|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
1-556 |
0e+00 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 1046.92 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMM 80
Cdd:PRK07059 1 MEKIWLKSYPPGVPAEIDASQYPSLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMM 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 81 PNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPvRG 160
Cdd:PRK07059 81 PNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDLLGF-KG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 161 MAVNFVARRVKKLVPPYRLPGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAW 240
Cdd:PRK07059 160 HIVNFVVRRVKKMVPAWSLPGHVRFNDALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQMEAW 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 241 SQPALRKAPQIDALVTVCALPLYHILALNACMLMGARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHP 320
Cdd:PRK07059 240 LQPAFEKKPRPDQLNFVCALPLYHIFALTVCGLLGMRTGGRNILIPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNNP 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 321 GFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDE 400
Cdd:PRK07059 320 DFDKLDFSKLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETSPVATCNPVDATEFSGTIGLPLPSTEVSIRDDDGN 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 401 PVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAA 480
Cdd:PRK07059 400 DLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVAS 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 481 HPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK07059 480 HPGVLEVAAVGVPDEHSGEAVKLFVVKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELRDG 555
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
1-556 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 821.99 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLR-GLRKGARVAVM 79
Cdd:PRK08974 1 MEKVWLNRYPADVPAEINPDRYQSLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALM 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 80 MPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVR 159
Cdd:PRK08974 81 MPNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILTRMGDQLSTAK 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 160 GMAVNFVARRVKKLVPPYRLPGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDA 239
Cdd:PRK08974 161 GTLVNFVVKYIKRLVPKYHLPDAISFRSALHKGRRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQAKA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 240 WSQPALRKAPQIdalvTVCALPLYHILALNACMLMGARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNH 319
Cdd:PRK08974 241 AYGPLLHPGKEL----VVTALPLYHIFALTVNCLLFIELGGQNLLITNPRDIPGFVKELKKYPFTAITGVNTLFNALLNN 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 PGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGEND 399
Cdd:PRK08974 317 EEFQELDFSSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSVNPYDLDYYSGSIGLPVPSTEIKLVDDDG 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 400 EPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVA 479
Cdd:PRK08974 397 NEVPPGEPGELWVKGPQVMLGYWQRPEATDEVIK-DGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVM 475
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 480 AHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK08974 476 LHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELRDE 552
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
1-561 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 728.87 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQ-LRGLRKGARVAVM 79
Cdd:PRK05677 2 IENFWKDKYPAGIAAEINPDEYPNIQAVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQqHTDLKPGDRIAVQ 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 80 MPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVR 159
Cdd:PRK05677 82 LPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTGVKHVIVTEVADMLPPLK 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 160 GMAVNFVARRVKKLVPPYRLPGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDA 239
Cdd:PRK05677 162 RLLINAVVKHVKKMVPAYHLPQAVKFNDALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRA 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 240 WSQPALRKAPQIdalvTVCALPLYHILALNA-CMLMgARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLN 318
Cdd:PRK05677 242 LMGSNLNEGCEI----LIAPLPLYHIYAFTFhCMAM-MLIGNHNILISNPRDLPAMVKELGKWKFSGFVGLNTLFVALCN 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 319 HPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTlGAFTGTIGLPVPSTDIALLGEN 398
Cdd:PRK05677 317 NEAFRKLDFSALKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETSPVVSVNPSQ-AIQVGTIGIPVPSTLCKVIDDD 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 399 DEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVV 478
Cdd:PRK05677 396 GNELPLGEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVL 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 479 AAHPGVIECACVGVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEH 557
Cdd:PRK05677 476 AALPGVLQCAAIGVPDEKSGEAIKVFVVVKpGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRDEE 555
|
....
gi 979216869 558 GQTA 561
Cdd:PRK05677 556 LKKA 559
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
1-555 |
0e+00 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 702.79 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWL--QLRgLRKGARVAV 78
Cdd:PRK08751 3 QARPWLQSYPAGVAAEIDLEQFRTVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLlgELQ-LKKGDRVAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 79 MMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPV 158
Cdd:PRK08751 82 MMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPVKQVITTGLGDMLGFP 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 159 RGMAVNFVARRVKKLVPPYRLPGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQND 238
Cdd:PRK08751 162 KAALVNFVVKYVKKLVPEYRINGAIRFREALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAH 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 239 AWSQPALRKAPQIDALVTvcALPLYHILALNACMLMGARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLN 318
Cdd:PRK08751 242 QWLAGTGKLEEGCEVVIT--ALPLYHIFALTANGLVFMKIGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGLLN 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 319 HPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGEN 398
Cdd:PRK08751 320 TPGFDQIDFSSLKMTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSPAACINPLTLKEYNGSIGLPIPSTDACIKDDA 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 399 DEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVV 478
Cdd:PRK08751 400 GTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVI 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 479 AAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK08751 480 AMMPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRD 556
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
5-555 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 664.98 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 5 WLKSYPEGVPATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLR-GLRKGARVAVMMPNV 83
Cdd:PRK12492 6 WNDKRPAGVPSTIDLAAYKSVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 84 LQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGMAV 163
Cdd:PRK12492 86 LQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVYLNMFGKLVQEVLPDTGIEYLIEAKMGDLLPAAKGWLV 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 164 NFVARRVKKLVPPYRLPGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDA-WSQ 242
Cdd:PRK12492 166 NTVVDKVKKMVPAYHLPQAVPFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVRAcLSQ 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 243 PALRKAPQID--ALVTVCALPLYHILALNA-CMLMGARwGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNH 319
Cdd:PRK12492 246 LGPDGQPLMKegQEVMIAPLPLYHIYAFTAnCMCMMVS-GNHNVLITNPRDIPGFIKELGKWRFSALLGLNTLFVALMDH 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 PGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGEND 399
Cdd:PRK12492 325 PGFKDLDFSALKLTNSGGTALVKATAERWEQLTGCTIVEGYGLTETSPVASTNPYGELARLGTVGIPVPGTALKVIDDDG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 400 EPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVA 479
Cdd:PRK12492 405 NELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVM 484
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 480 AHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK12492 485 AHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRD 560
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
25-554 |
0e+00 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 650.40 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgATP 184
Cdd:cd05936 81 PLYTPRELEHILNDSGAKALIV-------------------------------------------------------AVS 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 FRTALRDGARqPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKApqidaLVTVCALPLYH 264
Cdd:cd05936 106 FTDLLAAGAP-LGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDLLEGD-----DVVLAALPLFH 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 265 ILALNACMLMGARWGAMNLLIPNPRDvDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVV 344
Cdd:cd05936 180 VFGLTVALLLPLALGATIVLIPRFRP-IGVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGAPLPVEV 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 345 STRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNR 424
Cdd:cd05936 259 AERFEELTGVPIVEGYGLTETSPVVAVNPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNR 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 425 PDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLF 504
Cdd:cd05936 339 PEETAEAFV-DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAF 417
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 505 VVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05936 418 VVLKEGAsLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
25-561 |
0e+00 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 517.83 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatp 184
Cdd:COG0318 81 PRLTAEELAYILEDSGARALVT---------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 frtalrdgarqpfqpaalnhddvAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQpaLRKAPqidalVTVCALPLYH 264
Cdd:COG0318 103 -----------------------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALG--LTPGD-----VVLVALPLFH 152
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 265 ILALNACMLMGARWGAMNLLIPNpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVV 344
Cdd:COG0318 153 VFGLTVGLLAPLLAGATLVLLPR-FDPERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVSGGAPLPPEL 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 345 STRWMKTTGCPILEGYGLSETSPSVTLSPP-TLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWN 423
Cdd:COG0318 232 LERFEERFGVRIVEGYGLTETSPVVTVNPEdPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWN 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 424 RPDETAKVMtPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKL 503
Cdd:COG0318 312 DPEATAEAF-RDGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVA 390
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 504 FVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHGQTA 561
Cdd:COG0318 391 FVVLRPGAeLDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGA 449
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
2-556 |
1.10e-157 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 462.55 E-value: 1.10e-157
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 2 ERIWLKSYPEGVPATVDPDRyPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMP 81
Cdd:PRK05605 12 DKPWLQSYAPWTPHDLDYGD-TTLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLP 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 82 NVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGM 161
Cdd:PRK05605 91 NCPQHIVAFYAVLRLGAVVVEHNPLYTAHELEHPFEDHGARVAIVWDKVAPTVERLRRTTPLETIVSVNMIAAMPLLQRL 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 162 AVNFVARRVKK----LVPPyrLPGATPFRTALRD---GARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANI 234
Cdd:PRK05605 171 ALRLPIPALRKaraaLTGP--APGTVPWETLVDAaigGDGSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLFANA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 235 LQNDAWSqPALRKAPQidalVTVCALPLYHILALNACMLMGARWGAMNLLIPNPrDVDGFVKELSRYRVNFFPGVNTLFN 314
Cdd:PRK05605 249 AQGKAWV-PGLGDGPE----RVLAALPMFHAYGLTLCLTLAVSIGGELVLLPAP-DIDLILDAMKKHPPTWLPGVPPLYE 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 315 ALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIAL 394
Cdd:PRK05605 323 KIAEAAEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSPIIVGNPMSDDRRPGYVGVPFPDTEVRI 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 395 LGEND--EPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPN 472
Cdd:PRK05605 403 VDPEDpdETMPDGEEGELLVRGPQVFKGYWNRPEETAKSFL-DGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPA 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 473 EIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRR 551
Cdd:PRK05605 482 EVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAaLDPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRR 561
|
....*
gi 979216869 552 ELRDE 556
Cdd:PRK05605 562 EVREE 566
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
24-556 |
2.60e-143 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 423.55 E-value: 2.60e-143
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNV 103
Cdd:PRK07656 6 TLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPL 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 104 NPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIART-DVRHVVVASLGDLLGPVRGMavnfvarrvkklvppyrlpga 182
Cdd:PRK07656 86 NTRYTADEAAYILARGDAKALFVLGLFLGVDYSATTRLpALEHVVICETEEDDPHTEKM--------------------- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 183 TPFRTALRDGArQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPA-LRKAPQIdalvtVCALP 261
Cdd:PRK07656 145 KTFTDFLAAGD-PAERAPEVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNA---ADWAEYLgLTEGDRY-----LAANP 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 262 LYHIL----ALNACMLMGArwgamnLLIPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGG 336
Cdd:PRK07656 216 FFHVFgykaGVNAPLMRGA------TILPLPVfDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTG 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVVSTRWMKTTGCP-ILEGYGLSETSPSVTLSPPTLGAFT--GTIGLPVPSTDIALLGENDEPVAQGQPGEIAIR 413
Cdd:PRK07656 290 AASMPVALLERFESELGVDiVLTGYGLSEASGVTTFNRLDDDRKTvaGTIGTAIAGVENKIVNELGEEVPVGEVGELLVR 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 414 GPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVP 493
Cdd:PRK07656 370 GPNVMKGYYDDPEATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVP 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 979216869 494 DSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK07656 450 DERLGEVGKAYVVLKPGAeLTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALREK 513
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
1-556 |
4.36e-129 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 389.01 E-value: 4.36e-129
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDPDRYPsLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMM 80
Cdd:PRK06710 3 VEKPWLKSYPEEIPSTISYDIQP-LHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIML 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 81 PNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRG 160
Cdd:PRK06710 82 PNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLDLVFPRVTNVQSATKIEHVIVTRIADFLPFPKN 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 161 MAVNFVARR----VKKLVPPYRLPGATPFRTALRDGARQPFQPaalnHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQ 236
Cdd:PRK06710 162 LLYPFVQKKqsnlVVKVSESETIHLWNSVEKEVNTGVEVPCDP----ENDLALLQYTGGTTGFPKGVMLTHKNLVSNTLM 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 237 NDAWsqpaLRKAPQIDALVtVCALPLYHILALNACMLMGARWGAMNLLIPNpRDVDGFVKELSRYRVNFFPGVNTLFNAL 316
Cdd:PRK06710 238 GVQW----LYNCKEGEEVV-LGVLPFFHVYGMTAVMNLSIMQGYKMVLIPK-FDMKMVFEAIKKHKVTLFPGAPTIYIAL 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 317 LNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLG 396
Cdd:PRK06710 312 LNSPLLKEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSPVTHSNFLWEKRVPGSIGVPWPDTEAMIMS 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 397 -ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIE 475
Cdd:PRK06710 392 lETGEALPPGEIGEIVVKGPQIMKGYWNKPEETAAVLQ-DGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVE 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 476 SVVAAHPGVIECACVGVPDSNSGEAVKLFVV-RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK06710 471 EVLYEHEKVQEVVTIGVPDPYRGETVKAFVVlKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLI 550
|
..
gi 979216869 555 DE 556
Cdd:PRK06710 551 EE 552
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
39-550 |
9.60e-129 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 383.50 E-value: 9.60e-129
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 39 RNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD 118
Cdd:cd17631 11 RTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEVAYILAD 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 119 SGAEAIVvlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfq 198
Cdd:cd17631 91 SGAKVLF------------------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 paalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILqNDAWSQPALRKApqidalVTVCALPLYHILALNACMLMGARW 278
Cdd:cd17631 98 ------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAV-NALAALDLGPDD------VLLVVAPLFHIGGLGVFTLPTLLR 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAMNLLIPNPrDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWmKTTGCPILE 358
Cdd:cd17631 165 GGTVVILRKF-DPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGAPMPERLLRAL-QARGVKFVQ 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTLSPPTLG-AFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGF 437
Cdd:cd17631 243 GYGMTETSPGVTFLSPEDHrRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFR-DGW 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 438 FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEA 516
Cdd:cd17631 322 FHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAeLDEDE 401
|
490 500 510
....*....|....*....|....*....|....
gi 979216869 517 LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:cd17631 402 LIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
20-557 |
5.62e-125 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 376.83 E-value: 5.62e-125
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 20 DRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFT 99
Cdd:PRK06187 3 DYPLTIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 100 VVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTD-VRHVVVASLGDLLGPVrgmavnfvarrvkKLVPPYr 178
Cdd:PRK06187 83 LHPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLPtVRTVIVEGDGPAAPLA-------------PEVGEY- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 179 lpgatpfrTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWsqpaLRKAPQIDALVtvc 258
Cdd:PRK06187 149 --------EELLAAASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAW----LKLSRDDVYLV--- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHILALNACML---MGArwgamNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLG 335
Cdd:PRK06187 214 IVPMFHVHAWGLPYLalmAGA-----KQVIPRRFDPENLLDLIETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIY 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 336 GGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPP---TLGAFT--GTIGLPVPSTDIALLGENDE--PVAQGQPG 408
Cdd:PRK06187 289 GGAALPPALLREFKEKFGIDLVQGYGMTETSPVVSVLPPedqLPGQWTkrRSAGRPLPGVEARIVDDDGDelPPDGGEVG 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 409 EIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECA 488
Cdd:PRK06187 369 EIIVRGPWLMQGYWNRPEATAETID-GGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVA 447
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 489 CVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEH 557
Cdd:PRK06187 448 VIGVPDEKWGERPVAVVVLKPGAtLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLREQY 517
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
29-465 |
2.72e-122 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 366.25 E-value: 2.72e-122
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 29 LDESFATFAARNAYACM-GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLY 107
Cdd:pfam00501 1 LERQAARTPDKTALEVGeGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 108 TPRELEHQLRDSGAEAIVVLENFaTTLQQVIARTDVRHVVVASLGDLLGPVRGMAVNFVARRVKKlvppyrlpgatpfrt 187
Cdd:pfam00501 81 PAEELAYILEDSGAKVLITDDAL-KLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADV--------------- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 188 alrdgarQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPQidaLVTVCALPLYHILA 267
Cdd:pfam00501 145 -------PPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIKRVRPRGFGLGPD---DRVLSTLPLFHDFG 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 268 LNACMLMGARWGAMNLLIP--NPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVS 345
Cdd:pfam00501 215 LSLGLLGPLLAGATVVLPPgfPALDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPLPPELA 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 346 TRWMKTTGCPILEGYGLSETSPSVT--LSPPTLGAFTGTIGLPVPSTDIALLGEND-EPVAQGQPGEIAIRGPQVMAGYW 422
Cdd:pfam00501 295 RRFRELFGGALVNGYGLTETTGVVTtpLPLDEDLRSLGSVGRPLPGTEVKIVDDETgEPVPPGEPGELCVRGPGVMKGYL 374
|
410 420 430 440
....*....|....*....|....*....|....*....|...
gi 979216869 423 NRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS 465
Cdd:pfam00501 375 NDPELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
13-556 |
9.64e-121 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 366.98 E-value: 9.64e-121
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 13 VPATvdpdrypSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQ-LRGLRKGARVAVMMPNVLQYPVAVA 91
Cdd:PRK08314 7 LPET-------SLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYY 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 92 AALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGMAVNFVARRVK 171
Cdd:PRK08314 80 AILRANAVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYLPAEPEIAVPAWLRAEP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 172 KLvPPYRLPGATPFRTALRDGARQPfqPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPAlrkapqI 251
Cdd:PRK08314 160 PL-QALAPGGVVAWKEALAAGLAPP--PHTAGPDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNST------P 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 252 DAlVTVCALPLYHILALNACMLMGARWGAMNLLIPN-PRDVDGfvKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGL 330
Cdd:PRK08314 231 ES-VVLAVLPLFHVTGMVHSMNAPIYAGATVVLMPRwDREAAA--RLIERYRVTHWTNIPTMVVDFLASPGLAERDLSSL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 331 RITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSpSVTLSPPTLGAFTGTIGLPVPSTDIALLG-ENDEPVAQGQPGE 409
Cdd:PRK08314 308 RYIGGGGAAMPEAVAERLKELTGLDYVEGYGLTETM-AQTHSNPPDRPKLQCLGIPTFGVDARVIDpETLEELPPGEVGE 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 410 IAIRGPQVMAGYWNRPDETAKV-MTPDG--FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIE 486
Cdd:PRK08314 387 IVVHGPQVFKGYWNRPEATAEAfIEIDGkrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQE 466
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 487 CACVGVPDSNSGEAVKLFVVRRDPA---LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK08314 467 ACVIATPDPRRGETVKAVVVLRPEArgkTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQEQ 539
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
46-549 |
1.19e-118 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 359.22 E-value: 1.19e-118
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05911 8 GKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIF 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATTLQQviartdvrhvvvaslgdllgpvrgmAVNFVARRVKKLVPPYRLPGATPFRTAL--RDGARQPFQPAALN 203
Cdd:cd05911 88 TDPDGLEKVKE-------------------------AAKELGPKDKIIVLDDKPDGVLSIEDLLspTLGEEDEDLPPPLK 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 H--DDVAFLQYTGGTTGVSKGATLTHRNVIANILQndAWSQPALRKAPQIdalVTVCALPLYHI---LALNACMLMGARw 278
Cdd:cd05911 143 DgkDDTAAILYSSGTTGLPKGVCLSHRNLIANLSQ--VQTFLYGNDGSND---VILGFLPLYHIyglFTTLASLLNGAT- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 gamnLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMA----VQEVVSTRWMKTTgc 354
Cdd:cd05911 217 ----VIIMPKFDSELFLDLIEKYKITFLYLVPPIAAALAKSPLLDKYDLSSLRVILSGGAPlskeLQELLAKRFPNAT-- 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 pILEGYGLSETSPSVTLSPPTlGAFTGTIGLPVPSTDIALLGEN-DEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMT 433
Cdd:cd05911 291 -IKQGYGMTETGGILTVNPDG-DDKPGSVGRLLPNVEAKIVDDDgKDSLGPNEPGEICVRGPQVMKGYYNNPEATKETFD 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 434 PDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALT 513
Cdd:cd05911 369 EDGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKL 448
|
490 500 510
....*....|....*....|....*....|....*...
gi 979216869 514 TEA-LMAYCKKEFTGYKK-PKYIEFRDSLPKSNIGKIL 549
Cdd:cd05911 449 TEKeVKDYVAKKVASYKQlRGGVVFVDEIPKSASGKIL 486
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
206-549 |
1.94e-114 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 343.11 E-value: 1.94e-114
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpALRKAPQIDALVTVCALPLYHIlALNACMLMGARWGAMNLLI 285
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAA-------LAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLL 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 286 PnPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSET 365
Cdd:cd04433 73 P-KFDPEAALELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTET 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 366 SPSVTLSPPTLGAF-TGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVmTPDGFFKSGDVG 444
Cdd:cd04433 152 GGTVATGPPDDDARkPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAV-DEDGWYRTGDLG 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 445 VMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKK 523
Cdd:cd04433 231 RLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGAdLDAEELRAHVRE 310
|
330 340
....*....|....*....|....*.
gi 979216869 524 EFTGYKKPKYIEFRDSLPKSNIGKIL 549
Cdd:cd04433 311 RLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
1-553 |
9.84e-107 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 331.24 E-value: 9.84e-107
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 1 MERIWLKSYPEGVPATVDpdrYPSLAHLLDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARV 76
Cdd:PRK06178 10 LRALQQAAWPAGIPREPE---YPHGERPLTEYLRAWARERpqrpAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 77 AVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLG 156
Cdd:PRK06178 87 AVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAEVLLALDQLAPVVEQVRAETSLRHVIVTSLADVLP 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 157 PVRGMAVNFVARrvkklVPPYRLPGATPFRTALRD-GARQPFQPAALnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIL 235
Cdd:PRK06178 167 AEPTLPLPDSLR-----APRLAAAGAIDLLPALRAcTAPVPLPPPAL--DALAALNYTGGTTGMPKGCEHTQRDMVYTAA 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 236 QNDAWSQPALRKapqidaLVTVCALPLYHILALNACMLMGARWGAMNLLIpNPRDVDGFVKELSRYRVNFFPGVNTLFNA 315
Cdd:PRK06178 240 AAYAVAVVGGED------SVFLSFLPEFWIAGENFGLLFPLFSGATLVLL-ARWDAVAFMAAVERYRVTRTVMLVDNAVE 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 316 LLNHPGFAALDFSGLRITlgggMAVQEV------VSTRWMKTTGCPILEG-YGLSETSPSVTLsppTLGAFTGT------ 382
Cdd:PRK06178 313 LMDHPRFAEYDLSSLRQV----RVVSFVkklnpdYRQRWRALTGSVLAEAaWGMTETHTCDTF---TAGFQDDDfdllsq 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 383 ---IGLPVPSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRK 458
Cdd:PRK06178 386 pvfVGLPVPGTEFKICDfETGELLPLGAEGEIVVRTPSLLKGYWNKPEATAEALR-DGWLHTGDIGKIDEQGFLHYLGRR 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 459 KDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKyIEFR 537
Cdd:PRK06178 465 KEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGAdLTAAALQAWCRENMAVYKVPE-IRIV 543
|
570
....*....|....*.
gi 979216869 538 DSLPKSNIGKILRREL 553
Cdd:PRK06178 544 DALPMTATGKVRKQDL 559
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
46-553 |
1.27e-101 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 316.10 E-value: 1.27e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAE-AI 124
Cdd:cd05904 30 GRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKlAF 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 VVLENFATtlqqvIARTDVRHVVVASlgdllgpvrgmavnfvarrvkklvppYRLPGATPFRTALRDGARQPFQPAaLNH 204
Cdd:cd05904 110 TTAELAEK-----LASLALPVVLLDS--------------------------AEFDSLSFSDLLFEADEAEPPVVV-IKQ 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAwsqpalRKAPQIDAL-VTVCALPLYHILALNACMLMGARWGAMNL 283
Cdd:cd05904 158 DDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVA------GEGSNSDSEdVFLCVLPMFHIYGLSSFALGLLRLGATVV 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIPNpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR-ITLGG---GMAVQEVVSTRWmktTGCPILEG 359
Cdd:cd05904 232 VMPR-FDLEELLAAIERYKVTHLPVVPPIVLALVKSPIVDKYDLSSLRqIMSGAaplGKELIEAFRAKF---PNVDLGQG 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 360 YGLSETSPSVTLSPPTLGAFT--GTIGLPVPSTDIALLGEND-EPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDG 436
Cdd:cd05904 308 YGMTESTGVVAMCFAPEKDRAkyGSVGRLVPNVEAKIVDPETgESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEG 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR-DPALTTE 515
Cdd:cd05904 388 WLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKpGSSLTED 467
|
490 500 510
....*....|....*....|....*....|....*...
gi 979216869 516 ALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05904 468 EIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
49-553 |
2.83e-98 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 305.17 E-value: 2.83e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLe 128
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 nfattlqqviartdvrhvvvASLgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnhDDVA 208
Cdd:cd05935 81 --------------------SEL-----------------------------------------------------DDLA 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPAlrkapqiDALVTVCALPLYHI----------LALNACMLMGARW 278
Cdd:cd05935 88 LIPYTSGTTGLPKGCMHTHFSAAANALQSAVWTGLT-------PSDVILACLPLFHVtgfvgslntaVYVGGTYVLMARW 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 gamnllipnprDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILE 358
Cdd:cd05935 161 -----------DRETALELIEKYKVTFWTNIPTMLVDLLATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVE 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTLSPPTLGAFTgTIGLPVPSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDG- 436
Cdd:cd05935 230 GYGLTETMSQTHTNPPLRPKLQ-CLGIP*FGVDARVIDiETGRELPPNEVGEIVVRGPQIFKGYWNRPEETEESFIEIKg 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 --FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRdPAL-- 512
Cdd:cd05935 309 rrFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLR-PEYrg 387
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 979216869 513 --TTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05935 388 kvTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
24-561 |
4.05e-98 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 307.63 E-value: 4.05e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNV 103
Cdd:PRK08316 12 TIGDILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 104 NPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGMavnfvarrvkklvppyrlpgaT 183
Cdd:PRK08316 92 NFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLGGREAPGGW---------------------L 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 184 PFRTALRDGARQPFQPAaLNHDDVAFLQYTGGTTGVSKGATLTHRNVIAN---------ILQNDawsqpalrkapqidal 254
Cdd:PRK08316 151 DFADWAEAGSVAEPDVE-LADDDLAQILYTSGTESLPKGAMLTHRALIAEyvscivagdMSADD---------------- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 255 VTVCALPLYHILALNaCMLMGARW-GAMNLLIPNPrDVDGFVKELSRYRV-NFF--PgvnTLFNALLNHPGFAALDFSGL 330
Cdd:PRK08316 214 IPLHALPLYHCAQLD-VFLGPYLYvGATNVILDAP-DPELILRTIEAERItSFFapP---TVWISLLRHPDFDTRDLSSL 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 331 RITLGGG--MAVqEVVSTRWMKTTGCPILEGYGLSETSPSVT-LSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQP 407
Cdd:PRK08316 289 RKGYYGAsiMPV-EVLKELRERLPGLRFYNCYGQTEIAPLATvLGPEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEV 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 408 GEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEC 487
Cdd:PRK08316 368 GEIVHRSPQLMLGYWDDPEKTAEAFR-GGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEV 446
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 488 ACVGVPDSNSGEAVKLFVVRRD-PALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHGQTA 561
Cdd:PRK08316 447 AVIGLPDPKWIEAVTAVVVPKAgATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRERYAGAF 521
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
47-559 |
6.21e-98 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 308.58 E-value: 6.21e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:COG0365 38 RTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLIT 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 ---------LENFATTLQQVIART-DVRHVVVaslgdllgpvrgmavnfvarrVKKLVPPYRLPGATPFRTALrDGARQP 196
Cdd:COG0365 118 adgglrggkVIDLKEKVDEALEELpSLEHVIV---------------------VGRTGADVPMEGDLDWDELL-AAASAE 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 197 FQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANI---------LQND---------AWsqpalrkapqidalVTVC 258
Cdd:COG0365 176 FEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAattakyvldLKPGdvfwctadiGW--------------ATGH 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHILALNACMLM--GArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGF--AALDFSGLRITL 334
Cdd:COG0365 242 SYIVYGPLLNGATVVLyeGR---------PDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEplKKYDLSSLRLLG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 335 GGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG 414
Cdd:COG0365 313 SAGEPLNPEVWEWWYEAVGVPIVDGWGQTETGGIFISNLPGLPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKG 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 415 PQ--VMAGYWNRPDETAKVM--TPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACV 490
Cdd:COG0365 393 PWpgMFRGYWNDPERYRETYfgRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVV 472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 491 GVPDSNSGEAVKLFVVRR-----DPALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD-EHGQ 559
Cdd:COG0365 473 GVPDEIRGQVVKAFVVLKpgvepSDELAKE-LQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKiAEGR 546
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
50-555 |
7.69e-94 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 295.76 E-value: 7.69e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLEN 129
Cdd:cd05926 16 TYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTPKG 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 FATTLQQVIARTDVRHVVVASLGdllgpvrgmAVNFVARRVKKLvppyrlpgatPFRTALRDGARqpFQPAALNhDDVAF 209
Cdd:cd05926 96 ELGPASRAASKLGLAILELALDV---------GVLIRAPSAESL----------SNLLADKKNAK--SEGVPLP-DDLAL 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 210 LQYTGGTTGVSKGATLTHRNVIANILqndawsqpALRKAPQIDAL-VTVCALPLYHILALnACMLMGARWGAMNLLIPNP 288
Cdd:cd05926 154 ILHTSGTTGRPKGVPLTHRNLAASAT--------NITNTYKLTPDdRTLVVMPLFHVHGL-VASLLSTLAAGGSVVLPPR 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 289 RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALD-FSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSP 367
Cdd:cd05926 225 FSASTFWPDVRDYNATWYTAVPTIHQILLNRPEPNPESpPPKLRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAH 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 368 SVTLSP-PTLGAFTGTIGLPVpSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVM 446
Cdd:cd05926 305 QMTSNPlPPGPRKPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGWFRTGDLGYL 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 447 DEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEF 525
Cdd:cd05926 384 DADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLReGASVTEEELRAFCRKHL 463
|
490 500 510
....*....|....*....|....*....|
gi 979216869 526 TGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05926 464 AAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
28-556 |
4.04e-93 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 295.57 E-value: 4.04e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 28 LLDESFATFAARNA--YACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNP 105
Cdd:PRK08315 21 LLDRTAARYPDREAlvYRDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 106 LYTPRELEHQLRDSGAEAIVVLENFATT-----LQQVIART--------------DVRHVVvaSLGDllGPVRGMaVNFv 166
Cdd:PRK08315 101 AYRLSELEYALNQSGCKALIAADGFKDSdyvamLYELAPELatcepgqlqsarlpELRRVI--FLGD--EKHPGM-LNF- 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 167 arrvkklvPPYRLPGATPFRTALRdgARQpfqpAALNHDDVAFLQYTGGTTGVSKGATLTHRNvianILQNDAWSQPALR 246
Cdd:PRK08315 175 --------DELLALGRAVDDAELA--ARQ----ATLDPDDPINIQYTSGTTGFPKGATLTHRN----ILNNGYFIGEAMK 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 247 KAPQiDALVtvCALPLYH----ILALNACMLMGArwgAMnlLIPnprdVDGF-----VKELSRYRVNFFPGVNTLFNALL 317
Cdd:PRK08315 237 LTEE-DRLC--IPVPLYHcfgmVLGNLACVTHGA---TM--VYP----GEGFdplatLAAVEEERCTALYGVPTMFIAEL 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 318 NHPGFAALDFSGLRitlGGGMA--------VQEVVSTRWMKTtgcpILEGYGLSETSPSVTLSPPT--LGAFTGTIGLPV 387
Cdd:PRK08315 305 DHPDFARFDLSSLR---TGIMAgspcpievMKRVIDKMHMSE----VTIAYGMTETSPVSTQTRTDdpLEKRVTTVGRAL 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 388 PSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSG 466
Cdd:PRK08315 378 PHLEVKIVDpETGETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGG 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 467 FNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNI 545
Cdd:PRK08315 458 ENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGAtLTEEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVT 537
|
570
....*....|.
gi 979216869 546 GKILRRELRDE 556
Cdd:PRK08315 538 GKIQKFKMREM 548
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
23-554 |
1.77e-92 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 293.05 E-value: 1.77e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 23 PSLAHLLDESFATFAARNAYACMGRTMTYAEL-DTYSRHLGAWLQLrGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVV 101
Cdd:PRK06188 12 ATYGHLLVSALKRYPDRPALVLGDTRLTYGQLaDRISRYIQAFEAL-GLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 102 NVNPLYTPRELEHQLRDSGAEAIVVLEN-FATTLQQVIARTD-VRHVVVaslgdlLGPVrgmavnfvarrvkklvppyrl 179
Cdd:PRK06188 91 ALHPLGSLDDHAYVLEDAGISTLIVDPApFVERALALLARVPsLKHVLT------LGPV--------------------- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 180 PGATPFRTALRDGARQPFQPAALnHDDVAFLQYTGGTTGVSKGATLTHRNVIA-NILQNDAWSQPAlrkapqidALVTVC 258
Cdd:PRK06188 144 PDGVDLLAAAAKFGPAPLVAAAL-PPDIAGLAYTGGTTGKPKGVMGTHRSIATmAQIQLAEWEWPA--------DPRFLM 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHIL-ALNACMLMgaRWGAMNLLipNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGG 337
Cdd:PRK06188 215 CTPLSHAGgAFFLPTLL--RGGTVIVL--AKFDPAEVLRAIEEQRITATFLVPTMIYALLDHPDLRTRDLSSLETVYYGA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 338 MAVQEVVSTRWMKTTGcPIL-EGYGLSEtSPSV--TLS----PPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEI 410
Cdd:PRK06188 291 SPMSPVRLAEAIERFG-PIFaQYYGQTE-APMVitYLRkrdhDPDDPKRLTSCGRPTPGLRVALLDEDGREVAQGEVGEI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 411 AIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACV 490
Cdd:PRK06188 369 CVRGPLVMDGYWNRPEETAEAFR-DGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVI 447
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 491 GVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK06188 448 GVPDEKWGEAVTAVVVLRpGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
47-554 |
5.99e-87 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 278.10 E-value: 5.99e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:cd05959 28 GSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVV 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 LENFATTLQQVIAR--TDVRHVVVAslgdllGPVRGMAvnfvarrvkklvppyrlpgATPFRTALRDGARQPFQPAALNH 204
Cdd:cd05959 108 SGELAPVLAAALTKseHTLVVLIVS------GGAGPEA-------------------GALLLAELVAAEAEQLKPAATHA 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKAPqidALVTVCALPLYHILAL-NACML-MGArwGAMN 282
Cdd:cd05959 163 DDPAFWLYSSGSTGRPKGVVHLHADIYWTA---ELYARNVLGIRE---DDVCFSAAKLFFAYGLgNSLTFpLSV--GATT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYG- 361
Cdd:cd05959 235 VLMPERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSRDLSSLRLCVSAGEALPAEVGERWKARFGLDILDGIGs 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 362 -------LSETSPSVTLspptlgaftGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTp 434
Cdd:cd05959 315 temlhifLSNRPGRVRY---------GTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTFQ- 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 435 DGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR----DP 510
Cdd:cd05959 385 GEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRpgyeDS 464
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 979216869 511 ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05959 465 EALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
26-557 |
1.26e-86 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 277.14 E-value: 1.26e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 26 AHLLDESFATFAARNAYACM---GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVmmpNVLQYPVAVA---AALRAGFT 99
Cdd:PRK07514 3 NNLFDALRAAFADRDAPFIEtpdGLRYTYGDLDAASARLANLLVALGVKPGDRVAV---QVEKSPEALAlylATLRAGAV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 100 VVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVvaSLGDllgpvrgmavnfvarrvkklvppyrl 179
Cdd:PRK07514 80 FLPLNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGAPHVE--TLDA-------------------------- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 180 pGATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQ-NDAWsqpalRKAPQiDALVTvc 258
Cdd:PRK07514 132 -DGTGSLLEAAAAAPDDFETVPRGADDLAAILYTSGTTGRSKGAMLSHGNLLSNALTlVDYW-----RFTPD-DVLIH-- 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHI----LALNACMLMGARwgaMNLLipnPR-DVDGFVKELSRYRVnfFPGVNTLFNALLNHPGFAALDFSGLRIT 333
Cdd:PRK07514 203 ALPIFHThglfVATNVALLAGAS---MIFL---PKfDPDAVLALMPRATV--MMGVPTFYTRLLQEPRLTREAAAHMRLF 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 334 LGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVtlSPPTLGA-FTGTIGLPVPSTDIALLG-ENDEPVAQGQPGEIA 411
Cdd:PRK07514 275 ISGSAPLLAETHREFQERTGHAILERYGMTETNMNT--SNPYDGErRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIE 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:PRK07514 353 VKGPNVFKGYWRMPEKTAEEFRADGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIG 432
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 492 VPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEH 557
Cdd:PRK07514 433 VPHPDFGEGVTAVVVPKpGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLREQY 499
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
8-555 |
2.13e-86 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 278.19 E-value: 2.13e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 8 SYPEGvpATVDPDRYPSLAHLLDESFATFAARNA--YACMGRTMTYAEL-DTYSRHLGAWLQLrGLRKGARVAVMMPNVL 84
Cdd:PRK12583 5 SYYQG--GGDKPLLTQTIGDAFDATVARFPDREAlvVRHQALRYTWRQLaDAVDRLARGLLAL-GVQPGDRVGIWAPNCA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 85 QYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTlqqviartDVRHVVVASLGDLLGPVRGMAVN 164
Cdd:PRK12583 82 EWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICADAFKTS--------DYHAMLQELLPGLAEGQPGALAC 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 165 FVARRVKKLV--PPYRLPGATPFRTALRDG---------ARQpfqpAALNHDDVAFLQYTGGTTGVSKGATLTHRNvian 233
Cdd:PRK12583 154 ERLPELRGVVslAPAPPPGFLAWHELQARGetvsrealaERQ----ASLDRDDPINIQYTSGTTGFPKGATLSHHN---- 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 234 ILQNDAWSQPALRKApqiDALVTVCALPLYH----ILALNACMLMGArwgamNLLIPNPR-DVDGFVKELSRYRVNFFPG 308
Cdd:PRK12583 226 ILNNGYFVAESLGLT---EHDRLCVPVPLYHcfgmVLANLGCMTVGA-----CLVYPNEAfDPLATLQAVEEERCTALYG 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 309 VNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCP-ILEGYGLSETSPSVTLSPPT--LGAFTGTIGL 385
Cdd:PRK12583 298 VPTMFIAELDHPQRGNFDLSSLRTGIMAGAPCPIEVMRRVMDEMHMAeVQIAYGMTETSPVSLQTTAAddLERRVETVGR 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 386 PVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS 465
Cdd:PRK12583 378 TQPHLEVKVVDPDGATVPRGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRG 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 466 GFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV-RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSN 544
Cdd:PRK12583 458 GENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRlHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTV 537
|
570
....*....|.
