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Conserved domains on  [gi|24585022|ref|NP_609900|]
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uncharacterized protein Dmel_CG10343 [Drosophila melanogaster]

Protein Classification

RWD domain-containing protein( domain architecture ID 10651873)

RWD domain-containing protein such as RWD domain-containing protein 3 (RWDD3), also known as RSUME, a RWD-containing protein that enhances SUMO conjugation by interacting with the SUMO conjugase Ubc9, increases Ubc9 thioester formation and therefore favours sumoylation of specific targets

CATH:  3.10.110.10
Gene Ontology:  GO:0005515
PubMed:  26276859|25918163

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWD-RWDD4 cd23817
RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called ...
 10-112 9.54e-67

RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called protein FAM28A, is a target of the tumor suppressor MicroRNA (MiR)-506 in bladder cancer cells. Downregulation of RWDD4 suppresses bladder cancer cell proliferation, migration and invasion. RWDD4 has also been identified as a modifier of metastasis in human prostate cancer.


:

Pssm-ID: 467653  Cd Length: 104  Bit Score: 200.43  E-value: 9.54e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEGDTNFKEIDSVTFQYKYGEEDNYKSFLVELKWGENYPDEAPAINMNAFYNRNLLPAVKEGIQTALSTEA 89
Cdd:cd23817   2 EELEVLLSIYEGDENFKQISDTTFQYKYGEDGDPKSFLLEISWPENYPEEPPIINLDAFYNKHISSSVKEKIVSKLNEEA 81

                        90       100
                ....*....|....*....|...
gi 24585022  90 DQWLGCGMTYTLFECLKDNLEQL 112
Cdd:cd23817  82 EQNLGSAMTYTLFEWAKENAEEL 104
 
Name Accession Description Interval E-value
RWD-RWDD4 cd23817
RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called ...
 10-112 9.54e-67

RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called protein FAM28A, is a target of the tumor suppressor MicroRNA (MiR)-506 in bladder cancer cells. Downregulation of RWDD4 suppresses bladder cancer cell proliferation, migration and invasion. RWDD4 has also been identified as a modifier of metastasis in human prostate cancer.


Pssm-ID: 467653  Cd Length: 104  Bit Score: 200.43  E-value: 9.54e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEGDTNFKEIDSVTFQYKYGEEDNYKSFLVELKWGENYPDEAPAINMNAFYNRNLLPAVKEGIQTALSTEA 89
Cdd:cd23817   2 EELEVLLSIYEGDENFKQISDTTFQYKYGEDGDPKSFLLEISWPENYPEEPPIINLDAFYNKHISSSVKEKIVSKLNEEA 81

                        90       100
                ....*....|....*....|...
gi 24585022  90 DQWLGCGMTYTLFECLKDNLEQL 112
Cdd:cd23817  82 EQNLGSAMTYTLFEWAKENAEEL 104
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 11-112 1.15e-20

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 82.79  E-value: 1.15e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022     11 EREALQSIYEGDTNFKEIDSVTFQYKY-------GEEDNYKSFLVELKWGENYPDEAPAINMNAFynRNLLPAVKEGIQT 83
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIklspssdEGEDQYVSLTLQVKLPENYPDEAPPISLLNS--EGLSDEQLAELLK 78

                           90       100
                   ....*....|....*....|....*....
gi 24585022     84 ALSTEADQWLGCGMTYTLFECLKDNLEQL 112
Cdd:smart00591  79 KLEEIAEENLGEVMIFELVEKLQEFLSEF 107
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 6-109 2.23e-20

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 82.37  E-value: 2.23e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022     6 EQQTEEREALQSIYEGDTNFKEIDSVT---FQYKYGEE------DNYKSFLVELKWGENYPDEAPAINMNAFynRNLLPA 76
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDSPYEsleIEIKLSLDsdesdsSHLPPLVLKFTLPEDYPDEPPKISLSSP--WNLSDE 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 24585022    77 VKEGIQTALSTEADQWLGCGMTYTLFECLKDNL 109
Cdd:pfam05773  79 QVLSLLEELEELAEENLGEVMIFELIEWLQENL 111
 
Name Accession Description Interval E-value
RWD-RWDD4 cd23817
RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called ...
 10-112 9.54e-67

RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called protein FAM28A, is a target of the tumor suppressor MicroRNA (MiR)-506 in bladder cancer cells. Downregulation of RWDD4 suppresses bladder cancer cell proliferation, migration and invasion. RWDD4 has also been identified as a modifier of metastasis in human prostate cancer.


