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Conserved domains on  [gi|25152290|ref|NP_509853|]
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ADP-ribosylation factor-binding protein GGA1 [Caenorhabditis elegans]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
VHS_ENTH_ANTH super family cl02544
VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a ...
 8-147 2.43e-58

VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a VHS, CID, ENTH, or ANTH domain. The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It is located at the N-termini of proteins involved in intracellular membrane trafficking. The CTD-Interacting Domain (CID) is present in several RNA-processing factors and binds tightly to the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP II or Pol II). The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. A set of proteins previously designated as harboring an ENTH domain in fact contains a highly similar, yet unique module referred to as an AP180 N-Terminal Homology (ANTH) domain. VHS, ENTH, and ANTH domains are structurally similar and are composed of a superhelix of eight alpha helices. ENTH and ANTH (E/ANTH) domains bind both inositol phospholipids and proteins and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. E/ANTH domain-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


The actual alignment was detected with superfamily member cd03567:

Pssm-ID: 470608  Cd Length: 139  Bit Score: 191.26  E-value: 2.43e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290   8 PIDYWVCRATDRFIgDEERIKALDFIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGR 87
Cdd:cd03567   1 SLEYLLNKATNPFN-TEEDWEAIDAFCDRVNKEPEGPQIAVRLLAHKIQSPNEKEALRALTVLEACVKNCGPRFHSEVGK 79

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  88 YKFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIITD 147
Cdd:cd03567  80 FRFLNELIKLVSPKYLGNRTPEEVKQKIIELLYSWTRSLPHEPKIKEAYDMLKKQGIIKE 139
GGA_N-GAT pfam18308
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 169-207 5.21e-13

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


:

Pssm-ID: 465702  Cd Length: 39  Bit Score: 63.09  E-value: 5.21e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 25152290   169 FDNEEQAVLLKALLSSNRPEDLETANNLIKTLVETEEHK 207
Cdd:pfam18308   1 FDDEEKSKLLARLLKSKNPEDLQAANRLIKSMVKEDDER 39
 
Name Accession Description Interval E-value
VHS_GGA_metazoan cd03567
VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, ...
 8-147 2.43e-58

VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Jawed vertebrates contain as many as three GGA proteins: GGA1, GGA2, and GGA3. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340769  Cd Length: 139  Bit Score: 191.26  E-value: 2.43e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290   8 PIDYWVCRATDRFIgDEERIKALDFIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGR 87
Cdd:cd03567   1 SLEYLLNKATNPFN-TEEDWEAIDAFCDRVNKEPEGPQIAVRLLAHKIQSPNEKEALRALTVLEACVKNCGPRFHSEVGK 79

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  88 YKFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIITD 147
Cdd:cd03567  80 FRFLNELIKLVSPKYLGNRTPEEVKQKIIELLYSWTRSLPHEPKIKEAYDMLKKQGIIKE 139
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 8-146 7.80e-41

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 144.36  E-value: 7.80e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290     8 PIDYWVCRATDRFIGDEE---RIKALDFIIESiykqPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHER 84
Cdd:pfam00790   1 PLERWIDKATDPSLREEDwslILDICDLINET----ETGPKEAVRLIKKRINSPNPHVALLALTLLDACVKNCGSKFHLE 76

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 25152290    85 CGRYKFLNELVRLIAPKYNGKLTSdaLKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIIT 146
Cdd:pfam00790  77 IASKEFLNELVKLIPNKPYGKPAK--VKKKILELIQEWADAFKNDPDLKYIRDMYKLLKRKG 136
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 9-145 6.78e-36

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 130.88  E-value: 6.78e-36
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290      9 IDYWVCRATDRFIGDEERIKALDFIIESIYKqPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRY 88
Cdd:smart00288   1 VERLIDKATSPSLLEEDWELILEICDLINST-PDGPKDAVRLLKKRLNNKNPHVALLALTLLDACVKNCGSKFHLEVASK 79

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 25152290     89 KFLNELVRLIAPKYNGKLtsdaLKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKII 145
Cdd:smart00288  80 EFLNELVKLIKPKYPLPL----VKKRILELIQEWADAFKNDPDLSQIVDVYDLLKKK 132
GGA_N-GAT pfam18308
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 169-207 5.21e-13

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


Pssm-ID: 465702  Cd Length: 39  Bit Score: 63.09  E-value: 5.21e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 25152290   169 FDNEEQAVLLKALLSSNRPEDLETANNLIKTLVETEEHK 207
Cdd:pfam18308   1 FDDEEKSKLLARLLKSKNPEDLQAANRLIKSMVKEDDER 39
 
