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Conserved domains on  [gi|31983636|ref|NP_858177|]
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IS150 ORF1(ORF A) (plasmid) [Shigella flexneri 2a str. 301]

Protein Classification

transposase( domain architecture ID 16007956)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

CATH:  1.10.10.10
Gene Ontology:  GO:0046872|GO:0006313|GO:0003677|GO:0004803|GO:0032196|GO:0015074|GO:0006310
PubMed:  11774877|20215432|20885819|17165262|20615441|8831795|15808743

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
transpos_IS3 super family cl41295
IS3 family transposase;
66-173 3.93e-08

IS3 family transposase;


The actual alignment was detected with superfamily member NF033516:

Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 51.41  E-value: 3.93e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   66 ELRIKVVKAVIEQHMSLNQAAAHFMLAGSgSVARWLKVYEERGEAGLRalkigtkrniaisvdpeKAASALELSKDRRIE 145
Cdd:NF033516   1 EFKLEAVREVLEGGKSVAEVARELGISPS-TLYRWRKKYRGGGEAADA-----------------GRLKELLTPEEEENR 62

                         90       100
                 ....*....|....*....|....*...
gi 31983636  146 DLERQVRFLETRLVYLKKLKALAHPTKK 173
Cdd:NF033516  63 RLKRELAELRLENEILKKARKLLRPAVK 90
HTH_28 pfam13518
Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is ...
 10-60 5.49e-07

Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.


:

Pssm-ID: 463908 [Multi-domain]  Cd Length: 52  Bit Score: 44.51  E-value: 5.49e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 31983636    10 KRLEVVNHYfttDDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG 60
Cdd:pfam13518   1 ERLKIVLLA---LEGESIkeAARLFGISRSTVYRWIRRYREGGLEGLLPRRRR 50
 
Name Accession Description Interval E-value
transpos_IS3 NF033516
IS3 family transposase;
66-173 3.93e-08

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 51.41  E-value: 3.93e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   66 ELRIKVVKAVIEQHMSLNQAAAHFMLAGSgSVARWLKVYEERGEAGLRalkigtkrniaisvdpeKAASALELSKDRRIE 145
Cdd:NF033516   1 EFKLEAVREVLEGGKSVAEVARELGISPS-TLYRWRKKYRGGGEAADA-----------------GRLKELLTPEEEENR 62

                         90       100
                 ....*....|....*....|....*...
gi 31983636  146 DLERQVRFLETRLVYLKKLKALAHPTKK 173
Cdd:NF033516  63 RLKRELAELRLENEILKKARKLLRPAVK 90
HTH_28 pfam13518
Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is ...
 10-60 5.49e-07

Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.


Pssm-ID: 463908 [Multi-domain]  Cd Length: 52  Bit Score: 44.51  E-value: 5.49e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 31983636    10 KRLEVVNHYfttDDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG 60
Cdd:pfam13518   1 ERLKIVLLA---LEGESIkeAARLFGISRSTVYRWIRRYREGGLEGLLPRRRR 50
InsE COG2963
Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];
1-68 2.55e-06

Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442203 [Multi-domain]  Cd Length: 93  Bit Score: 43.76  E-value: 2.55e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   1 MSKP--KYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELR 68
Cdd:COG2963   1 MSKKrrRYSPEFKAEAVRLVLEGGASVAEVARELGISPSTLYRWVRQYREGGLGGFPGDGRTTPEQAEIR 70
HTH_28 pfam13518
Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is ...
 68-116 1.32e-04

Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.


Pssm-ID: 463908 [Multi-domain]  Cd Length: 52  Bit Score: 37.96  E-value: 1.32e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 31983636    68 RIKVVKAVIEqHMSLNQAAAHFMLaGSGSVARWLKVYEERGEAGLRALK 116
Cdd:pfam13518   2 RLKIVLLALE-GESIKEAARLFGI-SRSTVYRWIRRYREGGLEGLLPRR 48
transpos_IS630 NF033545
IS630 family transposase;
22-133 1.71e-04

IS630 family transposase;