gi 979216869 545 IGKILRRELRD 555
Cdd:PRK12583 538 TGKVQKFRMRE 548
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
49-553 |
9.88e-85 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 272.12 E-value: 9.88e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLR-GLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVL 127
Cdd:PRK06839 28 MTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLFVE 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 ENFATTLQQVIARTDVRHVVvaslgdllgpvrgmavnfvarrvkklvppyRLPGATPFRTALRDGARQPfqpaalNHDDV 207
Cdd:PRK06839 108 KTFQNMALSMQKVSYVQRVI------------------------------SITSLKEIEDRKIDNFVEK------NESAS 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 208 AFLQYTGGTTGVSKGATLTHRNVIANILQNDAwsqpalrkapQIDAL---VTVCALPLYHILALN----ACMLMGARwga 280
Cdd:PRK06839 152 FIICYTSGTTGKPKGAVLTQENMFWNALNNTF----------AIDLTmhdRSIVLLPLFHIGGIGlfafPTLFAGGV--- 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 mnLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVsTRWMKTTGCPILEGY 360
Cdd:PRK06839 219 --IIVPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCPEEL-MREFIDRGFLFGQGF 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 361 GLSETSPSV-TLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFK 439
Cdd:PRK06839 296 GMTETSPTVfMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEETIQ-DGWLC 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 440 SGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEA-LM 518
Cdd:PRK06839 375 TGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVLIEKdVI 454
|
490 500 510
....*....|....*....|....*....|....*
gi 979216869 519 AYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK06839 455 EHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
46-554 |
1.50e-82 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 264.15 E-value: 1.50e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 Vlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnhd 205
Cdd:cd05934 81 V------------------------------------------------------------------------------- 81
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNvianiLQNDAWSQPALRKAPQIDALVTVcaLPLYHILALnaCMLMGARWGAMNLLI 285
Cdd:cd05934 82 DPASILYTSGTTGPPKGVVITHAN-----LTFAGYYSARRFGLGEDDVYLTV--LPLFHINAQ--AVSVLAALSVGATLV 152
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 286 PNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPgfAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSE 364
Cdd:cd05934 153 LLPRfSASRFWSDVRRYGATVTNYLGAMLSYLLAQP--PSPDDRAHRLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTE 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 365 TSpSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIR---GPQVMAGYWNRPDETAKVMtPDGFFKSG 441
Cdd:cd05934 231 TI-VGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRglrGWGFFKGYYNMPEATAEAM-RNGWFHTG 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 442 DVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAY 520
Cdd:cd05934 309 DLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGEtLDPEELFAF 388
|
490 500 510
....*....|....*....|....*....|....
gi 979216869 521 CKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05934 389 CEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
19-554 |
3.57e-82 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 266.46 E-value: 3.57e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 19 PDRY-PSLAHLLDESFATFAARNAYACM-----GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAA 92
Cdd:PLN02246 15 PDIYiPNHLPLHDYCFERLSEFSDRPCLidgatGRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLG 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 93 ALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDllgpvrgmavnfvarrvkk 172
Cdd:PLN02246 95 ASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVVTIDDPPE------------------- 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 173 lvppyrlpGATPFrTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPQiD 252
Cdd:PLN02246 156 --------GCLHF-SELTQADENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSD-D 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 253 alVTVCALPLYHILALNACMLMGARWGAMNLLIPNpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRI 332
Cdd:PLN02246 226 --VILCVLPMFHIYSLNSVLLCGLRVGAAILIMPK-FEIGALLELIQRHKVTIAPFVPPIVLAIAKSPVVEKYDLSSIRM 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 333 TLGGGMAV-QEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPptlgAF--------TGTIGLPVPSTDIALLG-ENDEPV 402
Cdd:PLN02246 303 VLSGAAPLgKELEDAFRAKLPNAVLGQGYGMTEAGPVLAMCL----AFakepfpvkSGSCGTVVRNAELKIVDpETGASL 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 AQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHP 482
Cdd:PLN02246 379 PRNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHP 458
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 483 GVIECACVGVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PLN02246 459 SIADAAVVPMKDEVAGEVPVAFVVRSnGSEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLR 531
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
25-556 |
2.52e-81 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 264.32 E-value: 2.52e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGftVVNVN 104
Cdd:COG1021 27 LGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAG--AIPVF 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPR--ELEHQLRDSGAEAIVVLE-----NFATTLQQVIART-DVRHVVVAslGDllgpvrgmavnfvarrvkklvpp 176
Cdd:COG1021 105 ALPAHRraEISHFAEQSEAVAYIIPDrhrgfDYRALARELQAEVpSLRHVLVV--GD----------------------- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 177 yrlpgATPFR--TALRDGARQPFQPAaLNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQ-NDAWsqpalrkapQIDA 253
Cdd:COG1021 160 -----AGEFTslDALLAAPADLSEPR-PDPDDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRAsAEIC---------GLDA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 -LVTVCALPLYHILALNACMLMGARW-GAMNLLIPNPRDVDGFvkEL-SRYRVNFFPGVNTLFNALLNHPGFAALDFSGL 330
Cdd:COG1021 225 dTVYLAALPAAHNFPLSSPGVLGVLYaGGTVVLAPDPSPDTAF--PLiERERVTVTALVPPLALLWLDAAERSRYDLSSL 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 331 RITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSE---------TSPSVTLSpptlgaftgTIGLPVPSTD-IALLGENDE 400
Cdd:COG1021 303 RVLQVGGAKLSPELARRVRPALGCTLQQVFGMAEglvnytrldDPEEVILT---------TQGRPISPDDeVRIVDEDGN 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 401 PVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAA 480
Cdd:COG1021 374 PVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLA 453
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 481 HPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCK-KEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:COG1021 454 HPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFLReRGLAAFKLPDRLEFVDALPLTAVGKIDKKALRAA 530
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
15-554 |
1.11e-79 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 262.58 E-value: 1.11e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 15 ATVDPDRyPSLahlldeSFATFAARNAyacMGRTMTYAELD---TYSRHLgawLQLRGLRKGARVAVMMPNVLQYPVAVA 91
Cdd:PRK07529 35 AARHPDA-PAL------SFLLDADPLD---RPETWTYAELLadvTRTANL---LHSLGVGPGDVVAFLLPNLPETHFALW 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 92 AALRAGftVVN-VNPLYTPRELEHQLRDSGAEAIVVLENFATT-----LQQVIAR-TDVRHVVVASLGDLLGPVRGMAVN 164
Cdd:PRK07529 102 GGEAAG--IANpINPLLEPEQIAELLRAAGAKVLVTLGPFPGTdiwqkVAEVLAAlPELRTVVEVDLARYLPGPKRLAVP 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 165 FVARRVKKLVppyrlpgaTPFRTALrdgARQP----FQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANilqndAW 240
Cdd:PRK07529 180 LIRRKAHARI--------LDFDAEL---ARQPgdrlFSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGNEVAN-----AW 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 241 SQPALRKAPQIDalVTVCALPLYHILALNA-CMLMGARWGAMNLLIP----NPRDVDGFVKELSRYRVNFFPGVNTLFNA 315
Cdd:PRK07529 244 LGALLLGLGPGD--TVFCGLPLFHVNALLVtGLAPLARGAHVVLATPqgyrGPGVIANFWKIVERYRINFLSGVPTVYAA 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 316 LLNHPgFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTD--IA 393
Cdd:PRK07529 322 LLQVP-VDGHDISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVSSVNPPDGERRIGSVGLRLPYQRvrVV 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 394 LLGEND---EPVAQGQPGEIAIRGPQVMAGYWNrPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVY 470
Cdd:PRK07529 401 ILDDAGrylRDCAVDEVGVLCIAGPNVFSGYLE-AAHNKGLWLEDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNID 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 471 PNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV-VRRDPALTTEALMAYCKKEFTGYKK-PKYIEFRDSLPKSNIGKI 548
Cdd:PRK07529 480 PAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVqLKPGASATEAELLAFARDHIAERAAvPKHVRILDALPKTAVGKI 559
|
....*.
gi 979216869 549 LRRELR 554
Cdd:PRK07529 560 FKPALR 565
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
25-556 |
2.36e-79 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 258.82 E-value: 2.36e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:PRK07470 9 LAHFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAEAIVVLENFATTLQQV-IARTDVRHVVVASLGdllgpvrgmavnfvarrvkklvppyrlPGAT 183
Cdd:PRK07470 89 FRQTPDEVAYLAEASGARAMICHADFPEHAAAVrAASPDLTHVVAIGGA---------------------------RAGL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 184 PFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRN---VIANILqndAWSQPALrkaPQIDALVTVCal 260
Cdd:PRK07470 142 DYEALVARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGQmafVITNHL---ADLMPGT---TEQDASLVVA-- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 261 PLYHILALNAcMLMGARwGAMNLLIPNPR-DVDGFVKELSRYRV-NFFPgVNTLFNALLNHPGFAALDFSGLRITLGGGM 338
Cdd:PRK07470 214 PLSHGAGIHQ-LCQVAR-GAATVLLPSERfDPAEVWALVERHRVtNLFT-VPTILKMLVEHPAVDRYDHSSLRYVIYAGA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 339 AVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTL-------GAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIA 411
Cdd:PRK07470 291 PMYRADQKRALAKLGKVLVQYFGLGEVTGNITVLPPALhdaedgpDARIGTCGFERTGMEVQIQDDEGRELPPGETGEIC 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:PRK07470 371 VIGPAVFAGYYNNPEANAKAFR-DGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLG 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 492 VPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK07470 450 VPDPVWGEVGVAVCVARDGApVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMVREE 515
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
205-554 |
4.50e-79 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 252.58 E-value: 4.50e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIanilqNDAWSQPALRKAPQIDALVtvCALPLYH----ILALNACMLMGArwgA 280
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIV-----NNGYFIGERLGLTEQDRLC--IPVPLFHcfgsVLGVLACLTHGA---T 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MNLLIP--NPRDVdgfVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCP-IL 357
Cdd:cd05917 72 MVFPSPsfDPLAV---LEAIEKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMKdVT 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPSVTLSPPTLGAFT--GTIGLPVPSTDIALLGENDEPVAQ-GQPGEIAIRGPQVMAGYWNRPDETAKVMTP 434
Cdd:cd05917 149 IAYGMTETSPVSTQTRTDDSIEKrvNTVGRIMPHTEAKIVDPEGGIVPPvGVPGELCIRGYSVMKGYWNDPEKTAEAIDG 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 435 DGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRD-PALT 513
Cdd:cd05917 229 DGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEgAELT 308
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 979216869 514 TEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05917 309 EEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
46-555 |
1.75e-78 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 254.14 E-value: 1.75e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAEL----DTYSRHLGAwlqLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGA 121
Cdd:cd05941 9 GDSITYADLvaraARLANRLLA---LGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVITDSEP 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 122 EAIVvlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaa 201
Cdd:cd05941 86 SLVL---------------------------------------------------------------------------- 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 202 lnhdDVAFLQYTGGTTGVSKGATLTHRNVIANI--LQnDAWsqpalrKAPQIDALVTVcaLPLYHILAL-NAcmLMGARW 278
Cdd:cd05941 90 ----DPALILYTSGTTGRPKGVVLTHANLAANVraLV-DAW------RWTEDDVLLHV--LPLHHVHGLvNA--LLCPLF 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 -GAMNLLIPNPrdvDGFVKELSRY--RVNFFPGVNTLFNALLNHP--------GFAALDFSGLRITLGGGMAVQEVVSTR 347
Cdd:cd05941 155 aGASVEFLPKF---DPKEVAISRLmpSITVFMGVPTIYTRLLQYYeahftdpqFARAAAAERLRLMVSGSAALPVPTLEE 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 348 WMKTTGCPILEGYGLSETSpsVTLSPPTLG-AFTGTIGLPVPSTDIALLGEN-DEPVAQGQPGEIAIRGPQVMAGYWNRP 425
Cdd:cd05941 232 WEAITGHTLLERYGMTEIG--MALSNPLDGeRRPGTVGMPLPGVQARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKP 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 426 DETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS-GFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLF 504
Cdd:cd05941 310 EATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSSVDIIKSgGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAV 389
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 979216869 505 VVRRD--PALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05941 390 VVLRAgaAALSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
14-491 |
2.64e-78 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 258.11 E-value: 2.64e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 14 PATVDPDRYPSLAHLLDESFATFAARNAYACMG----RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVA 89
Cdd:COG1022 2 SEFSDVPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 90 VAAALRAGFTVVnvnPLY---TPRELEHQLRDSGAEAIVVlENfATTLQQVIARTD----VRHVVVASLGDLLGPVRGMA 162
Cdd:COG1022 82 DLAILAAGAVTV---PIYptsSAEEVAYILNDSGAKVLFV-ED-QEQLDKLLEVRDelpsLRHIVVLDPRGLRDDPRLLS 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 163 VNFVARRvkklvppyrlpGAtpfrtALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsq 242
Cdd:COG1022 157 LDELLAL-----------GR-----EVADPAELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARA------ 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 243 palrkAPQIDAL----VTVCALPLYHILA---LNACMLMGARwgaMNLlipnPRDVDGFVKELSRYRVNFFPGV------ 309
Cdd:COG1022 215 -----LLERLPLgpgdRTLSFLPLAHVFErtvSYYALAAGAT---VAF----AESPDTLAEDLREVKPTFMLAVprvwek 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 310 ----------------NTLFNALLNH-------------------PGFAALD---FSGLRITLG--------GGMAVQEV 343
Cdd:COG1022 283 vyagiqakaeeagglkRKLFRWALAVgrryararlagkspslllrLKHALADklvFSKLREALGgrlrfavsGGAALGPE 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 344 VStRWMKTTGCPILEGYGLSETSPSVTLSPPtlGAF-TGTIGLPVPSTDIALlgendepvaqGQPGEIAIRGPQVMAGYW 422
Cdd:COG1022 363 LA-RFFRALGIPVLEGYGLTETSPVITVNRP--GDNrIGTVGPPLPGVEVKI----------AEDGEILVRGPNVMKGYY 429
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 423 NRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMI-LVSGFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:COG1022 430 KNPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIvTSGGKNVAPQPIENALKASPLIEQAVVVG 499
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
48-555 |
1.31e-76 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 248.03 E-value: 1.31e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGaeaivvl 127
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSD------- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 enfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrVKKlvppyrlpgatpfrtalrdgarqpfqpaalnhDDV 207
Cdd:cd05912 74 ------------------------------------------VKL--------------------------------DDI 79
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 208 AFLQYTGGTTGVSKGATLTHRNVIAN---------ILQNDAWsqpalrkapqidalvtVCALPLYHILALNAcMLMGARW 278
Cdd:cd05912 80 ATIMYTSGTTGKPKGVQQTFGNHWWSaigsalnlgLTEDDNW----------------LCALPLFHISGLSI-LMRSVIY 142
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GaMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLN-HPGFAALDFSGlrITLGGGMAVQEVVSTrwMKTTGCPIL 357
Cdd:cd05912 143 G-MTVYLVDKFDAEQVLHLINSGKVTIISVVPTMLQRLLEiLGEGYPNNLRC--ILLGGGPAPKPLLEQ--CKEKGIPVY 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSET-SPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPvaqGQPGEIAIRGPQVMAGYWNRPDETAKVmTPDG 436
Cdd:cd05912 218 QSYGMTETcSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPP---YEVGEILLKGPNVTKGYLNRPDATEES-FENG 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPaLTTEA 516
Cdd:cd05912 294 WFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSERP-ISEEE 372
|
490 500 510
....*....|....*....|....*....|....*....
gi 979216869 517 LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05912 373 LIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
39-554 |
1.56e-75 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 246.22 E-value: 1.56e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 39 RNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD 118
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 119 SGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfq 198
Cdd:cd05919 81 CEARLVVT------------------------------------------------------------------------ 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 paalNHDDVAFLQYTGGTTGVSKGATLTHRNVIaniLQNDAWSQPALRKAPQiDalVTVCALPLYHILALNACMLMGARW 278
Cdd:cd05919 89 ----SADDIAYLLYSSGTTGPPKGVMHAHRDPL---LFADAMAREALGLTPG-D--RVFSSAKMFFGYGLGNSLWFPLAV 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILE 358
Cdd:cd05919 159 GASAVLNPGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILD 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSpSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFF 438
Cdd:cd05919 239 GIGATEVG-HIFLSNRPGAWRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATFN-GGWY 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 439 KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA----LTT 514
Cdd:cd05919 317 RTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAapqeSLA 396
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 979216869 515 EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05919 397 RDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
48-554 |
8.40e-70 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 231.12 E-value: 8.40e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVL 127
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 ENFattlqqviartdvrhvvvaslgdllgpvRGMavnfvarrvkklvppyrlpgatpfrtalrDGARQPfqpaalnhDDV 207
Cdd:cd05903 81 ERF----------------------------RQF-----------------------------DPAAMP--------DAV 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 208 AFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpALRKAPQIDALVTVCALPLYHIL----ALNACMLMGARwgAMNL 283
Cdd:cd05903 96 ALLLFTSGTTGEPKGVMHSHNTLSASIRQ-------YAERLGLGPGDVFLVASPMAHQTgfvyGFTLPLLLGAP--VVLQ 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIPNPRDVdgfVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLS 363
Cdd:cd05903 167 DIWDPDKA---LALMREHGVTFMMGATPFLTDLLNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYGST 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 364 ETSPSVTLSPPTL-GAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDeTAKVMTPDGFFKSGD 442
Cdd:cd05903 244 ECPGAVTSITPAPeDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRGPSVFLGYLDRPD-LTADAAPEGWFRTGD 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 443 VGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYC 521
Cdd:cd05903 323 LARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGAlLTFDELVAYL 402
|
490 500 510
....*....|....*....|....*....|....
gi 979216869 522 KKE-FTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05903 403 DRQgVAKQYWPERLVHVDDLPRTPSGKVQKFRLR 436
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
46-557 |
1.85e-69 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 232.10 E-value: 1.85e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK08276 9 GEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATTLQQVIARTDvRHVVVASLGDllGPVrgmavnfvarrvkklvppyrlPGATPFRTALrdgARQPFQPAAlnhd 205
Cdd:PRK08276 89 VSAALADTAAELAAELP-AGVPLLLVVA--GPV---------------------PGFRSYEEAL---AAQPDTPIA---- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAF---LQYTGGTTGVSKG--ATLTHRNVIANILQNDAwsqPALRKAPQIDALVTVCALPLYHILALNACM---LMGAR 277
Cdd:PRK08276 138 DETAgadMLYSSGTTGRPKGikRPLPGLDPDEAPGMMLA---LLGFGMYGGPDSVYLSPAPLYHTAPLRFGMsalALGGT 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 278 wgamnlLIPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGF--AALDFSGLRITLGGGMAVQEVVSTRWMKTTGc 354
Cdd:PRK08276 215 ------VVVMEKfDAEEALALIERYRVTHSQLVPTMFVRMLKLPEEvrARYDVSSLRVAIHAAAPCPVEVKRAMIDWWG- 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 PIL-EGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTdIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMT 433
Cdd:PRK08276 288 PIIhEYYASSEGGGVTVITSEDWLAHPGSVGKAVLGE-VRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARN 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 434 PDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV-----VRR 508
Cdd:PRK08276 367 PHGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVqpadgADA 446
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 979216869 509 DPALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEH 557
Cdd:PRK08276 447 GDALAAE-LIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLRDRY 494
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
21-555 |
2.03e-69 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 232.04 E-value: 2.03e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 21 RYPSLAHLLDESFAT-FAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFT 99
Cdd:TIGR02262 2 KYNAAEDLLDRNVVEgRGGKTAFIDDISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 100 VVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTD-VRHVVVASLGDllgpvrgmavnfvarrvkklvppyr 178
Cdd:TIGR02262 82 PVALNTLLTADDYAYMLEDSRARVVFVSGALLPVIKAALGKSPhLEHRVVVGRPE------------------------- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 179 lPGATPFRTALRDGARQpFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIAnILQNDAWSQPALRKAPqidalVTVC 258
Cdd:TIGR02262 137 -AGEVQLAELLATESEQ-FKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYW-TAELYARNTLGIREDD-----VCFS 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHILALNACMLMGARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGM 338
Cdd:TIGR02262 209 AAKLFFAYGLGNALTFPMSVGATTVLMGERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPNLPSEDQVRLRLCTSAGE 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 339 AVQEVVSTRWMKTTGCPILEGYGLSETSpSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVM 418
Cdd:TIGR02262 289 ALPAEVGQRWQARFGVDIVDGIGSTEML-HIFLSNLPGDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLISGPSSA 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 419 AGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSG 498
Cdd:TIGR02262 368 TMYWNNRAKSRDTFQ-GEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGL 446
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 499 EAVKLFVVRRD--PALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:TIGR02262 447 IKPKAFVVLRPgqTALETE-LKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
50-555 |
5.85e-69 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 228.76 E-value: 5.85e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVlen 129
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT--- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 fattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalNHDDVAF 209
Cdd:cd05972 79 -------------------------------------------------------------------------DAEDPAL 85
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 210 LQYTGGTTGVSKGATLTHRNVIANILQNDAWSqpALRKapqiDALVTVCALP--LYHIL-ALNACMLMGArwgaMNLLIP 286
Cdd:cd05972 86 IYFTSGTTGLPKGVLHTHSYPLGHIPTAAYWL--GLRP----DDIHWNIADPgwAKGAWsSFFGPWLLGA----TVFVYE 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 287 NPR-DVDGFVKELSRYRVNFFPGVNTLFNaLLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSET 365
Cdd:cd05972 156 GPRfDAERILELLERYGVTSFCGPPTAYR-MLIKQDLSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTET 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 366 SPSVTLSPpTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIR--GPQVMAGYWNRPDETAKVMTpDGFFKSGDV 443
Cdd:cd05972 235 GLTVGNFP-DMPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKlpPPGLFLGYVGDPEKTEASIR-GDYYLTGDR 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 444 GVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALmaycKK 523
Cdd:cd05972 313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEEL----AE 388
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 979216869 524 EFTG--------YKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05972 389 ELQGhvkkvlapYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
46-557 |
6.86e-69 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 229.85 E-value: 6.86e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK03640 25 EKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQLDDAEVKCLI 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATTLQQVIArtdvrhVVVASLGDLlgpvrgmavnfvARRVKKLVPPYRLpgatpfrtalrdgarqpfqpaalnhD 205
Cdd:PRK03640 105 TDDDFEAKLIPGIS------VKFAELMNG------------PKEEAEIQEEFDL-------------------------D 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRN----VIANIL-----QNDAWsqpalrkapqidalvtVCALPLYHILALNACM--LM 274
Cdd:PRK03640 142 EVATIMYTSGTTGKPKGVIQTYGNhwwsAVGSALnlgltEDDCW----------------LAAVPIFHISGLSILMrsVI 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 garWGaMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALL------NHPgfaaldfSGLR-ITLGGGMAVQEVVSTr 347
Cdd:PRK03640 206 ---YG-MRVVLVEKFDAEKINKLLQTGGVTIISVVSTMLQRLLerlgegTYP-------SSFRcMLLGGGPAPKPLLEQ- 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 348 wMKTTGCPILEGYGLSET-SPSVTLSPPTLGAFTGTIGLPVPSTDIALlgENDEPVAQ-GQPGEIAIRGPQVMAGYWNRP 425
Cdd:PRK03640 274 -CKEKGIPVYQSYGMTETaSQIVTLSPEDALTKLGSAGKPLFPCELKI--EKDGVVVPpFEEGEIVVKGPNVTKGYLNRE 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 426 DETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV 505
Cdd:PRK03640 351 DATRETFQ-DGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFV 429
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 979216869 506 VrRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEH 557
Cdd:PRK03640 430 V-KSGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQLV 480
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
206-550 |
1.95e-68 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 224.30 E-value: 1.95e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNviaNILQNDAWSQPA-LRKAPQIdalvtVCALPLYHILALNACMLMGARWGAMnlL 284
Cdd:cd17638 1 DVSDIMFTSGTTGRSKGVMCAHRQ---TLRAAAAWADCAdLTEDDRY-----LIINPFFHTFGYKAGIVACLLTGAT--V 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 285 IPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGC-PILEGYGL 362
Cdd:cd17638 71 VPVAVfDVDAILEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFeTVLTAYGL 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 363 SETSpSVTLSPPTLGAFT--GTIGLPVPSTDIALlgendepvaqGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKS 440
Cdd:cd17638 151 TEAG-VATMCRPGDDAETvaTTCGRACPGFEVRI----------ADDGEVLVRGYNVMQGYLDDPEATAEAIDADGWLHT 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 441 GDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDP-ALTTEALMA 519
Cdd:cd17638 220 GDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGvTLTEEDVIA 299
|
330 340 350
....*....|....*....|....*....|.
gi 979216869 520 YCKKEFTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:cd17638 300 WCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
46-561 |
6.85e-68 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 228.04 E-value: 6.85e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK12406 9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VlenfattlqqviartdvrHVvvaslgDLLGPVR-----GMAVNFVArrvkklVPP-----YRL--------PGATPFRT 187
Cdd:PRK12406 89 A------------------HA------DLLHGLAsalpaGVTVLSVP------TPPeiaaaYRIspalltppAGAIDWEG 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 188 ALRDGA--RQPFQPAALNhddvafLQYTGGTTGVSKGAtlthRNVIANILQNDAWSQPALRKAPQIDALVTVCALPLYHI 265
Cdd:PRK12406 139 WLAQQEpyDGPPVPQPQS------MIYTSGTTGHPKGV----RRAAPTPEQAAAAEQMRALIYGLKPGIRALLTGPLYHS 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 266 lALNACMLMGARWGAmnLLIPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGF--AALDFSGLRITLGGGMAVQE 342
Cdd:PRK12406 209 -APNAYGLRAGRLGG--VLVLQPRfDPEELLQLIERHRITHMHMVPTMFIRLLKLPEEvrAKYDVSSLRHVIHAAAPCPA 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 343 VVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPqVMAG-- 420
Cdd:PRK12406 286 DVKRAMIEWWGPVIYEYYGSTESGAVTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIA-GNPDft 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 421 YWNRPDETAKVmTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEA 500
Cdd:PRK12406 365 YHNKPEKRAEI-DRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEA 443
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 501 VKLfVVRRDP--ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHGQTA 561
Cdd:PRK12406 444 LMA-VVEPQPgaTLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDPYWANA 505
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
50-555 |
1.02e-67 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 227.90 E-value: 1.02e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLEN 129
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVVFVDRD 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 FATTLQQVIARTD-VRHVVVASLGDLLGPVRGMAVNFVARRVKKLVPPYRLPGatpfrtalrdgarqpfqpaaLNHDDVA 208
Cdd:cd12119 107 FLPLLEAIAPRLPtVEHVVVMTDDAAMPEPAGVGVLAYEELLAAESPEYDWPD--------------------FDENTAA 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRnviANILQNDAWSQPALRKAPQIDalVTVCALPLYHILALN---ACMLMGARwgamnLLI 285
Cdd:cd12119 167 AICYTSGTTGNPKGVVYSHR---SLVLHAMAALLTDGLGLSESD--VVLPVVPMFHVNAWGlpyAAAMVGAK-----LVL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 286 PNPRDVDGFVKEL-SRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWmKTTGCPILEGYGLSE 364
Cdd:cd12119 237 PGPYLDPASLAELiEREGVTFAAGVPTVWQGLLDHLEANGRDLSSLRRVVIGGSAVPRSLIEAF-EERGVRVIHAWGMTE 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 365 TSPSVTLSPPTLG----------AFTGTIGLPVPSTDIALLGENDEPV-AQGQP-GEIAIRGPQVMAGYWNRPDETAKvM 432
Cdd:cd12119 316 TSPLGTVARPPSEhsnlsedeqlALRAKQGRPVPGVELRIVDDDGRELpWDGKAvGELQVRGPWVTKSYYKNDEESEA-L 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA- 511
Cdd:cd12119 395 TEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGAt 474
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 979216869 512 LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd12119 475 VTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
46-555 |
2.72e-67 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 225.84 E-value: 2.72e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNplytprelehqLRDSGAEaiv 125
Cdd:PRK09088 20 GRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLN-----------WRLSASE--- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfattLQQVIARTDVRhVVVASLGDLLGPVRGMAVNFVARRVKKLVPPYRlpgatpfrtalrdgarqpfqpAALNHD 205
Cdd:PRK09088 86 --------LDALLQDAEPR-LLLGDDAVAAGRTDVEDLAAFIASADALEPADT---------------------PSIPPE 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNvianiLQNDAWSQPALRKapqIDAL-VTVCALPLYHILAL----NACMLMGarwGA 280
Cdd:PRK09088 136 RVSLILFTSGTSGQPKGVMLSERN-----LQQTAHNFGVLGR---VDAHsSFLCDAPMFHIIGLitsvRPVLAVG---GS 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MnlLIPN---PRDVDGFVKELSRYRVNFFpGVNTLFNALLNHPGFAALDFSGLRITLGGGmAVQEVVSTRWMKTTGCPIL 357
Cdd:PRK09088 205 I--LVSNgfePKRTLGRLGDPALGITHYF-CVPQMAQAFRAQPGFDAAALRHLTALFTGG-APHAAEDILGWLDDGIPMV 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSE--TSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPD 435
Cdd:PRK09088 281 DGFGMSEagTVFGMSVDCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGD 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 436 GFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDP-ALTT 514
Cdd:PRK09088 361 GWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGaPLDL 440
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 979216869 515 EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK09088 441 ERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRD 481
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
46-556 |
2.80e-67 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 227.32 E-value: 2.80e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK06087 47 GASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFF 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATT--LQQVIA-RTDVRHVVVASLGDLLGPvrgmAVNFVArrVKKLVPPYrlpgaTPFRTalrdgarqpfqPAAL 202
Cdd:PRK06087 127 APTLFKQTrpVDLILPlQNQLPQLQQIVGVDKLAP----ATSSLS--LSQIIADY-----EPLTT-----------AITT 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTHRNVIA---------NILQNDAWSQPAlrkapqidalvtvcalPLYH----ILALN 269
Cdd:PRK06087 185 HGDELAAVLFTSGTEGLPKGVMLTHNNILAseraycarlNLTWQDVFMMPA----------------PLGHatgfLHGVT 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 270 ACMLMGARwgAMNLLIPNPrdvDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVsTRWM 349
Cdd:PRK06087 249 APFLIGAR--SVLLDIFTP---DACLALLEQQRCTCMLGATPFIYDLLNLLEKQPADLSALRFFLCGGTTIPKKV-AREC 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPILEGYGLSETSPSVTLSP-PTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDET 428
Cdd:PRK06087 323 QQRGIKLLSVYGSTESSPHAVVNLdDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELT 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 429 AKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR 508
Cdd:PRK06087 403 ARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLK 482
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 509 DP--ALTTEALMAY-CKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK06087 483 APhhSLTLEEVVAFfSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKD 533
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
46-554 |
1.63e-66 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 223.33 E-value: 1.63e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAwlQLRGLRkgaRVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAiv 125
Cdd:PRK07787 23 GRVLSRSDLAGAATAVAE--RVAGAR---RVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADSGAQA-- 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfattlqqviartdvrhvvvaslgdLLGPvrgmavnfvarrvkklvPPYRLPG--ATPFRTALRDGARQPFQPAaln 203
Cdd:PRK07787 96 ----------------------------WLGP-----------------APDDPAGlpHVPVRLHARSWHRYPEPDP--- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 hDDVAFLQYTGGTTGVSKGATLTHRNVIANI--LQnDAWSQPAlrkapqIDALVTvcALPLYHILALNACMLMGARWG-- 279
Cdd:PRK07787 128 -DAPALIVYTSGTTGPPKGVVLSRRAIAADLdaLA-EAWQWTA------DDVLVH--GLPLFHVHGLVLGVLGPLRIGnr 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 280 AMNLLIPNPrdvDGFVKELSRYRVNFFpGVNTLFNALLNHPGfAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEG 359
Cdd:PRK07787 198 FVHTGRPTP---EAYAQALSEGGTLYF-GVPTVWSRIAADPE-AARALRGARLLVSGSAALPVPVFDRLAALTGHRPVER 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 360 YGLSETSpsVTLSPPTLGAF-TGTIGLPVPSTDIALLGENDEPVAQGQP--GEIAIRGPQVMAGYWNRPDETAKVMTPDG 436
Cdd:PRK07787 273 YGMTETL--ITLSTRADGERrPGWVGLPLAGVETRLVDEDGGPVPHDGEtvGELQVRGPTLFDGYLNRPDATAAAFTADG 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 FFKSGDVGVMDEHGFVRIVDRKK-DMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPAlTTE 515
Cdd:PRK07787 351 WFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDV-AAD 429
|
490 500 510
....*....|....*....|....*....|....*....
gi 979216869 516 ALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK07787 430 ELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
212-550 |
1.37e-65 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 216.75 E-value: 1.37e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 212 YTGGTTGVSKGATLTHRNVIANILQ-NDAWSqpaLRKAPqidalVTVCALPLYHILALN---ACMLMGARwgamNLLIPN 287
Cdd:cd17637 7 HTAAVAGRPRGAVLSHGNLIAANLQlIHAMG---LTEAD-----VYLNMLPLFHIAGLNlalATFHAGGA----NVVMEK 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 288 pRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGggMAVQEVVStRWMKTTGCPILEGYGLSETSP 367
Cdd:cd17637 75 -FDPAEALELIEEEKVTLMGSFPPILSNLLDAAEKSGVDLSSLRHVLG--LDAPETIQ-RFEETTGATFWSLYGQTETSG 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 368 SVTLSPPTlgAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVmTPDGFFKSGDVGVMD 447
Cdd:cd17637 151 LVTLSPYR--ERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYT-FRNGWHHTGDLGRFD 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 448 EHGFVRIVDRK--KDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKE 524
Cdd:cd17637 228 EDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGAtLTADELIEFVGSR 307
|
330 340
....*....|....*....|....*.
gi 979216869 525 FTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:cd17637 308 IARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
45-558 |
3.88e-65 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 221.57 E-value: 3.88e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 45 MGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAI 124
Cdd:PRK07786 39 LGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVV 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 V---VLENFATTLQQVIARTDVrhVVVASlgdllGPVRGMAVNFvarrvkklvppyrlpgatpfrTALRDGARQPFQPAA 201
Cdd:PRK07786 119 VteaALAPVATAVRDIVPLLST--VVVAG-----GSSDDSVLGY---------------------EDLLAEAGPAHAPVD 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 202 LNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpALRK-APQIDALVTVCALPLYHILALNAcMLMGARWGA 280
Cdd:PRK07786 171 IPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMT-------CLRTnGADINSDVGFVGVPLFHIAGIGS-MLPGLLLGA 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MNLLIP-NPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSgLRItLGGGMAVQEVVSTRWMKTT--GCPIL 357
Cdd:PRK07786 243 PTVIYPlGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQARPRDLA-LRV-LSWGAAPASDTLLRQMAATfpEAQIL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPsVT--LSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpD 435
Cdd:PRK07786 321 AAFGQTEMSP-VTcmLLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAFA-G 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 436 GFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA--LT 513
Cdd:PRK07786 399 GWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDaaLT 478
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 979216869 514 TEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHG 558
Cdd:PRK07786 479 LEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRERYG 523
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
14-560 |
2.86e-63 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 216.77 E-value: 2.86e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 14 PATVDPDRYpSLAHLLDESFATFAARNAY--ACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVA 91
Cdd:PLN02330 20 PSVPVPDKL-TLPDFVLQDAELYADKVAFveAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVAL 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 92 AALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVvlenfattlqqviarTDVRHvvvaslgdlLGPVRGMAVNFVarrvk 171
Cdd:PLN02330 99 GIMAAGGVFSGANPTALESEIKKQAEAAGAKLIV---------------TNDTN---------YGKVKGLGLPVI----- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 172 kLVPPYRLPGATPFRTALRDGARQPFQPA--ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALrkap 249
Cdd:PLN02330 150 -VLGEEKIEGAVNWKELLEAADRAGDTSDneEILQTDLCALPFSSGTTGISKGVMLTHRNLVANLCSSLFSVGPEM---- 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 250 qIDALVTVCALPLYHILALNACMLMGARwGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSG 329
Cdd:PLN02330 225 -IGQVVTLGLIPFFHIYGITGICCATLR-NKGKVVVMSRFELRTFLNALITQEVSFAPIVPPIILNLVKNPIVEEFDLSK 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 330 LR---ITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETS-PSVTLSPPTLG---AFTGTIGLPVPSTDIALLG-ENDEP 401
Cdd:PLN02330 303 LKlqaIMTAAAPLAPELLTAFEAKFPGVQVQEAYGLTEHScITLTHGDPEKGhgiAKKNSVGFILPNLEVKFIDpDTGRS 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 402 VAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAH 481
Cdd:PLN02330 383 LPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTH 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 482 PGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEA-LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHGQT 560
Cdd:PLN02330 463 PSVEDAAVVPLPDEEAGEIPAACVVINPKAKESEEdILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKEKMLSI 542
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
47-491 |
1.30e-61 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 209.76 E-value: 1.30e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLY---TPRELEHQLRDSGAEA 123
Cdd:cd05907 4 QPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPV---PIYptsSAEQIAYILNDSEAKA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 124 IVVlenfattlqqviartdvrhvvvaslGDLlgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaaln 203
Cdd:cd05907 81 LFV-------------------------EDP------------------------------------------------- 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 hDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAwsqpALRKAPQiDALVTVcaLPLYHILA----LNACMLMGARwg 279
Cdd:cd05907 87 -DDLATIIYTSGTTGRPKGVMLSHRNILSNALALAE----RLPATEG-DRHLSF--LPLAHVFErragLYVPLLAGAR-- 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 280 amnllIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLN------HPG-----FAALDFSGLRITLGGGMAVQEVVStRW 348
Cdd:cd05907 157 -----IYFASSAETLLDDLSEVRPTVFLAVPRVWEKVYAaikvkaVPGlkrklFDLAVGGRLRFAASGGAPLPAELL-HF 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 349 MKTTGCPILEGYGLSETSPSVTLSPPTLGAFtGTIGLPVPSTDIALlgendepvaqGQPGEIAIRGPQVMAGYWNRPDET 428
Cdd:cd05907 231 FRALGIPVYEGYGLTETSAVVTLNPPGDNRI-GTVGKPLPGVEVRI----------ADDGEILVRGPNVMLGYYKNPEAT 299
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 979216869 429 AKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS-GFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:cd05907 300 AEALDADGWLHTGDLGEIDEDGFLHITGRKKDLIITSgGKNISPEPIENALKASPLISQAVVIG 363
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
46-555 |
7.45e-61 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 208.72 E-value: 7.45e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQlRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05909 5 GTSLTYRKLLTGAIALARKLA-KMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENF---ATTLQQVIARTDVRhvvVASLGDLLGPVRGmavnfvARRVKKLvppyrLPGATPFRTALRDGARQPFQPaal 202
Cdd:cd05909 84 TSKQFiekLKLHHLFDVEYDAR---IVYLEDLRAKISK------ADKCKAF-----LAGKFPPKWLLRIFGVAPVQP--- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 nhDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpALRKAPQIDALVTVCALPLYHILALNACMLMGARWGAMN 282
Cdd:cd05909 147 --DDPAVILFTSGSEGLPKGVVLSHKNLLANVEQ-------ITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKV 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLN--HPGfaalDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGY 360
Cdd:cd05909 218 VFHPNPLDYKKIPELIYDKKATILLGTPTFLRGYARaaHPE----DFSSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGY 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 361 GLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFK 439
Cdd:cd05909 294 GTTECSPVISVNTPQSPNKEGTVGRPLPGMEVKIVSvETHEEVPIGEGGLLLVRGPNVMLGYLNEPELTSFAFG-DGWYD 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 440 SGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAH-PGVIECACVGVPDSNSGEAVKLFVVrrDPALTTEALM 518
Cdd:cd05909 373 TGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLTT--TTDTDPSSLN 450
|
490 500 510
....*....|....*....|....*....|....*...
