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Conserved domains on  [gi|25151414|ref|NP_741764|]
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Tubulin-specific chaperone cofactor E-like protein [Caenorhabditis elegans]

Protein Classification

leucine-rich repeat domain-containing protein; ubiquitin family protein( domain architecture ID 1909116)

leucine-rich repeat (LRR) domain-containing protein may participate in protein-protein interactions| ubiquitin family protein belongs to a diverse class of protein modifier and gene expression regulatory proteins that participate in a number of cellular processes; has an N-terminal domain with similarity to the N-terminus of ubiquitin fusion degradation UFD1 which functions at a post-ubiquitation step in the ubiquitin fusion degradation (UFD) pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ubl1_cv_Nsp3_N-like super family cl28922
first ubiquitin-like (Ubl) domain located at the N-terminus of coronavirus SARS-CoV ...
 327-414 3.46e-08

first ubiquitin-like (Ubl) domain located at the N-terminus of coronavirus SARS-CoV non-structural protein 3 (Nsp3) and related proteins; This ubiquitin-like (Ubl) domain (Ubl1) is found at the N-terminus of coronavirus Nsp3, a large multi-functional multi-domain protein which is an essential component of the replication/transcription complex (RTC). The functions of Ubl1 in CoVs are related to single-stranded RNA (ssRNA) binding and to interacting with the nucleocapsid (N) protein. SARS-CoV Ubl1 has been shown to bind ssRNA having AUA patterns, and since the 5'-UTR of the SARS-CoV genome has a number of AUA repeats, it may bind there. In mouse hepatitis virus (MHV), this Ubl1 domain binds the cognate N protein. Adjacent to Ubl1 is a Glu-rich acidic region (also referred to as hypervariable region, HVR); Ubl1 together with HVR has been called Nsp3a. Currently, the function of HVR in CoVs is unknown. This model corresponds to one of two Ubl domains in Nsp3; the other is located N-terminal to the papain-like protease (PLpro) and is not represented by this model.


The actual alignment was detected with superfamily member cd17045:

Pssm-ID: 475130  Cd Length: 87  Bit Score: 50.71  E-value: 3.46e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 327 DLTPKKEAVVKILCEEKEvnQEITISLEPTVLDFMKILDPKVGVKFTRMKLFLLREDGR---TDDFSSSDYNmpLHYFKI 403
Cdd:cd17045   1 DLTPPKSAKVTVHFEDQV--ESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEMSifgPEELRFPSRA--LYSYNI 76

                        90
                ....*....|.
gi 25151414 404 EDGDSFLVQEK 414
Cdd:cd17045  77 RDGDEILVEPK 87
LRR super family cl34836
Leucine-rich repeat (LRR) protein [Transcription];
47-288 7.57e-06

Leucine-rich repeat (LRR) protein [Transcription];


The actual alignment was detected with superfamily member COG4886:

Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 48.01  E-value: 7.57e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414  47 ELLVLNNMNIDTIGDSEKLATLashvseaDLGWNQISkwsDIACILKNLPHLRVLNIGHNPLNPvIDHELPVST-LHTII 125
Cdd:COG4886  97 NLTELDLSGNEELSNLTNLESL-------DLSGNQLT---DLPEELANLTNLKELDLSNNQLTD-LPEPLGNLTnLKSLD 165

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 126 LNGTHLpfKTLQSFLSVLPKVTELHMSDNQFNDDddcDEPIS--TTVRTVHLNRCGFlkwSSVMNVVKRFPNVCSVFVCE 203
Cdd:COG4886 166 LSNNQL--TDLPEELGNLTNLKELDLSNNQITDL---PEPLGnlTNLEELDLSGNQL---TDLPEPLANLTNLETLDLSN 237

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 204 NPLKDVthcKHFEQLPFWNFLNLAKTSIDSWDSLDQLNRMTS-------ISDLRVPNIPLLDALTNEERLHLIIGRLHHL 276
Cdd:COG4886 238 NQLTDL---PELGNLTNLEELDLSNNQLTDLPPLANLTNLKTldlsnnqLTDLKLKELELLLGLNSLLLLLLLLNLLELL 314

                       250
                ....*....|..
gi 25151414 277 RVLNGSKISSEQ 288
Cdd:COG4886 315 ILLLLLTTLLLL 326
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 327-414 3.46e-08

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 50.71  E-value: 3.46e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 327 DLTPKKEAVVKILCEEKEvnQEITISLEPTVLDFMKILDPKVGVKFTRMKLFLLREDGR---TDDFSSSDYNmpLHYFKI 403
Cdd:cd17045   1 DLTPPKSAKVTVHFEDQV--ESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEMSifgPEELRFPSRA--LYSYNI 76

                        90
                ....*....|.
gi 25151414 404 EDGDSFLVQEK 414
Cdd:cd17045  77 RDGDEILVEPK 87
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
47-288 7.57e-06

