA 601
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
31-507 |
2.95e-33 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 135.08 E-value: 2.95e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSR 110
Cdd:PRK12316 3075 ALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSG 3154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 111 AKILIIDEELYskfepikdklrfLKHIIVVNgqapagTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIH 190
Cdd:PRK12316 3155 AQLLLSQSHLR------------LPLAQGVQ------VLDLDRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKG-VG 3215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 LQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAyGLGNGLYFPFSVGATSVLMPVR--PVPASVYETIARYRPTLYFGV 268
Cdd:PRK12316 3216 IRHSALSNHLCWMQQAYGLGVGDRVLQFTTFSFD-VFVEELFWPLMSGARVVLAGPEdwRDPALLVELINSEGVDVLHAY 3294
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 269 PTLYGSMLQEEG--SLDGVRLCVSAGEALPADIFKRWQQrhGVEIIDGIGSTELSHIFISNLPGKAKPGSS--GKVVPGY 344
Cdd:PRK12316 3295 PSMLQAFLEEEDahRCTSLKRIVCGGEALPADLQQQVFA--GLPLYNLYGPTEATITVTHWQCVEEGKDAVpiGRPIANR 3372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 345 DARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-------WVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK12316 3373 ACYILDGSLEPVPVGALGELYLGGEGLARGYHNRPGLTAERFVPDpfvpgerLYRTGDLARYRADGVIEYIGRVDHQVKI 3452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLEcAVVGATDDKNLVkpkAFVVLKrgyAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PRK12316 3453 RGFRIELGEIEARLLEHPWVRE-AVVLAVDGRQLV---AYVVPE---DEAGDLREALKAHLKASLPEYMVPAHLLFLERM 3525
|
490
....*....|
gi 700405679 498 PKTATGKLKR 507
Cdd:PRK12316 3526 PLTPNGKLDR 3535
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
176-507 |
4.04e-33 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 128.92 E-value: 4.04e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 LYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATsVLMPVRPVPASVYE 255
Cdd:cd17635 7 IFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLC-VTGGENTTYKSLFK 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPT-------LYGSMLQEEGSLDgvrlCVSAGEALPADIFKRWQQRHG-VEIIDGIGSTELSHI-FIS 326
Cdd:cd17635 86 ILTTNAVTTTCLVPTllsklvsELKSANATVPSLR----LIGYGGSRAIAADVRFIEATGlTNTAQVYGLSETGTAlCLP 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFY 406
Cdd:cd17635 162 TDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLIDGWVNTGDLGERREDGFLF 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 407 YIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVgATDDKNLVKPKAFVVLKRGYApDDQLKKDLKEYVKKTLAHYK 486
Cdd:cd17635 242 ITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACY-EISDEEFGELVGLAVVASAEL-DENAIRALKHTIRRELEPYA 319
|
330 340
....*....|....*....|.
gi 700405679 487 YPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17635 320 RPSTIVIVTDIPRTQSGKVKR 340
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
28-507 |
5.55e-33 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 134.02 E-value: 5.55e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKT----AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTlMQPKDYL 103
Cdd:PRK10252 469 AKTpdapALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDT-GYPDDRL 547
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 -YFLNDSRAKILIIDEELYSKFePIKDKLRFLkhiivvngqapagtiSFDSFVAGASDQLEAAPiTPDDACFWLYSSGST 182
Cdd:PRK10252 548 kMMLEDARPSLLITTADQLPRF-ADVPDLTSL---------------CYNAPLAPQGAAPLQLS-QPHHTAYIIFTSGST 610
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 183 GQPKGTIHLQHDMVyaaetygKQVLGIQEHdicfsaaklffaYGLGNG-----------------LYFPFSVGATSVLMP 245
Cdd:PRK10252 611 GRPKGVMVGQTAIV-------NRLLWMQNH------------YPLTADdvvlqktpcsfdvsvweFFWPFIAGAKLVMAE 671
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 --VRPVPASVYETIARYR-PTLYFgVPTLYGSMLQEEGSLDGVRLCVS------AGEALPADIFKRWQQRHGVEIIDGIG 316
Cdd:PRK10252 672 peAHRDPLAMQQFFAEYGvTTTHF-VPSMLAAFVASLTPEGARQSCASlrqvfcSGEALPADLCREWQQLTGAPLHNLYG 750
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 317 STELShIFISNLP--GKAKPGSSGKVVP-GYDA-----RIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLG 388
Cdd:PRK10252 751 PTEAA-VDVSWYPafGEELAAVRGSSVPiGYPVwntglRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIA 829
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 389 D-------WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAV-----LEC----AVVGATDDKNLV 452
Cdd:PRK10252 830 DpfapgerMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVeqavtHACvinqAAATGGDARQLV 909
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 700405679 453 kpkAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK10252 910 ---GYLVSQSGLPLD---TSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDR 958
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
31-507 |
1.84e-32 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 132.77 E-value: 1.84e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNtLMQPKDYL-YFLNDS 109
Cdd:PRK12316 4569 AVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLD-PEYPRERLaYMMEDS 4647
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 110 RAKILIIDEELYSKFePIKDKLRFLkhiivvngqapagTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtI 189
Cdd:PRK12316 4648 GAALLLTQSHLLQRL-PIPDGLASL-------------ALDRDEDWEGFPAHDPAVRLHPDNLAYVIYTSGSTGRPKG-V 4712
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 HLQHD-----MVYAAETYGkqvLGIQEHDICFSAaklfFAY-GLGNGLYFPFSVGAtSVLMPVRPV--PASVYETIARYR 261
Cdd:PRK12316 4713 AVSHGslvnhLHATGERYE---LTPDDRVLQFMS----FSFdGSHEGLYHPLINGA-SVVIRDDSLwdPERLYAEIHEHR 4784
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 262 PTLYFGVPTLYGSMLQ---EEGSLDGVRLCVSAGEALPADIFKR-WQQRHGVEIIDGIGSTELSHIFIS-NLPGKAKPGS 336
Cdd:PRK12316 4785 VTVLVFPPVYLQQLAEhaeRDGEPPSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTVTVLLwKARDGDACGA 4864
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 S----GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTDDKYAVDEEGY 404
Cdd:PRK12316 4865 AympiGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDpfgapggrLYRTGDLARYRADGV 4944
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV---GATdDKNLVkpkAFVV-----LKRGYAPDDQLKKDLKE 476
Cdd:PRK12316 4945 IDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIaqeGAV-GKQLV---GYVVpqdpaLADADEAQAELRDELKA 5020
|
490 500 510
....*....|....*....|....*....|.
gi 700405679 477 YVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK12316 5021 ALRERLPEYMVPAHLVFLARMPLTPNGKLDR 5051
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
38-511 |
4.33e-31 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 126.63 E-value: 4.33e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:PLN02330 55 AVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVTN 134
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKFEPIKdklrflKHIIVVNGQAPAGTISFDSFVAGA---SDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHD 194
Cdd:PLN02330 135 DTNYGKVKGLG------LPVIVLGEEKIEGAVNWKELLEAAdraGDTSDNEEILQTDLCALPFSSGTTGISKGVMLTHRN 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 195 MV----YAAETYGKQVLGiqeHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPVPASVYETIAR---YRPTLYFG 267
Cdd:PLN02330 209 LVanlcSSLFSVGPEMIG---QVVTLGLIPFFHIYGITGICCATLRNKGKVVVMSRFELRTFLNALITQevsFAPIVPPI 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 268 VPTLYGSMLQEEGSLDGVRL--CVSAGEALPADIFKRWQQRH-GVEIIDGIGSTE-----LSHIFISNLPGKAKPGSSGK 339
Cdd:PLN02330 286 ILNLVKNPIVEEFDLSKLKLqaIMTAAAPLAPELLTAFEAKFpGVQVQEAYGLTEhscitLTHGDPEKGHGIAKKNSVGF 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 340 VVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PLN02330 366 ILPNLEVKFIDpDTGRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDgWLHTGDIGYIDDDGDIFIVDRIKELIKY 445
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PLN02330 446 KGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSI 522
|
490
....*....|....
gi 700405679 498 PKTATGKLKRFELR 511
Cdd:PLN02330 523 PKSLSGKIMRRLLK 536
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
6-512 |
5.68e-31 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 126.50 E-value: 5.68e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 6 NLPEtfNAADY-------FVDRNIREgRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPL 78
Cdd:PLN02479 9 DLPK--NAANYtaltplwFLERAAVV-HPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYE 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 79 SFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPI------KDKLRFLKHIIVVNG---QAPA--- 146
Cdd:PLN02479 86 AHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVDQEFFTLAEEAlkilaeKKKSSFKPPLLIVIGdptCDPKslq 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 147 -----GTISFDSFVAGASDQLEAAPitPDDAcfWL-----YSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICF 216
Cdd:PLN02479 166 yalgkGAIEYEKFLETGDPEFAWKP--PADE--WQsialgYTSGTTASPKGVV-LHHRGAYLMALSNALIWGMNEGAVYL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 217 SAAKLFFAyglgNGLYFPFSVGA---TSVLMpvRPVPA-SVYETIARYRPTLYFGVPTLYGSML---QEEGSLDGVRL-- 287
Cdd:PLN02479 241 WTLPMFHC----NGWCFTWTLAAlcgTNICL--RQVTAkAIYSAIANYGVTHFCAAPVVLNTIVnapKSETILPLPRVvh 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 288 CVSAGEALPADIFKRWQQRhGVEIIDGIGSTE----------------LSHIFISNLpgKAKPGSSGKVVPGYDarIVD- 350
Cdd:PLN02479 315 VMTAGAAPPPSVLFAMSEK-GFRVTHTYGLSEtygpstvcawkpewdsLPPEEQARL--NARQGVRYIGLEGLD--VVDt 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 351 ENFQDIPV--GEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVE 428
Cdd:PLN02479 390 KTMKPVPAdgKTMGEIVMRGNMVMKGYLKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVE 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 429 ACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQ--LKKDLKEYVKKTLAHYKYPRWIEFvDDLPKTATGKLK 506
Cdd:PLN02479 470 NVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDEaaLAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQ 548
|
....*.
gi 700405679 507 RFELRA 512
Cdd:PLN02479 549 KHVLRA 554
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
27-512 |
1.06e-30 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 125.76 E-value: 1.06e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 27 GQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFL 106
Cdd:PRK08279 51 PDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGAVLAHSL 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 107 NDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNG---QAPAGTISFDSFVAGASDQLEAA--PITPDDACFWLYSSGS 181
Cdd:PRK08279 131 NLVDAKHLIVGEELVEAFEEARADLARPPRLWVAGGdtlDDPEGYEDLAAAAAGAPTTNPASrsGVTAKDTAFYIYTSGT 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 182 TGQPKGTIHLQHDMVYAAETYGkQVLGIQEHDICFSAAKLFFAYGLgnglyfpfSVGATSVLMPVRPV------PAS-VY 254
Cdd:PRK08279 211 TGLPKAAVMSHMRWLKAMGGFG-GLLRLTPDDVLYCCLPLYHNTGG--------TVAWSSVLAAGATLalrrkfSASrFW 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 255 ETIARYRPTLyFGvptlY-GSML-----QEEGSLDG---VRLCVSAGeaLPADIFKRWQQRHGVE-IIDGIGSTElSHIF 324
Cdd:PRK08279 282 DDVRRYRATA-FQ----YiGELCryllnQPPKPTDRdhrLRLMIGNG--LRPDIWDEFQQRFGIPrILEFYAASE-GNVG 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ISNLPGkaKPGSSGKvVPGYDA---RIV-----------DEN--FQDIPVGEVGtLLIKGDSAAAAYW--NKHQKTKSTM 386
Cdd:PRK08279 354 FINVFN--FDGTVGR-VPLWLAhpyAIVkydvdtgepvrDADgrCIKVKPGEVG-LLIGRITDRGPFDgyTDPEASEKKI 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 L------GD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAV----VGATDDKnlvKPK 455
Cdd:PRK08279 430 LrdvfkkGDaWFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGVEEAVVygveVPGTDGR---AGM 506
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 700405679 456 AFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK08279 507 AAIVLADGAEFD---LAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRK 560
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
35-510 |
1.24e-30 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 125.10 E-value: 1.24e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 35 RDNAFTYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKI 113
Cdd:cd05938 2 EGETYTYRDVDRRSNQAARALLAhAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 114 LIIDEELYSKFEPIKDKLRflKHIIVV----NGQAPAGTISFDSFVAGASDQLE----AAPITPDDACFWLYSSGSTGQP 185
Cdd:cd05938 82 LVVAPELQEAVEEVLPALR--ADGVSVwylsHTSNTEGVISLLDKVDAASDEPVpaslRAHVTIKSPALYIYTSGTTGLP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTIHLQHDMVYAAETYgkQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPvrPVPAS-VYETIARYRPT- 263
Cdd:cd05938 160 KAARISHLRVLQCSGFL--SLCGVTADDVIYITLPLYHSSGFLLGIGGCIELGATCVLKP--KFSASqFWDDCRKHNVTv 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 -LYFGVPTLY-GSMLQEEGSLD-GVRLcvSAGEALPADIFKRWQQRHG-VEIIDGIGSTELSHIFIsNLPGkaKPGSSGK 339
Cdd:cd05938 236 iQYIGELLRYlCNQPQSPNDRDhKVRL--AIGNGLRADVWREFLRRFGpIRIREFYGSTEGNIGFF-NYTG--KIGAVGR 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 340 V-------VP----GYDAR----IVDENFQDIPV--GEVGTLLIKGDSAAA--AYWNKHQKTKSTMLGD-------WVNT 393
Cdd:cd05938 311 VsylykllFPfeliKFDVEkeepVRDAQGFCIPVakGEPGLLVAKITQQSPflGYAGDKEQTEKKLLRDvfkkgdvYFNT 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 394 DDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATddknlvKP-------KAFVVLKRGYAP 466
Cdd:cd05938 391 GDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVYGVT------VPghegrigMAAVKLKPGHEF 464
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 700405679 467 DdqlKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd05938 465 D---GKKLYQHVREYLPAYARPRFLRIQDSLEITGTFKQQKVRL 505
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
28-505 |
2.94e-30 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 122.89 E-value: 2.94e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLG-IEMENRVALLLLDSEFYPLSFFGAIKIGA--VPIclnTLMQPKDYLY 104
Cdd:cd17648 2 DRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAayVPI---DPSYPDERIQ 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 F-LNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTG 183
Cdd:cd17648 79 FiLEDTGARVVITN---------------------------------------------------STDLAYAIYTSGTTG 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 184 QPKGtIHLQHDMVYAAETYGKQVLGIQEHDicfSAAKLFFAyglgnGLYFPFSV---------GATSVLMP--VRPVPAS 252
Cdd:cd17648 108 KPKG-VLVEHGSVVNLRTSLSERYFGRDNG---DEAVLFFS-----NYVFDFFVeqmtlallnGQKLVVPPdeMRFDPDR 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 253 VYETIARYRPTLYFGVPtlygSMLQ--EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELS--HIFISNL 328
Cdd:cd17648 179 FYAYINREKVTYLSGTP----SVLQqyDLARLPHLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTETTvtNHKRFFP 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 329 PGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFY-- 406
Cdd:cd17648 255 GDQRFDKSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTEQERARGRNARLYkt 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 407 -------------YIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV--------GATDDKNLVkpkAFVVLKRGYA 465
Cdd:cd17648 335 gdlvrwlpsgeleYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVakedasqaQSRIQKYLV---GYYLPEPGHV 411
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 700405679 466 PDdqlkKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:cd17648 412 PE----SDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKL 447
