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DNA ligase B [Klebsiella michiganensis]
Protein Classification
NAD-dependent DNA ligase LigB ( domain architecture ID 11483082 )
NAD-dependent DNA ligase LigB catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction
List of domain hits
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
6-558
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 857.68
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 6 WALV L WMLVGYGQ A A CP A W PQ ARA DR EI ER L S QQ ITE W KN AYW Q QG S S T V S DEVYDQL AE RL AY W R RCF T G EA P AHDAS P 85
Cdd:PRK08097 7 LLIS L LLWSSSAW A V CP D W SP ARA QE EI AA L Q QQ LAQ W DD AYW R QG K S E V D DEVYDQL RA RL TQ W Q RCF G G PE P RDVPL P 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 86 PL K G EAR HPVAHTGV R KLA NQADV ARWM R G Q S G LWVQPKVDGVAVTLVYR Q G R L T QAISRGNGL A GEDWTA RVLQ IP SV P 165
Cdd:PRK08097 87 PL N G KVL HPVAHTGV K KLA DKQAL ARWM A G R S D LWVQPKVDGVAVTLVYR D G K L V QAISRGNGL K GEDWTA KARL IP AI P 166
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 166 KVSD G V LAN S VLQGELFL L REGH V Q K QMGG M NARAKVAG M MMR QQAAAD LNQ L G L F I WAWPDGP QD M KQ RLA L L RQG GF L 245
Cdd:PRK08097 167 QQLP G A LAN L VLQGELFL R REGH I Q Q QMGG I NARAKVAG L MMR KDPSPT LNQ I G V F V WAWPDGP AS M PE RLA Q L ATA GF P 246
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 246 YSALFS HPV AD A QQ V EQ WR Q RW FTS PLPF AS DGVVVR RE K AS PGR F W V PGQG D W VI AWKYPP ASR V M EVR RIS F S VGR S G 325
Cdd:PRK08097 247 LTQRYT HPV KN A EE V AR WR E RW YRA PLPF VT DGVVVR QA K EP PGR Y W Q PGQG E W AV AWKYPP VQQ V A EVR AVQ F A VGR T G 326
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 326 KI A VV AH LEP Q MLDDKRV Q RV SV GSV S RW YNL DI GI GDQ LQI SLAGQGIPR I D S VVWR T A Q R N KP Q PP - A A RF NA L T C YF 404
Cdd:PRK08097 327 KI T VV LE LEP V MLDDKRV S RV NI GSV R RW QQW DI AP GDQ VLV SLAGQGIPR L D K VVWR G A E R T KP T PP d A D RF HS L S C FR 406
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 405 A T P E C A EQFL S RLVWLS SRSV L DI DG V GE AL WR S LH DARSM EHLFSWLA F TPE R L QAI PG VSTL R GQR LWHQFNLAR ER P 484
Cdd:PRK08097 407 A S P G C Q EQFL A RLVWLS GKQG L GL DG I GE GT WR A LH QTGLF EHLFSWLA L TPE Q L ANT PG IGKA R AEQ LWHQFNLAR QQ P 486
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1190266127 485 F L RW IQ A M G V P I P KT A FAR L KEDD WQ RMQE R N E E QW RH LPGIG AE RARQL VT FL H HP D V A ALA K WL SG Q RVP GF 558
Cdd:PRK08097 487 F S RW LK A L G I P L P QA A LNA L DDRS WQ QLLS R S E Q QW QQ LPGIG EG RARQL IA FL Q HP E V K ALA D WL AA Q GIT GF 560
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
6-558
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 857.68
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 6 WALV L WMLVGYGQ A A CP A W PQ ARA DR EI ER L S QQ ITE W KN AYW Q QG S S T V S DEVYDQL AE RL AY W R RCF T G EA P AHDAS P 85
