NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|15832353|ref|NP_311126|]
View 

malate dehydrogenase [Escherichia coli O157:H7 str. Sakai]

Protein Classification

malate:quinone oxidoreductase( domain architecture ID 10012287)

malate:quinone oxidoreductase catalyzes the oxidation of malate to oxaloacetate using quinone as the electron acceptor as part of the citric acid cycle

Gene Ontology:  GO:0008924

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
PRK05257 PRK05257
malate:quinone oxidoreductase; Validated
27-520 0e+00

malate:quinone oxidoreductase; Validated


:

Pssm-ID: 179979  Cd Length: 494  Bit Score: 1043.56  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   27 SEEQETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAV 106
Cdd:PRK05257   1 SEESKTDVVLIGGGIMSATLGTLLKELEPEWSITMFERLDGVALESSNGWNNAGTGHSALCELNYTPEKADGSIDISKAV 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  107 AINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVM 186
Cdd:PRK05257  81 KINEQFQISRQFWAYLVEKGVLPDPRSFINPVPHMSFVWGEDNVAFLKKRYEALKANPLFAGMEFSEDPAQIKEWAPLMM 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  187 EGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFI 266
Cdd:PRK05257 161 EGRDPSQKVAATRIEIGTDVNFGALTRQLVGYLQKQGNFELQLGHEVRDIKRNDDGSWTVTVKDLKTGEKRTVRAKFVFI 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  267 GAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFAT 346
Cdd:PRK05257 241 GAGGGALPLLQKSGIPEAKGYGGFPVSGQFLVCENPEVVAQHHAKVYGKASVGAPPMSVPHLDTRVIDGKRSLLFGPFAG 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  347 FSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIK 426
Cdd:PRK05257 321 FSTKFLKNGSLLDLFSSVRPSNLLPMLAVGLDNFDLTKYLISQVMLSDEDRFEALREFYPNAKPEDWELIVAGQRVQIIK 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  427 RDADKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATE 506
Cdd:PRK05257 401 KDPKKGGVLQFGTEVVSSADGSIAALLGASPGASTAVPIMLEVLEKCFPDRMASPEWQPKLKEMIPSYGQKLNDNPALLE 480
                        490
                 ....*....|....
gi 15832353  507 RELQYTSEVLGLKY 520
Cdd:PRK05257 481 EIRAYTSEVLGLDY 494
 
Name Accession Description Interval E-value
PRK05257 PRK05257
malate:quinone oxidoreductase; Validated
27-520 0e+00

malate:quinone oxidoreductase; Validated


Pssm-ID: 179979  Cd Length: 494  Bit Score: 1043.56  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   27 SEEQETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAV 106
Cdd:PRK05257   1 SEESKTDVVLIGGGIMSATLGTLLKELEPEWSITMFERLDGVALESSNGWNNAGTGHSALCELNYTPEKADGSIDISKAV 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  107 AINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVM 186
Cdd:PRK05257  81 KINEQFQISRQFWAYLVEKGVLPDPRSFINPVPHMSFVWGEDNVAFLKKRYEALKANPLFAGMEFSEDPAQIKEWAPLMM 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  187 EGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFI 266
Cdd:PRK05257 161 EGRDPSQKVAATRIEIGTDVNFGALTRQLVGYLQKQGNFELQLGHEVRDIKRNDDGSWTVTVKDLKTGEKRTVRAKFVFI 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  267 GAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFAT 346
Cdd:PRK05257 241 GAGGGALPLLQKSGIPEAKGYGGFPVSGQFLVCENPEVVAQHHAKVYGKASVGAPPMSVPHLDTRVIDGKRSLLFGPFAG 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  347 FSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIK 426
Cdd:PRK05257 321 FSTKFLKNGSLLDLFSSVRPSNLLPMLAVGLDNFDLTKYLISQVMLSDEDRFEALREFYPNAKPEDWELIVAGQRVQIIK 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  427 RDADKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATE 506
Cdd:PRK05257 401 KDPKKGGVLQFGTEVVSSADGSIAALLGASPGASTAVPIMLEVLEKCFPDRMASPEWQPKLKEMIPSYGQKLNDNPALLE 480
                        490
                 ....*....|....
gi 15832353  507 RELQYTSEVLGLKY 520
Cdd:PRK05257 481 EIRAYTSEVLGLDY 494
Mqo pfam06039
Malate:quinone oxidoreductase (Mqo); This family consists of several bacterial Malate:quinone ...
31-518 0e+00

Malate:quinone oxidoreductase (Mqo); This family consists of several bacterial Malate:quinone oxidoreductase (Mqo) proteins (EC:1.1.99.16). Mqo takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. The enzyme is therefore called malate:quinone oxidoreductase, abbreviated to Mqo. Mqo is a peripheral membrane protein and can be released from the membrane by addition of chelators.