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
28-510 |
1.44e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 121.64 E-value: 1.44e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK13383 50 GRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAAALR 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEPIKDKlrflkhIIVVngqapagtisfDSFVAGASDQlEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK13383 130 AHHISTVVADNEFAERIAGADDA------VAVI-----------DPATAGAEES-GGRPAVAAPGRIVLLTSGTTGKPKG 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIHLQHdmvyAAETYGKQVLgIQEHDICFSAAKLFFA----YGLGNG-LYFPFSVGATSVLMPVRPVPASVYETiARYRP 262
Cdd:PRK13383 192 VPRAPQ----LRSAVGVWVT-ILDRTRLRTGSRISVAmpmfHGLGLGmLMLTIALGGTVLTHRHFDAEAALAQA-SLHRA 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQ------EEGSLDGVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELShifISNLPGKAK--- 333
Cdd:PRK13383 266 DAFTAVPVVLARILElpprvrARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVG---IGALATPADlrd 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 -PGSSGKVVPGYDARIVDENFQdiPVGE--VGTLLIKGDSAAAAYWNKHQKTkstMLGDWVNTDDKYAVDEEGYFYYIGR 410
Cdd:PRK13383 343 aPETVGKPVAGCPVRILDRNNR--PVGPrvTGRIFVGGELAGTRYTDGGGKA---VVDGMTSTGDMGYLDNAGRLFIVGR 417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 411 SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDdqlKKDLKEYVKKTLAHYKYPRW 490
Cdd:PRK13383 418 EDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVD---AAQLRDYLKDRVSRFEQPRD 494
|
490 500
....*....|....*....|
gi 700405679 491 IEFVDDLPKTATGKLKRFEL 510
Cdd:PRK13383 495 INIVSSIPRNPTGKVLRKEL 514
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
28-507 |
8.70e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 121.42 E-value: 8.70e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK12467 1589 EAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIE 1668
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFePIKDKLRflkhiIVVNGQApagtisfDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK12467 1669 DSGIELLLTQSHLQARL-PLPDGLR-----SLVLDQE-------DDWLEGYSDSNPAVNLAPQNLAYVIYTSGSTGRPKG 1735
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIHLQHDMV----YAAETYGkqvlgiqehdicFSAA-----KLFFAYGLGN-GLYFPFSVGATSVLMP--VRPVPASVYE 255
Cdd:PRK12467 1736 AGNRHGALVnrlcATQEAYQ------------LSAAdvvlqFTSFAFDVSVwELFWPLINGARLVIAPpgAHRDPEQLIQ 1803
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTLYGSMLQEEGSLDG---VRLCVSAGEALPADIFKRWQQRHG-VEIIDGIGSTE----LSHIFISN 327
Cdd:PRK12467 1804 LIERQQVTTLHFVPSMLQQLLQMDEQVEHplsLRRVVCGGEALEVEALRPWLERLPdTGLFNLYGPTEtavdVTHWTCRR 1883
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPGKAKPGSS-GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDD----------K 396
Cdd:PRK12467 1884 KDLEGRDSVPiGQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFGTVGsrlyrtgdlaR 1963
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 397 YAVDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVgATDDKNLVKPKAFVVLKRGYAPDDQ-----LK 471
Cdd:PRK12467 1964 YRAD--GVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVI-AQDGANGKQLVAYVVPTDPGLVDDDeaqvaLR 2040
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 472 KDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK12467 2041 AILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDR 2076
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
29-511 |
9.67e-29 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 119.74 E-value: 9.67e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:PLN03102 30 RTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRH 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDEElyskFEP-IKDKLRFLK--------HIIVVNG-QAPAGTISFDSFVAGASDQLEAAPIT---------P 169
Cdd:PLN03102 110 AKPKILFVDRS----FEPlAREVLHLLSsedsnlnlPVIFIHEiDFPKRPSSEELDYECLIQRGEPTPSLvarmfriqdE 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 170 DDACFWLYSSGSTGQPKGTIhLQHDMVYAAETygKQVLGiQEHDIC---FSAAKLFFAyglgNGLYFPFSV---GATSVL 243
Cdd:PLN03102 186 HDPISLNYTSGTTADPKGVV-ISHRGAYLSTL--SAIIG-WEMGTCpvyLWTLPMFHC----NGWTFTWGTaarGGTSVC 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 244 MpvRPVPA-SVYETIARYRPTLYFGVPTLYgSMLQEEGSLD-----GVRLCVSAGEALPADIFKRwQQRHGVEIIDGIGS 317
Cdd:PLN03102 258 M--RHVTApEIYKNIEMHNVTHMCCVPTVF-NILLKGNSLDlsprsGPVHVLTGGSPPPAALVKK-VQRLGFQVMHAYGL 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 318 TELSHIFI--------SNLPG------KAKPGSSGKVVPGYDARiVDENFQDIPVG--EVGTLLIKGDSAAAAYWNKHQK 381
Cdd:PLN03102 334 TEATGPVLfcewqdewNRLPEnqqmelKARQGVSILGLADVDVK-NKETQESVPRDgkTMGEIVIKGSSIMKGYLKNPKA 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 382 TKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLK 461
Cdd:PLN03102 413 TSEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVVLE 492
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 700405679 462 RG----YAPDDQL---KKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:PLN03102 493 KGettkEDRVDKLvtrERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLR 549
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
31-507 |
1.46e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 121.04 E-value: 1.46e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-YFLN 107
Cdd:PRK12467 3113 ALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGayVPLDPE---YPRERLaYMIE 3189
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFePIKDKLrflkHIIVVNGQAPAGTisfdsfvagaSDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK12467 3190 DSGVKLLLTQAHLLEQL-PAPAGD----TALTLDRLDLNGY----------SENNPSTRVMGENLAYVIYTSGSTGKPKG 3254
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 --------TIHLQhdmvYAAETYGkqvLGIQEHDICFSAaklfFAY-GLGNGLYFPFSVGATSVLMPVRP-VPASVYETI 257
Cdd:PRK12467 3255 vgvrhgalANHLC----WIAEAYE---LDANDRVLLFMS----FSFdGAQERFLWTLICGGCLVVRDNDLwDPEELWQAI 3323
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFGVPTLYGSMLQEEGSLDG--VRLCVSAGEALPADIFKRWQqRH--GVEIIDGIGSTELSHIFIS-NLPGKA 332
Cdd:PRK12467 3324 HAHRISIACFPPAYLQQFAEDAGGADCasLDIYVFGGEAVPPAAFEQVK-RKlkPRGLTNGYGPTEAVVTVTLwKCGGDA 3402
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 KPGSS----GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD--------WVNTDDKYAVD 400
Cdd:PRK12467 3403 VCEAPyapiGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADpfsgsggrLYRTGDLARYR 3482
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 401 EEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLEcAVVGATDD---KNLVkpkAFVVLKrgyAPDDQLKKDLKEY 477
Cdd:PRK12467 3483 ADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVRE-AVVLARDGaggKQLV---AYVVPA---DPQGDWRETLRDH 3555
|
490 500 510
....*....|....*....|....*....|
gi 700405679 478 VKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK12467 3556 LAASLPDYMVPAQLLVLAAMPLGPNGKVDR 3585
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
65-512 |
3.32e-28 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 118.11 E-value: 3.32e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 65 RVALLL---LDsefYPLSFFGAIKIGAVPIclnTLMQPKDYLYF------LNDSRAKILIIDEELyskfepiKDKLRflK 135
Cdd:cd05931 50 RVLLLAppgLD---FVAAFLGCLYAGAIAV---PLPPPTPGRHAerlaaiLADAGPRVVLTTAAA-------LAAVR--A 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 136 HIIVVNGQAPAGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDIC 215
Cdd:cd05931 115 FAASRPAAGTPRLLVVDLLPDTSAADWPPPSPDPDDIAYLQYTSGSTGTPKGVV-VTHRNLLANVRQIRRAYGLDPGDVV 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 216 FSAAKLFFAYGLGNGLYFPFSVGATSVLMPvrPV-----PASVYETIARYRPTlYFGVPT----LYGSMLQEE--GSLD- 283
Cdd:cd05931 194 VSWLPLYHDMGLIGGLLTPLYSGGPSVLMS--PAaflrrPLRWLRLISRYRAT-ISAAPNfaydLCVRRVRDEdlEGLDl 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 284 -GVRLCVSAGEALPADIFKRWQQR------------------------------HGVeIIDGIGSTELSHIFisNLPGKA 332
Cdd:cd05931 271 sSWRVALNGAEPVRPATLRRFAEAfapfgfrpeafrpsyglaeatlfvsggppgTGP-VVLRVDRDALAGRA--VAVAAD 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 333 KPG-----SSGKVVPGYDARIVDEN-FQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML-------GDWVNTDDkYAV 399
Cdd:cd05931 348 DPAarelvSCGRPLPDQEVRIVDPEtGRELPDGEVGEIWVRGPSVASGYWGRPEATAETFGalaatdeGGWLRTGD-LGF 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 400 DEEGYFYYIGRSNDMLKVGG--IWvsPVEVEACLIEHPAVL--ECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkKDLK 475
Cdd:cd05931 427 LHDGELYITGRLKDLIIVRGrnHY--PQDIEATAEEAHPALrpGCVAAFSVPDDGEERLVVVAEVERGADPADL--AAIA 502
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 700405679 476 EYVKKTLA--HYKYPRWIEFV--DDLPKTATGKLKRFELRA 512
Cdd:cd05931 503 AAIRAAVAreHGVAPADVVLVrpGSIPRTSSGKIQRRACRA 543
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
36-511 |
5.04e-28 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 116.30 E-value: 5.04e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitpddACFWLYSSGSTGQPKGTIHLQHDM 195
Cdd:cd05940 81 VD------------------------------------------------------AALYIYTSGTTGLPKAAIISHRRA 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 196 VYAAETYGKQVLGIQEhDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMpvRPVPAS-VYETIARYRPTL--YFGVPTLY 272
Cdd:cd05940 107 WRGGAFFAGSGGALPS-DVLYTCLPLYHSTALIVGWSACLASGATLVIR--KKFSASnFWDDIRKYQATIfqYIGELCRY 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 273 gSMLQEEGSLD-GVRLCVSAGEALPADIFKRWQQRHGV-EIIDGIGSTELSHIFIsNLPGKakPGSSGKVVP----GYDA 346
Cdd:cd05940 184 -LLNQPPKPTErKHKVRMIFGNGLRPDIWEEFKERFGVpRIAEFYAATEGNSGFI-NFFGK--PGAIGRNPSllrkVAPL 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 347 RIV--DENFQD-----------IPVGEVGTLL--IKGDSAAAAYWNKHQKTKSTML-----GD-WVNTDDKYAVDEEGYF 405
Cdd:cd05940 260 ALVkyDLESGEpirdaegrcikVPRGEPGLLIsrINPLEPFDGYTDPAATEKKILRdvfkkGDaWFNTGDLMRLDGEGFW 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 406 YYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAV----VGATDDKnlvKPKAFVVLKRGYAPDdqLKKdLKEYVKKT 481
Cdd:cd05940 340 YFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygvqVPGTDGR---AGMAAIVLQPNEEFD--LSA-LAAHLEKN 413
|
490 500 510
....*....|....*....|....*....|
gi 700405679 482 LAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05940 414 LPGYARPLFLRLQPEMEITGTFKQQKVDLR 443
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
26-512 |
5.95e-28 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 119.12 E-value: 5.95e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTlMQPKDYL-Y 104
Cdd:PRK05691 2201 TPQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDP-EYPLERLhY 2279
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLNDSRAKILIIDEELYSKFepikdklrflkhiivvnGQAPAGTISF----DSFVAGASDQLEAAPIT-PDDACFWLYSS 179
Cdd:PRK05691 2280 MIEDSGIGLLLSDRALFEAL-----------------GELPAGVARWcledDAAALAAYSDAPLPFLSlPQHQAYLIYTS 2342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 180 GSTGQPKGTI--HLQHDMVYAA--ETYGKQVLGIQEH--DICFSAAKlffayglgNGLYFPFSVGATSVLMPVRPVPASV 253
Cdd:PRK05691 2343 GSTGKPKGVVvsHGEIAMHCQAviERFGMRADDCELHfySINFDAAS--------ERLLVPLLCGARVVLRAQGQWGAEE 2414
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 254 YETIARYRPTLYFGVPTLYGSMLQE----EGSLDGVRLCVSAGEALPADIFKRWQQRHGVEII-DGIGSTE-----LSHI 323
Cdd:PRK05691 2415 ICQLIREQQVSILGFTPSYGSQLAQwlagQGEQLPVRMCITGGEALTGEHLQRIRQAFAPQLFfNAYGPTEtvvmpLACL 2494
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 324 FISNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTD--------D 395
Cdd:PRK05691 2495 APEQLEEGAASVPIGRVVGARVAYILDADLALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFAADggrlyrtgD 2574
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 396 KYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLEcAVVGATDD---KNLVKPKAFVVLKRGYAPDDQLKK 472
Cdd:PRK05691 2575 LVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVRE-AVVLALDTpsgKQLAGYLVSAVAGQDDEAQAALRE 2653
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 700405679 473 DLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK05691 2654 ALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRALPA 2693
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
58-512 |
1.64e-27 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 116.05 E-value: 1.64e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 58 LGIEMENRVALLLLDSEFYPLSFFgaikigAVPiCLNTLMQPKDYLYFLNDS-------RAKILIIDEELYSKFEPIK-D 129
Cdd:PLN02860 52 LGLRNGDVVAIAALNSDLYLEWLL------AVA-CAGGIVAPLNYRWSFEEAksamllvRPVMLVTDETCSSWYEELQnD 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 130 KLRFLKHIIVVNGQAPAGTISFDSFV--------AGASDQLEAApITPDDACFWLYSSGSTGQPKGTIhLQHDMVYAAET 201
Cdd:PLN02860 125 RLPSLMWQVFLESPSSSVFIFLNSFLttemlkqrALGTTELDYA-WAPDDAVLICFTSGTTGRPKGVT-ISHSALIVQSL 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 202 YGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFpFSVGATSVLMPvRPVPASVYETIARYRPTLYFGVPTLYGSML---QE 278
Cdd:PLN02860 203 AKIAIVGYGEDDVYLHTAPLCHIGGLSSALAM-LMVGACHVLLP-KFDAKAALQAIKQHNVTSMITVPAMMADLIsltRK 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 279 EGSLDG---VRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTEL--SHIFIS-------------NLPGKAKPGSS-- 337
Cdd:PLN02860 281 SMTWKVfpsVRKILNGGGSLSSRLLPDAKKLFpNAKLFSAYGMTEAcsSLTFMTlhdptlespkqtlQTVNQTKSSSVhq 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 ------GKVVPGYDARIVdenfQDIPVgEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGR 410
Cdd:PLN02860 361 pqgvcvGKPAPHVELKIG----LDESS-RVGRILTRGPHVMLGYWGQNSETASVLSNDgWLDTGDIGWIDKAGNLWLIGR 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 411 SNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKD-----------LKEYV- 478
Cdd:PLN02860 436 SNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGWIWSDNEKENakknltlssetLRHHCr 515
|
490 500 510
....*....|....*....|....*....|....*
gi 700405679 479 KKTLAHYKYPR-WIEFVDDLPKTATGKLKRFELRA 512
Cdd:PLN02860 516 EKNLSRFKIPKlFVQWRKPFPLTTTGKIRRDEVRR 550
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
31-507 |
1.13e-26 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 112.56 E-value: 1.13e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMqPKDYL-YFLNDS 109
Cdd:cd17650 5 AVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDY-PAERLqYMLEDS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 110 RAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKGTI 189
Cdd:cd17650 84 GAKLLLTQ---------------------------------------------------PEDLAYVIYTSGTTGKPKGVM 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 ----HLQHdMVYAAET-YG---KQVLGIQEHDICFSAAKLFFAYGLGNGlyfpfsvgATSVLMP--VRPVPASVYETIAR 259
Cdd:cd17650 113 vehrNVAH-AAHAWRReYEldsFPVRLLQMASFSFDVFAGDFARSLLNG--------GTLVICPdeVKLDPAALYDLILK 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 260 YRPTLYFGVPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQQRHG--VEIIDGIGSTEL---SHIFISNLPG 330
Cdd:cd17650 184 SRITLMESTPALIRPVMayvyRNGLDLSAMRLLIVGSDGCKAQDFKTLAARFGqgMRIINSYGVTEAtidSTYYEEGRDP 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 KAKPGSS--GKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-------WVNTDDKYAVDE 401
Cdd:cd17650 264 LGDSANVpiGRPLPNTAMYVLDERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENpfapgerMYRTGDLARWRA 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 402 EGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVlkrgyaPDDQLK-KDLKEYVKK 480
Cdd:cd17650 344 DGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV------AAATLNtAELRAFLAK 417
|
490 500
....*....|....*....|....*..