Cdd:PRK08097 7 LLIS L LLWSSSAW A V CP D W SP ARA QE EI AA L Q QQ LAQ W DD AYW R QG K S E V D DEVYDQL RA RL TQ W Q RCF G G PE P RDVPL P 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 86 PL K G EAR HPVAHTGV R KLA NQADV ARWM R G Q S G LWVQPKVDGVAVTLVYR Q G R L T QAISRGNGL A GEDWTA RVLQ IP SV P 165
Cdd:PRK08097 87 PL N G KVL HPVAHTGV K KLA DKQAL ARWM A G R S D LWVQPKVDGVAVTLVYR D G K L V QAISRGNGL K GEDWTA KARL IP AI P 166
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 166 KVSD G V LAN S VLQGELFL L REGH V Q K QMGG M NARAKVAG M MMR QQAAAD LNQ L G L F I WAWPDGP QD M KQ RLA L L RQG GF L 245
Cdd:PRK08097 167 QQLP G A LAN L VLQGELFL R REGH I Q Q QMGG I NARAKVAG L MMR KDPSPT LNQ I G V F V WAWPDGP AS M PE RLA Q L ATA GF P 246
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 246 YSALFS HPV AD A QQ V EQ WR Q RW FTS PLPF AS DGVVVR RE K AS PGR F W V PGQG D W VI AWKYPP ASR V M EVR RIS F S VGR S G 325
Cdd:PRK08097 247 LTQRYT HPV KN A EE V AR WR E RW YRA PLPF VT DGVVVR QA K EP PGR Y W Q PGQG E W AV AWKYPP VQQ V A EVR AVQ F A VGR T G 326
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 326 KI A VV AH LEP Q MLDDKRV Q RV SV GSV S RW YNL DI GI GDQ LQI SLAGQGIPR I D S VVWR T A Q R N KP Q PP - A A RF NA L T C YF 404
Cdd:PRK08097 327 KI T VV LE LEP V MLDDKRV S RV NI GSV R RW QQW DI AP GDQ VLV SLAGQGIPR L D K VVWR G A E R T KP T PP d A D RF HS L S C FR 406
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 405 A T P E C A EQFL S RLVWLS SRSV L DI DG V GE AL WR S LH DARSM EHLFSWLA F TPE R L QAI PG VSTL R GQR LWHQFNLAR ER P 484
Cdd:PRK08097 407 A S P G C Q EQFL A RLVWLS GKQG L GL DG I GE GT WR A LH QTGLF EHLFSWLA L TPE Q L ANT PG IGKA R AEQ LWHQFNLAR QQ P 486
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1190266127 485 F L RW IQ A M G V P I P KT A FAR L KEDD WQ RMQE R N E E QW RH LPGIG AE RARQL VT FL H HP D V A ALA K WL SG Q RVP GF 558
Cdd:PRK08097 487 F S RW LK A L G I P L P QA A LNA L DDRS WQ QLLS R S E Q QW QQ LPGIG EG RARQL IA FL Q HP E V K ALA D WL AA Q GIT GF 560
LIGANc
smart00532 Ligase N family;
29-425
7.56e-92
Ligase N family;
Pssm-ID: 214709 [Multi-domain]
Cd Length: 441
Bit Score: 288.75
E-value: 7.56e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DR EI ER L SQQITEWKNA Y WQQGSSTV SD EV YD Q L AER L AYWRRCF ---- T GEA P AHD - ASP PL K G -- EA RHPV AHTGVR 101
Cdd:smart00532 1 A QK EI SE L RKLLNQHDYR Y YVLDAPII SD AE YD R L MRE L KELEEKH pelk T PDS P TQR v GGK PL E G fn KV RHPV PMLSLD 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLANQADVARWMR ------- GQSGLW V Q PK V DG VA V T L V Y RQ G R L T QA IS RG N G LA GED W T AR V LQ I P S V P - KV S DG V LA 173
Cdd:smart00532 81 NAFDEDELRAFDE riekalg SPFAYV V E PK I DG LS V S L L Y EN G K L V QA AT RG D G TV GED V T QN V KT I R S I P l RL S GD V PE 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 174 NSVLQ GE L F LLR E G ---- HVQKQMG G M ---- N A R AKV AG MMMRQQAA -- A DLN q L GL F IWAWPD G PQ ----- DMKQR L AL 238
