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Conserved domains on  [gi|21355709|ref|NP_651072|]
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mitochondrial ribosomal protein L45 [Drosophila melanogaster]

Protein Classification

Tim44 domain-containing protein( domain architecture ID 10659503)

Tim44 domain-containing protein similar to mitochondrial 39S ribosomal protein L45 and to mitochondrial import inner membrane translocase subunit Tim44, an essential component of the eukaryotic PAM complex which is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Gene Ontology:  GO:0003735|GO:0032543

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Tim44 smart00978
Tim44 is an essential component of the machinery that mediates the translocation of ...
 151-295 1.76e-39

Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane; Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region.


:

Pssm-ID: 214950  Cd Length: 147  Bit Score: 137.07  E-value: 1.76e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709    151 MAVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDKTIRWKFLQSLEPPRVVHARV 230
Cdd:smart00978   2 KALREIKRIDPGFDTERFLEGAKDAFPMIQEAWDRGDLETLRELLTPEMYNELAAQIAEREARGLFVENKLLDIDVVVLD 81

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 21355709    231 TEVITK--ENQFAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHIsNEYGKWRLHDK 295
Cdd:smart00978  82 AALLEAwqEGDGAVITVRFHGQQIDVTRDAKGGVVVGGEDKPKEFTEVWTFTRDL-NGDPNWRLSGI 147
 
Name Accession Description Interval E-value
Tim44 smart00978
Tim44 is an essential component of the machinery that mediates the translocation of ...
 151-295 1.76e-39

Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane; Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region.


Pssm-ID: 214950  Cd Length: 147  Bit Score: 137.07  E-value: 1.76e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709    151 MAVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDKTIRWKFLQSLEPPRVVHARV 230
Cdd:smart00978   2 KALREIKRIDPGFDTERFLEGAKDAFPMIQEAWDRGDLETLRELLTPEMYNELAAQIAEREARGLFVENKLLDIDVVVLD 81

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 21355709    231 TEVITK--ENQFAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHIsNEYGKWRLHDK 295
Cdd:smart00978  82 AALLEAwqEGDGAVITVRFHGQQIDVTRDAKGGVVVGGEDKPKEFTEVWTFTRDL-NGDPNWRLSGI 147
Tim44 pfam04280
Tim44-like domain; Tim44 is an essential component of the machinery that mediates the ...
 152-293 7.78e-31

Tim44-like domain; Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane. Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region. This family includes the C-terminal region of Tim44 that has been shown to form a stable proteolytic fragment in yeast. This region is also found in a set of smaller bacterial proteins. The molecular function of the bacterial members of this family is unknown but transport seems likely. The crystal structure of the C terminal of Tim44 has revealed a large hydrophobic pocket which might play an important role in interacting with the acyl chains of lipid molecules in the mitochondrial membrane.


Pssm-ID: 427835  Cd Length: 145  Bit Score: 114.25  E-value: 7.78e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709   152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDKtIRWKFLQSLEPPRVVHARVT 231
Cdd:pfam04280   3 ALRAIKAIDPSFDVEEFLEGAKEAFEPILEAYAKGDLETLRPLLTEEVFEEFAAAIDER-EAQGLTNDLEIVDIREVDIA 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 21355709   232 EVITKENQfAQVTVRFHSQQMLAIYDRF-GRLMHGSEIITKDVLEYVVFEKHISNEYGKWRLH 293
Cdd:pfam04280  82 EAKMEGGT-AVITVRFRAQQIDVTRDDAtGEVVEGDPDDPVEVTEVWTFTRDLGNPDPNWRLV 143
Tim44 COG4395
Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];
152-292 5.43e-09

Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];


Pssm-ID: 443521  Cd Length: 186  Bit Score: 55.30  E-value: 5.43e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709 152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSercyPEMMHNVKD---------KTIRWKFLqslep 222
Cdd:COG4395  41 GLAAIAAAPPGFDVEAFLRGAKAAFEMIQAAWDAGDLEDLRELLT----PEVYAEFQAaiaereaagETNETTFV----- 111

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709 223 pRVVHARVTEVITKENQfAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHISNEYGKWRL 292
Cdd:COG4395 112 -GILDAELVDAELEGDE-AEVTVRFVGELREATRDADGEVVEGDPAAAEEFREVWTFARPLGSGDPNWLL 179
Tim44_TimA_adap NF033779
Tim44/TimA family putative adaptor protein; Members of this family resemble both the ...
 152-292 3.00e-03

Tim44/TimA family putative adaptor protein; Members of this family resemble both the eukaryotic protein Tim44, important to the assembly of a protein translocase in mitochondria, and the TimA protein of alpha-proteobacteria such as Caulobacter crescentus. TimA may assist in protein recruitment to the membrane, as Tim44, but appears not to be part of any complex associated with protein translocation.


