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Conserved domains on  [gi|949051541|gb|KRO51967|]
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hypothetical protein ABR78_02480, partial [Acidimicrobiia bacterium BACL6 MAG-120910-bin40]

Protein Classification

PIN domain nuclease( domain architecture ID 13036959)

PIN domain nuclease similar to Mycobacterium tuberculosis VapC15 a two-metal dependent ribonuclease that is neutralized by its cognate antitoxin VapB15

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PIN_MtVapC15-VapC11-like cd18756
VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This ...
 3-128 2.43e-58

VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC11 and VapC15 toxins. M. tuberculosis VapC11 and VapC15 cleave tRNA3 Leu-CAG, VapC11 may additionally cleave tRNA13Leu-GAG and tRNA10Gln-CTG. This subgroup belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


:

Pssm-ID: 350323  Cd Length: 129  Bit Score: 176.26  E-value: 2.43e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLSRpKRIETI--AVDYENAAEI 78
Cdd:cd18756    1 LVDTSAWIEYLRATGSPADREVRELLGEGGpvATTEPVAMELLAGARDEAHLARLRRLLNG-LRLLPLdpALDFEAAAAI 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 949051541  79 YRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18756   80 YRACRRGGHTVRSLIDCLIAAVAIRHGATLLHRDRDFDVIARVTPLRLVS 129
 
Name Accession Description Interval E-value
PIN_MtVapC15-VapC11-like cd18756
VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This ...
 3-128 2.43e-58

VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC11 and VapC15 toxins. M. tuberculosis VapC11 and VapC15 cleave tRNA3 Leu-CAG, VapC11 may additionally cleave tRNA13Leu-GAG and tRNA10Gln-CTG. This subgroup belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350323  Cd Length: 129  Bit Score: 176.26  E-value: 2.43e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLSRpKRIETI--AVDYENAAEI 78
Cdd:cd18756    1 LVDTSAWIEYLRATGSPADREVRELLGEGGpvATTEPVAMELLAGARDEAHLARLRRLLNG-LRLLPLdpALDFEAAAAI 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 949051541  79 YRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18756   80 YRACRRGGHTVRSLIDCLIAAVAIRHGATLLHRDRDFDVIARVTPLRLVS 129
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
2-122 3.51e-17

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 71.82  E-value: 3.51e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   2 ILIDTSAWIEFLRNPLSPYFVEVTKLLGNKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYE---NAAEI 78
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAELEAGELAISAITLAELLYGARRSPDAKRRAALEAFLEAFPVLPFDAEaarRAGEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 949051541  79 YRAGRKIGLTVrSHIDCLIAAVAIRIDAPVLHADH-DFDMISKIT 122
Cdd:COG1487   81 RAALRRAGRPI-GLADLLIAATALAHGLTLVTRNTkDFERIPGLK 124
PIN pfam01850
PIN domain;
2-116 1.65e-08

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 49.33  E-value: 1.65e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541    2 ILIDTSAWIEFLRNplSPYFVEVTKLLGNKSAI--CDPIKMEILAGAR------DEHHLLTLKRLLSRPKRIETIAVDYE 73
Cdd:pfam01850   1 IVLDTSVLIALLRG--EPLHEAARELLEAAGELvtSAIVLAELLYGLNrrlglgKAAELVELLLLLSALEVLPIDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 949051541   74 NAAEIYRAGRKIGLtvrshIDCLIAAVAIRIDAPVLHADHDFD 116
Cdd:pfam01850  79 EAAELRLKYGKLGP-----NDALIAATAKEHGAKLITFDEDFA 116
 
Name Accession Description Interval E-value
PIN_MtVapC15-VapC11-like cd18756
VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This ...
 3-128 2.43e-58

VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC11 and VapC15 toxins. M. tuberculosis VapC11 and VapC15 cleave tRNA3 Leu-CAG, VapC11 may additionally cleave tRNA13Leu-GAG and tRNA10Gln-CTG. This subgroup belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350323  Cd Length: 129  Bit Score: 176.26  E-value: 2.43e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLSRpKRIETI--AVDYENAAEI 78
Cdd:cd18756    1 LVDTSAWIEYLRATGSPADREVRELLGEGGpvATTEPVAMELLAGARDEAHLARLRRLLNG-LRLLPLdpALDFEAAAAI 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 949051541  79 YRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18756   80 YRACRRGGHTVRSLIDCLIAAVAIRHGATLLHRDRDFDVIARVTPLRLVS 129
PIN_MtVapC3-like_start cd09882
VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The ...
 3-125 3.56e-38

VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, and VapC21. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350230  Cd Length: 128  Bit Score: 125.39  E-value: 3.56e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGN--KSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEIYR 80
Cdd:cd09882    1 LVDTSVWIDFFRGRDTPQVERLKELIEEgeDVALTGIILTEVLQGIRDEKEFQRVKEYLLALPILPLTGETYIAAARLYR 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 949051541  81 AGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIK 125
Cdd:cd09882   81 RLRRKGVTVRSTIDCLIAATAIENGLTLLHNDRDFEAIAKHTGLK 125
PIN_MtVapC3-like cd18767
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-127 5.83e-29

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350334  Cd Length: 126  Bit Score: 101.77  E-value: 5.83e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPlsPYFVEVTKLLGNKSAICDPIKMEILAGARDEH------HLLTLKRLLSRPKRIETiavdYENAA 76
Cdd:cd18767    1 LVDTSVWINLFRRP--SRFRLADLNLPDEVVTCLPVVQEVLQGIRDDHafrrirEAMLALPIVESPLSLDV----FLSAA 74

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 949051541  77 EIYRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQH 127
Cdd:cd18767   75 QLYRLARRKGLTVRSSVDCLIAAIAIRHDLTVLHHDRDFDALARISPLKIQ 125
PIN_MtVapC3-like cd18764
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-128 8.23e-29

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350331  Cd Length: 129  Bit Score: 101.50  E-value: 8.23e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVD-YENAAEIY 79
Cdd:cd18764    1 LFDTSVWIDFFNGKETPQTDLLQELLLEDQpvYICPTIVQEILQGIRDDNEFKKIKDLLSALPILELDPYEaAIGAADLY 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 949051541  80 RAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18764   81 RSLRKKGVTIRKSNDCLIAHYAIKFDLPLVHNDRDFDLIAKYSKLKVYK 129
PIN_MtVapC3-like cd18763
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-120 3.18e-28

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350330  Cd Length: 128  Bit Score: 100.04  E-value: 3.18e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEIYR 80
Cdd:cd18763    1 LVDTSVWIDFFRGRDTPQVALLEELIEQREdlCICGVILTEVLQGIRDDKEYAKTKELLESLLFLPMTQSTFIRAADIYR 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 949051541  81 AGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISK 120
Cdd:cd18763   81 TLRKKGITIRKPVDCMIAAVALEHDVSLLHNDRDFDPIAR 120
PIN_MtVapC3-like cd18765
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-121 3.56e-28

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350332  Cd Length: 131  Bit Score: 100.16  E-value: 3.56e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLL--GNKSAICDPIKMEILAGARDEHHLLTLKRLLSR-PKRIETIAVD-YENAAEI 78
Cdd:cd18765    1 LVDTSVWIDLLRGTGTPAVRRLRRLLqsEEPVALAPVILQELLQGARSPEHFVRLQAHFDAlPMLKPKDARAtHRAAAAL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 949051541  79 YRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKI 121
Cdd:cd18765   81 YARCRWAGITPRSPNDCLIAQCAIEHGVPLLHDDRDFETIARV 123
PIN_MtVapC3-like cd18761
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-128 1.15e-26

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350328  Cd Length: 127  Bit Score: 95.89  E-value: 1.15e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNK-SAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEIYRA 81
Cdd:cd18761    1 LIDTSVWISVLRDKTGRVRQQLETLIGGRdVVLTRFTQLELLQGARDEREWQLLSDYLETQDYLEATPSTWSAAARIYFD 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 949051541  82 GRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18761   81 LRRQGLTVRSPIDCCIAQLALENDLTLLHDDRDFETIAQVRPLQQLR 127
PIN_MtVapC3-like cd18758
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-128 7.91e-26

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350325 [Multi-domain]  Cd Length: 127  Bit Score: 93.81  E-value: 7.91e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTK--LLGNKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEIYR 80
Cdd:cd18758    1 LIDTSVWIEALRPRGSPKVKEKVReaLSEDRVATTGIIILELLRGAKTEKEYEELKEDLEALPYLPIEEEVWERAAKLGF 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 949051541  81 AGRKIGLTVRShIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18758   81 DLRRKGLRVPL-TDLLIAAVALKHNCELLHADRHFDLIAKHSPLKVES 127
PIN_MtVapC3-like cd18762
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-121 3.13e-25

