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Conserved domains on  [gi|19113173|ref|NP_596381|]
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proteasome regulatory subunit Rpn2 [Schizosaccharomyces pombe]

Protein Classification

26S proteasome non-ATPase regulatory subunit 1; 3-phosphoglycerate dehydrogenase family protein( domain architecture ID 11473818)

26S proteasome non-ATPase regulatory subunit 1 (PSMD1), also known as RPN2, is a non-ATPase subunit of the 19S regulatory complex of the 26S proteasome which plays a critical role in the recognition of ubiquitinated protein substrates by the proteasome and is involved in the ATP-dependent unfolding and translocation of these substrates into the proteasome core for degradation| 3-phosphoglycerate dehydrogenase family protein may catalyze the initial step in the biosynthesis of L-serine from D-3-phosphoglycerate; also contains 26S proteasome regulatory subunit RPN2 C-terminal domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RPN2 COG5116
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 10-962 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 1528.73  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  10 SIITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAALVVSKVYYYLGEYDEALL 89
Cdd:COG5116   1 MSMTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  90 FALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKADPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLD 169
Cdd:COG5116  81 YALRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDSAYIGGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 170 IIEYILNADKETDLKPYVLELAMTVVLDIEFRNRLLRLLLSSFLTETEPDYFSVGKCVVHLNDASVAAKLLMKLSSQNDD 249
Cdd:COG5116 161 IIEKYLSDGNDCDIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKENDL 240

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 250 KslLTAyQLAFDLEDSAPQEFLnsvmDLLPSPSVANSEEDAnadskkedsspcgyIIRILSGEQTVKYDREFLYAHNNTD 329
Cdd:COG5116 241 L--LYA-QVAFDLEDSASQEIL----EILVTELVAQGYDQA--------------VMSILSGEFTKKYLGAFLLEKNNTD 299

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 330 MLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYL 409
Cdd:COG5116 300 FKFLNSSKSSLARKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNWAKFTATASLGVIHLGNSNPGYEILKPYL 379

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 410 PEEDApsSSTYSEGGAFYAMGLIHANHGRGVTEYLREQLKHTEDE---IVQYGLLLGIGLTGMASRDETLYESVKTILFN 486
Cdd:COG5116 380 PSEVA--SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDEltpELAYGVCLGIGLINMGSANREIYEKLKELLKN 457

                       490       500       510       520       530       540       550       560
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 487 DNAVAGSAAGISMGLIMLGTASSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGM 566
Cdd:COG5116 458 DRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALILYGRQEMADDYINELLYDKDSILRYNGV 537

                       570       580       590       600       610       620       630       640
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 567 FATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVELLVDSYNPHVRYGSAIALGIACANS 646
Cdd:COG5116 538 FSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGTVELLSESHNFHVRAGVAVALGIACAGT 617

                       650       660       670       680       690       700       710       720
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 647 GSNAALDLLSRLVEDATDFVRQGAMIAQAMILTQHNDQLNSKVSGIRKHFEQVINEKHEDALAKLGATLAQGIIDAGGRN 726
Cdd:COG5116 618 GDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRVIVDKHESGLAKLGAVLAQGISEAGGRN 697

                       730       740       750       760       770       780       790       800
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 727 VTIALQTATGSLKLSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAK 806
Cdd:COG5116 698 VTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQAIPKFCFNEDLEEEEFEYPRMYEEASGK 777

                       810       820       830       840       850       860       870       880
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 807 TVQKVETAVLSTTVKAQARAKRAEREkasKGSNDDEMKIDKKTTE-EKEATPMEMDEEKSQDISINGNSKKEEPksETLE 885
Cdd:COG5116 778 SVRKVNTAVLSTTIKAAARAKQKPKE---KGPNDKEIKIESPSVEtEGERCTIKQREEKGIDAPAILNVKKKKP--YKVD 852

                       890       900       910       920       930       940       950
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19113173 886 NFTRVVPAQLPYISFNLNGRYYPVRKFTGGVLMLIDRESDKAPDLIELNRDAVPASAdtePGEQEASPPEDFEYPFD 962
Cdd:COG5116 853 NMTRILPQQSRYISFIKDDRFVPVRKFKGGVVVLRDREPKEPVALIETVRQMKDVNA---PLPTPFKVDDNVDFPSA 926
 
