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Conserved domains on  [gi|15832937|ref|NP_311710|]
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prepilin peptidase-dependent protein A [Escherichia coli O157:H7 str. Sakai]

Protein Classification

prepilin peptidase-dependent protein( domain architecture ID 11484750)

prepilin peptidase-dependent protein is a type IV pilin and may be involved in adhesion, DNA uptake, phage transduction, aggregation and a flagella-independent movement called twitching motility

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10506 PRK10506
prepilin peptidase-dependent protein;
1-156 8.68e-85

prepilin peptidase-dependent protein;


:

Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 245.67  E-value: 8.68e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832937    1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISFIREGTLWCLVSS 80
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSAWGLYGWQRWQQRQRLWQTAQQLLDFLLRLQEDANWHNRDHILWLIREGQGWCLVSS 84

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   81 VAGANTCHGSSPLVFVPRWPEVEM-SDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL 156
Cdd:PRK10506  85 VAGANTCTGSSPFVFTPPWPDVALaSDTTPSLAFYGLRNTAWAGHIRLKNSAGEWRLVISSRGRIRLCSEGESEGPL 161
 
Name Accession Description Interval E-value
PRK10506 PRK10506
prepilin peptidase-dependent protein;
1-156 8.68e-85

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 245.67  E-value: 8.68e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832937    1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISFIREGTLWCLVSS 80
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSAWGLYGWQRWQQRQRLWQTAQQLLDFLLRLQEDANWHNRDHILWLIREGQGWCLVSS 84

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   81 VAGANTCHGSSPLVFVPRWPEVEM-SDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL 156
Cdd:PRK10506  85 VAGANTCTGSSPFVFTPPWPDVALaSDTTPSLAFYGLRNTAWAGHIRLKNSAGEWRLVISSRGRIRLCSEGESEGPL 161
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-67 1.60e-12

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 59.09  E-value: 1.60e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSIS 67
Cdd:COG4970   5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTLS 71
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 6.07e-06

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 40.82  E-value: 6.07e-06
                          10        20
                  ....*....|....*....|....*..
gi 15832937     1 MKTQRGYTLIETLVAMLILVMLSASGL 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
2-25 4.68e-04

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 37.18  E-value: 4.68e-04
                         10        20
                 ....*....|....*....|....
gi 15832937    2 KTQRGYTLIETLVAMLILVMLSAS 25
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSL 24
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 4-27 7.72e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 34.97  E-value: 7.72e-04
                          10        20
                  ....*....|....*....|....
gi 15832937     4 QRGYTLIETLVAMLILVMLSASGL 27
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
ComGD NF040982
competence type IV pilus minor pilin ComGD;
4-68 2.74e-03

competence type IV pilus minor pilin ComGD;


Pssm-ID: 468912 [Multi-domain]  Cd Length: 140  Bit Score: 36.07  E-value: 2.74e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15832937    4 QRGYTLIETLVAMLILVMLSASGLYGW-QYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISF 68
Cdd:NF040982   1 IKGFTLLESLLVLLIVSSLLLLTLSLSiPQTYEQVEEKLFFEQFENDLLYAQQYAIASQQSTTITF 66
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 4-50 6.59e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 34.90  E-value: 6.59e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 15832937    4 QRGYTLIETLVAMLILVMLSA--SGLYGW-----QYWQQSQRL-WQ-TASQARDYL 50
Cdd:NF041002   1 EKGFTLLECLVALLVLSLILLliPLLIKLllkveQYLNNSDQLeWLlFLQQLESEL 56
 
Name Accession Description Interval E-value
PRK10506 PRK10506
prepilin peptidase-dependent protein;
1-156 8.68e-85

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 245.67  E-value: 8.68e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832937    1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISFIREGTLWCLVSS 80
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSAWGLYGWQRWQQRQRLWQTAQQLLDFLLRLQEDANWHNRDHILWLIREGQGWCLVSS 84

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   81 VAGANTCHGSSPLVFVPRWPEVEM-SDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL 156
Cdd:PRK10506  85 VAGANTCTGSSPFVFTPPWPDVALaSDTTPSLAFYGLRNTAWAGHIRLKNSAGEWRLVISSRGRIRLCSEGESEGPL 161
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-67 1.60e-12

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 59.09  E-value: 1.60e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSIS 67
Cdd:COG4970   5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTLS 71
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-47 1.87e-10

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 54.53  E-value: 1.87e-10
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 15832937   1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQAR 47
Cdd:COG2165   7 RRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLR 53
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
1-50 3.06e-06

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 44.40  E-value: 3.06e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   1 MKTQRGYTLIETLVAMLI--LVMLSASGLY-----GWQYWQQSQRLWQTASQARDYL 50
Cdd:COG4966   1 RRRQRGFTLVELMVALAIglIVLAAVLQLFlssrrSYRTQEALARLQENGRFALDLL 57
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 6.07e-06

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 40.82  E-value: 6.07e-06
                          10        20
                  ....*....|....*....|....*..
gi 15832937     1 MKTQRGYTLIETLVAMLILVMLSASGL 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-50 1.15e-05

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 42.31  E-value: 1.15e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15832937   1 MKTQRGYTLIETLVAMLI--LVMLSASGLY-----GWQYWQQSQRLWQTASQARDYL 50
Cdd:COG4795   5 RRRQRGFTLLELLVALAIfaLLLLAAYRGLdsvlrSRERLEQQAERLQELQRALALL 61
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-50 1.28e-05

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 41.51  E-value: 1.28e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 15832937   1 MKTQRGYTLIETLVAMLIL--VMLSASGLYGWQYwQQSQRLWQ--TASQ-ARDYL 50
Cdd:COG4967   7 RRRQRGFTLIEVLVALVILsiGLLGLAGLQAASL-RSSQDARQrtQAALlAQDLL 60
PRK10574 PRK10574
putative major pilin subunit; Provisional
 1-34 5.49e-05

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 40.79  E-value: 5.49e-05
                         10        20        30
                 ....*....|....*....|....*....|....*
gi 15832937    1 MKTQRGYTLIETLVAMLILVMLSASGLYGWQ-YWQ 34
Cdd:PRK10574   1 MDKQRGFTLIELMVVIAIIAILSAIGIPAYQnYLQ 35
PRK10557 PRK10557
prepilin peptidase-dependent protein;
1-43 4.10e-04

prepilin peptidase-dependent protein;


Pssm-ID: 236714  Cd Length: 192  Bit Score: 38.80  E-value: 4.10e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 15832937    1 MKTQRGYTLIETLVAMLI--LVMLSASGLY---GWQYWQQSQR------LWQTA 43
Cdd:PRK10557   3 PVKQRGFSLLEVLLAMAIgsVLLLGAARFLpalQRQILTLTRQlrledeIWQRV 56
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
2-25 4.68e-04

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 37.18  E-value: 4.68e-04
                         10        20
                 ....*....|....*....|....
gi 15832937    2 KTQRGYTLIETLVAMLILVMLSAS 25
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSL 24
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 4-27 7.72e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 34.97  E-value: 7.72e-04
                          10        20
                  ....*....|....*....|....
gi 15832937     4 QRGYTLIETLVAMLILVMLSASGL 27
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
1-22 1.91e-03

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 36.06  E-value: 1.91e-03
                        10        20
                ....*....|....*....|..
gi 15832937   1 MKTQRGYTLIETLVAMLILVML 22
Cdd:COG4537   8 LKKEKGFTLIEMLIVLLIISIL 29
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
1-101 2.15e-03

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 36.22  E-value: 2.15e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15832937   1 MKT-QRGYTLIETLVAMLILVMLSASGLYGWQ-YWQQSQ--RLWQTASQARDYL-LYLREDANWHNRDHSISFIREGTLW 75
Cdd:COG4969   1 MKKkQKGFTLIELMIVVAIIGILAAIAIPAYQdYVARARvsEALALASPLKTAVeECALENGSLPNCNAGDNGLPATIAT 80

                        90       100       110
                ....*....|....*....|....*....|....
gi 15832937  76 CLVSSV---AGANTCHGSSPL-----VFVPRWPE 101
Cdd:COG4969  81 KYVSSVsvdNGVITVTGTAALagltlTLTPTLNN 114
ComGD NF040982
competence type IV pilus minor pilin ComGD;
4-68 2.74e-03

competence type IV pilus minor pilin ComGD;


Pssm-ID: 468912 [Multi-domain]  Cd Length: 140  Bit Score: 36.07  E-value: 2.74e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15832937    4 QRGYTLIETLVAMLILVMLSASGLYGW-QYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISF 68
Cdd:NF040982   1 IKGFTLLESLLVLLIVSSLLLLTLSLSiPQTYEQVEEKLFFEQFENDLLYAQQYAIASQQSTTITF 66
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 4-50 6.59e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 34.90  E-value: 6.59e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 15832937    4 QRGYTLIETLVAMLILVMLSA--SGLYGW-----QYWQQSQRL-WQ-TASQARDYL 50
Cdd:NF041002   1 EKGFTLLECLVALLVLSLILLliPLLIKLllkveQYLNNSDQLeWLlFLQQLESEL 56
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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