gi 700405679 481 TLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17650 418 ELPSYMIPSYYVQLDALPLTPNGKVDR 444
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
31-507 |
1.50e-26 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 112.14 E-value: 1.50e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNtlmQPKDYL-YFLN 107
Cdd:cd17644 18 AVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGayVPLDPN---YPQERLtYILE 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKG 187
Cdd:cd17644 95 DAQISVLLTQ---------------------------------------------------PENLAYVIYTSGSTGKPKG 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIhLQHDMVYAAETYGKQVLGIQEHD-------ICFSAAklffayglGNGLYFPFSVGATSVLMP--VRPVPASVYETIA 258
Cdd:cd17644 124 VM-IEHQSLVNLSHGLIKEYGITSSDrvlqfasIAFDVA--------AEEIYVTLLSGATLVLRPeeMRSSLEDFVQYIQ 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 259 RYRPTLyFGVPTLYGSMLQEEGSL------DGVRLCVSAGEALPADIFKRWQQRHG--VEIIDGIGSTELS-----HIFI 325
Cdd:cd17644 195 QWQLTV-LSLPPAYWHLLVLELLLstidlpSSLRLVIVGGEAVQPELVRQWQKNVGnfIQLINVYGPTEATiaatvCRLT 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 326 SNLPGKAKPGSSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVN---------TDD- 395
Cdd:cd17644 274 QLTERNITSVPIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFNsseserlykTGDl 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 396 -KYAVDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATD---DKNLVkpkAFVVLKRGYAPDdqlK 471
Cdd:cd17644 354 aRYLPD--GNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDqpgNKRLV---AYIVPHYEESPS---T 425
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 472 KDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:cd17644 426 VELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDR 461
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
31-510 |
1.77e-26 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 112.18 E-value: 1.77e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 31 AILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSR 110
Cdd:cd17656 6 AVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSG 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 111 AKILIIDEELyskfepiKDKLRFLKHIIVVNgqapagtisfDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGTI- 189
Cdd:cd17656 86 VRVVLTQRHL-------KSKLSFNKSTILLE----------DPSISQEDTSNIDYINNSDDLLYIIYTSGTTGKPKGVQl 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 -H------LQHDMVYAAETYGKQVLGIQ--EHDICFSaaKLFFAYGLGNGLYfpfsvgatsvLMP---VRPVPaSVYETI 257
Cdd:cd17656 149 eHknmvnlLHFEREKTNINFSDKVLQFAtcSFDVCYQ--EIFSTLLSGGTLY----------IIReetKRDVE-QLFDLV 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFgVPTLYGSMLQEEGSL-----DGVRLCVSAGEALP-ADIFKRWQQRHGVEIIDGIGSTElSHIF------- 324
Cdd:cd17656 216 KRHNIEVVF-LPVAFLKFIFSEREFinrfpTCVKHIITAGEQLViTNEFKEMLHEHNVHLHNHYGPSE-THVVttytinp 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 325 ---ISNLPGKAKPGSSGKVVpgydarIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-------WVNTD 394
Cdd:cd17656 294 eaeIPELPPIGKPISNTWIY------ILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDpfdpnerMYRTG 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 395 DKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGyAPDDQlkkdL 474
Cdd:cd17656 368 DLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFVMEQE-LNISQ----L 442
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 475 KEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd17656 443 REYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
26-512 |
1.88e-26 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 112.25 E-value: 1.88e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPIclnTLMQPKDYL 103
Cdd:cd05918 12 QPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGafVPL---DPSHPLQRL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 -YFLNDSRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGST 182
Cdd:cd05918 89 qEILQDTGAKVVLTS--------------------------------------------------SPSDAAYVIFTSGST 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 183 GQPKGTIhLQHDMVY-AAETYGKQvLGIQEHDICFsaakLFFAYG----LGNgLYFPFSVGATSVLMPVRPVPASVYETI 257
Cdd:cd05918 119 GKPKGVV-IEHRALStSALAHGRA-LGLTSESRVL----QFASYTfdvsILE-IFTTLAAGGCLCIPSEEDRLNDLAGFI 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFGVPTLyGSMLQEEgSLDGVRLCVSAGEALPADIFKRWQQRhgVEIIDGIGSTELSHIFISNLPG-KAKPGS 336
Cdd:cd05918 192 NRLRVTWAFLTPSV-ARLLDPE-DVPSLRTLVLGGEALTQSDVDTWADR--VRLINAYGPAECTIAATVSPVVpSTDPRN 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 SGKVVPGyDARIVDENFQDI--PVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVN--------------TDD--KYA 398
Cdd:cd05918 268 IGRPLGA-TCWVVDPDNHDRlvPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPAWlkqegsgrgrrlyrTGDlvRYN 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 399 VDeeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV------GATDDKNLVkpkAFVVLKRG--------- 463
Cdd:cd05918 347 PD--GSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVevvkpkDGSSSPQLV---AFVVLDGSssgsgdgds 421
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 700405679 464 --YAPDDQLK---KDLKEYVKKTLAHYKYPR-WIeFVDDLPKTATGKLKRFELRA 512
Cdd:cd05918 422 lfLEPSDEFRalvAELRSKLRQRLPSYMVPSvFL-PLSHLPLTASGKIDRRALRE 475
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
28-512 |
3.87e-26 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 113.34 E-value: 3.87e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK05691 3735 QRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIE 3814
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEPIKDKLrflkhiivvNGQAPAGTISFDSFVAGASDQleAAP---ITPDDACFWLYSSGSTGQ 184
Cdd:PRK05691 3815 LSRTPVLVCSAACREQARALLDEL---------GCANRPRLLVWEEVQAGEVAS--HNPgiySGPDNLAYVIYTSGSTGL 3883
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 185 PKGTIHLQHDMVyaaetyGKQV-----LGIQEHDICFSAAKLFFAYGLGNGLYFPFsVGATSVLMP--VRPVPASVYETI 257
Cdd:PRK05691 3884 PKGVMVEQRGML------NNQLskvpyLALSEADVIAQTASQSFDISVWQFLAAPL-FGARVEIVPnaIAHDPQGLLAHV 3956
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ARYRPTLYFGVPTLYGSML-QEEGSLDGVRLCVSAGEALPADIFKRWQQRH-GVEIIDGIGSTELS------HIFISNLP 329
Cdd:PRK05691 3957 QAQGITVLESVPSLIQGMLaEDRQALDGLRWMLPTGEAMPPELARQWLQRYpQIGLVNAYGPAECSddvaffRVDLASTR 4036
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 330 GKAKPGSSgkvvPGYDAR--IVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML-------GDWV-NTDDKYAV 399
Cdd:PRK05691 4037 GSYLPIGS----PTDNNRlyLLDEALELVPLGAVGELCVAGTGVGRGYVGDPLRTALAFVphpfgapGERLyRTGDLARR 4112
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 400 DEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAV--VGATDDKNLVkpkAFVVLKRGYAPDDQLKKDLKEY 477
Cdd:PRK05691 4113 RSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVavQEGVNGKHLV---GYLVPHQTVLAQGALLERIKQR 4189
|
490 500 510
....*....|....*....|....*....|....*
gi 700405679 478 VKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK05691 4190 LRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPA 4224
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
66-512 |
9.72e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 110.54 E-value: 9.72e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 66 VALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDeelySKFEPIKDKLrfLKHIIVVNGQAP 145
Cdd:PRK07867 57 VGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDIAHADCQLVLTE----SAHAELLDGL--DPGVRVINVDSP 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 146 AgtisFDSFVAGASD-QLEAAPITPDDACFWLYSSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFA 224
Cdd:PRK07867 131 A----WADELAAHRDaEPPFRVADPDDLFMLIFTSGTSGDPKAVR-CTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHS 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 225 YGLGNGLYFPFSVGATSVLMpvRPVPASVY-ETIARYRPTL--YFGVPTLYgsMLQEEGSLDG----VRLcVSAGEALPA 297
Cdd:PRK07867 206 NAVMAGWAVALAAGASIALR--RKFSASGFlPDVRRYGATYanYVGKPLSY--VLATPERPDDadnpLRI-VYGNEGAPG 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 298 DIfKRWQQRHGVEIIDGIGSTELShIFISNLPGkAKPGSSGKVVPGydARIVD-ENFQDIPVGE------------VGTL 364
Cdd:PRK07867 281 DI-ARFARRFGCVVVDGFGSTEGG-VAITRTPD-TPPGALGPLPPG--VAIVDpDTGTECPPAEdadgrllnadeaIGEL 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 365 L-IKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV 443
Cdd:PRK07867 356 VnTAGPGGFEGYYNDPEADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVY 435
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 444 GATDDKNLVKPKAFVVLKRGYAPD-DQLKKDLKEyvKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK07867 436 AVPDPVVGDQVMAALVLAPGAKFDpDAFAEFLAA--QPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSA 503
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
23-512 |
1.10e-25 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 110.46 E-value: 1.10e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 23 REGRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEmENRVALLLLD--SEFYpLSFFGAIKIGAVPIclNTL---- 96
Cdd:PRK10946 33 RHAASDAIAVICGERQFSYRELNQASDNLACSLRRQGIK-PGDTALVQLGnvAEFY-ITFFALLKLGVAPV--NALfshq 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 97 ----------MQPKdylyFLNDSRAKILIIDEELYSKFepiKDKLRFLKHIIVVNGQapaGTISFDSFVAGASDQLEAAP 166
Cdd:PRK10946 109 rselnayasqIEPA----LLIADRQHALFSDDDFLNTL---VAEHSSLRVVLLLNDD---GEHSLDDAINHPAEDFTATP 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 167 ITPDDACFWLYSSGSTGQPKgTIHLQHDmvyaaeTYGKQVLGIQEhdICFSAAKLFFAYGLGNGLYFPFS---------V 237
Cdd:PRK10946 179 SPADEVAFFQLSGGSTGTPK-LIPRTHN------DYYYSVRRSVE--ICGFTPQTRYLCALPAAHNYPMSspgalgvflA 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 238 GATSVLMPvRPVPASVYETIARYRPTLYFGVP---TLYGSMLQEEG------SLD-----GVRLCVSAGEALPADIFKRW 303
Cdd:PRK10946 250 GGTVVLAP-DPSATLCFPLIEKHQVNVTALVPpavSLWLQAIAEGGsraqlaSLKllqvgGARLSETLARRIPAELGCQL 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 304 QQRHGVEiiDG-IGSTELS----HIFISnlpgKAKPGSsgkvvPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNK 378
Cdd:PRK10946 329 QQVFGMA--EGlVNYTRLDdsdeRIFTT----QGRPMS-----PDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKS 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 379 HQKTKSTM-------LGDWVntddkyAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNL 451
Cdd:PRK10946 398 PQHNASAFdangfycSGDLV------SIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMG 471
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 700405679 452 VKPKAFVVLKRGYAPdDQLKKDLKEyvkKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK10946 472 EKSCAFLVVKEPLKA-VQLRRFLRE---QGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQ 528
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
154-512 |
1.17e-25 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 110.50 E-value: 1.17e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 154 FVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFayglGNGLYF 233
Cdd:PRK13388 134 LVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKA-VRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFH----SNAVMA 208
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 234 PFSV----GATSVLmPVRPVPASVYETIARYRPTL--YFGVPTLYgSMLQEEGSLDGV-RLCVSAG-EALPADIfKRWQQ 305
Cdd:PRK13388 209 GWAPavasGAAVAL-PAKFSASGFLDDVRRYGATYfnYVGKPLAY-ILATPERPDDADnPLRVAFGnEASPRDI-AEFSR 285
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 306 RHGVEIIDGIGSTELSHIFIsnLPGKAKPGSSGKVVPGydARIVD-ENFQDIPVGE-------------VGTLLIKGDSA 371
Cdd:PRK13388 286 RFGCQVEDGYGSSEGAVIVV--REPGTPPGSIGRGAPG--VAIYNpETLTECAVARfdahgallnadeaIGELVNTAGAG 361
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 372 A-AAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKN 450
Cdd:PRK13388 362 FfEGYYNNPEATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERV 441
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 451 LVKPKAFVVLKRG--YAPDdqlkkDLKEYV--KKTLAHYKYPRWIEFVDDLPKTATGKLKRFELRA 512
Cdd:PRK13388 442 GDQVMAALVLRDGatFDPD-----AFAAFLaaQPDLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
426-504 |
5.88e-25 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 98.00 E-value: 5.88e-25
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 700405679 426 EVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPddqLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVEL---LEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
30-511 |
8.47e-25 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 106.62 E-value: 8.47e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 30 TAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGA--VPICLNTLMQPKDYLyfLN 107
Cdd:cd17653 14 VAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAayVPLDAKLPSARIQAI--LR 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGSTGQPKG 187
Cdd:cd17653 92 TSGATLLLTTD-------------------------------------------------SPDDLAYIIFTSGSTGIPKG 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 tIHLQHDMVYAAETYGKQVLGIQEHDicfSAAKLF-FAYGLGNGLYFP-FSVGATSVLMPvrpvPASVYETIARYRPTLY 265
Cdd:cd17653 123 -VMVPHRGVLNYVSQPPARLDVGPGS---RVAQVLsIAFDACIGEIFStLCNGGTLVLAD----PSDPFAHVARTVDALM 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 266 FgVPTLYgSMLQEEgSLDGVRLCVSAGEALPADIFKRWqqRHGVEIIDGIGSTE--LSHIFISNLPGkaKPGSSGKVVPG 343
Cdd:cd17653 195 S-TPSIL-STLSPQ-DFPNLKTIFLGGEAVPPSLLDRW--SPGRRLYNAYGPTEctISSTMTELLPG--QPVTIGKPIPN 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 344 YDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVN-------TDDKYAVDEEGYFYYIGRSNDMLK 416
Cdd:cd17653 268 STCYILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPFWpgsrmyrTGDYGRWTEDGGLEFLGREDNQVK 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 417 VGGIWVSPVEVEACLIEHPAVLECAVVGATDDkNLVkpkAFVvlkrgyAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDD 496
Cdd:cd17653 348 VRGFRINLEEIEEVVLQSQPEVTQAAAIVVNG-RLV---AFV------TPETVDVDGLRSELAKHLPSYAVPDRIIALDS 417
|
490
....*....|....*
gi 700405679 497 LPKTATGKLKRFELR 511
Cdd:cd17653 418 FPLTANGKVDRKALR 432
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
166-504 |
1.03e-24 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 108.26 E-value: 1.03e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 166 PITPDDACFWLYSSGSTGQPKGTIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMP 245
Cdd:PRK08043 361 KQQPEDAALILFTSGSEGHPKGVVH-SHKSLLANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYP 439
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 ----VRPVPASVYETiaryRPTLYFGVPTLYGSMLQEEGSLDGVRL--CVSAGEALPADIFKRWQQRHGVEIIDGIGSTE 319
Cdd:PRK08043 440 splhYRIVPELVYDR----NCTVLFGTSTFLGNYARFANPYDFARLryVVAGAEKLQESTKQLWQDKFGLRILEGYGVTE 515
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 320 LSHIFISNLPGKAKPGSSGKVVPGYDARIvdenfqdIPVGEV---GTLLIKGDSAAAAYWN----------KHQKTKSTM 386
Cdd:PRK08043 516 CAPVVSINVPMAAKPGTVGRILPGMDARL-------LSVPGIeqgGRLQLKGPNIMNGYLRvekpgvlevpTAENARGEM 588
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 LGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEA-CLIEHPAVLECAVvgATDDKNlvKPKAFVVlkrgYA 465
Cdd:PRK08043 589 ERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQlALGVSPDKQHATA--IKSDAS--KGEALVL----FT 660
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 700405679 466 PDDQLKKD-LKEYVKKT-LAHYKYPRWIEFVDDLPKTATGK 504
Cdd:PRK08043 661 TDSELTREkLQQYAREHgVPELAVPRDIRYLKQLPLLGSGK 701
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
38-480 |
2.12e-24 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 105.90 E-value: 2.12e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd17640 5 RITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVVE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapiTPDDACFWLYSSGSTGQPKGTI--H--LQH 193
Cdd:cd17640 85 N-------------------------------------------------DSDDLATIIYTSGTTGNPKGVMltHanLLH 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMVYAAE----TYGKQVLGIQEhdICFSAAKLFfayglgngLYFPFSVGATSVLMPVRPVPasvyETIARYRPTLYFGVP 269
Cdd:cd17640 116 QIRSLSDivppQPGDRFLSILP--IWHSYERSA--------EYFIFACGCSQAYTSIRTLK----DDLKRVKPHYIVSVP 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 270 TLYGSM---LQEEGS-------------LDG--VRLCVSAGEALP--ADIFKRwqqRHGVEIIDGIGSTELSHIFISNLP 329
Cdd:cd17640 182 RLWESLysgIQKQVSksspikqflflffLSGgiFKFGISGGGALPphVDTFFE---AIGIEVLNGYGLTETSPVVSARRL 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 330 GKAKPGSSGKVVPGYDARIVDENFQDI-PVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYY 407
Cdd:cd17640 259 KCNVRGSVGRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDgWFNTGDLGWLTCGGELVL 338
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 700405679 408 IGRSNDMLKV-GGIWVSPVEVEACLIEHPAVLECAVVGaTDDKNLvkpKAFVVlkrgyaPD-DQLKKDLKEYVKK 480
Cdd:cd17640 339 TGRAKDTIVLsNGENVEPQPIEEALMRSPFIEQIMVVG-QDQKRL---GALIV------PNfEELEKWAKESGVK 403
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
36-511 |
3.42e-24 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 105.59 E-value: 3.42e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:cd05915 22 VHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELYSKFEpikDKLRFLKHIiVVNGQAPAGTISFDSFVAGASDQLEaaPITPDDACFWL---YSSGSTGQPKGTIHLQ 192
Cdd:cd05915 102 FDPNLLPLVE---AIRGELKTV-QHFVVMDEKAPEGYLAYEEALGEEA--DPVRVPERAACgmaYTTGTTGLPKGVVYSH 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 193 HDMVYAAETYG-KQVLGIQEHDICFSAAKLFFAyglgNGLYFPFSVGA-TSVLMPVRPVPAS--VYETIARYRPTLYFGV 268
Cdd:cd05915 176 RALVLHSLAASlVDGTALSEKDVVLPVVPMFHV----NAWCLPYAATLvGAKQVLPGPRLDPasLVELFDGEGVTFTAGV 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 269 PTLYGSMLQEEGSLDGV---RLCVSAGEALPADIFKRWQQ--------RHGVEIIDGIGSTELSHIFISNLPG------K 331
Cdd:cd05915 252 PTVWLALADYLESTGHRlktLRRLVVGGSAAPRSLIARFErmgvevrqGYGLTETSPVVVQNFVKSHLESLSEeekltlK 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 332 AKPGSSgKVVPGYDarIVDENFQDIPVG--EVGTLLIKGDSAAAAYWNKHQKTKSTML-GDWVNTDDKYAVDEEGYFYYI 408
Cdd:cd05915 332 AKTGLP-IPLVRLR--VADEEGRPVPKDgkALGEVQLKGPWITGGYYGNEEATRSALTpDGFFRTGDIAVWDEEGYVEIK 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 409 GRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDQlkkdLKEYVKKTLAHYKY- 487
Cdd:cd05915 409 DRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPEE----LNEHLLKAGFAKWQl 484
|
490 500
....*....|....*....|....
