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Conserved domains on  [gi|6325129|ref|NP_015197|]
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Tbf1p [Saccharomyces cerevisiae S288C]

Protein Classification

telomere repeat binding factor family protein( domain architecture ID 10554101)

telomere repeat binding factor (TRF) family protein binds directly to the telomeric double-stranded TTAGGG repeat through its SANT (SWI3, ADA2, N-CoR and TFIIIB)/Myb-like DNA-binding domain, and negatively regulates telomere length

CATH:  1.20.58.1880
Gene Ontology:  GO:0042162|GO:0010833|GO:0006355|GO:0003700|GO:0003677|GO:0000723
PubMed:  8882580|12757977|15608118|22244753
SCOP:  4000317

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TRF pfam08558
Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are ...
 32-283 5.12e-65

Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are important for the regulation of telomere stability. The two related human TRF proteins hTRF1 and hTRF2 form homodimers and bind directly to telomeric TTAGGG repeats via the myb DNA binding domain pfam00249 at the carboxy terminus. TRF1 is implicated in telomere length regulation and TRF2 in telomere protection. Other telomere complex associated proteins are recruited through their interaction with either TRF1 or TRF2. The fission yeast protein Taz1p (telomere-associated in Schizosaccharomyces pombe) has similarity to both hTRF1 and hTRF2 and may perform the dual functions of TRF1 and TRF2 at fission yeast telomeres. This domain is composed of multiple alpha helices arranged in a solenoid conformation similar to TPR repeats. The fungal members have now also been found to carry two double strand telomeric repeat binding factors.


:

Pssm-ID: 400735  Cd Length: 212  Bit Score: 211.11  E-value: 5.12e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129     32 SLCLLDNISTQLLRFLILNANSpNIIAVLTDQtaflSSGETEIFQTLVKLFKQIRMIYHTRSPLLSvhdvapglwfpnsp 111
Cdd:pfam08558   1 SLWVLDNLSTQLLRALREGPYT-DVLAIVSEP----DSEFRESFDILESLFERTKKLYSTRSPLLS-------------- 61

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    112 pPLILRGHEAFIITAIRKANLLTFLLTSLNCLNYGFELLQSIFLDIFCPNTntvgnnsleqsGKFLKSQAILYLDLKTQA 191
Cdd:pfam08558  62 -PSILKIRESYIMQTLRRINLGTFLDSVFGNLEVGFLELAENFLDIFCPED-----------GKLLKPQGVLYLDLKTQA 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    192 YIAGLKefqdetnEISLEKKQELLDLIFPSNLADILVQRrtgdsgDITLLTPSEKDFVERCDRRRENLKIVQD----FNS 267
Cdd:pfam08558 130 YIVALK-------EGPFKSASEILDKLFPDDLADILKQR------NDKLLTPSETDFVERCKKRREKLLNIINekdpYHP 196

                         250
                  ....*....|....*.
gi 6325129    268 LTQSYEWAQFIRELLD 283
Cdd:pfam08558 197 LLQNFSWESFLKELLS 212
SANT_TRF cd11660
Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human ...
 410-462 1.73e-12

Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human telomere repeat binding factors, TRF1 and TRF2, function as part of the 6 component shelterin complex. TRF2 binds DNA and recruits RAP1 (via binding to the RAP1 protein c-terminal (RCT)) and TIN2 in the protection of telomeres from DNA repair machinery. Metazoan shelterin consists of 3 DNA binding proteins (TRF2, TRF1, and POT1) and 3 recruited proteins that bind to one or more of these DNA-binding proteins (RAP1, TIN2, TPP1). Schizosaccharomyces pombe TAZ1 is an orthlog and binds RAP1. Human TRF1 and TRF2 bind double-stranded DNA. hTRF2 consists of a basic N-terminus, a TRF homology domain, the RAP1 binding motif (RBM), the TIN2 binding motif (TBM) and a myb-like DNA binding domain, SANT, named after 'SWI3, ADA2, N-CoR and TFIIIB', several factors that share this domain. Tandem copies of the domain bind telomeric DNA tandem repeats as part of the capping complex. The single myb-like domain of TRF-type proteins is similar to the tandem myb_like domains found in yeast RAP1.