gi 979216869 519 AYCKK-EFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05909 451 DILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKA 488
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
46-555 |
7.88e-61 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 210.47 E-value: 7.88e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWL-QLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD-SGAEA 123
Cdd:PLN02574 64 GFSISYSELQPLVKSMAAGLyHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDcSVGLA 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 124 IVVLENfattlqqviartdvrhvvVASLGDLLGPVRGMAVNFVARRVKKLVPPYRlpgatpfrtALRDGARQPFQPAALN 203
Cdd:PLN02574 144 FTSPEN------------------VEKLSPLGVPVIGVPENYDFDSKRIEFPKFY---------ELIKEDFDFVPKPVIK 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAFLQYTGGTTGVSKGATLTHRNVIANIlQNDAWSQPALRKAPQIDAlVTVCALPLYHILALnACMLMGARWGAMNL 283
Cdd:PLN02574 197 QDDVAAIMYSSGTTGASKGVVLTHRNLIAMV-ELFVRFEASQYEYPGSDN-VYLAALPMFHIYGL-SLFVVGLLSLGSTI 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHP-GFAALDFSGLRITLGG-----GMAVQEVVSTrwmkTTGCPIL 357
Cdd:PLN02574 274 VVMRRFDASDMVKVIDRFKVTHFPVVPPILMALTKKAkGVCGEVLKSLKQVSCGaaplsGKFIQDFVQT----LPHVDFI 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPSVT--LSPPTLGAFTgTIGLPVPSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTP 434
Cdd:PLN02574 350 QGYGMTESTAVGTrgFNTEKLSKYS-SVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDK 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 435 DGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR-DPALT 513
Cdd:PLN02574 429 DGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRqGSTLS 508
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 979216869 514 TEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PLN02574 509 QEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKR 550
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
60-554 |
3.74e-60 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 206.14 E-value: 3.74e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 60 HLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGftvvnvnplytprelehqlrdsGAEAIVVLEnfattLQQVIA 139
Cdd:cd05922 5 AAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAG----------------------GRLGLVFVP-----LNPTLK 57
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 140 RTDVRHVVVASLGDLLGPVRGMAvnfvaRRVKKLVPPYRLPGaTPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGV 219
Cdd:cd05922 58 ESVLRYLVADAGGRIVLADAGAA-----DRLRDALPASPDPG-TVLDADGIRAARASAPAHEVSHEDLALLLYTSGSTGS 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 220 SKGATLTHRNVIANilqndawsQPALRKAPQIDAL-VTVCALPL---YHILALNACMLMGARwgamnlLIPNPRDV--DG 293
Cdd:cd05922 132 PKLVRLSHQNLLAN--------ARSIAEYLGITADdRALTVLPLsydYGLSVLNTHLLRGAT------LVLTNDGVldDA 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 294 FVKELSRYRVNFFPGVNTLFnALLNHPGFAALDFSGLR-ITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLS 372
Cdd:cd05922 198 FWEDLREHGATGLAGVPSTY-AMLTRLGFDPAKLPSLRyLTQAGGRLPQETIARLRELLPGAQVYVMYGQTEATRRMTYL 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 373 PPTL-GAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGF 451
Cdd:cd05922 277 PPERiLEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRDEDGF 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 452 VRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSnSGEAVKLFVVrRDPALTTEALMAYCKKEFTGYKKP 531
Cdd:cd05922 357 LFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDP-LGEKLALFVT-APDKIDPKDVLRSLAERLPPYKVP 434
|
490 500
....*....|....*....|...
gi 979216869 532 KYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05922 435 ATVRVVDELPLTASGKVDYAALR 457
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
46-554 |
5.68e-60 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 205.02 E-value: 5.68e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLG-AWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDsGAEAI 124
Cdd:cd05958 8 EREWTYRDLLALANRIAnVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDK-ARITV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 VVLEnfattlqqviartdvrHVVVASlgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnh 204
Cdd:cd05958 87 ALCA----------------HALTAS------------------------------------------------------ 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKAPQiDalVTVCALPLYHILALNACMLMGARWGAMNLL 284
Cdd:cd05958 97 DDICILAFTSGTTGAPKATMHFHRDPLASA---DRYAVNVLRLRED-D--RFVGSPPLAFTFGLGGVLLFPFGVGASGVL 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 285 IPNpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSE 364
Cdd:cd05958 171 LEE-ATPDLLLSAIARYKPTVLFTAPTAYRAMLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTE 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 365 TSpSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPqvmAGYWNRPDETAKVMTPDGFFKSGDVG 444
Cdd:cd05958 250 MF-HIFISARPGDARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGP---TGCRYLADKRQRTYVQGGWNITGDTY 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 445 VMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR-----DPALtTEALMA 519
Cdd:cd05958 326 SRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRpgvipGPVL-ARELQD 404
|
490 500 510
....*....|....*....|....*....|....*
gi 979216869 520 YCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05958 405 HAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
50-555 |
7.56e-59 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 202.28 E-value: 7.56e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVlen 129
Cdd:cd05971 8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 fattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrDGArqpfqpaalnhDDVAF 209
Cdd:cd05971 85 -------------------------------------------------------------DGS-----------DDPAL 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 210 LQYTGGTTGVSKGATLTHRNVIANiLQNDawsQPALRKAPQ-------------IDALVTVCALPLYHILALNAC----- 271
Cdd:cd05971 93 IIYTSGTTGPPKGALHAHRVLLGH-LPGV---QFPFNLFPRdgdlywtpadwawIGGLLDVLLPSLYFGVPVLAHrmtkf 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 272 -----MLMGARWGAMNLLIPNP--RDVDGFVKELSRYRVNffpgvntlfnallnhpgfaaldfsgLRITLGGGMAVQEVv 344
Cdd:cd05971 169 dpkaaLDLMSRYGVTTAFLPPTalKMMRQQGEQLKHAQVK-------------------------LRAIATGGESLGEE- 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 345 STRWMKTT-GCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQ--VMAGY 421
Cdd:cd05971 223 LLGWAREQfGVEVNEFYGQTECNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDpvAFLGY 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 422 WNRPDETAKVMTPDgFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAV 501
Cdd:cd05971 303 WNNPSATEKKMAGD-WLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIV 381
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 502 KLFVVRRDPALTTEALMA----YCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05971 382 KAFVVLNPGETPSDALAReiqeLVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
24-553 |
1.22e-58 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 202.56 E-value: 1.22e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNV 103
Cdd:cd05920 16 PLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 104 NPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARtDVRHvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgat 183
Cdd:cd05920 96 LPSHRRSELSAFCAHAEAVAYIVPDRHAGFDHRALAR-ELAE-------------------------------------- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 184 pfrtalrdgarqpfqpaalNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqpalRKAPQIDAL----VTVCA 259
Cdd:cd05920 137 -------------------SIPEVALFLLSGGTTGTPKLIPRTHNDYAYNV-----------RASAEVCGLdqdtVYLAV 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 260 LPLYHILALNACMLMGARW-GAMNLLIPNPRDVDGFvKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGM 338
Cdd:cd05920 187 LPAAHNFPLACPGVLGTLLaGGRVVLAPDPSPDAAF-PLIEREGVTVTALVPALVSLWLDAAASRRADLSSLRLLQVGGA 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 339 AVQEVVSTRWMKTTGCPILEGYGLSETSPSVTL--SPPTLGAftGTIGLPV-PSTDIALLGENDEPVAQGQPGEIAIRGP 415
Cdd:cd05920 266 RLSPALARRVPPVLGCTLQQVFGMAEGLLNYTRldDPDEVII--HTQGRPMsPDDEIRVVDEEGNPVPPGEEGELLTRGP 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 416 QVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDS 495
Cdd:cd05920 344 YTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDE 423
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 496 NSGEAVKLFVVRRDPALTTEALMAYCKK-EFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05920 424 LLGERSCAFVVLRDPPPSAAQLRRFLRErGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
32-553 |
1.44e-58 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 202.74 E-value: 1.44e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 32 SFATFAARNAYACM----GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLY 107
Cdd:cd05923 8 RRAASRAPDACAIAdparGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 108 TPRELEhqlrdsgaeaivvlenfattlqQVIARTDVRHVVVAslgDLLGPVRGMAVNFVarRVKKLVPPYRLPGATPFRT 187
Cdd:cd05923 88 KAAELA----------------------ELIERGEMTAAVIA---VDAQVMDAIFQSGV--RVLALSDLVGLGEPESAGP 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 188 ALRDGARQPFQPAalnhddvaFLQYTGGTTGVSKGATLTHRNVIANILQNDawSQPALRKApqiDALVTVCALPLYHILA 267
Cdd:cd05923 141 LIEDPPREPEQPA--------FVFYTSGTTGLPKGAVIPQRAAESRVLFMS--TQAGLRHG---RHNVVLGLMPLYHVIG 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 268 LNACMLMGArwgAMNLLIPNPRDVD--GFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVS 345
Cdd:cd05923 208 FFAVLVAAL---ALDGTYVVVEEFDpaDALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLSSLRHVTFAGATMPDAVL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 346 TRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAfTGTIGLPVPSTDIALLGENDEPVAQGQPGEI--AIRGPQVMAGYWN 423
Cdd:cd05923 285 ERVNQHLPGEKVNIYGTTEAMNSLYMRDARTGT-EMRPGFFSEVRIVRIGGSPDEALANGEEGELivAAAADAAFTGYLN 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 424 RPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKL 503
Cdd:cd05923 364 QPEATAKKLQ-DGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTA 442
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 504 FVVRRDPALTTEALMAYCK-KEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05923 443 CVVPREGTLSADELDQFCRaSELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
46-555 |
3.99e-58 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 202.23 E-value: 3.99e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK13391 22 GEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfATTLQQVIARTDVRHVvvaslgdllgP-VRGMAVnfvarrvkkLVPPYRLPGATPFRTALRDgarqpfQPAALNH 204
Cdd:PRK13391 102 -----TSAAKLDVARALLKQC----------PgVRHRLV---------LDGDGELEGFVGYAEAVAG------LPATPIA 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDV--AFLQYTGGTTGVSKG--ATLTHRNV-----IANILQNdAWsqpalrkapQIDA-LVTVCALPLYHILALNACMLm 274
Cdd:PRK13391 152 DESlgTDMLYSSGTTGRPKGikRPLPEQPPdtplpLTAFLQR-LW---------GFRSdMVYLSPAPLYHSAPQRAVML- 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 GARWGAMNLLIPNpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFA--ALDFSGLRITLGGGMAVQEVVSTRWMKTT 352
Cdd:PRK13391 221 VIRLGGTVIVMEH-FDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVrdKYDLSSLEVAIHAAAPCPPQVKEQMIDWW 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 353 GCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPStDIALLGENDEPVAQGQPGEIAIRGPQvMAGYWNRPDETAKVM 432
Cdd:PRK13391 300 GPIIHEYYAATEGLGFTACDSEEWLAHPGTVGRAMFG-DLHILDDDGAELPPGEPGTIWFEGGR-PFEYLNDPAKTAEAR 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TPDG-FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA 511
Cdd:PRK13391 378 HPDGtWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGV 457
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 979216869 512 LTTEA----LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK13391 458 DPGPAlaaeLIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRD 505
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
24-561 |
1.38e-57 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 201.53 E-value: 1.38e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNV 103
Cdd:PRK06155 22 TLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 104 NPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIAR-TDVRHVVvaslgdLLGPVRGMAVnfvarrvkklvppyrlpgA 182
Cdd:PRK06155 102 NTALRGPQLEHILRNSGARLLVVEAALLAALEAADPGdLPLPAVW------LLDAPASVSV------------------P 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 183 TPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHrnviANILQNDAWSQPALRKAPQiDALVTvcALPL 262
Cdd:PRK06155 158 AGWSTAPLPPLDAPAPAAAVQPGDTAAILYTSGTTGPSKGVCCPH----AQFYWWGRNSAEDLEIGAD-DVLYT--TLPL 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 263 YHILALNA---CMLMGARwgamnlLIPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGM 338
Cdd:PRK06155 231 FHTNALNAffqALLAGAT------YVLEPRfSASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRVALGPGV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 339 AVQEVVSTRwmKTTGCPILEGYGLSETSPSVTLSPPTLGAftGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQ-- 416
Cdd:PRK06155 305 PAALHAAFR--ERFGVDLLDGYGSTETNFVIAVTHGSQRP--GSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEpf 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 417 -VMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDS 495
Cdd:PRK06155 381 aFATGYFGMPEKTVEAWR-NLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSE 459
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 496 NSGEAVKLFVVRRD-PALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRdEHGQTA 561
Cdd:PRK06155 460 LGEDEVMAAVVLRDgTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLR-EQGVTA 525
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
46-553 |
1.82e-56 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 195.82 E-value: 1.82e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05930 10 DQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYILEDSGAKLVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalNHD 205
Cdd:cd05930 90 T----------------------------------------------------------------------------DPD 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANILqndawsqpALRKAPQIDALVTVCALPLY----HILALNACMLMGARwgam 281
Cdd:cd05930 94 DLAYVIYTSGSTGKPKGVMVEHRGLVNLLL--------WMQEAYPLTPGDRVLQFTSFsfdvSVWEIFGALLAGAT---- 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 nLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAalDFSGLRITLGGGMAVQEVVSTRWMKT-TGCPIL 357
Cdd:cd05930 162 -LVVLPEevrKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA--ALPSLRLVLVGGEALPPDLVRRWRELlPGARLV 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPSVT---LSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTP 434
Cdd:cd05930 239 NLYGPTEATVDATyyrVPPDDEEDGRVPIGRPIPNTRVYVLDENLRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVP 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 435 DGFF------KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV-R 507
Cdd:cd05930 319 NPFGpgermyRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVpD 398
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 979216869 508 RDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05930 399 EGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
12-555 |
3.63e-56 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 197.84 E-value: 3.63e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 12 GVPATVDPDRYPSLAHLLDE--SFATFAARNAYACMGR--------TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMP 81
Cdd:PRK07788 28 GAVDLERPDNGLRLAADIRRygPFAGLVAHAARRAPDRaalidergTLTYAELDEQSNALARGLLALGVRAGDGVAVLAR 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 82 NVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGM 161
Cdd:PRK07788 108 NHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVAAREGVKALVYDDEFTDLLSALPPDLGRLRAWGGNPDDDEPSGSTD 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 162 AV--NFVARRVKKLVPPYRLPGAtpfrtalrdgarqpfqpaalnhddvaFLQYTGGTTGVSKGATLTHRN---VIANILQ 236
Cdd:PRK07788 188 ETldDLIAGSSTAPLPKPPKPGG--------------------------IVILTSGTTGTPKGAPRPEPSplaPLAGLLS 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 237 NDAWsqpalrKAPQidalVTVCALPLYH---------ILALNACMLMGARWGAMNLLipnpRDVDgfvkelsRYRVNFFP 307
Cdd:PRK07788 242 RVPF------RAGE----TTLLPAPMFHatgwahltlAMALGSTVVLRRRFDPEATL----EDIA-------KHKATALV 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 308 GVNTLFNALLNHPG--FAALDFSGLRITLGGGMAVQEVVSTRWMKTTGcPILEG-YGLSETSPSVTLSPPTLGAFTGTIG 384
Cdd:PRK07788 301 VVPVMLSRILDLGPevLAKYDTSSLKIIFVSGSALSPELATRALEAFG-PVLYNlYGSTEVAFATIATPEDLAEAPGTVG 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 385 LPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWN-RPDETAkvmtpDGFFKSGDVGVMDEHGFVRIVDRKKDMIL 463
Cdd:PRK07788 380 RPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGYTDgRDKQII-----DGLLSSGDVGYFDEDGLLFVDGRDDDMIV 454
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 464 VSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPK 542
Cdd:PRK07788 455 SGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAaLDEDAIKDYVRDNLARYKVPRDVVFLDELPR 534
|
570
....*....|...
gi 979216869 543 SNIGKILRRELRD 555
Cdd:PRK07788 535 NPTGKVLKRELRE 547
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
24-555 |
5.64e-56 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 196.98 E-value: 5.64e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAY--ACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVV 101
Cdd:cd17642 18 EQLHKAMKRYASVPGTIAFtdAHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVA 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 102 NVNPLYTPRELEHQLRDSGAEAIVVLENfatTLQQVI----ARTDVRHVVVASLGDLLGPVRGMAvNFVARRVKKLVPPY 177
Cdd:cd17642 98 PTNDIYNERELDHSLNISKPTIVFCSKK---GLQKVLnvqkKLKIIKTIIILDSKEDYKGYQCLY-TFITQNLPPGFNEY 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 178 RlpgatpfrtalrdgarqpFQPAALNHD-DVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKAPQID-ALV 255
Cdd:cd17642 174 D------------------FKPPSFDRDeQVALIMNSSGSTGLPKGVQLTHKNIVARF---SHARDPIFGNQIIPDtAIL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 256 TVcaLPLYH---ILALNACMLMGARWGAMnllipnPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR 331
Cdd:cd17642 233 TV--IPFHHgfgMFTTLGYLICGFRVVLM------YKfEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDKYDLSNLH 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 332 ITLGGGMAVQEVVSTRWMKTTGCP-ILEGYGLSETSPSVTLSPPTLGAfTGTIGLPVP--------STDIALLGENdepv 402
Cdd:cd17642 305 EIASGGAPLSKEVGEAVAKRFKLPgIRQGYGLTETTSAILITPEGDDK-PGAVGKVVPffyakvvdLDTGKTLGPN---- 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 aqgQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHP 482
Cdd:cd17642 380 ---ERGELCVKGPMIMKGYVNNPEATKALIDKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHP 456
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 483 GVIECACVGVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPK-YIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd17642 457 KIFDAGVAGIPDEDAGELPAAVVVLEaGKTMTEKEVMDYVASQVSTAKRLRgGVKFVDEVPKGLTGKIDRRKIRE 531
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
29-556 |
6.08e-56 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 195.87 E-value: 6.08e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 29 LDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:PRK06145 4 LSASIAFHARRTpdraALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPIN 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAEAIVVLENFAttlqqVIARTDVRHVVVaslgdllgpvrGMAVNFVARRVkklvppyrlpgATP 184
Cdd:PRK06145 84 YRLAADEVAYILGDAGAKLLLVDEEFD-----AIVALETPKIVI-----------DAAAQADSRRL-----------AQG 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 FRTALRDGARQPfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVianilqndAWsqpalRKAPQIDAL------VTVC 258
Cdd:PRK06145 137 GLEIPPQAAVAP--------TDLVRLMYTSGTTDRPKGVMHSYGNL--------HW-----KSIDHVIALgltaseRLLV 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHIlalNACMLMG--ARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGG 336
Cdd:PRK06145 196 VGPLYHV---GAFDLPGiaVLWVGGTLRIHREFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGG 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVVSTRWMKT-TGCPILEGYGLSETSPSVTLSPPTLG-AFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG 414
Cdd:PRK06145 273 GEKTPESRIRDFTRVfTRARYIDAYGLTETCSGDTLMEAGREiEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRG 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 415 PQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPD 494
Cdd:PRK06145 353 PKVTKGYWKDPEKTAEAFY-GDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHD 431
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 495 SNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK06145 432 DRWGERITAVVVLNPGAtLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLRDE 494
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
204-554 |
1.33e-55 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 191.15 E-value: 1.33e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAFLQYTGGTTGVSKGATLTHRNVIANilqndAWSQPALRKAPQIDALVtvCALPLYHILA-LNACMLMGARWGAMN 282
Cdd:cd05944 1 SDDVAAYFHTGGTTGTPKLAQHTHSNEVYN-----AWMLALNSLFDPDDVLL--CGLPLFHVNGsVVTLLTPLASGAHVV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLIP----NPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAalDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILE 358
Cdd:cd05944 74 LAGPagyrNPGLFDNFWKLVERYRITSLSTVPTVYAALLQVPVNA--DISSLRFAMSGAAPLPVELRARFEDATGLPVVE 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDE-----PVAQGQPGEIAIRGPQVMAGYWNrpDETAK-VM 432
Cdd:cd05944 152 GYGLTEATCLVAVNPPDGPKRPGSVGLRLPYARVRIKVLDGVgrllrDCAPDEVGEICVAGPGVFGGYLY--TEGNKnAF 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV-VRRDPA 511
Cdd:cd05944 230 VADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVqLKPGAV 309
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 979216869 512 LTTEALMAYCKKEFTGYKK-PKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05944 310 VEEEELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALR 353
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
47-554 |
1.35e-55 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 195.29 E-value: 1.35e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAEL-DTYSRHLGAWLQLrGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK08008 36 RRYSYLELnEEINRTANLFYSL-GIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQASLLV 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATTLQQVIARTD--VRHVVVASLGDllgPVRGMAVNFVARRVKklvppyrlpgatpfrtalrdgarqpfQPAALN 203
Cdd:PRK08008 115 TSAQFYPMYRQIQQEDAtpLRHICLTRVAL---PADDGVSSFTQLKAQ--------------------------QPATLC 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 H------DDVAFLQYTGGTTGVSKGATLTHRNVI-ANILQndAWsQPALRKApqiDALVTVcaLPLYHI-LALNACMLM- 274
Cdd:PRK08008 166 YapplstDDTAEILFTSGTTSRPKGVVITHYNLRfAGYYS--AW-QCALRDD---DVYLTV--MPAFHIdCQCTAAMAAf 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 --GARwgamnLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR-ITLGGGMAVQE--VVSTRWm 349
Cdd:PRK08008 238 saGAT-----FVLLEKYSARAFWGQVCKYRATITECIPMMIRTLMVQPPSANDRQHCLReVMFYLNLSDQEkdAFEERF- 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 kttGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG---PQVMAGYWNRPD 426
Cdd:PRK08008 312 ---GVRLLTSYGMTETIVGIIGDRPGDKRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGvpgKTIFKEYYLDPK 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 427 ETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV 506
Cdd:PRK08008 389 ATAKVLEADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVV 468
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 979216869 507 RRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK08008 469 LNEGEtLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
47-555 |
1.43e-55 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 195.79 E-value: 1.43e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRdsgaeaivv 126
Cdd:cd05970 46 RIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIE--------- 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlqqviaRTDVRHVVVASLGDLLGPVRGMAVNFVARRVKKLVPPYRLPGATPFRTALRDGA---RQPFQPAALN 203
Cdd:cd05970 117 -------------SADIKMIVAIAEDNIPEEIEKAAPECPSKPKLVWVGDPVPEGWIDFRKLIKNASpdfERPTANSYPC 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQpaLRKapqiDALvtvcalplyHiLALNACMLMGARWGAM-- 281
Cdd:cd05970 184 GEDILLVYFSSGTTGMPKMVEHDFTYPLGHIVTAKYWQN--VRE----GGL---------H-LTVADTGWGKAVWGKIyg 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 ------NLLIpnpRDVDGFVKE-----LSRYRVNFFPGVNTLFNALLnHPGFAALDFSGLRITLGGGMAVQEVVSTRWMK 350
Cdd:cd05970 248 qwiagaAVFV---YDYDKFDPKallekLSKYGVTTFCAPPTIYRFLI-REDLSRYDLSSLRYCTTAGEALNPEVFNTFKE 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 351 TTGCPILEGYGLSETSPSVTlSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQ-----VMAGYWNRP 425
Cdd:cd05970 324 KTGIKLMEGFGQTETTLTIA-TFPWMEPKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSKgkpvgLFGGYYKDA 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 426 DETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV 505
Cdd:cd05970 403 EKTAEVWH-DGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATI 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 506 V-----RRDPALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05970 482 VlakgyEPSEELKKE-LQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIRE 535
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
28-555 |
1.39e-53 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 189.05 E-value: 1.39e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 28 LLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNV-----LQYPVAVAAALragftVVN 102
Cdd:cd12118 9 FLERAAAVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTpamyeLHFGVPMAGAV-----LNA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 103 VNPLYTPRELEHQLRDSGAEAIVVLENFATtlQQVIARTDvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlPGA 182
Cdd:cd12118 84 LNTRLDAEEIAFILRHSEAKVLFVDREFEY--EDLLAEGD-------------------------------------PDF 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 183 TPfrtalrdgarqpfQPAALNHDDVAfLQYTGGTTGVSKGATLTHR----NVIANILQNDAWSQPalrkapqidalVTVC 258
Cdd:cd12118 125 EW-------------IPPADEWDPIA-LNYTSGTTGRPKGVVYHHRgaylNALANILEWEMKQHP-----------VYLW 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHilALNACML--MGARwGAMNLLIpnpRDVD--GFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITL 334
Cdd:cd12118 180 TLPMFH--CNGWCFPwtVAAV-GGTNVCL---RKVDakAIYDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPHRVHVM 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 335 GGGMAVQEVVSTRwMKTTGCPILEGYGLSETSPSVT----------LSPPTLGAFTGTIGLPVPSTDiALLGENDE---P 401
Cdd:cd12118 254 TAGAPPPAAVLAK-MEELGFDVTHVYGLTETYGPATvcawkpewdeLPTEERARLKARQGVRYVGLE-EVDVLDPEtmkP 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 402 V-AQGQP-GEIAIRGPQVMAGYWNRPDETAKVmTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVA 479
Cdd:cd12118 332 VpRDGKTiGEIVFRGNIVMKGYLKNPEATAEA-FRGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLY 410
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 480 AHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEA-LMAYCKKEFTGYKKPKYIEFRDsLPKSNIGKILRRELRD 555
Cdd:cd12118 411 KHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEEeIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVLRD 486
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
24-547 |
1.53e-53 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 190.10 E-value: 1.53e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNV 103
Cdd:PRK07798 4 NIADLFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 104 NPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTD-VRHVVVASLGDLLGPvrgmavnfvarrvkklvppyrLPGA 182
Cdd:PRK07798 84 NYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLPRLPkLRTLVVVEDGSGNDL---------------------LPGA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 183 TPFRTALRDGA--RQPFQPAAlnhDDVAFLqYTGGTTGVSKG----------ATLTHRNVIANILQNDAWSQpaLRKAPQ 250
Cdd:PRK07798 143 VDYEDALAAGSpeRDFGERSP---DDLYLL-YTGGTTGMPKGvmwrqedifrVLLGGRDFATGEPIEDEEEL--AKRAAA 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 251 IDALVTVCALPLYHILALNA---CMLMGARwgamNLLIPNPR-DVDGFVKELSRYRVN--FFPG---VNTLFNALLNHPG 321
Cdd:PRK07798 217 GPGMRRFPAPPLMHGAGQWAafaALFSGQT----VVLLPDVRfDADEVWRTIEREKVNviTIVGdamARPLLDALEARGP 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 322 FaalDFSGLRITLGGGMAVQEVVSTRWMKTTgcP---ILEGYGLSET----SPSVTLSPPTLGAFTGTIGlpvPSTdiAL 394
Cdd:PRK07798 293 Y---DLSSLFAIASGGALFSPSVKEALLELL--PnvvLTDSIGSSETgfggSGTVAKGAVHTGGPRFTIG---PRT--VV 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 395 LGENDEPVA--QGQPGEIAIRGPqVMAGYWNRPDETAKVM-TPDG--FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNV 469
Cdd:PRK07798 363 LDEDGNPVEpgSGEIGWIARRGH-IPLGYYKDPEKTAETFpTIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGGEKV 441
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 470 YPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:PRK07798 442 FPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGArPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
59-555 |
4.54e-53 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 187.58 E-value: 4.54e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 59 RHLGAWLQL----RGLRKGARVAVMMPnvlqypvaVAAALRAGFTVVNVNplYTPRELEHQLRDSGAEAIVVLENFATTL 134
Cdd:cd05929 12 FHQRRLLLLdvysIALNRNARAAAAEG--------VWIADGVYIYLINSI--LTVFAAAAAWKCGACPAYKSSRAPRAEA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 135 QQVI-ARTDVRHVVVASLGDLLGPVRGMAVNfVARRVKKLVPPYRLPGAtpfrtalrdgarqpfqpaalnhddvafLQYT 213
Cdd:cd05929 82 CAIIeIKAAALVCGLFTGGGALDGLEDYEAA-EGGSPETPIEDEAAGWK---------------------------MLYS 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 214 GGTTGVSKGatlTHRNVIANILQNDAWSQPALRKAPQIDAlVTVCALPLYHILALNACMlMGARWGAMNLLIPNpRDVDG 293
Cdd:cd05929 134 GGTTGRPKG---IKRGLPGGPPDNDTLMAAALGFGPGADS-VYLSPAPLYHAAPFRWSM-TALFMGGTLVLMEK-FDPEE 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 294 FVKELSRYRVNFFPGVNTLFNALLNHPG--FAALDFSGLR--ITLGGGMAVQevVSTRWMKTTGCPILEGYGLSETSPSV 369
Cdd:cd05929 208 FLRLIERYRVTFAQFVPTMFVRLLKLPEavRNAYDLSSLKrvIHAAAPCPPW--VKEQWIDWGGPIIWEYYGGTEGQGLT 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 370 TLSPPTLGAFTGTIGLPVPStDIALLGENDEPVAQGQPGEIAIRGPQVMAgYWNRPDETAKVMTPDGFFKSGDVGVMDEH 449
Cdd:cd05929 286 IINGEEWLTHPGSVGRAVLG-KVHILDEDGNEVPPGEIGEVYFANGPGFE-YTNDPEKTAAARNEGGWSTLGDVGYLDED 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 450 GFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV-----VRRDPALtTEALMAYCKKE 524
Cdd:cd05929 364 GYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVqpapgADAGTAL-AEELIAFLRDR 442
|
490 500 510
....*....|....*....|....*....|.
gi 979216869 525 FTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05929 443 LSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
46-550 |
9.79e-53 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 186.49 E-value: 9.79e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05914 5 GEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIF 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnhD 205
Cdd:cd05914 85 VSDE---------------------------------------------------------------------------D 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKAPQidalVTVCALPLYHILALNACMLMGARWGAMNLL- 284
Cdd:cd05914 90 DVALINYTSGTTGNSKGVMLTYRNIVSNV---DGVKEVVLLGKGD----KILSILPLHHIYPLTFTLLLPLLNGAHVVFl 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 285 --IPNPRdvdgfVKELSRYRVNFFPGVNTLF-------NALLN---------------------HPGFAAL--DFSG-LR 331
Cdd:cd05914 163 dkIPSAK-----IIALAFAQVTPTLGVPVPLviekifkMDIIPkltlkkfkfklakkinnrkirKLAFKKVheAFGGnIK 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 332 ITLGGGMAV-QEVvsTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTiGLPVPSTDIALlgenDEPVAQGQPGEI 410
Cdd:cd05914 238 EFVIGGAKInPDV--EEFLRTIGFPYTIGYGMTETAPIISYSPPNRIRLGSA-GKVIDGVEVRI----DSPDPATGEGEI 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 411 AIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILV-SGFNVYPNEIESVVAAHPGVIEcAC 489
Cdd:cd05914 311 IVRGPNVMKGYYKNPEATAEAFDKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNMPFVLE-SL 389
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 979216869 490 VGVPDSNS---------GEAVKLFVVRRDPALTTEALMAYCKKEFTGYKK-PKYIEFRDSLPKSNIGKILR 550
Cdd:cd05914 390 VVVQEKKLvalayidpdFLDVKALKQRNIIDAIKWEVRDKVNQKVPNYKKiSKVKIVKEEFEKTPKGKIKR 460
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
47-555 |
2.20e-52 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 187.45 E-value: 2.20e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRkGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYTPRELEHQLRdsgaeaivv 126
Cdd:cd05931 23 ETLTYAELDRRARAIAARLQAVGKP-GDRVLLLAPPGLDFVAAFLGCLYAGAIAV---PLPPPTPGRHAER--------- 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattLQQVIArtDVRHVVVASLGDLLGPVRGMAVNfvarrvkklvPPYRLPGATPFRTALRDGARQPFQPAALNHDD 206
Cdd:cd05931 90 -------LAAILA--DAGPRVVLTTAAALAAVRAFAAS----------RPAAGTPRLLVVDLLPDTSAADWPPPSPDPDD 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 VAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpaLRKAPQIDALVTVCA-LPLYHILALNACMLMGARWGAMNLLI 285
Cdd:cd05931 151 IAYLQYTSGSTGTPKGVVVTHRNLLANVRQ--------IRRAYGLDPGDVVVSwLPLYHDMGLIGGLLTPLYSGGPSVLM 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 286 PnPRDvdgFV-------KELSRYRVNFFPGvntlfnallnhPGFA--------------ALDFSGLRITLGGGMAVQEVV 344
Cdd:cd05931 223 S-PAA---FLrrplrwlRLISRYRATISAA-----------PNFAydlcvrrvrdedleGLDLSSWRVALNGAEPVRPAT 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 345 STRWMKTTG---------CPileGYGLSETSPSVTLSP----PTLGAFTGTI---------------------GLPVPST 390
Cdd:cd05931 288 LRRFAEAFApfgfrpeafRP---SYGLAEATLFVSGGPpgtgPVVLRVDRDAlagravavaaddpaarelvscGRPLPDQ 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 391 DIALL-GENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTP------DGFFKSGDVGVMDEHGFVrIVDRKKDMIL 463
Cdd:cd05931 365 EVRIVdPETGRELPDGEVGEIWVRGPSVASGYWGRPEATAETFGAlaatdeGGWLRTGDLGFLHDGELY-ITGRLKDLII 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 464 VSGFNVYPNEIESVVAAHPGVIE---CACVGVPDSNSGEAVKLFVVRR-----DPALTTEALMAYCKKEFtGYkKPKYIE 535
Cdd:cd05931 444 VRGRNHYPQDIEATAEEAHPALRpgcVAAFSVPDDGEERLVVVAEVERgadpaDLAAIAAAIRAAVAREH-GV-APADVV 521
|
570 580
....*....|....*....|..
gi 979216869 536 F--RDSLPKSNIGKILRRELRD 555
Cdd:cd05931 522 LvrPGSIPRTSSGKIQRRACRA 543
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
47-556 |
2.74e-52 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 187.18 E-value: 2.74e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGfTVVN-VNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK13295 54 RRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIG-AVLNpLMPIFRERELSFMLKHAESKVLV 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENF--------ATTLQQVIArtDVRHVVVASLGDllgpvrgmAVNFVARrvkkLVPPYR--LPGATPFRTALRDGArq 195
Cdd:PRK13295 133 VPKTFrgfdhaamARRLRPELP--ALRHVVVVGGDG--------ADSFEAL----LITPAWeqEPDAPAILARLRPGP-- 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 196 pfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqpalrkAPQIDAL------VTVCALPLYHILALN 269
Cdd:PRK13295 197 ---------DDVTQLIYTSGTTGEPKGVMHTANTLMANI-------------VPYAERLglgaddVILMASPMAHQTGFM 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 270 ACMLMGARWGAMNLLiPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWM 349
Cdd:PRK13295 255 YGLMMPVMLGATAVL-QDIWDPARAAELIRTEGVTFTMASTPFLTDLTRAVKESGRPVSSLRTFLCAGAPIPGALVERAR 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPILEGYGLSETSpSVTLSPP--TLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDE 427
Cdd:PRK13295 334 AALGAKIVSAWGMTENG-AVTLTKLddPDERASTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQVRGCSNFGGYLKRPQL 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 428 TAkvMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVR 507
Cdd:PRK13295 413 NG--TDADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVP 490
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 508 RDPALTTEALMayckKEFTGYKK------PKYIEFRDSLPKSNIGKI----LRRELRDE 556
Cdd:PRK13295 491 RPGQSLDFEEM----VEFLKAQKvakqyiPERLVVRDALPRTPSGKIqkfrLREMLRGE 545
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
50-556 |
2.81e-52 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 186.90 E-value: 2.81e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWL-QLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLE 128
Cdd:cd05928 43 SFRELGSLSRKAANVLsGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSD 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 NFATTLQQVIARTdvrhvvvASLgdllgpvrgmavnfvarRVKKLVPPYRLPGATPFRTALRDgARQPFQPAALNHDDVA 208
Cdd:cd05928 123 ELAPEVDSVASEC-------PSL-----------------KTKLLVSEKSRDGWLNFKELLNE-ASTEHHCVETGSQEPM 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGA-------------------TLTHRNVIANIlQNDAWSQPALRK--APQID-ALVTVCALPLYhil 266
Cdd:cd05928 178 AIYFTSGTTGSPKMAehshsslglglkvngrywlDLTASDIMWNT-SDTGWIKSAWSSlfEPWIQgACVFVHHLPRF--- 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 267 alnacmlmgarwgamnllipnprDVDGFVKELSRYRVNFFPGVNTLFNALLNHpGFAALDFSGLRITLGGGMAVQEVVST 346
Cdd:cd05928 254 -----------------------DPLVILKTLSSYPITTFCGAPTVYRMLVQQ-DLSSYKFPSLQHCVTGGEPLNPEVLE 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 347 RWMKTTGCPILEGYGLSETSpSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIR-GPQ----VMAGY 421
Cdd:cd05928 310 KWKAQTGLDIYEGYGQTETG-LICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRvKPIrpfgLFSGY 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 422 WNRPDETAKVMTPDgFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAV 501
Cdd:cd05928 389 VDNPEKTAATIRGD-FYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVV 467
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 979216869 502 KLFVV------RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:cd05928 468 KAFVVlapqflSHDPEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRDK 528
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
50-490 |
2.96e-51 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 180.92 E-value: 2.96e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQ-LRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLE 128
Cdd:TIGR01733 1 TYRELDERANRLARHLRaAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 NFATTL-QQVIARTDVRHVVVASLGDllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgARQPFQPAALNH-DD 206
Cdd:TIGR01733 81 ALASRLaGLVLPVILLDPLELAALDD---------------------------------------APAPPPPDAPSGpDD 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 VAFLQYTGGTTGVSKGATLTHRNVIANIL---------QNDAWSQPAlrkAPQIDALVTVCALPLYHilalnacmlmGAR 277
Cdd:TIGR01733 122 LAYVIYTSGSTGRPKGVVVTHRSLVNLLAwlarrygldPDDRVLQFA---SLSFDASVEEIFGALLA----------GAT 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 278 wgamnLLIPNPRDVDGFVKELSR----YRVNFFPGVNTLFNALLNHpgfAALDFSGLRITLGGGMAVQEVVSTRWM-KTT 352
Cdd:TIGR01733 189 -----LVVPPEDEERDDAALLAAliaeHPVTVLNLTPSLLALLAAA---LPPALASLRLVILGGEALTPALVDRWRaRGP 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 353 GCPILEGYGLSETSPSVTL----SPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDET 428
Cdd:TIGR01733 261 GARLINLYGPTETTVWSTAtlvdPDDAPRESPVPIGRPLANTRLYVLDDDLRPVPVGVVGELYIGGPGVARGYLNRPELT 340
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 429 AKVMTPDGF--------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGViECACV 490
Cdd:TIGR01733 341 AERFVPDPFaggdgarlYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGV-REAVV 409
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
39-553 |
4.29e-51 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 181.68 E-value: 4.29e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 39 RNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD 118
Cdd:cd05945 7 RPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIREILDA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 119 SGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrDGarqpfq 198
Cdd:cd05945 87 AKPALLIA----------------------------------------------------------------DG------ 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 paalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndAWSQPALRKAPQIDALVT------VCALPLYHILALNACm 272
Cdd:cd05945 97 ------DDNAYIIFTSGSTGRPKGVQISHDNLVSFT----NWMLSDFPLGPGDVFLNQapfsfdLSVMDLYPALASGAT- 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 273 lmgarwgamnlLIPNPRDVDGFVKELSRY----RVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRW 348
Cdd:cd05945 166 -----------LVPVPRDATADPKQLFRFlaehGITVWVSTPSFAAMCLLSPTFTPESLPSLRHFLFCGEVLPHKTARAL 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 349 MKTT-GCPILEGYGLSETSPSVT---LSPPTLGAFTG-TIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWN 423
Cdd:cd05945 235 QQRFpDARIYNTYGPTEATVAVTyieVTPEVLDGYDRlPIGYAKPGAKLVILDEDGRPVPPGEKGELVISGPSVSKGYLN 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 424 RPDETAKVMTPD---GFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEA 500
Cdd:cd05945 315 NPEKTAAAFFPDegqRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTE 394
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 501 VKLFVVRR--DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd05945 395 LIAFVVPKpgAEAGLTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
14-555 |
7.13e-50 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 180.32 E-value: 7.13e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 14 PATVDPDRYPSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAA 93
Cdd:PRK06164 1 TPHDAAPRADTLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLAC 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 94 LRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFAttlqqviaRTDVRHVVVASLGDLLGPVRGMAVnfVARRVKKL 173
Cdd:PRK06164 81 ARLGATVIAVNTRYRSHEVAHILGRGRARWLVVWPGFK--------GIDFAAILAAVPPDALPPLRAIAV--VDDAADAT 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 174 vpPYRLPGATPFRTALRDGARQP-FQPAALNHDDVAFLQYTGGTTGVSK------GATLTHRNVIANILQNDAWSqpalr 246
Cdd:PRK06164 151 --PAPAPGARVQLFALPDPAPPAaAGERAADPDAGALLFTTSGTTSGPKlvlhrqATLLRHARAIARAYGYDPGA----- 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 247 kapqidalVTVCALPLYHILALNacMLMGARWGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAAlD 326
Cdd:PRK06164 224 --------VLLAALPFCGVFGFS--TLLGALAGGAPLVCEPVFDAARTARALRRHRVTHTFGNDEMLRRILDTAGERA-D 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 FSGLRI----TLGGGMAvqEVVStrWMKTTGCPILEGYGLSETSPSVTLSPPTLgAFTGTI---GLPV-PSTDI-ALLGE 397
Cdd:PRK06164 293 FPSARLfgfaSFAPALG--ELAA--LARARGVPLTGLYGSSEVQALVALQPATD-PVSVRIeggGRPAsPEARVrARDPQ 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 398 NDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESV 477
Cdd:PRK06164 368 DGALLPDGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHA 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 478 VAAHPGVIECACVGVPDSNSGEAVKlFVVRRDPALTTEA-LMAYCKKEFTGYKKPKYIEFRDSLP---KSNIGKILRREL 553
Cdd:PRK06164 448 LEALPGVAAAQVVGATRDGKTVPVA-FVIPTDGASPDEAgLMAACREALAGFKVPARVQVVEAFPvteSANGAKIQKHRL 526
|
..