Name Accession Description Interval E-value
RPN2 COG5116
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 10-962 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 1528.73  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  10 SIITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAALVVSKVYYYLGEYDEALL 89
Cdd:COG5116   1 MSMTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  90 FALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKADPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLD 169
Cdd:COG5116  81 YALRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDSAYIGGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 170 IIEYILNADKETDLKPYVLELAMTVVLDIEFRNRLLRLLLSSFLTETEPDYFSVGKCVVHLNDASVAAKLLMKLSSQNDD 249
Cdd:COG5116 161 IIEKYLSDGNDCDIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKENDL 240

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 250 KslLTAyQLAFDLEDSAPQEFLnsvmDLLPSPSVANSEEDAnadskkedsspcgyIIRILSGEQTVKYDREFLYAHNNTD 329
Cdd:COG5116 241 L--LYA-QVAFDLEDSASQEIL----EILVTELVAQGYDQA--------------VMSILSGEFTKKYLGAFLLEKNNTD 299

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 330 MLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYL 409
Cdd:COG5116 300 FKFLNSSKSSLARKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNWAKFTATASLGVIHLGNSNPGYEILKPYL 379

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 410 PEEDApsSSTYSEGGAFYAMGLIHANHGRGVTEYLREQLKHTEDE---IVQYGLLLGIGLTGMASRDETLYESVKTILFN 486
Cdd:COG5116 380 PSEVA--SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDEltpELAYGVCLGIGLINMGSANREIYEKLKELLKN 457

                       490       500       510       520       530       540       550       560
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 487 DNAVAGSAAGISMGLIMLGTASSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGM 566
Cdd:COG5116 458 DRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALILYGRQEMADDYINELLYDKDSILRYNGV 537

                       570       580       590       600       610       620       630       640
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 567 FATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVELLVDSYNPHVRYGSAIALGIACANS 646
Cdd:COG5116 538 FSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGTVELLSESHNFHVRAGVAVALGIACAGT 617

                       650       660       670       680       690       700       710       720
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 647 GSNAALDLLSRLVEDATDFVRQGAMIAQAMILTQHNDQLNSKVSGIRKHFEQVINEKHEDALAKLGATLAQGIIDAGGRN 726
Cdd:COG5116 618 GDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRVIVDKHESGLAKLGAVLAQGISEAGGRN 697

                       730       740       750       760       770       780       790       800
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 727 VTIALQTATGSLKLSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAK 806
Cdd:COG5116 698 VTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQAIPKFCFNEDLEEEEFEYPRMYEEASGK 777

                       810       820       830       840       850       860       870       880
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 807 TVQKVETAVLSTTVKAQARAKRAEREkasKGSNDDEMKIDKKTTE-EKEATPMEMDEEKSQDISINGNSKKEEPksETLE 885
Cdd:COG5116 778 SVRKVNTAVLSTTIKAAARAKQKPKE---KGPNDKEIKIESPSVEtEGERCTIKQREEKGIDAPAILNVKKKKP--YKVD 852

                       890       900       910       920       930       940       950
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19113173 886 NFTRVVPAQLPYISFNLNGRYYPVRKFTGGVLMLIDRESDKAPDLIELNRDAVPASAdtePGEQEASPPEDFEYPFD 962
Cdd:COG5116 853 NMTRILPQQSRYISFIKDDRFVPVRKFKGGVVVLRDREPKEPVALIETVRQMKDVNA---PLPTPFKVDDNVDFPSA 926
RPN2_C pfam18004
26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found ...
 769-931 3.09e-63

26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found in S. cerevisiae Rpn2 (26S proteasome regulatory subunit RPN2) as well as other eukaryotic species. A study revealed that the C-terminal 52 residues of the Rpn2 C-terminal domain are responsible for mediating interactions with the ubiquitin-binding subunit Rpn13. Futhermore, the extreme C-terminal 20 or 21 residues of Rpn2 (926-945 or 925-945) of S. cerevisiae, were shown to be equally effective at binding Rpn13. Multiple sequence alignments indicate that Rpn2 orthologs are highly conserved in this C-terminal region and share characteriztic acidic, aromatic, and proline residues, suggesting a common function. In the structure of Rpn2 from S. cerevisiae, this region is exposed and disordered, and is thus accessible for associating with Rpn13. The Rpn2 binding surface of human Rpn13 has been mapped by nuclear magnetic resonance titration to one surface of its Pru domain.