Cdd:smart00532 161 RLEVR GE V F MPK E D flal NEELEEE G E kpfa N P R NAA AG SLRQLDPR it A KRK - L RA F FYGLGT G EE lflpk TQSEA L KW 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 239 L RQG GF LY S ALFSH p VAD A QQ V EQWRQR W FT -- SP LP FAS DGVVV R - REK A SPGRF - WVPGQGD W V IA W K Y P PASRVMEV 314
Cdd:smart00532 240 L KEL GF PV S PHTRL - CKN A DE V IEYYEE W EE kr AE LP YEI DGVVV K v DDL A LQREL g FTSKAPR W A IA Y K F P AEEAETKL 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 315 RR I SFS VGR S GKI AV VA H LEP QM L DDKR V Q R VSVGSVSRWYNL DI G IGD QLQISL AG QG IP RIDS VV WR ----- TAQRNK 389
Cdd:smart00532 319 LD I IVQ VGR T GKI TP VA E LEP VF L AGST V S R ATLHNEDEIEEK DI R IGD TVVVRK AG DV IP KVVG VV KE krpgd EREIEM 398
410 420 430 440
....*....|....*....|....*....|....*....|....*
gi 1190266127 390 P QPPAARFNA L T --------- C YF at P E C AE Q FLS R LVWLS SR SV 425
Cdd:smart00532 399 P THCPSCGSE L V reegevdir C PN -- P L C PA Q LIE R IIHFA SR KA 441
LIGANc
cd00114 NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining ...
31-307
8.70e-68
NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor, but using the same basic reaction mechanism. The enzyme reacts with the cofactor to form a phosphoamide-linked AMP with the amino group of a conserved Lysine in the KXDG motif, and subsequently transfers it to the DNA substrate to yield adenylated DNA. This alignment contains members of the NAD+ dependent subfamily only.
Pssm-ID: 238062 [Multi-domain]
Cd Length: 307
Bit Score: 221.70
E-value: 8.70e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 31 RE I ER L SQQITEWKNA Y WQQGSST VSD EV YD Q L AER L AYWRRCFTGEAPAH da SP PLK --------- GEA RHPV AHTGVR 101
Cdd:cd00114 1 ER I AE L RELLNKHDYR Y YVLDEPS VSD AE YD R L YRE L RALEEEHPELKTPD -- SP TQR vggtplsgf KKV RHPV PMLSLD 78
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLANQADVARWMR ------- GQSGLW V Q PK V DG VAVT L V Y RQ G R L T QA IS RG N G LA GED W T AR V LQ I P S V P KVSD G VLAN 174
Cdd:cd00114 79 NAFDEEELRAFDE rikrflg EEPAYV V E PK I DG LSIS L R Y EN G V L V QA AT RG D G TT GED V T EN V RT I R S I P LTLA G APET 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 175 SVLQ GE L F LLREGHVQ -------- KQMGGM N A R AKV AG MMMRQQAA - ADLNQ L GL FI WAWPD ---- GP QDMKQR LA L L RQ 241
Cdd:cd00114 159 LEVR GE V F MPKADFEA lnkereer GEKPFA N P R NAA AG SLRQLDPK i TAKRP L RF FI YGLGE aegl GP KTQSEA LA F L KE 238
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 242 G GF LY S ALFSH p VADAQQ V EQWRQRWFT -- SP LP FAS DGVVV RREKASPG R FWVPGQ -- GD W V IA W K Y P P 307
Cdd:cd00114 239 W GF PV S PETRL - CKNIEE V LAFYDEIEA kr DS LP YEI DGVVV KVDDLALQ R ELGFTS ka PR W A IA Y K F P A 307
dnlj
TIGR00575 DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent ...