Pssm-ID: 468076 [Multi-domain]  Cd Length: 298  Bit Score: 40.70  E-value: 1.71e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   22 DDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG---VSADPELRIKVVKAVIEQ------HMSLnQAAAHFM 90
Cdd:NF033545  10 AEGLSIteIAERLGVSRSTVYRWLKRFNEGGLEGLLDKPRPgrpRKLLSEQQAELLALLLEEppegagHWTL-RELAALL 88

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 31983636   91 LAGSG------SVARWLKvyeergEAGLRALKigtKRNIAISVDPEKAA 133
Cdd:NF033545  89 EEEFGveysrsTVRRLLK------RLGLSPKK---PRPRAPKQDPEFVE 128
InsE COG2963
Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];
64-167 7.63e-04

Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442203 [Multi-domain]  Cd Length: 93  Bit Score: 36.83  E-value: 7.63e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636  64 DPELRIKVVKAVIEQHMSLNQAAAHFMLaGSGSVARWLKVYEERGEAGLralkigtkrniaisvdpekAASALELSKDRR 143
Cdd:COG2963   9 SPEFKAEAVRLVLEGGASVAEVARELGI-SPSTLYRWVRQYREGGLGGF-------------------PGDGRTTPEQAE 68

                        90       100
                ....*....|....*....|....
gi 31983636 144 IEDLERQVRFLETRLVYLKKLKAL 167
Cdd:COG2963  69 IRRLRKELRRLEMENDILKKAAAL 92
transpos_ISNCY_2 NF033594
ISNCY family transposase; The ISNCY insertion sequence family, as defined by ISFinder, encodes ...
 70-142 7.48e-03

ISNCY family transposase; The ISNCY insertion sequence family, as defined by ISFinder, encodes several apparently unrelated families of transposases. Members of this family resemble the transposases of ISNCY family elements such as IS1202, ISTde1, ISKpn21, and ISCARN1.


Pssm-ID: 468103 [Multi-domain]  Cd Length: 367  Bit Score: 35.92  E-value: 7.48e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 31983636   70 KVVKAVIEQHMSLNQAAAhfMLAGSG-SVARWLKVYEERGEAGLRALKIGTKRNIAISvdPEKAASALELSKDR 142
Cdd:NF033594   1 KVIQKVVDGRLTVKEAAE--LLGLSErQVRRLLKRYREEGAAGLVHGNRGRPPNNRLP--DELRERVLALYRER 70
 
Name Accession Description Interval E-value
transpos_IS3 NF033516
IS3 family transposase;
66-173 3.93e-08

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 51.41  E-value: 3.93e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   66 ELRIKVVKAVIEQHMSLNQAAAHFMLAGSgSVARWLKVYEERGEAGLRalkigtkrniaisvdpeKAASALELSKDRRIE 145
Cdd:NF033516   1 EFKLEAVREVLEGGKSVAEVARELGISPS-TLYRWRKKYRGGGEAADA-----------------GRLKELLTPEEEENR 62

                         90       100
                 ....*....|....*....|....*...
gi 31983636  146 DLERQVRFLETRLVYLKKLKALAHPTKK 173
Cdd:NF033516  63 RLKRELAELRLENEILKKARKLLRPAVK 90
HTH_28 pfam13518
Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is ...
 10-60 5.49e-07

Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.


Pssm-ID: 463908 [Multi-domain]  Cd Length: 52  Bit Score: 44.51  E-value: 5.49e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 31983636    10 KRLEVVNHYfttDDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG 60
Cdd:pfam13518   1 ERLKIVLLA---LEGESIkeAARLFGISRSTVYRWIRRYREGGLEGLLPRRRR 50
InsE COG2963
Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];
1-68 2.55e-06

Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442203 [Multi-domain]  Cd Length: 93  Bit Score: 43.76  E-value: 2.55e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   1 MSKP--KYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELR 68
Cdd:COG2963   1 MSKKrrRYSPEFKAEAVRLVLEGGASVAEVARELGISPSTLYRWVRQYREGGLGGFPGDGRTTPEQAEIR 70
HTH_28 pfam13518
Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is ...
 68-116 1.32e-04

Helix-turn-helix domain; This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.