Name Accession Description Interval E-value
VHS_GGA_metazoan cd03567
VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, ...
 8-147 2.43e-58

VHS (Vps27/Hrs/STAM) domain of metazoan GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. Jawed vertebrates contain as many as three GGA proteins: GGA1, GGA2, and GGA3. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340769  Cd Length: 139  Bit Score: 191.26  E-value: 2.43e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290   8 PIDYWVCRATDRFIgDEERIKALDFIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGR 87
Cdd:cd03567   1 SLEYLLNKATNPFN-TEEDWEAIDAFCDRVNKEPEGPQIAVRLLAHKIQSPNEKEALRALTVLEACVKNCGPRFHSEVGK 79

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  88 YKFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIITD 147
Cdd:cd03567  80 FRFLNELIKLVSPKYLGNRTPEEVKQKIIELLYSWTRSLPHEPKIKEAYDMLKKQGIIKE 139
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 8-146 7.80e-41

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 144.36  E-value: 7.80e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290     8 PIDYWVCRATDRFIGDEE---RIKALDFIIESiykqPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHER 84
Cdd:pfam00790   1 PLERWIDKATDPSLREEDwslILDICDLINET----ETGPKEAVRLIKKRINSPNPHVALLALTLLDACVKNCGSKFHLE 76

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 25152290    85 CGRYKFLNELVRLIAPKYNGKLTSdaLKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIIT 146
Cdd:pfam00790  77 IASKEFLNELVKLIPNKPYGKPAK--VKKKILELIQEWADAFKNDPDLKYIRDMYKLLKRKG 136
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 9-145 6.78e-36

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 130.88  E-value: 6.78e-36
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290      9 IDYWVCRATDRFIGDEERIKALDFIIESIYKqPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRY 88
Cdd:smart00288   1 VERLIDKATSPSLLEEDWELILEICDLINST-PDGPKDAVRLLKKRLNNKNPHVALLALTLLDACVKNCGSKFHLEVASK 79

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 25152290     89 KFLNELVRLIAPKYNGKLtsdaLKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKII 145
Cdd:smart00288  80 EFLNELVKLIKPKYPLPL----VKKRILELIQEWADAFKNDPDLSQIVDVYDLLKKK 132
VHS_GGA3 cd17008
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA3; ADP-ribosylation ...
 9-146 4.65e-23

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA3; ADP-ribosylation factor-binding protein GGA3 (Golgi-localized, Gamma-ear-containing, Arf-binding 3) regulates the trafficking and is required for the lysosomal degradation of BACE (beta-site APP-cleaving enzyme), the protease that initiates the production of beta-amyloid, which causes Alzheimer's disease. It also plays a key role in GABA (+) transmission, which is important in the regulation of anxiety-like behaviors. GGA3 is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340805 [Multi-domain]  Cd Length: 141  Bit Score: 95.10  E-value: 4.65e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290   9 IDYWVCRATDrfigDEERIKALDFII---ESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERC 85
Cdd:cd17008   4 LESWLNKATN----PSNRQEDWEYIIgfcDQINKELEGPQIAVRLLAHKIQSPQEWEALQALTVLEACMKNCGRRFHNEV 79

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 25152290  86 GRYKFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKIIT 146
Cdd:cd17008  80 GKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTVALPEEAKIKDAYHMLKRQGIVQ 140
VHS_GGA2 cd17010
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation ...
 9-145 2.18e-22

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA2 (Golgi-localized, Gamma-ear-containing, Arf-binding 2) is also called Gamma-adaptin-related protein 2 and VHS domain and ear domain of gamma-adaptin (Vear). It is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340807  Cd Length: 139  Bit Score: 93.41  E-value: 2.18e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290   9 IDYWVCRATDRfIGDEERIKALDFIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRY 88
Cdd:cd17010   2 LESWLNKATDP-SNSEEDWECIQGFCEQVNKEPEGPQIATRLLAHKIQSPQEREALHALTVLEMCMNNCGERFHSEVAKF 80

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 25152290  89 KFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLKLSKII 145
Cdd:cd17010  81 RFLNELIKVLSPKYLGTWSSEKVKSRVIEVLFSWTVWLPEEVKIRDAYQMLKKQGII 137
VHS_GGA1 cd17009
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation ...
 35-145 4.09e-20

VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA1 (Golgi-localized, Gamma-ear-containing, Arf-binding 1) is also called Gamma-adaptin-related protein 1. It is expressed in human brain and affects the generation of amyloid beta-peptide, and may be involved in the pathogenesis of Alzheimer disease. It is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340806 [Multi-domain]  Cd Length: 139  Bit Score: 86.96  E-value: 4.09e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  35 ESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRYKFLNELVRLIAPKYNGKLTSDALKTE 114
Cdd:cd17009  27 EQLNEEFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTPEKVKNK 106

                        90       100       110
                ....*....|....*....|....*....|.
gi 25152290 115 IIKLLFIWQLSIKHIPKYKQVYESLKLSKII 145
Cdd:cd17009 107 ILELLYSWTVGLPEEVKIAEAYQMLKKQGIV 137
VHS_GGA cd16977
VHS (Vps27/Hrs/STAM) domain of GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) ...
 12-140 2.21e-19

VHS (Vps27/Hrs/STAM) domain of GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) subfamily; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprises a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.


