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Conserved domains on  [gi|6324755|ref|NP_014824|]
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actin-binding protein LAS17 [Saccharomyces cerevisiae S288C]

Protein Classification

WASP family protein( domain architecture ID 10100405)

WASP (wiskott-Aldrich syndrome protein) family protein similar to Schizosaccharomyces pombe Wiskott-Aldrich syndrome protein homolog 1 (WSP1) that has a role in regulating actin assembly, so regulating polarized growth

Gene Ontology:  GO:0034315|GO:0003779|GO:0071933
PubMed:  9883880

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
 23-123 2.85e-46

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


:

Pssm-ID: 269916  Cd Length: 101  Bit Score: 158.46  E-value: 2.85e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755   23 IIDVTVARLYIAYPDKNEWQYTGLSGALALVDDLVGNTFFLKLVDINgHRGVIWDQELYVNFEYYQDRTFFHTFEMEECF 102
Cdd:cd01205   1 ILAAAVARLYYAEPDPDSWSYTGLTGALCFVKDNAKRSYFFRLVDLK-TRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|.
gi 6324755  103 AGLLFVDINEASHFLKRVQKR 123
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEK 100
 
Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
 23-123 2.85e-46

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 158.46  E-value: 2.85e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755   23 IIDVTVARLYIAYPDKNEWQYTGLSGALALVDDLVGNTFFLKLVDINgHRGVIWDQELYVNFEYYQDRTFFHTFEMEECF 102
Cdd:cd01205   1 ILAAAVARLYYAEPDPDSWSYTGLTGALCFVKDNAKRSYFFRLVDLK-TRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|.
gi 6324755  103 AGLLFVDINEASHFLKRVQKR 123
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEK 100
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
 13-124 4.97e-44

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 152.99  E-value: 4.97e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755     13 KRALPKASnKIIDVTVARLYIAYPDKNE-WQYTGLSGALALVDDLVGNTFFLKLVDINGHRgVIWDQELYVNFEYYQDRT 91
Cdd:pfam00568   1 FSALGKKC-QTICTAVAQVYLADPDNKRhWIKAKHSGVVCFVKDSPQNSYFIRLVDIQDGK-VIWNQEIYPNMEYNQARP 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 6324755     92 FFHTFEMEECFAGLLFVDINEASHFLKRVQKRE 124
Cdd:pfam00568  79 FFHTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
 17-124 2.83e-40

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 142.49  E-value: 2.83e-40
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755      17 PKASNKIIDVTVARLYIAYPDKneWQYTGLSGALALVDDLVGNTFFLKLVDINGHRGVIWDQELYVNFEYYQDRTFFHTF 96
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK--WVPTGEGGAANLVIDKNQRSYFFRIVGIKGQDKVIWNQELYKNFKYNQATPTFHQW 78

                           90       100
                   ....*....|....*....|....*...
gi 6324755      97 EMEECFAGLLFVDINEASHFLKRVQKRE 124
Cdd:smart00461  79 ADDKCVYGLNFASEEEAKKFRKKVLKAL 106
 
Name Accession Description Interval E-value
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
 23-123 2.85e-46

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 158.46  E-value: 2.85e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755   23 IIDVTVARLYIAYPDKNEWQYTGLSGALALVDDLVGNTFFLKLVDINgHRGVIWDQELYVNFEYYQDRTFFHTFEMEECF 102
Cdd:cd01205   1 ILAAAVARLYYAEPDPDSWSYTGLTGALCFVKDNAKRSYFFRLVDLK-TRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|.
gi 6324755  103 AGLLFVDINEASHFLKRVQKR 123
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEK 100
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
 13-124 4.97e-44

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 152.99  E-value: 4.97e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755     13 KRALPKASnKIIDVTVARLYIAYPDKNE-WQYTGLSGALALVDDLVGNTFFLKLVDINGHRgVIWDQELYVNFEYYQDRT 91
Cdd:pfam00568   1 FSALGKKC-QTICTAVAQVYLADPDNKRhWIKAKHSGVVCFVKDSPQNSYFIRLVDIQDGK-VIWNQEIYPNMEYNQARP 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 6324755     92 FFHTFEMEECFAGLLFVDINEASHFLKRVQKRE 124
Cdd:pfam00568  79 FFHTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
 17-124 2.83e-40

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 142.49  E-value: 2.83e-40
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755      17 PKASNKIIDVTVARLYIAYPDKneWQYTGLSGALALVDDLVGNTFFLKLVDINGHRGVIWDQELYVNFEYYQDRTFFHTF 96
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK--WVPTGEGGAANLVIDKNQRSYFFRIVGIKGQDKVIWNQELYKNFKYNQATPTFHQW 78

                           90       100
                   ....*....|....*....|....*...
gi 6324755      97 EMEECFAGLLFVDINEASHFLKRVQKRE 124
Cdd:smart00461  79 ADDKCVYGLNFASEEEAKKFRKKVLKAL 106
EVH1_family cd00837
EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; ...
 27-122 9.86e-09

EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; The EVH1 domains are part of the PH domain superfamily. EVH1 subfamilies include Enables/VASP, Homer/Vesl, WASP, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269909  Cd Length: 103  Bit Score: 53.23  E-value: 9.86e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324755   27 TVARLYIAYPDKNEWQYTGLSGA--LALVDDLVGNTFFLKLVDINGHRgVIWDQELYVNFEYYQDRTFFHTFEMEECFAG 104
Cdd:cd00837   5 ARAHVMQIDDSNKNWVPAGGKGAsrVSYFKDTTRNSFRIIGVDIKDKK-VVINCTITKNLVYNKATQTFHQWADDRTVFG 83

                        90
                ....*....|....*...
gi 6324755  105 LLFVDINEASHFLKRVQK 122
Cdd:cd00837  84 LNFASEEDATKFAEAVQE 101
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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