Pssm-ID: 461809  Cd Length: 488  Bit Score: 1014.67  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353    31 ETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINE 110
Cdd:pfam06039   1 KVDVVLIGAGIMSATLGTLLKELEPDWSITVFERLDAVAAESSNAWNNAGTGHSALCELNYTPEKPDGSIDISKAVKINE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   111 AFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVMEGRD 190
Cdd:pfam06039  81 QFEVSRQFWAYLVENGVLPDPKSFINPVPHMSFVWGEDNVAFLRKRYEALSAHPLFEGMEFSEDPAQIAEWAPLMMEGRD 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   191 PQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGG 270
Cdd:pfam06039 161 PSEPVAATRIELGTDVNFGALTRQLVAYLQKQPGVELHYGHEVTDLKRNSDGRWRVKVKDLNTGEKRTVRAKFVFIGAGG 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   271 AALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTK 350
Cdd:pfam06039 241 GALPLLQKSGIPEAKGYGGFPVSGQFLRCTNPEVIAQHHAKVYGKAAVGAPPMSVPHLDTRVIDGKKSLLFGPFAGFSTK 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   351 FLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAD 430
Cdd:pfam06039 321 FLKNGSLLDLFKSIRPSNILPMLAVGLDNLDLTKYLIGQVLQSHEDRMEALREFFPEAKDEDWELITAGQRVQVIKKDEE 400
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   431 KGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERELQ 510
Cdd:pfam06039 401 KGGVLQFGTEVVASADGSIAALLGASPGASTAVSIMLEVLERCFPERMASPEWQPKLKEMIPSYGQKLNDDPELLAEIRA 480

                  ....*...
gi 15832353   511 YTSEVLGL 518
Cdd:pfam06039 481 RTSKVLGL 488
mal_quin_oxido TIGR01320
malate:quinone-oxidoreductase; This membrane-associated enzyme is an alternative to the ...
32-516 0e+00

malate:quinone-oxidoreductase; This membrane-associated enzyme is an alternative to the better-known NAD-dependent malate dehydrogenase as part of the TCA cycle. The reduction of a quinone rather than NAD+ makes the reaction essentially irreversible in the direction of malate oxidation to oxaloacetate. Both forms of malate dehydrogenase are active in E. coli; disruption of this form causes less phenotypic change. In some bacteria, this form is the only or the more important malate dehydrogenase. [Energy metabolism, TCA cycle]


Pssm-ID: 130387  Cd Length: 483  Bit Score: 683.87  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353    32 TDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINEA 111
Cdd:TIGR01320   1 TDVVLIGAGIMSATLGVLLRELEPNWSITLIERLDAVAAESSNPWNNAGTGHSALCELNYTPEMADGSIDIAKAVGINEQ 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   112 FQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVMEGRDP 191
Cdd:TIGR01320  81 FQVSRQFWAHLVEEGILTDPKSFINPVPHVSFVHGSDGVAYLKKRYEALKGHPLFEGMEFSEDPATFAEWLPLMAAGRDF 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   192 QQKVAATRTEIGTDVNYGEITRQLIASLQKKSNfSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGA 271
Cdd:TIGR01320 161 SEPVAANWAAEGTDVDFGALTKQLLGYLVQNGT-TIRFGHEVRNLKRQSDGSWTVTVKNTRTGGKRTLNTRFVFVGAGGG 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   272 ALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKF 351
Cdd:TIGR01320 240 ALPLLQKSGIPEVKGFAGFPVSGLFLRCGNPELTEQHRAKVYGQASVGAPPMSVPHLDTRVVDGKKWLLFGPYAGWSPKF 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   352 LKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADK 431
Cdd:TIGR01320 320 LKHGSILDLPLSIRPDNLLSMLGVGLTEMDLTKYLIGQLRKSEEERVSALREFYPEAIDSDWELIVAGQRVQVIKKDPEK 399
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   432 G-GVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRvsSPQWQATLKAIVPSYGRKLNGDVAATERELQ 510
Cdd:TIGR01320 400 GgGVLEFGTTLIADADGSIAGLLGASPGASTAVSIMLDLLERCFPER--YQSWLPKLKEMVPSYGKKLSSEPALFDEVWS 477