:

Pssm-ID: 212558 [Multi-domain]  Cd Length: 50  Bit Score: 61.81  E-value: 1.73e-12
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 6325129  410 RTWSKEEEEALVEGLKEVGP-SWSKILDLYGPggkitenLKNRTQVQLKDKARN 462
Cdd:cd11660   1 RKWTDEEDEALVEGVEKYGVgNWAKILKDYFF-------VNNRTSVDLKDKWRN 47
 
Name Accession Description Interval E-value
TRF pfam08558
Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are ...
 32-283 5.12e-65

Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are important for the regulation of telomere stability. The two related human TRF proteins hTRF1 and hTRF2 form homodimers and bind directly to telomeric TTAGGG repeats via the myb DNA binding domain pfam00249 at the carboxy terminus. TRF1 is implicated in telomere length regulation and TRF2 in telomere protection. Other telomere complex associated proteins are recruited through their interaction with either TRF1 or TRF2. The fission yeast protein Taz1p (telomere-associated in Schizosaccharomyces pombe) has similarity to both hTRF1 and hTRF2 and may perform the dual functions of TRF1 and TRF2 at fission yeast telomeres. This domain is composed of multiple alpha helices arranged in a solenoid conformation similar to TPR repeats. The fungal members have now also been found to carry two double strand telomeric repeat binding factors.


Pssm-ID: 400735  Cd Length: 212  Bit Score: 211.11  E-value: 5.12e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129     32 SLCLLDNISTQLLRFLILNANSpNIIAVLTDQtaflSSGETEIFQTLVKLFKQIRMIYHTRSPLLSvhdvapglwfpnsp 111
Cdd:pfam08558   1 SLWVLDNLSTQLLRALREGPYT-DVLAIVSEP----DSEFRESFDILESLFERTKKLYSTRSPLLS-------------- 61

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    112 pPLILRGHEAFIITAIRKANLLTFLLTSLNCLNYGFELLQSIFLDIFCPNTntvgnnsleqsGKFLKSQAILYLDLKTQA 191
Cdd:pfam08558  62 -PSILKIRESYIMQTLRRINLGTFLDSVFGNLEVGFLELAENFLDIFCPED-----------GKLLKPQGVLYLDLKTQA 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    192 YIAGLKefqdetnEISLEKKQELLDLIFPSNLADILVQRrtgdsgDITLLTPSEKDFVERCDRRRENLKIVQD----FNS 267
Cdd:pfam08558 130 YIVALK-------EGPFKSASEILDKLFPDDLADILKQR------NDKLLTPSETDFVERCKKRREKLLNIINekdpYHP 196

                         250
                  ....*....|....*.
gi 6325129    268 LTQSYEWAQFIRELLD 283
Cdd:pfam08558 197 LLQNFSWESFLKELLS 212
SANT_TRF cd11660
Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human ...
 410-462 1.73e-12

Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human telomere repeat binding factors, TRF1 and TRF2, function as part of the 6 component shelterin complex. TRF2 binds DNA and recruits RAP1 (via binding to the RAP1 protein c-terminal (RCT)) and TIN2 in the protection of telomeres from DNA repair machinery. Metazoan shelterin consists of 3 DNA binding proteins (TRF2, TRF1, and POT1) and 3 recruited proteins that bind to one or more of these DNA-binding proteins (RAP1, TIN2, TPP1). Schizosaccharomyces pombe TAZ1 is an orthlog and binds RAP1. Human TRF1 and TRF2 bind double-stranded DNA. hTRF2 consists of a basic N-terminus, a TRF homology domain, the RAP1 binding motif (RBM), the TIN2 binding motif (TBM) and a myb-like DNA binding domain, SANT, named after 'SWI3, ADA2, N-CoR and TFIIIB', several factors that share this domain. Tandem copies of the domain bind telomeric DNA tandem repeats as part of the capping complex. The single myb-like domain of TRF-type proteins is similar to the tandem myb_like domains found in yeast RAP1.