Pssm-ID: 463908 [Multi-domain]  Cd Length: 52  Bit Score: 37.96  E-value: 1.32e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 31983636    68 RIKVVKAVIEqHMSLNQAAAHFMLaGSGSVARWLKVYEERGEAGLRALK 116
Cdd:pfam13518   2 RLKIVLLALE-GESIKEAARLFGI-SRSTVYRWIRRYREGGLEGLLPRR 48
Csa3 COG3415
CRISPR-associated protein Csa3, CARF domain [Defense mechanisms]; CRISPR-associated protein ...
 9-79 1.62e-04

CRISPR-associated protein Csa3, CARF domain [Defense mechanisms]; CRISPR-associated protein Csa3, CARF domain is part of the Pathway/BioSystem: CRISPR-Cas system


Pssm-ID: 442641 [Multi-domain]  Cd Length: 325  Bit Score: 40.99  E-value: 1.62e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 31983636   9 EKRLEVVNHYfttDDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSA---DPELRIKVVKAVIEQH 79
Cdd:COG3415  26 ARRLRAVLLL---AEGLSVreIAERLGVSRSTVYRWLKRYREGGLAGLKDRPRGGRPsklSDEQRERLLELLREKS 98
transpos_IS630 NF033545
IS630 family transposase;
22-133 1.71e-04

IS630 family transposase;


Pssm-ID: 468076 [Multi-domain]  Cd Length: 298  Bit Score: 40.70  E-value: 1.71e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636   22 DDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG---VSADPELRIKVVKAVIEQ------HMSLnQAAAHFM 90
Cdd:NF033545  10 AEGLSIteIAERLGVSRSTVYRWLKRFNEGGLEGLLDKPRPgrpRKLLSEQQAELLALLLEEppegagHWTL-RELAALL 88

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 31983636   91 LAGSG------SVARWLKvyeergEAGLRALKigtKRNIAISVDPEKAA 133
Cdd:NF033545  89 EEEFGveysrsTVRRLLK------RLGLSPKK---PRPRAPKQDPEFVE 128
InsE COG2963
Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];
64-167 7.63e-04

Transposase InsE and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442203 [Multi-domain]  Cd Length: 93  Bit Score: 36.83  E-value: 7.63e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983636  64 DPELRIKVVKAVIEQHMSLNQAAAHFMLaGSGSVARWLKVYEERGEAGLralkigtkrniaisvdpekAASALELSKDRR 143
Cdd:COG2963   9 SPEFKAEAVRLVLEGGASVAEVARELGI-SPSTLYRWVRQYREGGLGGF-------------------PGDGRTTPEQAE 68

                        90       100
                ....*....|....*....|....
gi 31983636 144 IEDLERQVRFLETRLVYLKKLKAL 167
Cdd:COG2963  69 IRRLRKELRRLEMENDILKKAAAL 92
HTH_23 pfam13384
Homeodomain-like domain;
9-55 2.27e-03

Homeodomain-like domain;


Pssm-ID: 433164 [Multi-domain]  Cd Length: 50  Bit Score: 34.55  E-value: 2.27e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 31983636     9 EKRLEVVNHYfttDDGYRI--ISARFGVPRTQVRTWVALYEKHGEKGLI 55
Cdd:pfam13384   5 RRRARALLLL---AEGLSVkeIAELLGVSRRTVYRWLKRYNEEGLEGLL 50
transpos_ISNCY_2 NF033594
ISNCY family transposase; The ISNCY insertion sequence family, as defined by ISFinder, encodes ...
 70-142 7.48e-03

ISNCY family transposase; The ISNCY insertion sequence family, as defined by ISFinder, encodes several apparently unrelated families of transposases. Members of this family resemble the transposases of ISNCY family elements such as IS1202, ISTde1, ISKpn21, and ISCARN1.


Pssm-ID: 468103 [Multi-domain]  Cd Length: 367  Bit Score: 35.92  E-value: 7.48e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 31983636   70 KVVKAVIEQHMSLNQAAAhfMLAGSG-SVARWLKVYEERGEAGLRALKIGTKRNIAISvdPEKAASALELSKDR 142
Cdd:NF033594   1 KVIQKVVDGRLTVKEAAE--LLGLSErQVRRLLKRYREEGAAGLVHGNRGRPPNNRLP--DELRERVLALYRER 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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