Pssm-ID: 467653  Cd Length: 104  Bit Score: 200.43  E-value: 9.54e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEGDTNFKEIDSVTFQYKYGEEDNYKSFLVELKWGENYPDEAPAINMNAFYNRNLLPAVKEGIQTALSTEA 89
Cdd:cd23817   2 EELEVLLSIYEGDENFKQISDTTFQYKYGEDGDPKSFLLEISWPENYPEEPPIINLDAFYNKHISSSVKEKIVSKLNEEA 81

                        90       100
                ....*....|....*....|...
gi 24585022  90 DQWLGCGMTYTLFECLKDNLEQL 112
Cdd:cd23817  82 EQNLGSAMTYTLFEWAKENAEEL 104
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 11-112 1.15e-20

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 82.79  E-value: 1.15e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022     11 EREALQSIYEGDTNFKEIDSVTFQYKY-------GEEDNYKSFLVELKWGENYPDEAPAINMNAFynRNLLPAVKEGIQT 83
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIklspssdEGEDQYVSLTLQVKLPENYPDEAPPISLLNS--EGLSDEQLAELLK 78

                           90       100
                   ....*....|....*....|....*....
gi 24585022     84 ALSTEADQWLGCGMTYTLFECLKDNLEQL 112
Cdd:smart00591  79 KLEEIAEENLGEVMIFELVEKLQEFLSEF 107
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 6-109 2.23e-20

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 82.37  E-value: 2.23e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022     6 EQQTEEREALQSIYEGDTNFKEIDSVT---FQYKYGEE------DNYKSFLVELKWGENYPDEAPAINMNAFynRNLLPA 76
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDSPYEsleIEIKLSLDsdesdsSHLPPLVLKFTLPEDYPDEPPKISLSSP--WNLSDE 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 24585022    77 VKEGIQTALSTEADQWLGCGMTYTLFECLKDNL 109
Cdd:pfam05773  79 QVLSLLEELEELAEENLGEVMIFELIEWLQENL 111
RWD_GCN2 cd23823
RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called ...
 6-112 2.59e-12

RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called eukaryotic translation initiation factor 2-alpha kinase 4 (EIF2AK4), acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. It also plays a role in modulating the adaptive immune response to yellow fever virus infection and promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation.


Pssm-ID: 467659  Cd Length: 117  Bit Score: 61.08  E-value: 2.59e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022   6 EQQTEEREALQSIYEGDtnFKEIDSVTFQYKYGE------------EDNYKSFLVELKWGENYPDEAPAINMNAfyNRNL 73
Cdd:cd23823   2 EEQEEELEALQSIYGDD--FEDLSSKKAVWSPPEfrirlrpqegesEENHVSVDLHVKFPPTYPDVPPEIELEN--VKGL 77

                        90       100       110
                ....*....|....*....|....*....|....*....
gi 24585022  74 LPAVKEGIQTALSTEADQWLGCGMTYTLFECLKDNLEQL 112
Cdd:cd23823  78 SDEQLEELLKELEELAKELLGEEMIFELAEAVQEFLEEH 116
RWD_RWDD1 cd23816
RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called ...
 5-112 8.78e-08

RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called DRG family-regulatory protein 2 (DFRP2), or PTD013, interacts with DRG2 and protects DRG2 from proteolytic degradation. It is an androgen receptor-interacting protein that functions as a coactivator of androgen-dependent transcription.


Pssm-ID: 467652  Cd Length: 118  Bit Score: 48.83  E-value: 8.78e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022   5 LEQQTEEREALQSIY-EGDTNFKEIDSVTFQYKY---GEEDNYKSFLVELK--WGENYPDEAPAINMNAfyNRNLLPAVK 78
Cdd:cd23816   2 KEEQRNELEALESIYpDEFTVLSEEPPISFTITVtseEEENEDETVSVTLKftYTEKYPDEAPLIEIIS--HENLEDEDI 79

                        90       100       110
                ....*....|....*....|....*....|....
gi 24585022  79 EGIQTALSTEADQWLGCGMTYTLFECLKDNLEQL 112
Cdd:cd23816  80 EDLLELLEQQAEENLGMVMVFTIVSAVQEKLNEI 113
RWD_RNF25 cd23818
RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also ...
 10-109 3.24e-07

RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also known as AO7, is a putative E3 ubiquitin-protein ligase that was initially identified as an interacting protein of the E2 ubiquitin-conjugating enzyme, Ubc5B. It is ubiquitously expressed in various tissues and is predominantly localized in the nucleus. RNF25 activates nuclear factor (NF)-kappaB-dependent gene expression upon stimulation with interleukin-1 beta (IL-1beta), or tumor necrosis factor (TNF), or overexpression of NF-kappaB-inducing kinase. It interacts with the p65 transactivation domain (TAD) and modulates its transcriptional activity.


