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Conserved domains on  [gi|721872209|gb|KHA31311|]
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hypothetical protein OY36_00020 [Bacillus anthracis]

Protein Classification

globin family protein( domain architecture ID 229384)

globin family protein is an all-helical protein that may bind porphyrins, phycobilins, and other non-heme cofactors, and may play various roles including as a sensor or transporter of oxygen

CATH:  1.10.490.10
Gene Ontology:  GO:0019825|GO:0020037
PubMed:  32863222|16061809|19281958
SCOP:  3000554

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Globin-like super family cl21461
Globin-like protein superfamily; This globin-like domain superfamily contains a wide variety ...
 5-133 4.68e-58

Globin-like protein superfamily; This globin-like domain superfamily contains a wide variety of all-helical proteins that bind porphyrins, phycobilins, and other non-heme cofactors, and play various roles in all three kingdoms of life, including sensors or transporters of oxygen. It includes the M/myoglobin-like, S/sensor globin, and T/truncated globin (TrHb) families, and the phycobiliproteins (PBPs). The M family includes chimeric (FHbs/flavohemoglobins) and single-domain globins: FHbs, Ngbs/neuroglobins, Cygb/cytoglobins, GbE/avian eye specific globin E, GbX/globin X, amphibian GbY/globin Y, Mb/myoglobin, HbA/hemoglobin-alpha, HbB/hemoglobin-beta, SDgbs/single-domain globins related to FHbs, and Adgb/androglobin. The S family includes GCS/globin-coupled sensors, Pgbs/protoglobins, and SSDgbs/sensor single domain globins. The T family is classified into three main groups: TrHb1s (N), TrHb2s (O) and TrHb3s (P). The M- and S families exhibit the canonical secondary structure of hemoglobins, a 3-over-3 alpha-helical sandwich structure (3/3 Mb-fold), built by eight alpha-helical segments (named A through H). For M family Adgbs, this globin domain is permuted, such that C-H are followed by A-B. The T family globins adopt a 2-on-2 alpha-helical sandwich structure, resulting from extensive and complex modifications of the canonical 3-on-3 alpha-helical sandwich that are distributed throughout the whole protein molecule. PBPs bind the linear tetrapyrrole chromophore, phycobilin, a prosthetic group chemically and metabolically related to iron protoporphyrin IX/protoheme. Examples of other globin-like domains which bind non-heme cofactors include those of the Bacillus anthracis sporulation inhibitors pXO1-118 and pXO2-61 which bind fatty acid and halide in vitro, and the globin-like domain of Bacillus subtilis RsbRA which is presumed to channel sensory input to the C-terminal sulfate transporter/ anti-sigma factor antagonist (STAT) domain. RsbRA is a component of the sigma B-activating stressosome, and a regulator of the RNA polymerase sigma factor subunit sigma (B).


The actual alignment was detected with superfamily member pfam09385:

Pssm-ID: 473869  Cd Length: 129  Bit Score: 175.94  E-value: 4.68e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209    5 KCLLCRYLKERQEKFISDWKKKVIIRERDPYKEEIIKNGEHLLSAFIMYLKEEISLQEIEITSKKIARERIDAKVNIAEF 84
Cdd:pfam09385   1 KQVLVDYLEEHQPNFLSEWKKRILISDDDPYKDEIKQNGEALFSLVIEYLRESISLEDLERLAEKIAKERAEANVNIGDF 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 721872209   85 IHNTNVAKIEIMNILTLLNPDLQQYQALVKKINQFFDHLIYYTVHSYYE 133
Cdd:pfam09385  81 VYNVNVGRSEVINYLFKLNVPWDELQPIIDKINECFDKFLYYAVSKYTE 129
 
Name Accession Description Interval E-value
HisK_N pfam09385
Histidine kinase N terminal; This domain is found at the N terminal of sensor histidine kinase ...
 5-133 4.68e-58

Histidine kinase N terminal; This domain is found at the N terminal of sensor histidine kinase proteins.