gi 979216869 554 RD 555
Cdd:PRK06164 527 RE 528
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
46-556 |
7.27e-50 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 180.53 E-value: 7.27e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNV-----LQYPVAVAAAlragftVVN-VNPLYTPRELEHQLRDS 119
Cdd:PRK08162 41 DRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIpamveAHFGVPMAGA------VLNtLNTRLDAASIAFMLRHG 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 120 GAEAIVVLENFATTLQQVIARTDVRHVVVASLGDllgpvrgmavnfvarrvkklvPPY---RLPGATPFRTALRDGARQ- 195
Cdd:PRK08162 115 EAKVLIVDTEFAEVAREALALLPGPKPLVIDVDD---------------------PEYpggRFIGALDYEAFLASGDPDf 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 196 PFQPAALNHDDVAfLQYTGGTTGVSKGATLTHR----NVIANILqndAWSQPalrKAPqidalVTVCALPLYHilalnaC 271
Cdd:PRK08162 174 AWTLPADEWDAIA-LNYTSGTTGNPKGVVYHHRgaylNALSNIL---AWGMP---KHP-----VYLWTLPMFH------C 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 272 ------MLMGARWGAMNLLipnpRDVD-GFVKELSR-YRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEV 343
Cdd:PRK08162 236 ngwcfpWTVAARAGTNVCL----RKVDpKLIFDLIReHGVTHYCGAPIVLSALINAPAEWRAGIDHPVHAMVAGAAPPAA 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 344 VSTRwMKTTGCPILEGYGLSET-SPSV---------TLSPPTLGAFTGTIGLPVPSTDIALLGEND--EPV-AQGQP-GE 409
Cdd:PRK08162 312 VIAK-MEEIGFDLTHVYGLTETyGPATvcawqpewdALPLDERAQLKARQGVRYPLQEGVTVLDPDtmQPVpADGETiGE 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 410 IAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECAC 489
Cdd:PRK08162 391 IMFRGNIVMKGYLKNPKATEEAFA-GGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAV 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 490 VGVPDSNSGEAVKLFVVRRDPALTTEA-LMAYCKKEFTGYKKPKYIEFrDSLPKSNIGKILRRELRDE 556
Cdd:PRK08162 470 VAKPDPKWGEVPCAFVELKDGASATEEeIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQKFVLREQ 536
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
33-553 |
9.31e-50 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 178.93 E-value: 9.31e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 33 FATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYT 108
Cdd:cd12117 3 FEEQAARTpdavAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 109 PRELEHQLRDSGAEAIVVLENFATTLQQviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvPPYRLPGATPFRTA 188
Cdd:cd12117 83 AERLAFMLADAGAKVLLTDRSLAGRAGG--------------------------------------LEVAVVIDEALDAG 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 189 LRDGARQPFQPaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIAnILQNDAWSQPA-----LRKAPQ-IDALVtvcalpl 262
Cdd:cd12117 125 PAGNPAVPVSP-----DDLAYVMYTSGSTGRPKGVAVTHRGVVR-LVKNTNYVTLGpddrvLQTSPLaFDAST------- 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 263 yhiLALNACMLMGARwgamnlLIPNPRDVDGFVKELSRYRVNffPGVNTLF--NALLNHpgFAALD---FSGLRITLGGG 337
Cdd:cd12117 192 ---FEIWGALLNGAR------LVLAPKGTLLDPDALGALIAE--EGVTVLWltAALFNQ--LADEDpecFAGLRELLTGG 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 338 mavqEVVSTRWMKTT--GCP---ILEGYGLSETSPSVTLSPPTLGAFTGT---IGLPVPSTDIALLGENDEPVAQGQPGE 409
Cdd:cd12117 259 ----EVVSPPHVRRVlaACPglrLVNGYGPTENTTFTTSHVVTELDEVAGsipIGRPIANTRVYVLDEDGRPVPPGVPGE 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 410 IAIRGPQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPG 483
Cdd:cd12117 335 LYVGGDGLALGYLNRPALTAERFVADPFgpgerlYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPG 414
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 484 VIECAcVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd12117 415 VREAV-VVVREDAGGDKRLVAYVVAEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
50-555 |
1.49e-48 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 176.70 E-value: 1.49e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDsgaeaivvLEN 129
Cdd:cd05906 41 SYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVPPTYDEPNARLRK--------LRH 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 FATTLQQVIARTDVRhvVVASLGDLLG--PVRGMAVNFVArrvkklvppyrlpgatpfrtALRDGARQPFQPAALNhDDV 207
Cdd:cd05906 113 IWQLLGSPVVLTDAE--LVAEFAGLETlsGLPGIRVLSIE--------------------ELLDTAADHDLPQSRP-DDL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 208 AFLQYTGGTTGVSKGATLTHRNVIANILQNdAWSQPALRKApqidalVTVCALPLYHILALNACMLMGARWGAMNLLIPN 287
Cdd:cd05906 170 ALLMLTSGSTGFPKAVPLTHRNILARSAGK-IQHNGLTPQD------VFLNWVPLDHVGGLVELHLRAVYLGCQQVHVPT 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 288 P---RDVDGFVKELSRYRV------NFFpgvNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVS---TRWMKTTGCP 355
Cdd:cd05906 243 EeilADPLRWLDLIDRYRVtitwapNFA---FALLNDLLEEIEDGTWDLSSLRYLVNAGEAVVAKTIrrlLRLLEPYGLP 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 356 ---ILEGYGLSETSPSVT--LSPPTLGAFTGT----IGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPD 426
Cdd:cd05906 320 pdaIRPAFGMTETCSGVIysRSFPTYDHSQALefvsLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPE 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 427 ETAKVMTPDGFFKSGDVGVMDeHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIE---CAC-VGVPDSNSGEAVK 502
Cdd:cd05906 400 ANAEAFTEDGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPsftAAFaVRDPGAETEELAI 478
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 503 LFVVRRDPALTTEALMAYCK----KEFTGykKPKYI--EFRDSLPKSNIGKILRRELRD 555
Cdd:cd05906 479 FFVPEYDLQDALSETLRAIRsvvsREVGV--SPAYLipLPKEEIPKTSLGKIQRSKLKA 535
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
45-548 |
2.63e-47 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 177.81 E-value: 2.63e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 45 MGRTMTYAELDTYSRHLGAWLQlRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNplYTPRE--LEHQLRDSGAE 122
Cdd:PRK08633 638 TGGELSYGKALTGALALARLLK-RELKDEENVGILLPPSVAGALANLALLLAGKVPVNLN--YTASEaaLKSAIEQAQIK 714
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 123 AIV----VLENFATTLQQVIARTDVRHVVVASLGDLLGPVRGMAVNFVARrvkklVPPYRLpgatpfrtaLRDGARQPFQ 198
Cdd:PRK08633 715 TVItsrkFLEKLKNKGFDLELPENVKVIYLEDLKAKISKVDKLTALLAAR-----LLPARL---------LKRLYGPTFK 780
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 PaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRkapqiDALVTvcALPLYHILALNACMLMGARW 278
Cdd:PRK08633 781 P-----DDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRND-----DVILS--SLPFFHSFGLTVTLWLPLLE 848
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILE 358
Cdd:PRK08633 849 GIKVVYHPDPTDALGIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMFASLRLVVAGAEKLKPEVADAFEEKFGIRILE 928
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTLS-PPTL--------GAFTGTIGLPVPSTDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDET 428
Cdd:PRK08633 929 GYGATETSPVASVNlPDVLaadfkrqtGSKEGSVGMPLPGVAVRIVDpETFEELPPGEDGLILIGGPQVMKGYLGDPEKT 1008
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 429 AKVMT---PDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAA--HPGVIECACVGVPDSNSGEavKL 503
Cdd:PRK08633 1009 AEVIKdidGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKalGGEEVVFAVTAVPDEKKGE--KL 1086
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 979216869 504 FVVRRDPALTTEALMAYCKK-EFTGYKKPKYIEFRDSLPKSNIGKI 548
Cdd:PRK08633 1087 VVLHTCGAEDVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKL 1132
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
212-542 |
2.97e-47 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 167.86 E-value: 2.97e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 212 YTGGTTGVSKGATLTHRNVIANILqNDAWSQpalrkapQID-ALVTVCALPLYHILALNACM---LMGARwgamNLLIP- 286
Cdd:cd17636 7 YTAAFSGRPNGALLSHQALLAQAL-VLAVLQ-------AIDeGTVFLNSGPLFHIGTLMFTLatfHAGGT----NVFVRr 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 287 -NPRDVDGFVkELSRYRVNFFPGVNTLFNALLNHPGfaALDFSGLRITLG--GGMAVQEVVSTRWMKTTGcpileGYGLS 363
Cdd:cd17636 75 vDAEEVLELI-EAERCTHAFLLPPTIDQIVELNADG--LYDLSSLRSSPAapEWNDMATVDTSPWGRKPG-----GYGQT 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 364 ETSPSVTLSPPTLGAfTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDV 443
Cdd:cd17636 147 EVMGLATFAALGGGA-IGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRTR-GGWHHTNDL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 444 GVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCK 522
Cdd:cd17636 225 GRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGAsVTEAELIEHCR 304
|
330 340
....*....|....*....|
gi 979216869 523 KEFTGYKKPKYIEFRDSLPK 542
Cdd:cd17636 305 ARIASYKKPKSVEFADALPR 324
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
41-554 |
6.64e-47 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 172.10 E-value: 6.64e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 41 AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGftVVNVNPLYTPRELEhqlrdsg 120
Cdd:PRK10946 41 AVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLG--VAPVNALFSHQRSE------- 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 121 aeaivvLENFATTLQQVIARTDVRHVVVAS---LGDLLGPVRGMAVNFVARRvkklvppyrlPGATPFRTALRDGArQPF 197
Cdd:PRK10946 112 ------LNAYASQIEPALLIADRQHALFSDddfLNTLVAEHSSLRVVLLLND----------DGEHSLDDAINHPA-EDF 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 198 QPAALNHDDVAFLQYTGGTTGVSKGATLTHrnvianilqNDAWSqpALRKAPQIDALVT----VCALPLYHILALNACML 273
Cdd:PRK10946 175 TATPSPADEVAFFQLSGGSTGTPKLIPRTH---------NDYYY--SVRRSVEICGFTPqtryLCALPAAHNYPMSSPGA 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 274 MGARW-GAMNLLIPNPRDVDGFvKELSRYRVN---FFPGVNTLFNALLNHPGFAAlDFSGLRITLGGGMAVQEVVSTRWM 349
Cdd:PRK10946 244 LGVFLaGGTVVLAPDPSATLCF-PLIEKHQVNvtaLVPPAVSLWLQAIAEGGSRA-QLASLKLLQVGGARLSETLARRIP 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPILEGYGLSETSPSVT-LSPPTLGAFTgTIGLPV-PSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDE 427
Cdd:PRK10946 322 AELGCQLQQVFGMAEGLVNYTrLDDSDERIFT-TQGRPMsPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQH 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 428 TAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVR 507
Cdd:PRK10946 401 NASAFDANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVV 480
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 979216869 508 RDPaLTTEALMAYCKKE-FTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK10946 481 KEP-LKAVQLRRFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLR 527
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
206-555 |
1.41e-46 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 165.97 E-value: 1.41e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANIlqndAWSQPALRKAPQIDALvtvCALPLYHI--LA-LNACMLMGArwgamN 282
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASA----AGLHSRLGFGGGDSWL---LSLPLYHVggLAiLVRSLLAGA-----E 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLIPNPRDvdGFVKELSRYRVNFFPGVNT-LFNALLNHPGFAALDfsGLR-ITLGGGMAVQEVvsTRWMKTTGCPILEGY 360
Cdd:cd17630 69 LVLLERNQ--ALAEDLAPPGVTHVSLVPTqLQRLLDSGQGPAALK--SLRaVLLGGAPIPPEL--LERAADRGIPLYTTY 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 361 GLSETSPSVTLSPPtLGAFTGTIGLPVPSTDIALLgendepvaqgQPGEIAIRGPQVMAGYWN--RPDETAKvmtpDGFF 438
Cdd:cd17630 143 GMTETASQVATKRP-DGFGRGGVGVLLPGRELRIV----------EDGEIWVGGASLAMGYLRgqLVPEFNE----DGWF 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 439 KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPAlTTEALM 518
Cdd:cd17630 208 TTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPA-DPAELR 286
|
330 340 350
....*....|....*....|....*....|....*..
gi 979216869 519 AYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd17630 287 AWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRA 323
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
50-554 |
8.97e-46 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 166.91 E-value: 8.97e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYT---PRELEHQLRDSGAEAIVV 126
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVIC---PLFSafgPEAIRDRLENSEAKVLIT 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 LENFATtlqqviaRTDVrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnhDD 206
Cdd:cd05969 79 TEELYE-------RTDP-------------------------------------------------------------ED 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 VAFLQYTGGTTGVSKGATLTHRNVIANiLQNDAWSqpaLRKAPQIDALVTvcALPLYhilALNACMLMGARW--GAMNLL 284
Cdd:cd05969 91 PTLLHYTSGTTGTPKGVLHVHDAMIFY-YFTGKYV---LDLHPDDIYWCT--ADPGW---VTGTVYGIWAPWlnGVTNVV 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 285 IPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGF--AALDFSGLRITLGGGMAVQ-EVVstRW-MKTTGCPILEGY 360
Cdd:cd05969 162 YEGRFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDElaRKYDLSSLRFIHSVGEPLNpEAI--RWgMEVFGVPIHDTW 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 361 GLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG--PQVMAGYWNRPdETAKVMTPDGFF 438
Cdd:cd05969 240 WQTETGSIMIANYPCMPIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPgwPSMFRGIWNDE-ERYKNSFIDGWY 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 439 KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEAL- 517
Cdd:cd05969 319 LTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELk 398
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 979216869 518 ---MAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05969 399 eeiINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLK 438
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
12-555 |
1.64e-45 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 168.40 E-value: 1.64e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 12 GVPATVDPDRY-PSLAHLLDES------FATFAARnayaCMGR--------TMTYAELDTYSRHLGAWLQLRGLRKGARV 76
Cdd:PRK13382 21 GLIAPMRPDRYlRIVAAMRREGmgptsgFAIAAQR----CPDRpglidelgTLTWRELDERSDALAAALQALPIGEPRVV 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 77 AVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQviartdvrhvvvaSLGDLLG 156
Cdd:PRK13382 97 GIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIYDEEFSATVDR-------------ALADCPQ 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 157 PVRgmAVNFVARRVKKLVppyrlpgatpfrTALRDGARQPFQPAALNHDDVAFLqyTGGTTGVSKGATLTHR---NVIAN 233
Cdd:PRK13382 164 ATR--IVAWTDEDHDLTV------------EVLIAAHAGQRPEPTGRKGRVILL--TSGTTGTPKGARRSGPggiGTLKA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 234 ILQNDAWsqpalrKAPQidalVTVCALPLYHI---------LALNACMLMGARWgamnllipnprDVDGFVKELSRYRVN 304
Cdd:PRK13382 228 ILDRTPW------RAEE----PTVIVAPMFHAwgfsqlvlaASLACTIVTRRRF-----------DPEATLDLIDRHRAT 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 305 FFPGVNTLFNALLNHP--GFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGT 382
Cdd:PRK13382 287 GLAVVPVMFDRIMDLPaeVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIATATPADLRAAPDT 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 383 IGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETakvmTPDGFFKSGDVGVMDEHGFVRIVDRKKDMI 462
Cdd:PRK13382 367 AGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGYTSGSTKD----FHDGFMASGDVGYLDENGRLFVVGRDDEMI 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 463 LVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV-RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLP 541
Cdd:PRK13382 443 VSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVlKPGASATPETLKQHVRDNLANYKVPRDIVVLDELP 522
|
570
....*....|....
gi 979216869 542 KSNIGKILRRELRD 555
Cdd:PRK13382 523 RGATGKILRRELQA 536
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
38-553 |
2.39e-45 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 166.31 E-value: 2.39e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 38 ARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYtPRE-LEHQL 116
Cdd:cd12116 2 DATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDY-PADrLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 117 RDSGAEAIvvlenfattlqqvIARTDVRHvvvaslgdllgpvrgmavnfvarrvkklvpPYRLPGATPFRTALRDGARQP 196
Cdd:cd12116 81 EDAEPALV-------------LTDDALPD------------------------------RLPAGLPVLLLALAAAAAAPA 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 197 FQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNViANILQNDAwSQPALRKAPQIDALVTVC----ALPLYHILalnacm 272
Cdd:cd12116 118 APRTPVSPDDLAYVIYTSGSTGRPKGVVVSHRNL-VNFLHSMR-ERLGLGPGDRLLAVTTYAfdisLLELLLPL------ 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 273 LMGARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNhPGFAALDfsGLRItLGGGMAVQEVVSTRWM 349
Cdd:cd12116 190 LAGAR-----VVIAPRetqRDPEALARLIEAHSITVMQATPATWRMLLD-AGWQGRA--GLTA-LCGGEALPPDLAARLL 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCpILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETA 429
Cdd:cd12116 261 SRVGS-LWNLYGPTETTIWSTAARVTAAAGPIPIGRPLANTQVYVLDAALRPVPPGVPGELYIGGDGVAQGYLGRPALTA 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 430 KVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVK 502
Cdd:cd12116 340 ERFVPDPFagpgsrlYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDGGDRRLVA 419
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 503 LFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd12116 420 YVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
46-553 |
3.28e-45 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 166.30 E-value: 3.28e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAeaiv 125
Cdd:cd17646 21 GRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPADRLAYMLADAGP---- 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfattlqqviartdvrhVVVASLGDLLGPVRGMAVNFVARRVKKLVPPYRLPGATPfrtalrdgarqpfQPAALnhd 205
Cdd:cd17646 97 --------------------AVVLTTADLAARLPAGGDVALLGDEALAAPPATPPLVPP-------------RPDNL--- 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 dvAFLQYTGGTTGVSKGATLTHRNvIANILqndAWSQPA---------LRKAP-QIDALVTVCALPLYHilalnacmlmG 275
Cdd:cd17646 141 --AYVIYTSGSTGRPKGVMVTHAG-IVNRL---LWMQDEyplgpgdrvLQKTPlSFDVSVWELFWPLVA----------G 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 276 ARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDfsGLRITLGGGMAVQEVVSTRWMKTT 352
Cdd:cd17646 205 AR-----LVVARPgghRDPAYLAALIREHGVTTCHFVPSMLRVFLAEPAAGSCA--SLRRVFCSGEALPPELAARFLALP 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 353 GCPILEGYGLSETSPSVTLSPPTLGAFTGT--IGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAK 430
Cdd:cd17646 278 GAELHNLYGPTEAAIDVTHWPVRGPAETPSvpIGRPVPNTRLYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAE 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 431 VMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEcACVGVPDSNSGEAVKL- 503
Cdd:cd17646 358 RFVPDPFgpgsrmYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTH-AVVVARAAPAGAARLVg 436
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 979216869 504 FVVRRD--PALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17646 437 YVVPAAgaAGPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
33-554 |
3.47e-45 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 166.37 E-value: 3.47e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 33 FATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYT 108
Cdd:cd17651 1 FERQAARTpdapALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 109 PRELEHQLRDSGAEAIVvlenfattLQQViartdvrhvvvasLGDLLGPVRGMavnfvarrvkkLVPPYRLPGATPFRTA 188
Cdd:cd17651 81 AERLAFMLADAGPVLVL--------THPA-------------LAGELAVELVA-----------VTLLDQPGAAAGADAE 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 189 LRdgarqpfqpAALNHDDVAFLQYTGGTTGVSKGATLTHRNViANIL--QNDAWSQPALRKAPQIDAL-VTVCALPLYHI 265
Cdd:cd17651 129 PD---------PALDADDLAYVIYTSGSTGRPKGVVMPHRSL-ANLVawQARASSLGPGARTLQFAGLgFDVSVQEIFST 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 266 LALNACmlmgarwgamnLLIPNPR---DVDGFVKELSRYRVN--FFPGVntLFNALLNHPGFAALDFSGLRITLGGGMAV 340
Cdd:cd17651 199 LCAGAT-----------LVLPPEEvrtDPPALAAWLDEQRISrvFLPTV--ALRALAEHGRPLGVRLAALRYLLTGGEQL 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 341 QEVVSTRWMKTT--GCPILEGYGLSE----TSPSVTLSPPTLGAfTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG 414
Cdd:cd17651 266 VLTEDLREFCAGlpGLRLHNHYGPTEthvvTALSLPGDPAAWPA-PPPIGRPIDNTRVYVLDAALRPVPPGVPGELYIGG 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 415 PQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECA 488
Cdd:cd17651 345 AGLARGYLNRPELTAERFVPDPFvpgarmYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAV 424
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 489 CVGVPDSNSGEAVKLFVV-RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd17651 425 VLAREDRPGEKRLVAYVVgDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
18-561 |
3.58e-45 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 171.58 E-value: 3.58e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 18 DPDRYPSLAHLLDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAA 93
Cdd:COG1020 467 ATAAPYPADATLHELFEAQAARTpdavAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAV 546
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 94 LRAGFTVVNVNPLYtPRE-LEHQLRDSGAEAIVVLENFATTLqqviARTDVRHVVVASLgdllgpvrgmavnfvarrvkk 172
Cdd:COG1020 547 LKAGAAYVPLDPAY-PAErLAYMLEDAGARLVLTQSALAARL----PELGVPVLALDAL--------------------- 600
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 173 lvPPYRLPGATPfrtalrdgarqpfqPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIL---------QNDAWSQp 243
Cdd:COG1020 601 --ALAAEPATNP--------------PVPVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLAwmqrryglgPGDRVLQ- 663
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 244 alrKAPQI-DALVTVCALPLyhilalnacmLMGARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNH 319
Cdd:COG1020 664 ---FASLSfDASVWEIFGAL----------LSGAT-----LVLAPPearRDPAALAELLARHRVTVLNLTPSLLRALLDA 725
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 pgfAALDFSGLRITLGGGMAVQEVVSTRWMKTT-GCPILEGYGLSETSPSVTLSPPTLGAFTG---TIGLPVPSTDIALL 395
Cdd:COG1020 726 ---APEALPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETTVDSTYYEVTPPDADGgsvPIGRPIANTRVYVL 802
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 396 GENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFN 468
Cdd:COG1020 803 DAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFgfpgarlYRTGDLARWLPDGNLEFLGRADDQVKIRGFR 882
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 469 VYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKE-FTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:COG1020 883 IELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALlLPPYMVPAAVVLLLPLPLTGNGK 962
|
570
....*....|....
gi 979216869 548 ILRRELRDEHGQTA 561
Cdd:COG1020 963 LDRLALPAPAAAAA 976
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
46-554 |
6.43e-45 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 165.95 E-value: 6.43e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK13390 22 GEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVLV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VlenfATTLQQVIARTDvrhvvvaslGDLlgPVRgmaVNFVARrvkklvppyrLPGATPFRTALRD-GARQPFQPAAlnh 204
Cdd:PRK13390 102 A----SAALDGLAAKVG---------ADL--PLR---LSFGGE----------IDGFGSFEAALAGaGPRLTEQPCG--- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 ddvAFLQYTGGTTGVSKG--ATLTHRNVianilqnDAWSQPALRKAPQI----DALVTVCALPLYHILALNACMLMGARW 278
Cdd:PRK13390 151 ---AVMLYSSGTTGFPKGiqPDLPGRDV-------DAPGDPIVAIARAFydisESDIYYSSAPIYHAAPLRWCSMVHALG 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAmnLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALL--NHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPI 356
Cdd:PRK13390 221 GT--VVLAKRFDAQATLGHVERYRITVTQMVPTMFVRLLklDADVRTRYDVSSLRAVIHAAAPCPVDVKHAMIDWLGPIV 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 357 LEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPStDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDG 436
Cdd:PRK13390 299 YEYYSSTEAHGMTFIDSPDWLAHPGSVGRSVLG-DLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPAH 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 FFKS--GDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV-----VRRD 509
Cdd:PRK13390 378 PFWTtvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIqlvegIRGS 457
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 979216869 510 PALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK13390 458 DELARE-LIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
47-549 |
2.60e-43 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 163.13 E-value: 2.60e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAEL-DTYSRHLGAWLQLrGLRKGARVAVMMPNVLQYPVAVAAALRAG--FTVVNVNplYTPRELEHQLRDSGAEA 123
Cdd:cd17634 83 RTISYRELhREVCRFAGTLLDL-GVKKGDRVAIYMPMIPEAAVAMLACARIGavHSVIFGG--FAPEAVAGRIIDSSSRL 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 124 IVVLENFATTLQQViartDVRHVVVASLGDLLGPVRGMAVnfvarrVKKLVPPYR-LPGATPFRTALRDGARQPFQPAAL 202
Cdd:cd17634 160 LITADGGVRAGRSV----PLKKNVDDALNPNVTSVEHVIV------LKRTGSDIDwQEGRDLWWRDLIAKASPEHQPEAM 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTH-----------RNVIaNILQNDAWSQPAlrkapqiD-ALVTVCALPLYHILALNA 270
Cdd:cd17634 230 NAEDPLFILYTSGTTGKPKGVLHTTggylvyaattmKYVF-DYGPGDIYWCTA-------DvGWVTGHSYLLYGPLACGA 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 271 CMLM--GArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALL--NHPGFAALDFSGLRITLGGGMAVQEVV-- 344
Cdd:cd17634 302 TTLLyeGV---------PNWPTPARMWQVVDKHGVNILYTAPTAIRALMaaGDDAIEGTDRSSLRILGSVGEPINPEAye 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 345 -STRWMKTTGCPILEGYGLSETSPS-VTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGP---QVMA 419
Cdd:cd17634 373 wYWKKIGKEKCPVVDTWWQTETGGFmITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPwpgQTRT 452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 GYWNRPD-ETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSG 498
Cdd:cd17634 453 LFGDHERfEQTYFSTFKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKG 532
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 499 EAVKLFVVRR-----DPALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKIL 549
Cdd:cd17634 533 QAPYAYVVLNhgvepSPELYAE-LRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
25-554 |
4.69e-43 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 162.66 E-value: 4.69e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAH-LLDESFATFAARNAYACMG-----RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGF 98
Cdd:cd05968 62 IVEqLLDKWLADTRTRPALRWEGedgtsRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGG 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 99 TVVNVNPLYTPRELEHQLRDSGAEAIVVLENFA----------TTLQQVIARTDVRHVVVaslgdllgpVRGMAVNFVAR 168
Cdd:cd05968 142 IVVPIFSGFGKEAAATRLQDAEAKALITADGFTrrgrevnlkeEADKACAQCPTVEKVVV---------VRHLGNDFTPA 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 169 RVKKLVPPYRLpgATPFRTALRDGARQPFqpaalnhddvaFLQYTGGTTGVSKGATLTHRNVIANILQnDAWSQPALRKA 248
Cdd:cd05968 213 KGRDLSYDEEK--ETAGDGAERTESEDPL-----------MIIYTSGTTGKPKGTVHVHAGFPLKAAQ-DMYFQFDLKPG 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 249 pqiDALVTVCAL-----P--LYHILALNACMLM--GArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNH 319
Cdd:cd05968 279 ---DLLTWFTDLgwmmgPwlIFGGLILGATMVLydGA---------PDHPKADRLWRMVEDHEITHLGLSPTLIRALKPR 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 --PGFAALDFSGLRItLGGGMAVQEVVSTRWM-KTTG---CPILEGYGLSETSP----SVTLSPPTLGAFTGtiglPVPS 389
Cdd:cd05968 347 gdAPVNAHDLSSLRV-LGSTGEPWNPEPWNWLfETVGkgrNPIINYSGGTEISGgilgNVLIKPIKPSSFNG----PVPG 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 390 TDIALLGENDEPVaQGQPGEIAIRGPQV-MA-GYWNRPD---ET--AKVmtpDGFFKSGDVGVMDEHGFVRIVDRKKDMI 462
Cdd:cd05968 422 MKADVLDESGKPA-RPEVGELVLLAPWPgMTrGFWRDEDrylETywSRF---DNVWVHGDFAYYDEEGYFYILGRSDDTI 497
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 463 LVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEA----LMAYCKKEFTGYKKPKYIEFRD 538
Cdd:cd05968 498 NVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEAlaeeLMERVADELGKPLSPERILFVK 577
|
570
....*....|....*.
gi 979216869 539 SLPKSNIGKILRRELR 554
Cdd:cd05968 578 DLPKTRNAKVMRRVIR 593
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
15-561 |
3.68e-42 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 158.90 E-value: 3.68e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 15 ATVDPDRYPSLAHLLDESFATFAARNAYACMGR--TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAA 92
Cdd:PRK05852 8 APMASDFGPRIADLVEVAATRLPEAPALVVTADriAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 93 ALRAGFTVVNVNPLYTPRELEHQLRDSGAEaiVVLENFATTLQQVIARTDVRHVVVAslgdlLGPVRGMAVNFVARRVKK 172
Cdd:PRK05852 88 ASRADLVVVPLDPALPIAEQRVRSQAAGAR--VVLIDADGPHDRAEPTTRWWPLTVN-----VGGDSGPSGGTLSVHLDA 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 173 LVPPyrlpgaTPFRTAlrdgarqpfqPAALNHDDvAFLQYTGGTTGVSKGATLTHRNVIANILQNDAwsqpALRKAPQiD 252
Cdd:PRK05852 161 ATEP------TPATST----------PEGLRPDD-AMIMFTGGTTGLPKMVPWTHANIASSVRAIIT----GYRLSPR-D 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 253 AlvTVCALPLYHILALNACMLMGARWGAMNLLIPNPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHP-------GFAA 324
Cdd:PRK05852 219 A--TVAVMPLYHGHGLIAALLATLASGGAVLLPARGRfSAHTFWDDIKAVGATWYTAVPTIHQILLERAatepsgrKPAA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 325 LDF---SGLRITLGGGMAVQEvvstrwmkTTGCPILEGYGLSETSPSVTlSPPTLGAFTG-----TIGLPVPST--DIAL 394
Cdd:PRK05852 297 LRFirsCSAPLTAETAQALQT--------EFAAPVVCAFGMTEATHQVT-TTQIEGIGQTenpvvSTGLVGRSTgaQIRI 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 395 LGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEI 474
Cdd:PRK05852 368 VGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFT-DGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERV 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 475 ESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK05852 447 EGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESApPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
|
....*...
gi 979216869 554 RDEHGQTA 561
Cdd:PRK05852 527 AEQFGHSV 534
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
47-554 |
1.28e-41 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 157.75 E-value: 1.28e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGfTVVNvnPLYT---PRELEHQLRDSGAEA 123
Cdd:PRK04319 72 EKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNG-AIVG--PLFEafmEEAVRDRLEDSEAKV 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 124 IVvlenfaTT---LQQVIAR--TDVRHV-VVASLGDLLGPVRGMAvnfvarrvkklvppyrlpgatpfrtALRDGARQPF 197
Cdd:PRK04319 149 LI------TTpalLERKPADdlPSLKHVlLVGEDVEEGPGTLDFN-------------------------ALMEQASDEF 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 198 QPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANI--------LQND---------AWsqpalrkapqidalVTVCAl 260
Cdd:PRK04319 198 DIEWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYqtgkyvldLHEDdvywctadpGW--------------VTGTS- 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 261 plYHILA--LNacmlmgarwGAMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPG--FAALDFSGLRITLGG 336
Cdd:PRK04319 263 --YGIFApwLN---------GATNVIDGGRFSPERWYRILEDYKVTVWYTAPTAIRMLMGAGDdlVKKYDLSSLRHILSV 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQ-EVVstRW-MKTTGCPILEGYGLSETS-------PSVTLSPptlgaftGTIGLPVPSTDIALLGENDEPVAQGQP 407
Cdd:PRK04319 332 GEPLNpEVV--RWgMKVFGLPIHDNWWMTETGgimianyPAMDIKP-------GSMGKPLPGIEAAIVDDQGNELPPNRM 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 408 GEIAIRG--PQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVI 485
Cdd:PRK04319 403 GNLAIKKgwPSMMRGIWNNPEKYESYFA-GDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVA 481
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 486 ECACVGVPDSNSGEAVKLFVVRRDPALTTEAL----MAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK04319 482 EAGVIGKPDPVRGEIIKAFVALRPGYEPSEELkeeiRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLK 554
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
39-553 |
2.71e-41 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 154.77 E-value: 2.71e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 39 RNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD 118
Cdd:cd17643 3 AVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFILAD 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 119 SGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfQ 198
Cdd:cd17643 83 SGPSLLLT-----------------------------------------------------------------------D 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 PaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndAWSQPALRKAPQiDALVtvcalpLYHILALN-------AC 271
Cdd:cd17643 92 P-----DDLAYVIYTSGSTGRPKGVVVSHANVLALF----AATQRWFGFNED-DVWT------LFHSYAFDfsvweiwGA 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 272 MLMGARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRW 348
Cdd:cd17643 156 LLHGGR-----LVVVPYevaRSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPLALRYVIFGGEALEAAMLRPW 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 349 MKTTG--CPIL-EGYGLSETSPSVT---LSPPTLGAFTG-TIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGY 421
Cdd:cd17643 231 AGRFGldRPQLvNMYGITETTVHVTfrpLDAADLPAAAAsPIGRPLPGLRVYVLDADGRPVPPGVVGELYVSGAGVARGY 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 422 WNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEcACVGVPD 494
Cdd:cd17643 311 LGRPELTAERFVANPFggpgsrmYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRD-AAVIVRE 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 979216869 495 SNSGEA--VKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17643 390 DEPGDTrlVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
212-550 |
7.61e-41 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 150.25 E-value: 7.61e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 212 YTGGTTGVSKGATLTHRNVIAnilqndawSQPALRKAPQIDALVTVCAL-PLYHILALNACMlmGARWGAMNLLIPNPRD 290
Cdd:cd17633 7 FTSGTTGLPKAYYRSERSWIE--------SFVCNEDLFNISGEDAILAPgPLSHSLFLYGAI--SALYLGGTFIGQRKFN 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 291 VDGFVKELSRYRVNFFPGVNTLFNALLNHpgfaALDFSGLRITLGGGMAVQE----VVSTRWMKTTgcpILEGYGLSETS 366
Cdd:cd17633 77 PKSWIRKINQYNATVIYLVPTMLQALART----LEPESKIKSIFSSGQKLFEstkkKLKNIFPKAN---LIEFYGTSELS 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 367 pSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDepvaqGQPGEIAIRGPQVMAGYWnrpdeTAKVMTPDGFFKSGDVGVM 446
Cdd:cd17633 150 -FITYNFNQESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYV-----RGGFSNPDGWMSVGDIGYV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 447 DEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVkLFVVRRDpALTTEALMAYCKKEFT 526
Cdd:cd17633 219 DEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIA-VALYSGD-KLTYKQLKRFLKQKLS 296
|
330 340
....*....|....*....|....
gi 979216869 527 GYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:cd17633 297 RYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
205-475 |
1.20e-40 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 153.28 E-value: 1.20e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPalrKAPQIdalvTVCALPLYHIlALNACMLMGARWGaMNLL 284
Cdd:cd17640 88 DDLATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPP---QPGDR----FLSILPIWHS-YERSAEYFIFACG-CSQA 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 285 IPNPRDvdgFVKELSRYRVNFFPGVNTLFNALLN--HPGFAALDFSGLRITL-------------GGGMAVQEVVstRWM 349
Cdd:cd17640 159 YTSIRT---LKDDLKRVKPHYIVSVPRLWESLYSgiQKQVSKSSPIKQFLFLfflsggifkfgisGGGALPPHVD--TFF 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPILEGYGLSETSPSVT---LSPPTLGaftgTIGLPVPSTDIALLGEN-DEPVAQGQPGEIAIRGPQVMAGYWNRP 425
Cdd:cd17640 234 EAIGIEVLNGYGLTETSPVVSarrLKCNVRG----SVGRPLPGTEIKIVDPEgNVVLPPGEKGIVWVRGPQVMKGYYKNP 309
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 426 DETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS-GFNVYPNEIE 475
Cdd:cd17640 310 EATSKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIE 360
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
203-559 |
1.22e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 153.80 E-value: 1.22e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTHRNVIANILqndawsqpALRKAPQIDALVTVCA-LPLYHILALNACMLMGARWGAM 281
Cdd:cd05908 104 LADELAFIQFSSGSTGDPKGVMLTHENLVHNMF--------AILNSTEWKTKDRILSwMPLTHDMGLIAFHLAPLIAGMN 175
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNP---RDVDGFVKELSRYRVNFFPGVN---TLFNALLNHPGFAALDFSGLRITLGGGMAVQ--------EVVSTR 347
Cdd:cd05908 176 QYLMPTRlfiRRPILWLKKASEHKATIVSSPNfgyKYFLKTLKPEKANDWDLSSIRMILNGAEPIDyelcheflDHMSKY 255
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 348 WMKTTGcpILEGYGLSETSPSVTLSPPTLGAFTGTI---------------------------GLPVPSTDIALLGENDE 400
Cdd:cd05908 256 GLKRNA--ILPVYGLAEASVGASLPKAQSPFKTITLgrrhvthgepepevdkkdsecltfvevGKPIDETDIRICDEDNK 333
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 401 PVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVrIVDRKKDMILVSGFNVYPNEIESVVAA 480
Cdd:cd05908 334 ILPDGYIGHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIRNGRLV-ITGREKDIIFVNGQNVYPHDIERIAEE 412
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 481 HPGVI--ECACVGVPDSNS-GEAVKLFVVRRDPAlttEALMAYCKK------EFTGYKKPKYIEFRdSLPKSNIGKILRR 551
Cdd:cd05908 413 LEGVElgRVVACGVNNSNTrNEEIFCFIEHRKSE---DDFYPLGKKikkhlnKRGGWQINEVLPIR-RIPKTTSGKVKRY 488
|
....*...
