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Conserved domains on  [gi|19111918|ref|NP_595126|]
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phospholipid-translocating ATPase complex Lem3 family subunit [Schizosaccharomyces pombe]

Protein Classification

CDC50/LEM3 family protein( domain architecture ID 10009045)

CDC50/LEM3 family protein similar to Saccharomyces cerevisiae cell division control protein 50, Alkylphosphocholine resistance protein LEM3 and phospholipid translocase non-catalytic subunit CRF1

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
2-384 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


:

Pssm-ID: 227368  Cd Length: 372  Bit Score: 599.55  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   2 PEALNENVSDTASNGPVAKTRAPPNTSFRQQRIKSWQPLLTPKIVLPLFFVLGIIFGPLGGGLLYASSIVQELVVDYTDC 81
Cdd:COG5035   1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918  82 ETLASyDEFSAVPSKKYTASFDQsgtigFDKESTYWKLEKILDKDLDMdvnyCIIRFTVPSVLKAPIFIYYRLTNFFQNH 161
Cdd:COG5035  81 MTLAS-DEFSDIPSEYIQFHFKK-----KVNVLPQWRFSTDEEDDFQK----CQIRFTVPSDMKKPVFIYYRLTNFYQNH 150
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 162 RRYAKSVDEKQLQGVALTADEVKGgNCFPLEVNEDdKPYYPCGLIANSLFNDTFSS-LRLLDDNSVYTFSTKNIAWASDK 240
Cdd:COG5035 151 RRYVKSFDEDQLRGEALKSDDLKS-NCKPLSYNED-KIIYPCGLIANSMFNDTFSSlLTGIEDTSNYNLTTKGIAWESDR 228
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 241 RRFLKTNYSPDDVAPPPNWVLRYPDGYTESNMPDLSTMENLQVWMRTAGLPTFSKLAMRNDNDDIFPGTYEIKIGLFFPV 320
Cdd:COG5035 229 HRYKKTKYNASDIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPV 308
                       330       340       350       360       370       380
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 19111918 321 KSFDGTKSLVLTTRSVLGGKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRPRKLADHRYLNWDSE 384
Cdd:COG5035 309 LEFNGTKSIVLTTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNWNME 372
 
Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
2-384 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 599.55  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   2 PEALNENVSDTASNGPVAKTRAPPNTSFRQQRIKSWQPLLTPKIVLPLFFVLGIIFGPLGGGLLYASSIVQELVVDYTDC 81
Cdd:COG5035   1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918  82 ETLASyDEFSAVPSKKYTASFDQsgtigFDKESTYWKLEKILDKDLDMdvnyCIIRFTVPSVLKAPIFIYYRLTNFFQNH 161
Cdd:COG5035  81 MTLAS-DEFSDIPSEYIQFHFKK-----KVNVLPQWRFSTDEEDDFQK----CQIRFTVPSDMKKPVFIYYRLTNFYQNH 150
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 162 RRYAKSVDEKQLQGVALTADEVKGgNCFPLEVNEDdKPYYPCGLIANSLFNDTFSS-LRLLDDNSVYTFSTKNIAWASDK 240
Cdd:COG5035 151 RRYVKSFDEDQLRGEALKSDDLKS-NCKPLSYNED-KIIYPCGLIANSMFNDTFSSlLTGIEDTSNYNLTTKGIAWESDR 228
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 241 RRFLKTNYSPDDVAPPPNWVLRYPDGYTESNMPDLSTMENLQVWMRTAGLPTFSKLAMRNDNDDIFPGTYEIKIGLFFPV 320
Cdd:COG5035 229 HRYKKTKYNASDIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPV 308
                       330       340       350       360       370       380
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 19111918 321 KSFDGTKSLVLTTRSVLGGKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRPRKLADHRYLNWDSE 384
Cdd:COG5035 309 LEFNGTKSIVLTTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNWNME 372
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
64-372 8.20e-144