Pssm-ID: 286473  Cd Length: 129  Bit Score: 175.94  E-value: 4.68e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209    5 KCLLCRYLKERQEKFISDWKKKVIIRERDPYKEEIIKNGEHLLSAFIMYLKEEISLQEIEITSKKIARERIDAKVNIAEF 84
Cdd:pfam09385   1 KQVLVDYLEEHQPNFLSEWKKRILISDDDPYKDEIKQNGEALFSLVIEYLRESISLEDLERLAEKIAKERAEANVNIGDF 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 721872209   85 IHNTNVAKIEIMNILTLLNPDLQQYQALVKKINQFFDHLIYYTVHSYYE 133
Cdd:pfam09385  81 VYNVNVGRSEVINYLFKLNVPWDELQPIIDKINECFDKFLYYAVSKYTE 129
GS_BA2291-HK-like cd14755
Non-heme globin sensor domain of BA2291 histidine kinase and related domains; This subfamily ...
 4-135 3.00e-54

Non-heme globin sensor domain of BA2291 histidine kinase and related domains; This subfamily includes the sensor domain of Bacillus anthracis BA2291 histidine kinase. BA2291 is one of the most active kinases in promoting sporulation, and is found in most members of the Bacillus cereus subfamily of the genus Bacillus, which includes B. anthracis and Bacillus thuringiensis, but not Bacillus subtilis. This subfamily also includes two sensor-only plasmid encoded sporulation inhibitors pXO1-118 and pXO2-61 found only in B. anthracis and various strains of Bacillus cereus having similar plasmids. The pXO1-118 and pXO2-61 sensor domains form homodimers, and in vitro bind fatty acid and halide, and not heme; there may be roles for fatty acid (or similar molecule), chloride ion, and possibly pH, as signaling cues. It has been proposed that BA2291 senses the same environmental cue in vivo, and that pXO1-118 and pXO2-61 act by titrating out an environmental signal that might cause an ill-timed sporulation.


Pssm-ID: 271288  Cd Length: 132  Bit Score: 166.64  E-value: 3.00e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209   4 IKCLLCRYLKERQEKFISDWKKKVIIRERDPYKEEIIKNGEHLLSAFIMYLKEEISLQEIEITSKKIARERIDAKVNIAE 83
Cdd:cd14755    1 AKKRLLDFLEEHQENFLSEWEKRILISEDDPFKEEIRKNGEAMLELVIEYLREEISEEYIKQLAYKVAEERVDANINIGD 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 721872209  84 FIHNTNVAKIEIMNILTLLNPDLQQYQALVKKINQFFDHLIYYTVHSYYEQK 135
Cdd:cd14755   81 FVYNVNLGRSLIFKYLFKLNLPWDELQPIIDKINQCFDKFLYYAVSKYTELK 132
 
Name Accession Description Interval E-value
HisK_N pfam09385
Histidine kinase N terminal; This domain is found at the N terminal of sensor histidine kinase ...
 5-133 4.68e-58

Histidine kinase N terminal; This domain is found at the N terminal of sensor histidine kinase proteins.


Pssm-ID: 286473  Cd Length: 129  Bit Score: 175.94  E-value: 4.68e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209    5 KCLLCRYLKERQEKFISDWKKKVIIRERDPYKEEIIKNGEHLLSAFIMYLKEEISLQEIEITSKKIARERIDAKVNIAEF 84
Cdd:pfam09385   1 KQVLVDYLEEHQPNFLSEWKKRILISDDDPYKDEIKQNGEALFSLVIEYLRESISLEDLERLAEKIAKERAEANVNIGDF 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 721872209   85 IHNTNVAKIEIMNILTLLNPDLQQYQALVKKINQFFDHLIYYTVHSYYE 133
Cdd:pfam09385  81 VYNVNVGRSEVINYLFKLNVPWDELQPIIDKINECFDKFLYYAVSKYTE 129
GS_BA2291-HK-like cd14755
Non-heme globin sensor domain of BA2291 histidine kinase and related domains; This subfamily ...
 4-135 3.00e-54

Non-heme globin sensor domain of BA2291 histidine kinase and related domains; This subfamily includes the sensor domain of Bacillus anthracis BA2291 histidine kinase. BA2291 is one of the most active kinases in promoting sporulation, and is found in most members of the Bacillus cereus subfamily of the genus Bacillus, which includes B. anthracis and Bacillus thuringiensis, but not Bacillus subtilis. This subfamily also includes two sensor-only plasmid encoded sporulation inhibitors pXO1-118 and pXO2-61 found only in B. anthracis and various strains of Bacillus cereus having similar plasmids. The pXO1-118 and pXO2-61 sensor domains form homodimers, and in vitro bind fatty acid and halide, and not heme; there may be roles for fatty acid (or similar molecule), chloride ion, and possibly pH, as signaling cues. It has been proposed that BA2291 senses the same environmental cue in vivo, and that pXO1-118 and pXO2-61 act by titrating out an environmental signal that might cause an ill-timed sporulation.