gi 700405679 488 PRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05915 485 PDAYVFAEEIPRTSAGKFLKRALR 508
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
22-444 |
1.51e-23 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 103.83 E-value: 1.51e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 22 IREGRGQKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDS-EFYPLSFfgAI-KIGAVPICLNTLMQP 99
Cdd:PRK09274 25 VAVPGGRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSlEFFALTF--ALfKAGAVPVLVDPGMGI 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 100 KDYLYFLNDSRAKILI-IdeelyskfePIKDKLRFL--------KHIIVVNGQAPAGTISFDSFVAGASD-QLEAAPITP 169
Cdd:PRK09274 103 KNLKQCLAEAQPDAFIgI---------PKAHLARRLfgwgkpsvRRLVTVGGRLLWGGTTLATLLRDGAAaPFPMADLAP 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 170 DDACFWLYSSGSTGQPKGTIHLqHDMVYAAETYGKQVLGIQEHDICFSAAKLFfayGLgnglyFPFSVGATSVL--M-PV 246
Cdd:PRK09274 174 DDMAAILFTSGSTGTPKGVVYT-HGMFEAQIEALREDYGIEPGEIDLPTFPLF---AL-----FGPALGMTSVIpdMdPT 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 247 RPV---PASVYETIARYRPTLYFGVPTL------YGsmLQEEGSLDGVRLCVSAGEALPADIFKRWQQ--RHGVEIIDGI 315
Cdd:PRK09274 245 RPAtvdPAKLFAAIERYGVTNLFGSPALlerlgrYG--EANGIKLPSLRRVISAGAPVPIAVIERFRAmlPPDAEILTPY 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 316 GSTE---LSHIFISNLPGKAKPGSS-------GKVVPGYDARIVD------ENFQDI---PVGEVGTLLIKGDSAAAAYW 376
Cdd:PRK09274 323 GATEalpISSIESREILFATRAATDngagicvGRPVDGVEVRIIAisdapiPEWDDAlrlATGEIGEIVVAGPMVTRSYY 402
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 700405679 377 NKHQKTKSTMLGD-----WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVG 444
Cdd:PRK09274 403 NRPEATRLAKIPDgqgdvWHRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVG 475
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
168-504 |
2.12e-23 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 101.69 E-value: 2.12e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 168 TPDDAcFWLYSSGSTGQPKGTIHLQHD-----MVYAAETYGKQVLGIQEHDICFSAAKL-FFA-----YGLGNGLYFPFS 236
Cdd:cd05924 2 SADDL-YILYTGGTTGMPKGVMWRQEDifrmlMGGADFGTGEFTPSEDAHKAAAAAAGTvMFPapplmHGTGSWTAFGGL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 237 VGATSVLMP-VRPVPASVYETIARYRPTLYFGVPTLYGSMLQEEGS------LDGVRLCVSAGEALPADIFKRWQQ-RHG 308
Cdd:cd05924 81 LGGQTVVLPdDRFDPEEVWRTIEKHKVTSMTIVGDAMARPLIDALRdagpydLSSLFAISSGGALLSPEVKQGLLElVPN 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 309 VEIIDGIGSTELSHIFISNLPGKAkPGSSGKVVPGYDARIVDENFQDIPVGE-VGTLLIKGDSAAAAYWNKHQKTKSTM- 386
Cdd:cd05924 161 ITLVDAFGSSETGFTGSGHSAGSG-PETGPFTRANPDTVVLDDDGRVVPPGSgGVGWIARRGHIPLGYYGDEAKTAETFp 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 -LGD--WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRG 463
Cdd:cd05924 240 eVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVVQLREG 319
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 700405679 464 YAPDDQlkkDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:cd05924 320 AGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
29-510 |
3.48e-23 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 101.86 E-value: 3.48e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 29 KTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLND 108
Cdd:cd17645 14 HVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGERIAYMLAD 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 109 SRAKILIIDeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKGT 188
Cdd:cd17645 94 SSAKILLTN---------------------------------------------------PDDLAYVIYTSGSTGLPKGV 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 189 IHLQHDMVYAAETYgKQVLGIQEHDicfsAAKLFFAYGLGNGLY--FPF-SVGATsvlmpVRPVPASV---YETIARYRP 262
Cdd:cd17645 123 MIEHHNLVNLCEWH-RPYFGVTPAD----KSLVYASFSFDASAWeiFPHlTAGAA-----LHVVPSERrldLDALNDYFN 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 263 TLYFGVPTLYGSMLQEEGSLD--GVRLCVSAGealpaDIFKRWQqRHGVEIIDGIGSTELShIFISNLPGKAKPGS--SG 338
Cdd:cd17645 193 QEGITISFLPTGAAEQFMQLDnqSLRVLLTGG-----DKLKKIE-RKGYKLVNNYGPTENT-VVATSFEIDKPYANipIG 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 339 KVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-------WVNTDDKYAVDEEGYFYYIGRS 411
Cdd:cd17645 266 KPIDNTRVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHpfvpgerMYRTGDLAKFLPDGNIEFLGRL 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 412 NDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATD---DKNLVkpkAFVVLKRGYAPDdqlkkDLKEYVKKTLAHYKYP 488
Cdd:cd17645 346 DQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDadgRKYLV---AYVTAPEEIPHE-----ELREWLKNDLPDYMIP 417
|
490 500
....*....|....*....|..
gi 700405679 489 RWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd17645 418 TYFVHLKALPLTANGKVDRKAL 439
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
37-445 |
4.99e-23 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 102.50 E-value: 4.99e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 37 NAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILII 116
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 -DEELYSKFEPIKDKLRFLKHIIVVNgqaPAGT--------ISFDSFVA----------GASDQLEAApITPDDACFWLY 177
Cdd:cd17641 90 eDEEQVDKLLEIADRIPSVRYVIYCD---PRGMrkyddprlISFEDVVAlgraldrrdpGLYEREVAA-GKGEDVAVLCT 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLF----FAYGLGNGLY------FPFS----------V 237
Cdd:cd17641 166 TSGTTGKPKLAM-LSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPwigeQMYSVGQALVcgfivnFPEEpetmmedlreI 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 238 GATSVLMPVR-----------------PVPASVYE-----------TIARYRPTL-------YFGVPTLYGsMLQEEGSL 282
Cdd:cd17641 245 GPTFVLLPPRvwegiaadvrarmmdatPFKRFMFElgmklglraldRGKRGRPVSlwlrlasWLADALLFR-PLRDRLGF 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 283 DGVRLCVSAGEALPADIFkRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVdenfqdipvgEVG 362
Cdd:cd17641 324 SRLRSAATGGAALGPDTF-RFFHAIGVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVPFPGTEVRID----------EVG 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 363 TLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVG-GIWVSPVEVEACLIEHPAVLEC 440
Cdd:cd17641 393 EILVRSPGVFVGYYKNPEATAEDFDEDgWLHTGDAGYFKENGHLVVIDRAKDVGTTSdGTRFSPQFIENKLKFSPYIAEA 472
|
....*
gi 700405679 441 AVVGA 445
Cdd:cd17641 473 VVLGA 477
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
38-512 |
1.83e-22 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 100.43 E-value: 1.83e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQpkdylYFLNDSRAK----- 112
Cdd:cd05906 39 FQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVPPT-----YDEPNARLRklrhi 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 113 -------ILIIDEELYSKFEPIKdKLRFLKHIIVvngqapagtISFDSFVAGASDqLEAAPITPDDACFWLYSSGSTGQP 185
Cdd:cd05906 114 wqllgspVVLTDAELVAEFAGLE-TLSGLPGIRV---------LSIEELLDTAAD-HDLPQSRPDDLALLMLTSGSTGFP 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 186 KGTIHLQHDMVYAAEtyGK-QVLGIQEHDICFSaaklffayglgnglYFPFSVGATSVLMPVRPV--------------- 249
Cdd:cd05906 183 KAVPLTHRNILARSA--GKiQHNGLTPQDVFLN--------------WVPLDHVGGLVELHLRAVylgcqqvhvpteeil 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 250 --PASVYETIARYRPTLYFGVPTLYGSMLQ--EE-----GSLDGVRLCVSAGEALPADIFKRWQQ---RHGVE---IIDG 314
Cdd:cd05906 247 adPLRWLDLIDRYRVTITWAPNFAFALLNDllEEiedgtWDLSSLRYLVNAGEAVVAKTIRRLLRllePYGLPpdaIRPA 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 315 IGSTEL-SHIFISNLPGKAKPG------SSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML 387
Cdd:cd05906 327 FGMTETcSGVIYSRSFPTYDHSqalefvSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFT 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 388 GD-WVNTDDKYAVDeEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAV-----LECAVVGATDDKNLVkpkAFVVLK 461
Cdd:cd05906 407 EDgWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVepsftAAFAVRDPGAETEEL---AIFFVP 482
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 462 RgYAPDDQLKKDLKEyVKKTLAHyKYPRWIEFV-----DDLPKTATGKLKRFELRA 512
Cdd:cd05906 483 E-YDLQDALSETLRA-IRSVVSR-EVGVSPAYLiplpkEEIPKTSLGKIQRSKLKA 535
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
37-429 |
1.47e-21 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 98.88 E-value: 1.47e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 37 NAFTYAQVQAGMNKVGNALKGLGIEMENrVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDylyFLNDSRA---KI 113
Cdd:PRK06814 657 GPLTYRKLLTGAFVLGRKLKKNTPPGEN-VGVMLPNANGAAVTFFALQSAGRVPAMINFSAGIAN---ILSACKAaqvKT 732
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 114 LIIDEELYSK--FEPIKDKLRFLKHII----VVNGQAPAGTIsfDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PRK06814 733 VLTSRAFIEKarLGPLIEALEFGIRIIyledVRAQIGLADKI--KGLLAGRFPLVYFCNRDPDDPAVILFTSGSEGTPKG 810
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIhLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMP----VRPVPASVYETIAryrpT 263
Cdd:PRK06814 811 VV-LSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFGLTGGLVLPLLSGVKVFLYPsplhYRIIPELIYDTNA----T 885
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQEEGSLD--GVRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVV 341
Cdd:PRK06814 886 ILFGTDTFLNGYARYAHPYDfrSLRYVFAGAEKVKEETRQTWMEKFGIRILEGYGVTETAPVIALNTPMHNKAGTVGRLL 965
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 342 PGYDARIvdENFQDIPVGevGTLLIKGDSAAAAYWnkhQKTKSTML----GDWVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK06814 966 PGIEYRL--EPVPGIDEG--GRLFVRGPNVMLGYL---RAENPGVLeppaDGWYDTGDIVTIDEEGFITIKGRAKRFAKI 1038
|
410
....*....|..
gi 700405679 418 GGIWVSPVEVEA 429
Cdd:PRK06814 1039 AGEMISLAAVEE 1050
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
36-511 |
2.19e-21 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 96.73 E-value: 2.19e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 36 DNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILI 115
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDeelyskfepIKDKLRflkhiivvngqapagtisfdsfvAGASDQLEAAPITP-DDACFWLYSSGSTGQPKGTI--HLQ 192
Cdd:cd05939 81 FN---------LLDPLL-----------------------TQSSTEPPSQDDVNfRDKLFYIYTSGTTGLPKAAVivHSR 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 193 HDMVYAAETYGkqvLGIQEHDICFSAAKLFFA----YGLGNGLYFpfsvGATSVLMpvRPVPASVY-ETIARYRPTL--Y 265
Cdd:cd05939 129 YYRIAAGAYYA---FGMRPEDVVYDCLPLYHSaggiMGVGQALLH----GSTVVIR--KKFSASNFwDDCVKYNCTIvqY 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 266 FGVPTLY--GSMLQEEGSLDGVRLCVsaGEALPADIFKRWQQRHGVEIIDGI-GSTE----LSHI--------FISNLPG 330
Cdd:cd05939 200 IGEICRYllAQPPSEEEQKHNVRLAV--GNGLRPQIWEQFVRRFGIPQIGEFyGATEgnssLVNIdnhvgacgFNSRILP 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 331 KAKPGSSGKVVPGYDARIVDENFQDIPV--GEVGTL---LIKGD--SAAAAYWNK---HQKTKSTML--GDWV-NTDDKY 397
Cdd:cd05939 278 SVYPIRLIKVDEDTGELIRDSDGLCIPCqpGEPGLLvgkIIQNDplRRFDGYVNEgatNKKIARDVFkkGDSAfLSGDVL 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 398 AVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKP--KAFVVLKRGYAPdDQLKKDLk 475
Cdd:cd05939 358 VMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVEVPGVEGRAgmAAIVDPERKVDL-DRFSAVL- 435
|
490 500 510
....*....|....*....|....*....|....*.