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350329  Cd Length: 130  Bit Score: 92.65  E-value: 3.13e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKS--AICDPIKMEILAGARDEHHLLTLKRLLS-----RPKRIETiavDYENA 75
Cdd:cd18762    1 LVDTSVLIDYLKGKDNKPTEKFDEILDRDIpfGINSYIYQEVLQGAKTEKEFNLLKEYLStqrfyHLKDGIE---SYEEA 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 949051541  76 AEIYRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISKI 121
Cdd:cd18762   78 AKIYFRCRRKGITIRSTIDCLIAQTAIENNLYLLHNDRDFDNIAKV 123
PIN_MtVapC3-like cd18760
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-120 6.86e-24

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350327  Cd Length: 126  Bit Score: 89.05  E-value: 6.86e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPyfvEVTKL---LGNKS-AICDPIKMEILAGARDEHHLLTLKRLLSRPKRIET----IAVdyeN 74
Cdd:cd18760    1 LVDSSVWIDYFNGRPTP---QTDRLdalLGREPiLIGDLILTEVLQGFRDDRDFRQARRLLLSLPVVEMlgpeLAI---K 74

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 949051541  75 AAEIYRAGRKIGLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMISK 120
Cdd:cd18760   75 AARNYRALRKKGITIRKTIDVIIATFCIENGHPLLHNDRDFDPFEE 120
PIN_MtVapC3-like cd18759
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-128 1.40e-21

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350326  Cd Length: 126  Bit Score: 83.02  E-value: 1.40e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLL-GNKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAE---- 77
Cdd:cd18759    1 LVDTSVWIEFFRGKSSPVSEVVDELLdEDEVCTCGIVLAELLQGAKSEKELSLLEDFLSALPYLPITDELWIKAGElsfs 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 949051541  78 IYRAGRKIGLTvrshiDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18759   81 LRKKGITIPLS-----DLYIAALAIENNLELFTLDKHFEIIPKHTGLKLYG 126
PIN_MtVapC3-like cd18757
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-128 1.47e-18

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350324  Cd Length: 129  Bit Score: 75.29  E-value: 1.47e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLG----NKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEI 78
Cdd:cd18757    1 LVDTSVWSLALRRKAPDESKIVQELREliedGRVVMIGPIRQELLSGIRDAAQFERLKNKLRAFPDLPLTTEDYEKAAEF 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 949051541  79 YRAGRKIGLTvRSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIKQHQ 128
Cdd:cd18757   81 FNICRSKGIQ-GSNTDFLICAVAVRHDLPIFTTDKDFEHYAKHLPIRLHR 129
PIN_MtVapC3_VapC21-like cd18755
VapC-like PIN domain of Mycobacterium tuberculosis VapC3, VapC21 and related proteins; This ...
 3-122 1.54e-17

VapC-like PIN domain of Mycobacterium tuberculosis VapC3, VapC21 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC3 and VapC21 toxins. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350322  Cd Length: 127  Bit Score: 72.59  E-value: 1.54e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVE--VTKLLgnksAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAEIYR 80
Cdd:cd18755    1 LIDTSALVRLLRPAVAARWEPliERGLV----AICGPTELEILYSARSAADRDRLLRLLRAFPWLPVPDEAWRRALEVQR 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 949051541  81 AgrkigLTVRSH------IDCLIAAVAIRIDAPVLHADHDFDMISKIT 122
Cdd:cd18755   77 L-----LARRGQhraasiPDLLIAATAELHGLTVLHYDRDFDTIAAVT 119
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
2-122 3.51e-17

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 71.82  E-value: 3.51e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   2 ILIDTSAWIEFLRNPLSPYFVEVTKLLGNKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYE---NAAEI 78
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAELEAGELAISAITLAELLYGARRSPDAKRRAALEAFLEAFPVLPFDAEaarRAGEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 949051541  79 YRAGRKIGLTVrSHIDCLIAAVAIRIDAPVLHADH-DFDMISKIT 122
Cdd:COG1487   81 RAALRRAGRPI-GLADLLIAATALAHGLTLVTRNTkDFERIPGLK 124
PIN_MtVapC3-like cd18766
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 3-125 5.43e-12

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350333  Cd Length: 130  Bit Score: 58.60  E-value: 5.43e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVT---KLLGNKSAICDP--IKMEILAGARDEHHLLTLKRLLSRPKRIETIAVDYENAAE 77
Cdd:cd18766    1 LVDTSVWSLALRRPKHAVNREATllkQIIEKGEPIALTgiILQELLQGIRNDKLFDQLKEHLEAFPLILPTREDHIEAAR 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 949051541  78 IYRAGRKIGLTVrSHIDCLIAAVAIRIDAPVLHADHDFDMISKITNIK 125
Cdd:cd18766   81 LRNDCRRKGVQV-SAIDALIAQMCISHDLTLFTTDKDFKHIATHSSLK 127
PIN_VapC4-5_FitB-like cd18741
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 3-118 1.05e-10

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350308  Cd Length: 120  Bit Score: 54.81  E-value: 1.05e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNpLSPYFVEVTKLLGNKSAICDPIKMEILAGARDEHHLLTLKRLLSRPKRI---ETIAVDYENAAEIY 79
Cdd:cd18741    1 LLDTDILIDFLRG-NPSAAEKLLELLGGNLAISVITVAELYAGARNKKELEKLEKLLSLFEVIpvdEEIAELAGELRRKY 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 949051541  80 RAGRKIGLtvrshIDCLIAAVAIRIDAPVLHADH-DFDMI 118
Cdd:cd18741   80 RKSHGLGL-----PDALIAATALEHGLTLVTLNVkHFPMI 114
PIN_VapC4-5_FitB-like cd18753
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 3-121 1.41e-09

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350320  Cd Length: 123  Bit Score: 52.09  E-value: 1.41e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSpyfveVTKLLGNKSAICDPIKM--EILAGA----RDEHHLLTLKRLLSRPkRIETIAVDYENA- 75
Cdd:cd18753    1 LLDTNAYSALLRGDPA-----VLEALARADEIYLPVIVlgELLFGFrkgsREEENRERLEEFLSSP-RVEVLPIDEETAe 74

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 949051541  76 --AEIYRAGRKIGLTVRSHiDCLIAAVAIRIDAPVLHADHDFDMISKI 121
Cdd:cd18753   75 iyAEIKSALKKKGTPIPEN-DIWIAALALEHGLPLVTRDAHFDKIPGL 121
PIN pfam01850
PIN domain;
2-116 1.65e-08

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 49.33  E-value: 1.65e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541    2 ILIDTSAWIEFLRNplSPYFVEVTKLLGNKSAI--CDPIKMEILAGAR------DEHHLLTLKRLLSRPKRIETIAVDYE 73
Cdd:pfam01850   1 IVLDTSVLIALLRG--EPLHEAARELLEAAGELvtSAIVLAELLYGLNrrlglgKAAELVELLLLLSALEVLPIDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 949051541   74 NAAEIYRAGRKIGLtvrshIDCLIAAVAIRIDAPVLHADHDFD 116
Cdd:pfam01850  79 EAAELRLKYGKLGP-----NDALIAATAKEHGAKLITFDEDFA 116
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 3-109 1.90e-08

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350229  Cd Length: 127  Bit Score: 49.02  E-value: 1.90e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYFVEVTKLLGNKSAICDPIKMEILAGA----RDEHHLLTLKRLLSRpkrIETIAVD---YENA 75
Cdd:cd09881    1 LLDTNICIDLLRGRPPSVLERLEELLPEDLAISSITLAELLYGAeksqRPEKNLAALEAFLSR---LEVLPFDeeaAEIY 77

                         90       100       110
                 ....*....|....*....|....*....|....
gi 949051541  76 AEIYRAGRKIGLTVrSHIDCLIAAVAIRIDAPVL 109
Cdd:cd09881   78 GEIRAELEKKGKPI-GDNDLLIAATALAHGLTLV 110
PIN_VapC-FitB-like cd09875
VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin ...
 3-118 3.38e-08

VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin operons, Pyrococcus horikoshii protein PH0500, and other similar bacterial and archaeal homologs; PIN (PilT N terminus) domain-containing proteins of prokaryotic toxin/antitoxin (TA) operons, such as, Mycobacterium tuberculosis VapC of the VapBC (virulence associated proteins) TA operon, and Neisseria gonorrhoeae FitB of the FitAB (fast intracellular trafficking) TA operon, as well as, the archaeal Pyrococcus horikoshii protein PH0500 are included in this family. Toxins of TA operons are believed to be involved in growth inhibition by regulating translation and are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the complex activates the ribonuclease activity of the toxin. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The M. tuberculosis VapC-5 is proposed to be both an endoribonuclease and an exoribonuclease that can act on free RNA in a similar manner to the endo and exonuclease Flap endonuclease-1 (FEN1). VapC-like toxins are structural homologs of FEN1-like PIN domains, but lack the extensive arch/clamp region and the H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region, seen in FEN1-like PIN domains. PIN domains within this group typically contain three or four conserved acidic residues that cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. These putative active site residues are thought to bind Mg2+ and/or Mn2+ ions and be essential for single-stranded ribonuclease activity. VapC-like PIN domains are single domain proteins that form dimers and dimerization configures the active sites in a groove along the long-axis of the structure.