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 48.01  E-value: 7.57e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414  47 ELLVLNNMNIDTIGDSEKLATLashvseaDLGWNQISkwsDIACILKNLPHLRVLNIGHNPLNPvIDHELPVST-LHTII 125
Cdd:COG4886  97 NLTELDLSGNEELSNLTNLESL-------DLSGNQLT---DLPEELANLTNLKELDLSNNQLTD-LPEPLGNLTnLKSLD 165

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 126 LNGTHLpfKTLQSFLSVLPKVTELHMSDNQFNDDddcDEPIS--TTVRTVHLNRCGFlkwSSVMNVVKRFPNVCSVFVCE 203
Cdd:COG4886 166 LSNNQL--TDLPEELGNLTNLKELDLSNNQITDL---PEPLGnlTNLEELDLSGNQL---TDLPEPLANLTNLETLDLSN 237

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 204 NPLKDVthcKHFEQLPFWNFLNLAKTSIDSWDSLDQLNRMTS-------ISDLRVPNIPLLDALTNEERLHLIIGRLHHL 276
Cdd:COG4886 238 NQLTDL---PELGNLTNLEELDLSNNQLTDLPPLANLTNLKTldlsnnqLTDLKLKELELLLGLNSLLLLLLLLNLLELL 314

                       250
                ....*....|..
gi 25151414 277 RVLNGSKISSEQ 288
Cdd:COG4886 315 ILLLLLTTLLLL 326
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 48-108 6.99e-03

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 37.84  E-value: 6.99e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 25151414  48 LLVLN--NMNIDTIGDsekLATLaSHVSEADLGWNQISKWSDIACILKNLPHLRVLNIGHNPL 108
Cdd:cd21340 122 LRVLNisGNNIDSLEP---LAPL-RNLEQLDASNNQISDLEELLDLLSSWPSLRELDLTGNPV 180
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 327-414 3.46e-08

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 50.71  E-value: 3.46e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 327 DLTPKKEAVVKILCEEKEvnQEITISLEPTVLDFMKILDPKVGVKFTRMKLFLLREDGR---TDDFSSSDYNmpLHYFKI 403
Cdd:cd17045   1 DLTPPKSAKVTVHFEDQV--ESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEMSifgPEELRFPSRA--LYSYNI 76

                        90
                ....*....|.
gi 25151414 404 EDGDSFLVQEK 414
Cdd:cd17045  77 RDGDEILVEPK 87
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
47-288 7.57e-06

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 48.01  E-value: 7.57e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414  47 ELLVLNNMNIDTIGDSEKLATLashvseaDLGWNQISkwsDIACILKNLPHLRVLNIGHNPLNPvIDHELPVST-LHTII 125
Cdd:COG4886  97 NLTELDLSGNEELSNLTNLESL-------DLSGNQLT---DLPEELANLTNLKELDLSNNQLTD-LPEPLGNLTnLKSLD 165

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 126 LNGTHLpfKTLQSFLSVLPKVTELHMSDNQFNDDddcDEPIS--TTVRTVHLNRCGFlkwSSVMNVVKRFPNVCSVFVCE 203
Cdd:COG4886 166 LSNNQL--TDLPEELGNLTNLKELDLSNNQITDL---PEPLGnlTNLEELDLSGNQL---TDLPEPLANLTNLETLDLSN 237

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414 204 NPLKDVthcKHFEQLPFWNFLNLAKTSIDSWDSLDQLNRMTS-------ISDLRVPNIPLLDALTNEERLHLIIGRLHHL 276
Cdd:COG4886 238 NQLTDL---PELGNLTNLEELDLSNNQLTDLPPLANLTNLKTldlsnnqLTDLKLKELELLLGLNSLLLLLLLLNLLELL 314

                       250
                ....*....|..
gi 25151414 277 RVLNGSKISSEQ 288
Cdd:COG4886 315 ILLLLLTTLLLL 326
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
37-158 9.03e-06

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 47.62  E-value: 9.03e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151414  37 PCKMASQRALELLVLNNMNIDTIGDS----EKLATLashvseaDLGWNQISkwsDIACILKNLPHLRVLNIGHNPLNpvi 112
Cdd:COG4886 129 PEELANLTNLKELDLSNNQLTDLPEPlgnlTNLKSL-------DLSNNQLT---DLPEELGNLTNLKELDLSNNQIT--- 195

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|.
gi 25151414 113 dhELPVS-----TLHTIILNGTHLpfKTLQSFLSVLPKVTELHMSDNQFND 158
Cdd:COG4886 196 --DLPEPlgnltNLEELDLSGNQL--TDLPEPLANLTNLETLDLSNNQLTD 242
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 48-108 6.99e-03

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 37.84  E-value: 6.99e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 25151414  48 LLVLN--NMNIDTIGDsekLATLaSHVSEADLGWNQISKWSDIACILKNLPHLRVLNIGHNPL 108
Cdd:cd21340 122 LRVLNisGNNIDSLEP---LAPL-RNLEQLDASNNQISDLEELLDLLSSWPSLRELDLTGNPV 180
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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