Pssm-ID: 465613 [Multi-domain]  Cd Length: 159  Bit Score: 210.89  E-value: 3.09e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   769 TALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKAsKGSNDDEMKIDKK 848
Cdd:pfam18004   1 TAIIGLNKDLKMPKFEFNSNAKPSLFAYPPETEEKKEKAPEKVATAVLSTTAKAKARAKKKEKEKE-KEKEEDKMDVDEE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   849 tTEEKEATPMEMDEEKSQDISingnSKKE-EPKSETLENFTRVVPAQLPYISFNLNGRYYPVRK-FTGGVLMLIDRESDK 926
Cdd:pfam18004  80 -KEEKEKKEKEKEEKEKKAEK----KKKEkEPTFYKLENPSRVLPAQLKYISFIKDSRYVPVKKkGTGGIIVLKDTKPDE 154


                  ....*
gi 19113173   927 APDLI 931
Cdd:pfam18004 155 PEELI 159
 
Name Accession Description Interval E-value
RPN2 COG5116
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 10-962 0e+00

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227446 [Multi-domain]  Cd Length: 926  Bit Score: 1528.73  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  10 SIITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAALVVSKVYYYLGEYDEALL 89
Cdd:COG5116   1 MSMTTARILPALLAELRDGRESEALDVINAHVDQLWPEISDDLRYIEALYDDDSFDPREMAALCLSKVYYVLGEYQQAIE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173  90 FALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKADPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLD 169
Cdd:COG5116  81 YALRAGDRFLVDDGSFYYETIVYKSIEMYVHMMDSAYIGGDKDIIDRILDFVLEVIGAKCVDDSEIGYLLGIAAEGLRLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 170 IIEYILNADKETDLKPYVLELAMTVVLDIEFRNRLLRLLLSSFLTETEPDYFSVGKCVVHLNDASVAAKLLMKLSSQNDD 249
Cdd:COG5116 161 IIEKYLSDGNDCDIINYLLDLAITLVEEEGFRKEILRMLAEIGPGKPKPDYFYVIKAVVYLNDAEKAKALIEKLVKENDL 240

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 250 KslLTAyQLAFDLEDSAPQEFLnsvmDLLPSPSVANSEEDAnadskkedsspcgyIIRILSGEQTVKYDREFLYAHNNTD 329
Cdd:COG5116 241 L--LYA-QVAFDLEDSASQEIL----EILVTELVAQGYDQA--------------VMSILSGEFTKKYLGAFLLEKNNTD 299

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 330 MLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYL 409
Cdd:COG5116 300 FKFLNSSKSSLARKFSRFHYAVSLANSFMNLGTSNDSFYRNNLDWLGKASNWAKFTATASLGVIHLGNSNPGYEILKPYL 379

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 410 PEEDApsSSTYSEGGAFYAMGLIHANHGRGVTEYLREQLKHTEDE---IVQYGLLLGIGLTGMASRDETLYESVKTILFN 486
Cdd:COG5116 380 PSEVA--SSRQKEGGALYALGLIKAGFGREDTEYLLEYFLDTEDEltpELAYGVCLGIGLINMGSANREIYEKLKELLKN 457

                       490       500       510       520       530       540       550       560
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 487 DNAVAGSAAGISMGLIMLGTASSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGM 566
Cdd:COG5116 458 DRALLGEAAVYGMGLLMLGTWSVEAIEDMRTYAGETQHERIKRGLGIGFALILYGRQEMADDYINELLYDKDSILRYNGV 537

                       570       580       590       600       610       620       630       640
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 567 FATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVELLVDSYNPHVRYGSAIALGIACANS 646
Cdd:COG5116 538 FSLALAYVGTGNLGVVSTLLHYAVSDGNDDVRRAAVIALGFVCCDDRDLLVGTVELLSESHNFHVRAGVAVALGIACAGT 617

                       650       660       670       680       690       700       710       720
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 647 GSNAALDLLSRLVEDATDFVRQGAMIAQAMILTQHNDQLNSKVSGIRKHFEQVINEKHEDALAKLGATLAQGIIDAGGRN 726
Cdd:COG5116 618 GDKVATDILEALMYDTNDFVRQSAMIAVGMILMQCNPELNPNVKRIIKKFNRVIVDKHESGLAKLGAVLAQGISEAGGRN 697