                  ....*.
gi 15832353   511 YTSEVL 516
Cdd:TIGR01320 478 RTTKVL 483
LhgO COG0579
L-2-hydroxyglutarate oxidase LhgO [Carbohydrate transport and metabolism];
28-506 3.95e-154

L-2-hydroxyglutarate oxidase LhgO [Carbohydrate transport and metabolism];


Pssm-ID: 440344 [Multi-domain]  Cd Length: 418  Bit Score: 447.29  E-value: 3.95e-154
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  28 EEQETDVLLIGGGIMSATLGTYLRELEpEWSMTMVERLEGVAQESSNgwNNAGTGHSALmelNYTPqnadGSISIEKAVA 107
Cdd:COG0579   1 MMEMYDVVIIGAGIVGLALARELSRYE-DLKVLVLEKEDDVAQESSG--NNSGVIHAGL---YYTP----GSLKARLCVE 70
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 108 INEAF-QISRQfwaHQVErgvlrtprsfINTVPHMSFVWGEDNVNFLRARYAALQQSsLFRGMRYSeDHAQIKEWAPLVM 186
Cdd:COG0579  71 GNELFyELCRE---LGIP----------FKRCGKLVVATGEEEVAFLEKLYERGKAN-GVPGLEIL-DREELRELEPLLS 135
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 187 EgrdpqQKVAATRTEIGTDVNYGEITRQLIASLQKKsNFSLQLSSEVRALKRNDDNtWTVTVAdlkNGTaqnIRAKFVFI 266
Cdd:COG0579 136 D-----EGVAALYSPSTGIVDPGALTRALAENAEAN-GVELLLNTEVTGIEREGDG-WEVTTN---GGT---IRARFVIN 202
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 267 GAGGAALKLLQESGIpeAKDYAGFPVGGQFLVSENP-DVVNHhlaKVYGKASVGAPPMSVpHIdTRVLDGKrvVLFGPFA 345
Cdd:COG0579 203 AAGLYADRLAQMAGI--GKDFGIFPVKGEYLVLDKPaELVNA---KVYPVPDPGAPFLGV-HL-TRTIDGN--LLFGPNA 273
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 346 TFSTKflKNGSLWDLMSSTTTSNVMPMMHvglDNFdLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQII 425
Cdd:COG0579 274 VFVPK--KEDSLLDLFESLRFPNFWPMLA---KNL-LTKYLESVTSLSKEAFLEALRKYVPELPDEDLIPAFAGIRAQII 347
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 426 KRDadkggvlrlGTEVV--SDQQGTIAALLGASPGASTAAPIMLNLLEKvFGDRVSSPQWQATLKAIvpSYGrKLNGDVA 503
Cdd:COG0579 348 KPD---------GDFVIeeADDPGSIHVLGIESPGATSALAIAEHVAEL-LPEKLEEKDWQPKRKMI--SFG-ELKEDPA 414

                ...
gi 15832353 504 ATE 506
Cdd:COG0579 415 YGE 417
 
Name Accession Description Interval E-value
PRK05257 PRK05257
malate:quinone oxidoreductase; Validated
27-520 0e+00