Pssm-ID: 271288  Cd Length: 132  Bit Score: 166.64  E-value: 3.00e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209   4 IKCLLCRYLKERQEKFISDWKKKVIIRERDPYKEEIIKNGEHLLSAFIMYLKEEISLQEIEITSKKIARERIDAKVNIAE 83
Cdd:cd14755    1 AKKRLLDFLEEHQENFLSEWEKRILISEDDPFKEEIRKNGEAMLELVIEYLREEISEEYIKQLAYKVAEERVDANINIGD 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 721872209  84 FIHNTNVAKIEIMNILTLLNPDLQQYQALVKKINQFFDHLIYYTVHSYYEQK 135
Cdd:cd14755   81 FVYNVNLGRSLIFKYLFKLNLPWDELQPIIDKINQCFDKFLYYAVSKYTELK 132
Globin-like cd01067
Globin-like protein superfamily; This globin-like domain superfamily contains a wide variety ...
 8-127 6.17e-21

Globin-like protein superfamily; This globin-like domain superfamily contains a wide variety of all-helical proteins that bind porphyrins, phycobilins, and other non-heme cofactors, and play various roles in all three kingdoms of life, including sensors or transporters of oxygen. It includes the M/myoglobin-like, S/sensor globin, and T/truncated globin (TrHb) families, and the phycobiliproteins (PBPs). The M family includes chimeric (FHbs/flavohemoglobins) and single-domain globins: FHbs, Ngbs/neuroglobins, Cygb/cytoglobins, GbE/avian eye specific globin E, GbX/globin X, amphibian GbY/globin Y, Mb/myoglobin, HbA/hemoglobin-alpha, HbB/hemoglobin-beta, SDgbs/single-domain globins related to FHbs, and Adgb/androglobin. The S family includes GCS/globin-coupled sensors, Pgbs/protoglobins, and SSDgbs/sensor single domain globins. The T family is classified into three main groups: TrHb1s (N), TrHb2s (O) and TrHb3s (P). The M- and S families exhibit the canonical secondary structure of hemoglobins, a 3-over-3 alpha-helical sandwich structure (3/3 Mb-fold), built by eight alpha-helical segments (named A through H). For M family Adgbs, this globin domain is permuted, such that C-H are followed by A-B. The T family globins adopt a 2-on-2 alpha-helical sandwich structure, resulting from extensive and complex modifications of the canonical 3-on-3 alpha-helical sandwich that are distributed throughout the whole protein molecule. PBPs bind the linear tetrapyrrole chromophore, phycobilin, a prosthetic group chemically and metabolically related to iron protoporphyrin IX/protoheme. Examples of other globin-like domains which bind non-heme cofactors include those of the Bacillus anthracis sporulation inhibitors pXO1-118 and pXO2-61 which bind fatty acid and halide in vitro, and the globin-like domain of Bacillus subtilis RsbRA which is presumed to channel sensory input to the C-terminal sulfate transporter/ anti-sigma factor antagonist (STAT) domain. RsbRA is a component of the sigma B-activating stressosome, and a regulator of the RNA polymerase sigma factor subunit sigma (B).


Pssm-ID: 381255 [Multi-domain]  Cd Length: 119  Bit Score: 81.35  E-value: 6.17e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 721872209   8 LCRYLKERQEKFISDWKKKVI------IRERDPY-KEEIIKNGEHLLSAFIMYLKEEISlqeIEITSKKIARERIDAKVN 80
Cdd:cd01067    1 AWGYLEENQEEIVDDFYDRLFalpslsELFSPPGrLAKCIRKQMHFLRYALYGLVDGDS---IEEGLAGLGEAHKSLGVP 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 721872209  81 IAEFIHNTNVAKIEIMNILTLLNPdlqqyQALVKKINQFFDHLIYYT 127
Cdd:cd01067   78 ISYFIAALNVMKDVLTELLGDKFT-----PAAGEAWTKIFDYIISSM 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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