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Conserved domains on  [gi|548962168|ref|NP_001271431|]
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cytosolic Fe-S cluster assembly factor NUBP2 isoform 3 [Homo sapiens]

Protein Classification

P-loop NTPase family protein( domain architecture ID 1562424)

P-loop NTPase (nucleoside triphosphate hydrolase) family protein contains two conserved sequence signatures, the Walker A motif (the P-loop proper) and Walker B motif which bind, respectively, the beta and gamma phosphate moieties of the bound nucleotide (typically ATP or GTP), and a Mg(2+) cation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
P-loop_NTPase super family cl38936
P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain ...
 14-59 1.66e-24

P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a hydrophobic residue). The Walker A and B motifs bind the beta-gamma phosphate moiety of the bound nucleotide (typically ATP or GTP) and the Mg2+ cation, respectively. The P-loop NTPases are involved in diverse cellular functions, and they can be divided into two major structural classes: the KG (kinase-GTPase) class which includes Ras-like GTPases and its circularly permutated YlqF-like; and the ASCE (additional strand catalytic E) class which includes ATPase Binding Cassette (ABC), DExD/H-like helicases, 4Fe-4S iron sulfur cluster binding proteins of NifH family, RecA-like F1-ATPases, and ATPases Associated with a wide variety of Activities (AAA). Also included are a diverse set of nucleotide/nucleoside kinase families.


The actual alignment was detected with superfamily member cd02037:

Pssm-ID: 476819 [Multi-domain]  Cd Length: 213  Bit Score: 96.03  E-value: 1.66e-24
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCT 59
Cdd:cd02037  134 GAVVVTTPQEVSLIDVRKAIDMCKKLNIPVLGIVENMSGFVCPHCG 179
PRK14959 super family cl33044
DNA polymerase III subunits gamma and tau; Provisional
 127-190 4.56e-03

DNA polymerase III subunits gamma and tau; Provisional


The actual alignment was detected with superfamily member PRK14959:

Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 37.74  E-value: 4.56e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 548962168 127 TLQPLSRATKGSAPASQRNR------GLGSVPGPCRGRPRQRQP-ESFSPTSPAPGCVAPAALPGwPAVPW 190
Cdd:PRK14959 367 PVESLRPSGGGASAPSGSAAegpasgGAATIPTPGTQGPQGTAPaAGMTPSSAAPATPAPSAAPS-PRVPW 436
 
Name Accession Description Interval E-value
Mrp_NBP35 cd02037
Mrp/NBP35 ATP-binding protein family; Mrp/NBP35 ATP-binding family protein are typically ...
 14-59 1.66e-24

Mrp/NBP35 ATP-binding protein family; Mrp/NBP35 ATP-binding family protein are typically iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes. Members include the eukaryotic nucleotide-binding protein 1 (NUBP1) which is a component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery and the archael [NiFe] hydrogenase maturation protein HypB which is required for nickel insertion into [NiFe] hydrogenase.


Pssm-ID: 349757 [Multi-domain]  Cd Length: 213  Bit Score: 96.03  E-value: 1.66e-24
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCT 59
Cdd:cd02037  134 GAVVVTTPQEVSLIDVRKAIDMCKKLNIPVLGIVENMSGFVCPHCG 179
ParA pfam10609
NUBPL iron-transfer P-loop NTPase; This family contains ATPases involved in plasmid ...
 14-58 3.69e-24

NUBPL iron-transfer P-loop NTPase; This family contains ATPases involved in plasmid partitioning. It also contains the cytosolic Fe-S cluster assembling factor NBP35 which is required for biogenesis and export of both ribosomal subunits.


Pssm-ID: 431392 [Multi-domain]  Cd Length: 246  Bit Score: 95.98  E-value: 3.69e-24
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 548962168   14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:pfam10609 138 GAVIVTTPQDVALLDVRKAIDMFKKVNVPVLGVVENMSYFVCPHC 182
MrpORP NF041136
iron-sulfur cluster carrier protein MrpORP;
14-58 1.10e-21

iron-sulfur cluster carrier protein MrpORP;


Pssm-ID: 469059 [Multi-domain]  Cd Length: 365  Bit Score: 91.42  E-value: 1.10e-21
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:NF041136 141 GAVIVTTPQELALADVRKSINFCRKLNIPILGIVENMSGFVCPHC 185
PRK11670 PRK11670
iron-sulfur cluster carrier protein ApbC;
14-58 2.32e-07

iron-sulfur cluster carrier protein ApbC;