gi 979216869 552 ELRDEHGQ 559
Cdd:cd05908 489 ELAQRYQS 496
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
194-556 |
1.23e-40 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 154.96 E-value: 1.23e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 194 RQPFQPAALNH----DDVAFLQYTGGTTGVSKGATLTHRNVIANILqndawSQPALRKAPQIDalVTVCALPLYHILALN 269
Cdd:PLN02860 157 QRALGTTELDYawapDDAVLICFTSGTTGRPKGVTISHSALIVQSL-----AKIAIVGYGEDD--VYLHTAPLCHIGGLS 229
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 270 AC--MLMGarwGAMNLLIPNpRDVDGFVKELSRYRVNFFPGVNTLFNALL--NHPGFAALDFSGLRITLGGGMAVQEVVS 345
Cdd:PLN02860 230 SAlaMLMV---GACHVLLPK-FDAKAALQAIKQHNVTSMITVPAMMADLIslTRKSMTWKVFPSVRKILNGGGSLSSRLL 305
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 346 TRWMKTTGCP-ILEGYGLSETSPSVT---LSPPTLGAFTGT-------------------IGLPVPSTDIALLGENDEPV 402
Cdd:PLN02860 306 PDAKKLFPNAkLFSAYGMTEACSSLTfmtLHDPTLESPKQTlqtvnqtksssvhqpqgvcVGKPAPHVELKIGLDESSRV 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 aqgqpGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHP 482
Cdd:PLN02860 386 -----GRILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHP 460
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 483 GVIECACVGVPDSNSGEAVKLFVVRRDP---------------ALTTEALMAYC-KKEFTGYKKPK-YIEFRDSLPKSNI 545
Cdd:PLN02860 461 GVASVVVVGVPDSRLTEMVVACVRLRDGwiwsdnekenakknlTLSSETLRHHCrEKNLSRFKIPKlFVQWRKPFPLTTT 540
|
410
....*....|.
gi 979216869 546 GKILRRELRDE 556
Cdd:PLN02860 541 GKIRRDEVRRE 551
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
203-490 |
3.28e-40 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 152.75 E-value: 3.28e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPqidalvTVCA-LPLYHILAL---NACMLMGARW 278
Cdd:cd17639 86 KPDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRVPELLGPDD------RYLAyLPLAHIFELaaeNVCLYRGGTI 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 G---AMNLLIPNPRDVDGfvkELSRYRVNFFPGV----------------------NTLF--------NALLNHPGFAAL 325
Cdd:cd17639 160 GygsPRTLTDKSKRGCKG---DLTEFKPTLMVGVpaiwdtirkgvlaklnpmgglkRTLFwtayqsklKALKEGPGTPLL 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 326 D---FSG--------LRITLGGGMAVQEvvST-RWMKTTGCPILEGYGLSETSPSVTLSPPtlGAF-TGTIGLPVPSTDI 392
Cdd:cd17639 237 DelvFKKvraalggrLRYMLSGGAPLSA--DTqEFLNIVLCPVIQGYGLTETCAGGTVQDP--GDLeTGRVGPPLPCCEI 312
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 393 ALLG------ENDEPVAQGqpgEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMI-LVS 465
Cdd:cd17639 313 KLVDweeggySTDKPPPRG---EILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKKDLVkLQN 389
|
330 340
....*....|....*....|....*
gi 979216869 466 GFNVYPNEIESVVAAHPgVIECACV 490
Cdd:cd17639 390 GEYIALEKLESIYRSNP-LVNNICV 413
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
49-554 |
7.10e-40 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 150.36 E-value: 7.10e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYT---PRELEHQLRDSGAEAIV 125
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQ---PLFTafgPKAIEHRLRTSGARLVV 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VlenfattlqqviaRTDVRHvvvaslgdllgpvrgmavnfvarrvkKLvppyrlpgatpfrtalrdgarqpfqpaalnHD 205
Cdd:cd05973 78 T-------------DAANRH--------------------------KL------------------------------DS 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNvianILQNDAWSQPALRKAPQiDALVTVC----ALPLYHilALNACMLMGARwgam 281
Cdd:cd05973 89 DPFVMMFTSGTTGLPKGVPVPLRA----LAAFGAYLRDAVDLRPE-DSFWNAAdpgwAYGLYY--AITGPLALGHP---- 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSG-LRITLGGGMAVQ-EVVstRWMKTT-GCPILE 358
Cdd:cd05973 158 TILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLLMAAGAEVPARPKGrLRRVSSAGEPLTpEVI--RWFDAAlGVPIHD 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVT----LSPPTlgaFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAI---RGPQV-MAGYWNRPDETak 430
Cdd:cd05973 236 HYGQTELGMVLAnhhaLEHPV---HAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIdiaNSPLMwFRGYQLPDTPA-- 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 431 vmtPDG-FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRD 509
Cdd:cd05973 311 ---IDGgYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRG 387
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 979216869 510 -----PALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05973 388 ghegtPALADE-LQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLR 436
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
39-553 |
1.15e-39 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 149.71 E-value: 1.15e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 39 RNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRD 118
Cdd:cd17652 3 APAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLAD 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 119 SGAEAIVvlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgATPfrtalrdgarqpfq 198
Cdd:cd17652 83 ARPALLL--------------------------------------------------------TTP-------------- 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 199 paalnhDDVAFLQYTGGTTGVSKGATLTHRNvIANILQndawSQPAlRKAPQIDALVTVCALPLYHILALNACMLMGArw 278
Cdd:cd17652 93 ------DNLAYVIYTSGSTGRPKGVVVTHRG-LANLAA----AQIA-AFDVGPGSRVLQFASPSFDASVWELLMALLA-- 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAMNLLIPNPRDVDG--FVKELSRYRVNFFpgvnTLFNALLNhpGFAALDFSGLRITLGGGMAVQEVVSTRWmkTTGCPI 356
Cdd:cd17652 159 GATLVLAPAEELLPGepLADLLREHRITHV----TLPPAALA--ALPPDDLPDLRTLVVAGEACPAELVDRW--APGRRM 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 357 LEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDG 436
Cdd:cd17652 231 INAYGPTETTVCATMAGPLPGGGVPPIGRPVPGTRVYVLDARLRPVPPGVPGELYIAGAGLARGYLNRPGLTAERFVADP 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 F-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEcACVGVPDSNSGEA--VKLFVVR 507
Cdd:cd17652 311 FgapgsrmYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAE-AVVVVRDDRPGDKrlVAYVVPA 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 979216869 508 RDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17652 390 PGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
46-555 |
2.54e-39 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 149.00 E-value: 2.54e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd17653 20 GGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQAILRTSGATLLL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpFQPAAlnhD 205
Cdd:cd17653 100 -----------------------------------------------------------------------TTDSP---D 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANILQNDA--WSQPALRKA----PQIDAlvtvCALPLYHILALNACMLmgarwg 279
Cdd:cd17653 106 DLAYIIFTSGSTGIPKGVMVPHRGVLNYVSQPPArlDVGPGSRVAqvlsIAFDA----CIGEIFSTLCNGGTLV------ 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 280 amnllipnPRDVDGFVKELSRyRVNFFPGVNTLFNALlnhpgfAALDFSGLRITLGGGMAVQEVVSTRWMKttGCPILEG 359
Cdd:cd17653 176 --------LADPSDPFAHVAR-TVDALMSTPSILSTL------SPQDFPNLKTIFLGGEAVPPSLLDRWSP--GRRLYNA 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 360 YGLSETSPSVTLSPPTLGAFTgTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-- 437
Cdd:cd17653 239 YGPTECTISSTMTELLPGQPV-TIGKPIPNSTCYILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPFwp 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 438 ----FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVpdsnSGEAVKLFVVrrdPA-L 512
Cdd:cd17653 318 gsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQAAAIV----VNGRLVAFVT---PEtV 390
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 979216869 513 TTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd17653 391 DVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
49-479 |
6.18e-39 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 149.67 E-value: 6.18e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLR--KGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYtpreleHQLrdsGAEAIV- 125
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGKpaPASFVGIYSINRPEWIISELACYAYSLVTV---PLY------DTL---GPEAIEy 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENfaTTLQQVIARTDVRhvvVASLGDLLgpvrgmavNFVARRVKKLVPPyrlpgaTPfrtalrdgarqpfqpaalnhD 205
Cdd:cd05927 74 ILNH--AEISIVFCDAGVK---VYSLEEFE--------KLGKKNKVPPPPP------KP--------------------E 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAwsqpALRKAPQIDA-LVTVCALPLYHI---LALNACMLMGARWGAM 281
Cdd:cd05927 115 DLATICYTSGTTGNPKGVMLTHGNIVSNVAGVFK----ILEILNKINPtDVYISYLPLAHIferVVEALFLYHGAKIGFY 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNPRDvDgfVKELSRYrvnFFPGV----------------------NTLFNALLN-------------HPGFAALD 326
Cdd:cd05927 191 SGDIRLLLD-D--IKALKPT---VFPGVprvlnriydkifnkvqakgplkRKLFNFALNyklaelrsgvvraSPFWDKLV 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 FSGLRITLGGgmavqevvSTRWMKTT----------------GCPILEGYGLSETSPSVTLSPPtlGAFT-GTIGLPVPS 389
Cdd:cd05927 265 FNKIKQALGG--------NVRLMLTGsaplspevleflrvalGCPVLEGYGQTECTAGATLTLP--GDTSvGHVGGPLPC 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 390 TDIAL----------LGENDEpvaqgqpGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKK 459
Cdd:cd05927 335 AEVKLvdvpemnydaKDPNPR-------GEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWLPNGTLKIIDRKK 407
|
490 500
....*....|....*....|.
gi 979216869 460 DMI-LVSGFNVYPNEIESVVA 479
Cdd:cd05927 408 NIFkLSQGEYVAPEKIENIYA 428
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
28-556 |
3.64e-38 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 147.55 E-value: 3.64e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 28 LLDESFATFAARNAyacmGRT-------------MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK07008 10 LLISSLIAHAARHA----GDTeivsrrvegdihrYTYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVS 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVNVNPLYTPRELEHQLRDsgAEAIVVL--ENFATTLQQVIAR-TDVRHVVVASLGDllgpvrgmavnfvarrvk 171
Cdd:PRK07008 86 GSGAVCHTINPRLFPEQIAYIVNH--AEDRYVLfdLTFLPLVDALAPQcPNVKGWVAMTDAA------------------ 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 172 klvppyRLP-GATPFRT--ALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRnviANILQNDAWSQPALRKA 248
Cdd:PRK07008 146 ------HLPaGSTPLLCyeTLVGAQDGDYDWPRFDENQASSLCYTSGTTGNPKGALYSHR---STVLHAYGAALPDAMGL 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 249 PQIDALVTVcaLPLYHILALN---ACMLMGARwgamnLLIPNPrDVDG-FVKEL-SRYRVNFFPGVNTLFNALLNHPGFA 323
Cdd:PRK07008 217 SARDAVLPV--VPMFHVNAWGlpySAPLTGAK-----LVLPGP-DLDGkSLYELiEAERVTFSAGVPTVWLGLLNHMREA 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 324 ALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTI----------GLPVPSTDIA 393
Cdd:PRK07008 289 GLRFSTLRRTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEMSPLGTLCKLKWKHSQLPLdeqrkllekqGRVIYGVDMK 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 394 LLGENDEPvaqgQP------GEIAIRGPQVMAGYWnRPDETAKVmtpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGF 467
Cdd:PRK07008 369 IVGDDGRE----LPwdgkafGDLQVRGPWVIDRYF-RGDASPLV---DGWFPTGDVATIDADGFMQITDRSKDVIKSGGE 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 468 NVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIG 546
Cdd:PRK07008 441 WISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAeVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATG 520
|
570
....*....|
gi 979216869 547 KILRRELRDE 556
Cdd:PRK07008 521 KLQKLKLREQ 530
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
47-556 |
1.09e-36 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 143.35 E-value: 1.09e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTmTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPrelehqlrdsgaEAIVV 126
Cdd:PRK06018 39 RT-TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFP------------EQIAW 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 LENFAttlQQVIARTDVRHV-VVASLGDLLGPVRgmavNFVARRVKKLVPPYRLPGATPFRTALrDGARQPFQPAALNHD 205
Cdd:PRK06018 106 IINHA---EDRVVITDLTFVpILEKIADKLPSVE----RYVVLTDAAHMPQTTLKNAVAYEEWI-AEADGDFAWKTFDEN 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRnviANILQNDAWSQPALRKAPQIDALVTVcaLPLYHI----LALNACMlMGArwgam 281
Cdd:PRK06018 178 TAAGMCYTSGTTGDPKGVLYSHR---SNVLHALMANNGDALGTSAADTMLPV--VPLFHAnswgIAFSAPS-MGT----- 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNPRdVDGF-VKEL-SRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTtGCPILEG 359
Cdd:PRK06018 247 KLVMPGAK-LDGAsVYELlDTEKVTFTAGVPTVWLMLLQYMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDM-GVEVRHA 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 360 YGLSETSPSVTLS--PPTLGAFTG--------TIGLPVPSTDIALLGENDEPVA-QGQ-PGEIAIRGPQVMAGYWNrpdE 427
Cdd:PRK06018 325 WGMTEMSPLGTLAalKPPFSKLPGdarldvlqKQGYPPFGVEMKITDDAGKELPwDGKtFGRLKVRGPAVAAAYYR---V 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 428 TAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV- 506
Cdd:PRK06018 402 DGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQl 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 979216869 507 RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK06018 482 KPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALREQ 531
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
48-553 |
4.14e-36 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 140.69 E-value: 4.14e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVL 127
Cdd:cd17656 13 KLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVLTQ 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 ENFATTLQQviartdvRHVVVASLGDLLGPVRGMAVNFVarrvkklvppyrlpgatpfrtalrdgarqpfqpaaLNHDDV 207
Cdd:cd17656 93 RHLKSKLSF-------NKSTILLEDPSISQEDTSNIDYI-----------------------------------NNSDDL 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 208 AFLQYTGGTTGVSKGATLTHRNvIANILQ------NDAWSQPALRKA-PQIDalvtVCalplYHILALNACMlmgarwGA 280
Cdd:cd17656 131 LYIIYTSGTTGKPKGVQLEHKN-MVNLLHferektNINFSDKVLQFAtCSFD----VC----YQEIFSTLLS------GG 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MNLLIPNP--RDVDGFVKELSRYRVN--FFPgvNTLFNALLNHPGFAALDFSGLR--ITLGGGMAVQEVVsTRWMKTTGC 354
Cdd:cd17656 196 TLYIIREEtkRDVEQLFDLVKRHNIEvvFLP--VAFLKFIFSEREFINRFPTCVKhiITAGEQLVITNEF-KEMLHEHNV 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 PILEGYGLSETS--PSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVM 432
Cdd:cd17656 273 HLHNHYGPSETHvvTTYTINPEAEIPELPPIGKPISNTWIYILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKF 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV 506
Cdd:cd17656 353 FPDPFdpnermYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV 432
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 979216869 507 rRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17656 433 -MEQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
14-553 |
4.61e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 141.29 E-value: 4.61e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 14 PATVDPDRYPSLAHLLDESFAtfaarnayacmgrtMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAA 93
Cdd:PRK13383 40 LLAVTAARWPGRTAIIDDDGA--------------LSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAV 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 94 LRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFAttlQQVIARTDVRHVVvaslgdllGPVRGMAVNFVARrvKKL 173
Cdd:PRK13383 106 GLLGADVVPISTEFRSDALAAALRAHHISTVVADNEFA---ERIAGADDAVAVI--------DPATAGAEESGGR--PAV 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 174 VPPYRLpgatpfrtalrdgarqpfqpaalnhddvafLQYTGGTTGVSKGATLTHRnVIANIlqnDAWSQPALRKAPQIDA 253
Cdd:PRK13383 173 AAPGRI------------------------------VLLTSGTTGKPKGVPRAPQ-LRSAV---GVWVTILDRTRLRTGS 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 LVTVcALPLYHILALNACMLMGARWGAmnLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPG--FAALDFSGLR 331
Cdd:PRK13383 219 RISV-AMPMFHGLGLGMLMLTIALGGT--VLTHRHFDAEAALAQASLHRADAFTAVPVVLARILELPPrvRARNPLPQLR 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 332 ITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIA 411
Cdd:PRK13383 296 VVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVGIGALATPADLRDAPETVGKPVAGCPVRILDRNNRPVGPRVTGRIF 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYwnrPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:PRK13383 376 VGGELAGTRY---TDGGGKAVV-DGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIG 451
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 492 VPDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK13383 452 VPDERFGHRLAAFVVLHPGSgVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
20-524 |
9.91e-36 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 141.78 E-value: 9.91e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 20 DRYPSLAHL--LDESFATFAARNA-YACMG------------RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVL 84
Cdd:PLN02736 35 SRFPDHPEIgtLHDNFVYAVETFRdYKYLGtrirvdgtvgeyKWMTYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRP 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 85 QYpVAVAAALRAgFTVVNVnPLYtprelehqlrDS-GAEAIVVLENFATtLQQVIARTDVRHVVVASLGDLlgPVRGMAV 163
Cdd:PLN02736 115 EW-LIVDHACSA-YSYVSV-PLY----------DTlGPDAVKFIVNHAE-VAAIFCVPQTLNTLLSCLSEI--PSVRLIV 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 164 nfVARRVKKLVPPyrLPGAT-----PFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqnd 238
Cdd:PLN02736 179 --VVGGADEPLPS--LPSGTgveivTYSKLLAQGRSSPQPFRPPKPEDVATICYTSGTTGTPKGVVLTHGNLIANV---- 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 239 AWSQPALRKAPqidALVTVCALPLYHILAlNACMLMGARWGA---------------MNLLIPN-----PR-------DV 291
Cdd:PLN02736 251 AGSSLSTKFYP---SDVHISYLPLAHIYE-RVNQIVMLHYGVavgfyqgdnlklmddLAALRPTifcsvPRlynriydGI 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 292 DGFVKELSRYRVNFFpgvNTLFN----ALLN----HPGFAALDFSGLRITLGGGMavqevvstRWMKTT----------- 352
Cdd:PLN02736 327 TNAVKESGGLKERLF---NAAYNakkqALENgknpSPMWDRLVFNKIKAKLGGRV--------RFMSSGasplspdvmef 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 353 -----GCPILEGYGLSETSpsVTLSPPTLGAFT-GTIGLPVPSTDIALLgenDEP----VAQGQP---GEIAIRGPQVMA 419
Cdd:PLN02736 396 lricfGGRVLEGYGMTETS--CVISGMDEGDNLsGHVGSPNPACEVKLV---DVPemnyTSEDQPyprGEICVRGPIIFK 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 GYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMI-LVSGFNVYPNEIESVVAAHPGVIECACVGvpDSNSG 498
Cdd:PLN02736 471 GYYKDEVQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENVYAKCKFVAQCFVYG--DSLNS 548
|
570 580
....*....|....*....|....*.
gi 979216869 499 EAVKLFVVrrDPalttEALMAYCKKE 524
Cdd:PLN02736 549 SLVAVVVV--DP----EVLKAWAASE 568
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
50-491 |
3.52e-35 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 138.76 E-value: 3.52e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV--L 127
Cdd:cd05932 8 TWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVgkL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 ENFAttlqqviartDVRHVVVASLGDLLGPvrgmavnfvarrvkklvPPYRLPGATPFRTALrdgARQPFQPAALNHDD- 206
Cdd:cd05932 88 DDWK----------AMAPGVPEGLISISLP-----------------PPSAANCQYQWDDLI---AQHPPLEERPTRFPe 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 -VAFLQYTGGTTGVSKGATLTHRNVianilqndAWSQPA----LRKAPQiDALVTVcaLPLYHIlALNACMLMGARWGAM 281
Cdd:cd05932 138 qLATLIYTSGTTGQPKGVMLTFGSF--------AWAAQAgiehIGTEEN-DRMLSY--LPLAHV-TERVFVEGGSLYGGV 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPnpRDVDGFVKELSRYRVNFFPGVN---TLF----------------------NALLNHPGFAALDFSGLRITLGG 336
Cdd:cd05932 206 LVAFA--ESLDTFVEDVQRARPTLFFSVPrlwTKFqqgvqdkipqqklnlllkipvvNSLVKRKVLKGLGLDQCRLAGCG 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVVsTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAfTGTIGLPVPSTDiallgendepVAQGQPGEIAIRGPQ 416
Cdd:cd05932 284 SAPVPPAL-LEWYRSLGLNILEAYGMTENFAYSHLNYPGRDK-IGTVGNAGPGVE----------VRISEDGEILVRSPA 351
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 417 VMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS-GFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:cd05932 352 LMMGYYKDPEATAEAFTADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
29-553 |
3.88e-35 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 137.45 E-value: 3.88e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 29 LDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:cd12115 1 LHDLVEAQAARTpdaiALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAeaivvlenfattlqqviartdvRHVVVASlgdllgpvrgmavnfvarrvkklvppyrlpgatp 184
Cdd:cd12115 81 PAYPPERLRFILEDAQA----------------------RLVLTDP---------------------------------- 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 frtalrdgarqpfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNViANILQndaWSQPALRKapqiDALVTVCA----- 259
Cdd:cd12115 105 --------------------DDLAYVIYTSGSTGRPKGVAIEHRNA-AAFLQ---WAAAAFSA----EELAGVLAstsic 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 260 --LPLYHILALNACmlmgarwGAMNLLIPNprdVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAAldfsGLR-ITLGG 336
Cdd:cd12115 157 fdLSVFELFGPLAT-------GGKVVLADN---VLALPDLPAAAEVTLINTVPSAAAELLRHDALPA----SVRvVNLAG 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAF-TGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGP 415
Cdd:cd12115 223 EPLPRDLVQRLYARLQVERVVNLYGPSEDTTYSTVAPVPPGASgEVSIGRPLANTQAYVLDRALQPVPLGVPGELYIGGA 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 416 QVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECAC 489
Cdd:cd12115 303 GVARGYLGRPGLTAERFLPDPFgpgarlYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVV 382
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 490 VGVPDSNSGEAVKLFVVRRDP-ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd12115 383 VAIGDAAGERRLVAYIVAEPGaAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
47-554 |
1.14e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 136.83 E-value: 1.14e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGlRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:PRK07638 25 RVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMIVT 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 LENFATTLqqviarTDVRHVVVASlgdllgpvrgmavnfvaRRVKKLVPPYRlpgATPFRTalRDGARQPFqpaalnhdd 206
Cdd:PRK07638 104 ERYKLNDL------PDEEGRVIEI-----------------DEWKRMIEKYL---PTYAPI--ENVQNAPF--------- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 vaFLQYTGGTTGVSKGATLTHRNVIANIlqndawsQPALRK---APQIDALVtvcALPLYHILALNAC---MLMGARWGA 280
Cdd:PRK07638 147 --YMGFTSGSTGKPKAFLRAQQSWLHSF-------DCNVHDfhmKREDSVLI---AGTLVHSLFLYGAistLYVGQTVHL 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MNLLIPNprdvdGFVKELSRYRVNFFPGVNTLFNALLNHPGFaaLDFSGLRITLGG--GMAVQEVVSTRWMKTTgcpILE 358
Cdd:PRK07638 215 MRKFIPN-----QVLDKLETENISVMYTVPTMLESLYKENRV--IENKMKIISSGAkwEAEAKEKIKNIFPYAK---LYE 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETS------PSVTLSPPTlgaftgTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKvM 432
Cdd:PRK07638 285 FYGASELSfvtalvDEESERRPN------SVGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYIIGGVLARE-L 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDpal 512
Cdd:PRK07638 358 NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSA--- 434
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 979216869 513 TTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK07638 435 TKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAK 476
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
206-550 |
1.22e-34 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 133.54 E-value: 1.22e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIA--NILQNDA--WSQPAlrkapqidalVTVCALPLYHILALnACMLMGARWGAM 281
Cdd:cd17635 2 DPLAVIFTSGTTGEPKAVLLANKTFFAvpDILQKEGlnWVVGD----------VTYLPLPATHIGGL-WWILTCLIHGGL 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR-ITLGGGMAVQ-EVVSTRWMKTTGcpILEG 359
Cdd:cd17635 71 CVTGGENTTYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRlIGYGGSRAIAaDVRFIEATGLTN--TAQV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 360 YGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFK 439
Cdd:cd17635 149 YGLSETGTALCLPTDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLI-DGWVN 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 440 SGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR--DPALTTEAL 517
Cdd:cd17635 228 TGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASaeLDENAIRAL 307
|
330 340 350
....*....|....*....|....*....|...
gi 979216869 518 MAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:cd17635 308 KHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
48-555 |
1.27e-34 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 136.52 E-value: 1.27e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYtPRE-LEHQLRDSGAeaivv 126
Cdd:cd05918 24 SLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLDPSH-PLQrLQEILQDTGA----- 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlqqviartdvrHVVVASlgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalNHDD 206
Cdd:cd05918 98 ------------------KVVLTS----------------------------------------------------SPSD 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 VAFLQYTGGTTGVSKGATLTHRNVIANILQndawSQPALRKAPQIDAL------VTVCALPLYHILALNACmlmgarwga 280
Cdd:cd05918 108 AAYVIFTSGSTGKPKGVVIEHRALSTSALA----HGRALGLTSESRVLqfasytFDVSILEIFTTLAAGGC--------- 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 mnLLIPNPRD-VDGFVKELSRYRVNFfpgvntlfnALLNhPGFAAL----DFSGLRiTLG-GGMAVQEVVSTRWMKttGC 354
Cdd:cd05918 175 --LCIPSEEDrLNDLAGFINRLRVTW---------AFLT-PSVARLldpeDVPSLR-TLVlGGEALTQSDVDTWAD--RV 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 PILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTD-IALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKV-- 431
Cdd:cd05918 240 RLINAYGPAECTIAATVSPVVPSTDPRNIGRPLGATCwVVDPDNHDRLVPIGAVGELLIEGPILARGYLNDPEKTAAAfi 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 432 ---------MTPDG--FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEA 500
Cdd:cd05918 320 edpawlkqeGSGRGrrLYRTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSS 399
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 501 VKL---FVVRRDPALTT------------------EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:cd05918 400 SPQlvaFVVLDGSSSGSgdgdslflepsdefralvAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRALRE 475
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
49-560 |
1.60e-34 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 138.63 E-value: 1.60e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAeAIVVLE 128
Cdd:PRK06060 31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEP-ALVVTS 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 nfattlqqviartdvrhvvvaslgdllGPVRgmavnfvarrvKKLVPPYRLPGATPFRTALRDGarqPFQPAALNHDDVA 208
Cdd:PRK06060 110 ---------------------------DALR-----------DRFQPSRVAEAAELMSEAARVA---PGGYEPMGGDALA 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKAPQIdalVTVCALPLYHILALNACMLMGARWGAMNLLIPNP 288
Cdd:PRK06060 149 YATYTSGTTGPPKAAIHRHADPLTFV---DAMCRKALRLTPED---TGLCSARMYFAYGLGNSVWFPLATGGSAVINSAP 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 289 RDVDGFVKELSRYRVNFFPGVNTLFNALLNhpGFAALDFSGLRITLGGGMAVQEVVSTRWMKT-TGCPILEGYGLSETSP 367
Cdd:PRK06060 223 VTPEAAAILSARFGPSVLYGVPNFFARVID--SCSPDSFRSLRCVVSAGEALELGLAERLMEFfGGIPILDGIGSTEVGQ 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 368 svTLSPPTLGAF-TGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDetaKVMTPDGFFKSGDVGVM 446
Cdd:PRK06060 301 --TFVSNRVDEWrLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPD---SPVANEGWLDTRDRVCI 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 447 DEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKK--- 523
Cdd:PRK06060 376 DSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRgll 455
|
490 500 510
....*....|....*....|....*....|....*...
gi 979216869 524 -EFTGYKKPKYIEFRDSLPKSNIGKILRRELRdehGQT 560
Cdd:PRK06060 456 nRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR---KQS 490
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
51-556 |
5.44e-34 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 135.74 E-value: 5.44e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 51 YAELDTYSR--HLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLE 128
Cdd:PLN02479 46 YTWAQTYQRcrRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVDQ 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 NFATTLQQVI------ARTDVRHVVVASLGDLLGPVRGMavNFVARRvkklvppyrlpGATPFRTALRDGARQ-PFQPAA 201
Cdd:PLN02479 126 EFFTLAEEALkilaekKKSSFKPPLLIVIGDPTCDPKSL--QYALGK-----------GAIEYEKFLETGDPEfAWKPPA 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 202 LNHDDVAfLQYTGGTTGVSKGATLTHRNVIANILQND-AWSQPalrkapqiDALVTVCALPLYHilalnaCMLMGARWGA 280
Cdd:PLN02479 193 DEWQSIA-LGYTSGTTASPKGVVLHHRGAYLMALSNAlIWGMN--------EGAVYLWTLPMFH------CNGWCFTWTL 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 MNLLIPNP--RDVD--GFVKELSRYRVNFFPGVNTLFNALLNHPGF-AALDFSGLRITLGGGMAVQEVVSTRwMKTTGCP 355
Cdd:PLN02479 258 AALCGTNIclRQVTakAIYSAIANYGVTHFCAAPVVLNTIVNAPKSeTILPLPRVVHVMTAGAAPPPSVLFA-MSEKGFR 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 356 ILEGYGLSET-SPSVTLS--------PPTLGA---------FTGTIGLPVPSTdiallgENDEPV-AQGQP-GEIAIRGP 415
Cdd:PLN02479 337 VTHTYGLSETyGPSTVCAwkpewdslPPEEQArlnarqgvrYIGLEGLDVVDT------KTMKPVpADGKTmGEIVMRGN 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 416 QVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDS 495
Cdd:PLN02479 411 MVMKGYLKNPKANEEAFA-NGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDE 489
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 496 NSGEAVKLFVV------RRDPALTTEALMAYCKKEFTGYKKPKYIEFrDSLPKSNIGKILRRELRDE 556
Cdd:PLN02479 490 RWGESPCAFVTlkpgvdKSDEAALAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKHVLRAK 555
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
47-553 |
5.70e-34 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 134.13 E-value: 5.70e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:cd17650 11 RQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfQPaalnhDD 206
Cdd:cd17650 91 -----------------------------------------------------------------------QP-----ED 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 207 VAFLQYTGGTTGVSKGATLTHRNVIANILqndAWsqpalRKAPQIDALvTVCALPLYHI--------LALNACmlmgarw 278
Cdd:cd17650 95 LAYVIYTSGTTGKPKGVMVEHRNVAHAAH---AW-----RREYELDSF-PVRLLQMASFsfdvfagdFARSLL------- 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 gAMNLLIPNPRDV----DGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGmavqEVVSTRWMKT--- 351
Cdd:cd17650 159 -NGGTLVICPDEVkldpAALYDLILKSRITLMESTPALIRPVMAYVYRNGLDLSAMRLLIVGS----DGCKAQDFKTlaa 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 352 ---TGCPILEGYGLSETSPSVTLSPPTLGAFTGT----IGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNR 424
Cdd:cd17650 234 rfgQGMRIINSYGVTEATIDSTYYEEGRDPLGDSanvpIGRPLPNTAMYVLDERLQPQPVGVAGELYIGGAGVARGYLNR 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 425 PDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSG 498
Cdd:cd17650 314 PELTAERFVENPFapgermYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGE 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 499 EAVKLFVVRRDpALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17650 394 ARLCAYVVAAA-TLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
18-553 |
1.17e-33 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 137.40 E-value: 1.17e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 18 DPDRYP---SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK12316 503 TAAEYPlqrGVHRLFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAIL 582
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVNVNPLYTPRELEHQLRDSGAEAIvvlenfattLQQviartdvRHvvvasLGDLLGpvrgmavnfVARRVKKLV 174
Cdd:PRK12316 583 KAGGAYVPLDPEYPAERLAYMLEDSGVQLL---------LSQ-------SH-----LGRKLP---------LAAGVQVLD 632
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 175 ppyrLPGATPFRTALRDGArqPFQpaALNHDDVAFLQYTGGTTGVSKGATLTHRNViANILQndaWSQPA---------L 245
Cdd:PRK12316 633 ----LDRPAAWLEGYSEEN--PGT--ELNPENLAYVIYTSGSTGKPKGAGNRHRAL-SNRLC---WMQQAyglgvgdtvL 700
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 246 RKAP-QIDALVTVCALPLyhilalnacmLMGARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPG 321
Cdd:PRK12316 701 QKTPfSFDVSVWEFFWPL----------MSGAR-----LVVAAPgdhRDPAKLVELINREGVDTLHFVPSMLQAFLQDED 765
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 322 FAALDfSGLRITLGG----GMAVQEVVSTRWMkttgCPILEGYGLSETSPSVT-LSPPTLGAFTGTIGLPVPSTDIALLG 396
Cdd:PRK12316 766 VASCT-SLRRIVCSGealpADAQEQVFAKLPQ----AGLYNLYGPTEAAIDVThWTCVEEGGDSVPIGRPIANLACYILD 840
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 397 ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVY 470
Cdd:PRK12316 841 ANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFvagermYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIE 920
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 471 PNEIESVVAAHPGVIECACVGVpdsnSGEAVKLFVVRRDPALTT-EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKIL 549
Cdd:PRK12316 921 LGEIEARLLEHPWVREAAVLAV----DGKQLVGYVVLESEGGDWrEALKAHLAASLPEYMVPAQWLALERLPLTPNGKLD 996
|
....
gi 979216869 550 RREL 553
Cdd:PRK12316 997 RKAL 1000
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
45-555 |
1.49e-33 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 135.00 E-value: 1.49e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 45 MGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAG--FTVVNVNplYTPRELEHQLRDSGAE 122
Cdd:cd05966 81 QSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGavHSVVFAG--FSAESLADRINDAQCK 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 123 AIVVLENF-----ATTLQQVI-----ARTDVRHVVVAslgdllgpvrgmavnfvaRRVKKLVPPYrlPGATPFRTALRDG 192
Cdd:cd05966 159 LVITADGGyrggkVIPLKEIVdealeKCPSVEKVLVV------------------KRTGGEVPMT--EGRDLWWHDLMAK 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 193 ARQPFQPAALNHDDVAFLQYTGGTTGVSKG-----------ATLTHRNVIaNILQND--------AWsqpalrkapqida 253
Cdd:cd05966 219 QSPECEPEWMDSEDPLFILYTSGSTGKPKGvvhttggyllyAATTFKYVF-DYHPDDiywctadiGW------------- 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 lVTVCALPLYHILALNACMLM--GArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALL----NHPgfAALDF 327
Cdd:cd05966 285 -ITGHSYIVYGPLANGATTVMfeGT---------PTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMkfgdEWV--KKHDL 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 328 SGLRItLGggmAVQEVVSTR-WM---KTTG---CPILEGYGLSETSpSVTLSP-PtlGAFT---GTIGLPVPSTDIALLG 396
Cdd:cd05966 353 SSLRV-LG---SVGEPINPEaWMwyyEVIGkerCPIVDTWWQTETG-GIMITPlP--GATPlkpGSATRPFFGIEPAILD 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 397 ENDEPVAQGQPGEIAIRG--PQVMAGYWNRPDETAKVMTPD--GFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPN 472
Cdd:cd05966 426 EEGNEVEGEVEGYLVIKRpwPGMARTIYGDHERYEDTYFSKfpGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTA 505
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 473 EIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRD-----PALTTEaLMAYCKKEFTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:cd05966 506 EVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDgeepsDELRKE-LRKHVRKEIGPIATPDKIQFVPGLPKTRSGK 584
|
....*...
gi 979216869 548 ILRRELRD 555
Cdd:cd05966 585 IMRRILRK 592
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
49-553 |
2.31e-33 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 133.98 E-value: 2.31e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLE 128
Cdd:PRK05857 42 LRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALVAP 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 NfattlqqviartdvRHVVVASLGDLLGPVRGMAVNFVArrvkklvppyrlpGATPFRTALrDGARQPFQPAaLNHDDVA 208
Cdd:PRK05857 122 G--------------SKMASSAVPEALHSIPVIAVDIAA-------------VTRESEHSL-DAASLAGNAD-QGSEDPL 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRNVIA--NILQNDAwsqpaLRKAPQIDALVTVCALPLYHILAL---NACMLMGArwgamnL 283
Cdd:PRK05857 173 AMIFTSGTTGEPKAVLLANRTFFAvpDILQKEG-----LNWVTWVVGETTYSPLPATHIGGLwwiLTCLMHGG------L 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR-ITLGGGMAVQEVVstRWMKTTGCPILEGYGL 362
Cdd:PRK05857 242 CVTGGENTTSLLEILTTNAVATTCLVPTLLSKLVSELKSANATVPSLRlVGYGGSRAIAADV--RFIEATGVRTAQVYGL 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 363 SETSPSVTLSPPTLGAFT----GTIGLPVPSTDIALLGEN--DEPVAQGQP----GEIAIRGPQVMAGYWNRPDETAKVM 432
Cdd:PRK05857 320 SETGCTALCLPTDDGSIVkieaGAVGRPYPGVDVYLAATDgiGPTAPGAGPsasfGTLWIKSPANMLGYWNNPERTAEVL 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 TpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRR---D 509
Cdd:PRK05857 400 I-DGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVASaelD 478
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 979216869 510 PALTTE---ALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK05857 479 ESAARAlkhTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
47-520 |
2.72e-33 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 133.49 E-value: 2.72e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVv 126
Cdd:PRK09274 40 DELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVPVLVDPGMGIKNLKQCLAEAQPDAFI- 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfATTLQQVIART------DVRHVVVaslgdllgpvrgmavnfVARRVkklvppyrLPGATPFRTALRDGARQPFQPA 200
Cdd:PRK09274 119 ----GIPKAHLARRLfgwgkpSVRRLVT-----------------VGGRL--------LWGGTTLATLLRDGAAAPFPMA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 201 ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANI--LQNDAWSQPalrkaPQIDalvtvcaLPLYHILAL-NACMLMGAR 277
Cdd:PRK09274 170 DLAPDDMAAILFTSGSTGTPKGVVYTHGMFEAQIeaLREDYGIEP-----GEID-------LPTFPLFALfGPALGMTSV 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 278 WGAMNLLIP---NPRDVdgfVKELSRYRV-NFFpGVNTLFNALLNHPGFAALDFSGLRITLGGG----MAVQEVVSTrwM 349
Cdd:PRK09274 238 IPDMDPTRPatvDPAKL---FAAIERYGVtNLF-GSPALLERLGRYGEANGIKLPSLRRVISAGapvpIAVIERFRA--M 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPILEGYGLSETSPSVTL-SPPTLGAFT-------GT-IGLPVPSTDIALLGENDEP---------VAQGQPGEIA 411
Cdd:PRK09274 312 LPPDAEILTPYGATEALPISSIeSREILFATRaatdngaGIcVGRPVDGVEVRIIAISDAPipewddalrLATGEIGEIV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYWNRPDET--AKVMTPDGFF--KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEC 487
Cdd:PRK09274 392 VAGPMVTRSYYNRPEATrlAKIPDGQGDVwhRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRS 471
|
490 500 510
....*....|....*....|....*....|...