gi 700405679 476 eyvKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05939 436 ---AKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQ 468
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
40-428 |
4.27e-21 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 96.66 E-value: 4.27e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID-E 118
Cdd:cd05933 10 TYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVVEnQ 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 119 ELYSKFEPIKDKLRFLKHIIVVNG---QAPAGTISFDSFVAGASD----QLEA--APITPDDACFWLYSSGSTGQPKGtI 189
Cdd:cd05933 90 KQLQKILQIQDKLPHLKAIIQYKEplkEKEPNLYSWDEFMELGRSipdeQLDAiiSSQKPNQCCTLIYTSGTTGMPKG-V 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 HLQHDMVYAAETYGKQVLGIQEHDI-----------CFSAAKLFfayglgnGLYFPFSVGATSVLMPVRPVPASVYETIA 258
Cdd:cd05933 169 MLSHDNITWTAKAASQHMDLRPATVgqesvvsylplSHIAAQIL-------DIWLPIKVGGQVYFAQPDALKGTLVKTLR 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 259 RYRPTLYFGVPTLY----------------------------------GSMLQEEGS--------------------LDG 284
Cdd:cd05933 242 EVRPTAFMGVPRVWekiqekmkavgaksgtlkrkiaswakgvgletnlKLMGGESPSplfyrlakklvfkkvrkalgLDR 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 285 VRLCVSAGEALPADIFKRWQQRHgVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVDENFQDIpvgevGTL 364
Cdd:cd05933 322 CQKFFTGAAPISRETLEFFLSLN-IPIMELYGMSETSGPHTISNPQAYRLLSCGKALPGCKTKIHNPDADGI-----GEI 395
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 365 LIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKV-GGIWVSPVEVE 428
Cdd:cd05933 396 CFWGRHVFMGYLNMEDKTEEAIDEDgWLHSGDLGKLDEDGFLYITGRIKELIITaGGENVPPVPIE 461
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
21-512 |
6.65e-21 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 95.84 E-value: 6.65e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 21 NIREGRGQktaiLCRdnAFTYAQVQAGMNKVGNALKGLGIEMENRVALLL-LDSEFYPLsFFGAIKIGAVPICL---NTL 96
Cdd:PRK09192 38 NFYDRRGQ----LEE--ALPYQTLRARAEAGARRLLALGLKPGDRVALIAeTDGDFVEA-FFACQYAGLVPVPLplpMGF 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 97 MQPKDYLY----FLNDSRAKILIIDEELyskfepikdkLRFLKHIivVNGQAPAGTISFDSFVAGASDQLEAAPITPDDA 172
Cdd:PRK09192 111 GGRESYIAqlrgMLASAQPAAIITPDEL----------LPWVNEA--THGNPLLHVLSHAWFKALPEADVALPRPTPDDI 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 173 CFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMPVRPV--- 249
Cdd:PRK09192 179 AYLQYSSGSTRFPRGVIITHRALMANLRAISHDGLKVRPGDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLPTRDFarr 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 250 PASVYETIARYRPTLYFGvPTlYGSML-------QEEGSLD--GVRLCVSAGEALPADIFKRWQQRHG------------ 308
Cdd:PRK09192 259 PLQWLDLISRNRGTISYS-PP-FGYELcarrvnsKDLAELDlsCWRVAGIGADMIRPDVLHQFAEAFApagfddkafmps 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 309 --------------------VEIIDgigSTELSHIFISnLPGKAKPGSS------GKVVPGYDARIVDENFQDIPVGEVG 362
Cdd:PRK09192 337 yglaeatlavsfsplgsgivVEEVD---RDRLEYQGKA-VAPGAETRRVrtfvncGKALPGHEIEIRNEAGMPLPERVVG 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 363 TLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDD-KYAVDeeGYFYYIGRSNDMLKVGG--IWvsPVEVEACLIEHPAVLE 439
Cdd:PRK09192 413 HICVRGPSLMSGYFRDEESQDVLAADGWLDTGDlGYLLD--GYLYITGRAKDLIIINGrnIW--PQDIEWIAEQEPELRS 488
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 440 C-AVVGATDDKNLVKPkAFVVLKRGYAPDD--QLKKDLKEYVKktlAHYKYPRWIEFV--DDLPKTATGKLKRFELRA 512
Cdd:PRK09192 489 GdAAAFSIAQENGEKI-VLLVQCRISDEERrgQLIHALAALVR---SEFGVEAAVELVppHSLPRTSSGKLSRAKAKK 562
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
38-507 |
1.01e-20 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 95.34 E-value: 1.01e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:PRK05852 43 AISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVVLID 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 EELYSKFEPikDKLRFLKHIIVVNG--QAPAGTISFDSFVAGASDQLEAAP--ITPDDACFwLYSSGSTGQPKGTIHLQH 193
Cdd:PRK05852 123 ADGPHDRAE--PTTRWWPLTVNVGGdsGPSGGTLSVHLDAATEPTPATSTPegLRPDDAMI-MFTGGTTGLPKMVPWTHA 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 DMvyAAETYGK-QVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATsVLMPVRP--VPASVYETIARYRPTLYFGVPT 270
Cdd:PRK05852 200 NI--ASSVRAIiTGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGA-VLLPARGrfSAHTFWDDIKAVGATWYTAVPT 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 271 LYGSMLQ---EEGSLDG---VRLCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSH-IFISNLPGKAK---PGSSGKV 340
Cdd:PRK05852 277 IHQILLEraaTEPSGRKpaaLRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEATHqVTTTQIEGIGQtenPVVSTGL 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 341 VP---GYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKV 417
Cdd:PRK05852 357 VGrstGAQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINR 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 418 GGIWVSPVEVEACLIEHPAVLECAVVGATdDKNLVKPKAFVVLKRGYAPDDqlKKDLKEYVKKTLAHYKYPRWIEFVDDL 497
Cdd:PRK05852 437 GGEKISPERVEGVLASHPNVMEAAVFGVP-DQLYGEAVAAVIVPRESAPPT--AEELVQFCRERLAAFEIPASFQEASGL 513
|
490
....*....|
gi 700405679 498 PKTATGKLKR 507
Cdd:PRK05852 514 PHTAKGSLDR 523
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
40-409 |
3.15e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 93.96 E-value: 3.15e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIG--AVPICLNTLMQPKDY--LYFLNDSRAKILI 115
Cdd:PRK12582 82 TYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGvpAAPVSPAYSLMSHDHakLKHLFDLVKPRVV 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 116 IDEELySKFEPIKDKLRFLKHIIVVNGQAPAG--TISFDSFVAGA-SDQLEAA--PITPDDACFWLYSSGSTGQPKGTIH 190
Cdd:PRK12582 162 FAQSG-APFARALAALDLLDVTVVHVTGPGEGiaSIAFADLAATPpTAAVAAAiaAITPDTVAKYLFTSGSTGMPKAVIN 240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 lQHDMVYAAETYGKQV--------LGIQ------EHdiCFSAAKLFfayglgNGLYFPfsvGATSVLMPVRPVPASVYET 256
Cdd:PRK12582 241 -TQRMMCANIAMQEQLrprepdppPPVSldwmpwNH--TMGGNANF------NGLLWG---GGTLYIDDGKPLPGMFEET 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYR---PTLYFGVPTLYgSMLQEEGSLDGVrLCVS----------AGEALPADIFKRWQQR------HGVEIIDGIGS 317
Cdd:PRK12582 309 IRNLReisPTVYGNVPAGY-AMLAEAMEKDDA-LRRSffknlrlmayGGATLSDDLYERMQALavrttgHRIPFYTGYGA 386
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 318 TELSHIFISNLPGKAKPGSSGKVVPGYDARIVdenfqdiPVGEVGTLLIKGDSAAAAYWNKHQKTKStmlgdwvntddky 397
Cdd:PRK12582 387 TETAPTTTGTHWDTERVGLIGLPLPGVELKLA-------PVGDKYEVRVKGPNVTPGYHKDPELTAA------------- 446
|
410
....*....|..
gi 700405679 398 AVDEEGyFYYIG 409
Cdd:PRK12582 447 AFDEEG-FYRLG 457
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
28-507 |
3.18e-20 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 94.85 E-value: 3.18e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLN 107
Cdd:PRK05691 1146 ERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAERLAYMLA 1225
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 108 DSRAKILIIDEELYSKFEpikdklrflkhiivvngqAPAGTISfdsfVAGASDQLEAAPITP-------DDACFWLYSSG 180
Cdd:PRK05691 1226 DSGVELLLTQSHLLERLP------------------QAEGVSA----IALDSLHLDSWPSQApglhlhgDNLAYVIYTSG 1283
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 181 STGQPK--GTIH--LQHDMVYAAETYGkqvlgIQEHDICFSAAKLFFAYGLGNgLYFPFSVGATSVLMPV--RPVPASVY 254
Cdd:PRK05691 1284 STGQPKgvGNTHaaLAERLQWMQATYA-----LDDSDVLMQKAPISFDVSVWE-CFWPLITGCRLVLAGPgeHRDPQRIA 1357
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 255 ETIARYR-PTLYFgVPTLYGSMLQEEGSLD--GVRLCVSAGEALPADIFKR-WQQRHGVEIIDGIGSTE----LSHIFIS 326
Cdd:PRK05691 1358 ELVQQYGvTTLHF-VPPLLQLFIDEPLAAActSLRRLFSGGEALPAELRNRvLQRLPQVQLHNRYGPTEtainVTHWQCQ 1436
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 327 NLPGKAKPgsSGKVVPGYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTD--------DKYA 398
Cdd:PRK05691 1437 AEDGERSP--IGRPLGNVLCRVLDAELNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPLGEDgarlyrtgDRAR 1514
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 399 VDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDdknlVKPKAFVVLKRGYAPDDQLKKDLKEYV 478
Cdd:PRK05691 1515 WNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREG----AAGAQLVGYYTGEAGQEAEAERLKAAL 1590
|
490 500
....*....|....*....|....*....
gi 700405679 479 KKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK05691 1591 AAELPEYMVPAQLIRLDQMPLGPSGKLDR 1619
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
6-512 |
4.48e-20 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 93.15 E-value: 4.48e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 6 NLPETFnaadyfVDRNIREGRGQKTAILCR----DNAFTYAQVQAGMNKVGNALKGLGIEMENRVaLLLLDS--EFYpLS 79
Cdd:PRK05857 11 QLPSTV------LDRVFEQARQQPEAIALRrcdgTSALRYRELVAEVGGLAADLRAQSVSRGSRV-LVISDNgpETY-LS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 80 FFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNGqapAGTISFDSfvAGAS 159
Cdd:PRK05857 83 VLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKMASSAVPEALHSIPVIAVDIA---AVTRESEH--SLDA 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 160 DQLEAAP-ITPDDACFWLYSSGSTGQPKGTIhLQHDMVYAAEtygkqvlgiqehDICFSAAKLFFAYGLGNGLYFPFSvg 238
Cdd:PRK05857 158 ASLAGNAdQGSEDPLAMIFTSGTTGEPKAVL-LANRTFFAVP------------DILQKEGLNWVTWVVGETTYSPLP-- 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 239 ATSV---------LMPVRPV------PASVYETIARYRPTLYFGVPTLYGSMLQEEGSLDGV----RLCVSAG-EALPAD 298
Cdd:PRK05857 223 ATHIgglwwiltcLMHGGLCvtggenTTSLLEILTTNAVATTCLVPTLLSKLVSELKSANATvpslRLVGYGGsRAIAAD 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 299 IfkRWQQRHGVEIIDGIGSTELSHIFISnLP------GKAKPGSSGKVVPGYDARIVDENFQD------IPVGEVGTLLI 366
Cdd:PRK05857 303 V--RFIEATGVRTAQVYGLSETGCTALC-LPtddgsiVKIEAGAVGRPYPGVDVYLAATDGIGptapgaGPSASFGTLWI 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 367 KGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEV-----------EACLIEHP 435
Cdd:PRK05857 380 KSPANMLGYWNNPERTAEVLIDGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVdriaegvsgvrEAACYEIP 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 436 -----AVLECAVVGATDdknlVKPKAFVVLKRGYApddqlkkdlkeyvkktlAHYK-------YPRWIEFVDDLPKTATG 503
Cdd:PRK05857 460 deefgALVGLAVVASAE----LDESAARALKHTIA-----------------ARFRresepmaRPSTIVIVTDIPRTQSG 518
|
....*....
gi 700405679 504 KLKRFELRA 512
Cdd:PRK05857 519 KVMRASLAA 527
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
127-504 |
1.27e-18 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 89.25 E-value: 1.27e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 127 IKDKLRFLKHIIVVNGQAPAGTI---------SFDSFVAGASD-QLEAAPITPDDACFWLYSSGSTGQPKGTIH------ 190
Cdd:cd05943 196 LVKGLPSLLAVVVVPYTVAAGQPdlskiakalTLEDFLATGAAgELEFEPLPFDHPLYILYSSGTTGLPKCIVHgaggtl 275
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 LQHdmvyaaetygkqvlgIQEHDICF---SAAKLFFAYGLG----NGLYFPFSVGATSVLM---PVRPVPASVYETIARY 260
Cdd:cd05943 276 LQH---------------LKEHILHCdlrPGDRLFYYTTCGwmmwNWLVSGLAVGATIVLYdgsPFYPDTNALWDLADEE 340
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 261 RPTlYFGVPTLYGSMLQEEG-------SLDGVRLCVSAGEALPADIFkRWQQRH---GVEIIDGIGSTELSHIFI---SN 327
Cdd:cd05943 341 GIT-VFGTSAKYLDALEKAGlkpaethDLSSLRTILSTGSPLKPESF-DYVYDHikpDVLLASISGGTDIISCFVggnPL 418
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 328 LPgkAKPGSSGKVVPGYDARIVDENFQDIpVGEVGTLLIK-----------GDSAA----AAYWNK------Hqktkstm 386
Cdd:cd05943 419 LP--VYRGEIQCRGLGMAVEAFDEEGKPV-WGEKGELVCTkpfpsmpvgfwNDPDGsryrAAYFAKypgvwaH------- 488
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 lGDWVNtddkyaVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAP 466
Cdd:cd05943 489 -GDWIE------ITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVEL 561
|
410 420 430
....*....|....*....|....*....|....*...
