NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1812252035|ref|NP_001344585|]
View 

tubulin-specific chaperone cofactor E-like protein isoform b [Mus musculus]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 337-422 1.34e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


:

Pssm-ID: 340565  Cd Length: 87  Bit Score: 147.39  E-value: 1.34e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 337 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 415
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812252035 416 KIFVESK 422
Cdd:cd17045    81 EILVEPK 87
LRR_9 super family cl25994
Leucine-rich repeat;
191-304 9.83e-10

Leucine-rich repeat;


The actual alignment was detected with superfamily member pfam14580:

Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 57.47  E-value: 9.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 191 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 268
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812252035 269 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 304
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
LRR super family cl34836
Leucine-rich repeat (LRR) protein [Transcription];
73-259 9.96e-10

Leucine-rich repeat (LRR) protein [Transcription];


The actual alignment was detected with superfamily member COG4886:

Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 59.95  E-value: 9.96e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035  73 CAHVSELDLSDNKLQDwheVSKIVSNVPQLEFLNLSSNPLS--LSVLertcaGSFSGVRKLVLNNskaswetvhtilqel 150
Cdd:COG4886   112 LTNLESLDLSGNQLTD---LPEELANLTNLKELDLSNNQLTdlPEPL-----GNLTNLKSLDLSN--------------- 168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 151 peleelflclNDYETVSCPSVCCHSLKLLHITDNNLQDW-TEIRKLgvmfPSLDTLVLANNHLNAIEEPadsLARLfPNL 229
Cdd:COG4886   169 ----------NQLTDLPEELGNLTNLKELDLSNNQITDLpEPLGNL----TNLEELDLSGNQLTDLPEP---LANL-TNL 230

                         170       180       190
                  ....*....|....*....|....*....|
gi 1812252035 230 RSISLHKSGLqswEDIDKLNSFPKLEEVRL 259
Cdd:COG4886   231 ETLDLSNNQL---TDLPELGNLTNLEELDL 257
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 337-422 1.34e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 147.39  E-value: 1.34e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 337 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 415
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812252035 416 KIFVESK 422
Cdd:cd17045    81 EILVEPK 87
LRR_9 pfam14580
Leucine-rich repeat;
191-304 9.83e-10

Leucine-rich repeat;


Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 57.47  E-value: 9.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 191 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 268
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812252035 269 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 304
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
73-259 9.96e-10

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 59.95  E-value: 9.96e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035  73 CAHVSELDLSDNKLQDwheVSKIVSNVPQLEFLNLSSNPLS--LSVLertcaGSFSGVRKLVLNNskaswetvhtilqel 150
Cdd:COG4886   112 LTNLESLDLSGNQLTD---LPEELANLTNLKELDLSNNQLTdlPEPL-----GNLTNLKSLDLSN--------------- 168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 151 peleelflclNDYETVSCPSVCCHSLKLLHITDNNLQDW-TEIRKLgvmfPSLDTLVLANNHLNAIEEPadsLARLfPNL 229
Cdd:COG4886   169 ----------NQLTDLPEELGNLTNLKELDLSNNQITDLpEPLGNL----TNLEELDLSGNQLTDLPEP---LANL-TNL 230

                         170       180       190
                  ....*....|....*....|....*....|
gi 1812252035 230 RSISLHKSGLqswEDIDKLNSFPKLEEVRL 259
Cdd:COG4886   231 ETLDLSNNQL---TDLPELGNLTNLEELDL 257
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 50-113 2.84e-05

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 45.16  E-value: 2.84e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1812252035  50 PSVLVLN--SCGITcagDEREIAAFCaHVSELDLSDNKLQDWHEVSKIVSNVPQLEFLNLSSNPLS 113
Cdd:cd21340   120 NSLRVLNisGNNID---SLEPLAPLR-NLEQLDASNNQISDLEELLDLLSSWPSLRELDLTGNPVC 181
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 175-298 8.28e-04

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 40.54  E-value: 8.28e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 175 SLKLLHITDNNLQDWTEIRKLgvmfPSLDTLVLANNHLNAIEEPADSlarlfpnlrsislhksglqswedidkLNSFPKL 254
Cdd:cd21340   121 SLRVLNISGNNIDSLEPLAPL----RNLEQLDASNNQISDLEELLDL--------------------------LSSWPSL 170

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1812252035 255 EEVRLLGIPLLQPYTTEERrklVVARLPSVSKLNGSVVTDGERE 298
Cdd:cd21340   171 RELDLTGNPVCKKPKYRDK---IILASKSLEVLDGKEITDTERQ 211
Ubiquitin_2 pfam14560
Ubiquitin-like domain; This entry contains ubiquitin-like domains.
 356-419 1.40e-03

Ubiquitin-like domain; This entry contains ubiquitin-like domains.


