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Conserved domains on  [gi|398365435|ref|NP_011574|]
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methylsterol monooxygenase [Saccharomyces cerevisiae S288C]

Protein Classification

sterol desaturase family protein( domain architecture ID 10513792)

sterol desaturase family protein is an oxidoreductase, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol

EC:  1.-.-.-
Gene Ontology:  GO:0006629|GO:0016491

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 147-282 1.08e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


:

Pssm-ID: 397991  Cd Length: 134  Bit Score: 91.34  E-value: 1.08e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  147 LFFVLEDTWHYWAHRLFH-YGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVmytgkLHLFTLCVWI 225
Cdd:pfam04116   3 LGLLLFDFLFYWVHRLLHrLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLG-----LPLLAFLLGL 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  226 TLRLFQAVDSHSG--YDFPWSLNKIMpfwAGAEHHDLHHHYF-IGNYASSFRWWDYCLDT 282
Cdd:pfam04116  78 LLGTLWYLFIHSGllFPLPWLLKRLL---GTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 147-282 1.08e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 91.34  E-value: 1.08e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  147 LFFVLEDTWHYWAHRLFH-YGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVmytgkLHLFTLCVWI 225
Cdd:pfam04116   3 LGLLLFDFLFYWVHRLLHrLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLG-----LPLLAFLLGL 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  226 TLRLFQAVDSHSG--YDFPWSLNKIMpfwAGAEHHDLHHHYF-IGNYASSFRWWDYCLDT 282
Cdd:pfam04116  78 LLGTLWYLFIHSGllFPLPWLLKRLL---GTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 147-278 2.24e-14

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 69.58  E-value: 2.24e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435 147 LFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAET-LSLGFGTVGMPILyvmytGkLHLFTLCVWI 225
Cdd:COG3000    4 LAFLLLDFVYYWLHRLSHRVPLLWRFHAVHHSSERMNLLTALRFHPLEIlLSALLFLLPLALL-----G-FPPEAVLLYL 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 398365435 226 TLRLFQAVDSHSGYDFPWS--LNKImpfWAGAEHHDLHHHYFI----GNYASSFRWWDY 278
Cdd:COG3000   78 ALNLLYQFFNHSNIRLPLDgpLEYV---FVTPSHHRVHHSRNPeyldKNYGGILSIWDR 133
PLN02869 PLN02869
fatty aldehyde decarbonylase
 156-282 9.95e-05

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 43.68  E-value: 9.95e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435 156 HYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAeYAHP-AETLSLgFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVD 234
Cdd:PLN02869 142 YYWLHRALHHHYLYSRYHSHHHSSIVTEPITS-VIHPfAEHIAY-FLLFAIPLLTTIFTGTASIAAFFGYISYIDFMNNM 219

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 398365435 235 SHSGYDF-PWSLNKIMPFWA----GAEHHDLHHHYFIGNYASSFRWWDYCLDT 282
Cdd:PLN02869 220 GHCNFELiPKWLFSIFPPLKylmyTPSYHSLHHTQFRTNYSLFMPIYDYIYGT 272
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 147-282 1.08e-22

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 91.34  E-value: 1.08e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  147 LFFVLEDTWHYWAHRLFH-YGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVmytgkLHLFTLCVWI 225
Cdd:pfam04116   3 LGLLLFDFLFYWVHRLLHrLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLG-----LPLLAFLLGL 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435  226 TLRLFQAVDSHSG--YDFPWSLNKIMpfwAGAEHHDLHHHYF-IGNYASSFRWWDYCLDT 282
Cdd:pfam04116  78 LLGTLWYLFIHSGllFPLPWLLKRLL---GTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 147-278 2.24e-14

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 69.58  E-value: 2.24e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435 147 LFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAET-LSLGFGTVGMPILyvmytGkLHLFTLCVWI 225
Cdd:COG3000    4 LAFLLLDFVYYWLHRLSHRVPLLWRFHAVHHSSERMNLLTALRFHPLEIlLSALLFLLPLALL-----G-FPPEAVLLYL 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 398365435 226 TLRLFQAVDSHSGYDFPWS--LNKImpfWAGAEHHDLHHHYFI----GNYASSFRWWDY 278
Cdd:COG3000   78 ALNLLYQFFNHSNIRLPLDgpLEYV---FVTPSHHRVHHSRNPeyldKNYGGILSIWDR 133
PLN02869 PLN02869
fatty aldehyde decarbonylase
 156-282 9.95e-05

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 43.68  E-value: 9.95e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398365435 156 HYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAeYAHP-AETLSLgFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVD 234
Cdd:PLN02869 142 YYWLHRALHHHYLYSRYHSHHHSSIVTEPITS-VIHPfAEHIAY-FLLFAIPLLTTIFTGTASIAAFFGYISYIDFMNNM 219

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 398365435 235 SHSGYDF-PWSLNKIMPFWA----GAEHHDLHHHYFIGNYASSFRWWDYCLDT 282
Cdd:PLN02869 220 GHCNFELiPKWLFSIFPPLKylmyTPSYHSLHHTQFRTNYSLFMPIYDYIYGT 272
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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