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Conserved domains on  [gi|15599452|ref|NP_252946|]
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50S ribosomal protein L16 [Pseudomonas aeruginosa PAO1]

Protein Classification

large ribosomal subunit protein uL16( domain architecture ID 10785085)

large ribosomal subunit protein uL16, also called 50S ribosomal protein L16 in bacteria, binds 23S rRNA and appears to make contacts with the A and possibly P site tRNAs

Gene Symbol:  rpl16
Gene Ontology:  GO:0019843|GO:0003735|GO:0005840|GO:0000049|GO:0006412
PubMed:  14527328|24524803|12900959
SCOP:  4003717

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RplP COG0197
Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal ...
 1-113 1.23e-68

Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal protein L16/L10AE is part of the Pathway/BioSystem: Ribosome 50S subunit


:

Pssm-ID: 439967  Cd Length: 136  Bit Score: 202.96  E-value: 1.23e-68
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452   1 MLQPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLE 80
Cdd:COG0197   1 MLMPKRVKYRKQQRGRIKGVAKRGNKFAFGNYGLKALEPGQITSRQIEAARIAITRYLKRGGKVWIRIFPDKPLTKKPAE 80

                        90       100       110
                ....*....|....*....|....*....|...
gi 15599452  81 VRMGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:COG0197  81 TRMGAGKGKPEGWVAVVKPGRILFEIAGVSEEV 113
 
Name Accession Description Interval E-value
RplP COG0197
Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal ...
 1-113 1.23e-68

Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal protein L16/L10AE is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 439967  Cd Length: 136  Bit Score: 202.96  E-value: 1.23e-68
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452   1 MLQPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLE 80
Cdd:COG0197   1 MLMPKRVKYRKQQRGRIKGVAKRGNKFAFGNYGLKALEPGQITSRQIEAARIAITRYLKRGGKVWIRIFPDKPLTKKPAE 80

                        90       100       110
                ....*....|....*....|....*....|...
gi 15599452  81 VRMGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:COG0197  81 TRMGAGKGKPEGWVAVVKPGRILFEIAGVSEEV 113
rplP_bact TIGR01164
ribosomal protein L16, bacterial/organelle; This model describes bacterial and organellar ...
 3-113 8.50e-63

ribosomal protein L16, bacterial/organelle; This model describes bacterial and organellar ribosomal protein L16. The homologous protein of the eukaryotic cytosol is designated L10 [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273476  Cd Length: 125  Bit Score: 187.97  E-value: 8.50e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452     3 QPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLEVR 82
Cdd:TIGR01164   1 SPKRTKYRKQQRGRVKGKAKRGNTVAFGEYGLQALEPGWITARQIEAARVAMTRYVKRGGKLWIRIFPDKPYTKKPLETR 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 15599452    83 MGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:TIGR01164  81 MGKGKGNPEYWVAVVKPGKILFEIAGVPEEV 111
rpl16 CHL00044
ribosomal protein L16
 1-113 9.88e-60

ribosomal protein L16


Pssm-ID: 176985  Cd Length: 135  Bit Score: 180.48  E-value: 9.88e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452    1 MLQPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLE 80
Cdd:CHL00044   1 MLSPKRTKFRKQHRGRMKGISYRGNRICFGRYALQALEPAWITSRQIEAGRRAITRYARRGGKIWIRIFPDKPVTMRPAE 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 15599452   81 VRMGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:CHL00044  81 TRMGSGKGSPEYWVAVVKPGRILYEMGGVSETI 113
Ribosomal_L16 pfam00252
Ribosomal protein L16p/L10e;
4-113 3.18e-52

Ribosomal protein L16p/L10e;


Pssm-ID: 459733  Cd Length: 132  Bit Score: 161.44  E-value: 3.18e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452     4 PKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVK--RGGKIWIRVFPDKPVTKKPLEV 81
Cdd:pfam00252   1 PKRTKYRKSRKRYIRGVPTKGTTLDFGDYGLVALEGGQITARQLEAARIAINRYLKktRGGKLWIRVFPDKPVTKKPGED 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 15599452    82 RMGKGKGGVEYWVAQIQPGKVLYEI-EGVSEEL 113
Cdd:pfam00252  81 RMGKGKGKPEGWVARVKPGRILFEIkGGVHEEV 113
Ribosomal_L16_L10e cd01433
Ribosomal_L16_L10e: L16 is an essential protein in the large ribosomal subunit of bacteria, ...
 23-112 3.45e-42

Ribosomal_L16_L10e: L16 is an essential protein in the large ribosomal subunit of bacteria, mitochondria, and chloroplasts. Large subunits that lack L16 are defective in peptidyl transferase activity, peptidyl-tRNA hydrolysis activity, association with the 30S subunit, binding of aminoacyl-tRNA and interaction with antibiotics. L16 is required for the function of elongation factor P (EF-P), a protein involved in peptide bond synthesis through the stimulation of peptidyl transferase activity by the ribosome. Mutations in L16 and the adjoining bases of 23S rRNA confer antibiotic resistance in bacteria, suggesting a role for L16 in the formation of the antibiotic binding site. The GTPase RbgA (YlqF) is essential for the assembly of the large subunit, and it is believed to regulate the incorporation of L16. L10e is the archaeal and eukaryotic cytosolic homolog of bacterial L16. L16 and L10e exhibit structural differences at the N-terminus.