Pssm-ID: 405277  Cd Length: 83  Bit Score: 37.50  E-value: 1.40e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1812252035 356 EMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 419
Cdd:pfam14560  16 ERRFDKSLTIEELKEKLELITGTPPSSMRLQLYDDDDNL-VAKLDDDDALLGSYGVRDGMRIHV 78
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 337-422 1.34e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 147.39  E-value: 1.34e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 337 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 415
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812252035 416 KIFVESK 422
Cdd:cd17045    81 EILVEPK 87
LRR_9 pfam14580
Leucine-rich repeat;
191-304 9.83e-10

Leucine-rich repeat;


Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 57.47  E-value: 9.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 191 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 268
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812252035 269 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 304
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
73-259 9.96e-10

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 59.95  E-value: 9.96e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035  73 CAHVSELDLSDNKLQDwheVSKIVSNVPQLEFLNLSSNPLS--LSVLertcaGSFSGVRKLVLNNskaswetvhtilqel 150
Cdd:COG4886   112 LTNLESLDLSGNQLTD---LPEELANLTNLKELDLSNNQLTdlPEPL-----GNLTNLKSLDLSN--------------- 168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 151 peleelflclNDYETVSCPSVCCHSLKLLHITDNNLQDW-TEIRKLgvmfPSLDTLVLANNHLNAIEEPadsLARLfPNL 229
Cdd:COG4886   169 ----------NQLTDLPEELGNLTNLKELDLSNNQITDLpEPLGNL----TNLEELDLSGNQLTDLPEP---LANL-TNL 230

                         170       180       190
                  ....*....|....*....|....*....|
gi 1812252035 230 RSISLHKSGLqswEDIDKLNSFPKLEEVRL 259
Cdd:COG4886   231 ETLDLSNNQL---TDLPELGNLTNLEELDL 257
Ubl_TBCE cd17044
ubiquitin-like (Ubl) domain found in tubulin-folding cofactor E (TBCE) and similar proteins; ...
 364-420 4.02e-08

ubiquitin-like (Ubl) domain found in tubulin-folding cofactor E (TBCE) and similar proteins; TBCE, also termed tubulin-specific chaperone E, is a tubulin polymerizing protein involved in the second step of the tubulin folding pathway through cooperating in tubulin heterodimer dissociation both in vivo and in vitro. It may also be implicated in the maintenance of the neuronal microtubule network. Mutations in TBCE gene cause hypoparathyroidism, mental retardation and facial dysmorphism. TBCE contains an N-terminal cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain, a leucine-rich repeat protein-protein interaction domain followed by leucine-rich repeat (LRR) domains, and a C-terminal ubiquitin-like (Ubl) domain. Ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes.


Pssm-ID: 340564  Cd Length: 83  Bit Score: 50.27  E-value: 4.02e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1812252035 364 TVAELKKQLKTLVQLPTSSMLLYYFDHEAPFGPEEMKYSSRALHSFGIRDGDKIFVE 420
Cdd:cd17044    27 TVQKLKGLCERLFKLPASKQRLSYVSSEGPGIEIELDDDLRSLSFYSVEDGDTILVR 83
Ubl_ubiquitin_like cd17039
ubiquitin-like (Ubl) domain found in ubiquitin and ubiquitin-like Ubl proteins; Ubiquitin-like ...
 346-419 2.40e-06