malate:quinone oxidoreductase; Validated


Pssm-ID: 179979  Cd Length: 494  Bit Score: 1043.56  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   27 SEEQETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAV 106
Cdd:PRK05257   1 SEESKTDVVLIGGGIMSATLGTLLKELEPEWSITMFERLDGVALESSNGWNNAGTGHSALCELNYTPEKADGSIDISKAV 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  107 AINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVM 186
Cdd:PRK05257  81 KINEQFQISRQFWAYLVEKGVLPDPRSFINPVPHMSFVWGEDNVAFLKKRYEALKANPLFAGMEFSEDPAQIKEWAPLMM 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  187 EGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFI 266
Cdd:PRK05257 161 EGRDPSQKVAATRIEIGTDVNFGALTRQLVGYLQKQGNFELQLGHEVRDIKRNDDGSWTVTVKDLKTGEKRTVRAKFVFI 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  267 GAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFAT 346
Cdd:PRK05257 241 GAGGGALPLLQKSGIPEAKGYGGFPVSGQFLVCENPEVVAQHHAKVYGKASVGAPPMSVPHLDTRVIDGKRSLLFGPFAG 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  347 FSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIK 426
Cdd:PRK05257 321 FSTKFLKNGSLLDLFSSVRPSNLLPMLAVGLDNFDLTKYLISQVMLSDEDRFEALREFYPNAKPEDWELIVAGQRVQIIK 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  427 RDADKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATE 506
Cdd:PRK05257 401 KDPKKGGVLQFGTEVVSSADGSIAALLGASPGASTAVPIMLEVLEKCFPDRMASPEWQPKLKEMIPSYGQKLNDNPALLE 480
                        490
                 ....*....|....
gi 15832353  507 RELQYTSEVLGLKY 520
Cdd:PRK05257 481 EIRAYTSEVLGLDY 494
Mqo pfam06039
Malate:quinone oxidoreductase (Mqo); This family consists of several bacterial Malate:quinone ...
31-518 0e+00

Malate:quinone oxidoreductase (Mqo); This family consists of several bacterial Malate:quinone oxidoreductase (Mqo) proteins (EC:1.1.99.16). Mqo takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. The enzyme is therefore called malate:quinone oxidoreductase, abbreviated to Mqo. Mqo is a peripheral membrane protein and can be released from the membrane by addition of chelators.


Pssm-ID: 461809  Cd Length: 488  Bit Score: 1014.67  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353    31 ETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINE 110
Cdd:pfam06039   1 KVDVVLIGAGIMSATLGTLLKELEPDWSITVFERLDAVAAESSNAWNNAGTGHSALCELNYTPEKPDGSIDISKAVKINE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   111 AFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVMEGRD 190
Cdd:pfam06039  81 QFEVSRQFWAYLVENGVLPDPKSFINPVPHMSFVWGEDNVAFLRKRYEALSAHPLFEGMEFSEDPAQIAEWAPLMMEGRD 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   191 PQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGG 270
Cdd:pfam06039 161 PSEPVAATRIELGTDVNFGALTRQLVAYLQKQPGVELHYGHEVTDLKRNSDGRWRVKVKDLNTGEKRTVRAKFVFIGAGG 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   271 AALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTK 350
Cdd:pfam06039 241 GALPLLQKSGIPEAKGYGGFPVSGQFLRCTNPEVIAQHHAKVYGKAAVGAPPMSVPHLDTRVIDGKKSLLFGPFAGFSTK 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   351 FLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAD 430
Cdd:pfam06039 321 FLKNGSLLDLFKSIRPSNILPMLAVGLDNLDLTKYLIGQVLQSHEDRMEALREFFPEAKDEDWELITAGQRVQVIKKDEE 400
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   431 KGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERELQ 510
Cdd:pfam06039 401 KGGVLQFGTEVVASADGSIAALLGASPGASTAVSIMLEVLERCFPERMASPEWQPKLKEMIPSYGQKLNDDPELLAEIRA 480

                  ....*...
gi 15832353   511 YTSEVLGL 518
Cdd:pfam06039 481 RTSKVLGL 488
PRK13339 PRK13339
malate:quinone oxidoreductase; Reviewed
26-520 0e+00

malate:quinone oxidoreductase; Reviewed


Pssm-ID: 183983  Cd Length: 497  Bit Score: 706.84  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   26 ASEEQETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKA 105
Cdd:PRK13339   1 MAKSESKDVVLVGAGILSTTFGVLLKELDPDWNIEVVERLDSPAIESSNEWNNAGTGHAALCELNYTVQQPDGSIDIEKA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  106 VAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLV 185
Cdd:PRK13339  81 KEINEQFEISKQFWGHLVKSGTIGNPREFINPLPHISFVRGKNNVKFLKKRYEALKQHPMFDNIEYTEDIEVMAKWMPLM 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  186 MEGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVF 265
Cdd:PRK13339 161 MPGREANEIMAASKIDEGTDVNFGALTRKLAKHLESHPNAQVKYNHEVVDLERLSDGGWEVTVKDRNTGEKREQVADYVF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  266 IGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFA 345
Cdd:PRK13339 241 IGAGGGAIPLLQKSGIPESKHLGGFPISGQFLRCTNPEVVKQHQAKVYSKEPVGTPPMTVPHLDTRYIDGKRSLLFGPYA 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  346 TFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQII 425
Cdd:PRK13339 321 GFGPKFLKHGSNLDLFKSVKPYNITTMLAVAVKNMPLIKYSIDQVMQTKEGRMNHLRTFYPEARAEDWRLYTAGKRVQVI 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  426 KRDADKG-GVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSspQWQATLKAIVPSYGRKLNGDVAA 504
Cdd:PRK13339 401 KDTPEHGkGFIQFGTEVVNSQDHSVIALLGESPGASTSVSVALEVLERNFPEYAK--EWAPKLKEMIPSYGKSLIEDAKL 478
                        490
                 ....*....|....*.
gi 15832353  505 TERELQYTSEVLGLKY 520
Cdd:PRK13339 479 MRKIRKQTSKDLELGY 494
mal_quin_oxido TIGR01320
malate:quinone-oxidoreductase; This membrane-associated enzyme is an alternative to the ...
32-516 0e+00

malate:quinone-oxidoreductase; This membrane-associated enzyme is an alternative to the better-known NAD-dependent malate dehydrogenase as part of the TCA cycle. The reduction of a quinone rather than NAD+ makes the reaction essentially irreversible in the direction of malate oxidation to oxaloacetate. Both forms of malate dehydrogenase are active in E. coli; disruption of this form causes less phenotypic change. In some bacteria, this form is the only or the more important malate dehydrogenase. [Energy metabolism, TCA cycle]


Pssm-ID: 130387  Cd Length: 483  Bit Score: 683.87  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353    32 TDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINEA 111
Cdd:TIGR01320   1 TDVVLIGAGIMSATLGVLLRELEPNWSITLIERLDAVAAESSNPWNNAGTGHSALCELNYTPEMADGSIDIAKAVGINEQ 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   112 FQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKEWAPLVMEGRDP 191
Cdd:TIGR01320  81 FQVSRQFWAHLVEEGILTDPKSFINPVPHVSFVHGSDGVAYLKKRYEALKGHPLFEGMEFSEDPATFAEWLPLMAAGRDF 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   192 QQKVAATRTEIGTDVNYGEITRQLIASLQKKSNfSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGA 271
Cdd:TIGR01320 161 SEPVAANWAAEGTDVDFGALTKQLLGYLVQNGT-TIRFGHEVRNLKRQSDGSWTVTVKNTRTGGKRTLNTRFVFVGAGGG 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   272 ALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKF 351
Cdd:TIGR01320 240 ALPLLQKSGIPEVKGFAGFPVSGLFLRCGNPELTEQHRAKVYGQASVGAPPMSVPHLDTRVVDGKKWLLFGPYAGWSPKF 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   352 LKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADK 431
Cdd:TIGR01320 320 LKHGSILDLPLSIRPDNLLSMLGVGLTEMDLTKYLIGQLRKSEEERVSALREFYPEAIDSDWELIVAGQRVQVIKKDPEK 399
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   432 G-GVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRvsSPQWQATLKAIVPSYGRKLNGDVAATERELQ 510
Cdd:TIGR01320 400 GgGVLEFGTTLIADADGSIAGLLGASPGASTAVSIMLDLLERCFPER--YQSWLPKLKEMVPSYGKKLSSEPALFDEVWS 477

                  ....*.
gi 15832353   511 YTSEVL 516
Cdd:TIGR01320 478 RTTKVL 483
LhgO COG0579
L-2-hydroxyglutarate oxidase LhgO [Carbohydrate transport and metabolism];
28-506 3.95e-154

L-2-hydroxyglutarate oxidase LhgO [Carbohydrate transport and metabolism];