gi 979216869 488 ACVGVPDsnSGEAVKLFVVRRDPALTTEALMAY 520
Cdd:PRK09274 472 ALVGVGV--PGAQRPVLCVELEPGVACSKSALY 502
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
28-553 |
3.53e-33 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 132.45 E-value: 3.53e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 28 LLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLY 107
Cdd:cd17655 2 LFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 108 TPRELEHQLRDSGAEAIVvlenfatTLQQVIARTDVRHVVVASLGDllgpvrgmavNFVARRVKKLVPPyrlpgatpfrt 187
Cdd:cd17655 82 PEERIQYILEDSGADILL-------TQSHLQPPIAFIGLIDLLDED----------TIYHEESENLEPV----------- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 188 alrdgarqpfqpaaLNHDDVAFLQYTGGTTGVSKGATLTHRNVI------ANILQNDAWSQPALRKAPQIDALVTvcalP 261
Cdd:cd17655 134 --------------SKSDDLAYVIYTSGSTGKPKGVMIEHRGVVnlvewaNKVIYQGEHLRVALFASISFDASVT----E 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 262 LYHILALnacmlmgarwGAMNLLIPNPRDVDG--FVKELSRYRVNFFPGVNTLFNaLLNHPGfaALDFSGLRITLGGGMA 339
Cdd:cd17655 196 IFASLLS----------GNTLYIVRKETVLDGqaLTQYIRQNRITIIDLTPAHLK-LLDAAD--DSEGLSLKHLIVGGEA 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 340 VQEVVSTRWMK--TTGCPILEGYGLSETSPSVTLSPPTLGAFTGT---IGLPVPSTDIALLGENDEPVAQGQPGEIAIRG 414
Cdd:cd17655 263 LSTELAKKIIElfGTNPTITNAYGPTETTVDASIYQYEPETDQQVsvpIGKPLGNTRIYILDQYGRPQPVGVAGELYIGG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 415 PQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECA 488
Cdd:cd17655 343 EGVARGYLNRPELTAEKFVDDPFvpgermYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAV 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 489 CVGVPDSNSGEAVKLFVVrRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17655 423 VIARKDEQGQNYLCAYIV-SEKELPVAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
46-556 |
6.48e-33 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 130.94 E-value: 6.48e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 Vlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpaalnhd 205
Cdd:cd05940 81 V------------------------------------------------------------------------------- 81
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNvianILQNDAWSQPALRKAPQiDALVTvcALPLYH----ILALNACMLMGArwgam 281
Cdd:cd05940 82 DAALYIYTSGTTGLPKAAIISHRR----AWRGGAFFAGSGGALPS-DVLYT--CLPLYHstalIVGWSACLASGA----- 149
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGM--AVQEVVSTRWmkttGCP-ILE 358
Cdd:cd05940 150 TLVIRKKFSASNFWDDIRKYQATIFQYIGELCRYLLNQPPKPTERKHKVRMIFGNGLrpDIWEEFKERF----GVPrIAE 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTlsppTLGAFTGTIG----LPVPSTDIALLG---ENDEP----------VAQGQPGEI--AIRGPQVMA 419
Cdd:cd05940 226 FYAATEGNSGFI----NFFGKPGAIGrnpsLLRKVAPLALVKydlESGEPirdaegrcikVPRGEPGLLisRINPLEPFD 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 GYWNRPDETAKVMTP-----DGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVP- 493
Cdd:cd05940 302 GYTDPAATEKKILRDvfkkgDAWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQv 381
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 494 ---DSNSGEAVklFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:cd05940 382 pgtDGRAGMAA--IVLQPNEEFDLSALAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRNE 445
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
47-555 |
1.02e-32 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 132.82 E-value: 1.02e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAEL-DTYSRhLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAeAIV 125
Cdd:cd05967 81 RTYTYAELlDEVSR-LAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKP-KLI 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATTLQQVI------------ARTDVRHVVVASLGDL-LGPVRgmavnfvarrvkklvppyrlPGATPFRTALRDG 192
Cdd:cd05967 159 VTASCGIEPGKVVpykplldkalelSGHKPHHVLVLNRPQVpADLTK--------------------PGRDLDWSELLAK 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 193 ArQPFQPAALNHDDVAFLQYTGGTTGVSKGA-----------TLTHRNvIANILQNDAWSQpalrkAPQIDALVT---VC 258
Cdd:cd05967 219 A-EPVDCVPVAATDPLYILYTSGTTGKPKGVvrdngghavalNWSMRN-IYGIKPGDVWWA-----ASDVGWVVGhsyIV 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 259 ALPLYHilalnacmlmgarwGAMNLL-----IPNPrDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAAL----DFSG 329
Cdd:cd05967 292 YGPLLH--------------GATTVLyegkpVGTP-DPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPDGKYikkyDLSS 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 330 LR-ITLGGGMAVQEVVStrWM-KTTGCPILEGYGLSETSPSVTLSPPTLGAF---TGTIGLPVPSTDIALLGENDEPVAQ 404
Cdd:cd05967 357 LRtLFLAGERLDPPTLE--WAeNTLGVPVIDHWWQTETGWPITANPVGLEPLpikAGSPGKPVPGYQVQVLDEDGEPVGP 434
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 405 GQPGEIAIRGP---QVMAGYWNRPD--ETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVA 479
Cdd:cd05967 435 NELGNIVIKLPlppGCLLTLWKNDErfKKLYLSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVL 514
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 480 AHPGVIECACVGVPDSNSGEAVKLFVV-----RRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05967 515 SHPAVAECAVVGVRDELKGQVPLGLVVlkegvKITAEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLR 594
|
.
gi 979216869 555 D 555
Cdd:cd05967 595 K 595
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
41-553 |
2.78e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 129.70 E-value: 2.78e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 41 AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSG 120
Cdd:cd12114 5 AVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAILADAG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 121 AEAIVVlenfattlQQVIARTDVRHVVVASLGDLLgpvrgmavnfvarrvkklvppyrlpgatpfrtalrDGARQPFQPA 200
Cdd:cd12114 85 ARLVLT--------DGPDAQLDVAVFDVLILDLDA-----------------------------------LAAPAPPPPV 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 201 ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQ-NDAWsqpALRKAPQIDALVTVC-ALPLYHIL-ALNAcmlmgar 277
Cdd:cd12114 122 DVAPDDLAYVIFTSGSTGTPKGVMISHRAALNTILDiNRRF---AVGPDDRVLALSSLSfDLSVYDIFgALSA------- 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 278 wGAmNLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTT-G 353
Cdd:cd12114 192 -GA-TLVLPDEarrRDPAHWAELIERHGVTLWNSVPALLEMLLDVLEAAQALLPSLRLVLLSGDWIPLDLPARLRALApD 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 354 CPILEGYGLSETSP-SVTLSPPTLGAFTGTI--GLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAK 430
Cdd:cd12114 270 ARLISLGGATEASIwSIYHPIDEVPPDWRSIpyGRPLANQRYRVLDPRGRDCPDWVPGELWIGGRGVALGYLGDPELTAA 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 431 ----VMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVpDSNSGEAVKLFVV 506
Cdd:cd12114 350 rfvtHPDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVV 428
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 979216869 507 RRDPA--LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd12114 429 PDNDGtpIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
69-554 |
4.74e-32 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 130.29 E-value: 4.74e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 69 GLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTD-VRHVV 147
Cdd:PRK05620 60 GITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVADPRLAEQLGEILKECPcVRAVV 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 148 VASLGDLLGPVRGMAVNFVARRVKklvppyrlpgatpfrtALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTH 227
Cdd:PRK05620 140 FIGPSDADSAAAHMPEGIKVYSYE----------------ALLDGRSTVYDWPELDETTAAAICYSTGTTGAPKGVVYSH 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 228 RNVIANILQndawsqpaLRKApqiDAL-VT-----VCALPLYHILALnaCMLMGARWGAMNLLIPNPrDVDG----FVKE 297
Cdd:PRK05620 204 RSLYLQSLS--------LRTT---DSLaVThgesfLCCVPIYHVLSW--GVPLAAFMSGTPLVFPGP-DLSAptlaKIIA 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 298 LSRYRVNFfpGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLG 377
Cdd:PRK05620 270 TAMPRVAH--GVPTLWIQLMVHYLKNPPERMSLQEIYVGGSAVPPILIKAWEERYGVDVVHVWGMTETSPVGTVARPPSG 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 378 A--------------FTGTIGLPVpSTDIALLGENDEpvaqgQPGEIAIRGPQVMAGYWNRP----------------DE 427
Cdd:PRK05620 348 VsgearwayrvsqgrFPASLEYRI-VNDGQVMESTDR-----NEGEIQVRGNWVTASYYHSPteegggaastfrgedvED 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 428 TAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGE---AVKLF 504
Cdd:PRK05620 422 ANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGErplAVTVL 501
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 505 VVRRDP-ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK05620 502 APGIEPtRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLR 552
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
46-561 |
8.81e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 129.03 E-value: 8.81e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGAR-VAVMMPNVLQYPVAVAAALRAGFTVVNVNPlyTPRelehqlrdsGAEai 124
Cdd:PRK07867 26 DSFTSWREHIRGSAARAAALRARLDPTRPPhVGVLLDNTPEFSLLLGAAALSGIVPVGLNP--TRR---------GAA-- 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 vvlenfattLQQVIARTDVRHVV-----VASLGDLLGPVRgmAVNFVARRVKKLVPPYRlpgatpfrtalrdGARQPFQP 199
Cdd:PRK07867 93 ---------LARDIAHADCQLVLtesahAELLDGLDPGVR--VINVDSPAWADELAAHR-------------DAEPPFRV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 200 AALnhDDVAFLQYTGGTTGVSKGATLTHRNVianilqndAWSQPALRKAPQIDAL-VTVCALPLYHILALNACMLMGARW 278
Cdd:PRK07867 149 ADP--DDLFMLIFTSGTSGDPKAVRCTHRKV--------ASAGVMLAQRFGLGPDdVCYVSMPLFHSNAVMAGWAVALAA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GAmNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVvsTRWMKTTGCPILE 358
Cdd:PRK07867 219 GA-SIALRRKFSASGFLPDVRRYGATYANYVGKPLSYVLATPERPDDADNPLRIVYGNEGAPGDI--ARFARRFGCVVVD 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSPSVTLSPPTLgafTGTIGLPVPSTDI-----------ALLGENDEPVAQGQPGEIA-IRGPQVMAGYWNRPD 426
Cdd:PRK07867 296 GFGSTEGGVAITRTPDTP---PGALGPLPPGVAIvdpdtgtecppAEDADGRLLNADEAIGELVnTAGPGGFEGYYNDPE 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 427 ETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV 506
Cdd:PRK07867 373 ADAERMR-GGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALV 451
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 507 RR-----DPALTTEALMAycKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHGQTA 561
Cdd:PRK07867 452 LApgakfDPDAFAEFLAA--QPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSAEGVDCA 509
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
19-553 |
2.16e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 130.46 E-value: 2.16e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 19 PDRYPSlAHLLDESFATFAARNAYACM----GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK12316 4544 DAGYPA-TRCVHQLVAERARMTPDAVAvvfdEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVL 4622
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVNVNPLYtPRE-LEHQLRDSGAEaivVLENFATTLQQVIARTDVRHVVVASLGDLLGpvrgmavnfvarrvkkl 173
Cdd:PRK12316 4623 KAGGAYVPLDPEY-PRErLAYMMEDSGAA---LLLTQSHLLQRLPIPDGLASLALDRDEDWEG----------------- 4681
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 174 vppyrLPGATPFRtalrdgarqpfqpaALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndAWSQPALRKAPQIDA 253
Cdd:PRK12316 4682 -----FPAHDPAV--------------RLHPDNLAYVIYTSGSTGRPKGVAVSHGSLVNHL----HATGERYELTPDDRV 4738
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 L------VTVCALPLYHILALNACMLMGArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGfAALDF 327
Cdd:PRK12316 4739 LqfmsfsFDGSHEGLYHPLINGASVVIRD---------DSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAE-RDGEP 4808
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 328 SGLRITLGGGMAVQEVVSTR-WMKTTGCPILEGYGLSETSPSVTL----SPPTLGAFTGTIGLPVPSTDIALLGENDEPV 402
Cdd:PRK12316 4809 PSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTVTVLLwkarDGDACGAAYMPIGTPLGNRSGYVLDGQLNPL 4888
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 AQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIE 475
Cdd:PRK12316 4889 PVGVAGELYLGGEGVARGYLERPALTAERFVPDPFgapggrlYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIE 4968
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 476 SVVAAHPGVIECACVGVPDSNSGEAVKlFVVRRDPALTT---------EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIG 546
Cdd:PRK12316 4969 ARLREHPAVREAVVIAQEGAVGKQLVG-YVVPQDPALADadeaqaelrDELKAALRERLPEYMVPAHLVFLARMPLTPNG 5047
|
....*..
gi 979216869 547 KILRREL 553
Cdd:PRK12316 5048 KLDRKAL 5054
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
19-553 |
3.96e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 129.69 E-value: 3.96e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 19 PDRYPSlAHLLDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK12316 1996 PEAYPR-GPGVHQRIAEQAARApeaiAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVL 2074
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVNVNPLYtPRE-LEHQLRDSGAeaivvlenfattlqqviartdvrhvvvaslgDLLGPVRGMAvnfvarrvKKL 173
Cdd:PRK12316 2075 KAGGAYVPLDPNY-PAErLAYMLEDSGA-------------------------------ALLLTQRHLL--------ERL 2114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 174 VPPYRLPgATPFRTALRDGARQPFQPA-ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQ--PA---LRK 247
Cdd:PRK12316 2115 PLPAGVA-RLPLDRDAEWADYPDTAPAvQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYElsPAdceLQF 2193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 248 AP-QIDALVTVCALPLYHilalnacmlmGARwgamnLLIPNP--RDVDGFVKELSRYRVNF--FPGVntLFNALLNHPGF 322
Cdd:PRK12316 2194 MSfSFDGAHEQWFHPLLN----------GAR-----VLIRDDelWDPEQLYDEMERHGVTIldFPPV--YLQQLAEHAER 2256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 323 AALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTL----SPPTLGAFTGTIGLPVPSTDIALLGEN 398
Cdd:PRK12316 2257 DGRPPAVRVYCFGGEAVPAASLRLAWEALRPVYLFNGYGPTEAVVTPLLwkcrPQDPCGAAYVPIGRALGNRRAYILDAD 2336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 399 DEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYP 471
Cdd:PRK12316 2337 LNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIEL 2416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 472 NEIESVVAAHPGVIECACVGVpDSNSGEAVKLFVVRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:PRK12316 2417 GEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDDAAeDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDR 2495
|
...
gi 979216869 551 REL 553
Cdd:PRK12316 2496 KAL 2498
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
27-553 |
1.66e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 127.58 E-value: 1.66e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 27 HLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPL 106
Cdd:PRK12467 516 QLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPE 595
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 107 YtPRE-LEHQLRDSGAEAIVVLENfatTLQQVIARTDVRHVVVASLGDLLGpvrgmavnfvarrvkklvppyRLPGATPf 185
Cdd:PRK12467 596 Y-PQDrLAYMLDDSGVRLLLTQSH---LLAQLPVPAGLRSLCLDEPADLLC---------------------GYSGHNP- 649
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 186 rtalrdgarqpfqPAALNHDDVAFLQYTGGTTGVSKGATLTHRNvIANILQndaWSQPALRKAPqID--ALVTVCALPLY 263
Cdd:PRK12467 650 -------------EVALDPDNLAYVIYTSGSTGQPKGVAISHGA-LANYVC---VIAERLQLAA-DDsmLMVSTFAFDLG 711
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 264 HILALNACMLmgarwGAMNLLIP--NPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALdfSGLRITLGGGMAVQ 341
Cdd:PRK12467 712 VTELFGALAS-----GATLHLLPpdCARDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALP--RPQRALVCGGEALQ 784
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 342 EVVSTRWM-KTTGCPILEGYGLSETSPSVT---LSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQV 417
Cdd:PRK12467 785 VDLLARVRaLGPGARLINHYGPTETTVGVStyeLSDEERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGL 864
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 418 MAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACV 490
Cdd:PRK12467 865 ARGYHRRPALTAERFVPDPFgadggrlYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVL 944
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 491 GVPDSNSGEAVKLFVVRR-----DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK12467 945 AQPGDAGLQLVAYLVPAAvadgaEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
19-553 |
1.69e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 127.58 E-value: 1.69e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 19 PDRYPsLAHLLDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK12467 1567 HTGYP-LARLVHQLIEDQAAATpeavALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAIL 1645
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVNVNPLYTPRELEHQLRDSGAEaivVLENFATTLQQVIARTDVRHVVVASLGDLLGpvrgmavnfvarrvkklv 174
Cdd:PRK12467 1646 KAGGAYVPLDPEYPRERLAYMIEDSGIE---LLLTQSHLQARLPLPDGLRSLVLDQEDDWLE------------------ 1704
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 175 ppyrlpgatpfrtalrdgARQPFQPA-ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILqndawsqpALRKAPQIDA 253
Cdd:PRK12467 1705 ------------------GYSDSNPAvNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLC--------ATQEAYQLSA 1758
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 LVTVCalpLYHILALNACM--LMGARWGAMNLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFS 328
Cdd:PRK12467 1759 ADVVL---QFTSFAFDVSVweLFWPLINGARLVIAPPgahRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLS 1835
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 329 gLRITLGGGMAVQEVVSTRWMKTTG-CPILEGYGLSETSPSVTLSPPTLGAFTGT----IGLPVPSTDIALLGENDEPVA 403
Cdd:PRK12467 1836 -LRRVVCGGEALEVEALRPWLERLPdTGLFNLYGPTETAVDVTHWTCRRKDLEGRdsvpIGQPIANLSTYILDASLNPVP 1914
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 404 QGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIES 476
Cdd:PRK12467 1915 IGVAGELYLGGVGLARGYLNRPALTAERFVADPFgtvgsrlYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEA 1994
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 477 VVAAHPGVIECACVGVPDSNSGEAVKlFVVRRDPALTTEA---------LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:PRK12467 1995 RLREQGGVREAVVIAQDGANGKQLVA-YVVPTDPGLVDDDeaqvalraiLKNHLKASLPEYMVPAHLVFLARMPLTPNGK 2073
|
....*.
gi 979216869 548 ILRREL 553
Cdd:PRK12467 2074 LDRKAL 2079
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
6-442 |
2.26e-30 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 125.76 E-value: 2.26e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 6 LKSYPEGVP------ATVDPDRypslahlldesfaTFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVM 79
Cdd:PRK08180 34 LGDYPRRLTdrlvhwAQEAPDR-------------VFLAERGADGGWRRLTYAEALERVRAIAQALLDRGLSAERPLMIL 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 80 MPNVLQYPVAVAAALRAGFTVVNVNPLYTprelehqlrdsgaeaiVVLENFATtLQQVIARTDVRHVVV---ASLGDLLG 156
Cdd:PRK08180 101 SGNSIEHALLALAAMYAGVPYAPVSPAYS----------------LVSQDFGK-LRHVLELLTPGLVFAddgAAFARALA 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 157 PVRGMAVNFVARRvkklvPPYRLPGATPFRT--ALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANI 234
Cdd:PRK08180 164 AVVPADVEVVAVR-----GAVPGRAATPFAAllATPPTAAVDAAHAAVGPDTIAKFLFTSGSTGLPKAVINTHRMLCANQ 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 235 LQ-NDAWsqPALRKAPQidalVTVCALPLYHILALNACMLMGARWGAmNLLI----PNPRDVDGFVKELSRYRVNFFPGV 309
Cdd:PRK08180 239 QMlAQTF--PFLAEEPP----VLVDWLPWNHTFGGNHNLGIVLYNGG-TLYIddgkPTPGGFDETLRNLREISPTVYFNV 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 310 NTLFNALLNH----PGFAALDFSGLRITLGGGMAVQEVVSTRW----MKTTG--CPILEGYGLSETSPSVT-LSPPTLGA 378
Cdd:PRK08180 312 PKGWEMLVPAlerdAALRRRFFSRLKLLFYAGAALSQDVWDRLdrvaEATCGerIRMMTGLGMTETAPSATfTTGPLSRA 391
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 979216869 379 ftGTIGLPVPSTDIALlgendepVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGD 442
Cdd:PRK08180 392 --GNIGLPAPGCEVKL-------VPVGGKLEVRVKGPNVTPGYWRAPELTAEAFDEEGYYRSGD 446
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
46-556 |
4.43e-30 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 123.98 E-value: 4.43e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAwlQLRGLRKGAR---VAVMMPNVLQYPVAVAAALRAGFTVVNVNPlyTPRelehqlrdsGAE 122
Cdd:PRK13388 24 DRTWTWREVLAEAAARAA--ALIALADPDRplhVGVLLGNTPEMLFWLAAAALGGYVLVGLNT--TRR---------GAA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 123 aivvlenfattLQQVIARTDVRHVVVASLGdlLGPVRGMAVNFVaRRVKKLVPPYR--LPGATPFRTALRDGARQPFqpa 200
Cdd:PRK13388 91 -----------LAADIRRADCQLLVTDAEH--RPLLDGLDLPGV-RVLDVDTPAYAelVAAAGALTPHREVDAMDPF--- 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 201 alnhddvaFLQYTGGTTGVSKGATLTHRNVIANilqndawSQPALRKAPQIDALVTVCALPLYH----------ILALNA 270
Cdd:PRK13388 154 --------MLIFTSGTTGAPKAVRCSHGRLAFA-------GRALTERFGLTRDDVCYVSMPLFHsnavmagwapAVASGA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 271 CMLMGARWGAmnllipnprdvDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVvsTRWMK 350
Cdd:PRK13388 219 AVALPAKFSA-----------SGFLDDVRRYGATYFNYVGKPLAYILATPERPDDADNPLRVAFGNEASPRDI--AEFSR 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 351 TTGCPILEGYGLSETSPSVTLSPptlGAFTGTIGLPVPSTDI-----------ALLGEN------DEPVaqgqpGEIA-I 412
Cdd:PRK13388 286 RFGCQVEDGYGSSEGAVIVVREP---GTPPGSIGRGAPGVAIynpetltecavARFDAHgallnaDEAI-----GELVnT 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 413 RGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGV 492
Cdd:PRK13388 358 AGAGFFEGYYNNPEATAERMR-HGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAV 436
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 493 PDSNSGEAVKLFVVRRDPA-LTTEALMAY-CKKEFTGYK-KPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK13388 437 PDERVGDQVMAALVLRDGAtFDPDAFAAFlAAQPDLGTKaWPRYVRIAADLPSTATNKVLKRELIAQ 503
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
4-556 |
1.28e-29 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 123.06 E-value: 1.28e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 4 IWLKSYPEGVPATVDPDRypSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNV 83
Cdd:PRK08279 20 GILRGLKRTALITPDSKR--SLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENR 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 84 LQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDvRHVVVASLGDLLGPVRGMAV 163
Cdd:PRK08279 98 PEYLAAWLGLAKLGAVVALLNTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLA-RPPRLWVAGGDTLDDPEGYE 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 164 NFVArrvkklvppyRLPGATPFRTALRDGarqpfqpaaLNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndAWSQP 243
Cdd:PRK08279 177 DLAA----------AAAGAPTTNPASRSG---------VTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAM----GGFGG 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 244 ALRKAPQiDalVTVCALPLYHILALNAC---MLMGArwGAMNLlipnpRD---VDGFVKELSRYRVNFFPGVNTLFNALL 317
Cdd:PRK08279 234 LLRLTPD-D--VLYCCLPLYHNTGGTVAwssVLAAG--ATLAL-----RRkfsASRFWDDVRRYRATAFQYIGELCRYLL 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 318 NHPGfAALDFS-GLRITLGGGM--AVQEVVSTRWmkttGCP-ILEGYGLSETSPSVTlsppTLGAFTGTIGLpVPS---T 390
Cdd:PRK08279 304 NQPP-KPTDRDhRLRLMIGNGLrpDIWDEFQQRF----GIPrILEFYAASEGNVGFI----NVFNFDGTVGR-VPLwlaH 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 391 DIALL---GENDEP----------VAQGQPGE----IAIRGPqvMAGYwNRPDETAKVMTPDGF------FKSGDVGVMD 447
Cdd:PRK08279 374 PYAIVkydVDTGEPvrdadgrcikVKPGEVGLligrITDRGP--FDGY-TDPEASEKKILRDVFkkgdawFNTGDLMRDD 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 448 EHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVP----DSNSGEAVklFVVRRDPALTTEALMAYCKK 523
Cdd:PRK08279 451 GFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGVEEAVVYGVEvpgtDGRAGMAA--IVLADGAEFDLAALAAHLYE 528
|
570 580 590
....*....|....*....|....*....|...
gi 979216869 524 EFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK08279 529 RLPAYAVPLFVRLVPELETTGTFKYRKVDLRKE 561
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
46-484 |
1.69e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 122.41 E-value: 1.69e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRH-----------LGAWLQLRGLRKGARVAVM--MPnVLQYPVAVAAALRAGFTVVNVNPlyTPR-E 111
Cdd:PRK07768 16 PRGMVTGEPDAPVRHtwgevherarrIAGGLAAAGVGPGDAVAVLagAP-VEIAPTAQGLWMRGASLTMLHQP--TPRtD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 112 LEHQLRDSGAeaivvlenfattlqqVIARTDVRHVVVASLGDLLGPV---RGMAVNFVARrvkklvppyrlpgatpfrta 188
Cdd:PRK07768 93 LAVWAEDTLR---------------VIGMIGAKAVVVGEPFLAAAPVleeKGIRVLTVAD-------------------- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 189 LRDGArqPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqPALRKAPQIDAL--VTVCALPLYHIL 266
Cdd:PRK07768 138 LLAAD--PIDPVETGEDDLALMQLTSGSTGSPKAVQITHGNLYANA--------EAMFVAAEFDVEtdVMVSWLPLFHDM 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 267 ALNAC----MLMGarwgaMNLLIPNP----RDVDGFVKELSRYRVNFFPGVNTLFNA----LLNHPGFAALDFSGLRITL 334
Cdd:PRK07768 208 GMVGFltvpMYFG-----AELVKVTPmdflRDPLLWAELISKYRGTMTAAPNFAYALlarrLRRQAKPGAFDLSSLRFAL 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 335 GGGMAVqEVVSTRWMKTTGCP-------ILEGYGLSETSPSVTLSPPTLGAFTGTI------------------------ 383
Cdd:PRK07768 283 NGAEPI-DPADVEDLLDAGARfglrpeaILPAYGMAEATLAVSFSPCGAGLVVDEVdadllaalrravpatkgntrrlat 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 384 -GLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNrPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMI 462
Cdd:PRK07768 362 lGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGYLT-MDGFIPAQDADGWLDTGDLGYLTEEGEVVVCGRVKDVI 440
|
490 500
....*....|....*....|..
gi 979216869 463 LVSGFNVYPNEIESVVAAHPGV 484
Cdd:PRK07768 441 IMAGRNIYPTDIERAAARVEGV 462
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
27-553 |
3.72e-29 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 119.97 E-value: 3.72e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 27 HLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPL 106
Cdd:cd17645 2 QLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 107 YTPRELEHQLRDSGAEAIVVlenfattlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfr 186
Cdd:cd17645 82 YPGERIAYMLADSSAKILLT------------------------------------------------------------ 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 187 talrdgarqpfqpaalNHDDVAFLQYTGGTTGVSKGATLTHRNVIaNILQndaWSQPALRKAPQIDALVtvcalplYHIL 266
Cdd:cd17645 102 ----------------NPDDLAYVIYTSGSTGLPKGVMIEHHNLV-NLCE---WHRPYFGVTPADKSLV-------YASF 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 267 ALNACML-MGARW--GAMNLLIPNPRDVDgfVKELSRY------RVNFFP-GVNTLFNALLNHpgfaaldfsGLRITLGG 336
Cdd:cd17645 155 SFDASAWeIFPHLtaGAALHVVPSERRLD--LDALNDYfnqegiTISFLPtGAAEQFMQLDNQ---------SLRVLLTG 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVvstrwmKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQ 416
Cdd:cd17645 224 GDKLKKI------ERKGYKLVNNYGPTENTVVATSFEIDKPYANIPIGKPIDNTRVYILDEALQLQPIGVAGELCIAGEG 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 417 VMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACV 490
Cdd:cd17645 298 LARGYLNRPELTAEKFIVHPFvpgermYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVL 377
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 491 GVPDSNSGEAVKLFVVRRDPAlTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17645 378 AKEDADGRKYLVAYVTAPEEI-PHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
49-554 |
6.00e-29 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 119.21 E-value: 6.00e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 49 MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAeaivvle 128
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGA------- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 nfattlqqVIARTDvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpAALNHDDVA 208
Cdd:cd05974 74 --------VYAAVD---------------------------------------------------------ENTHADDPM 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRNVIANILQNDAWSqpALRKApqiDALVTVCAlPLYhilALNACMLMGARW--GAMNLLIP 286
Cdd:cd05974 89 LLYFTSGTTSKPKLVEHTHRSYPVGHLSTMYWI--GLKPG---DVHWNISS-PGW---AKHAWSCFFAPWnaGATVFLFN 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 287 NPR-DVDGFVKELSRYRVNFFPGVNTLFNALLNHPgFAALDfSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSET 365
Cdd:cd05974 160 YARfDAKRVLAALVRYGVTTLCAPPTVWRMLIQQD-LASFD-VKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTET 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 366 SPSVTLSPPTLgAFTGTIGLPVPSTDIALLGENDEPVAQGqpgEIAI-----RGPQVMAGYWNRPDETAKVMTpDGFFKS 440
Cdd:cd05974 238 TALVGNSPGQP-VKAGSMGRPLPGYRVALLDPDGAPATEG---EVALdlgdtRPVGLMKGYAGDPDKTAHAMR-GGYYRT 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 441 GDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV-----RRDPALTTE 515
Cdd:cd05974 313 GDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVlragyEPSPETALE 392
|
490 500 510
....*....|....*....|....*....|....*....
gi 979216869 516 aLMAYCKKEFTGYKKPKYIEFRDsLPKSNIGKILRRELR 554
Cdd:cd05974 393 -IFRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELR 429
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
204-555 |
8.30e-29 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 120.51 E-value: 8.30e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAfLQYTGGTTGVSKGATLTHRNVIANILQND-AWSqpaLRKAPqidalVTVCALPLYHILALNACMLMGARwGAMN 282
Cdd:PLN03102 186 HDPIS-LNYTSGTTADPKGVVISHRGAYLSTLSAIiGWE---MGTCP-----VYLWTLPMFHCNGWTFTWGTAAR-GGTS 255
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLIpnpRDVDG--FVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRwMKTTGCPILEGY 360
Cdd:PLN03102 256 VCM---RHVTApeIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPVHVLTGGSPPPAALVKK-VQRLGFQVMHAY 331
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 361 GLSETSPSVTL-------------SPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQP-GEIAIRGPQVMAGYWNRPD 426
Cdd:PLN03102 332 GLTEATGPVLFcewqdewnrlpenQQMELKARQGVSILGLADVDVKNKETQESVPRDGKTmGEIVIKGSSIMKGYLKNPK 411
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 427 ETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVV 506
Cdd:PLN03102 412 ATSEAFK-HGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVV 490
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 507 ----------RRDPALTTEA-LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PLN03102 491 lekgettkedRVDKLVTRERdLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRD 550
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
41-554 |
8.69e-29 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 119.01 E-value: 8.69e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 41 AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSG 120
Cdd:cd17649 5 ALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYMLEDSG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 121 AeaivvlenfattlqqviartdvrHVVVASLGDLLgpvrgmavnfvarrvkklvppyrlpgatpfrtalrdgarqpfqpa 200
Cdd:cd17649 85 A-----------------------GLLLTHHPRQL--------------------------------------------- 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 201 alnhddvAFLQYTGGTTGVSKGATLTHrNVIANILQNDAwsqPALRKAPQIDAL------VTVCALPLYHILALNACMLM 274
Cdd:cd17649 97 -------AYVIYTSGSTGTPKGVAVSH-GPLAAHCQATA---ERYGLTPGDRELqfasfnFDGAHEQLLPPLICGACVVL 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 GA--RWGamnllipnprDVDGFVKELSRYRVN---FFPGVNTLFNALLNHPGfaALDFSGLRITLGGGMAVQEVVSTRWM 349
Cdd:cd17649 166 RPdeLWA----------SADELAEMVRELGVTvldLPPAYLQQLAEEADRTG--DGRPPSLRLYIFGGEALSPELLRRWL 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTtGCPILEGYGLSETSPSVTLSPPTLGAFTG----TIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRP 425
Cdd:cd17649 234 KA-PVRLFNAYGPTEATVTPLVWKCEAGAARAgasmPIGRPLGGRSAYILDADLNPVPVGVTGELYIGGEGLARGYLGRP 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 426 DETAKVMTPDGFF-------KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVpDSNSG 498
Cdd:cd17649 313 ELTAERFVPDPFGapgsrlyRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVAL-DGAGG 391
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 499 EAVKLFVVRRDP---ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd17649 392 KQLVAYVVLRAAaaqPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
19-445 |
9.10e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 120.92 E-value: 9.10e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 19 PDRYPSLAHLLDESFAT------FAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAA 92
Cdd:PRK12582 45 GPYPRSIPHLLAKWAAEapdrpwLAQREPGHGQWRKVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLA 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 93 ALRAGFTVVNVNPLYTPRELEH----QLRDSGAEAIVVLEN---FATTLQQVIArTDVRHVVVASLGDLLgpvrgmavnf 165
Cdd:PRK12582 125 AMQAGVPAAPVSPAYSLMSHDHaklkHLFDLVKPRVVFAQSgapFARALAALDL-LDVTVVHVTGPGEGI---------- 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 166 varrvkklvppyrlpGATPFR----TALRDGARQPFqpAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndaws 241
Cdd:PRK12582 194 ---------------ASIAFAdlaaTPPTAAVAAAI--AAITPDTVAKYLFTSGSTGMPKAVINTQRMMCANIAM----- 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 242 QPALR-KAPQIDALVTVCALPLYHILALNAcMLMGARWGAMNLLIPNPRDVDGFVKELSR--------YRVNFFPGVNTL 312
Cdd:PRK12582 252 QEQLRpREPDPPPPVSLDWMPWNHTMGGNA-NFNGLLWGGGTLYIDDGKPLPGMFEETIRnlreisptVYGNVPAGYAML 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 313 FNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRW----MKTTG--CPILEGYGLSETSP-SVTLSPPTLGAftGTIGL 385
Cdd:PRK12582 331 AEAMEKDDALRRSFFKNLRLMAYGGATLSDDLYERMqalaVRTTGhrIPFYTGYGATETAPtTTGTHWDTERV--GLIGL 408
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 386 PVPSTDIALLgendePVaqGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGV 445
Cdd:PRK12582 409 PLPGVELKLA-----PV--GDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAAR 461
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
46-548 |
2.71e-28 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 120.46 E-value: 2.71e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQlRGLRKGARVAVMMPNVLQYPVAVAAALRAGF------------TVVN------VNPLY 107
Cdd:PRK06814 656 NGPLTYRKLLTGAFVLGRKLK-KNTPPGENVGVMLPNANGAAVTFFALQSAGRvpaminfsagiaNILSackaaqVKTVL 734
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 108 TPRELEHQLRdsgaeaivvLENFATTLQQ---VIARTDVRHVVvaSLGDLLgpvRGMavnfvarrvkklvppyrLPGATP 184
Cdd:PRK06814 735 TSRAFIEKAR---------LGPLIEALEFgirIIYLEDVRAQI--GLADKI---KGL-----------------LAGRFP 783
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 FRTALRdgaRQPfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpALRKAPQIDALVTVCALPLYH 264
Cdd:PRK06814 784 LVYFCN---RDP--------DDPAVILFTSGSEGTPKGVVLSHRNLLANRAQ-------VAARIDFSPEDKVFNALPVFH 845
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 265 ILALNACMLMGARWGAMNLLIPNPRDVDgFVKELSrYRVN---FFpGVNTLFNALLN--HPgfaaLDFSGLRITLGGGMA 339
Cdd:PRK06814 846 SFGLTGGLVLPLLSGVKVFLYPSPLHYR-IIPELI-YDTNatiLF-GTDTFLNGYARyaHP----YDFRSLRYVFAGAEK 918
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 340 VQEvvSTR--WMKTTGCPILEGYGLSETSPSVTLSPPTLGAFtGTIGLPVPSTDIALlgendEPVA---QGqpGEIAIRG 414
Cdd:PRK06814 919 VKE--ETRqtWMEKFGIRILEGYGVTETAPVIALNTPMHNKA-GTVGRLLPGIEYRL-----EPVPgidEG--GRLFVRG 988
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 415 PQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAA-HPGVIEcACVGVP 493
Cdd:PRK06814 989 PNVMLGYLRAENPGVLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAElWPDALH-AAVSIP 1067
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 494 DSNSGEAVKLFVVRRDpaLTTEALMAYCKKEftGYKK---PKYIEFRDSLPKSNIGKI 548
Cdd:PRK06814 1068 DARKGERIILLTTASD--ATRAAFLAHAKAA--GASElmvPAEIITIDEIPLLGTGKI 1121
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
47-442 |
5.75e-28 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 117.92 E-value: 5.75e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEH----QLRDSGAE 122
Cdd:cd05921 24 RRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLMSQDLaklkHLFELLKP 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 123 AIVVLENfATTLQQVIARTDVRHV-VVASLGDLLGPvrgMAVNFVArrvkkLVppyrlpgATPFRTALrDGARQPFQPaa 201
Cdd:cd05921 104 GLVFAQD-AAPFARALAAIFPLGTpLVVSRNAVAGR---GAISFAE-----LA-------ATPPTAAV-DAAFAAVGP-- 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 202 lnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsQPALRKAPQIDALVTVCALPLYHILALNACMLMgARWGAM 281
Cdd:cd05921 165 ---DTVAKFLFTSGSTGLPKAVINTQRMLCANQAM-----LEQTYPFFGEEPPVLVDWLPWNHTFGGNHNFNL-VLYNGG 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 282 NLLI----PNPRDVDGFVKELSR----YRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRW----M 349
Cdd:cd05921 236 TLYIddgkPMPGGFEETLRNLREisptVYFNVPAGWEMLVAALEKDEALRRRFFKRLKLMFYAGAGLSQDVWDRLqalaV 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 350 KTTGCPI--LEGYGLSETSPSVTLSPpTLGAFTGTIGLPVPSTDIALlgendepVAQGQPGEIAIRGPQVMAGYWNRPDE 427
Cdd:cd05921 316 ATVGERIpmMAGLGATETAPTATFTH-WPTERSGLIGLPAPGTELKL-------VPSGGKYEVRVKGPNVTPGYWRQPEL 387
|
410
....*....|....*
gi 979216869 428 TAKVMTPDGFFKSGD 442
Cdd:cd05921 388 TAQAFDEEGFYCLGD 402
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
21-553 |
1.31e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 118.91 E-value: 1.31e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 21 RYPSLAH---LLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAG 97
Cdd:PRK12316 3052 EYPLERGvhrLFEEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAG 3131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 98 FTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLQQVIARTDVRhvvvaslgdllgpvrgmavnfvarrvkklvppy 177
Cdd:PRK12316 3132 GAYVPLDPEYPEERLAYMLEDSGAQLLLSQSHLRLPLAQGVQVLDLD--------------------------------- 3178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 178 rlpgatpfrtALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHrnviANILQNDAWSQPALRKAPQIDAL--- 254
Cdd:PRK12316 3179 ----------RGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRH----SALSNHLCWMQQAYGLGVGDRVLqft 3244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 255 ---VTVCALPLYHILALNACMLMGARWGAmnllipnpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGfaALDFSGLR 331
Cdd:PRK12316 3245 tfsFDVFVEELFWPLMSGARVVLAGPEDW--------RDPALLVELINSEGVDVLHAYPSMLQAFLEEED--AHRCTSLK 3314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 332 ITLGGGMAVQEVVSTRWMktTGCPILEGYGLSETSPSVT-LSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEI 410
Cdd:PRK12316 3315 RIVCGGEALPADLQQQVF--AGLPLYNLYGPTEATITVThWQCVEEGKDAVPIGRPIANRACYILDGSLEPVPVGALGEL 3392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 411 AIRGPQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGV 484
Cdd:PRK12316 3393 YLGGEGLARGYHNRPGLTAERFVPDPFvpgerlYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWV 3472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 485 IECACVGVpdsnSGEAVKLFVVRRDPALTT-EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK12316 3473 REAVVLAV----DGRQLVAYVVPEDEAGDLrEALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKAL 3538
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
33-505 |
2.81e-27 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 115.87 E-value: 2.81e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 33 FATFAARNAYACMGRT--------------MTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGF 98
Cdd:PRK09192 20 FPTLVEALDYAALGEAgmnfydrrgqleeaLPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 99 TVVnvnPLYTP-----RE-----LEHQLRDSGAEAIVVLENFATTLQQVIARTDVRHVVVASLGDLLgPVRGMAvnfvar 168
Cdd:PRK09192 100 VPV---PLPLPmgfggREsyiaqLRGMLASAQPAAIITPDELLPWVNEATHGNPLLHVLSHAWFKAL-PEADVA------ 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 169 rvkklvppyrLPGATPfrtalrdgarqpfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQPALRKA 248
Cdd:PRK09192 170 ----------LPRPTP--------------------DDIAYLQYSSGSTRFPRGVIITHRALMANL---RAISHDGLKVR 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 249 PQIDAlvtVCALPLYHILALNACML-----------MGARWGAMNLLIpnprdvdgFVKELSRYR--VNFFPGVNTLFNA 315
Cdd:PRK09192 217 PGDRC---VSWLPFYHDMGLVGFLLtpvatqlsvdyLPTRDFARRPLQ--------WLDLISRNRgtISYSPPFGYELCA 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 316 L-LNHPGFAALDFSGLRIT-LGGGM-------AVQEVVSTRWMKTTGcpILEGYGLSETSPSVTLSPPTLGAFTGTI--- 383
Cdd:PRK09192 286 RrVNSKDLAELDLSCWRVAgIGADMirpdvlhQFAEAFAPAGFDDKA--FMPSYGLAEATLAVSFSPLGSGIVVEEVdrd 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 384 ------------------------GLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRpDETAKVMTPDGFFK 439
Cdd:PRK09192 364 rleyqgkavapgaetrrvrtfvncGKALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSGYFRD-EESQDVLAADGWLD 442
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 440 SGDVGVMDEhGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVI--ECACVGVPDSNsGEAVKLFV 505
Cdd:PRK09192 443 TGDLGYLLD-GYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELRsgDAAAFSIAQEN-GEKIVLLV 508
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
47-555 |
3.42e-27 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 116.01 E-value: 3.42e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAG--FTVVNVNplYTPRELEHQLRDSGAEAI 124
Cdd:PRK00174 97 RKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGavHSVVFGG--FSAEALADRIIDAGAKLV 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 VvlenfaTTLQQV---------------IARTD-VRHVVVaslgdllgpVR--GMAVNFVARR------VKKLVPPYrlp 180
Cdd:PRK00174 175 I------TADEGVrggkpiplkanvdeaLANCPsVEKVIV---------VRrtGGDVDWVEGRdlwwheLVAGASDE--- 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 181 gatpfrtalrdgarqpFQPAALNHDDVAFLQYTGGTTGVSKG-----------ATLTHRNVIaNILQND--------AWs 241
Cdd:PRK00174 237 ----------------CEPEPMDAEDPLFILYTSGSTGKPKGvlhttggylvyAAMTMKYVF-DYKDGDvywctadvGW- 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 242 qpalrkapqidalVTVCALPLYHILALNACMLM--GArwgamnlliPNPRDVDGFVKELSRYRVNFFPGVNTLFNALL-- 317
Cdd:PRK00174 299 -------------VTGHSYIVYGPLANGATTLMfeGV---------PNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMke 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 318 --NHPgfAALDFSGLRI------------------TLGGGMAVqeVVSTRWMKTTGC----PIlegyglsetsPSVTLSP 373
Cdd:PRK00174 357 gdEHP--KKYDLSSLRLlgsvgepinpeawewyykVVGGERCP--IVDTWWQTETGGimitPL----------PGATPLK 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 374 PtlgaftGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG--PQVMAGYWNRPDETAKV---MTPDGFFkSGDVGVMDE 448
Cdd:PRK00174 423 P------GSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDpwPGMMRTIYGDHERFVKTyfsTFKGMYF-TGDGARRDE 495
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 449 HGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALM----AYCKKE 524
Cdd:PRK00174 496 DGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSDELRkelrNWVRKE 575
|
570 580 590
....*....|....*....|....*....|.
