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Conserved domains on  [gi|2289990614|ref|NP_001398440|]
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isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial isoform 3 [Mus musculus]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
48-336 0e+00

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 536.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2289990614 286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLG 290
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 48-336 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 536.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2289990614 286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLG 290
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 34-336 2.51e-122

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 358.02  E-value: 2.51e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  34 APRRSISSPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKG 112
Cdd:PLN00123   14 AQRRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKG 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 113 NIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAK 192
Cdd:PLN00123   92 GLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFC 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:PLN00123  170 SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLY 249

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2289990614 273 GNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLK 336
Cdd:PLN00123  250 GNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQ 316
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 50-335 1.88e-106

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 316.95  E-value: 1.88e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  50 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 117
Cdd:COG0473     1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 118 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 185
Cdd:COG0473    77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 186 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:COG0473   155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2289990614 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHL 335
Cdd:COG0473   234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHL 303
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
52-334 3.21e-83

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 257.61  E-value: 3.21e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  52 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 123
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 124 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 192
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2289990614 273 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH 334
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRY 302
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 48-336 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 536.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2289990614 286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLG 290
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 34-336 2.51e-122

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 358.02  E-value: 2.51e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  34 APRRSISSPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKG 112
Cdd:PLN00123   14 AQRRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKG 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 113 NIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAK 192
Cdd:PLN00123   92 GLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFC 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:PLN00123  170 SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLY 249

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2289990614 273 GNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLK 336
Cdd:PLN00123  250 GNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQ 316
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 52-336 2.93e-114

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 337.62  E-value: 2.93e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  52 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSR 127
Cdd:PLN00118   43 TATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSL 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 128 NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQES 207
Cdd:PLN00118  121 NLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTH 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 208 GRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGP 287
Cdd:PLN00118  201 GRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGL 280

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*....
gi 2289990614 288 GLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:PLN00118  281 GLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLK 329
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 50-335 1.88e-106

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 316.95  E-value: 1.88e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  50 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 117
Cdd:COG0473     1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 118 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 185
Cdd:COG0473    77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 186 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:COG0473   155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2289990614 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHL 335
Cdd:COG0473   234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHL 303
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 52-335 1.49e-97

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 293.93  E-value: 1.49e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  52 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSR 127
Cdd:PRK08997    4 TITVIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 128 NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:PRK08997   82 NVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELAR 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:PRK08997  162 KEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVG 241

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 2289990614 286 GPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:PRK08997  242 GLGMAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYL 290
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
53-336 1.15e-96

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 296.42  E-value: 1.15e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SH 124
Cdd:PRK09222    7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGY 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 125 KSRNNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:PRK09222   82 KSLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEY 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 204 AQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 283
Cdd:PRK09222  162 ARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEI 241

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2289990614 284 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:PRK09222  242 SGSVGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIG 293
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
52-334 3.21e-83

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 257.61  E-value: 3.21e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  52 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 123
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 124 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 192
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2289990614 273 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH 334
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRY 302
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 51-336 5.72e-75

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 235.80  E-value: 5.72e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  51 HTVTMIPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppS 123
Cdd:PRK14025    2 HKICVIEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------T 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 124 HKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:PRK14025   73 AADVIVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEM 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 204 AQ----ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNV 279
Cdd:PRK14025  153 AKrrkkMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDG 232

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2289990614 280 CAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:PRK14025  233 AAGLVGGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLG 288
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 53-336 6.34e-75

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 240.05  E-value: 6.34e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSR 127
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 128 NNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 206
Cdd:TIGR02924  81 NVTLRKTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 207 SGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 286
Cdd:TIGR02924 161 HNRKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGS 240

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 2289990614 287 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLK 336
Cdd:TIGR02924 241 VGLAGSANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIG 289
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 53-335 6.37e-74

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 232.73  E-value: 6.37e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSH 124
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 125 KSRNNILRTSLDLYANVIHCKSLPGVVTRH-KDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:TIGR02088  75 KSVIVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 204 AQESGRKkVTAVHKANIMKLGDGLFLQCCREVAAHYpQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 283
Cdd:TIGR02088 154 AKERNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASAL 231

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2289990614 284 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:TIGR02088 232 AGSLGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYL 282
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 51-334 1.02e-48

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 168.36  E-value: 1.02e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  51 HTVTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NL 120
Cdd:PRK00772    3 YKIAVLPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 121 PPSHKSRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhKDIDILIVRENTEGEY-------SSLEHESVAgv 181
Cdd:PRK00772   83 PPDVRPERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA-- 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 182 VESLkIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQ 261
Cdd:PRK00772  158 FDTM-VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPK 234