Pssm-ID: 183270 [Multi-domain]  Cd Length: 369  Bit Score: 50.43  E-value: 2.32e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:PRK11670 242 GAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMSMHICSNC 286
Mrp COG0489
Fe-S cluster carrier ATPase, Mrp/ApbC/NBP35 family [Cell cycle control, cell division, ...
 14-57 4.71e-07

Fe-S cluster carrier ATPase, Mrp/ApbC/NBP35 family [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440255 [Multi-domain]  Cd Length: 289  Bit Score: 49.41  E-value: 4.71e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMsgfTCPH 57
Cdd:COG0489  227 GVLLVVRPGKTALDDVRKALEMLEKAGVPVLGVVLNM---VCPK 267
PRK14959 PRK14959
DNA polymerase III subunits gamma and tau; Provisional
 127-190 4.56e-03

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 37.74  E-value: 4.56e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 548962168 127 TLQPLSRATKGSAPASQRNR------GLGSVPGPCRGRPRQRQP-ESFSPTSPAPGCVAPAALPGwPAVPW 190
Cdd:PRK14959 367 PVESLRPSGGGASAPSGSAAegpasgGAATIPTPGTQGPQGTAPaAGMTPSSAAPATPAPSAAPS-PRVPW 436
 
Name Accession Description Interval E-value
Mrp_NBP35 cd02037
Mrp/NBP35 ATP-binding protein family; Mrp/NBP35 ATP-binding family protein are typically ...
 14-59 1.66e-24

Mrp/NBP35 ATP-binding protein family; Mrp/NBP35 ATP-binding family protein are typically iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes. Members include the eukaryotic nucleotide-binding protein 1 (NUBP1) which is a component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery and the archael [NiFe] hydrogenase maturation protein HypB which is required for nickel insertion into [NiFe] hydrogenase.


Pssm-ID: 349757 [Multi-domain]  Cd Length: 213  Bit Score: 96.03  E-value: 1.66e-24
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCT 59
Cdd:cd02037  134 GAVVVTTPQEVSLIDVRKAIDMCKKLNIPVLGIVENMSGFVCPHCG 179
ParA pfam10609
NUBPL iron-transfer P-loop NTPase; This family contains ATPases involved in plasmid ...
 14-58 3.69e-24

NUBPL iron-transfer P-loop NTPase; This family contains ATPases involved in plasmid partitioning. It also contains the cytosolic Fe-S cluster assembling factor NBP35 which is required for biogenesis and export of both ribosomal subunits.


Pssm-ID: 431392 [Multi-domain]  Cd Length: 246  Bit Score: 95.98  E-value: 3.69e-24
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 548962168   14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:pfam10609 138 GAVIVTTPQDVALLDVRKAIDMFKKVNVPVLGVVENMSYFVCPHC 182
MrpORP NF041136
iron-sulfur cluster carrier protein MrpORP;
14-58 1.10e-21

iron-sulfur cluster carrier protein MrpORP;


Pssm-ID: 469059 [Multi-domain]  Cd Length: 365  Bit Score: 91.42  E-value: 1.10e-21
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:NF041136 141 GAVIVTTPQELALADVRKSINFCRKLNIPILGIVENMSGFVCPHC 185
PRK11670 PRK11670
iron-sulfur cluster carrier protein ApbC;
14-58 2.32e-07

iron-sulfur cluster carrier protein ApbC;


Pssm-ID: 183270 [Multi-domain]  Cd Length: 369  Bit Score: 50.43  E-value: 2.32e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHC 58
Cdd:PRK11670 242 GAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMSMHICSNC 286
Mrp COG0489
Fe-S cluster carrier ATPase, Mrp/ApbC/NBP35 family [Cell cycle control, cell division, ...
 14-57 4.71e-07

Fe-S cluster carrier ATPase, Mrp/ApbC/NBP35 family [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440255 [Multi-domain]  Cd Length: 289  Bit Score: 49.41  E-value: 4.71e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 548962168  14 GALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMsgfTCPH 57
Cdd:COG0489  227 GVLLVVRPGKTALDDVRKALEMLEKAGVPVLGVVLNM---VCPK 267
PRK14959 PRK14959
DNA polymerase III subunits gamma and tau; Provisional
 127-190 4.56e-03

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 184923 [Multi-domain]  Cd Length: 624  Bit Score: 37.74  E-value: 4.56e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 548962168 127 TLQPLSRATKGSAPASQRNR------GLGSVPGPCRGRPRQRQP-ESFSPTSPAPGCVAPAALPGwPAVPW 190
Cdd:PRK14959 367 PVESLRPSGGGASAPSGSAAegpasgGAATIPTPGTQGPQGTAPaAGMTPSSAAPATPAPSAAPS-PRVPW 436
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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