gi 979216869 525 FTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK00174 576 IGPIAKPDVIQFAPGLPKTRSGKIMRRILRK 606
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
48-518 |
4.65e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 115.42 E-value: 4.65e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGlRKGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYTPRELEHQLRDSGA----EA 123
Cdd:PRK05850 35 TLTWSQLYRRTLNVAEELRRHG-STGDRAVILAPQGLEYIVAFLGALQAGLIAV---PLSVPQGGAHDERVSAVlrdtSP 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 124 IVVLenfaTTLQQViarTDVRHVVVASLGDLLGPVrgMAVNfvarrvkklvppyrlpgatpfrtALRDGARQPFQPAALN 203
Cdd:PRK05850 111 SVVL----TTSAVV---DDVTEYVAPQPGQSAPPV--IEVD-----------------------LLDLDSPRGSDARPRD 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAFLQYTGGTTGVSKGATLTHRNVIANILQ--NDAWSQPAlrKAPQIDaLVTVCALPLYH----ILALNACMLMG-- 275
Cdd:PRK05850 159 LPSTAYLQYTSGSTRTPAGVMVSHRNVIANFEQlmSDYFGDTG--GVPPPD-TTVVSWLPFYHdmglVLGVCAPILGGcp 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 276 -------------ARWgaMNLLIPNPRDVDG---FVKELSRYRVNffpgvntlfNALLnhpgfAALDFSGLRITLGGGMA 339
Cdd:PRK05850 236 avltspvaflqrpARW--MQLLASNPHAFSAapnFAFELAVRKTS---------DDDM-----AGLDLGGVLGIISGSER 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 340 VQEVVSTRWMKTtgcpiLEGYGLSETS--PSVTLSPPTLGAFTGTIGLPVPSTDI-------------------ALL--G 396
Cdd:PRK05850 300 VHPATLKRFADR-----FAPFNLRETAirPSYGLAEATVYVATREPGQPPESVRFdyeklsaghakrcetgggtPLVsyG 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 397 ENDEPV------------AQGQPGEIAIRGPQVMAGYWNRPDETAKVM----------TPDG-FFKSGDVGVMDEHGFVr 453
Cdd:PRK05850 375 SPRSPTvrivdpdtciecPAGTVGEIWVHGDNVAAGYWQKPEETERTFgatlvdpspgTPEGpWLRTGDLGFISEGELF- 453
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 454 IVDRKKDMILVSGFNVYPNEIESVVAAHPGViECACVGVPDSNSGEAVKLFVVRRDPALTTEALM 518
Cdd:PRK05850 454 IVGRIKDLLIVDGRNHYPDDIEATIQEITGG-RVAAISVPDDGTEKLVAIIELKKRGDSDEEAMD 517
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
205-547 |
3.23e-26 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 110.16 E-value: 3.23e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVaFLQYTGGTTGVSKGATLTHR-------NVIANILQNDAWSQPALRKAPQIDALVTVCALPLYHILALNACMlmGAR 277
Cdd:cd05924 4 DDL-YILYTGGTTGMPKGVMWRQEdifrmlmGGADFGTGEFTPSEDAHKAAAAAAGTVMFPAPPLMHGTGSWTAF--GGL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 278 WGAMNLLIPNPR-DVDGFVKELSRYRVNFFPGVNT-----LFNALlnhPGFAALDFSGLRITLGGGMAVQEVVSTRWMKT 351
Cdd:cd05924 81 LGGQTVVLPDDRfDPEEVWRTIEKHKVTSMTIVGDamarpLIDAL---RDAGPYDLSSLFAISSGGALLSPEVKQGLLEL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 352 TgcP---ILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGpQVMAGYWNRPDET 428
Cdd:cd05924 158 V--PnitLVDAFGSSETGFTGSGHSAGSGPETGPFTRANPDTVVLDDDGRVVPPGSGGVGWIARRG-HIPLGYYGDEAKT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 429 AKVM-TPDG--FFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFV 505
Cdd:cd05924 235 AETFpEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVV 314
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 979216869 506 VRRDPA-LTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:cd05924 315 QLREGAgVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
195-554 |
7.31e-26 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 111.37 E-value: 7.31e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 195 QPFQPaaLNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPqidalVTVCALPLYHILALNACMLM 274
Cdd:cd05915 145 ADPVR--VPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVDGTALSEKD-----VVLPVVPMFHVNAWCLPYAA 217
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 GARWGAMNLLIPNPRDvDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVsTRWMKTTGC 354
Cdd:cd05915 218 TLVGAKQVLPGPRLDP-ASLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPRSL-IARFERMGV 295
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 PILEGYGLSETspsvtlspptLGAFTGTIGLP----VPSTDIALLGEND------------EPVAQGQPGE------IAI 412
Cdd:cd05915 296 EVRQGYGLTET----------SPVVVQNFVKShlesLSEEEKLTLKAKTglpiplvrlrvaDEEGRPVPKDgkalgeVQL 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 413 RGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGV 492
Cdd:cd05915 366 KGPWITGGYYGNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAI 445
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 493 PDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKK-PKYIEFRDSLPKSNIGKILRRELR 554
Cdd:cd05915 446 PHPKWQERPLAVVVPRGEKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALR 508
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
23-561 |
1.45e-25 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 112.18 E-value: 1.45e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 23 PSLAHLLDESFATFAARN----AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGF 98
Cdd:PRK12467 3091 YPSERLVHQLIEAQVARTpeapALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGG 3170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 99 TVVNVNPLYtPRE-LEHQLRDSGAEaivVLENFATTLQQVIARTDVrHVVVASLGDLLGpvrgmavnfvarrvkklvppy 177
Cdd:PRK12467 3171 AYVPLDPEY-PRErLAYMIEDSGVK---LLLTQAHLLEQLPAPAGD-TALTLDRLDLNG--------------------- 3224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 178 rLPGATPfrTALRDGarqpfqpaalnhDDVAFLQYTGGTTGVSKGATLTHrNVIANILQndawsqpALRKAPQIDALVTV 257
Cdd:PRK12467 3225 -YSENNP--STRVMG------------ENLAYVIYTSGSTGKPKGVGVRH-GALANHLC-------WIAEAYELDANDRV 3281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 258 calplyhiLALNACMLMGARW--------GAMNLLIPNP-RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAalDFS 328
Cdd:PRK12467 3282 --------LLFMSFSFDGAQErflwtlicGGCLVVRDNDlWDPEELWQAIHAHRISIACFPPAYLQQFAEDAGGA--DCA 3351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 329 GLRITLGGGMAV----QEVVStRWMKTTGcpILEGYGLSETSPSVTL----SPPTLGAFTGTIGLPVPSTDIALLGENDE 400
Cdd:PRK12467 3352 SLDIYVFGGEAVppaaFEQVK-RKLKPRG--LTNGYGPTEAVVTVTLwkcgGDAVCEAPYAPIGRPVAGRSIYVLDGQLN 3428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 401 PVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNE 473
Cdd:PRK12467 3429 PVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFsgsggrlYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGE 3508
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 474 IESVVAAHPGVIECACVGVpDSNSGEAVKLFVVRRDP-ALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRE 552
Cdd:PRK12467 3509 IEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVPADPqGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKA 3587
|
....*....
gi 979216869 553 LRDEHGQTA 561
Cdd:PRK12467 3588 LPDPDAKGS 3596
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
13-553 |
4.28e-25 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 110.52 E-value: 4.28e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 13 VPATvdpdrypSLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAA 92
Cdd:PRK10252 455 IPET-------TLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHA 527
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 93 ALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVLENFATTLqqviartdvrhvvvASLGDLLgpvrgmavnfvarrvkk 172
Cdd:PRK10252 528 IVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTADQLPRF--------------ADVPDLT----------------- 576
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 173 lVPPYRLPGATPFRTALRdgarqPFQPaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILqndaWSQP--------- 243
Cdd:PRK10252 577 -SLCYNAPLAPQGAAPLQ-----LSQP-----HHTAYIIFTSGSTGRPKGVMVGQTAIVNRLL----WMQNhypltaddv 641
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 244 ALRKAP-QIDALVTVCALPlyhilalnacMLMGARwgamnLLIPNP---RDVDGFVKELSRYRV---NFFPGVNTLFNAL 316
Cdd:PRK10252 642 VLQKTPcSFDVSVWEFFWP----------FIAGAK-----LVMAEPeahRDPLAMQQFFAEYGVtttHFVPSMLAAFVAS 706
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 317 LNhPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTL---SPPTLGAFTGT---IGLPVPST 390
Cdd:PRK10252 707 LT-PEGARQSCASLRQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWypaFGEELAAVRGSsvpIGYPVWNT 785
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 391 DIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILV 464
Cdd:PRK10252 786 GLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFapgermYRTGDVARWLDDGAVEYLGRSDDQLKI 865
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 465 SGFNVYPNEIESVVAAHPGV---IECACV-GVPDSNSGEAVKL---FVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFR 537
Cdd:PRK10252 866 RGQRIELGEIDRAMQALPDVeqaVTHACViNQAAATGGDARQLvgyLVSQSGLPLDTSALQAQLRERLPPHMVPVVLLQL 945
|
570
....*....|....*.
gi 979216869 538 DSLPKSNIGKILRREL 553
Cdd:PRK10252 946 DQLPLSANGKLDRKAL 961
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
47-517 |
5.07e-25 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 109.43 E-value: 5.07e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGlRKGARVAVMMPNVLQYPVAVAAALRAGFTVVnvnPLYTPRELEHqlrdsgaeaivv 126
Cdd:PRK07769 54 RDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLIAFFGALYAGRIAV---PLFDPAEPGH------------ 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlqqviarTDVRHVVvasLGDLlGPVRGMAVNFVARRVKKLVPPyrLPGATPFRT----ALRDGARQPFQPAAL 202
Cdd:PRK07769 118 --------------VGRLHAV---LDDC-TPSAILTTTDSAEGVRKFFRA--RPAKERPRViavdAVPDEVGATWVPPEA 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpalrkapQIDAL------VTVCALPLYHILALNACMLMGA 276
Cdd:PRK07769 178 NEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQ-------------VIDALegqegdRGVSWLPFFHDMGLITVLLPAL 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 277 RWGAMNLLIPNP--RDVDGFVKELSRYRvnffPGVNTLFNALlnhPGFA---------------ALDFSGLRITLGGgma 339
Cdd:PRK07769 245 LGHYITFMSPAAfvRRPGRWIRELARKP----GGTGGTFSAA---PNFAfehaaarglpkdgepPLDLSNVKGLLNG--- 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 340 vQEVVSTRWMKTTgCPILEGYGLSETS--PSVTLSPPTLgaFTGTIG--------------------LPVPSTD------ 391
Cdd:PRK07769 315 -SEPVSPASMRKF-NEAFAPYGLPPTAikPSYGMAEATL--FVSTTPmdeeptviyvdrdelnagrfVEVPADApnavaq 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 392 ------------IALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVM---------------TPDG--FFKSGD 442
Cdd:PRK07769 391 vsagkvgvsewaVIVDPETASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqnilksrlseshaegAPDDalWVRTGD 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 443 VGVMDEhGFVRIVDRKKDMILVSGFNVYPNEIESVV-----AAHPGVIecACVGVP----------DSNSGeavklfvVR 507
Cdd:PRK07769 471 YGVYFD-GELYITGRVKDLVIIDGRNHYPQDLEYTAqeatkALRTGYV--AAFSVPanqlpqvvfdDSHAG-------LK 540
|
570
....*....|
gi 979216869 508 RDPALTTEAL 517
Cdd:PRK07769 541 FDPEDTSEQL 550
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
193-547 |
9.48e-25 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 108.64 E-value: 9.48e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 193 ARQPFQPaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRkapqiDALVTvcALPLYHILALNACM 272
Cdd:PRK08043 358 AQVKQQP-----EDAALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPN-----DRFMS--ALPLFHSFGLTVGL 425
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 273 LMGARWGAMNLLIPNP---RdvdgFVKELSRYR---VNFfpGVNTLfnaLLNHPGFA-ALDFSGLRITLGGGMAVQEVVS 345
Cdd:PRK08043 426 FTPLLTGAEVFLYPSPlhyR----IVPELVYDRnctVLF--GTSTF---LGNYARFAnPYDFARLRYVVAGAEKLQESTK 496
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 346 TRWMKTTGCPILEGYGLSETSPSVTLSPPtLGAFTGTIGLPVPSTDIALLgendePVA---QGqpGEIAIRGPQVMAGYW 422
Cdd:PRK08043 497 QLWQDKFGLRILEGYGVTECAPVVSINVP-MAAKPGTVGRILPGMDARLL-----SVPgieQG--GRLQLKGPNIMNGYL 568
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 423 --NRPD-------ETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVP 493
Cdd:PRK08043 569 rvEKPGvlevptaENARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKS 648
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 494 DSNSGEAVKLFVVrrDPALTTEALMAYCKKefTGYKK---PKYIEFRDSLPKSNIGK 547
Cdd:PRK08043 649 DASKGEALVLFTT--DSELTREKLQQYARE--HGVPElavPRDIRYLKQLPLLGSGK 701
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
50-484 |
2.66e-24 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 107.21 E-value: 2.66e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAAlrAGFTVVNVnPLYtprelehqlrDS-GAEAIvvle 128
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEAC--AAHSLICV-PLY----------DTlGPGAV---- 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 129 NFattlqqVI--ARTDVRHVVVASLGDLLGPVRGMAvnfvaRRVKKLVPPYRLPGATPFRTA------------LRDGAR 194
Cdd:PLN02430 141 DY------IVdhAEIDFVFVQDKKIKELLEPDCKSA-----KRLKAIVSFTSVTEEESDKASqigvktyswidfLHMGKE 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 195 QPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPQIDalVTVCALPLYHILA------- 267
Cdd:PLN02430 210 NPSETNPPKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVDLFMEQFEDKMTHDD--VYLSFLPLAHILDrmieeyf 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 268 LNACMLMGARWGAMN------------LLIPNPRDV----DGFVKELSRYRvnffPGVNTLFNALLNH------------ 319
Cdd:PLN02430 288 FRKGASVGYYHGDLNalrddlmelkptLLAGVPRVFerihEGIQKALQELN----PRRRLIFNALYKYklawmnrgyshk 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 ---PGFAALDFSGLRITLGG-------GMAVQEVVSTRWMKTTGCP-ILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVP 388
Cdd:PLN02430 364 kasPMADFLAFRKVKAKLGGrlrllisGGAPLSTEIEEFLRVTSCAfVVQGYGLTETLGPTTLGFPDEMCMLGTVGAPAV 443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 389 STDIAL-----LGENdePVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMIL 463
Cdd:PLN02430 444 YNELRLeevpeMGYD--PLGEPPRGEICVRGKCLFSGYYKNPELTEEVMK-DGWFHTGDIGEILPNGVLKIIDRKKNLIK 520
|
490 500
....*....|....*....|..
gi 979216869 464 VS-GFNVYPNEIESVVAAHPGV 484
Cdd:PLN02430 521 LSqGEYVALEYLENVYGQNPIV 542
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
24-557 |
3.64e-24 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 106.75 E-value: 3.64e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 24 SLAHLLDESFATFAARNAYACM-------GRT--MTYAELDTYSRHLGAWLQlRGLRKGARVAVMMPNVLQYPVAVAAAL 94
Cdd:PRK12476 35 TLISLIERNIANVGDTVAYRYLdhshsaaGCAveLTWTQLGVRLRAVGARLQ-QVAGPGDRVAILAPQGIDYVAGFFAAI 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 95 RAGFTVVnvnPLYTPR------ELEHQLRDsgAEAIVVLENFATTlqqviartdvrhvvvaslgdllGPVRGmavnFVAR 168
Cdd:PRK12476 114 KAGTIAV---PLFAPElpghaeRLDTALRD--AEPTVVLTTTAAA----------------------EAVEG----FLRN 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 169 RVKKLVPPYRLPGATPfrtalrDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANILQndawsqpalrKA 248
Cdd:PRK12476 163 LPRLRRPRVIAIDAIP------DSAGESFVPVELDTDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQ----------MI 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 249 PQIDAL----VTVCALPLYHILALNACMLMGARWGAMNLLIPNP--RDVDGFVKEL---SRYRVNFFPGVNTLFnALLNH 319
Cdd:PRK12476 227 LSIDLLdrntHGVSWLPLYHDMGLSMIGFPAVYGGHSTLMSPTAfvRRPQRWIKALsegSRTGRVVTAAPNFAY-EWAAQ 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 320 PGF----AALDFSGLRITLGggmavQEVVSTRWMKTTGCPILEgYGLSETS--PSVTLSPPTLgaFTGTIG----LPVPS 389
Cdd:PRK12476 306 RGLpaegDDIDLSNVVLIIG-----SEPVSIDAVTTFNKAFAP-YGLPRTAfkPSYGIAEATL--FVATIApdaePSVVY 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 390 TDIALLG---------ENDEPVAQ-------------------------GQPGEIAIRGPQVMAGYWNRPDETAKVM--- 432
Cdd:PRK12476 378 LDREQLGagravrvaaDAPNAVAHvscgqvarsqwavivdpdtgaelpdGEVGEIWLHGDNIGRGYWGRPEETERTFgak 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 433 -------------TPDG--FFKSGDVGV-MDehGFVRIVDRKKDMILVSGFNVYPNEIESVVA-AHPGVIE--CACVGVP 493
Cdd:PRK12476 458 lqsrlaegshadgAADDgtWLRTGDLGVyLD--GELYITGRIADLIVIDGRNHYPQDIEATVAeASPMVRRgyVTAFTVP 535
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 494 DSNSGEAVklfVV--------RRDPALTTEALMAYCKKEFTgykkpkyIEFRD-------SLPKSNIGKILRRELRDEH 557
Cdd:PRK12476 536 AEDNERLV---IVaeraagtsRADPAPAIDAIRAAVSRRHG-------LAVADvrlvpagAIPRTTSGKLARRACRAQY 604
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
47-556 |
1.13e-23 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 104.08 E-value: 1.13e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPlytprelehqlrdsgaeaivv 126
Cdd:cd05910 1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDP--------------------- 59
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlqqviaRTDVRHVVvaslgdllgpvrgmavnfvarrvkklvppyrlpgatpfrTALRDGARQPF--QPAAlnh 204
Cdd:cd05910 60 -------------GMGRKNLK---------------------------------------QCLQEAEPDAFigIPKA--- 84
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqPALRKAPQIDA-LVTVCALPLYHILalNACMLMGARWGAMNL 283
Cdd:cd05910 85 DEPAAILFTSGSTGTPKGVVYRHGTFAAQI--------DALRQLYGIRPgEVDLATFPLFALF--GPALGLTSVIPDMDP 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIP---NPRDVDGFVKElsrYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVV--STRWMKTTGCPILE 358
Cdd:cd05910 155 TRParaDPQKLVGAIRQ---YGVSIVFGSPALLERVARYCAQHGITLPSLRRVLSAGAPVPIALaaRLRKMLSDEAEILT 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 359 GYGLSETSP----------SVTLSPPTLGAFTgTIGLPVPSTDIALLGENDEPVAQ---------GQPGEIAIRGPQVMA 419
Cdd:cd05910 232 PYGATEALPvssigsrellATTTAATSGGAGT-CVGRPIPGVRVRIIEIDDEPIAEwddtlelprGEIGEITVTGPTVTP 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 GYWNRPDETA--KVMTPDGFF--KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVpdS 495
Cdd:cd05910 311 TYVNRPVATAlaKIDDNSEGFwhRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALVGV--G 388
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 496 NSGEAVKLFVVRRDPALTT------EALMAYCKKEFTGYKKPKYIeFRDSLP---KSNiGKILRRELRDE 556
Cdd:cd05910 389 KPGCQLPVLCVEPLPGTITprarleQELRALAKDYPHTQRIGRFL-IHPSFPvdiRHN-AKIFREKLAVW 456
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
174-554 |
1.73e-23 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 102.05 E-value: 1.73e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 174 VPPYRLPGATPFRTALRDGarQPFQpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANilqNDAWSQpALRKAPQida 253
Cdd:PRK07824 12 VPAQDERRAALLRDALRVG--EPID------DDVALVVATSGTTGTPKGAMLTAAALTAS---ADATHD-RLGGPGQ--- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 254 lvTVCALPLYHILALNACM---LMGARwgAMNLLIP---NPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAAL-D 326
Cdd:PRK07824 77 --WLLALPAHHIAGLQVLVrsvIAGSE--PVELDVSagfDPTALPRAVAELGGGRRYTSLVPMQLAKALDDPAATAALaE 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 FSGlrITLGGGMAVQEVVstRWMKTTGCPILEGYGLSETSpsvtlspptlgafTGTI--GLPVPSTDIALLGendepvaq 404
Cdd:PRK07824 153 LDA--VLVGGGPAPAPVL--DAAAAAGINVVRTYGMSETS-------------GGCVydGVPLDGVRVRVED-------- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 405 gqpGEIAIRGPQVMAGYWNRPDETAkvMTPDGFFKSGDVGVMDEhGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGV 484
Cdd:PRK07824 208 ---GRIALGGPTLAKGYRNPVDPDP--FAEPGWFRTDDLGALDD-GVLTVLGRADDAISTGGLTVLPQVVEAALATHPAV 281
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 979216869 485 IECACVGVPDSNSGEAVKLFVVRR-DPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK07824 282 ADCAVFGLPDDRLGQRVVAAVVGDgGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALV 352
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
47-459 |
2.42e-23 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 104.29 E-value: 2.42e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:PTZ00216 120 RYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVC 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 LENFATTLQQVIARTDVRHVVVASLGDLLGPVRGMAVNFVArrvkklvppyrlpgatpFRTALRDGARQPFQ---PAALN 203
Cdd:PTZ00216 200 NGKNVPNLLRLMKSGGMPNTTIIYLDSLPASVDTEGCRLVA-----------------WTDVVAKGHSAGSHhplNIPEN 262
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 204 HDDVAFLQYTGGTTGVSKGATLTHRNVIANILqndawsqpALrkAPQIDALV-------TVCA-LPLYHILALNACMLMG 275
Cdd:PTZ00216 263 NDDLALIMYTSGTTGDPKGVMHTHGSLTAGIL--------AL--EDRLNDLIgppeedeTYCSyLPLAHIMEFGVTNIFL 332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 276 ARwGAmnlLI--PNPRDV-DGFVK---ELSRYRVNFFPGVNTLFN-------ALLNHPG--------------FAALD-- 326
Cdd:PTZ00216 333 AR-GA---LIgfGSPRTLtDTFARphgDLTEFRPVFLIGVPRIFDtikkaveAKLPPVGslkrrvfdhayqsrLRALKeg 408
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 ----------FSGLRITLGGGM------------AVQEvvstrWMKTTGCPILEGYGLSETSpsvtlsppTLGA--FTG- 381
Cdd:PTZ00216 409 kdtpywnekvFSAPRAVLGGRVramlsgggplsaATQE-----FVNVVFGMVIQGWGLTETV--------CCGGiqRTGd 475
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 382 ----TIGLPVPSTDIALLG-----ENDEPvaqgQP-GEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGF 451
Cdd:PTZ00216 476 lepnAVGQLLKGVEMKLLDteeykHTDTP----EPrGEILLRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIAANGT 551
|
....*...
gi 979216869 452 VRIVDRKK 459
Cdd:PTZ00216 552 LRIIGRVK 559
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
25-553 |
4.91e-23 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 104.48 E-value: 4.91e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 25 LAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:PRK05691 1133 LPELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLD 1212
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGAEAIvvlenfattLQQViartdvrhvvvASLGDLLGPvrgmavnfvarrvkklvppyrlPGATP 184
Cdd:PRK05691 1213 PDYPAERLAYMLADSGVELL---------LTQS-----------HLLERLPQA----------------------EGVSA 1250
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 FRTALRDGARQPFQPAALN-HDD-VAFLQYTGGTTGVSKGATLTHRnVIANILQndaWSQPA---------LRKAP-QID 252
Cdd:PRK05691 1251 IALDSLHLDSWPSQAPGLHlHGDnLAYVIYTSGSTGQPKGVGNTHA-ALAERLQ---WMQATyalddsdvlMQKAPiSFD 1326
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 253 ALVTVCALPLyhilalnacmLMGARwgamnLLIPNP---RDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALdfSG 329
Cdd:PRK05691 1327 VSVWECFWPL----------ITGCR-----LVLAGPgehRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAAC--TS 1389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 330 LRITLGGGMAVQEVVSTRWMKT-TGCPILEGYGLSETSPSVT----------LSPptlgaftgtIGLPVPSTDIALLGEN 398
Cdd:PRK05691 1390 LRRLFSGGEALPAELRNRVLQRlPQVQLHNRYGPTETAINVThwqcqaedgeRSP---------IGRPLGNVLCRVLDAE 1460
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 399 DEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYP 471
Cdd:PRK05691 1461 LNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPLgedgarlYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEP 1540
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 472 NEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRR 551
Cdd:PRK05691 1541 EEIQARLLAQPGVAQAAVLVREGAAGAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRR 1620
|
..
gi 979216869 552 EL 553
Cdd:PRK05691 1621 AL 1622
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
29-553 |
6.75e-23 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 104.09 E-value: 6.75e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 29 LDESFATFAARNAYA----CMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVN 104
Cdd:PRK05691 2190 LHGLFAAQAARTPQApaltFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLD 2269
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 105 PLYTPRELEHQLRDSGaeaIVVLENFAttlqqviartdvrhVVVASLGDLlgPVRgmavnfVARrvkklvppYRLPGATP 184
Cdd:PRK05691 2270 PEYPLERLHYMIEDSG---IGLLLSDR--------------ALFEALGEL--PAG------VAR--------WCLEDDAA 2316
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 185 FRTALRDGArqpfqPAALNH-DDVAFLQYTGGTTGVSKGATLTHRNVianilqndawsqpALRKAPQIDAL---VTVCAL 260
Cdd:PRK05691 2317 ALAAYSDAP-----LPFLSLpQHQAYLIYTSGSTGKPKGVVVSHGEI-------------AMHCQAVIERFgmrADDCEL 2378
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 261 PLYHI------LALNACMLMGAR--------WGAmnllipnpRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGfaalD 326
Cdd:PRK05691 2379 HFYSInfdaasERLLVPLLCGARvvlraqgqWGA--------EEICQLIREQQVSILGFTPSYGSQLAQWLAGQG----E 2446
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 FSGLRITLGGGMAVQEVVSTRwMKTTGCP--ILEGYGLSET--SPSVTLSPPTL--GAFTGTIGLPVPSTDIALLGENDE 400
Cdd:PRK05691 2447 QLPVRMCITGGEALTGEHLQR-IRQAFAPqlFFNAYGPTETvvMPLACLAPEQLeeGAASVPIGRVVGARVAYILDADLA 2525
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 401 PVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNE 473
Cdd:PRK05691 2526 LVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFaadggrlYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGE 2605
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 474 IESVVAAHPGVIECACVGVpDSNSGEAVKLFVVRRDPALTT-------EALMAYCKKEFTGYKKPKYIEFRDSLPKSNIG 546
Cdd:PRK05691 2606 IESRLLEHPAVREAVVLAL-DTPSGKQLAGYLVSAVAGQDDeaqaalrEALKAHLKQQLPDYMVPAHLILLDSLPLTANG 2684
|
....*..
gi 979216869 547 KILRREL 553
Cdd:PRK05691 2685 KLDRRAL 2691
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
203-553 |
1.18e-22 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 100.97 E-value: 1.18e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 203 NHDDVAFLQYTGGTTGVSKGATLTHRNVIanilqNDAWSQPALRKAPQIDALVT-------VCALPLYHILALNACMLMg 275
Cdd:cd17644 104 QPENLAYVIYTSGSTGKPKGVMIEHQSLV-----NLSHGLIKEYGITSSDRVLQfasiafdVAAEEIYVTLLSGATLVL- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 276 arwgAMNLLIPNPRDVDGFVKELSRYRVNFFPGV-NTLFNALLnhPGFAALDfSGLRITLGGGMAVQEVVSTRWMKTTGC 354
Cdd:cd17644 178 ----RPEEMRSSLEDFVQYIQQWQLTVLSLPPAYwHLLVLELL--LSTIDLP-SSLRLVIVGGEAVQPELVRQWQKNVGN 250
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 PI--LEGYGLSETSPSVTLSPPTLGAFTG----TIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDET 428
Cdd:cd17644 251 FIqlINVYGPTEATIAATVCRLTQLTERNitsvPIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELT 330
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 429 AKVMTPDGF--------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGViECACVGVPDSNSGEA 500
Cdd:cd17644 331 AEKFISHPFnsseserlYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDV-KTAVVIVREDQPGNK 409
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 501 --VKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17644 410 rlVAYIVPHYEESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
46-478 |
1.40e-22 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 102.94 E-value: 1.40e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGlRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRElEHQLRdsgaeaiv 125
Cdd:PRK05691 38 GVVLSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAYPPESARR-HHQER-------- 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 vlenfattLQQVIARTDVRHVVVASlgDLLGPVRGMAvNFVARRVKKLVPPYRLPgatpfrTALRDGARQPfqpaALNHD 205
Cdd:PRK05691 108 --------LLSIIADAEPRLLLTVA--DLRDSLLQME-ELAAANAPELLCVDTLD------PALAEAWQEP----ALQPD 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIANilqndawsQPALRKAPQIDAL---VTVCALPLYHILALNACMLMGARWGAMN 282
Cdd:PRK05691 167 DIAFLQYTSGSTALPKGVQVSHGNLVAN--------EQLIRHGFGIDLNpddVIVSWLPLYHDMGLIGGLLQPIFSGVPC 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 283 LLI-PN---PRDVDgFVKELSRYRVNFFPGVN---TLFNALLNHPGFAALDFSGLRITLGGGMAV-QEVVSTRWMKTTGC 354
Cdd:PRK05691 239 VLMsPAyflERPLR-WLEAISEYGGTISGGPDfayRLCSERVSESALERLDLSRWRVAYSGSEPIrQDSLERFAEKFAAC 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 355 -----PILEGYGLSETSPSVTLSP---------------------PTLGAFTGTIGLPVPSTDIALLGEND-EPVAQGQP 407
Cdd:PRK05691 318 gfdpdSFFASYGLAEATLFVSGGRrgqgipaleldaealarnraePGTGSVLMSCGRSQPGHAVLIVDPQSlEVLGDNRV 397
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 979216869 408 GEIAIRGPQVMAGYWNRPDETAKVMTP-DG--FFKSGDVGVMdEHGFVRIVDRKKDMILVSGFNVYPNEIESVV 478
Cdd:PRK05691 398 GEIWASGPSIAHGYWRNPEASAKTFVEhDGrtWLRTGDLGFL-RDGELFVTGRLKDMLIVRGHNLYPQDIEKTV 470
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
210-553 |
1.57e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 100.11 E-value: 1.57e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 210 LQYTGGTTGVSKGATLTHRNVIANIlqnDAWSQpALRKApqiDALVTVCALPLYHILALNACMLMGARWGAMNLLI--PN 287
Cdd:PRK08308 106 LQYSSGTTGEPKLIRRSWTEIDREI---EAYNE-ALNCE---QDETPIVACPVTHSYGLICGVLAALTRGSKPVIItnKN 178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 288 PRDVdgfVKELSRYRVNFFPGVNTLFNALLNHP----GFAALDFSGLRITLGGGMAVQEvVSTRWMKTTGCpilegyglS 363
Cdd:PRK08308 179 PKFA---LNILRNTPQHILYAVPLMLHILGRLLpgtfQFHAVMTSGTPLPEAWFYKLRE-RTTYMMQQYGC--------S 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 364 EtSPSVTLSPPTlgAFTGTIGLPVPSTDIAlLGENdepvaQGQPGEIAIRgpqvmagywnrpdetakvmTPDGFFKSGDV 443
Cdd:PRK08308 247 E-AGCVSICPDM--KSHLDLGNPLPHVSVS-AGSD-----ENAPEEIVVK-------------------MGDKEIFTKDL 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 444 GVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPaLTTEALMAYCKK 523
Cdd:PRK08308 299 GYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEE-IDPVQLREWCIQ 377
|
330 340 350
....*....|....*....|....*....|
gi 979216869 524 EFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:PRK08308 378 HLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
170-555 |
2.77e-22 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 99.68 E-value: 2.77e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 170 VKKLVPPYRLPGATPfrTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIAnilqndawSQPALRKAP 249
Cdd:PRK07445 87 VLNLVQPDQIWGLDQ--LKLSHPPPLPSQGILPNLETGWIMIPTGGSSGQIRFAIHTWETLTA--------SVQGFQRYF 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 250 QIDALVTVCALPLYHILALNACM--------LMGARWGAMNLLIPNPRDVDGFVKELsryrvnffpgVNTLFNALL-NHP 320
Cdd:PRK07445 157 QLQQVNSFCVLPLYHVSGLMQFMrsfltggkLVILPYKRLKSGQELPPNPSDFFLSL----------VPTQLQRLLqLRP 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 321 GFAAlDFSGlrITLGGGMAVQEVVSTrwMKTTGCPILEGYGLSET-SPSVTLSPptlGAF-TGTIGL--PVPSTDIALlg 396
Cdd:PRK07445 227 QWLA-QFRT--ILLGGAPAWPSLLEQ--ARQLQLRLAPTYGMTETaSQIATLKP---DDFlAGNNSSgqVLPHAQITI-- 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 397 endepvAQGQPGEIAIRGPQVMAGYWnrPDETAkvmtPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIES 476
Cdd:PRK07445 297 ------PANQTGNITIQAQSLALGYY--PQILD----SQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEA 364
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 979216869 477 VVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRD 555
Cdd:PRK07445 365 AILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDPSISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
206-553 |
8.86e-22 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 98.24 E-value: 8.86e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIAnilqndawSQPALRK-----APQIDALVTVCALPLYHILALNACMLMGARwga 280
Cdd:cd17648 95 DLAYAIYTSGTTGKPKGVLVEHGSVVN--------LRTSLSEryfgrDNGDEAVLFFSNYVFDFFVEQMTLALLNGQ--- 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 281 mNLLIPNPRDV---DGFVKELSRYRVNFFPGVNTLfnalLNHPGFAALDfsGLRITLGGGMAVQEVVSTRWMKTTGCPIL 357
Cdd:cd17648 164 -KLVVPPDEMRfdpDRFYAYINREKVTYLSGTPSV----LQQYDLARLP--HLKRVDAAGEEFTAPVFEKLRSRFAGLII 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPSVTLSP-PTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDG 436
Cdd:cd17648 237 NAYGPTETTVTNHKRFfPGDQRFDKSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNP 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 437 F--------------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEAVK 502
Cdd:cd17648 317 FqteqerargrnarlYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKEDASQAQSRI 396
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 503 L-----FVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRREL 553
Cdd:cd17648 397 QkylvgYYLPEPGHVPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
187-492 |
2.88e-21 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 97.14 E-value: 2.88e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 187 TALRDGARQPFQPAAlnHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqPALRKAPQIDALVTV-CA-LPLYH 264
Cdd:PRK05851 136 TAAHTNRSASLTPPD--SGGPAVLQGTAGSTGTPRTAILSPGAVLSNL--------RGLNARVGLDAATDVgCSwLPLYH 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 265 ILALnACMLMGARWGAMNLLIPNprdvDGF-------VKELSRYRVNFFPGVNTLFNALLNHPG-FAALDFSGLRITLGG 336
Cdd:PRK05851 206 DMGL-AFLLTAALAGAPLWLAPT----TAFsaspfrwLSWLSDSRATLTAAPNFAYNLIGKYARrVSDVDLGALRVALNG 280
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 337 GMAVQEVVSTRWMKTTG------CPILEGYGLSETSPSVTLSPPTLGAFTGTI--------------GLPVPSTDIALL- 395
Cdd:PRK05851 281 GEPVDCDGFERFATAMApfgfdaGAAAPSYGLAESTCAVTVPVPGIGLRVDEVttddgsgarrhavlGNPIPGMEVRISp 360
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 396 GENDEPVAQGQPGEIAIRGPQVMAGYWNRPDetakvMTPDGFFKSGDVGVMDEHGFVrIVDRKKDMILVSGFNVYPNEIE 475
Cdd:PRK05851 361 GDGAAGVAGREIGEIEIRGASMMSGYLGQAP-----IDPDDWFPTGDLGYLVDGGLV-VCGRAKELITVAGRNIFPTEIE 434
|
330
....*....|....*..