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2289990614 262 QFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH 334
Cdd:PRK00772  235 QFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRY 307
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 55-335 1.31e-44

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 157.40  E-value: 1.31e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  55 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 116
Cdd:PRK03437    9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 117 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 185
Cdd:PRK03437   88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 186 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:PRK03437  156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2289990614 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:PRK03437  235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHL 306
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 52-335 6.10e-40

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 145.25  E-value: 6.10e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  52 TVTMIPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHN 119
Cdd:PRK08194    5 KIAVIPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 120 LPPSHKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHKDIDILIVRENTEGEYSSL-------EHEsvagVVESLK 186
Cdd:PRK08194   81 LVPDHISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNA 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 187 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccREVAAHYPQITFDSMIVDNTTMQLVSRPQQ 262
Cdd:PRK08194  157 VFTRKGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEE 231

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2289990614 263 FDVMVMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:PRK08194  232 FDVIVASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHF 306
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
56-332 7.47e-40

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 146.13  E-value: 7.47e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  56 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 124
Cdd:PRK06451   29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 125 KSRNNILRTSLDLYANVIHCKSLPGVVTRHKD---IDILIVRENTEGEYSSLEH----------------------ESVA 179
Cdd:PRK06451  107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 180 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVA---------------------AH 238
Cdd:PRK06451  187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVAlkefrdyvvteeevtknyngvPP 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 239 YPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 318
Cdd:PRK06451  265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343

                         330
                  ....*....|....
gi 2289990614 319 ANPTATLLASCMML 332
Cdd:PRK06451  344 ANPTGIIKGGELML 357
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 53-335 1.61e-39

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 143.70  E-value: 1.61e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  53 VTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPP 122
Cdd:TIGR00169   2 IAVLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 123 SHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------KDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAK 192
Cdd:TIGR00169  82 DQRPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPE 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 193 SLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:TIGR00169 162 IERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLF 239

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2289990614 273 GNIVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:TIGR00169 240 GDILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYS 302
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 56-335 8.52e-39

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 143.33  E-value: 8.52e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  56 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 122
Cdd:COG0538    24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 123 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 185
Cdd:COG0538    99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 186 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVA-AHYP-------------- 240
Cdd:COG0538   179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAeEEFGdkfitegpwekykg 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 241 -----QITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 310
Cdd:COG0538   259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335

                         330       340
                  ....*....|....*....|....*
gi 2289990614 311 ksIANKNIANPTATLLASCMMLDHL 335
Cdd:COG0538   336 --YAGKDSTNPGSLILSGTMMLRHR 358
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 102-335 1.36e-30

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 120.94  E-value: 1.36e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 102 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEH 175
Cdd:PRK07006   84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 176 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 230
Cdd:PRK07006  159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 231 CCREVA-----------AHYPQ---------ITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 290
Cdd:PRK07006  237 WGYQLAeeefgdelidgGPWDKiknpetgkeIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*
gi 2289990614 291 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHL 335
Cdd:PRK07006  317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHM 360
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 37-334 6.18e-25

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 105.15  E-value: 6.18e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614  37 RSISSPPSAKyggRHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRV 108
Cdd:PLN02329   36 RCAAASPGKK---RYNIALLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDA 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 109 ALKGNI------ETNHNLPPSHKSRNniLRTSLDLYANVIHCKSLPGVV---TRHKDI----DILIVRENTEGEYSSLE- 174
Cdd:PLN02329  113 ILLGAIggykwdKNEKHLRPEMALFY--LRRDLKVFANLRPATVLPQLVdasTLKKEVaegvDMMIVRELTGGIYFGEPr 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 175 ----HESVAGVVESLKIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAAHYPQITFDSMIVD 250
Cdd:PLN02329  191 gitiNENGEEVGVSTEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVD 268

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 251 NTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCM 330
Cdd:PLN02329  269 NAAMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAM 348


                  ....
gi 2289990614 331 MLDH 334
Cdd:PLN02329  349 LLKY 352
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
100-335 1.01e-20

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 93.24  E-value: 1.01e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 100 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSSL 173
Cdd:PRK07362   91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 174 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 223
Cdd:PRK07362  166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 224 GDGLFLQCCREVAA--------------------HYPQIT-----------FDSM------------------------- 247
Cdd:PRK07362  246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISiednarmiepgYDSLtpekkaaicaevkevldsiwsshgn 325

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2289990614 248 ------------IVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 315
Cdd:PRK07362  326 gkwkekvlvddrIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404

                         330       340
                  ....*....|....*....|
gi 2289990614 316 KNIANPTATLLASCMMLDHL 335
Cdd:PRK07362  405 LDRINPGSVILSGVMMLEYL 424
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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