Pssm-ID: 440344 [Multi-domain]  Cd Length: 418  Bit Score: 447.29  E-value: 3.95e-154
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  28 EEQETDVLLIGGGIMSATLGTYLRELEpEWSMTMVERLEGVAQESSNgwNNAGTGHSALmelNYTPqnadGSISIEKAVA 107
Cdd:COG0579   1 MMEMYDVVIIGAGIVGLALARELSRYE-DLKVLVLEKEDDVAQESSG--NNSGVIHAGL---YYTP----GSLKARLCVE 70
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 108 INEAF-QISRQfwaHQVErgvlrtprsfINTVPHMSFVWGEDNVNFLRARYAALQQSsLFRGMRYSeDHAQIKEWAPLVM 186
Cdd:COG0579  71 GNELFyELCRE---LGIP----------FKRCGKLVVATGEEEVAFLEKLYERGKAN-GVPGLEIL-DREELRELEPLLS 135
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 187 EgrdpqQKVAATRTEIGTDVNYGEITRQLIASLQKKsNFSLQLSSEVRALKRNDDNtWTVTVAdlkNGTaqnIRAKFVFI 266
Cdd:COG0579 136 D-----EGVAALYSPSTGIVDPGALTRALAENAEAN-GVELLLNTEVTGIEREGDG-WEVTTN---GGT---IRARFVIN 202
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 267 GAGGAALKLLQESGIpeAKDYAGFPVGGQFLVSENP-DVVNHhlaKVYGKASVGAPPMSVpHIdTRVLDGKrvVLFGPFA 345
Cdd:COG0579 203 AAGLYADRLAQMAGI--GKDFGIFPVKGEYLVLDKPaELVNA---KVYPVPDPGAPFLGV-HL-TRTIDGN--LLFGPNA 273
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 346 TFSTKflKNGSLWDLMSSTTTSNVMPMMHvglDNFdLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQII 425
Cdd:COG0579 274 VFVPK--KEDSLLDLFESLRFPNFWPMLA---KNL-LTKYLESVTSLSKEAFLEALRKYVPELPDEDLIPAFAGIRAQII 347
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 426 KRDadkggvlrlGTEVV--SDQQGTIAALLGASPGASTAAPIMLNLLEKvFGDRVSSPQWQATLKAIvpSYGrKLNGDVA 503
Cdd:COG0579 348 KPD---------GDFVIeeADDPGSIHVLGIESPGATSALAIAEHVAEL-LPEKLEEKDWQPKRKMI--SFG-ELKEDPA 414

                ...
gi 15832353 504 ATE 506
Cdd:COG0579 415 YGE 417
PTZ00383 PTZ00383
malate:quinone oxidoreductase; Provisional
33-461 1.24e-20

malate:quinone oxidoreductase; Provisional


Pssm-ID: 240393 [Multi-domain]  Cd Length: 497  Bit Score: 95.19  E-value: 1.24e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   33 DVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPqnadgsisiEKAVAINEAF 112
Cdd:PTZ00383  47 DVVIVGGGVTGTALFYTLSKFTNLKKIALIERRSDFALVASHGKNNSQTIHCGDIETNYTL---------EKARKVKRQA 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  113 QISRQFWAH--QVERgvlrtpRSFINTVPHMSFVWGEDNVNFLRARYAALQQssLFRGMRYSEDHaQIKEWAPLVMEGRD 190
Cdd:PTZ00383 118 DMLRNYLTKlpPSER------DSIIFKMQKMVLGVGEKECEFLEKRYPVFKE--LFPSMQLLDKK-EIHRVEPRVVLKNN 188
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  191 ----PQQKVAATRTEIGTDVNYGEITRQLIASLQKKSN-----FSLQLSSEVRALKRNDDNTWTVTVadlKNGTaqnIRA 261
Cdd:PTZ00383 189 htlrEEPLAALYVPNELTTVDYQKLSESFVKHARRDALvpgkkISINLNTEVLNIERSNDSLYKIHT---NRGE---IRA 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  262 KFVFIGAGGAALKLLQESGIpeAKDYAGFPVGGQFLVSenPDVVNhhlAKVYgkaSVGAP--PMSVPHIDTR-VLDGKrv 338
Cdd:PTZ00383 263 RFVVVSACGYSLLFAQKMGY--GLEYSCLPVAGSFYFS--GNILN---GKVY---TVQNPalPFAAVHGDPDiIAKGK-- 330
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  339 VLFGPFATFSTKFLKN--GSLWDLMSsttTSNVMP-MMHVGLDNF---DLVKYLVSQVM-----LSEEDRFEALKEYYPQ 407
Cdd:PTZ00383 331 TRFGPTALPLPLLERYnmSSLPDFLK---VWNPDLnLLAVYFDLFkdsTMRKYVLRNFLfevplLNKYLFLKDARKIVPS 407
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 15832353  408 AKKEDWRLWQA--GQRVQIIKRDADKggvLRLGTEVVSDQQGTIAALLgASPGAST 461
Cdd:PTZ00383 408 LTRKDLRYCVGygGVRPQLIDKVSKK---LLLGEGKIDPGKGIIFNIT-PSPGATT 459
DadA COG0665
Glycine/D-amino acid oxidase (deaminating) [Amino acid transport and metabolism];
30-303 3.62e-08