                       730       740       750       760       770       780       790       800
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 727 VTIALQTATGSLKLSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAK 806
Cdd:COG5116 698 VTISLRNATGILSADRIVGLVLFLQYWYWFPLIHFVSLSFLPTTVIGIRGSQAIPKFCFNEDLEEEEFEYPRMYEEASGK 777

                       810       820       830       840       850       860       870       880
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 807 TVQKVETAVLSTTVKAQARAKRAEREkasKGSNDDEMKIDKKTTE-EKEATPMEMDEEKSQDISINGNSKKEEPksETLE 885
Cdd:COG5116 778 SVRKVNTAVLSTTIKAAARAKQKPKE---KGPNDKEIKIESPSVEtEGERCTIKQREEKGIDAPAILNVKKKKP--YKVD 852

                       890       900       910       920       930       940       950
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19113173 886 NFTRVVPAQLPYISFNLNGRYYPVRKFTGGVLMLIDRESDKAPDLIELNRDAVPASAdtePGEQEASPPEDFEYPFD 962
Cdd:COG5116 853 NMTRILPQQSRYISFIKDDRFVPVRKFKGGVVVLRDREPKEPVALIETVRQMKDVNA---PLPTPFKVDDNVDFPSA 926
RPN2_C pfam18004
26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found ...
 769-931 3.09e-63

26S proteasome regulatory subunit RPN2 C-terminal domain; This is the C-terminal domain found in S. cerevisiae Rpn2 (26S proteasome regulatory subunit RPN2) as well as other eukaryotic species. A study revealed that the C-terminal 52 residues of the Rpn2 C-terminal domain are responsible for mediating interactions with the ubiquitin-binding subunit Rpn13. Futhermore, the extreme C-terminal 20 or 21 residues of Rpn2 (926-945 or 925-945) of S. cerevisiae, were shown to be equally effective at binding Rpn13. Multiple sequence alignments indicate that Rpn2 orthologs are highly conserved in this C-terminal region and share characteriztic acidic, aromatic, and proline residues, suggesting a common function. In the structure of Rpn2 from S. cerevisiae, this region is exposed and disordered, and is thus accessible for associating with Rpn13. The Rpn2 binding surface of human Rpn13 has been mapped by nuclear magnetic resonance titration to one surface of its Pru domain.


Pssm-ID: 465613 [Multi-domain]  Cd Length: 159  Bit Score: 210.89  E-value: 3.09e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   769 TALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKAsKGSNDDEMKIDKK 848
Cdd:pfam18004   1 TAIIGLNKDLKMPKFEFNSNAKPSLFAYPPETEEKKEKAPEKVATAVLSTTAKAKARAKKKEKEKE-KEKEEDKMDVDEE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   849 tTEEKEATPMEMDEEKSQDISingnSKKE-EPKSETLENFTRVVPAQLPYISFNLNGRYYPVRK-FTGGVLMLIDRESDK 926
Cdd:pfam18004  80 -KEEKEKKEKEKEEKEKKAEK----KKKEkEPTFYKLENPSRVLPAQLKYISFIKDSRYVPVKKkGTGGIIVLKDTKPDE 154


                  ....*
gi 19113173   927 APDLI 931
Cdd:pfam18004 155 PEELI 159
RPN1 COG5110
26S proteasome regulatory complex component [Posttranslational modification, protein turnover, ...
 350-856 2.56e-17

26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227441 [Multi-domain]  Cd Length: 881  Bit Score: 87.29  E-value: 2.56e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 350 AVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYLPeedapSSSTYSEGGAFYAM 429
Cdd:COG5110 363 AMGFVNDPINLGYENDSLIPLDDEWIYKCKVPGLISAFASIGVIESWNSDKGLETLDKYLY-----ADESYRKAGALLGI 437

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 430 GLIHA---NHGRGVTEYLREQLKHTEDEIVQYGLLLGIGLTGMASRDETLyESVKTILFNDNAVAGSA--AGISMGLIML 504
Cdd:COG5110 438 GLSGLrvfEERPPALALLSNYLQSSSSKHVIAAILGLGAAFSGTQAEEVL-ELLQPIMFSTDSPIEVVffASLTLGSVFV 516