gi 700405679 467 DDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGK 504
Cdd:cd05943 562 DDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGK 599
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
38-511 |
1.66e-18 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 87.87 E-value: 1.66e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 38 AFTYAQVQAGMNKVGNALKG-LGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILII 116
Cdd:cd05937 5 TWTYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFVIV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 117 DeelyskfepikdklrflkhiivvngqapagtisfdsfvagasdqleaapitPDDACFWLYSSGSTGQPKGTIhLQHDMV 196
Cdd:cd05937 85 D---------------------------------------------------PDDPAILIYTSGTTGLPKAAA-ISWRRT 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 197 YAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMP---VRPVPASVYET----------IARYrpt 263
Cdd:cd05937 113 LVTSNLLSHDLNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALSRkfsASQFWKDVRDSgatiiqyvgeLCRY--- 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 264 LYFGVPTLYGSMLQeegsldgVRlcVSAGEALPADIFKRWQQRHGVEII-------DGIGSTElshIFISNLPGKAKPGS 336
Cdd:cd05937 190 LLSTPPSPYDRDHK-------VR--VAWGNGLRPDIWERFRERFNVPEIgefyaatEGVFALT---NHNVGDFGAGAIGH 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 337 SGKVVP---GYDARIV--DENFQDI------------PVGEVGTLLI----KGDSAAAAYWNKHQKTKSTMLGD------ 389
Cdd:cd05937 258 HGLIRRwkfENQVVLVkmDPETDDPirdpktgfcvraPVGEPGEMLGrvpfKNREAFQGYLHNEDATESKLVRDvfrkgd 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 390 -WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPK-AFVVLKRGYA-P 466
Cdd:cd05937 338 iYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVYGVKVPGHDGRAGcAAITLEESSAvP 417
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 700405679 467 DDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFELR 511
Cdd:cd05937 418 TEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLR 462
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
163-507 |
2.48e-18 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 88.26 E-value: 2.48e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 163 EAAPITPDDACFWLYSSGSTGQPKGTIhlqhdmvyaaETYGKQVLGIQEHDICFSAAK----LFFAYGLG-----NGLYF 233
Cdd:PTZ00237 247 EYVPVESSHPLYILYTSGTTGNSKAVV----------RSNGPHLVGLKYYWRSIIEKDiptvVFSHSSIGwvsfhGFLYG 316
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 234 PFSVGATSVL-----MPVRPVPASVYETIARYRPTLYFGVPTLY---------GSMLQEEGSLDGVRLCVSAGEALPADI 299
Cdd:PTZ00237 317 SLSLGNTFVMfeggiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIryliktdpeATIIRSKYDLSNLKEIWCGGEVIEESI 396
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 300 FKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKP-GSSGKVVPGYDARIVDENFQDIPVGEVGTLLIK---GDSAAAAY 375
Cdd:PTZ00237 397 PEYIENKLKIKSSRGYGQTEIGITYLYCYGHINIPyNATGVPSIFIKPSILSEDGKELNVNEIGEVAFKlpmPPSFATTF 476
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 376 WNKHQKTKS--TMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVK 453
Cdd:PTZ00237 477 YKNDEKFKQlfSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNV 556
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 454 PKAFVVLKRGYAPD----DQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PTZ00237 557 PIGLLVLKQDQSNQsidlNKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
170-446 |
1.82e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 84.82 E-value: 1.82e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 170 DDACFWLYSSGSTGQPKGTIHlQHDMVYAAETYGKQVLGIQEHDICFSAAKLFfayglgnGLYFPfSVGATSVLMPVRPV 249
Cdd:cd05910 85 DEPAAILFTSGSTGTPKGVVY-RHGTFAAQIDALRQLYGIRPGEVDLATFPLF-------ALFGP-ALGLTSVIPDMDPT 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 250 ------PASVYETIARYRPTLYFGVPTL------YGsmLQEEGSLDGVRLCVSAGEALPADIFKRWQQ--RHGVEIIDGI 315
Cdd:cd05910 156 rparadPQKLVGAIRQYGVSIVFGSPALlervarYC--AQHGITLPSLRRVLSAGAPVPIALAARLRKmlSDEAEILTPY 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 316 GSTEL-------SHIFISNLPGKAKPGSS---GKVVPGYDARIVDENFQDI---------PVGEVGTLLIKGDSAAAAYW 376
Cdd:cd05910 234 GATEAlpvssigSRELLATTTAATSGGAGtcvGRPIPGVRVRIIEIDDEPIaewddtlelPRGEIGEITVTGPTVTPTYV 313
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 700405679 377 NKHQKTKSTMLGD-----WVNTDDKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGAT 446
Cdd:cd05910 314 NRPVATALAKIDDnsegfWHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALVGVG 388
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
137-432 |
6.59e-16 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 80.69 E-value: 6.59e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 137 IIVVNGQAPAGTISFDSFVA---GASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHlQHDMVYAAETYGKQVLGiqehd 213
Cdd:PRK08180 173 VAVRGAVPGRAATPFAALLAtppTAAVDAAHAAVGPDTIAKFLFTSGSTGLPKAVIN-THRMLCANQQMLAQTFP----- 246
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 214 icfsaaklFFAYG-------------------LGNGLYFpfsvGATSVLMPVRPVPASVYETIARYR---PTLYFGVPTL 271
Cdd:PRK08180 247 --------FLAEEppvlvdwlpwnhtfggnhnLGIVLYN----GGTLYIDDGKPTPGGFDETLRNLReisPTVYFNVPKG 314
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 272 YGSMLQE--------EGSLDGVRLCVSAGEALPADIFKRWQ---QRH-G--VEIIDGIGSTELSHIFISNLPGKAKPGSS 337
Cdd:PRK08180 315 WEMLVPAlerdaalrRRFFSRLKLLFYAGAALSQDVWDRLDrvaEATcGerIRMMTGLGMTETAPSATFTTGPLSRAGNI 394
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVdenfqdiPVGEVGTLLIKGDSAAAAYWNKHQKTKStmlgdwvntddkyAVDEEGYFyyigRSNDMLKv 417
Cdd:PRK08180 395 GLPAPGCEVKLV-------PVGGKLEVRVKGPNVTPGYWRAPELTAE-------------AFDEEGYY----RSGDAVR- 449
|
330
....*....|....*
gi 700405679 418 ggiWVSPVEVEACLI 432
Cdd:PRK08180 450 ---FVDPADPERGLM 461
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
28-507 |
7.19e-16 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 79.94 E-value: 7.19e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 28 QKTAILCRDNAFTYAQVQAGMNKVGNALKGLGIEMENRVaLLLLDSEFYPL-SFFGAIKIGA--VPICLNTlmqPKDYLY 104
Cdd:PRK04813 17 DFPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPI-IVFGHMSPEMLaTFLGAVKAGHayIPVDVSS---PAERIE 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 105 FLND-SRAKILIIDEELYSKFEPIKdklrFLKHIIVVNGQAPAGTISFDSFVAGasdqleaapitpDDACFWLYSSGSTG 183
Cdd:PRK04813 93 MIIEvAKPSLIIATEELPLEILGIP----VITLDELKDIFATGNPYDFDHAVKG------------DDNYYIIFTSGTTG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 184 QPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAklffayglgnglyfPFS-------------VGATSVLMP--VRP 248
Cdd:PRK04813 157 KPKG-VQISHDNLVSFTNWMLEDFALPEGPQFLNQA--------------PYSfdlsvmdlyptlaSGGTLVALPkdMTA 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPASVYETIaryrPTLYFGV--------------PTLYGSMLqeeGSLDGVRLCvsaGEALPAD----IFKRWQQRHgve 310
Cdd:PRK04813 222 NFKQLFETL----PQLPINVwvstpsfadmclldPSFNEEHL---PNLTHFLFC---GEELPHKtakkLLERFPSAT--- 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 311 IIDGIGSTE----LSHIFISN--------LP-GKAKPGSsgkvvpgyDARIVDENFQDIPVGEVGTLLIKGDSAAAAYWN 377
Cdd:PRK04813 289 IYNTYGPTEatvaVTSIEITDemldqykrLPiGYAKPDS--------PLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLN 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 378 KHQKTKSTMlgdwvNTDDKY--------AVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD- 448
Cdd:PRK04813 361 NPEKTAEAF-----FTFDGQpayhtgdaGYLEDGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPYNKDh 435
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 700405679 449 --KNLVkpkAFVVLKRG-YAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKR 507
Cdd:PRK04813 436 kvQYLI---AYVVPKEEdFEREFELTKAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDR 494
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
169-434 |
1.67e-15 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 79.09 E-value: 1.67e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGaTSVLMPVRP 248
Cdd:PRK06334 182 PEDVAVILFTSGTEKLPKG-VPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSG-VPVVFAYNP 259
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 V-PASVYETIARYRPTLYFGVPTLYGSML----QEEGSLDGVRLCVSAGEALPADIFKRWQ--QRHgVEIIDGIGSTELS 321
Cdd:PRK06334 260 LyPKKIVEMIDEAKVTFLGSTPVFFDYILktakKQESCLPSLRFVVIGGDAFKDSLYQEALktFPH-IQLRQGYGTTECS 338
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 322 H-IFISNLPGKAKPGSSGKVVPGYDARIV-DENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLG--DWVNTDDKY 397
Cdd:PRK06334 339 PvITINTVNSPKHESCVGMPIRGMDVLIVsEETKVPVSSGETGLVLTRGTSLFSGYLGEDFGQGFVELGgeTWYVTGDLG 418
|
250 260 270
....*....|....*....|....*....|....*..
gi 700405679 398 AVDEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEH 434
Cdd:PRK06334 419 YVDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEG 455
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
123-505 |
7.91e-15 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 77.14 E-value: 7.91e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 123 KFEPIKDKLRFLKHIIVVN-------GQAPAGTISFDSFVAGASD-QLEAAPITPDDACFWLYSSGSTGQPKGTIH---- 190
Cdd:PRK03584 208 KVAELRAALPSLEHVVVVPylgpaaaAAALPGALLWEDFLAPAEAaELEFEPVPFDHPLWILYSSGTTGLPKCIVHghgg 287
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 191 --LQHdmvyaaetygkqvlgIQEH----DIcfSAAKLFFAY-------------GLGnglyfpfsVGATSVLM---PVRP 248
Cdd:PRK03584 288 ilLEH---------------LKELglhcDL--GPGDRFFWYttcgwmmwnwlvsGLL--------VGATLVLYdgsPFYP 342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 249 VPASVYETIARYRPTlYFGVPTLYGSMLQ-------EEGSLDGVRLCVSAGEALPADIFkRWQQRH---GVEIIDGIGST 318
Cdd:PRK03584 343 DPNVLWDLAAEEGVT-VFGTSAKYLDACEkaglvpgETHDLSALRTIGSTGSPLPPEGF-DWVYEHvkaDVWLASISGGT 420
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 319 ELSHIFI---SNLPgkakpgssgkVVPG--------YDARIVDENFQDIpVGEVGTLLIK-----------GDSAA---- 372
Cdd:PRK03584 421 DICSCFVggnPLLP----------VYRGeiqcrglgMAVEAWDEDGRPV-VGEVGELVCTkpfpsmplgfwNDPDGsryr 489
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 373 AAYWNK------HqktkstmlGDWVntddkyAVDEEGYFYYIGRSNDMLKVGGIWVSPVE----VEACliehPAVLECAV 442
Cdd:PRK03584 490 DAYFDTfpgvwrH--------GDWI------EITEHGGVVIYGRSDATLNRGGVRIGTAEiyrqVEAL----PEVLDSLV 551
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 700405679 443 VGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATGKL 505
Cdd:PRK03584 552 IGQEWPDGDVRMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKK 614
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
139-512 |
8.78e-15 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 75.85 E-value: 8.78e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 139 VVNGQAPAgtisfdsFVA-GASDQLEAA----------PITPDDACFwLYSSGSTGQPKGTIHLQHDMVYAAE-TY---- 202
Cdd:PRK07824 1 ALAGRAPA-------LLPvPAQDERRAAllrdalrvgePIDDDVALV-VATSGTTGTPKGAMLTAAALTASADaTHdrlg 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 203 --GKQVLGIQEHDICfsaaklffayglgnGLyfpfSVGATSVLMPVRPV---------PASVYETIARYR-PTLYFG-VP 269
Cdd:PRK07824 73 gpGQWLLALPAHHIA--------------GL----QVLVRSVIAGSEPVeldvsagfdPTALPRAVAELGgGRRYTSlVP 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 270 T-LYGSMLQEEG-----SLDGVRLcvsAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIFISNlpgkakpgssGKVVPG 343
Cdd:PRK07824 135 MqLAKALDDPAAtaalaELDAVLV---GGGPAPAPVLDAAAAA-GINVVRTYGMSETSGGCVYD----------GVPLDG 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 344 YDARIVDenfqdipvgevGTLLIKGDSAAAAYWNKHQKTKSTMLGdWVNTDDKYAVDEeGYFYYIGRSNDMLKVGGIWVS 423
Cdd:PRK07824 201 VRVRVED-----------GRIALGGPTLAKGYRNPVDPDPFAEPG-WFRTDDLGALDD-GVLTVLGRADDAISTGGLTVL 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 424 PVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPddqLKKDLKEYVKKTLAHYKYPRWIEFVDDLPKTATG 503
Cdd:PRK07824 268 PQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPAP---TLEALRAHVARTLDRTAAPRELHVVDELPRRGIG 344
|
....*....
gi 700405679 504 KLKRFELRA 512
Cdd:PRK07824 345 KVDRRALVR 353
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
168-510 |
1.34e-14 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 75.97 E-value: 1.34e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 168 TPDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNgLYFPFSVGATSVLMP-- 245
Cdd:cd17654 116 TDECLAYVIHTSGTTGTPKI-VAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVE-IFLSLSSGATLLIVPts 193
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 VRPVPASVYETIA-RYRPTLYFGVPTL---YGSMLQEEGSLDG---VRLCVSAGEALPAD-IFKRWQQR-HGVEIIDGIG 316
Cdd:cd17654 194 VKVLPSKLADILFkRHRITVLQATPTLfrrFGSQSIKSTVLSAtssLRVLALGGEPFPSLvILSSWRGKgNRTRIFNIYG 273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 317 STELSHIFISN-LPGKAKPGSSGKVVPGYDARIVDENFQDIPvgevGTLLIKGDSAAAAYWNKHQKTKSTM--LGDWVNT 393
Cdd:cd17654 274 ITEVSCWALAYkVPEEDSPVQLGSPLLGTVIEVRDQNGSEGT----GQVFLGGLNRVCILDDEVTVPKGTMraTGDFVTV 349
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 394 ddkyavdEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVgATDDKNLVkpkAFVVlkrgyapdDQLKKD 473
Cdd:cd17654 350 -------KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGVESCAVT-LSDQQRLI---AFIV--------GESSSS 410
|
330 340 350
....*....|....*....|....*....|....*....
gi 700405679 474 L--KEYVKKTLAHYKYPRWIEFVDDLPKTATGKLKRFEL 510
Cdd:cd17654 411 RihKELQLTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
23-409 |
1.55e-14 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 75.93 E-value: 1.55e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 23 REGRGQKTAIlcrdnafTYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGaVPIC-----LNTLM 97
Cdd:cd05921 17 REGNGGWRRV-------TYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAG-VPAApvspaYSLMS 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 98 QPKDYLYFLNDSRAKILIIDEELyskfEPIKDKLR--FLKH--IIVVNGQAPA-GTISFDSFVA----GASDQLEAApIT 168
Cdd:cd05921 89 QDLAKLKHLFELLKPGLVFAQDA----APFARALAaiFPLGtpLVVSRNAVAGrGAISFAELAAtpptAAVDAAFAA-VG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHdMVYAAETYGKQVLGIQEHDICFSAAKLFFAYGLGNGLYFPFSV--GATSVLMPV 246
Cdd:cd05921 164 PDTVAKFLFTSGSTGLPKAVINTQR-MLCANQAMLEQTYPFFGEEPPVLVDWLPWNHTFGGNHNFNLVLynGGTLYIDDG 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 247 RPVPASVYETIARYR---PTLYFGVP----TLYGSMLQEEG----SLDGVRLCVSAGEALPADIFKRWQQR------HGV 309
Cdd:cd05921 243 KPMPGGFEETLRNLReisPTVYFNVPagweMLVAALEKDEAlrrrFFKRLKLMFYAGAGLSQDVWDRLQALavatvgERI 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 310 EIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVdenfqdiPVGEVGTLLIKGDSAAAAYWNKHQKTKStmlgd 389
Cdd:cd05921 323 PMMAGLGATETAPTATFTHWPTERSGLIGLPAPGTELKLV-------PSGGKYEVRVKGPNVTPGYWRQPELTAQ----- 390
|
410 420
....*....|....*....|
gi 700405679 390 wvntddkyAVDEEGyFYYIG 409
Cdd:cd05921 391 --------AFDEEG-FYCLG 401
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
169-507 |
6.17e-14 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 74.17 E-value: 6.17e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQV---LGIQEHDIC---------FSAAKLFFAYGLGNGLYFPFS 236
Cdd:cd17639 87 PDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRVpelLGPDDRYLAylplahifeLAAENVCLYRGGTIGYGSPRT 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 237 VGATSV------LMPVRP-----VPAsVYETIAR-----------YRPTLY--------FGVPTLYGSMLQEE------G 280
Cdd:cd17639 167 LTDKSKrgckgdLTEFKPtlmvgVPA-IWDTIRKgvlaklnpmggLKRTLFwtayqsklKALKEGPGTPLLDElvfkkvR 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 281 SLDG--VRLCVSAGEALPADIfKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVD------EN 352
Cdd:cd17639 246 AALGgrLRYMLSGGAPLSADT-QEFLNIVLCPVIQGYGLTETCAGGTVQDPGDLETGRVGPPLPCCEIKLVDweeggyST 324
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 353 FQDIPVGEVgtlLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKV-GGIWVSPVEVEAC 430
Cdd:cd17639 325 DKPPPRGEI---LIRGPNVFKGYYKNPEKTKEAFDGDgWFHTGDIGEFHPDGTLKIIDRKKDLVKLqNGEYIALEKLESI 401
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 431 LIEHPAVLE-CAVVgatdDKNLVKPKAFVV------------LKRGYAPDDQL--KKDLKEYVKKTLAH---------YK 486
Cdd:cd17639 402 YRSNPLVNNiCVYA----DPDKSYPVAIVVpnekhltklaekHGVINSEWEELceDKKLQKAVLKSLAEtaraaglekFE 477
|
410 420
....*....|....*....|....*..