36-542
7.07e-51
DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent DNA ligases. Functions of these proteins include DNA repair, DNA replication, and DNA recombination. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). The member of this family from Treponema pallidum differs in having three rather than just one copy of the BRCT (BRCA1 C Terminus) domain (pfam00533) at the C-terminus. It is included in the seed. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273148 [Multi-domain]
Cd Length: 652
Bit Score: 185.18
E-value: 7.07e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 36 L SQQ I TEWKNA Y WQQGSSTV SD EV YD Q L AER L AYWRRCF ---- T GEA P AH - DASP PL KG -- EA RH PVAHTGVRKLANQAD 108
Cdd:TIGR00575 1 L RKL I RHHDYR Y YVLDEPSI SD AE YD R L YRE L QELEEKH peli T PDS P TQ r VGAA PL SR fp KV RH STPMLSLDNAFDEDE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 109 V A RWMR ------- GQSGLW V Q PK V DG VA V T L V Y RQ G R L TQ A IS RG N G LA GED W TA R V LQ I P S V P KVSD G VLANSV L Q -- G 179
Cdd:TIGR00575 81 L A AFIK rirrqlg LKVEYV V E PK I DG LS V S L T Y EN G V L VR A LT RG D G TV GED V TA N V RT I R S I P LRLA G DNPPER L E vr G 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 180 E L F LLR E GHVQKQMGGM -------- N A R AKV AG m MM RQ --- QAA A DLN q L GL F IWAWPD G PQ ---- DMKQR LA L L RQG GF 244
Cdd:TIGR00575 161 E V F MPK E DFEALNEERR eqgekpfa N P R NAA AG - SL RQ ldp RIT A KRK - L RF F AYGLGE G LE lpda TQYEA LA W L KKW GF 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 245 LY S ALFSH p VADAQQ V EQWRQRWFT -- SP LP FAS DGVVV ------- RR E KASPGRF wv P gqg D W V IA W K Y P PASRVMEVR 315
Cdd:TIGR00575 239 PV S PHIRL - CDSIEE V LEYYREIEE kr DS LP YEI DGVVV kvddlal QD E LGFTSKA -- P --- R W A IA Y K F P AEEAQTKLL 312
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 316 RISFS VGR S G K I AV VA H LEP QMLDDKR V Q R VSVGSVSRWYN LDI G IGD QLQISL AG QG IP RIDS V V -- W RT AQ r NK P --- 390
Cdd:TIGR00575 313 DVVVQ VGR T G A I TP VA K LEP VFVAGTT V S R ATLHNEDEIEE LDI R IGD TVVVRK AG DV IP KVVR V L le K RT GS - ER P irf 391
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 391 ------- QP P AARFNALTCYFAT - PE C AE Q FLS R LVWLS SR SVL DI D G V G EALWRS L HDA --- RS MEH L F swl A FTP E R L 459
Cdd:TIGR00575 392 pthcpsc GS P LVKIEEEAVIRCP n LN C PA Q RVE R IKHFA SR NAM DI E G L G DKVIEQ L FEK klv RS VAD L Y --- A LKK E D L 468
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 460 QAIP G VSTLRG Q R L WHQFNLAR E R P FL R WIQ A M G VP - IPKTAFAR L KE -- DDWQRMQERNE E QWRHLP G I G AER A RQL V T 536
Cdd:TIGR00575 469 LELE G FGEKSA Q N L LNAIEKSK E K P LA R LLF A L G IR h VGEVTAKN L AK hf GTLDKLKAASL E ELLSVE G V G PKV A ESI V N 548
....*.