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 505 GTA----SSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGMFATALAYCGTSNSK 580
Cdd:COG5110 517 GTCngdlTSLILQTFVERGKIESETQWFRFLALGLASLFYGRKDQVDDVEETIMAIEGALSKHEEILVKGCQYVGTGDVL 596

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 581 IVRDVLHISVSDVNDDVRRA------------AVCALGFICFKDPNALISTVELLVDsyNPHVRYGSAIALGIACANSGS 648
Cdd:COG5110 597 VIQSLLHVKDEFTGDTLKNEealieslallgcALIAMGEDIGSEMVLRHFSHSMHYG--SSHIRSVLPLAYGILSPSNPQ 674

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 649 NAALDLLSRLVEDATDFVRQGAMIAQAMIltqHNDQLNSKVSGIrkhFEQVINEKHEDALAKLGATLAQGIIDAGGRNVT 728
Cdd:COG5110 675 MNVFDTLERSSHDGDLNVIINTIFAMGLI---GAGTLNARLAQL---LRQLASYYYKESKALFVLRIAQGLLSLGKGTMT 748

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 729 IA---------LQTATGSLkLSAIVGL----TVFLQYWYWfpLTHFMSLSFSPTALIGLDKNLNAPKFsfisNVRpklFA 795
Cdd:COG5110 749 ISplyfdkttlMPKNTAGL-FTTVFMLldssIFPLVSSHA--LMYFLLCQIRPQKYVTLSEKGEPIKV----NVR---VG 818

                       490       500       510       520       530       540
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 19113173 796 YPPKSVQPTA--KTVQKVETAVLSTTVKAQARAKRAEREKASKGSNDDEMKIDKKTTEEKEAT 856
Cdd:COG5110 819 QAVNTVGQAGrpKKITGWQTHTTPVLLSHKERAELDTDEYNVCTSYIEGVVILKKNPDYREEE 881
HEAT COG1413
HEAT repeat [General function prediction only];
542-673 1.26e-08

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 54.63  E-value: 1.26e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 542 RQQEADGIIKELNNDLDPTLRYAGmfATALAYCGtsnSKIVRDVLHISVSDVNDDVRRAAVCALGFIcfKDPNALISTVE 621
Cdd:COG1413  13 GDPAAVPALIAALADEDPDVRAAA--ARALGRLG---DPRAVPALLEALKDPDPEVRAAAAEALGRI--GDPEAVPALIA 85

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 19113173 622 LLVDSyNPHVRYGSAIALGiacaNSGSNAALDLLSRLVEDATDFVRQGAMIA 673
Cdd:COG1413  86 ALKDE-DPEVRRAAAEALG----RLGDPAAVPALLEALKDPDWEVRRAAARA 132
HEAT_2 pfam13646
HEAT repeats; This family includes multiple HEAT repeats.
 582-676 3.58e-08

HEAT repeats; This family includes multiple HEAT repeats.


Pssm-ID: 433376 [Multi-domain]  Cd Length: 88  Bit Score: 51.57  E-value: 3.58e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   582 VRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALIstVELLVDSyNPHVRYGSAIALGIacanSGSNAALDLLSRLVED 661
Cdd:pfam13646   1 LPALLQALLRDPDPEVRAAAIRALGRIGDPEAVPAL--LELLKDE-DPAVRRAAAEALGK----IGDPEALPALLELLRD 73

                          90
                  ....*....|....*
gi 19113173   662 ATDFVRQGAmIAQAM 676
Cdd:pfam13646  74 DDDDVVRAA-AAEAL 87
HEAT COG1413
HEAT repeat [General function prediction only];
568-673 1.14e-06

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 48.86  E-value: 1.14e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 568 ATALAYCGTSNS-KIVRDVLHisvsDVNDDVRRAAVCALGFIcfKDPNALISTVELLVDSyNPHVRYGSAIALGiacaNS 646
Cdd:COG1413   6 ARALGRLGDPAAvPALIAALA----DEDPDVRAAAARALGRL--GDPRAVPALLEALKDP-DPEVRAAAAEALG----RI 74

                        90       100
                ....*....|....*....|....*..
gi 19113173 647 GSNAALDLLSRLVEDATDFVRQGAMIA 673
Cdd:COG1413  75 GDPEAVPALIAALKDEDPEVRRAAAEA 101
HEAT_2 pfam13646
HEAT repeats; This family includes multiple HEAT repeats.
 546-640 1.14e-06

HEAT repeats; This family includes multiple HEAT repeats.


