NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|145334351|ref|NP_001078557|]
View 

magnesium transporter 7 [Arabidopsis thaliana]

Protein Classification

CorA family magnesium transporter( domain architecture ID 10191269)

CorA family magnesium transporter, belonging to the CorA metal ion transporter (MIT) family, mediates the influx of magnesium ions, similar to mitochondrial inner membrane magnesium transporter MRS2

CATH:  1.20.58.340
Gene Ontology:  GO:0015095|GO:1903830|GO:0016020
PubMed:  17619822|18927203|16720382
SCOP:  4000404|4000464
TCDB:  1.A.35

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 29-368 1.57e-113

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213357  Cd Length: 323  Bit Score: 333.44  E-value: 1.57e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351  29 WISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNL-FYPSAILGRERAIVLNLEHIKAIITAEEVLI---RDSSDEN 104
Cdd:cd12823    1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTLtSYPPSILVRENAILVNLEHIRAIITADEVLLfdpDGSSSAL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 105 VIPVLEEFQRRLPVGNEAHGvhgdgDLGEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPALDELTLKISSRNLER 184
Cdd:cd12823   81 VSAFLEELQRRLASSNGSES-----ESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLER 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 185 VRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASSSVSVSDEPIWYPT-------SPTIGSMISRASRV 257
Cdd:cd12823  156 LLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESSRKEDDHEEVemlleayLQQVDELLNKLEEL 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 258 slvtvrgddetdveelemllelREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDH 337
Cdd:cd12823  236 ----------------------REYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESP 293

                        330       340       350
                 ....*....|....*....|....*....|.
gi 145334351 338 gYIFKWVVSLTGTFCIVLFVIILSYARFRGL 368
Cdd:cd12823  294 -YAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 29-368 1.57e-113

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 333.44  E-value: 1.57e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351  29 WISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNL-FYPSAILGRERAIVLNLEHIKAIITAEEVLI---RDSSDEN 104
Cdd:cd12823    1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTLtSYPPSILVRENAILVNLEHIRAIITADEVLLfdpDGSSSAL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 105 VIPVLEEFQRRLPVGNEAHGvhgdgDLGEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPALDELTLKISSRNLER 184
Cdd:cd12823   81 VSAFLEELQRRLASSNGSES-----ESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLER 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 185 VRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASSSVSVSDEPIWYPT-------SPTIGSMISRASRV 257
Cdd:cd12823  156 LLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESSRKEDDHEEVemlleayLQQVDELLNKLEEL 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 258 slvtvrgddetdveelemllelREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDH 337
Cdd:cd12823  236 ----------------------REYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESP 293

                        330       340       350
                 ....*....|....*....|....*....|.
gi 145334351 338 gYIFKWVVSLTGTFCIVLFVIILSYARFRGL 368
Cdd:cd12823  294 -YAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 288-360 5.55e-03

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 38.06  E-value: 5.55e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145334351  288 DYINIQLDNHRNQLIQlelMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIIL 360
Cdd:pfam01544 220 DEYSSLLSERTNRVMK---VLTVVTAIFLPLTLITGIYGMNFGGLPELEWSYGYPFVLILGLMILLAILLLFY 289
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 29-368 1.57e-113

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 333.44  E-value: 1.57e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351  29 WISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNL-FYPSAILGRERAIVLNLEHIKAIITAEEVLI---RDSSDEN 104
Cdd:cd12823    1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTLtSYPPSILVRENAILVNLEHIRAIITADEVLLfdpDGSSSAL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 105 VIPVLEEFQRRLPVGNEAHGvhgdgDLGEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPALDELTLKISSRNLER 184
Cdd:cd12823   81 VSAFLEELQRRLASSNGSES-----ESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLER 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 185 VRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASSSVSVSDEPIWYPT-------SPTIGSMISRASRV 257
Cdd:cd12823  156 LLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESSRKEDDHEEVemlleayLQQVDELLNKLEEL 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 258 slvtvrgddetdveelemllelREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDH 337
Cdd:cd12823  236 ----------------------REYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESP 293

                        330       340       350
                 ....*....|....*....|....*....|.
gi 145334351 338 gYIFKWVVSLTGTFCIVLFVIILSYARFRGL 368
Cdd:cd12823  294 -YAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 282-367 1.76e-04

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 42.69  E-value: 1.76e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145334351 282 YIDDTEDYINIQLDNHRNQLIQLELMLSAgtvcvsVY---SMIAGIFGMN---IPNTwNHDHGYifkWVVSLtgtFCIVL 355
Cdd:cd12821  206 ELDTLQDIEEVVSSHRGNEIMKTLTVVTT------VFlplTLIGGLWGMNfknMPEL-EWKYGY---LFVLG---LIILS 272

                         90
                 ....*....|..
gi 145334351 356 FVIILSYARFRG 367
Cdd:cd12821  273 TAGLYLYLRRKG 284
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 288-360 5.55e-03

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 38.06  E-value: 5.55e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145334351  288 DYINIQLDNHRNQLIQlelMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIIL 360
Cdd:pfam01544 220 DEYSSLLSERTNRVMK---VLTVVTAIFLPLTLITGIYGMNFGGLPELEWSYGYPFVLILGLMILLAILLLFY 289
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 320-358 9.02e-03

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 37.48  E-value: 9.02e-03
                         10        20        30
                 ....*....|....*....|....*....|....*....
gi 145334351 320 MIAGIFGMNIPNTWnHDHGYIFKWVVSLTGTFCIVLFVI 358
Cdd:cd12827  245 IITGFYGMNVPLPF-ADSPYAFWIILLISLLLSVIVALI 282
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH