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Conserved domains on  [gi|28572108|ref|NP_788721|]
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salvador, isoform A [Drosophila melanogaster]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SARAH_Sav cd21433
C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar ...
 533-599 2.92e-33

C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar proteins; Sav is a scaffold protein that is mainly found in metazoans. Drosophila melanogaster salvador, also called Shar-pei (SHRP), promotes both cell cycle exit and apoptosis in Drosophila. It plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Human protein salvador homolog 1, also called 45 kDa WW domain protein (WW45), acts as a mammalian sterile 20-like kinase 1 (MST1)-binding protein required to enhance MST1-mediated apoptosis. It is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. This model corresponds to the C-terminal SARAH (Salvador-RassF-Hippo) domain of Sav, which mediates complex formation/heterodimerization between Hippo and Sav.


:

Pssm-ID: 439176  Cd Length: 67  Bit Score: 121.48  E-value: 2.92e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28572108 533 EEIPKWLAVYSEADSSKDHLLQFNMFSLPELEGFDSMLVRLFKQELGTIVGFYERYRRALILEKNRR 599
Cdd:cd21433   1 EEIPEWLQVYARAPSELDHKLKWELFRLPELECFDAMLKRLFKQELEEIVMKYEAYRIALIRELERR 67
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 425-454 1.48e-07

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


:

Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 47.50  E-value: 1.48e-07
                          10        20        30
                  ....*....|....*....|....*....|
gi 28572108   425 LPPGWATQYTLHGRKYYIDHNAHTTHWNHP 454
Cdd:pfam00397   1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
PAT1 super family cl37801
Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate ...
 95-225 9.25e-04

Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate chromosome transmission during cell division.


The actual alignment was detected with superfamily member pfam09770:

Pssm-ID: 401645 [Multi-domain]  Cd Length: 846  Bit Score: 42.33  E-value: 9.25e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572108    95 ARRAQSQMPLQQHSHSH---PHSLPHPSHPHVRSHPPLPPHQFRASSNQLSQNSSNyVNFEQIERMRRQQSSPLLQTTSS 171
Cdd:pfam09770 202 AMRAQAKKPAQQPAPAPaqpPAAPPAQQAQQQQQFPPQIQQQQQPQQQPQQPQQHP-GQGHPVTILQRPQSPQPDPAQPS 280

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 28572108   172 PAPGAGGFQRSYSTTQRQ-----HHPHlggdsydadqgLLSASYANMLQLPQRPHSPAH 225
Cdd:pfam09770 281 IQPQAQQFHQQPPPVPVQptqilQNPN-----------RLSAARVGYPQNPQPGVQPAP 328
 
Name Accession Description Interval E-value
SARAH_Sav cd21433
C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar ...
 533-599 2.92e-33

C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar proteins; Sav is a scaffold protein that is mainly found in metazoans. Drosophila melanogaster salvador, also called Shar-pei (SHRP), promotes both cell cycle exit and apoptosis in Drosophila. It plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Human protein salvador homolog 1, also called 45 kDa WW domain protein (WW45), acts as a mammalian sterile 20-like kinase 1 (MST1)-binding protein required to enhance MST1-mediated apoptosis. It is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. This model corresponds to the C-terminal SARAH (Salvador-RassF-Hippo) domain of Sav, which mediates complex formation/heterodimerization between Hippo and Sav.


Pssm-ID: 439176  Cd Length: 67  Bit Score: 121.48  E-value: 2.92e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28572108 533 EEIPKWLAVYSEADSSKDHLLQFNMFSLPELEGFDSMLVRLFKQELGTIVGFYERYRRALILEKNRR 599
Cdd:cd21433   1 EEIPEWLQVYARAPSELDHKLKWELFRLPELECFDAMLKRLFKQELEEIVMKYEAYRIALIRELERR 67
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 425-454 1.48e-07

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 47.50  E-value: 1.48e-07
                          10        20        30
                  ....*....|....*....|....*....|
gi 28572108   425 LPPGWATQYTLHGRKYYIDHNAHTTHWNHP 454
Cdd:pfam00397   1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
WW cd00201
Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; ...
 426-456 4.11e-07

Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; functions as an interaction module in a diverse set of signalling proteins; binds specific proline-rich sequences but at low affinities compared to other peptide recognition proteins such as antibodies and receptors; WW domains have a single groove formed by a conserved Trp and Tyr which recognizes a pair of residues of the sequence X-Pro; variable loops and neighboring domains confer specificity in this domain; there are five distinct groups based on binding: 1) PPXY motifs 2) the PPLP motif; 3) PGM motifs; 4) PSP or PTP motifs; 5) PR motifs.


Pssm-ID: 238122 [Multi-domain]  Cd Length: 31  Bit Score: 46.37  E-value: 4.11e-07
                        10        20        30
                ....*....|....*....|....*....|.
gi 28572108 426 PPGWATQYTLHGRKYYIDHNAHTTHWNHPLE 456
Cdd:cd00201   1 PPGWEERWDPDGRVYYYNHNTKETQWEDPRE 31
WW smart00456
Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds ...
 424-454 5.75e-06

Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds proline-rich polypeptides.


Pssm-ID: 197736 [Multi-domain]  Cd Length: 33  Bit Score: 43.36  E-value: 5.75e-06
                           10        20        30
                   ....*....|....*....|....*....|.
gi 28572108    424 PLPPGWATQYTLHGRKYYIDHNAHTTHWNHP 454
Cdd:smart00456   1 PLPPGWEERKDPDGRPYYYNHETKETQWEKP 31
PAT1 pfam09770
Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate ...
 95-225 9.25e-04

Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate chromosome transmission during cell division.


Pssm-ID: 401645 [Multi-domain]  Cd Length: 846  Bit Score: 42.33  E-value: 9.25e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572108    95 ARRAQSQMPLQQHSHSH---PHSLPHPSHPHVRSHPPLPPHQFRASSNQLSQNSSNyVNFEQIERMRRQQSSPLLQTTSS 171
Cdd:pfam09770 202 AMRAQAKKPAQQPAPAPaqpPAAPPAQQAQQQQQFPPQIQQQQQPQQQPQQPQQHP-GQGHPVTILQRPQSPQPDPAQPS 280

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 28572108   172 PAPGAGGFQRSYSTTQRQ-----HHPHlggdsydadqgLLSASYANMLQLPQRPHSPAH 225
Cdd:pfam09770 281 IQPQAQQFHQQPPPVPVQptqilQNPN-----------RLSAARVGYPQNPQPGVQPAP 328
 
Name Accession Description Interval E-value
SARAH_Sav cd21433
C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar ...
 533-599 2.92e-33

C-terminal SARAH domain of Drosophila melanogaster scaffold protein salvador (Sav) and similar proteins; Sav is a scaffold protein that is mainly found in metazoans. Drosophila melanogaster salvador, also called Shar-pei (SHRP), promotes both cell cycle exit and apoptosis in Drosophila. It plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Human protein salvador homolog 1, also called 45 kDa WW domain protein (WW45), acts as a mammalian sterile 20-like kinase 1 (MST1)-binding protein required to enhance MST1-mediated apoptosis. It is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. This model corresponds to the C-terminal SARAH (Salvador-RassF-Hippo) domain of Sav, which mediates complex formation/heterodimerization between Hippo and Sav.


Pssm-ID: 439176  Cd Length: 67  Bit Score: 121.48  E-value: 2.92e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28572108 533 EEIPKWLAVYSEADSSKDHLLQFNMFSLPELEGFDSMLVRLFKQELGTIVGFYERYRRALILEKNRR 599
Cdd:cd21433   1 EEIPEWLQVYARAPSELDHKLKWELFRLPELECFDAMLKRLFKQELEEIVMKYEAYRIALIRELERR 67
SARAH_SF cd21883
C-terminal SARAH domain found in scaffold protein salvador (Sav), Ras-association domain ...
 554-598 6.11e-16

C-terminal SARAH domain found in scaffold protein salvador (Sav), Ras-association domain proteins, and mammalian STE20-like protein kinases (MST); The SARAH (Salvador-RassF-Hippo) domain family includes scaffold protein salvador (Sav), Ras-association domain proteins (RASSF1-6), and mammalian STE20-like protein kinase (MST) subfamily members (MST1-2 and Hippo). Sav is a scaffold protein mainly found in metazoans. Drosophila melanogaster Sav, also called Shar-pei (SHRP), promotes both cell cycle exit and apoptosis in Drosophila. It plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Human protein salvador homolog 1, also called 45 kDa WW domain protein (WW45), acts as a mammalian sterile 20-like kinase 1 (MST1)-binding protein required to enhance MST1-mediated apoptosis. It is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway. Classical RASSF proteins interact either directly or indirectly with activated Ras. Ras proteins are small GTPases that are involved in cellular signal transduction. They seem to modulate some of the growth inhibitory responses mediated by Ras and may serve as tumor suppressor genes. RASSF1-6 contains a conserved SARAH motif adjacent to the RA domain that functions in scaffolding and regulatory interactions. MST1/2 and Hippo are involved in a conserved pathway that governs cell contact inhibition, organ size control, and tumor development. MST1 and MST2 are STE20 family stress-activated, pro-apoptotic serine/threonine-protein kinases which, following caspase-cleavage, enter the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Hippo (Hpo), also called STE20-like kinase MST (dMST), is the Drosophila homolog of STE20-like protein kinases, MST1 and MST2. It is a STE20 family serine/threonine-protein kinase that functions as a tumor suppressor by restricting cell proliferation and promotes apoptosis in conjunction with salvador and warts. Hpo also plays a key role in the Hippo/SWH signaling pathway. This model corresponds to the C-terminal SARAH domain, a characteristic coiled-coil structure. It is a small helical module that is important in signal-transduction networks. The central function of the SARAH domain seems to be the mediation of homo- and heterodimerization between SARAH domain-containing proteins.


Pssm-ID: 439177  Cd Length: 45  Bit Score: 71.66  E-value: 6.11e-16
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 28572108 554 QFNMFSLPELEGFDSMLVRLFKQELGTIVGFYERYRRALILEKNR 598
Cdd:cd21883   1 QLKNFSLPELQMFLKMLDPEEEREIEQLVKKYTAYRQAILDALEE 45
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 425-454 1.48e-07

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 47.50  E-value: 1.48e-07
                          10        20        30
                  ....*....|....*....|....*....|
gi 28572108   425 LPPGWATQYTLHGRKYYIDHNAHTTHWNHP 454
Cdd:pfam00397   1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
WW cd00201
Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; ...
 426-456 4.11e-07

Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; functions as an interaction module in a diverse set of signalling proteins; binds specific proline-rich sequences but at low affinities compared to other peptide recognition proteins such as antibodies and receptors; WW domains have a single groove formed by a conserved Trp and Tyr which recognizes a pair of residues of the sequence X-Pro; variable loops and neighboring domains confer specificity in this domain; there are five distinct groups based on binding: 1) PPXY motifs 2) the PPLP motif; 3) PGM motifs; 4) PSP or PTP motifs; 5) PR motifs.


Pssm-ID: 238122 [Multi-domain]  Cd Length: 31  Bit Score: 46.37  E-value: 4.11e-07
                        10        20        30
                ....*....|....*....|....*....|.
gi 28572108 426 PPGWATQYTLHGRKYYIDHNAHTTHWNHPLE 456
Cdd:cd00201   1 PPGWEERWDPDGRVYYYNHNTKETQWEDPRE 31
WW smart00456
Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds ...
 424-454 5.75e-06

Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds proline-rich polypeptides.


Pssm-ID: 197736 [Multi-domain]  Cd Length: 33  Bit Score: 43.36  E-value: 5.75e-06
                           10        20        30
                   ....*....|....*....|....*....|.
gi 28572108    424 PLPPGWATQYTLHGRKYYIDHNAHTTHWNHP 454
Cdd:smart00456   1 PLPPGWEERKDPDGRPYYYNHETKETQWEKP 31
PAT1 pfam09770
Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate ...
 95-225 9.25e-04

Topoisomerase II-associated protein PAT1; Members of this family are necessary for accurate chromosome transmission during cell division.


Pssm-ID: 401645 [Multi-domain]  Cd Length: 846  Bit Score: 42.33  E-value: 9.25e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572108    95 ARRAQSQMPLQQHSHSH---PHSLPHPSHPHVRSHPPLPPHQFRASSNQLSQNSSNyVNFEQIERMRRQQSSPLLQTTSS 171
Cdd:pfam09770 202 AMRAQAKKPAQQPAPAPaqpPAAPPAQQAQQQQQFPPQIQQQQQPQQQPQQPQQHP-GQGHPVTILQRPQSPQPDPAQPS 280

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 28572108   172 PAPGAGGFQRSYSTTQRQ-----HHPHlggdsydadqgLLSASYANMLQLPQRPHSPAH 225
Cdd:pfam09770 281 IQPQAQQFHQQPPPVPVQptqilQNPN-----------RLSAARVGYPQNPQPGVQPAP 328
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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