Pssm-ID: 468184  Cd Length: 215  Bit Score: 38.71  E-value: 3.00e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709  152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDK-----TIRWKFLqSLEPPRVV 226
Cdd:NF033779  74 GLAAIKAADPSFDPKEFLEGARAAYEMIVMAFAKGDRDTLKPLLSKDVYESFEAAIAAReaageTVEAEFV-GIDKAEIV 152

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 21355709  227 HARVtevitkENQFAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHISNEYGKWRL 292
Cdd:NF033779 153 DAEL------EGRVAEITVRFVSELISVTRDKDGEVVDGSPDAVKEVKDVWTFARDLGSRDPNWKL 212
 
Name Accession Description Interval E-value
Tim44 smart00978
Tim44 is an essential component of the machinery that mediates the translocation of ...
 151-295 1.76e-39

Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane; Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region.


Pssm-ID: 214950  Cd Length: 147  Bit Score: 137.07  E-value: 1.76e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709    151 MAVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDKTIRWKFLQSLEPPRVVHARV 230
Cdd:smart00978   2 KALREIKRIDPGFDTERFLEGAKDAFPMIQEAWDRGDLETLRELLTPEMYNELAAQIAEREARGLFVENKLLDIDVVVLD 81

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 21355709    231 TEVITK--ENQFAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHIsNEYGKWRLHDK 295
Cdd:smart00978  82 AALLEAwqEGDGAVITVRFHGQQIDVTRDAKGGVVVGGEDKPKEFTEVWTFTRDL-NGDPNWRLSGI 147
Tim44 pfam04280
Tim44-like domain; Tim44 is an essential component of the machinery that mediates the ...
 152-293 7.78e-31

Tim44-like domain; Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane. Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region. This family includes the C-terminal region of Tim44 that has been shown to form a stable proteolytic fragment in yeast. This region is also found in a set of smaller bacterial proteins. The molecular function of the bacterial members of this family is unknown but transport seems likely. The crystal structure of the C terminal of Tim44 has revealed a large hydrophobic pocket which might play an important role in interacting with the acyl chains of lipid molecules in the mitochondrial membrane.


Pssm-ID: 427835  Cd Length: 145  Bit Score: 114.25  E-value: 7.78e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709   152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDKtIRWKFLQSLEPPRVVHARVT 231
Cdd:pfam04280   3 ALRAIKAIDPSFDVEEFLEGAKEAFEPILEAYAKGDLETLRPLLTEEVFEEFAAAIDER-EAQGLTNDLEIVDIREVDIA 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 21355709   232 EVITKENQfAQVTVRFHSQQMLAIYDRF-GRLMHGSEIITKDVLEYVVFEKHISNEYGKWRLH 293
Cdd:pfam04280  82 EAKMEGGT-AVITVRFRAQQIDVTRDDAtGEVVEGDPDDPVEVTEVWTFTRDLGNPDPNWRLV 143
Tim44 COG4395
Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];
152-292 5.43e-09

Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];


Pssm-ID: 443521  Cd Length: 186  Bit Score: 55.30  E-value: 5.43e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709 152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSercyPEMMHNVKD---------KTIRWKFLqslep 222
Cdd:COG4395  41 GLAAIAAAPPGFDVEAFLRGAKAAFEMIQAAWDAGDLEDLRELLT----PEVYAEFQAaiaereaagETNETTFV----- 111

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709 223 pRVVHARVTEVITKENQfAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHISNEYGKWRL 292
Cdd:COG4395 112 -GILDAELVDAELEGDE-AEVTVRFVGELREATRDADGEVVEGDPAAAEEFREVWTFARPLGSGDPNWLL 179
Tim44_TimA_adap NF033779
Tim44/TimA family putative adaptor protein; Members of this family resemble both the ...
 152-292 3.00e-03

Tim44/TimA family putative adaptor protein; Members of this family resemble both the eukaryotic protein Tim44, important to the assembly of a protein translocase in mitochondria, and the TimA protein of alpha-proteobacteria such as Caulobacter crescentus. TimA may assist in protein recruitment to the membrane, as Tim44, but appears not to be part of any complex associated with protein translocation.


Pssm-ID: 468184  Cd Length: 215  Bit Score: 38.71  E-value: 3.00e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355709  152 AVRKIRSYDENFSSDDFGAEAQDIYIQAHTHMAAKDKYKIREFVSERCYPEMMHNVKDK-----TIRWKFLqSLEPPRVV 226
Cdd:NF033779  74 GLAAIKAADPSFDPKEFLEGARAAYEMIVMAFAKGDRDTLKPLLSKDVYESFEAAIAAReaageTVEAEFV-GIDKAEIV 152

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 21355709  227 HARVtevitkENQFAQVTVRFHSQQMLAIYDRFGRLMHGSEIITKDVLEYVVFEKHISNEYGKWRL 292
Cdd:NF033779 153 DAEL------EGRVAEITVRFVSELISVTRDKDGEVVDGSPDAVKEVKDVWTFARDLGSRDPNWKL 212
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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