NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|6324038|ref|NP_014108|]
View 

Mid1p [Saccharomyces cerevisiae S288C]

Protein Classification

MID1 family protein( domain architecture ID 10584219)

MID1 family protein similar to Saccharomyces cerevisiae stretch-activated cation channel MID1 that is required for calcium influx and for vitality of MATa cells in a late, pheromone-induced event of the mating process requiring calcium induced signaling

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
Mid1 pfam12929
Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae ...
99-537 1.83e-169

Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae gene encoding a plasma membrane protein required for Ca2+ influx induced by the mating pheromone, alpha-factor. Mid1 protein plays a crucial role in supplying Ca2+ during the mating process. Mid1 is composed of 548-amino-acid residues with four hydrophobic regions named H1, H2, H3 and H4, and two cysteine-rich regions (C1 and C2) at the C-terminal. This family contains the H3, H4, C1 and C2 regions. suggesting that H1 is a signal sequence responsible for the alpha-factor-induced Mid1 delivery to the plasma membrane. The region from H1 to H3 is required for the localization of Mid1 in the plasma and ER membranes. Trafficking of Mid1-GFP to the plasma membrane is dependent on the N-glycosylation of Mid1 and the transporter protein Sec12. This findings suggests that the trafficking of Mid1-GFP to the plasma membrane requires a Sec12-dependent pathway from the ER to the Golgi, and that Mid1 is recruited via a Sec6- and Sec7-independent pathway from the Golgi to the plasma membrane.


:

Pssm-ID: 432882  Cd Length: 429  Bit Score: 486.74  E-value: 1.83e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038     99 LMFISGNICQMPM------NRSDVDLTIYYSFNETvLENPNIGQ------SAVFQDGYIQALaispvqssssnaTSTYSN 166
Cdd:pfam12929   1 TVYISANTCSQPQpdadntTSNPPQLTLYYSFSSS-NQNPGPGAdssgqtVVPFEEGYVMAL------------VNATGD 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    167 LYVVAELVNSTTeqplsssDASENWEYRLSISENDLVFQWDVRPWVEVLDTDMNSALLSTGNVT---ADAKVYHNYSIYD 243
Cdd:pfam12929  68 LYIGVSAPNSTT-------GFSGSWNYEIAASTDDLYHQYDDRPWLYLVDTDSNSALLVTGNLTtssADSTVYQSLMNLT 140
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    244 PSlYDLYVYSYEDSVQlnQNYNLSLCAVKNGPYLVSSQNTSNATVTSnstnplertdlaiqkkITEYG--GSVTEMFYVT 321
Cdd:pfam12929 141 PP-YTLFAYNTNDSSI--NGLNRSYCALQNGAQIASSSNSNSSVTTS----------------MTTRGlgNKPKQQFYVT 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    322 GLNASTTYVAYLTKKI-SNGDGLSSVGGILFSHVYFTTRSTDVCSLIFGLDFCSDVAYSVPTSSFSVGNKTLMAQTYDHI 400
Cdd:pfam12929 202 GLNSSTTYVGYLAKDGnSTASGVVGGGGKVFQPTNFTTKSDDNCALIFNLDFCDEVAYAVPSNPNKFPNKTELAKFYDDY 281
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    401 AEALYANFSKALQLISCDADKDARYSPVMTCDDCAEAYRDWVCAVSIPRCTTTSSQ--YYIHRDKS-------------H 465
Cdd:pfam12929 282 ARSLYANFSKALQQIPCDTESDARYSLARTCDDCARAYKNWLCAVTIPRCEDFSSTapYLQPRNVGqpfpngtsltatnS 361
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 6324038    466 NRNDYLNKFIKPlDDYYEILPCIDMCYTLVRNCPSDFQFSCPndlTTEDLLYQSYNFYMDTDYSTCNYIGNS 537
Cdd:pfam12929 362 SRNPFIDEVIKP-GPYKEVLPCIDLCYALVRSCPASLGFSCP---TPGSGLNLSYGWRDDDGDITCNYPGAA 429
 
Name Accession Description Interval E-value
Mid1 pfam12929
Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae ...
99-537 1.83e-169

Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae gene encoding a plasma membrane protein required for Ca2+ influx induced by the mating pheromone, alpha-factor. Mid1 protein plays a crucial role in supplying Ca2+ during the mating process. Mid1 is composed of 548-amino-acid residues with four hydrophobic regions named H1, H2, H3 and H4, and two cysteine-rich regions (C1 and C2) at the C-terminal. This family contains the H3, H4, C1 and C2 regions. suggesting that H1 is a signal sequence responsible for the alpha-factor-induced Mid1 delivery to the plasma membrane. The region from H1 to H3 is required for the localization of Mid1 in the plasma and ER membranes. Trafficking of Mid1-GFP to the plasma membrane is dependent on the N-glycosylation of Mid1 and the transporter protein Sec12. This findings suggests that the trafficking of Mid1-GFP to the plasma membrane requires a Sec12-dependent pathway from the ER to the Golgi, and that Mid1 is recruited via a Sec6- and Sec7-independent pathway from the Golgi to the plasma membrane.