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 410.07  E-value: 8.20e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918    64 LLYASSIVQELVVDYT-DCETLASYDEFSAVPSKKYTASfdqsgtigfdkestywklekildkdldmdVNYCIIRFTVPS 142
Cdd:pfam03381   1 LLYASNNVQEIVIDYTdDCATLAPTDNFNPIDYIQNSNE-----------------------------TKTCTIQFTIPK 51
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   143 VLKAPIFIYYRLTNFFQNHRRYAKSVDEKQLQGVALTADEvKGGNCFPLEVNE---DDKPYYPCGLIANSLFNDTF---- 215
Cdd:pfam03381  52 DMKGPVYLYYKLTNFYQNHRRYVKSRDDDQLKGKAVSALL-SGSSCKPLRYNGardDGKPIYPCGLIANSMFNDTFtlyn 130
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   216 --SSLRLLDDNSVYTFSTKNIAWASDK-RRF-LKTNYSPDDVAPPPNWVLRYPDGYTESNMPDLSTMENLQVWMRTAGLP 291
Cdd:pfam03381 131 nsSPLLLNVGTSTYPLTKKGIAWPSDKeHKFkNPTKYNASDIVPPPNWQKRYPNGYTEDNIPNLSENEDFIVWMRTAALP 210
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   292 TFSKLAMR-NDNDDIFPGTYEIKIGLFFPVKSFDGTKSLVLTTRSVLGGKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRP 370
Cdd:pfam03381 211 TFRKLYRRiNDTDGLPAGTYTIDIGYNYPVTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKP 290

                  ..
gi 19111918   371 RK 372
Cdd:pfam03381 291 RK 292
 
Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
2-384 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 599.55  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   2 PEALNENVSDTASNGPVAKTRAPPNTSFRQQRIKSWQPLLTPKIVLPLFFVLGIIFGPLGGGLLYASSIVQELVVDYTDC 81
Cdd:COG5035   1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918  82 ETLASyDEFSAVPSKKYTASFDQsgtigFDKESTYWKLEKILDKDLDMdvnyCIIRFTVPSVLKAPIFIYYRLTNFFQNH 161
Cdd:COG5035  81 MTLAS-DEFSDIPSEYIQFHFKK-----KVNVLPQWRFSTDEEDDFQK----CQIRFTVPSDMKKPVFIYYRLTNFYQNH 150
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 162 RRYAKSVDEKQLQGVALTADEVKGgNCFPLEVNEDdKPYYPCGLIANSLFNDTFSS-LRLLDDNSVYTFSTKNIAWASDK 240
Cdd:COG5035 151 RRYVKSFDEDQLRGEALKSDDLKS-NCKPLSYNED-KIIYPCGLIANSMFNDTFSSlLTGIEDTSNYNLTTKGIAWESDR 228
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918 241 RRFLKTNYSPDDVAPPPNWVLRYPDGYTESNMPDLSTMENLQVWMRTAGLPTFSKLAMRNDNDDIFPGTYEIKIGLFFPV 320
Cdd:COG5035 229 HRYKKTKYNASDIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPV 308
                       330       340       350       360       370       380
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 19111918 321 KSFDGTKSLVLTTRSVLGGKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRPRKLADHRYLNWDSE 384
Cdd:COG5035 309 LEFNGTKSIVLTTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNWNME 372
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
64-372 8.20e-144

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 410.07  E-value: 8.20e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918    64 LLYASSIVQELVVDYT-DCETLASYDEFSAVPSKKYTASfdqsgtigfdkestywklekildkdldmdVNYCIIRFTVPS 142
Cdd:pfam03381   1 LLYASNNVQEIVIDYTdDCATLAPTDNFNPIDYIQNSNE-----------------------------TKTCTIQFTIPK 51
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   143 VLKAPIFIYYRLTNFFQNHRRYAKSVDEKQLQGVALTADEvKGGNCFPLEVNE---DDKPYYPCGLIANSLFNDTF---- 215
Cdd:pfam03381  52 DMKGPVYLYYKLTNFYQNHRRYVKSRDDDQLKGKAVSALL-SGSSCKPLRYNGardDGKPIYPCGLIANSMFNDTFtlyn 130
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   216 --SSLRLLDDNSVYTFSTKNIAWASDK-RRF-LKTNYSPDDVAPPPNWVLRYPDGYTESNMPDLSTMENLQVWMRTAGLP 291
Cdd:pfam03381 131 nsSPLLLNVGTSTYPLTKKGIAWPSDKeHKFkNPTKYNASDIVPPPNWQKRYPNGYTEDNIPNLSENEDFIVWMRTAALP 210
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19111918   292 TFSKLAMR-NDNDDIFPGTYEIKIGLFFPVKSFDGTKSLVLTTRSVLGGKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRP 370
Cdd:pfam03381 211 TFRKLYRRiNDTDGLPAGTYTIDIGYNYPVTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKP 290

                  ..
gi 19111918   371 RK 372
Cdd:pfam03381 291 RK 292
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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