gi 979216869 476 SVVAAHPGVIECACVGV 492
Cdd:PRK05851 435 RVAAQVRGVREGAVVAV 451
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
473-547 |
3.91e-21 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 87.21 E-value: 3.91e-21
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 473 EIESVVAAHPGVIECACVGVPDSNSGEAVKLFVVRRD-PALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGK 547
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPgVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
343-554 |
6.18e-21 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 96.56 E-value: 6.18e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 343 VVSTRWMKTTGCPILEgyglseTSPSVTLSPPTLGaftgTIGLPVPSTDIALLGEND-EPVAQGQPGEIAIRGP------ 415
Cdd:PRK10524 383 VIDNYWQTETGWPILA------IARGVEDRPTRLG----SPGVPMYGYNVKLLNEVTgEPCGPNEKGVLVIEGPlppgcm 452
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 416 QVMAG--------YWNRPDETAkvmtpdgfFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEC 487
Cdd:PRK10524 453 QTVWGdddrfvktYWSLFGRQV--------YSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEV 524
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 979216869 488 ACVGVPDSNSGEAVKLFVVRRDPALTTEA---------LMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR 554
Cdd:PRK10524 525 AVVGVKDALKGQVAVAFVVPKDSDSLADRearlalekeIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQ 600
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
210-478 |
8.05e-21 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 96.27 E-value: 8.05e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 210 LQYTGGTTGVSKGATLTHRNVIANILqndAWSQPALRKAPQIDALVTVCALPLYHILALNACMLMGARWGAMnLLIPNPR 289
Cdd:cd05933 155 LIYTSGTTGMPKGVMLSHDNITWTAK---AASQHMDLRPATVGQESVVSYLPLSHIAAQILDIWLPIKVGGQ-VYFAQPD 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 290 DVDG-FVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLR---------ITLGGGMAVQE-----VVSTRWMKT--- 351
Cdd:cd05933 231 ALKGtLVKTLREVRPTAFMGVPRVWEKIQEKMKAVGAKSGTLKrkiaswakgVGLETNLKLMGgespsPLFYRLAKKlvf 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 352 ----------------TGC----------------PILEGYGLSETSPSVTLSPPtlGAFT-GTIGLPVPSTDIALlgen 398
Cdd:cd05933 311 kkvrkalgldrcqkffTGAapisretlefflslniPIMELYGMSETSGPHTISNP--QAYRlLSCGKALPGCKTKI---- 384
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 399 DEPVAQGQpGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS-GFNVYPNEIESV 477
Cdd:cd05933 385 HNPDADGI-GEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGKLDEDGFLYITGRIKELIITAgGENVPPVPIEDA 463
|
.
gi 979216869 478 V 478
Cdd:cd05933 464 V 464
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
182-484 |
2.31e-20 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 95.18 E-value: 2.31e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 182 ATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANIlqndawsqPALRKA-PQIDAL-VTVCA 259
Cdd:PLN02387 227 VSSFSEVEKLGKENPVDPDLPSPNDIAVIMYTSGSTGLPKGVMMTHGNIVATV--------AGVMTVvPKLGKNdVYLAY 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 260 LPLYHILALNACMLMGARWGAM----------------------------NLLIPNPRDVDGfVKELSRYRVNFFPG-VN 310
Cdd:PLN02387 299 LPLAHILELAAESVMAAVGAAIgygspltltdtsnkikkgtkgdasalkpTLMTAVPAILDR-VRDGVRKKVDAKGGlAK 377
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 311 TLFN-------ALLNHPGFAA----------LDFSGLRITLGG-------GMAVQEVVSTRWMKTT-GCPILEGYGLSET 365
Cdd:PLN02387 378 KLFDiaykrrlAAIEGSWFGAwglekllwdaLVFKKIRAVLGGrirfmlsGGAPLSGDTQRFINIClGAPIGQGYGLTET 457
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 366 SPSVTLSPPTLGAfTGTIGLPVPSTDIALLG-------ENDEPVAQGqpgEIAIRGPQVMAGYWNRPDETAKVMTPDG-- 436
Cdd:PLN02387 458 CAGATFSEWDDTS-VGRVGPPLPCCYVKLVSweeggylISDKPMPRG---EIVIGGPSVTLGYFKNQEKTDEVYKVDErg 533
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 437 --FFKSGDVGVMDEHGFVRIVDRKKDMI-LVSGFNVYPNEIESVVAAHPGV 484
Cdd:PLN02387 534 mrWFYTGDIGQFHPDGCLEIIDRKKDIVkLQHGEYVSLGKVEAALSVSPYV 584
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
46-556 |
7.24e-20 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 92.49 E-value: 7.24e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYP--------VAVAAALragftvVNVNpLYTPrELEHQLR 117
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFValwlglakIGVETAL------INSN-LRLE-SLLHCIT 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 118 DSGAEAIVVlenfattlqqviartdvrhvvvaslgDLLGPVRgmavnfvarRVKKLVPPYRLPGatpfrtalrdgarqpf 197
Cdd:cd05939 73 VSKAKALIF--------------------------NLLDPLL---------TQSSTEPPSQDDV---------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 198 qpaalNHDDVAFLQYTGGTTGVSKGATLTHrnviANILQNDAWSQPALRKAPqiDALVTVCaLPLYH----ILALNACML 273
Cdd:cd05939 102 -----NFRDKLFYIYTSGTTGLPKAAVIVH----SRYYRIAAGAYYAFGMRP--EDVVYDC-LPLYHsaggIMGVGQALL 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 274 MGarwgaMNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQevVSTRWMKTTG 353
Cdd:cd05939 170 HG-----STVVIRKKFSASNFWDDCVKYNCTIVQYIGEICRYLLAQPPSEEEQKHNVRLAVGNGLRPQ--IWEQFVRRFG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 354 CP-ILEGYGLSETSPSVTLSPPTLGA--FTGTIGLPV-PstdIALLGENDE-------------PVAQGQPGE----IAI 412
Cdd:cd05939 243 IPqIGEFYGATEGNSSLVNIDNHVGAcgFNSRILPSVyP---IRLIKVDEDtgelirdsdglciPCQPGEPGLlvgkIIQ 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 413 RGP-QVMAGYWNRPDETAKVM-----TPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIE 486
Cdd:cd05939 320 NDPlRRFDGYVNEGATNKKIArdvfkKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLED 399
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 979216869 487 CACVGV--PDSnSGEAVKLFVVRRDPALTTEALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:cd05939 400 VVVYGVevPGV-EGRAGMAAIVDPERKVDLDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQKE 470
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
47-491 |
7.43e-20 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 93.26 E-value: 7.43e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 L-ENFATTLQQVIARTD-VRHVVVASlgdllgpVRGMavnfvaRRvkklvppYRLPGATPFRTALRDG----ARQP---- 196
Cdd:cd17641 90 EdEEQVDKLLEIADRIPsVRYVIYCD-------PRGM------RK-------YDDPRLISFEDVVALGraldRRDPglye 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 197 FQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVI---ANILQNDawsqPALRKA--------PQIDALVTVCALPLYHI 265
Cdd:cd17641 150 REVAAGKGEDVAVLCTTSGTTGKPKLAMLSHGNFLghcAAYLAAD----PLGPGDeyvsvlplPWIGEQMYSVGQALVCG 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 266 LALN-----ACMLMGARWGAMNLLIPNPR-------DVDGFVKELSRYRVNFF------------------------PGV 309
Cdd:cd17641 226 FIVNfpeepETMMEDLREIGPTFVLLPPRvwegiaaDVRARMMDATPFKRFMFelgmklglraldrgkrgrpvslwlRLA 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 310 NTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVsTRWMKTTGCPILEGYGLSETSPSVTLSPptlgafTG-----TIG 384
Cdd:cd17641 306 SWLADALLFRPLRDRLGFSRLRSAATGGAALGPDT-FRFFHAIGVPLKQLYGQTELAGAYTVHR------DGdvdpdTVG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 385 LPVPSTDIALLGEndepvaqgqpGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKD-MIL 463
Cdd:cd17641 379 VPFPGTEVRIDEV----------GEILVRSPGVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKENGHLVVIDRAKDvGTT 448
|
490 500
....*....|....*....|....*...
gi 979216869 464 VSGFNVYPNEIESVVAAHPGVIECACVG 491
Cdd:cd17641 449 SDGTRFSPQFIENKLKFSPYIAEAVVLG 476
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
206-477 |
1.62e-19 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 92.39 E-value: 1.62e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNV---IANILQNDAWSQPALRKAPqidalVTVCALPLYHIL--ALNACML-----MG 275
Cdd:PLN02614 224 DICTIMYTSGTTGDPKGVMISNESIvtlIAGVIRLLKSANAALTVKD-----VYLSYLPLAHIFdrVIEECFIqhgaaIG 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 276 ARWGAMNLLIPN------------PRDVD----GFVKELSR--------YRVNF---FPGVNTLFNALLNHPGFAALDFS 328
Cdd:PLN02614 299 FWRGDVKLLIEDlgelkptifcavPRVLDrvysGLQKKLSDggflkkfvFDSAFsykFGNMKKGQSHVEASPLCDKLVFN 378
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 329 --------GLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLG---E 397
Cdd:PLN02614 379 kvkqglggNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESCAGTFVSLPDELDMLGTVGPPVPNVDIRLESvpeM 458
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 398 NDEPVAQGQPGEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMI-LVSGFNVYPNEIES 476
Cdd:PLN02614 459 EYDALASTPRGEICIRGKTLFSGYYKREDLTKEVLI-DGWLHTGDVGEWQPNGSMKIIDRKKNIFkLSQGEYVAVENIEN 537
|
.
gi 979216869 477 V 477
Cdd:PLN02614 538 I 538
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
205-465 |
6.23e-19 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 90.67 E-value: 6.23e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRNVIANILQNDAWSQPALRKAPQIDALVTVcaLPLYHI---LALNACMLMGAR---W 278
Cdd:PLN02861 220 TDICTIMYTSGTTGEPKGVILTNRAIIAEVLSTDHLLKVTDRVATEEDSYFSY--LPLAHVydqVIETYCISKGASigfW 297
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 GA--------MNLLIPN-----PRDVD--------------GFVKELSRYRVNFFPGvnTLFNALLNH---PGFAALDFS 328
Cdd:PLN02861 298 QGdirylmedVQALKPTifcgvPRVYDriytgimqkissggMLRKKLFDFAYNYKLG--NLRKGLKQEeasPRLDRLVFD 375
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 329 GLRITLGG-------GMAVQEVVSTRWMKTTGCPIL-EGYGLSETSPSVTLSPPTLGAFTGTIGLPVPSTDIAL--LGEN 398
Cdd:PLN02861 376 KIKEGLGGrvrlllsGAAPLPRHVEEFLRVTSCSVLsQGYGLTESCGGCFTSIANVFSMVGTVGVPMTTIEARLesVPEM 455
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 399 DEPVAQGQP-GEIAIRGPQVMAGYWNRPDETAKVMTpDGFFKSGDVGVMDEHGFVRIVDRKKDMILVS 465
Cdd:PLN02861 456 GYDALSDVPrGEICLRGNTLFSGYHKRQDLTEEVLI-DGWFHTGDIGEWQPNGAMKIIDRKKNIFKLS 522
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
205-556 |
1.86e-18 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 88.26 E-value: 1.86e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 205 DDVAFLQYTGGTTGVSKGATLTHRN--VIANILQNDAWSQPALRkapqidalvTVCALPLYH----ILALNACmLMGARw 278
Cdd:cd05937 87 DDPAILIYTSGTTGLPKAAAISWRRtlVTSNLLSHDLNLKNGDR---------TYTCMPLYHgtaaFLGACNC-LMSGG- 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 279 gamNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQevVSTRWMKTTGCPIL- 357
Cdd:cd05937 156 ---TLALSRKFSASQFWKDVRDSGATIIQYVGELCRYLLSTPPSPYDRDHKVRVAWGNGLRPD--IWERFRERFNVPEIg 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSE-TSPSVTLS--PPTLGA--FTGTIGLPVPSTDIALLG---ENDEP-----------VAQGQPGEIAIRGP--- 415
Cdd:cd05937 231 EFYAATEgVFALTNHNvgDFGAGAigHHGLIRRWKFENQVVLVKmdpETDDPirdpktgfcvrAPVGEPGEMLGRVPfkn 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 416 -QVMAGYWNRPDETAKVMTPDGF------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECA 488
Cdd:cd05937 311 rEAFQGYLHNEDATESKLVRDVFrkgdiyFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEAN 390
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 979216869 489 CVGVP----DSNSGEAVkLFVVRRDPALTTE---ALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:cd05937 391 VYGVKvpghDGRAGCAA-ITLEESSAVPTEFtksLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLRDE 464
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
22-553 |
4.53e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 88.69 E-value: 4.53e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 22 YP---SLAHLLDESFATFAARNAYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGF 98
Cdd:PRK05691 3716 YPleqSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGA 3795
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 99 TVVNVNPLYTPRELEHQLRDSGAEAIVVLenfATTLQQVIArtdvrhvvvasLGDLLGpvrgmavnfVARRVKKLVPPYR 178
Cdd:PRK05691 3796 GYLPLDPGLPAQRLQRIIELSRTPVLVCS---AACREQARA-----------LLDELG---------CANRPRLLVWEEV 3852
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 179 LPGATPFRTALRDGARqpfqpaalnhDDVAFLQYTGGTTGVSKGATLTHRNVIANIL---------QNDAWSQPAlrkAP 249
Cdd:PRK05691 3853 QAGEVASHNPGIYSGP----------DNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLskvpylalsEADVIAQTA---SQ 3919
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 250 QIDALV-TVCALPLYhilalnacmlmgarwGAMNLLIPN--PRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGfAALD 326
Cdd:PRK05691 3920 SFDISVwQFLAAPLF---------------GARVEIVPNaiAHDPQGLLAHVQAQGITVLESVPSLIQGMLAEDR-QALD 3983
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 327 fsGLRITLGGGMAVQEVVSTRWMKT-TGCPILEGYGLSETSPSVTLSPPTLGAFTGT---IGLPVPSTDIALLGENDEPV 402
Cdd:PRK05691 3984 --GLRWMLPTGEAMPPELARQWLQRyPQIGLVNAYGPAECSDDVAFFRVDLASTRGSylpIGSPTDNNRLYLLDEALELV 4061
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 AQGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGF-------FKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIE 475
Cdd:PRK05691 4062 PLGAVGELCVAGTGVGRGYVGDPLRTALAFVPHPFgapgerlYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIE 4141
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 476 SVVAAHPGVIECAcVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKK----EFTGYKKPKYIEFRDSLPKSNIGKILRR 551
Cdd:PRK05691 4142 ARLHEQAEVREAA-VAVQEGVNGKHLVGYLVPHQTVLAQGALLERIKQrlraELPDYMVPLHWLWLDRLPLNANGKLDRK 4220
|
..
gi 979216869 552 EL 553
Cdd:PRK05691 4221 AL 4222
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
75-503 |
4.77e-17 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 84.10 E-value: 4.77e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 75 RVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEhqlrdsgaeaivvlenfattlqQVIARTDVRHVVVAS-LGD 153
Cdd:PRK06334 69 HIGIMMPASAGAYIAYFATLLSGKIPVMINWSQGLREVT----------------------ACANLVGVTHVLTSKqLMQ 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 154 LLGPVRGMAVNFVA--------RRVKKLVPPYRLP--GATPFRTALRdgarqPFQPAALNHDDVAFLQYTGGTTGVSKGA 223
Cdd:PRK06334 127 HLAQTHGEDAEYPFsliymeevRKELSFWEKCRIGiyMSIPFEWLMR-----WFGVSDKDPEDVAVILFTSGTEKLPKGV 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 224 TLTHRNVIANilqndawsQPALRK--APQIDAlVTVCALPLYHILALNACMLMGARWGAMNLLIPNPRDVDGFVKELSRY 301
Cdd:PRK06334 202 PLTHANLLAN--------QRACLKffSPKEDD-VMMSFLPPFHAYGFNSCTLFPLLSGVPVVFAYNPLYPKKIVEMIDEA 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 302 RVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGMAVQEVVSTRWMKTTGCPIL-EGYGLSETSPSVTLSPPTLGAFT 380
Cdd:PRK06334 273 KVTFLGSTPVFFDYILKTAKKQESCLPSLRFVVIGGDAFKDSLYQEALKTFPHIQLrQGYGTTECSPVITINTVNSPKHE 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 381 GTIGLPVPSTDIALLGENDE-PVAQGQPGEIAIRGPQVMAGYW-NRPDETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRK 458
Cdd:PRK06334 353 SCVGMPIRGMDVLIVSEETKvPVSSGETGLVLTRGTSLFSGYLgEDFGQGFVELGGETWYVTGDLGYVDRHGELFLKGRL 432
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|.
gi 979216869 459 KDMILVSGFNVYPNEIESVVAAH------PGVIECACVGVPdsnsGEAVKL 503
Cdd:PRK06334 433 SRFVKIGAEMVSLEALESILMEGfgqnaaDHAGPLVVCGLP----GEKVRL 479
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
41-556 |
7.89e-17 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 83.41 E-value: 7.89e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 41 AYACMGRTMTYAELDTYSRHLGAWLQLRGLRKGARVAV---MMPNVLqypVAVAAALRAGFTVVNVNpLYTPRELEHQLR 117
Cdd:PRK04813 20 AYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVfghMSPEML---ATFLGAVKAGHAYIPVD-VSSPAERIEMII 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 118 D-SGAEAIVVLENFATTLqqviarTDVRHVVVASLGDLLgpvrgmavnfvarrvkklvppyrLPGATPFRTAlrdgarqp 196
Cdd:PRK04813 96 EvAKPSLIIATEELPLEI------LGIPVITLDELKDIF-----------------------ATGNPYDFDH-------- 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 197 fqpaALNHDDVAFLQYTGGTTGVSKGATLTHrnviANILQNDAW--SQPALRKAPQIdalvtvCALPLYH----ILALNA 270
Cdd:PRK04813 139 ----AVKGDDNYYIIFTSGTTGKPKGVQISH----DNLVSFTNWmlEDFALPEGPQF------LNQAPYSfdlsVMDLYP 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 271 CMLMGarwGAMNLLipnPRDVDGFVKELsryrvnfFPGVNTL-FNALLNHPGFAAL-----DFSG-----LRITL--GGG 337
Cdd:PRK04813 205 TLASG---GTLVAL---PKDMTANFKQL-------FETLPQLpINVWVSTPSFADMclldpSFNEehlpnLTHFLfcGEE 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 338 MAVQ--EVVSTRWMKTTgcpILEGYGLSETSPSVT---LSPPTLGAFTG-TIGLPVPSTDIALLGENDEPVAQGQPGEIA 411
Cdd:PRK04813 272 LPHKtaKKLLERFPSAT---IYNTYGPTEATVAVTsieITDEMLDQYKRlPIGYAKPDSPLLIIDEEGTKLPDGEQGEIV 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYWNRPDETAKVM-TPDG--FFKSGDVGVMDEhGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIEca 488
Cdd:PRK04813 349 ISGPSVSKGYLNNPEKTAEAFfTFDGqpAYHTGDAGYLED-GLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVES-- 425
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 489 CVGVPdSNSGEAVKLFV---------VRRDPALTTeALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDE 556
Cdd:PRK04813 426 AVVVP-YNKDHKVQYLIayvvpkeedFEREFELTK-AIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKALIEE 500
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
47-561 |
9.22e-17 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 83.59 E-value: 9.22e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVV 126
Cdd:PLN03052 207 NRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLKISKAKAIFT 286
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfattlQQVIARTDVRHvvvaslgdllgPVRGMAVNfvARRVKKLVPPYRlpgATPFRTALRDG-------------A 193
Cdd:PLN03052 287 --------QDVIVRGGKSI-----------PLYSRVVE--AKAPKAIVLPAD---GKSVRVKLREGdmswddflarangL 342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 194 RQP--FQPAALNHDDVAFLQYTGGTTGVSKGATLTHRNVIANilQNDAWSQPALRKApQIDALVTVCALPLYHILaLNAC 271
Cdd:PLN03052 343 RRPdeYKAVEQPVEAFTNILFSSGTTGEPKAIPWTQLTPLRA--AADAWAHLDIRKG-DIVCWPTNLGWMMGPWL-VYAS 418
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 272 MLMGArwgAMNLLIPNPRDvDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRI--TLGGGMAVQEVV---ST 346
Cdd:PLN03052 419 LLNGA---TLALYNGSPLG-RGFAKFVQDAKVTMLGTVPSIVKTWKNTNCMAGLDWSSIRCfgSTGEASSVDDYLwlmSR 494
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 347 RWMKttgcPILEGYGLSETSPS-VT---LSPPTLGAFTGtiglPVPSTDIALLGENDEPVAQGQP--GEIAIrGPQVM-A 419
Cdd:PLN03052 495 AGYK----PIIEYCGGTELGGGfVTgslLQPQAFAAFST----PAMGCKLFILDDSGNPYPDDAPctGELAL-FPLMFgA 565
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 GYWNRPDETAKVmtpdgFFKS------------GDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVV-AAHPGVIE 486
Cdd:PLN03052 566 SSTLLNADHYKV-----YFKGmpvfngkilrrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLE 640
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 487 CACVGVPDSNSG-EAVKLFVVRRDPALTTEAL----MAYCK---KEFTGYKKPKYIEFRDSLPKSNIGKILRRELRDEHG 558
Cdd:PLN03052 641 TAAIGVPPPGGGpEQLVIAAVLKDPPGSNPDLnelkKIFNSaiqKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQQLA 720
|
...
gi 979216869 559 QTA 561
Cdd:PLN03052 721 QEL 723
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
46-540 |
4.44e-16 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 81.18 E-value: 4.44e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLG-AWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAI 124
Cdd:cd05938 3 GETYTYRDVDRRSNQAArALLAHAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKVL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 VVLENFATTLQQVIA--RTDVRHVVVASLGDllgPVRGMA--VNFVARRVKKLVPPYrlpgatpfrtaLRdgarqpfqpA 200
Cdd:cd05938 83 VVAPELQEAVEEVLPalRADGVSVWYLSHTS---NTEGVIslLDKVDAASDEPVPAS-----------LR---------A 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 201 ALNHDDVAFLQYTGGTTGVSKGATLTHRNVIA--NILQndawsqpaLRKAPQIDALVTVcaLPLYH----ILALNACMLM 274
Cdd:cd05938 140 HVTIKSPALYIYTSGTTGLPKAARISHLRVLQcsGFLS--------LCGVTADDVIYIT--LPLYHssgfLLGIGGCIEL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 275 GArwgamNLLIPNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFSGLRITLGGGmaVQEVVSTRWMKTTG- 353
Cdd:cd05938 210 GA-----TCVLKPKFSASQFWDDCRKHNVTVIQYIGELLRYLCNQPQSPNDRDHKVRLAIGNG--LRADVWREFLRRFGp 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 354 CPILEGYGLSETSPSvtlspptLGAFTGTIG----------LPVPSTDIALLGENDEP----------VAQGQPGEIA-- 411
Cdd:cd05938 283 IRIREFYGSTEGNIG-------FFNYTGKIGavgrvsylykLLFPFELIKFDVEKEEPvrdaqgfcipVAKGEPGLLVak 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 412 IRGPQVMAGYWNRPDETAK-----VMTP-DGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVI 485
Cdd:cd05938 356 ITQQSPFLGYAGDKEQTEKkllrdVFKKgDVYFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQ 435
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 979216869 486 ECACVGVPDSN-SGEAVKLFVVRRDP-ALTTEALMAYCKKEFTGYKKPKYIEFRDSL 540
Cdd:cd05938 436 EVNVYGVTVPGhEGRIGMAAVKLKPGhEFDGKKLYQHVREYLPAYARPRFLRIQDSL 492
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
209-550 |
9.83e-16 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 80.56 E-value: 9.83e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 209 FLQYTGGTTGVSKGATLTHRNVIANILQNdaWSQPALRKAPQIDALVTVCALPLYHiLALNACMLMGARWGAMNLLIPNP 288
Cdd:PTZ00237 258 YILYTSGTTGNSKAVVRSNGPHLVGLKYY--WRSIIEKDIPTVVFSHSSIGWVSFH-GFLYGSLSLGNTFVMFEGGIIKN 334
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 289 RDV-DGFVKELSRYRVN-FFPGVNTLFNALLNHPGFAAL----DFSGLRITLGGGMAVQEVVSTRWMKTTGCPILEGYGL 362
Cdd:PTZ00237 335 KHIeDDLWNTIEKHKVThTLTLPKTIRYLIKTDPEATIIrskyDLSNLKEIWCGGEVIEESIPEYIENKLKIKSSRGYGQ 414
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 363 SETSPSVTLSPPTLGAFTGTIGLPVPSTDIALLGENDEPVAQGQPGEIAIR--GPQVMAGYWNRPDETAKVMTPD--GFF 438
Cdd:PTZ00237 415 TEIGITYLYCYGHINIPYNATGVPSIFIKPSILSEDGKELNVNEIGEVAFKlpMPPSFATTFYKNDEKFKQLFSKfpGYY 494
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 439 KSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGEA-VKLFVVRRDPALTTEAL 517
Cdd:PTZ00237 495 NSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNVpIGLLVLKQDQSNQSIDL 574
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 979216869 518 MAY-------CKKEFTGYKKPKYIEFRDSLPKSNIGKILR 550
Cdd:PTZ00237 575 NKLkneinniITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
14-484 |
2.45e-12 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 69.41 E-value: 2.45e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 14 PATVDPDRYP--SLAHLLDESFATFAARNAYACMGR--------------------TMTYAELDTYSRHLGAWLQL-RGL 70
Cdd:cd17632 11 EAVTEAIRRPglRLAQIIATVMTGYADRPALGQRATelvtdpatgrttlrllprfeTITYAELWERVGAVAAAHDPeQPV 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 71 RKGARVAVMMPNVLQYPVAVAAALRAGftVVNVnPLYT---PRELEHQLRDSGAEAIVV-LENFATTLQQVIARTDVRHV 146
Cdd:cd17632 91 RPGDFVAVLGFTSPDYATVDLALTRLG--AVSV-PLQAgasAAQLAPILAETEPRLLAVsAEHLDLAVEAVLEGGTPPRL 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 147 VVASLGDLLGPVRGmAVNFVARRVKKLVPPYRLpgATPFRTALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSKGATLT 226
Cdd:cd17632 168 VVFDHRPEVDAHRA-ALESARERLAAVGIPVTT--LTLIAVRGRDLPPAPLFRPEPDDDPLALLIYTSGSTGTPKGAMYT 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 227 HRNVIANILQNDAWsqpalrKAPQIDALVTVCALPLYHILALNACMLMGARwGAMNLLIPNPrDVDGFVKELSRYR---V 303
Cdd:cd17632 245 ERLVATFWLKVSSI------QDIRPPASITLNFMPMSHIAGRISLYGTLAR-GGTAYFAAAS-DMSTLFDDLALVRpteL 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 304 NFFPGVNTL----FNALLNHPGFAALDFSGL---------RITLGGGMAVQEVVS-------TRWMKTT-GCPILEGYGL 362
Cdd:cd17632 317 FLVPRVCDMlfqrYQAELDRRSVAGADAETLaervkaelrERVLGGRLLAAVCGSaplsaemKAFMESLlDLDLHDGYGS 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 363 SETSpSVTLSpptlgaftGTIGLPvPSTDIAL-----LG--ENDEPVAQGqpgEIAIRGPQVMAGYWNRPDETAKVMTPD 435
Cdd:cd17632 397 TEAG-AVILD--------GVIVRP-PVLDYKLvdvpeLGyfRTDRPHPRG---ELLVKTDTLFPGYYKRPEVTAEVFDED 463
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 979216869 436 GFFKSGDvgVMDEHGFVRI--VDRKKDMI-LVSGFNVYPNEIESVVAAHPGV 484
Cdd:cd17632 464 GFYRTGD--VMAELGPDRLvyVDRRNNVLkLSQGEFVTVARLEAVFAASPLV 513
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
48-561 |
9.22e-12 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 67.62 E-value: 9.22e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIVVL 127
Cdd:PLN02654 120 SLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITC 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 128 ENFATTLQQVIARTDVRHVVVASLGDllGPVRGMAVNFVARRVKKLVppyrlpgATPFRTAlRDGARQPFQPAA------ 201
Cdd:PLN02654 200 NAVKRGPKTINLKDIVDAALDESAKN--GVSVGICLTYENQLAMKRE-------DTKWQEG-RDVWWQDVVPNYptkcev 269
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 202 --LNHDDVAFLQYTGGTTGVSKGATLTHRNVIanILQNDAWSQPALRKAPQIDALVTVCALPLYHILALNACMLMGArwg 279
Cdd:PLN02654 270 ewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM--VYTATTFKYAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGA--- 344
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 280 amNLLI----PNPRDVDGFVKELSRYRVNFFPGVNTLFNALLNHPGFAALDFS--GLRItLGggmAVQEVVST---RW-- 348
Cdd:PLN02654 345 --TVLVfegaPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSrkSLRV-LG---SVGEPINPsawRWff 418
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 349 --MKTTGCPILEGYGLSETSPSVTLSPPtlGAFT---GTIGLPVPSTDIALLGENDEPVAQGQPGEIAIRG--PQVMAGY 421
Cdd:PLN02654 419 nvVGDSRCPISDTWWQTETGGFMITPLP--GAWPqkpGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKswPGAFRTL 496
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 422 WNRPD--ETAKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGVPDSNSGE 499
Cdd:PLN02654 497 YGDHEryETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQ 576
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 979216869 500 AVKLFVVRRDPALTTE----ALMAYCKKEFTGYKKPKYIEFRDSLPKSNIGKILRRELR-------DEHGQTA 561
Cdd:PLN02654 577 GIYAFVTLVEGVPYSEelrkSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRkiasrqlDELGDTS 649
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
50-524 |
5.23e-10 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 62.29 E-value: 5.23e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 50 TYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPlytprelehqlrDSGAEAivVLEN 129
Cdd:cd05943 100 TWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSP------------DFGVPG--VLDR 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 130 FATTLQQVI--------------ARTDVRHVVvASLGDLLGPVrgmAVNFVARRVKKLVPPYrlPGATPFRTALRDGARQ 195
Cdd:cd05943 166 FGQIEPKVLfavdaytyngkrhdVREKVAELV-KGLPSLLAVV---VVPYTVAAGQPDLSKI--AKALTLEDFLATGAAG 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 196 PFQPAALNHDDVAFLQYTGGTTGVSK-------GATLTHRNviANILQNDawsqpaLRKApqiDAL--VTVCALPLYHIL 266
Cdd:cd05943 240 ELEFEPLPFDHPLYILYSSGTTGLPKcivhgagGTLLQHLK--EHILHCD------LRPG---DRLfyYTTCGWMMWNWL 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 267 AlnACMLMGArwgAMNLLIPNP--RDVDGFVKELSRYRVNFFpGVN-TLFNALLN---HPGfAALDFSGLRiTLGggmav 340
Cdd:cd05943 309 V--SGLAVGA---TIVLYDGSPfyPDTNALWDLADEEGITVF-GTSaKYLDALEKaglKPA-ETHDLSSLR-TIL----- 375
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 341 qevvstrwmkTTGCPIL-EGYGL--SETSPSVTLSP---------------PTLGAFTGTIGLPVPSTDIALLGENDEPV 402
Cdd:cd05943 376 ----------STGSPLKpESFDYvyDHIKPDVLLASisggtdiiscfvggnPLLPVYRGEIQCRGLGMAVEAFDEEGKPV 445
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 403 AqGQPGEIAIRG--PQVMAGYWNRPDET----AKVMTPDGFFKSGDVGVMDEHGFVRIVDRKKDMILVSGFNVYPNEIES 476
Cdd:cd05943 446 W-GEKGELVCTKpfPSMPVGFWNDPDGSryraAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYR 524
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 979216869 477 VVAAHPGVIECACVGVPDSNSGEAVKLFVVRRDPALTTEALMAYCKKE 524
Cdd:cd05943 525 VVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDELRKRIRST 572
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
46-556 |
8.33e-09 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 57.96 E-value: 8.33e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 46 GRTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELEHQLRDSGAEAIV 125
Cdd:PRK09029 26 DEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLLEELLPSLTLDFAL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 126 VLENFATtlqqviartdvrhvvvaslgdllgpvrgmavnfvarrvkklvppyrLPGATPFRTALRDGArqpfQPAALNHD 205
Cdd:PRK09029 106 VLEGENT----------------------------------------------FSALTSLHLQLVEGA----HAVAWQPQ 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIAN---IL------QNDAWsqpalrkapqidaLVTvcaLPLYHI---------LA 267
Cdd:PRK09029 136 RLATMTLTSGSTGLPKAAVHTAQAHLASaegVLslmpftAQDSW-------------LLS---LPLFHVsgqgivwrwLY 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 268 LNACMLMgarwgamnllipnpRDVDGFVKELSRyrVNFFPGVNTLFNALLNHPGFA-ALDfsglRITLGGGMAVQEVvsT 346
Cdd:PRK09029 200 AGATLVV--------------RDKQPLEQALAG--CTHASLVPTQLWRLLDNRSEPlSLK----AVLLGGAAIPVEL--T 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 347 RWMKTTG----CpileGYGLSETSPSVTlspptlgA----FTGTIGLPVPSTDIALLGendepvaqgqpGEIAIRGPQVM 418
Cdd:PRK09029 258 EQAEQQGircwC----GYGLTEMASTVC-------AkradGLAGVGSPLPGREVKLVD-----------GEIWLRGASLA 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 419 AGYWN----RP--DEtakvmtpDGFFKSGDVGVMDEhGFVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIECACVGV 492
Cdd:PRK09029 316 LGYWRqgqlVPlvND-------EGWFATRDRGEWQN-GELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPV 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 979216869 493 PDSNSGE---AvklfVVRRDPALTTEALMAYCKKEFTGYKKP-KYIEFRDSLPKSNIgKILRRELRDE 556
Cdd:PRK09029 388 ADAEFGQrpvA----VVESDSEAAVVNLAEWLQDKLARFQQPvAYYLLPPELKNGGI-KISRQALKEW 450
|
|
| Dip2 |
cd05905 |
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ... |
48-488 |
1.98e-08 |
|
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.
Pssm-ID: 341231 [Multi-domain] Cd Length: 571 Bit Score: 56.97 E-value: 1.98e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 48 TMTYAELDTYSRHLGAWLQLR-GLRKGARVAVMMPNVLQYPVAVAAALRAGFTVVNVNPLYTPRELehqlrdsgaeaivv 126
Cdd:cd05905 14 TLTWGKLLSRAEKIAAVLQKKvGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQL-------------- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 127 lenfATTLQQVIARTDVRHVVVASLGDLLGPVRGMAVNFVArrvKKLVPPYRLPGATPFRTALRdgaRQPFQP-AALNHD 205
Cdd:cd05905 80 ----GFLLGTCKVRVALTVEACLKGLPKKLLKSKTAAEIAK---KKGWPKILDFVKIPKSKRSK---LKKWGPhPPTRDG 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 206 DVAFLQYTGGTTGVSKGATLTHRNVIA--NILQndawSQPALRKAPQIdalvtVCALPLYHILALNACMLMGARWGAMNL 283
Cdd:cd05905 150 DTAYIEYSFSSDGSLSGVAVSHSSLLAhcRALK----EACELYESRPL-----VTVLDFKSGLGLWHGCLLSVYSGHHTI 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 284 LIP------NPRDvdgFVKELSRYRV-NFFPGVNTLFNALLNHPGFAA------LDFSGLRI------------------ 332
Cdd:cd05905 221 LIPpelmktNPLL---WLQTLSQYKVrDAYVKLRTLHWCLKDLSSTLAslknrdVNLSSLRMcmvpcenrprisscdsfl 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 333 ----TLG-GGMAVQEVVSTR------WMKTTGCPILEGY----GLSE---------TSPSVTLSPptlgaftgtIGLPVP 388
Cdd:cd05905 298 klfqTLGlSPRAVSTEFGTRvnpficWQGTSGPEPSRVYldmrALRHgvvrlderdKPNSLPLQD---------SGKVLP 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 389 STDIALLG-ENDEPVAQGQPGEIAIRGPQVMAGYWNRPDET------------AKVMTPDGFFKSGDVGV---------- 445
Cdd:cd05905 369 GAQVAIVNpETKGLCKDGEIGEIWVNSPANASGYFLLDGETndtfkvfpstrlSTGITNNSYARTGLLGFlrptkctdln 448
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 979216869 446 MDEHGFVRIVDRKKDMILVSGFNVYPNEIE-SVVAAHPGVIECA 488
Cdd:cd05905 449 VEEHDLLFVVGSIDETLEVRGLRHHPSDIEaTVMRVHPYRGRCA 492
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
358-465 |
8.91e-07 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 52.03 E-value: 8.91e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 358 EGYGLSETSPSVTLSPpTLGAFTGTIGLPV-PSTDIALLGENDEPVAQGQP-GEIAIRGPQVMAGYWNRPDETAKVMTPD 435
Cdd:PTZ00342 491 QGYGLTETTGPIFVQH-ADDNNTESIGGPIsPNTKYKVRTWETYKATDTLPkGELLIKSDSIFSGYFLEKEQTKNAFTED 569
|
90 100 110
....*....|....*....|....*....|
gi 979216869 436 GFFKSGDVGVMDEHGFVRIVDRKKDMILVS 465
Cdd:PTZ00342 570 GYFKTGDIVQINKNGSLTFLDRSKGLVKLS 599
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
344-511 |
3.04e-06 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 49.76 E-value: 3.04e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 344 VSTRWmkttGCPILEGYGLSETSPSVTLSPPTLGaftgtiGLPVPStDIALL----GENDEPVAQGQPGEIAIrgpqvma 419
Cdd:COG1541 223 IEERW----GIKAYDIYGLTEVGPGVAYECEAQD------GLHIWE-DHFLVeiidPETGEPVPEGEEGELVV------- 284
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 420 gywnrpdeTAkvMTPDGF----FKSGDVGVMD----EHG-----FVRIVDRKKDMILVSGFNVYPNEIESVVAAHPGVIE 486
Cdd:COG1541 285 --------TT--LTKEAMplirYRTGDLTRLLpepcPCGrthprIGRILGRADDMLIIRGVNVFPSQIEEVLLRIPEVGP 354
|
170 180
....*....|....*....|....*
gi 979216869 487 CACVGVPDSNSGEAVKLFVVRRDPA 511
Cdd:COG1541 355 EYQIVVDREGGLDELTVRVELAPGA 379
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
47-227 |
3.22e-06 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 50.18 E-value: 3.22e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 47 RTMTYAELDTYSRHLGAWLQLRGLRKGARVAVMMPNVLQYPVAV--AAALRAGFTVvnVNPlytprelehqlrDSGAEAi 124
Cdd:PRK03584 113 RELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMlaTASLGAIWSS--CSP------------DFGVQG- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 979216869 125 vVLENFattlQQV-----------------IARTDVRHVVVASLGDLLGPVrgmavnfVARRVKKLVPPYRLPGATPFRT 187
Cdd:PRK03584 178 -VLDRF----GQIepkvliavdgyryggkaFDRRAKVAELRAALPSLEHVV-------VVPYLGPAAAAAALPGALLWED 245
|
170 180 190 200
....*....|....*....|....*....|....*....|....*..
gi 979216869 188 ALRDGARQPFQPAALNHDDVAFLQYTGGTTGVSK-------GATLTH 227
Cdd:PRK03584 246 FLAPAEAAELEFEPVPFDHPLWILYSSGTTGLPKcivhghgGILLEH 292
|
|
| PaaK |
cd05913 |
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ... |
453-484 |
1.79e-03 |
|
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.
Pssm-ID: 341239 [Multi-domain] Cd Length: 425 Bit Score: 41.07 E-value: 1.79e-03
10 20 30
....*....|....*....|....*....|..
gi 979216869 453 RIVDRKKDMILVSGFNVYPNEIESVVAAHPGV 484
Cdd:cd05913 317 RITGRSDDMLIIRGVNVFPSQIEDVLLKIPGL 348
|
|
| |