ubiquitin-like (Ubl) domain found in ubiquitin and ubiquitin-like Ubl proteins; Ubiquitin-like (Ubl) proteins have a similar ubiquitin (Ub) beta-grasp fold and attach to other proteins in a Ubl manner but with biochemically distinct roles. Ub and Ubl proteins conjugate and deconjugate via ligases and peptidases to covalently modify target polypeptides. Some Ubl domains have adaptor roles in Ub-signaling by mediating protein-protein interaction. Prokaryotic sulfur carrier proteins are Ub-related proteins that can be activated in an ATP-dependent manner. Polyubiquitination signals for a diverse set of cellular events via different isopeptide linkages formed between the C terminus of one ubiquitin (Ub) and the epsilon-amine of K6, K11, K27, K29, K33, K48, or K63 of a second Ub. One of these seven lysine residues (K27, Ub numbering) is conserved in this Ubl_ubiquitin_like family. K27-linked Ub chains are versatile and can be recognized by several downstream receptor proteins. K27 has roles beyond chain linkage, such as in Ubl NEDD8 (which contains many of the same lysines (K6, K11, K27, K33, K48) as Ub) where K27 has a role (other than conjugation) in the mechanism of protein neddylation.


Pssm-ID: 340559 [Multi-domain]  Cd Length: 68  Bit Score: 44.89  E-value: 2.40e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1812252035 346 VEVHFND-QVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDheapfgpEEMKySSRALHSFGIRDGDKIFV 419
Cdd:cd17039     1 ITVKTLDgKTYTVEVDPDDTVADLKEKIEEKTGIPVEQQRLIYNG-------KELK-DDKTLSDYGIKDGSTIHL 67
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 50-113 2.84e-05

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 45.16  E-value: 2.84e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1812252035  50 PSVLVLN--SCGITcagDEREIAAFCaHVSELDLSDNKLQDWHEVSKIVSNVPQLEFLNLSSNPLS 113
Cdd:cd21340   120 NSLRVLNisGNNID---SLEPLAPLR-NLEQLDASNNQISDLEELLDLLSSWPSLRELDLTGNPVC 181
Ubl_TBCB cd01789
ubiquitin-like (Ubl) domain found in tubulin-folding cofactor B (TBCB) and similar proteins; ...
 361-419 4.81e-04

ubiquitin-like (Ubl) domain found in tubulin-folding cofactor B (TBCB) and similar proteins; TBCB, also termed cytoskeleton-associated protein 1, or cytoskeleton-associated protein CKAPI, or tubulin-specific chaperone B, is one of protein cofactors A through E that is required for the folding of tubulins prior to their incorporation into microtubules and heterodimer assembly. TBCB comprises an N-terminal ubiquitin-like (Ubl) domain and a C-terminal cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain. The Ubl domain of TBCB is essential for proper folding and assembly of tubulin alpha. It has a beta-grasp Ubl fold, a common structure involved in protein-protein interactions. Ubiquitin (Ub) is a protein modifier in eukaryotes that is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. TBC-A through E are necessary for the biogenesis of microtubules and for cell viability.


Pssm-ID: 340487  Cd Length: 80  Bit Score: 38.70  E-value: 4.81e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1812252035 361 LDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 419
Cdd:cd01789    21 LSLTIGELKEKLELITGTPPSSMKLQLYDEDGKL-IGTLDDDDALLGSYPVRDGMRIHV 78
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 175-298 8.28e-04

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 40.54  E-value: 8.28e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812252035 175 SLKLLHITDNNLQDWTEIRKLgvmfPSLDTLVLANNHLNAIEEPADSlarlfpnlrsislhksglqswedidkLNSFPKL 254
Cdd:cd21340   121 SLRVLNISGNNIDSLEPLAPL----RNLEQLDASNNQISDLEELLDL--------------------------LSSWPSL 170

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1812252035 255 EEVRLLGIPLLQPYTTEERrklVVARLPSVSKLNGSVVTDGERE 298
Cdd:cd21340   171 RELDLTGNPVCKKPKYRDK---IILASKSLEVLDGKEITDTERQ 211
Ubiquitin_2 pfam14560
Ubiquitin-like domain; This entry contains ubiquitin-like domains.
 356-419 1.40e-03

Ubiquitin-like domain; This entry contains ubiquitin-like domains.


Pssm-ID: 405277  Cd Length: 83  Bit Score: 37.50  E-value: 1.40e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1812252035 356 EMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 419
Cdd:pfam14560  16 ERRFDKSLTIEELKEKLELITGTPPSSMRLQLYDDDDNL-VAKLDDDDALLGSYGVRDGMRIHV 78
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH