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Conserved domains on  [gi|808355127|ref|NP_001293462|]
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DRBM domain-containing protein [Caenorhabditis elegans]

Protein Classification

dsRNA-binding motif domain-containing protein( domain architecture ID 855)

dsRNA-binding motif (DSRM) domain-containing protein may be involved in posttranscriptional gene regulation, for example by preventing the expression of proteins or by mediating RNA localization

Gene Ontology:  GO:0003725
PubMed:  1438302

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DSRM_SF super family cl00054
double-stranded RNA binding motif (DSRM) superfamily; DSRM (also known as dsRBM) is a 65-70 ...
 35-105 5.25e-09

double-stranded RNA binding motif (DSRM) superfamily; DSRM (also known as dsRBM) is a 65-70 amino acid domain that adopts an alpha-beta-beta-beta-alpha fold. It is not sequence specific, but highly specific for double-stranded RNAs (dsRNAs) of various origin and structure. The DSRM domains are found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila Staufen protein, E. coli RNase III, RNase H1, and dsRNA dependent adenosine deaminases. They are involved in numerous cellular mechanisms ranging from localization and transport of messenger RNAs, through maturation and degradation of RNAs, to viral response and signal transduction. Some members harbor tandem DSRMs that act in small RNA biogenesis.


The actual alignment was detected with superfamily member cd19868:

Pssm-ID: 444671  Cd Length: 69  Bit Score: 51.51  E-value: 5.25e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 808355127  35 PKPYNLLRDAVSRSLRWNGMELvmKKEMIGNGSQLSKVILILGDIQEEAEAVGVKQATQMASQRLFKKMHP 105
Cdd:cd19868    1 PSPYQILQECLKRNHGMGDTEL--KFEVINDKHQKHEYTMTVGKHTVTVICKNKKEGKQLAAQAILKKLHP 69
 
Name Accession Description Interval E-value
DSRM_DGCR8_rpt2 cd19868
second double-stranded RNA binding motif of DiGeorge syndrome critical region 8 (DGCR8) and ...
 35-105 5.25e-09

second double-stranded RNA binding motif of DiGeorge syndrome critical region 8 (DGCR8) and similar proteins; DGCR8 is a component of the microprocessor complex that acts as an RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. DGCR8 contains two double-stranded RNA binding motifs (DSRMs). This model describes the second motif. DSRM is not sequence specific, but highly specific for dsRNAs of various origin and structure.


Pssm-ID: 380697  Cd Length: 69  Bit Score: 51.51  E-value: 5.25e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 808355127  35 PKPYNLLRDAVSRSLRWNGMELvmKKEMIGNGSQLSKVILILGDIQEEAEAVGVKQATQMASQRLFKKMHP 105
Cdd:cd19868    1 PSPYQILQECLKRNHGMGDTEL--KFEVINDKHQKHEYTMTVGKHTVTVICKNKKEGKQLAAQAILKKLHP 69
 
Name Accession Description Interval E-value
DSRM_DGCR8_rpt2 cd19868
second double-stranded RNA binding motif of DiGeorge syndrome critical region 8 (DGCR8) and ...
 35-105 5.25e-09

second double-stranded RNA binding motif of DiGeorge syndrome critical region 8 (DGCR8) and similar proteins; DGCR8 is a component of the microprocessor complex that acts as an RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. DGCR8 contains two double-stranded RNA binding motifs (DSRMs). This model describes the second motif. DSRM is not sequence specific, but highly specific for dsRNAs of various origin and structure.


Pssm-ID: 380697  Cd Length: 69  Bit Score: 51.51  E-value: 5.25e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 808355127  35 PKPYNLLRDAVSRSLRWNGMELvmKKEMIGNGSQLSKVILILGDIQEEAEAVGVKQATQMASQRLFKKMHP 105
Cdd:cd19868    1 PSPYQILQECLKRNHGMGDTEL--KFEVINDKHQKHEYTMTVGKHTVTVICKNKKEGKQLAAQAILKKLHP 69
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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