Pssm-ID: 238714  Cd Length: 112  Bit Score: 135.31  E-value: 3.45e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452  23 RGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKR-GGKIWIRVFPDKPVTKKPLEVRMGKGKGGVEYWVAQIQPGK 101
Cdd:cd01433   1 KGNKLFFGEYGLVALEPGRITAEQLEAARIAINRYLKKkGGKLWIRVFPDHPVTKKPLETRMGKGKGKPEGWVARVKPGQ 80

                        90
                ....*....|.
gi 15599452 102 VLYEIEGVSEE 112
Cdd:cd01433  81 ILFEVRGVPEE 91
 
Name Accession Description Interval E-value
RplP COG0197
Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal ...
 1-113 1.23e-68

Ribosomal protein L16/L10AE [Translation, ribosomal structure and biogenesis]; Ribosomal protein L16/L10AE is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 439967  Cd Length: 136  Bit Score: 202.96  E-value: 1.23e-68
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452   1 MLQPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLE 80
Cdd:COG0197   1 MLMPKRVKYRKQQRGRIKGVAKRGNKFAFGNYGLKALEPGQITSRQIEAARIAITRYLKRGGKVWIRIFPDKPLTKKPAE 80

                        90       100       110
                ....*....|....*....|....*....|...
gi 15599452  81 VRMGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:COG0197  81 TRMGAGKGKPEGWVAVVKPGRILFEIAGVSEEV 113
rplP_bact TIGR01164
ribosomal protein L16, bacterial/organelle; This model describes bacterial and organellar ...
 3-113 8.50e-63

ribosomal protein L16, bacterial/organelle; This model describes bacterial and organellar ribosomal protein L16. The homologous protein of the eukaryotic cytosol is designated L10 [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273476  Cd Length: 125  Bit Score: 187.97  E-value: 8.50e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452     3 QPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLEVR 82
Cdd:TIGR01164   1 SPKRTKYRKQQRGRVKGKAKRGNTVAFGEYGLQALEPGWITARQIEAARVAMTRYVKRGGKLWIRIFPDKPYTKKPLETR 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 15599452    83 MGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:TIGR01164  81 MGKGKGNPEYWVAVVKPGKILFEIAGVPEEV 111
rpl16 CHL00044
ribosomal protein L16
 1-113 9.88e-60

ribosomal protein L16


Pssm-ID: 176985  Cd Length: 135  Bit Score: 180.48  E-value: 9.88e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452    1 MLQPKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPVTKKPLE 80
Cdd:CHL00044   1 MLSPKRTKFRKQHRGRMKGISYRGNRICFGRYALQALEPAWITSRQIEAGRRAITRYARRGGKIWIRIFPDKPVTMRPAE 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 15599452   81 VRMGKGKGGVEYWVAQIQPGKVLYEIEGVSEEL 113
Cdd:CHL00044  81 TRMGSGKGSPEYWVAVVKPGRILYEMGGVSETI 113
Ribosomal_L16 pfam00252
Ribosomal protein L16p/L10e;
4-113 3.18e-52

Ribosomal protein L16p/L10e;


Pssm-ID: 459733  Cd Length: 132  Bit Score: 161.44  E-value: 3.18e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452     4 PKRTKFRKQMTGHNRGLAHRGSKVSFGEYALKATSRGRLTARQIESARRALTRHVK--RGGKIWIRVFPDKPVTKKPLEV 81
Cdd:pfam00252   1 PKRTKYRKSRKRYIRGVPTKGTTLDFGDYGLVALEGGQITARQLEAARIAINRYLKktRGGKLWIRVFPDKPVTKKPGED 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 15599452    82 RMGKGKGGVEYWVAQIQPGKVLYEI-EGVSEEL 113
Cdd:pfam00252  81 RMGKGKGKPEGWVARVKPGRILFEIkGGVHEEV 113
Ribosomal_L16_L10e cd01433
Ribosomal_L16_L10e: L16 is an essential protein in the large ribosomal subunit of bacteria, ...
 23-112 3.45e-42

Ribosomal_L16_L10e: L16 is an essential protein in the large ribosomal subunit of bacteria, mitochondria, and chloroplasts. Large subunits that lack L16 are defective in peptidyl transferase activity, peptidyl-tRNA hydrolysis activity, association with the 30S subunit, binding of aminoacyl-tRNA and interaction with antibiotics. L16 is required for the function of elongation factor P (EF-P), a protein involved in peptide bond synthesis through the stimulation of peptidyl transferase activity by the ribosome. Mutations in L16 and the adjoining bases of 23S rRNA confer antibiotic resistance in bacteria, suggesting a role for L16 in the formation of the antibiotic binding site. The GTPase RbgA (YlqF) is essential for the assembly of the large subunit, and it is believed to regulate the incorporation of L16. L10e is the archaeal and eukaryotic cytosolic homolog of bacterial L16. L16 and L10e exhibit structural differences at the N-terminus.


Pssm-ID: 238714  Cd Length: 112  Bit Score: 135.31  E-value: 3.45e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599452  23 RGSKVSFGEYALKATSRGRLTARQIESARRALTRHVKR-GGKIWIRVFPDKPVTKKPLEVRMGKGKGGVEYWVAQIQPGK 101
Cdd:cd01433   1 KGNKLFFGEYGLVALEPGRITAEQLEAARIAINRYLKKkGGKLWIRVFPDHPVTKKPLETRMGKGKGKPEGWVARVKPGQ 80

                        90
                ....*....|.
gi 15599452 102 VLYEIEGVSEE 112
Cdd:cd01433  81 ILFEVRGVPEE 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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