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Conserved domains on  [gi|16128260|ref|NP_414809|]
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IS1 family protein InsA [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

IS1-like element transposase( domain architecture ID 1750064)

IS1-like element transposase acting on a specific type of insertion sequences (IS), the simplest class of procaryotic transposable elements which are capable of integrating into numerous sites within genomes via a transposition pathway independent of homologous re combination.

Gene Ontology:  GO:0006313

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
transpos_IS1 super family cl41321
IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family ...
 8-91 1.99e-36

IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family elements usually through a translational frameshift mechanism.


The actual alignment was detected with superfamily member NF033558:

Pssm-ID: 468085 [Multi-domain]  Cd Length: 199  Bit Score: 121.61  E-value: 1.99e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260    8 CPSCsATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNS 87
Cdd:NF033558   1 CPRC-QSDNVVKNGKSVRGKQRYRCKDCGRQFQLDYEYRGYSEGTKEKILQLYLNGMGFRAIARVLGVSHNTVLRWLKKL 79


                 ....
gi 16128260   88 GRSR 91
Cdd:NF033558  80 GPRQ 83
 
Name Accession Description Interval E-value
transpos_IS1 NF033558
IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family ...
 8-91 1.99e-36

IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family elements usually through a translational frameshift mechanism.


Pssm-ID: 468085 [Multi-domain]  Cd Length: 199  Bit Score: 121.61  E-value: 1.99e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260    8 CPSCsATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNS 87
Cdd:NF033558   1 CPRC-QSDNVVKNGKSVRGKQRYRCKDCGRQFQLDYEYRGYSEGTKEKILQLYLNGMGFRAIARVLGVSHNTVLRWLKKL 79


                 ....
gi 16128260   88 GRSR 91
Cdd:NF033558  80 GPRQ 83
InsA COG3677
Transposase InsA [Mobilome: prophages, transposons];
1-90 7.55e-25

Transposase InsA [Mobilome: prophages, transposons];


Pssm-ID: 442893 [Multi-domain]  Cd Length: 241  Bit Score: 93.01  E-value: 7.55e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260   1 MASVSISCPSCSATDgVVRNGKSTAGHQRYLCSHCRKTWQLQFTY--TASQPGTHQKIIDMAMNGVGCRATARIMGVGLN 78
Cdd:COG3677  12 RWPNGPVCPHCGSTR-IVKNGKTRNGRQRYRCKDCGRTFTVTTGTifEGSKLPLWLQAIRLLLNGISLRQIARVLGVSYK 90

                        90
                ....*....|..
gi 16128260  79 TIFRHLKNSGRS 90
Cdd:COG3677  91 TVWRWLHRIREA 102
HTH_Tnp_IS1 pfam12759
InsA C-terminal domain; This short domain is found at the C-terminus of the InsA protein. This ...
 43-88 6.81e-24

InsA C-terminal domain; This short domain is found at the C-terminus of the InsA protein. This domain contains a helix-turn-helix domain.


Pssm-ID: 289525 [Multi-domain]  Cd Length: 46  Bit Score: 85.37  E-value: 6.81e-24
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 16128260   43 FTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNSG 88
Cdd:pfam12759  1 YTYEARKPGTKEKIIEMAMNGAGCRATARTLKVGINTVIRTLKNSR 46
transpos_ISL3 NF033550
ISL3 family transposase;
7-91 3.69e-05

ISL3 family transposase;


Pssm-ID: 468079 [Multi-domain]  Cd Length: 369  Bit Score: 40.26  E-value: 3.69e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260    7 SCPSC-SATDGVVRNGKSTAGH--------------QRYLCSHCRKTWQLQFT-------YTAsqpGTHQKIIDMAMNGV 64
Cdd:NF033550  12 TCPECgKPSRRVHDTGKRRIRHlpifgrpvylelrvRRFKCPECGKTFTEELPwarkrsrITL---RLEAAVLALLLELM 88

                         90       100
                 ....*....|....*....|....*..
gi 16128260   65 GCRATARIMGVGLNTIFRHLKNSGRSR 91
Cdd:NF033550  89 SVAAVARQLGVSWSTVWRILLRAVRRL 115
 
Name Accession Description Interval E-value
transpos_IS1 NF033558
IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family ...
 8-91 1.99e-36

IS1 family transposase; Proteins of this family are DDE transposases encoded by the IS1 family elements usually through a translational frameshift mechanism.