Pssm-ID: 350223  Cd Length: 130  Bit Score: 48.72  E-value: 3.38e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   3 LIDTSAWIEFLRNPLSPYfVEVTKLLGNKSAICDP--------IKMEILAGARDEHHLLTLKRLLSRPKRIETI--AVDY 72
Cdd:cd09875    1 LLDTSVLIWLLDNDPKSH-LRSLADRIERGKVYVSivsrwelaIGVNINKLRLNYAFADLENLLADLAIPVLPLtpRIAD 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 949051541  73 ENAAEIYRAGRKigLTVRSHIDCLIAAVAIRIDAPVLHADHDFDMI 118
Cdd:cd09875   80 EYLEACRNLPLR--GRHRSPFDRMIAAQALQHGFTVAHRDEDFKAI 123
VapC COG1848
VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN ...
 1-122 4.70e-06

VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN domain [Defense mechanisms];


Pssm-ID: 441453  Cd Length: 134  Bit Score: 43.05  E-value: 4.70e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   1 MILIDTSAWIEFLRNPlSPYFVEVTKLL------GNKSAICDPIKMEIL------AGARDEHHLLTLKRLLSrpkRIETI 68
Cdd:COG1848    1 MILLDTNVLIYALEGD-SPFHERARELLeraeegEEELYTSPLVLAEVLyvltrpGGLSLEEARELLEALLS---NIEVV 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 949051541  69 AVDYENAAEIYRAGRKIGLTVRshiDCLIAAVAIRIDAPVLH-ADHDFDMISKIT 122
Cdd:COG1848   77 PVDEEILEEAAELLAKYGLRLN---DALHLATALEHGADALVtFDRDFARVPGLE 128
COG2402 COG2402
Predicted nucleic acid-binding protein, contains PIN domain [General function prediction only]; ...
 1-118 1.80e-04

Predicted nucleic acid-binding protein, contains PIN domain [General function prediction only];


Pssm-ID: 441958  Cd Length: 135  Bit Score: 38.72  E-value: 1.80e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 949051541   1 MILIDTSAWIEFL--RNPLSPYFVEVTKLLGNKSA---ICDPIKMEI--LAGARDEHHL-LTLKRLLSRPKRIETIAV-- 70
Cdd:COG2402    1 MIFVDTGALIALFnrRDQYHEAAVELLESLLEGELplvTTDYVLTETltLLSRRLGREAaVKFLDRLRESGGLEIVPLte 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 949051541  71 -DYENAAEIY--RAGRKIGLTvrshiDCLIAAVAIRID-APVLHADHDFDMI 118
Cdd:COG2402   81 eDFERALELFekYADKPLSLT-----DASSVALMERLGiDEILTFDRDFDQY 127
PIN_VapC4-5_FitB-like cd18751
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 40-108 5.11e-04

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350318  Cd Length: 133  Bit Score: 37.33  E-value: 5.11e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 949051541  40 MEILAGARDEHHLLTLKRLLSRPKRIETIAVDYEN---AAEIY----RAGRKIGLTvrshiDCLIAAVAIRIDAPV 108
Cdd:cd18751   40 MEVVRGLQKVQRAERLQQFLEMIESAEVLPLDRTAaelAGRIYadleRTGQPIGRA-----DPMIAAIALRHGLVL 110
PIN_MtVapC4-C5-like cd18768
VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This ...
 59-124 2.83e-03

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350335  Cd Length: 123  Bit Score: 35.08  E-value: 2.83e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 949051541  59 LSRPKRIETIAVDYENAA-------EIYRAGRKIGLTvrshiDCLIAAVAIRIDAPVLHADHDFDMISKITNI 124
Cdd:cd18768   55 LQLAADLEPLPIDDAVAAawarlrlELRESGRRMRVN-----DSWIAATALAHDLPVVTQDADFDALPDLEVI 122
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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