gi 700405679 487 YPRWIEFVDDL--PK----TATGKLKR 507
Cdd:cd17639 478 IPQGVVLLDEEwtPEnglvTAAQKLKR 504
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
64-507 |
6.40e-14 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 75.20 E-value: 6.40e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 64 NRVALLLLDSEFYPLSFFGAIKIG--AVPICLNTLMQP---KDYLYFLNDSRAKILIIDEELyskfepiKDKLRFLKHII 138
Cdd:PRK05691 65 DRAVLLFPSGPDYVAAFFGCLYAGviAVPAYPPESARRhhqERLLSIIADAEPRLLLTVADL-------RDSLLQMEELA 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 139 VVNgqAPaGTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQHDMVYAAETYGKQVLGIQEH--DICF 216
Cdd:PRK05691 138 AAN--AP-ELLCVDTLDPALAEAWQEPALQPDDIAFLQYTSGSTALPKG-VQVSHGNLVANEQLIRHGFGIDLNpdDVIV 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 217 SAAKLFFAYGLGNGLYFPFSVGATSVLM--------PVRPVpasvyETIARYRPTLYFGvPTLYGSMLQE---EGSLDGV 285
Cdd:PRK05691 214 SWLPLYHDMGLIGGLLQPIFSGVPCVLMspayflerPLRWL-----EAISEYGGTISGG-PDFAYRLCSErvsESALERL 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 286 -----RLCVSAGEALPADIFKRWQQRH------------------------GVEIIDGIGSTEL-SHIFISNlpgKAKPG 335
Cdd:PRK05691 288 dlsrwRVAYSGSEPIRQDSLERFAEKFaacgfdpdsffasyglaeatlfvsGGRRGQGIPALELdAEALARN---RAEPG 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 336 ------SSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKST---MLGD-WVNTDDkYAVDEEGY 404
Cdd:PRK05691 365 tgsvlmSCGRSQPGHAVLIVDpQSLEVLGDNRVGEIWASGPSIAHGYWRNPEASAKTfveHDGRtWLRTGD-LGFLRDGE 443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 405 FYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVG--ATDDKNLVKPKAFVVLKRGYA---PDDQLKKDLKEYVK 479
Cdd:PRK05691 444 LFVTGRLKDMLIVRGHNLYPQDIEKTVEREVEVVRKGRVAafAVNHQGEEGIGIAAEISRSVQkilPPQALIKSIRQAVA 523
|
490 500 510
....*....|....*....|....*....|
gi 700405679 480 KtlAHYKYPRWIEFVDD--LPKTATGKLKR 507
Cdd:PRK05691 524 E--ACQEAPSVVLLLNPgaLPKTSSGKLQR 551
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
159-507 |
7.41e-14 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 73.88 E-value: 7.41e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 159 SDQLEAAPITP-----DDACFWLYSSGSTGQPKgTIHLQHDMVYA-AETygkQVLGIQ---EHDICFSAAKLFFAYGLGN 229
Cdd:PRK07768 136 ADLLAADPIDPvetgeDDLALMQLTSGSTGSPK-AVQITHGNLYAnAEA---MFVAAEfdvETDVMVSWLPLFHDMGMVG 211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 230 GLYFPFSVGATSVLmpVRPV-----PASVYETIARYRPTLYFGvPTLYGSML-------QEEGSLD--GVRLCVSAGEAL 295
Cdd:PRK07768 212 FLTVPMYFGAELVK--VTPMdflrdPLLWAELISKYRGTMTAA-PNFAYALLarrlrrqAKPGAFDlsSLRFALNGAEPI 288
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 296 -PADI--FKRWQQRHG-----------------------------VEIIDGiGSTELSHIFISNLPGKAKPGSS-GKVVP 342
Cdd:PRK07768 289 dPADVedLLDAGARFGlrpeailpaygmaeatlavsfspcgaglvVDEVDA-DLLAALRRAVPATKGNTRRLATlGPPLP 367
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 343 GYDARIVDENFQDIPVGEVGTLLIKGDSAAAAYwnkhqktkSTMLG--------DWVNTDDKYAVDEEGYFYYIGRSNDM 414
Cdd:PRK07768 368 GLEVRVVDEDGQVLPPRGVGVIELRGESVTPGY--------LTMDGfipaqdadGWLDTGDLGYLTEEGEVVVCGRVKDV 439
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 415 LKVGGIWVSPVEVE--ACLIE--HPAvleCAVVGATDDKNLVKPKAFVVLKRGYAPDDQLKKDLKEYVKKTLAHYKY-PR 489
Cdd:PRK07768 440 IIMAGRNIYPTDIEraAARVEgvRPG---NAVAVRLDAGHSREGFAVAVESNAFEDPAEVRRIRHQVAHEVVAEVGVrPR 516
|
410 420
....*....|....*....|
gi 700405679 490 WIEFVD--DLPKTATGKLKR 507
Cdd:PRK07768 517 NVVVLGpgSIPKTPSGKLRR 536
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
169-511 |
9.62e-14 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 73.29 E-value: 9.62e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 169 PDDACFWLYSSGSTGQPKGTIHLQHDMVYAAETYGKQvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMP--- 245
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNS-TEWKTKDRILSWMPLTHDMGLIAFHLAPLIAGMNQYLMPtrl 183
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 246 --VRPV----PASVYET-------------IARYRPTLYFGV-------------PTLYG-----------------SML 276
Cdd:cd05908 184 fiRRPIlwlkKASEHKAtivsspnfgykyfLKTLKPEKANDWdlssirmilngaePIDYElchefldhmskyglkrnAIL 263
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 277 QEEGSLDG-VRLCVS-AGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISnlpgkakpgsSGKVVPGYDARIVDENFQ 354
Cdd:cd05908 264 PVYGLAEAsVGASLPkAQSPFKTITLGRRHVTHGEPEPEVDKKDSECLTFVE----------VGKPIDETDIRICDEDNK 333
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 355 DIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTMLGD-WVNTDDKYAVdEEGYFYYIGRSNDMLKVGGIWVSPVEVEACLIE 433
Cdd:cd05908 334 ILPDGYIGHIQIRGKNVTPGYYNNPEATAKVFTDDgWLKTGDLGFI-RNGRLVITGREKDIIFVNGQNVYPHDIERIAEE 412
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 434 HPAVL--ECAVVGATDDKNLVKPKAFVVLKRgyapddQLKKDLKEYVKKTLAH-YKYPRW----IEFVDDLPKTATGKLK 506
Cdd:cd05908 413 LEGVElgRVVACGVNNSNTRNEEIFCFIEHR------KSEDDFYPLGKKIKKHlNKRGGWqineVLPIRRIPKTTSGKVK 486
|
....*
gi 700405679 507 RFELR 511
Cdd:cd05908 487 RYELA 491
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
177-512 |
1.16e-13 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 72.76 E-value: 1.16e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 177 YSSGSTGQPKGTIHLQHDMVYAAETYGKQvLGIQEHDICFSAAKLFFAYGLGNGLYFPFSVGATSVLMpVRPVPASVYET 256
Cdd:PRK08308 108 YSSGTTGEPKLIRRSWTEIDREIEAYNEA-LNCEQDETPIVACPVTHSYGLICGVLAALTRGSKPVII-TNKNPKFALNI 185
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IARYRPTLYFGVPTLY---GSMLQEEGSLDGVrlcVSAGEALPADIFKRWQQRhGVEIIDGIGSTELSHIFISnlPGKAK 333
Cdd:PRK08308 186 LRNTPQHILYAVPLMLhilGRLLPGTFQFHAV---MTSGTPLPEAWFYKLRER-TTYMMQQYGCSEAGCVSIC--PDMKS 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 334 PGSSGKVVPGYDARI--VDENFQDIPVgevgtllikgdsaaaayWNKHQKTKSTMLGDWvntddkyavDEEGYFYYIGRS 411
Cdd:PRK08308 260 HLDLGNPLPHVSVSAgsDENAPEEIVV-----------------KMGDKEIFTKDLGYK---------SERGTLHFMGRM 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 412 NDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLKRGYAPDDqlkkdLKEYVKKTLAHYKYPRWI 491
Cdd:PRK08308 314 DDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEIDPVQ-----LREWCIQHLAPYQVPHEI 388
|
330 340
....*....|....*....|.
gi 700405679 492 EFVDDLPKTATGKLKRFELRA 512
Cdd:PRK08308 389 ESVTEIPKNANGKVSRKLLEL 409
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
336-511 |
7.38e-13 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 70.41 E-value: 7.38e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 336 SSGKVVPGydARIvdenfqDIPVGEVGTLLIKGDSAAAAYWNKHQKTKstmlGDWVnTDDKYAVDEEGYFYYIGRSNDML 415
Cdd:PRK07445 284 SSGQVLPH--AQI------TIPANQTGNITIQAQSLALGYYPQILDSQ----GIFE-TDDLGYLDAQGYLHILGRNSQKI 350
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 416 KVGGIWVSPVEVEACLIEHPAVLECAVVGATDD---KNLVkpkAFVVLKrgyapDDQLKKD-LKEYVKKTLAHYKYP-RW 490
Cdd:PRK07445 351 ITGGENVYPAEVEAAILATGLVQDVCVLGLPDPhwgEVVT---AIYVPK-----DPSISLEeLKTAIKDQLSPFKQPkHW 422
|
170 180
....*....|....*....|.
gi 700405679 491 IEfVDDLPKTATGKLKRFELR 511
Cdd:PRK07445 423 IP-VPQLPRNPQGKINRQQLQ 442
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
178-509 |
1.10e-12 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 69.79 E-value: 1.10e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIHLQHDMVYAAETYGkQVL---GIQEHDICFSAaklfFAYGL---GNGLY----------FPFSVGats 241
Cdd:COG1541 91 SSGTTGKPTVVGYTRKDLDRWAELFA-RSLraaGVRPGDRVQNA----FGYGLftgGLGLHygaerlgatvIPAGGG--- 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 242 vlMPVRPVpasvyETIARYRPTLYFGVPT--LY-GSMLQEEGsLD----GVRLCVSAGEALPADIFKRWQQRHGVEIIDG 314
Cdd:COG1541 163 --NTERQL-----RLMQDFGPTVLVGTPSylLYlAEVAEEEG-IDprdlSLKKGIFGGEPWSEEMRKEIEERWGIKAYDI 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 315 IGSTELShifisnlPG-----KAKPGSsgkVVPGyDARIVD----ENFQDIPVGEVGTLLIkgdsaaaaywnkhqktksT 385
Cdd:COG1541 235 YGLTEVG-------PGvayecEAQDGL---HIWE-DHFLVEiidpETGEPVPEGEEGELVV------------------T 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 386 MLGDWV------NTDDKYAVDEE-----------GYFyyIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVVGATDD 448
Cdd:COG1541 286 TLTKEAmpliryRTGDLTRLLPEpcpcgrthpriGRI--LGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEYQIVVDRE 363
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 700405679 449 KNLVKPKAFVVLKRGyAPDDQLKKDLKEYVKKTL---AHykyprwIEFV--DDLPKTaTGKLKRFE 509
Cdd:COG1541 364 GGLDELTVRVELAPG-ASLEALAEAIAAALKAVLglrAE------VELVepGSLPRS-EGKAKRVI 421
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
40-509 |
2.05e-11 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 66.08 E-value: 2.05e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMEN--RVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIID 117
Cdd:cd05927 7 SYKEVAERADNIGSALRSLGGKPAPasFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFCD 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 118 E--ELYSkFEPIKDKlrflkhiivvngqapagtisfdsfvaGASDQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHDM 195
Cdd:cd05927 87 AgvKVYS-LEEFEKL--------------------------GKKNKVPPPPPKPEDLATICYTSGTTGNPKGVMLTHGNI 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 196 ---VYAAETYGKQVLGIQEHDICFSAAKL-----------FFAYGLGNGLYfpfsVGATSVLMpvrpvpasvyETIARYR 261
Cdd:cd05927 140 vsnVAGVFKILEILNKINPTDVYISYLPLahifervvealFLYHGAKIGFY----SGDIRLLL----------DDIKALK 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 262 PTLYFGVPTLY-------------GSMLQE---------------EG------------------SLDG-VRLCVSAGEA 294
Cdd:cd05927 206 PTVFPGVPRVLnriydkifnkvqaKGPLKRklfnfalnyklaelrSGvvraspfwdklvfnkikqALGGnVRLMLTGSAP 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 295 LPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVD----ENFQDIPVGEvGTLLIKGDS 370
Cdd:cd05927 286 LSPEVLEFLRVALGCPVLEGYGQTECTAGATLTLPGDTSVGHVGGPLPCAEVKLVDvpemNYDAKDPNPR-GEVCIRGPN 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 371 AAAAYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGRSNDMLKVG-GIWVSPVEVEACLIEHPAVLECAVVGATDD 448
Cdd:cd05927 365 VFSGYYKDPEKTAEALDEDgWLHTGDIGEWLPNGTLKIIDRKKNIFKLSqGEYVAPEKIENIYARSPFVAQIFVYGDSLK 444
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 700405679 449 KNLVkpkAFVVLKRGYAPDDQLKKDLKEYVKKTLAhyKYPRWIEFV-DDLPKTA-TGKLKRFE 509
Cdd:cd05927 445 SFLV---AIVVPDPDVLKEWAASKGGGTGSFEELC--KNPEVKKAIlEDLVRLGkENGLKGFE 502
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
26-512 |
1.64e-09 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 60.48 E-value: 1.64e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 26 RGQKTAILCRD--------NAFTYAQVQAGMNKVGNALKGLGIEMENRVALlllDSEFYPLS---FFGAIKIGAVPICLN 94
Cdd:PLN03052 188 TDDSIAIIWRDegsddlpvNRMTLSELRSQVSRVANALDALGFEKGDAIAI---DMPMNVHAviiYLAIILAGCVVVSIA 264
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 95 TLMQPKDYLYFLNDSRAK-------ILIIDEE--LYSKfepikdklrflkhiiVVNGQAP-----------------AGT 148
Cdd:PLN03052 265 DSFAPSEIATRLKISKAKaiftqdvIVRGGKSipLYSR---------------VVEAKAPkaivlpadgksvrvklrEGD 329
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 149 ISFDSFVAGAS-----DQLEAAPITPDDACFWLYSSGSTGQPKGTIHLQHD-MVYAAETYGKqvLGIQEHDICFSAAKLF 222
Cdd:PLN03052 330 MSWDDFLARANglrrpDEYKAVEQPVEAFTNILFSSGTTGEPKAIPWTQLTpLRAAADAWAH--LDIRKGDIVCWPTNLG 407
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 223 FAYG--LgngLYFPFSVGATSVLMPVRPVPASVYETIARYRPTLYFGVPTLYGSMlQEEGSLDG-----VRLCVSAGEAL 295
Cdd:PLN03052 408 WMMGpwL---VYASLLNGATLALYNGSPLGRGFAKFVQDAKVTMLGTVPSIVKTW-KNTNCMAGldwssIRCFGSTGEAS 483
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 296 PADIFKRWQQRHGVE-IIDGIGSTELSHIFISN-------LPGKAKPGSSGKVVpgydarIVDENFQDIP-----VGEVG 362
Cdd:PLN03052 484 SVDDYLWLMSRAGYKpIIEYCGGTELGGGFVTGsllqpqaFAAFSTPAMGCKLF------ILDDSGNPYPddapcTGELA 557
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 363 ---------TLLIKGDSAAAaYWNKHQKTKSTML---GDWVNTDDKyavdeeGYFYYIGRSNDMLKVGGIWVSPVEVE-A 429
Cdd:PLN03052 558 lfplmfgasSTLLNADHYKV-YFKGMPVFNGKILrrhGDIFERTSG------GYYRAHGRADDTMNLGGIKVSSVEIErV 630
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 430 CLIEHPAVLECAVVGatddknlVKPKA--------FVVLK--RGYAPD-DQLKKDLKEYVKKTLAHYKYPRWIEFVDDLP 498
Cdd:PLN03052 631 CNAADESVLETAAIG-------VPPPGggpeqlviAAVLKdpPGSNPDlNELKKIFNSAIQKKLNPLFKVSAVVIVPSFP 703
|
570
....*....|....
gi 700405679 499 KTATGKLKRFELRA 512
Cdd:PLN03052 704 RTASNKVMRRVLRQ 717
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
176-512 |
7.45e-08 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 54.82 E-value: 7.45e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 176 LYSSGSTGQPKGT-------IHLQHDmvyaaetyGKQVLGIQEHDICFSAAKLFFAYG--LgngLYFPFSVGATSVLMPV 246
Cdd:PLN03051 125 LFSSGTTGEPKAIpwthlspLRCASD--------GWAHMDIQPGDVVCWPTNLGWMMGpwL---LYSAFLNGATLALYGG 193
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 247 RPVPASVYETIARYRPTLYFGVPTLY------GSMLQEEGSLDGVRLCVSAGEALPADIFKRWQQRHGVE--IIDGIGST 318
Cdd:PLN03051 194 APLGRGFGKFVQDAGVTVLGLVPSIVkawrhtGAFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYkpVIEYCGGT 273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 319 ELSHIFISNLPGKAK-PGSSGKVVPGYDARIVDENFQDIP-----VGEVG--TLLIkgdSAAAAYWNK-HQKTKSTMLGD 389
Cdd:PLN03051 274 ELASGYISSTLLQPQaPGAFSTASLGTRFVLLNDNGVPYPddqpcVGEVAlaPPML---GASDRLLNAdHDKVYYKGMPM 350
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 390 WVNTD-------DKYAVDEEGYFYYIGRSNDMLKVGGIWVSPVEVE-ACLIEHPAVLECAVVGATDDKN----LVKPKAF 457
Cdd:PLN03051 351 YGSKGmplrrhgDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIErACDRAVAGIAETAAVGVAPPDGgpelLVIFLVL 430
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 700405679 458 VVLKRGY--APDDQLKKDLKEYVKKTL-AHYKYPRwIEFVDDLPKTATGKLKRFELRA 512
Cdd:PLN03051 431 GEEKKGFdqARPEALQKKFQEAIQTNLnPLFKVSR-VKIVPELPRNASNKLLRRVLRD 487
|
|
| PaaK |
cd05913 |
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ... |
178-509 |
4.03e-07 |
|
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.