Pssm-ID: 340774  Cd Length: 133  Bit Score: 84.54  E-value: 2.21e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  12 WVCRATDRFIGDEERIKALDFIiESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRYKFL 91
Cdd:cd16977   4 WINRATNPSNAEPDWALILGFC-DQINEDGEGPRDAARLLAHKIQSPQEWEAIQALTVLEVCMKNCGYRFHLEVGKFRFL 82

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*....
gi 25152290  92 NELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIKHIPKYKQVYESLK 140
Cdd:cd16977  83 NELIKVVSPKYLGSRRSEKVKRKILELLYSWTVTLPEEGKIRDAYQMLK 131
GGA_N-GAT pfam18308
N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA ...
 169-207 5.21e-13

N-terminal extension of GAT domain; This region is found in the N-terminal region of the GGA and Tom1 (GAT) domain in Golgi-localizing gamma-adaptin ARF-binding protein 1 (GGA1) present in Homo sapiens. The GAT domain is the key region in GGA that interacts with ARF. ARF plays a crucial role in docking adaptor proteins to membranes. This region is referred to as N-GAT and it interacts extensively with ARF.


Pssm-ID: 465702  Cd Length: 39  Bit Score: 63.09  E-value: 5.21e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 25152290   169 FDNEEQAVLLKALLSSNRPEDLETANNLIKTLVETEEHK 207
Cdd:pfam18308   1 FDDEEKSKLLARLLKSKNPEDLQAANRLIKSMVKEDDER 39
VHS_ENTH_ANTH cd00197
VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a ...
 12-122 1.39e-10

VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a VHS, CID, ENTH, or ANTH domain. The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It is located at the N-termini of proteins involved in intracellular membrane trafficking. The CTD-Interacting Domain (CID) is present in several RNA-processing factors and binds tightly to the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP II or Pol II). The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. A set of proteins previously designated as harboring an ENTH domain in fact contains a highly similar, yet unique module referred to as an AP180 N-Terminal Homology (ANTH) domain. VHS, ENTH, and ANTH domains are structurally similar and are composed of a superhelix of eight alpha helices. ENTH and ANTH (E/ANTH) domains bind both inositol phospholipids and proteins and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. E/ANTH domain-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340764  Cd Length: 115  Bit Score: 58.59  E-value: 1.39e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  12 WVCRATDRFIGDEERiKALDFIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRYKFL 91
Cdd:cd00197   4 TVEKATSNENMGPDW-PLIMEICDLINETNVGPKEAVDAIKKRINNKNPHVVLKALTLLEYCVKNCGERFHQEVASNDFA 82

                        90       100       110
                ....*....|....*....|....*....|.
gi 25152290  92 NELVRLIAPKYNGKLTSDALKTEIIKLLFIW 122
Cdd:cd00197  83 VELLKFDKSGLLGDDVSTNVREKAIELVQLW 113
VHS cd03561
VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein ...
 32-140 1.38e-08

VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It has a superhelical structure similar to that of the ARM (Armadillo) repeats and is present at the N-termini of proteins involved in intracellular membrane trafficking. There are four general groups of VHS domain containing proteins based on their association with other domains. The first group consists of proteins of the STAM/EAST/Hbp family, which has the domain composition of VHS-SH3-ITAM. The second consists of proteins with a FYVE domain C-terminal to VHS. The third consists of GGA proteins with a domain composition of VHS-GAT (GGA and TOM)-GAE (Gamma-Adaptin Ear) domain. The fourth consists of proteins with a VHS domain alone or with domains other than those mentioned above. In GGA proteins, VHS domains are involved in cargo recognition in trans-Golgi, thereby having a general membrane targeting/cargo recognition role in vesicular trafficking.


Pssm-ID: 340765  Cd Length: 131  Bit Score: 53.42  E-value: 1.38e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  32 FIIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRYKFLNELVRLIapkyNGKLTSDAL 111
Cdd:cd03561  23 EICDLVNSDPAQAKDAVRALRKRLQSKNPKVQLLALTLLETLVKNCGAPFHSEVASRDFLQELVKLV----KKKKTSPEV 98

                        90       100       110
                ....*....|....*....|....*....|..
gi 25152290 112 KTEIIKLLFIWQLSI---KHIPKYKQVYESLK 140
Cdd:cd03561  99 REKALALIQEWAEAFqgdGRLPLFYDTYQQLR 130
VHS_Tom1_like cd03565
VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily; This subfamily is composed of Tom1 (Target of ...
 62-140 1.17e-05

VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily; This subfamily is composed of Tom1 (Target of myb1 - retroviral oncogene) protein, Tom1L1 (Tom1-like1), Tom1L2 (Tom1-like2), and similar proteins. Proteins belonging to this subfamily are characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. They are novel regulators for post-Golgi trafficking and signaling. Yeast do not contain homologous proteins of the Tom1 subfamily, suggesting these proteins have evolved to accommodate more complex cellular processes. Tom1 is essential for the negative regulation of Interleukin-1 and Tumor Necrosis Factor-induced signaling pathways. The VHS domain has a superhelical structure similar to the structure of the ARM repeats and is present at the very N-termini of proteins. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340768  Cd Length: 138  Bit Score: 45.33  E-value: 1.17e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  62 EAMLTIRALDYLVRNGGEKVHERCGRYKFLNELVRLIAPKYNgklTSDALKTEIIKLLFIWQLSIKHIPKYK---QVYES 138
Cdd:cd03565  57 VVMYTLTLLETCVKNCGHRFHVLVANKDFLQELVKLIGPKND---PPTIVQEKVLSLIQSWADAFRGQPDLQevvKVYQE 133


                ..
gi 25152290 139 LK 140
Cdd:cd03565 134 LK 135
VHS_Hrs cd03569
VHS (Vps27/Hrs/STAM) domain of Hepatocyte growth factor-regulated tyrosine kinase substrate, ...
 33-142 1.50e-04

VHS (Vps27/Hrs/STAM) domain of Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs; Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) plays a role in at least three vesicle trafficking events: exocytosis, endocytosis, and endosome to lysosome trafficking. Hrs is involved in promoting rapid recycling of endocytosed signaling receptors to the plasma membrane. Together with STAM or STAM2, it comprises the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery, which functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles. Hrs contains an N-terminal VHS domain, which has a superhelical structure similar to the structure of ARM (Armadillo) repeats, a FYVE (Fab1p, YOTB, Vac1p, and EEA1) zinc finger domain, a Double Ubiquitin-Interacting Motif (DUIM), a P(S/T)XP motif that recruit ESCRT-I, a GAT (GGA and TOM) domain, and a short peptide motif near the C-terminus that recruits clathrin.


Pssm-ID: 340771  Cd Length: 138  Bit Score: 41.85  E-value: 1.50e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  33 IIESIYKQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGRYKFLNELVRLIApkyngKLTSDALK 112
Cdd:cd03569  26 ICDLIRQGDVTPKYAVSAIKKKLNDQNPHVALYALQVLESVVKNCGTPVHDEVATKEFMEELRDLVK-----TTTSENVR 100

                        90       100       110
                ....*....|....*....|....*....|...
gi 25152290 113 TEIIKLLFIWQLSIKHIPKYKQV---YESLKLS 142
Cdd:cd03569 101 NKILELIQAWAHAFRNEPKYKIVqdtYNIMKME 133
VHS_Lsb5 cd16980
VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein ...
 54-140 1.54e-04

VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein 5 (LAS17-binding protein 5, Lsb5, or Lsb5p) localizes to the plasma membrane and plays a role in endocytosis in yeast. It interacts with actin regulators Sla1p and Las17p, ubiquitin, and Arf3p, coupling actin dynamics to membrane trafficking processes. Lsb5p contains an N-terminal VHS domain and a GAT (GGA and TOM) domain. The VHS domain has a superhelical structure similar to the structure of ARM (Armadillo) repeats. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340777  Cd Length: 132  Bit Score: 41.82  E-value: 1.54e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25152290  54 KLSSPSQEEAMLTIraLDYLVRNGGEKVHERCGRYKFLNELVRLIApkyNGKLTSDALKTEIIKLLFIWQLSIKHIPKYK 133
Cdd:cd16980  47 KYGNPHQQLRALTL--LDLLVENGGKKFQAQFASDKKLEDLLRLIA---SDPSTDPKVRKKLLELLGGWAYEFKGDPGLS 121

                        90
                ....*....|
gi 25152290 134 ---QVYESLK 140
Cdd:cd16980 122 giaSLYKKVK 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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