gi 1190266127 537 F L H H P D 542
Cdd:TIGR00575 549 F F H D P N 554
Lig
COG0272 NAD-dependent DNA ligase [Replication, recombination and repair];
29-551
3.86e-47
NAD-dependent DNA ligase [Replication, recombination and repair];
Pssm-ID: 440042 [Multi-domain]
Cd Length: 668
Bit Score: 174.83
E-value: 3.86e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DRE IE R L SQQITEWKNA Y WQQGSSTV SD EV YD Q L AER L ----- AY -- WRR cftgeapah DA SP ------- PL K G -- EA R 92
Cdd:COG0272 6 A KER IE E L REELRRHNYR Y YVLDAPEI SD AE YD A L LRE L qalea EH pe LIT --------- PD SP tqrvgga PL E G fa KV R 76
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 93 H P V A ---------------- HTG VRK LANQAD V A rwmrgqsg LWVQP K V DG V A VT L V Y RQ GRL TQ A IS RG N G LA GED W TA 156
Cdd:COG0272 77 H A V P mlsldnafseeelrdf DRR VRK FLGDEP V E -------- YVCEL K I DG L A IS L R Y EN GRL VR A AT RG D G TV GED V TA 148
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 157 R V LQ I P S V P KVSD G VLANS VL Q -- GE LFLLREGHVQ kqmgg M N ARAKV AG MMM ---- R QQ AA AD L N Q ---------- L GL 220
Cdd:COG0272 149 N V RT I R S I P LRLK G DDVPE VL E vr GE VYMPKADFEA ----- L N EEREE AG EKP fanp R NA AA GS L R Q ldpkitakrp L DF 223
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 221 F IWAW ---- PDG P QDMKQR L A LL RQG GF LYSALFSH p VADAQQ V EQWRQR W FT -- SP LP FAS DGVV V ------- RRE --- 284
Cdd:COG0272 224 F AYGL geve GLL P DTQSEA L E LL KEW GF PVNPERRV - CKSIEE V LAYIEE W EE kr HS LP YEI DGVV I kvndlal QER lgf 302
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 285 -- K A s P g R fwvpgqgd W V IA W K Y P PASRVMEVRR I SFS VGR S G KIAV VA H LEP qmlddkrvqr V S V G -- S VSR -- WY N -- 356
Cdd:COG0272 303 ts R A - P - R -------- W A IA Y K F P AEEATTKLLD I EVQ VGR T G ALTP VA R LEP ---------- V F V A gv T VSR at LH N ed 362
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 357 ---- L D IG IGD QLQISL AG QG IP RIDS VV -- W R TAQRNKPQP P ---------------- AA RF -- N A L T cyfatpe C AE Q 412
Cdd:COG0272 363 eier K D VR IGD TVVVRK AG DV IP EVVG VV le K R PGDEKPFVM P thcpvcgsplvreege AA LR ct N G L S ------- C PA Q 435
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 413 FLS RL VWLS SR SVL DI D G V GE A L WRS L H DA --- RSMEH L FS wla F T P E R L QAIPGVSTLRG Q R L WHQFNLARER P FL R WI 489
Cdd:COG0272 436 LKE RL KHFA SR KAM DI E G L GE K L IEQ L V DA glv KDPAD L YR --- L T K E D L LGLERMGEKSA Q N L LAAIEKSKKT P LA R FL 512
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1190266127 490 Q A M G vp I P ---- K TA FARLKE - DDWQRMQERN EE QWRHLP GIG AER A RQL V T F LHH P D - VAALAKWLS 551
Cdd:COG0272 513 F A L G -- I R hvge T TA KLLARH f GSLDALMAAS EE ELAAVD GIG PVV A ESI V E F FAE P H n RELIERLRA 578
DNA_ligase_aden
pfam01653 NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining ...
29-307
3.12e-23
NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This domain is the catalytic adenylation domain. The NAD+ group is covalently attached to this domain at the lysine in the KXDG motif of this domain. This enzyme- adenylate intermediate is an important feature of the proposed catalytic mechanism.