Pssm-ID: 212558 [Multi-domain]  Cd Length: 50  Bit Score: 61.81  E-value: 1.73e-12
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 6325129  410 RTWSKEEEEALVEGLKEVGP-SWSKILDLYGPggkitenLKNRTQVQLKDKARN 462
Cdd:cd11660   1 RKWTDEEDEALVEGVEKYGVgNWAKILKDYFF-------VNNRTSVDLKDKWRN 47
Myb_DNA-binding pfam00249
Myb-like DNA-binding domain; This family contains the DNA binding domains from Myb proteins, ...
 409-464 7.17e-08

Myb-like DNA-binding domain; This family contains the DNA binding domains from Myb proteins, as well as the SANT domain family.


Pssm-ID: 459731 [Multi-domain]  Cd Length: 46  Bit Score: 48.65  E-value: 7.17e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 6325129    409 KRTWSKEEEEALVEGLKEVGPSWSKILDLYGpggkitenlkNRTQVQLKDKARNWK 464
Cdd:pfam00249   1 RGPWTPEEDELLLEAVEKLGNRWKKIAKLLP----------GRTDNQCKNRWQNYL 46
SANT smart00717
SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains;
409-464 1.13e-07

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains;


Pssm-ID: 197842 [Multi-domain]  Cd Length: 49  Bit Score: 48.37  E-value: 1.13e-07
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 6325129     409 KRTWSKEEEEALVEGLKEVGP-SWSKILdlygpggkitENLKNRTQVQLKDKARNWK 464
Cdd:smart00717   1 KGEWTEEEDELLIELVKKYGKnNWEKIA----------KELPGRTAEQCRERWRNLL 47
 
Name Accession Description Interval E-value
TRF pfam08558
Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are ...
 32-283 5.12e-65

Telomere repeat binding factor (TRF); Telomere repeat binding factor (TRF) family proteins are important for the regulation of telomere stability. The two related human TRF proteins hTRF1 and hTRF2 form homodimers and bind directly to telomeric TTAGGG repeats via the myb DNA binding domain pfam00249 at the carboxy terminus. TRF1 is implicated in telomere length regulation and TRF2 in telomere protection. Other telomere complex associated proteins are recruited through their interaction with either TRF1 or TRF2. The fission yeast protein Taz1p (telomere-associated in Schizosaccharomyces pombe) has similarity to both hTRF1 and hTRF2 and may perform the dual functions of TRF1 and TRF2 at fission yeast telomeres. This domain is composed of multiple alpha helices arranged in a solenoid conformation similar to TPR repeats. The fungal members have now also been found to carry two double strand telomeric repeat binding factors.


Pssm-ID: 400735  Cd Length: 212  Bit Score: 211.11  E-value: 5.12e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129     32 SLCLLDNISTQLLRFLILNANSpNIIAVLTDQtaflSSGETEIFQTLVKLFKQIRMIYHTRSPLLSvhdvapglwfpnsp 111
Cdd:pfam08558   1 SLWVLDNLSTQLLRALREGPYT-DVLAIVSEP----DSEFRESFDILESLFERTKKLYSTRSPLLS-------------- 61

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    112 pPLILRGHEAFIITAIRKANLLTFLLTSLNCLNYGFELLQSIFLDIFCPNTntvgnnsleqsGKFLKSQAILYLDLKTQA 191
Cdd:pfam08558  62 -PSILKIRESYIMQTLRRINLGTFLDSVFGNLEVGFLELAENFLDIFCPED-----------GKLLKPQGVLYLDLKTQA 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325129    192 YIAGLKefqdetnEISLEKKQELLDLIFPSNLADILVQRrtgdsgDITLLTPSEKDFVERCDRRRENLKIVQD----FNS 267
Cdd:pfam08558 130 YIVALK-------EGPFKSASEILDKLFPDDLADILKQR------NDKLLTPSETDFVERCKKRREKLLNIINekdpYHP 196

                         250
                  ....*....|....*.
gi 6325129    268 LTQSYEWAQFIRELLD 283
Cdd:pfam08558 197 LLQNFSWESFLKELLS 212
SANT_TRF cd11660
Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human ...
 410-462 1.73e-12