Pssm-ID: 432882  Cd Length: 429  Bit Score: 486.74  E-value: 1.83e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038     99 LMFISGNICQMPM------NRSDVDLTIYYSFNETvLENPNIGQ------SAVFQDGYIQALaispvqssssnaTSTYSN 166
Cdd:pfam12929   1 TVYISANTCSQPQpdadntTSNPPQLTLYYSFSSS-NQNPGPGAdssgqtVVPFEEGYVMAL------------VNATGD 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    167 LYVVAELVNSTTeqplsssDASENWEYRLSISENDLVFQWDVRPWVEVLDTDMNSALLSTGNVT---ADAKVYHNYSIYD 243
Cdd:pfam12929  68 LYIGVSAPNSTT-------GFSGSWNYEIAASTDDLYHQYDDRPWLYLVDTDSNSALLVTGNLTtssADSTVYQSLMNLT 140
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    244 PSlYDLYVYSYEDSVQlnQNYNLSLCAVKNGPYLVSSQNTSNATVTSnstnplertdlaiqkkITEYG--GSVTEMFYVT 321
Cdd:pfam12929 141 PP-YTLFAYNTNDSSI--NGLNRSYCALQNGAQIASSSNSNSSVTTS----------------MTTRGlgNKPKQQFYVT 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    322 GLNASTTYVAYLTKKI-SNGDGLSSVGGILFSHVYFTTRSTDVCSLIFGLDFCSDVAYSVPTSSFSVGNKTLMAQTYDHI 400
Cdd:pfam12929 202 GLNSSTTYVGYLAKDGnSTASGVVGGGGKVFQPTNFTTKSDDNCALIFNLDFCDEVAYAVPSNPNKFPNKTELAKFYDDY 281
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    401 AEALYANFSKALQLISCDADKDARYSPVMTCDDCAEAYRDWVCAVSIPRCTTTSSQ--YYIHRDKS-------------H 465
Cdd:pfam12929 282 ARSLYANFSKALQQIPCDTESDARYSLARTCDDCARAYKNWLCAVTIPRCEDFSSTapYLQPRNVGqpfpngtsltatnS 361
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 6324038    466 NRNDYLNKFIKPlDDYYEILPCIDMCYTLVRNCPSDFQFSCPndlTTEDLLYQSYNFYMDTDYSTCNYIGNS 537
Cdd:pfam12929 362 SRNPFIDEVIKP-GPYKEVLPCIDLCYALVRSCPASLGFSCP---TPGSGLNLSYGWRDDDGDITCNYPGAA 429
 
Name Accession Description Interval E-value
Mid1 pfam12929
Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae ...
99-537 1.83e-169

Stretch-activated Ca2+-permeable channel component; MID1 is a yeast Saccharomyces cerevisiae gene encoding a plasma membrane protein required for Ca2+ influx induced by the mating pheromone, alpha-factor. Mid1 protein plays a crucial role in supplying Ca2+ during the mating process. Mid1 is composed of 548-amino-acid residues with four hydrophobic regions named H1, H2, H3 and H4, and two cysteine-rich regions (C1 and C2) at the C-terminal. This family contains the H3, H4, C1 and C2 regions. suggesting that H1 is a signal sequence responsible for the alpha-factor-induced Mid1 delivery to the plasma membrane. The region from H1 to H3 is required for the localization of Mid1 in the plasma and ER membranes. Trafficking of Mid1-GFP to the plasma membrane is dependent on the N-glycosylation of Mid1 and the transporter protein Sec12. This findings suggests that the trafficking of Mid1-GFP to the plasma membrane requires a Sec12-dependent pathway from the ER to the Golgi, and that Mid1 is recruited via a Sec6- and Sec7-independent pathway from the Golgi to the plasma membrane.


Pssm-ID: 432882  Cd Length: 429  Bit Score: 486.74  E-value: 1.83e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038     99 LMFISGNICQMPM------NRSDVDLTIYYSFNETvLENPNIGQ------SAVFQDGYIQALaispvqssssnaTSTYSN 166
Cdd:pfam12929   1 TVYISANTCSQPQpdadntTSNPPQLTLYYSFSSS-NQNPGPGAdssgqtVVPFEEGYVMAL------------VNATGD 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    167 LYVVAELVNSTTeqplsssDASENWEYRLSISENDLVFQWDVRPWVEVLDTDMNSALLSTGNVT---ADAKVYHNYSIYD 243
Cdd:pfam12929  68 LYIGVSAPNSTT-------GFSGSWNYEIAASTDDLYHQYDDRPWLYLVDTDSNSALLVTGNLTtssADSTVYQSLMNLT 140
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    244 PSlYDLYVYSYEDSVQlnQNYNLSLCAVKNGPYLVSSQNTSNATVTSnstnplertdlaiqkkITEYG--GSVTEMFYVT 321
Cdd:pfam12929 141 PP-YTLFAYNTNDSSI--NGLNRSYCALQNGAQIASSSNSNSSVTTS----------------MTTRGlgNKPKQQFYVT 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    322 GLNASTTYVAYLTKKI-SNGDGLSSVGGILFSHVYFTTRSTDVCSLIFGLDFCSDVAYSVPTSSFSVGNKTLMAQTYDHI 400
Cdd:pfam12929 202 GLNSSTTYVGYLAKDGnSTASGVVGGGGKVFQPTNFTTKSDDNCALIFNLDFCDEVAYAVPSNPNKFPNKTELAKFYDDY 281
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324038    401 AEALYANFSKALQLISCDADKDARYSPVMTCDDCAEAYRDWVCAVSIPRCTTTSSQ--YYIHRDKS-------------H 465
Cdd:pfam12929 282 ARSLYANFSKALQQIPCDTESDARYSLARTCDDCARAYKNWLCAVTIPRCEDFSSTapYLQPRNVGqpfpngtsltatnS 361
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 6324038    466 NRNDYLNKFIKPlDDYYEILPCIDMCYTLVRNCPSDFQFSCPndlTTEDLLYQSYNFYMDTDYSTCNYIGNS 537
Cdd:pfam12929 362 SRNPFIDEVIKP-GPYKEVLPCIDLCYALVRSCPASLGFSCP---TPGSGLNLSYGWRDDDGDITCNYPGAA 429
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH