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Conserved domains on  [gi|15833806|ref|NP_312579|]
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T3SS structure protein EscF [Escherichia coli O157:H7 str. Sakai]

Protein Classification

EscF/YscF/HrpA family type III secretion system needle major subunit( domain architecture ID 10020244)

EscF/YscF/HrpA family type III secretion system needle major subunit is a major component of the type III secretion needle structure

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
III_needle TIGR02105
type III secretion apparatus needle protein; Type III secretion systems translocate proteins, ...
1-72 1.30e-27

type III secretion apparatus needle protein; Type III secretion systems translocate proteins, usually virulence factors, out across both inner and outer membranes of certain Gram-negative bacteria and further across the plasma membrane and into the cytoplasm of the host cell. This protein, termed YscF in Yersinia, and EscF, PscF, EprI, etc. in other systems, forms the needle of the injection apparatus. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


:

Pssm-ID: 131160  Cd Length: 72  Bit Score: 94.42  E-value: 1.30e-27
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15833806    1 MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTISNRS 72
Cdd:TIGR02105  1 MDISAIAQQLKKPADDANQAVNDSLAALDLPNDPELMAELQFALNQYSAYYNIESTIVKMIKDLDSGILQKF 72
 
Name Accession Description Interval E-value
III_needle TIGR02105
type III secretion apparatus needle protein; Type III secretion systems translocate proteins, ...
1-72 1.30e-27

type III secretion apparatus needle protein; Type III secretion systems translocate proteins, usually virulence factors, out across both inner and outer membranes of certain Gram-negative bacteria and further across the plasma membrane and into the cytoplasm of the host cell. This protein, termed YscF in Yersinia, and EscF, PscF, EprI, etc. in other systems, forms the needle of the injection apparatus. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 131160  Cd Length: 72  Bit Score: 94.42  E-value: 1.30e-27
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15833806    1 MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTISNRS 72
Cdd:TIGR02105  1 MDISAIAQQLKKPADDANQAVNDSLAALDLPNDPELMAELQFALNQYSAYYNIESTIVKMIKDLDSGILQKF 72
PRK15344 PRK15344
type III secretion system needle protein SsaG; Provisional
1-68 9.55e-12

type III secretion system needle protein SsaG; Provisional


Pssm-ID: 185242  Cd Length: 71  Bit Score: 54.19  E-value: 9.55e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15833806   1 MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNdPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTI 68
Cdd:PRK15344  1 MDIAQLVDMLSHMAHQAGQAINDKMNGNDLLN-PESMIKAQFALQQYSTFINYESSLIKMIKDMLSGI 67
T3SS_needle_F pfam09392
Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF; Type III secretion systems are ...
6-68 4.21e-09

Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF; Type III secretion systems are essential virulence determinants for many gram-negative bacterial pathogens. MxiH is an extracellular alpha helical needle that is required for translocation of effector proteins into host cells. Once inside, the effector proteins subvert normal cell function to aid infection. The needle protein F, polymerizes to form a shaft.


Pssm-ID: 430579  Cd Length: 67  Bit Score: 47.28  E-value: 4.21e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15833806    6 ITQQMGEVGKTLSDSVPELLNSTDL-VNDPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTI 68
Cdd:pfam09392  1 VTNALSQGAQKAEQAVQDALNALEAdPSDPALLAKYQFKLQQYSVFINAQSTAVKAIKDILQGI 64
T3SS_SctI NF038054
type III secretion system inner rod subunit SctI; This model describes protein SctI (Secretion ...
23-52 8.47e-03

type III secretion system inner rod subunit SctI; This model describes protein SctI (Secretion and Cellular Translocation I), an inner rod protein in the basal body of the type III secretion system (T3SS) as found in many pathogenic bacteria. SctI has some sequence similarity to the needle filament protein SctF. Lineage-specific names for SctI in various bacteria include BsaK, PrgJ, and MxiI.


Pssm-ID: 411646  Cd Length: 76  Bit Score: 31.43  E-value: 8.47e-03
                        10        20        30
                ....*....|....*....|....*....|
gi 15833806  23 ELLNSTDLVNDPEKMLELQFAVQQYSAYVN 52
Cdd:NF038054 28 EKLNSPDLVSNPEKLLQLQNRLGNYSLYVS 57
 
Name Accession Description Interval E-value
III_needle TIGR02105
type III secretion apparatus needle protein; Type III secretion systems translocate proteins, ...
1-72 1.30e-27

type III secretion apparatus needle protein; Type III secretion systems translocate proteins, usually virulence factors, out across both inner and outer membranes of certain Gram-negative bacteria and further across the plasma membrane and into the cytoplasm of the host cell. This protein, termed YscF in Yersinia, and EscF, PscF, EprI, etc. in other systems, forms the needle of the injection apparatus. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 131160  Cd Length: 72  Bit Score: 94.42  E-value: 1.30e-27
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15833806    1 MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTISNRS 72
Cdd:TIGR02105  1 MDISAIAQQLKKPADDANQAVNDSLAALDLPNDPELMAELQFALNQYSAYYNIESTIVKMIKDLDSGILQKF 72
PRK15344 PRK15344
type III secretion system needle protein SsaG; Provisional
1-68 9.55e-12

type III secretion system needle protein SsaG; Provisional


Pssm-ID: 185242  Cd Length: 71  Bit Score: 54.19  E-value: 9.55e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15833806   1 MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNdPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTI 68
Cdd:PRK15344  1 MDIAQLVDMLSHMAHQAGQAINDKMNGNDLLN-PESMIKAQFALQQYSTFINYESSLIKMIKDMLSGI 67
T3SS_needle_F pfam09392
Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF; Type III secretion systems are ...
6-68 4.21e-09

Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF; Type III secretion systems are essential virulence determinants for many gram-negative bacterial pathogens. MxiH is an extracellular alpha helical needle that is required for translocation of effector proteins into host cells. Once inside, the effector proteins subvert normal cell function to aid infection. The needle protein F, polymerizes to form a shaft.


Pssm-ID: 430579  Cd Length: 67  Bit Score: 47.28  E-value: 4.21e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15833806    6 ITQQMGEVGKTLSDSVPELLNSTDL-VNDPEKMLELQFAVQQYSAYVNVESGMLKTIKDLVSTI 68
Cdd:pfam09392  1 VTNALSQGAQKAEQAVQDALNALEAdPSDPALLAKYQFKLQQYSVFINAQSTAVKAIKDILQGI 64
T3SS_SctI NF038054
type III secretion system inner rod subunit SctI; This model describes protein SctI (Secretion ...
23-52 8.47e-03

type III secretion system inner rod subunit SctI; This model describes protein SctI (Secretion and Cellular Translocation I), an inner rod protein in the basal body of the type III secretion system (T3SS) as found in many pathogenic bacteria. SctI has some sequence similarity to the needle filament protein SctF. Lineage-specific names for SctI in various bacteria include BsaK, PrgJ, and MxiI.


Pssm-ID: 411646  Cd Length: 76  Bit Score: 31.43  E-value: 8.47e-03
                        10        20        30
                ....*....|....*....|....*....|
gi 15833806  23 ELLNSTDLVNDPEKMLELQFAVQQYSAYVN 52
Cdd:NF038054 28 EKLNSPDLVSNPEKLLQLQNRLGNYSLYVS 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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