Pssm-ID: 396292 [Multi-domain]
Cd Length: 318
Bit Score: 100.50
E-value: 3.12e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DREI E R L SQQ I TEWKNA Y WQQGSST V S D EV YD Q L AER L AYWRRCF ---- T GEA P AHD - ASP PL KG -- EA RH PVAHTGVR 101
Cdd:pfam01653 3 A QQQL E E L REL I RKYDYE Y YVLDNPS V P D AE YD R L YRE L KALEEKH peli T PDS P TQR v GAV PL AD fn KV RH LTPMLSLD 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLA N QADVARWMR --------- GQSGLW V Q PK V DG VAVT L V Y RQ G R L TQ A IS RG N G LA GED W TA R V LQ I PSV P KVSD G VL 172
Cdd:pfam01653 83 NAF N LDELQAFIE rirralgnk EKVEYV V E PK I DG LSIS L L Y EN G L L VR A AT RG D G TT GED V TA N V KT I RNI P LKLK G DN 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 173 ANSV L Q -- GE L F LLR E GHVQ kqmgg M N ARAKVA G MMM ---- R QQ AA AD L N Q ---------- L GL F IWAWP ------ D G PQ 230
Cdd:pfam01653 163 PPER L E vr GE V F MPK E DFEA ----- L N EERLEE G EKP fanp R NA AA GS L R Q ldpritakrk L RF F AYGLG lleghe L G FD 237
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 231 DMK Q R LA L L RQG GF LY S A L FSH p VADAQQ V EQWRQR W FT -- SP LP FAS DGVVV ------- R RE KASPGRF wv P gqg D W V I 301
Cdd:pfam01653 238 TQY Q A LA F L KSL GF PV S P L LAL - CDGIEE V LAYYAD W EK kr DS LP YEI DGVVV kvdelal Q RE LGFTAKA -- P --- R W A I 311
....*.
gi 1190266127 302 A W K Y P P 307
Cdd:pfam01653 312 A Y K F P A 317
Name
Accession
Description
Interval
E-value
ligB
PRK08097 NAD-dependent DNA ligase LigB;
6-558
0e+00
NAD-dependent DNA ligase LigB;
Pssm-ID: 236150 [Multi-domain]
Cd Length: 562
Bit Score: 857.68
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 6 WALV L WMLVGYGQ A A CP A W PQ ARA DR EI ER L S QQ ITE W KN AYW Q QG S S T V S DEVYDQL AE RL AY W R RCF T G EA P AHDAS P 85
Cdd:PRK08097 7 LLIS L LLWSSSAW A V CP D W SP ARA QE EI AA L Q QQ LAQ W DD AYW R QG K S E V D DEVYDQL RA RL TQ W Q RCF G G PE P RDVPL P 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 86 PL K G EAR HPVAHTGV R KLA NQADV ARWM R G Q S G LWVQPKVDGVAVTLVYR Q G R L T QAISRGNGL A GEDWTA RVLQ IP SV P 165
Cdd:PRK08097 87 PL N G KVL HPVAHTGV K KLA DKQAL ARWM A G R S D LWVQPKVDGVAVTLVYR D G K L V QAISRGNGL K GEDWTA KARL IP AI P 166
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 166 KVSD G V LAN S VLQGELFL L REGH V Q K QMGG M NARAKVAG M MMR QQAAAD LNQ L G L F I WAWPDGP QD M KQ RLA L L RQG GF L 245
Cdd:PRK08097 167 QQLP G A LAN L VLQGELFL R REGH I Q Q QMGG I NARAKVAG L MMR KDPSPT LNQ I G V F V WAWPDGP AS M PE RLA Q L ATA GF P 246
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 246 YSALFS HPV AD A QQ V EQ WR Q RW FTS PLPF AS DGVVVR RE K AS PGR F W V PGQG D W VI AWKYPP ASR V M EVR RIS F S VGR S G 325
Cdd:PRK08097 247 LTQRYT HPV KN A EE V AR WR E RW YRA PLPF VT DGVVVR QA K EP PGR Y W Q PGQG E W AV AWKYPP VQQ V A EVR AVQ F A VGR T G 326
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 326 KI A VV AH LEP Q MLDDKRV Q RV SV GSV S RW YNL DI GI GDQ LQI SLAGQGIPR I D S VVWR T A Q R N KP Q PP - A A RF NA L T C YF 404
Cdd:PRK08097 327 KI T VV LE LEP V MLDDKRV S RV NI GSV R RW QQW DI AP GDQ VLV SLAGQGIPR L D K VVWR G A E R T KP T PP d A D RF HS L S C FR 406