Pssm-ID: 467654  Cd Length: 109  Bit Score: 47.15  E-value: 3.24e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEGD----TNFKEIDSVTFQYKYG----EEDNYKSFLVELKWGENYPDEAPAINM-NAfynRNLLPAVKEG 80
Cdd:cd23818   3 EELEALEAIYPDElkvvSEDGAPTELSITLHPAtaddESEQYVRLTLVITLPPGYPEEPPKISLrNP---RGLSDARLAR 79

                        90       100
                ....*....|....*....|....*....
gi 24585022  81 IQTALSTEADQWLGCGMTYTLFECLKDNL 109
Cdd:cd23818  80 LLSLLKELAEERAGEPMLFELIELAKEFL 108
RWD_YLR419W-like cd23827
RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related ...
 10-110 4.97e-07

RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related proteins; YLR419W (EC 3.6.4.13) may act as an ATP-binding RNA helicase. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467662  Cd Length: 104  Bit Score: 46.47  E-value: 4.97e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEgdTNFKEIDSVTFQYKYGEEDNYKSFL-VELKWGENYPDEAPAINMNAFynrNLLPA--VKEGIQTALS 86
Cdd:cd23827   3 EEIEALEAIYG--EKFEVISDDSCEITLNSPTKTKPSLkLKFYKSSSYPNSLPGIFISSS---DKLPAyiKLAIIRQLLQ 77

                        90       100
                ....*....|....*....|....
gi 24585022  87 TEADQWLGCGMTYTLFECLKDNLE 110
Cdd:cd23827  78 YARDNLLGDPMIFSIVEWLEENIE 101
RWD_DRWD_ELF-like cd11605
RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF ...
 14-103 1.92e-05

RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF domains. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. The RWD domain (named after three major RWD-containing proteins: RING finger, WD-repeat-containing proteins and DEXD-like helicases) mediates protein-protein interactions in a variety of pathways in eukaryotes. The DRWD domain is responsible for substrate binding. It is involved in interactions with other kinetochore proteins. The ELF (N-terminal E2-like fold) domain is found in all Fanconi anemia group L protein (FANCL) homologs. It is required to promote efficient DNA damage-induced FANCD2 (Fanconi anemia group D2 protein) monoubiquitination in vertebrate cells.


Pssm-ID: 467641  Cd Length: 94  Bit Score: 41.78  E-value: 1.92e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  14 ALQSIYEGDtnFKEIDS---VTFQY--KYGEEDNYKSFLVELKWGENY-PDEAPAINMNAFynRNLLPAVKEGIQTALST 87
Cdd:cd11605   1 ALESIYGDE--LEVLSDdspLRFSIrlSPEEEEDDPPLELEFTLPPGYpPEEPPLITLRSP--KLSSAERLSLLKLELEE 76

                        90
                ....*....|....*.
gi 24585022  88 EADQWLGCGMTYTLFE 103
Cdd:cd11605  77 AAEENLGEPMLFDLVE 92
RWD_RWDD3 cd23819
RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called ...
 10-109 6.01e-03

RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called RWD domain-containing sumoylation enhancer (RSUME), acts as an enhancer of SUMO conjugation and has no effect on ubiquitination. It increases protein sumoylation (a dynamic ubiquitin-like post translational modification) of several proteins including HIF1alpha and I-kappa-B, through direct interaction with UBC9. Its RWD domain is required for the sumoylation enhancement activity.


Pssm-ID: 467655  Cd Length: 106  Bit Score: 35.38  E-value: 6.01e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24585022  10 EEREALQSIYEGDTNF------KEIDSVTFQYKYGE----EDNYKSFLVELKwgENYPDEAPAINMNAfynRNLLPAVKE 79
Cdd:cd23819   1 DELSVLQAIFCGPGEFevlsssETSDGVSFKIQISVegfdEDIVLKLTFHLS--PNYPSSLPDISVSS---EQLTRAQCN 75

                        90       100       110
                ....*....|....*....|....*....|
gi 24585022  80 GIQTALSTEADQWLGCGMTYTLFECLKDNL 109
Cdd:cd23819  76 DLQDSLLEYANSLLGEPMVLELVLWLQENL 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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