Glycine/D-amino acid oxidase (deaminating) [Amino acid transport and metabolism];


Pssm-ID: 440429 [Multi-domain]  Cd Length: 364  Bit Score: 55.68  E-value: 3.62e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  30 QETDVLLIGGGIMSATLGTYLRELepEWSMTMVERlEGVAQESSngWNNAGtghsalmelNYTPQNADgsISIEKAVAIN 109
Cdd:COG0665   1 ATADVVVIGGGIAGLSTAYHLARR--GLDVTVLER-GRPGSGAS--GRNAG---------QLRPGLAA--LADRALVRLA 64
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 110 EAfqiSRQFWAHQVERGVLRTPrsfINTVPHMSFVWGEDNVNFLRARYAALQQsslfRGMRYSE-DHAQIKEWAPLVmeg 188
Cdd:COG0665  65 RE---ALDLWRELAAELGIDCD---FRRTGVLYLARTEAELAALRAEAEALRA----LGLPVELlDAAELREREPGL--- 131
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353 189 rDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKsNFSLQLSSEVRALKRNDDNTWTVTVADlknGTaqnIRAKFVFIGA 268
Cdd:COG0665 132 -GSPDYAGGLYDPDDGHVDPAKLVRALARAARAA-GVRIREGTPVTGLEREGGRVTGVRTER---GT---VRADAVVLAA 203
                       250       260       270
                ....*....|....*....|....*....|....*
gi 15832353 269 GGAALKLLQESGIpeakDYAGFPVGGQFLVSENPD 303
Cdd:COG0665 204 GAWSARLLPMLGL----RLPLRPVRGYVLVTEPLP 234
mnmC PRK01747
bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent ...
24-295 1.04e-04

bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC;


Pssm-ID: 234978 [Multi-domain]  Cd Length: 662  Bit Score: 45.22  E-value: 1.04e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353   24 AKASEEQETDVLLIGGGIMSATlgTYLRELEPEWSMTMVERLEGVAQESSNgwNNAGtghsALMelnytPQ-NADGSISI 102
Cdd:PRK01747 253 ARPGSPKARDAAIIGGGIAGAA--LALALARRGWQVTLYEADEAPAQGASG--NRQG----ALY-----PLlSKDDNALS 319
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  103 EKAVAineAFQISRQFWAHQVERGVlRTPRSFiNTVPHmsFVWGEDNVNFLRARYAALQQSSLFRGMrysedhaqikewa 182
Cdd:PRK01747 320 RFFRA---AFLFARRFYDALPAAGV-AFDHDW-CGVLQ--LAWDEKSAEKIAKMLALGLPAELARAL------------- 379
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832353  183 plvmegrDPQQkvaaTRTEIGTDVNYG-------------EITRQLIASLQKKsnFSLQLSSEVRALKRNDDnTWTvtva 249
Cdd:PRK01747 380 -------DAEE----AEELAGLPVPCGgifypqggwlcpaELCRALLALAGQQ--LTIHFGHEVARLEREDD-GWQ---- 441
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 15832353  250 dLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPeakdyaGFPVGGQ 295
Cdd:PRK01747 442 -LDFAGGTLASAPVVVLANGHDAARFAQTAHLP------LYSVRGQ 480
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH