Crossover junction endodeoxyribonuclease RuvC and similar proteins; The RuvC-like family consists of bacterial RuvC, fungal Cruciform cutting endonuclease 1 (CCE1), bacterial YqgF and monokaryotic chloroplast 1 protein (MOC1). RuvC, CCE1 and MOC1 are Holliday junction resolvases (HJRs), endonucleases that specifically resolve Holliday junction DNA intermediates during homologous recombination. RuvC is part of the RuvABC pathway in Escherichia coli and other Gram-negative bacteria that is involved in processing Holliday junctions, which are formed by the reciprocal exchange of strands between two DNA duplexes. CCE1 is a HJR specific for 4-way junctions; it is involved in the maintenance of mitochondrial DNA. Escherichia coli YqgF has been shown to act as a pre-16S rRNA nuclease, presumably as a monomer. It is involved in the processing of pre-16S rRNA during ribosome maturation. HJRs occur in archaea, bacteria, and in the mitochondria of certain fungi. RuvC and its orthologs are homodimers and display structural similarity to RNase H and Hsp70.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 816655107   7 VVGVDPsmsnfGL--AIGTLDLETDKLDIHGLTLVETKAGGNkktvRVNSDDLRRaneIWRTAKP-----IIEQAHmvfc 79
Cdd:cd22992    1 IIGIDP-----GLsgAIAVLDPDGGLIEVFDMPTLKVGVGGR----RVDAAALAD---LLRELAPeptlaVIEKVG---- 64

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 816655107  80 elPVGSQSSRAQ----TSYGICIGVLACVDKPLIQVTPNEIKHYVGnkLT-TSKEEIIQWATQKQPN-APWLRRK 148
Cdd:cd22992   65 --AMPGQGVQSAfsfgRSYGVIEGVLAALGIPVTLVTPQTWKKAFG--LSgKDKDASRALARRLFPSaASLLKRK 135

Monokaryotic chloroplast 1 protein (MOC1) and similar proteins; Monokaryotic chloroplast 1 protein (MOC1) is a Holliday junction (HJ) resolvase, originally identified from the green algae Chlamydomonas reinhardtii, but was later shown to be genetically conserved among green plants, such as Arabidopsis thaliana and Zea mays. Nicotiana tabacum MOC1 (NtMOC1) is exclusively localized to chloroplasts, and is essential for chloroplast nucleoid segregation. Holliday junction resolvases (HJRs) are endonucleases that specifically resolve Holliday junction DNA intermediates during homologous recombination. NtMOC1 cleaves only four-way junctions and specifically introduces nicks between two cytosines at the homology core. NtMOC1 has the highest structural similarity to RuvC resolvase, suggesting their potentially similar HJ binding and resolution activities. However, the molecular basis for specific HJ resolution by MOC1 remains elusive. This family also includes bacterial homologs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 816655107   7 VVGVDPsmsnfGL--AIGTLDLETDKLDIHGLTLVETKAGGNkktvRVNSDDLRRaneIWRTAKP-----IIEQAHmvfc 79
Cdd:cd22992    1 IIGIDP-----GLsgAIAVLDPDGGLIEVFDMPTLKVGVGGR----RVDAAALAD---LLRELAPeptlaVIEKVG---- 64

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 816655107  80 elPVGSQSSRAQ----TSYGICIGVLACVDKPLIQVTPNEIKHYVGnkLT-TSKEEIIQWATQKQPN-APWLRRK 148
Cdd:cd22992   65 --AMPGQGVQSAfsfgRSYGVIEGVLAALGIPVTLVTPQTWKKAFG--LSgKDKDASRALARRLFPSaASLLKRK 135

Monokaryotic chloroplast 1 protein (MOC1) and similar proteins; Monokaryotic chloroplast 1 protein (MOC1) is a Holliday junction (HJ) resolvase, originally identified from the green algae Chlamydomonas reinhardtii, but was later shown to be genetically conserved among green plants, such as Arabidopsis thaliana and Zea mays. Nicotiana tabacum MOC1 (NtMOC1) is exclusively localized to chloroplasts, and is essential for chloroplast nucleoid segregation. Holliday junction resolvases (HJRs) are endonucleases that specifically resolve Holliday junction DNA intermediates during homologous recombination. NtMOC1 cleaves only four-way junctions and specifically introduces nicks between two cytosines at the homology core. NtMOC1 has the highest structural similarity to RuvC resolvase, suggesting their potentially similar HJ binding and resolution activities. However, the molecular basis for specific HJ resolution by MOC1 remains elusive. This family also includes bacterial homologs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 816655107   7 VVGVDPsmsnfGL--AIGTLDLETDKLDIHGLTLVETKAGGNkktvRVNSDDLRRaneIWRTAKP-----IIEQAHmvfc 79
Cdd:cd22992    1 IIGIDP-----GLsgAIAVLDPDGGLIEVFDMPTLKVGVGGR----RVDAAALAD---LLRELAPeptlaVIEKVG---- 64

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 816655107  80 elPVGSQSSRAQ----TSYGICIGVLACVDKPLIQVTPNEIKHYVGnkLT-TSKEEIIQWATQKQPN-APWLRRK 148
Cdd:cd22992   65 --AMPGQGVQSAfsfgRSYGVIEGVLAALGIPVTLVTPQTWKKAFG--LSgKDKDASRALARRLFPSaASLLKRK 135