Pssm-ID: 433376 [Multi-domain]  Cd Length: 88  Bit Score: 47.33  E-value: 1.14e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173   546 ADGIIKELNNDLDPTLRYAgmFATALAYCGTSNskiVRDVLHISVSDVNDDVRRAAVCALGFIcfKDPNALISTVELLVD 625
Cdd:pfam13646   1 LPALLQALLRDPDPEVRAA--AIRALGRIGDPE---AVPALLELLKDEDPAVRRAAAEALGKI--GDPEALPALLELLRD 73

                          90
                  ....*....|....*
gi 19113173   626 SYNPHVRYGSAIALG 640
Cdd:pfam13646  74 DDDDVVRAAAAEALA 88
PC_rep pfam01851
Proteasome/cyclosome repeat;
424-457 3.80e-05

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 41.64  E-value: 3.80e-05
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 19113173   424 GAFYAMGLIHANHGRG-VTEYLREQLKHTEDEIVQ 457
Cdd:pfam01851   1 GAALALGLIHAGSGNEeALDLLLPYLSDTSAESRA 35
PC_rep pfam01851
Proteasome/cyclosome repeat;
494-528 4.31e-05

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 41.25  E-value: 4.31e-05
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 19113173   494 AAGISMGLIMLGTASSAAIDEMLQYAHETQHEKII 528
Cdd:pfam01851   1 GAALALGLIHAGSGNEEALDLLLPYLSDTSAESRA 35
HEAT COG1413
HEAT repeat [General function prediction only];
536-640 1.10e-04

HEAT repeat [General function prediction only];


Pssm-ID: 441023 [Multi-domain]  Cd Length: 137  Bit Score: 43.08  E-value: 1.10e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19113173 536 ALIVYGRQQEADGIIKELNNDlDPTLRYAGmfATALAYCGTSNskiVRDVLHISVSDVNDDVRRAAVCALGFIcfKDPNA 615
Cdd:COG1413  39 ALGRLGDPRAVPALLEALKDP-DPEVRAAA--AEALGRIGDPE---AVPALIAALKDEDPEVRRAAAEALGRL--GDPAA 110

                        90       100
                ....*....|....*....|....*
gi 19113173 616 LISTVELLVDSyNPHVRYGSAIALG 640
Cdd:COG1413 111 VPALLEALKDP-DWEVRRAAARALG 134
PC_rep pfam01851
Proteasome/cyclosome repeat;
634-668 1.85e-04

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 39.33  E-value: 1.85e-04
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 19113173   634 GSAIALGIACANSGSNAALDLLSRLVEDATDFVRQ 668
Cdd:pfam01851   1 GAALALGLIHAGSGNEEALDLLLPYLSDTSAESRA 35
HEAT_EZ pfam13513
HEAT-like repeat; The HEAT repeat family is related to armadillo/beta-catenin-like repeats ...
 597-640 5.48e-03

HEAT-like repeat; The HEAT repeat family is related to armadillo/beta-catenin-like repeats (see pfam00514). These EZ repeats are found in subunits of cyanobacterial phycocyanin lyase and other proteins and probably carry out a scaffolding role.


Pssm-ID: 463906 [Multi-domain]  Cd Length: 55  Bit Score: 35.81  E-value: 5.48e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 19113173   597 VRRAAVCALGFICFKDPNALISTVELLVD-------SYNPHVRYGSAIALG 640
Cdd:pfam13513   3 VREAAALALGSLAEGGPDLLAPAVPELLPallpllnDDSDLVREAAAWALG 53
PC_rep pfam01851
Proteasome/cyclosome repeat;
386-418 6.37e-03

Proteasome/cyclosome repeat;


Pssm-ID: 460361 [Multi-domain]  Cd Length: 35  Bit Score: 35.09  E-value: 6.37e-03
                          10        20        30
                  ....*....|....*....|....*....|....
gi 19113173   386 ATAALGVIHRGYYNQ-AMNILRPYLPEEDAPSSS 418
Cdd:pfam01851   2 AALALGLIHAGSGNEeALDLLLPYLSDTSAESRA 35
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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