Pssm-ID: 341239 [Multi-domain] Cd Length: 425 Bit Score: 52.24 E-value: 4.03e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 178 SSGSTGQPKGTIHLQHD----------MVYAAetygkqvlGIQEHDICFSAaklfFAYGLGNGLyFPFSVGATSVLMPVr 247
Cdd:cd05913 86 SSGTTGKPTVVGYTKNDldvwaelvarCLDAA--------GVTPGDRVQNA----YGYGLFTGG-LGFHYGAERLGALV- 151
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 248 pVPASVYET------IARYRPTLYFGVPT--LY-GSMLQEEGsLDG----VRLCVSAGEALPADIFKRWQQRHGVEIIDG 314
Cdd:cd05913 152 -IPAGGGNTerqlqlIKDFGPTVLCCTPSyaLYlAEEAEEEG-IDPrelsLKVGIFGAEPWTEEMRKRIERRLGIKAYDI 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 315 IGSTElshifiSNLPGKAKP----GSSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKgdsaaaaywnkhQKTKSTM--- 386
Cdd:cd05913 230 YGLTE------IIGPGVAFEceekDGLHIWEDHFIPEIIDpETGEPVPPGEVGELVFT------------TLTKEAMpli 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 387 ---LGDWV-NTDDKYAvdeEGYFY-----YIGRSNDMLKVGGIWVSPVEVEACLIEHPAvlecavvgatddknlVKPKAF 457
Cdd:cd05913 292 ryrTRDITrLLPGPCP---CGRTHrridrITGRSDDMLIIRGVNVFPSQIEDVLLKIPG---------------LGPHYQ 353
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 700405679 458 VVLKR------------------GYAPDDQLKKDLKEYVKKTLA-HYKyprwIEFVD-DLPKTATGKLKRFE 509
Cdd:cd05913 354 LILTRqehldeltikvevrpeadDDEKLEALKQRLERHIKSVLGvTVE----VELVEpGSLPRSEGKAKRVI 421
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
40-452 |
6.03e-07 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 52.33 E-value: 6.03e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALKGLGIEMENRVALLLLDSEFYPLSFFGAIKIGAVPICLNTLMQPKDYLYFLNDSRAKILIIDEE 119
Cdd:PLN02614 81 TYQEVYDIVIKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHSEVSIVFVEEK 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 120 LYSK-FEPIKDKLRFLKHIIVVNGQAP-----AGTI-----SFDSFVA-GASDQLEAAPITPDDACFWLYSSGSTGQPKG 187
Cdd:PLN02614 161 KISElFKTCPNSTEYMKTVVSFGGVSReqkeeAETFglviyAWDEFLKlGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 188 TIhLQHDMVYAAETYGKQVLG-----IQEHDICFSAAKLFFAYG-------LGNGLYFPFSVGATSVLMpvrpvpasvyE 255
Cdd:PLN02614 241 VM-ISNESIVTLIAGVIRLLKsanaaLTVKDVYLSYLPLAHIFDrvieecfIQHGAAIGFWRGDVKLLI----------E 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 256 TIARYRPTLYFGVPTL------------------------------YGSMLQ-------------------EEGSLDGVR 286
Cdd:PLN02614 310 DLGELKPTIFCAVPRVldrvysglqkklsdggflkkfvfdsafsykFGNMKKgqshveasplcdklvfnkvKQGLGGNVR 389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 287 LCVSAGEALPADIFKRWQQRHGVEIIDGIGSTELSHIFISNLPGK-AKPGSSGKVVPGYDAR---IVDENFQDIPVGEVG 362
Cdd:PLN02614 390 IILSGAAPLASHVESFLRVVACCHVLQGYGLTESCAGTFVSLPDElDMLGTVGPPVPNVDIRlesVPEMEYDALASTPRG 469
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 363 TLLIKGDSAAAAYWNKHQKTKSTMLGDWVNTDDKYAVDEEGYFYYIGRSNDMLKVG-GIWVSPVEVEACLIEHPAVLECA 441
Cdd:PLN02614 470 EICIRGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLSqGEYVAVENIENIYGEVQAVDSVW 549
|
490
....*....|.
gi 700405679 442 VVGATDDKNLV 452
Cdd:PLN02614 550 VYGNSFESFLV 560
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
127-410 |
2.68e-06 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 49.97 E-value: 2.68e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 127 IKDKLRFLKH------IIVVNGQAPAGT-------ISFDSFVAGASDQLEAAPITP----DDACFWLYSSGSTGQPKGTI 189
Cdd:PTZ00216 204 VPNLLRLMKSggmpntTIIYLDSLPASVdtegcrlVAWTDVVAKGHSAGSHHPLNIpennDDLALIMYTSGTTGDPKGVM 283
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 190 HLQHDM---VYAAETYGKQVLGIQEHDICFSAaklffayglgnglYFP----FSVGATSVLMpVRPV------PASVYET 256
Cdd:PTZ00216 284 HTHGSLtagILALEDRLNDLIGPPEEDETYCS-------------YLPlahiMEFGVTNIFL-ARGAligfgsPRTLTDT 349
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 257 IAR-------YRPTLYFGVPTLYGSM-------LQEEGSL-----------------DG--------------------- 284
Cdd:PTZ00216 350 FARphgdlteFRPVFLIGVPRIFDTIkkaveakLPPVGSLkrrvfdhayqsrlralkEGkdtpywnekvfsapravlggr 429
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 285 VRLCVSAGEALPAD-------IFKRwqqrhgveIIDGIGSTELSHIFISNLPGKAKPGSSGKVVPGYDARIVD-ENF--- 353
Cdd:PTZ00216 430 VRAMLSGGGPLSAAtqefvnvVFGM--------VIQGWGLTETVCCGGIQRTGDLEPNAVGQLLKGVEMKLLDtEEYkht 501
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 700405679 354 -QDIPVGEV---GTLLIKGdsaaaaYWNKHQKTKSTMLGD-WVNTDDKYAVDEEGYFYYIGR 410
Cdd:PTZ00216 502 dTPEPRGEIllrGPFLFKG------YYKQEELTREVLDEDgWFHTGDVGSIAANGTLRIIGR 557
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
5-419 |
4.21e-06 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 49.34 E-value: 4.21e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 5 LNLPETFNAADYfVDRNIREgRGQKTAILCRD---------NAFTYAQVQAGMNKVGNALKGLGiEMENRVALLLLDSEF 75
Cdd:PRK07769 15 IRFPPNTNLVRH-VERWAKV-RGDKLAYRFLDfsterdgvaRDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLD 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 76 YPLSFFGAIKIG--AVPiclntLMQPK-----DYLY-FLNDSRAK-ILIIDE--ELYSKF---EPIKDKLRflkhIIVVn 141
Cdd:PRK07769 92 YLIAFFGALYAGriAVP-----LFDPAepghvGRLHaVLDDCTPSaILTTTDsaEGVRKFfraRPAKERPR----VIAV- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 142 gqapagtisfDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQHdmvYAAETYGKQV---LGIQEHDICFSA 218
Cdd:PRK07769 162 ----------DAVPDEVGATWVPPEANEDTIAYLQYTSGSTRIPAG-VQITH---LNLPTNVLQVidaLEGQEGDRGVSW 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 219 AKLFFAYGLGN-------GLYFPFSVGATSVLMPVR------PVPASVYETIARyRPTLYFGVPTLYGSMLQEEGSLD-- 283
Cdd:PRK07769 228 LPFFHDMGLITvllpallGHYITFMSPAAFVRRPGRwirelaRKPGGTGGTFSA-APNFAFEHAAARGLPKDGEPPLDls 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 284 GVRLCVSAGEALPADIFKRWQQRHG------VEIIDGIGSTELShIFISNLPGKAKPG---------------------- 335
Cdd:PRK07769 307 NVKGLLNGSEPVSPASMRKFNEAFApyglppTAIKPSYGMAEAT-LFVSTTPMDEEPTviyvdrdelnagrfvevpadap 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 336 ------SSGKVVPGYDARIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTML------------------GDW 390
Cdd:PRK07769 386 navaqvSAGKVGVSEWAVIVDpETASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFQnilksrlseshaegapddALW 465
|
490 500
....*....|....*....|....*....
gi 700405679 391 VNTDDkYAVDEEGYFYYIGRSNDMLKVGG 419
Cdd:PRK07769 466 VRTGD-YGVYFDGELYITGRVKDLVIIDG 493
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
330-429 |
9.54e-06 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 48.40 E-value: 9.54e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 330 GKAKPGSSGKVVP--GYDA------RIVD-ENFQDIPVGEVGTLLIKGDSAAAAYWNKHQKTKSTM------------LG 388
Cdd:PRK05850 357 GHAKRCETGGGTPlvSYGSprsptvRIVDpDTCIECPAGTVGEIWVHGDNVAAGYWQKPEETERTFgatlvdpspgtpEG 436
|
90 100 110 120
....*....|....*....|....*....|....*....|.
gi 700405679 389 DWVNTDDKYAVDEeGYFYYIGRSNDMLKVGGIWVSPVEVEA 429
Cdd:PRK05850 437 PWLRTGDLGFISE-GELFIVGRIKDLLIVDGRNHYPDDIEA 476
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
338-511 |
1.15e-05 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 47.84 E-value: 1.15e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 338 GKVVPGYDARIVDENfQDIPVG--EVGTLLIKGDSAAAAYWNkHQKTKStmlGDWVNTDD-KYAVDeeGYFYYIGRSNDM 414
Cdd:PRK05851 348 GNPIPGMEVRISPGD-GAAGVAgrEIGEIEIRGASMMSGYLG-QAPIDP---DDWFPTGDlGYLVD--GGLVVCGRAKEL 420
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 415 LKVGGIWVSPVEVEACLIEHPAVLECAVVGATDDKNLVKPKAFVVLK-RGyaPDDQLKKdlKEYVKKTLAHYK-YPRWIE 492
Cdd:PRK05851 421 ITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSARPGLVIAAEfRG--PDEAGAR--SEVVQRVASECGvVPSDVV 496
|
170 180
....*....|....*....|.
gi 700405679 493 FVD--DLPKTATGKLKRFELR 511
Cdd:PRK05851 497 FVApgSLPRTSSGKLRRLAVK 517
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
40-429 |
3.78e-05 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 46.27 E-value: 3.78e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 40 TYAQVQAGMNKVGNALK---GLGiemeNRVALLLLDSEFYPLSFFGAIKIG--AVPICLNTLMQPKDYL-YFLNDSRAKI 113
Cdd:PRK12476 70 TWTQLGVRLRAVGARLQqvaGPG----DRVAILAPQGIDYVAGFFAAIKAGtiAVPLFAPELPGHAERLdTALRDAEPTV 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 114 LIIDEelySKFEPIKDKLRFLKHIivvngQAPAgTISFDSFVAGASDQLEAAPITPDDACFWLYSSGSTGQPKGtIHLQH 193
Cdd:PRK12476 146 VLTTT---AAAEAVEGFLRNLPRL-----RRPR-VIAIDAIPDSAGESFVPVELDTDDVSHLQYTSGSTRPPVG-VEITH 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 194 --------DMVYAAETYGKQVLGIqehdicfSAAKLFFAYGLgNGLYFPFSVGATSVLM-PV----RPvpasvYETI--- 257
Cdd:PRK12476 216 ravgtnlvQMILSIDLLDRNTHGV-------SWLPLYHDMGL-SMIGFPAVYGGHSTLMsPTafvrRP-----QRWIkal 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 258 ---ARYRPTLY----F--------GVPT------------LYGSmlqEEGSLDGVRL--CVSAGEALPADIFKRWQqrhg 308
Cdd:PRK12476 283 segSRTGRVVTaapnFayewaaqrGLPAegddidlsnvvlIIGS---EPVSIDAVTTfnKAFAPYGLPRTAFKPSY---- 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 309 veiidGIGSTELshiFISNLPGKAKPG----------------------------SSGKVVPGYDARIVDENFQD-IPVG 359
Cdd:PRK12476 356 -----GIAEATL---FVATIAPDAEPSvvyldreqlgagravrvaadapnavahvSCGQVARSQWAVIVDPDTGAeLPDG 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 360 EVGTLLIKGDSAAAAYWNKHQKTKSTML-------------------GDWVNTDDkYAVDEEGYFYYIGRSNDMLKVGGI 420
Cdd:PRK12476 428 EVGEIWLHGDNIGRGYWGRPEETERTFGaklqsrlaegshadgaaddGTWLRTGD-LGVYLDGELYITGRIADLIVIDGR 506
|
....*....
gi 700405679 421 WVSPVEVEA 429
Cdd:PRK12476 507 NHYPQDIEA 515
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
292-488 |
6.78e-05 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 45.25 E-value: 6.78e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 292 GEALPADIFKRWQQRhGVEIIDGIGSTEL-SHIFisnlpgkAKP--GSS--GKVVPGYDARIVDenfqdipvGEVgtlLI 366
Cdd:PRK09029 249 GAAIPVELTEQAEQQ-GIRCWCGYGLTEMaSTVC-------AKRadGLAgvGSPLPGREVKLVD--------GEI---WL 309
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 367 KGDSAAAAYWnkhQKTKSTMLGD---WVNTDDKYAVDEeGYFYYIGRSNDMLKVGGIWVSPVEVEACLIEHPAVLECAVV 443
Cdd:PRK09029 310 RGASLALGYW---RQGQLVPLVNdegWFATRDRGEWQN-GELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVV 385
|
170 180 190 200
....*....|....*....|....*....|....*....|....*
gi 700405679 444 GATDDKNLVKPKAFVVLKrgyapDDQLKKDLKEYVKKTLAHYKYP 488
Cdd:PRK09029 386 PVADAEFGQRPVAVVESD-----SEAAVVNLAEWLQDKLARFQQP 425
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
104-189 |
2.01e-03 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 40.87 E-value: 2.01e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 700405679 104 YFLNDSRAKILIIDEELYSKFEPIKDKLRFLKHIIVVNGQAPAGTISFDSFVA------------GASDQLEAAPITPDD 171
Cdd:PLN02387 172 HSLNETEVTTVICDSKQLKKLIDISSQLETVKRVIYMDDEGVDSDSSLSGSSNwtvssfseveklGKENPVDPDLPSPND 251
|
90
....*....|....*...
gi 700405679 172 ACFWLYSSGSTGQPKGTI 189
Cdd:PLN02387 252 IAVIMYTSGSTGLPKGVM 269
|
|
| |
