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Conserved domains on  [gi|15597898|ref|NP_251392|]
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hypothetical protein PA2702 [Pseudomonas aeruginosa PAO1]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ADPRTs_Tse2 super family cl39967
Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ...
 7-152 2.05e-75

Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ADP-ribosylating toxins. It is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa and found mostly in gamma proteobacteria. It naturally attacks a target in the cytoplasm of bacterial cells. Structural analysis shows similarity between Tse2 and nicotinamide adenine dinucleotide (NAD)-dependent enzymes from bacteria, notably the mono-ADP-ribosyltransferase toxins (ADPRTs). Furthermore, it revealed that the Tse2 active site is occluded upon binding the cognate immunity protein Tsi2. The abrogation of toxicity for the R14A, S80A, and H122A mutant Tse2 proteins indicates the importance of these amino acids in the mechanism of Tse2 toxicity and, given their conservation with NAD-reactive enzymes, also supports their assignment as being involved in a catalytic reaction.


The actual alignment was detected with superfamily member pfam18648:

Pssm-ID: 408430  Cd Length: 155  Bit Score: 222.06  E-value: 2.05e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597898     7 KTSLTLYRAVFKANYDGDVGRYLHPDKELAEAAEVAPLLHPTFDSPNTPGVPARaPDIVAGRDGLYAPD----TGGTSVF 82
Cdd:pfam18648   1 KTSTDLYRAIKKGNSGKDVSVYFHPDGEAADGLLYARFEHTTYSGADGEGRVSR-ADIMLERDGLDRPDyvltDGGTSVF 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15597898    83 DRAGVLRRADGDFV-IPDGTDIPPDLKVKQD-----SYNKRLQATHYTIMPAKPMYREVLMGQLDNFVRNAIRRQW 152
Cdd:pfam18648  80 DVPGWFGRDDWDYFsIPKGTDYPEGLKIKRDakkkwNRNKTLSATHYTIEPKKPMTVDAYKGALDNFARAAIARQV 155
 
Name Accession Description Interval E-value
ADPRTs_Tse2 pfam18648
Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ...
 7-152 2.05e-75

Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ADP-ribosylating toxins. It is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa and found mostly in gamma proteobacteria. It naturally attacks a target in the cytoplasm of bacterial cells. Structural analysis shows similarity between Tse2 and nicotinamide adenine dinucleotide (NAD)-dependent enzymes from bacteria, notably the mono-ADP-ribosyltransferase toxins (ADPRTs). Furthermore, it revealed that the Tse2 active site is occluded upon binding the cognate immunity protein Tsi2. The abrogation of toxicity for the R14A, S80A, and H122A mutant Tse2 proteins indicates the importance of these amino acids in the mechanism of Tse2 toxicity and, given their conservation with NAD-reactive enzymes, also supports their assignment as being involved in a catalytic reaction.


Pssm-ID: 408430  Cd Length: 155  Bit Score: 222.06  E-value: 2.05e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597898     7 KTSLTLYRAVFKANYDGDVGRYLHPDKELAEAAEVAPLLHPTFDSPNTPGVPARaPDIVAGRDGLYAPD----TGGTSVF 82
Cdd:pfam18648   1 KTSTDLYRAIKKGNSGKDVSVYFHPDGEAADGLLYARFEHTTYSGADGEGRVSR-ADIMLERDGLDRPDyvltDGGTSVF 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15597898    83 DRAGVLRRADGDFV-IPDGTDIPPDLKVKQD-----SYNKRLQATHYTIMPAKPMYREVLMGQLDNFVRNAIRRQW 152
Cdd:pfam18648  80 DVPGWFGRDDWDYFsIPKGTDYPEGLKIKRDakkkwNRNKTLSATHYTIEPKKPMTVDAYKGALDNFARAAIARQV 155
 
Name Accession Description Interval E-value
ADPRTs_Tse2 pfam18648
Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ...
 7-152 2.05e-75

Tse2 ADP-ribosyltransferase toxins; Tse2 from P. aeruginosa has structural features similar to ADP-ribosylating toxins. It is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa and found mostly in gamma proteobacteria. It naturally attacks a target in the cytoplasm of bacterial cells. Structural analysis shows similarity between Tse2 and nicotinamide adenine dinucleotide (NAD)-dependent enzymes from bacteria, notably the mono-ADP-ribosyltransferase toxins (ADPRTs). Furthermore, it revealed that the Tse2 active site is occluded upon binding the cognate immunity protein Tsi2. The abrogation of toxicity for the R14A, S80A, and H122A mutant Tse2 proteins indicates the importance of these amino acids in the mechanism of Tse2 toxicity and, given their conservation with NAD-reactive enzymes, also supports their assignment as being involved in a catalytic reaction.


Pssm-ID: 408430  Cd Length: 155  Bit Score: 222.06  E-value: 2.05e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597898     7 KTSLTLYRAVFKANYDGDVGRYLHPDKELAEAAEVAPLLHPTFDSPNTPGVPARaPDIVAGRDGLYAPD----TGGTSVF 82
Cdd:pfam18648   1 KTSTDLYRAIKKGNSGKDVSVYFHPDGEAADGLLYARFEHTTYSGADGEGRVSR-ADIMLERDGLDRPDyvltDGGTSVF 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15597898    83 DRAGVLRRADGDFV-IPDGTDIPPDLKVKQD-----SYNKRLQATHYTIMPAKPMYREVLMGQLDNFVRNAIRRQW 152
Cdd:pfam18648  80 DVPGWFGRDDWDYFsIPKGTDYPEGLKIKRDakkkwNRNKTLSATHYTIEPKKPMTVDAYKGALDNFARAAIARQV 155
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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