Pssm-ID: 468085 [Multi-domain]  Cd Length: 199  Bit Score: 121.61  E-value: 1.99e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260    8 CPSCsATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNS 87
Cdd:NF033558   1 CPRC-QSDNVVKNGKSVRGKQRYRCKDCGRQFQLDYEYRGYSEGTKEKILQLYLNGMGFRAIARVLGVSHNTVLRWLKKL 79


                 ....
gi 16128260   88 GRSR 91
Cdd:NF033558  80 GPRQ 83
InsA COG3677
Transposase InsA [Mobilome: prophages, transposons];
1-90 7.55e-25

Transposase InsA [Mobilome: prophages, transposons];


Pssm-ID: 442893 [Multi-domain]  Cd Length: 241  Bit Score: 93.01  E-value: 7.55e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260   1 MASVSISCPSCSATDgVVRNGKSTAGHQRYLCSHCRKTWQLQFTY--TASQPGTHQKIIDMAMNGVGCRATARIMGVGLN 78
Cdd:COG3677  12 RWPNGPVCPHCGSTR-IVKNGKTRNGRQRYRCKDCGRTFTVTTGTifEGSKLPLWLQAIRLLLNGISLRQIARVLGVSYK 90

                        90
                ....*....|..
gi 16128260  79 TIFRHLKNSGRS 90
Cdd:COG3677  91 TVWRWLHRIREA 102
HTH_Tnp_IS1 pfam12759
InsA C-terminal domain; This short domain is found at the C-terminus of the InsA protein. This ...
 43-88 6.81e-24

InsA C-terminal domain; This short domain is found at the C-terminus of the InsA protein. This domain contains a helix-turn-helix domain.


Pssm-ID: 289525 [Multi-domain]  Cd Length: 46  Bit Score: 85.37  E-value: 6.81e-24
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 16128260   43 FTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNSG 88
Cdd:pfam12759  1 YTYEARKPGTKEKIIEMAMNGAGCRATARTLKVGINTVIRTLKNSR 46
Zn_Tnp_IS1 pfam03811
InsA N-terminal domain; This appears to be a short zinc binding domain found in IS1 InsA ...
 1-36 6.45e-11

InsA N-terminal domain; This appears to be a short zinc binding domain found in IS1 InsA family protein. It is found at the N-terminus of the protein and may be a DNA-binding domain.


Pssm-ID: 281762 [Multi-domain]  Cd Length: 35  Bit Score: 52.21  E-value: 6.45e-11
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 16128260    1 MASVSISCPSCSATDgVVRNGKSTAGHQRYLCSHCR 36
Cdd:pfam03811  1 MASVSIHCPRCSSTD-VYRHGKSTAGHQRFRCRHCR 35
transpos_ISL3 NF033550
ISL3 family transposase;
7-91 3.69e-05

ISL3 family transposase;


Pssm-ID: 468079 [Multi-domain]  Cd Length: 369  Bit Score: 40.26  E-value: 3.69e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128260    7 SCPSC-SATDGVVRNGKSTAGH--------------QRYLCSHCRKTWQLQFT-------YTAsqpGTHQKIIDMAMNGV 64
Cdd:NF033550  12 TCPECgKPSRRVHDTGKRRIRHlpifgrpvylelrvRRFKCPECGKTFTEELPwarkrsrITL---RLEAAVLALLLELM 88

                         90       100
                 ....*....|....*....|....*..
gi 16128260   65 GCRATARIMGVGLNTIFRHLKNSGRSR 91
Cdd:NF033550  89 SVAAVARQLGVSWSTVWRILLRAVRRL 115
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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