Telomere repeat binding factor-like DNA-binding domains of the SANT/myb-like family; Human telomere repeat binding factors, TRF1 and TRF2, function as part of the 6 component shelterin complex. TRF2 binds DNA and recruits RAP1 (via binding to the RAP1 protein c-terminal (RCT)) and TIN2 in the protection of telomeres from DNA repair machinery. Metazoan shelterin consists of 3 DNA binding proteins (TRF2, TRF1, and POT1) and 3 recruited proteins that bind to one or more of these DNA-binding proteins (RAP1, TIN2, TPP1). Schizosaccharomyces pombe TAZ1 is an orthlog and binds RAP1. Human TRF1 and TRF2 bind double-stranded DNA. hTRF2 consists of a basic N-terminus, a TRF homology domain, the RAP1 binding motif (RBM), the TIN2 binding motif (TBM) and a myb-like DNA binding domain, SANT, named after 'SWI3, ADA2, N-CoR and TFIIIB', several factors that share this domain. Tandem copies of the domain bind telomeric DNA tandem repeats as part of the capping complex. The single myb-like domain of TRF-type proteins is similar to the tandem myb_like domains found in yeast RAP1.


Pssm-ID: 212558 [Multi-domain]  Cd Length: 50  Bit Score: 61.81  E-value: 1.73e-12
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 6325129  410 RTWSKEEEEALVEGLKEVGP-SWSKILDLYGPggkitenLKNRTQVQLKDKARN 462
Cdd:cd11660   1 RKWTDEEDEALVEGVEKYGVgNWAKILKDYFF-------VNNRTSVDLKDKWRN 47
Myb_DNA-binding pfam00249
Myb-like DNA-binding domain; This family contains the DNA binding domains from Myb proteins, ...
 409-464 7.17e-08

Myb-like DNA-binding domain; This family contains the DNA binding domains from Myb proteins, as well as the SANT domain family.


Pssm-ID: 459731 [Multi-domain]  Cd Length: 46  Bit Score: 48.65  E-value: 7.17e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 6325129    409 KRTWSKEEEEALVEGLKEVGPSWSKILDLYGpggkitenlkNRTQVQLKDKARNWK 464
Cdd:pfam00249   1 RGPWTPEEDELLLEAVEKLGNRWKKIAKLLP----------GRTDNQCKNRWQNYL 46
SANT smart00717
SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains;
409-464 1.13e-07

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains;


Pssm-ID: 197842 [Multi-domain]  Cd Length: 49  Bit Score: 48.37  E-value: 1.13e-07
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 6325129     409 KRTWSKEEEEALVEGLKEVGP-SWSKILdlygpggkitENLKNRTQVQLKDKARNWK 464
Cdd:smart00717   1 KGEWTEEEDELLIELVKKYGKnNWEKIA----------KELPGRTAEQCRERWRNLL 47
SANT cd00167
'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric ...
 411-464 6.87e-07

'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is also found in regulatory transcriptional repressor complexes where it also binds DNA.


Pssm-ID: 238096 [Multi-domain]  Cd Length: 45  Bit Score: 46.03  E-value: 6.87e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 6325129  411 TWSKEEEEALVEGLKEVGP-SWSKILdlygpggkitENLKNRTQVQLKDKARNWK 464
Cdd:cd00167   1 PWTEEEDELLLEAVKKYGKnNWEKIA----------KELPGRTPKQCRERWRNLL 45
SANT_DMAP1_like cd11658
SANT/myb-like domain of Human Dna Methyltransferase 1 Associated Protein 1-like; These ...
 412-459 1.67e-03

SANT/myb-like domain of Human Dna Methyltransferase 1 Associated Protein 1-like; These proteins are members of the SANT/myb group. SANT is named after 'SWI3, ADA2, N-CoR and TFIIIB', several factors that share this domain. The SANT domain resembles the 3 alpha-helix bundle of the DNA-binding Myb domains and is found in a diverse set of proteins.


Pssm-ID: 212556  Cd Length: 46  Bit Score: 36.62  E-value: 1.67e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*...
gi 6325129  412 WSKEEEEALVEGLKEVGPSWSKILDLYGPGgkitenlKNRTQVQLKDK 459
Cdd:cd11658   1 WTKEETDYLFDLVKRFDLRWNVILDRYPFQ-------KGRSVEDLKEK 41
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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