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 405 A T P E C A EQFL S RLVWLS SRSV L DI DG V GE AL WR S LH DARSM EHLFSWLA F TPE R L QAI PG VSTL R GQR LWHQFNLAR ER P 484
Cdd:PRK08097 407 A S P G C Q EQFL A RLVWLS GKQG L GL DG I GE GT WR A LH QTGLF EHLFSWLA L TPE Q L ANT PG IGKA R AEQ LWHQFNLAR QQ P 486
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1190266127 485 F L RW IQ A M G V P I P KT A FAR L KEDD WQ RMQE R N E E QW RH LPGIG AE RARQL VT FL H HP D V A ALA K WL SG Q RVP GF 558
Cdd:PRK08097 487 F S RW LK A L G I P L P QA A LNA L DDRS WQ QLLS R S E Q QW QQ LPGIG EG RARQL IA FL Q HP E V K ALA D WL AA Q GIT GF 560
LIGANc
smart00532 Ligase N family;
29-425
7.56e-92
Ligase N family;
Pssm-ID: 214709 [Multi-domain]
Cd Length: 441
Bit Score: 288.75
E-value: 7.56e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DR EI ER L SQQITEWKNA Y WQQGSSTV SD EV YD Q L AER L AYWRRCF ---- T GEA P AHD - ASP PL K G -- EA RHPV AHTGVR 101
Cdd:smart00532 1 A QK EI SE L RKLLNQHDYR Y YVLDAPII SD AE YD R L MRE L KELEEKH pelk T PDS P TQR v GGK PL E G fn KV RHPV PMLSLD 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLANQADVARWMR ------- GQSGLW V Q PK V DG VA V T L V Y RQ G R L T QA IS RG N G LA GED W T AR V LQ I P S V P - KV S DG V LA 173
Cdd:smart00532 81 NAFDEDELRAFDE riekalg SPFAYV V E PK I DG LS V S L L Y EN G K L V QA AT RG D G TV GED V T QN V KT I R S I P l RL S GD V PE 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 174 NSVLQ GE L F LLR E G ---- HVQKQMG G M ---- N A R AKV AG MMMRQQAA -- A DLN q L GL F IWAWPD G PQ ----- DMKQR L AL 238
Cdd:smart00532 161 RLEVR GE V F MPK E D flal NEELEEE G E kpfa N P R NAA AG SLRQLDPR it A KRK - L RA F FYGLGT G EE lflpk TQSEA L KW 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 239 L RQG GF LY S ALFSH p VAD A QQ V EQWRQR W FT -- SP LP FAS DGVVV R - REK A SPGRF - WVPGQGD W V IA W K Y P PASRVMEV 314
Cdd:smart00532 240 L KEL GF PV S PHTRL - CKN A DE V IEYYEE W EE kr AE LP YEI DGVVV K v DDL A LQREL g FTSKAPR W A IA Y K F P AEEAETKL 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 315 RR I SFS VGR S GKI AV VA H LEP QM L DDKR V Q R VSVGSVSRWYNL DI G IGD QLQISL AG QG IP RIDS VV WR ----- TAQRNK 389
Cdd:smart00532 319 LD I IVQ VGR T GKI TP VA E LEP VF L AGST V S R ATLHNEDEIEEK DI R IGD TVVVRK AG DV IP KVVG VV KE krpgd EREIEM 398
410 420 430 440
....*....|....*....|....*....|....*....|....*
gi 1190266127 390 P QPPAARFNA L T --------- C YF at P E C AE Q FLS R LVWLS SR SV 425
Cdd:smart00532 399 P THCPSCGSE L V reegevdir C PN -- P L C PA Q LIE R IIHFA SR KA 441
LIGANc
cd00114 NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining ...
31-307
8.70e-68
NAD+ dependent DNA ligase adenylation domain. DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor, but using the same basic reaction mechanism. The enzyme reacts with the cofactor to form a phosphoamide-linked AMP with the amino group of a conserved Lysine in the KXDG motif, and subsequently transfers it to the DNA substrate to yield adenylated DNA. This alignment contains members of the NAD+ dependent subfamily only.
Pssm-ID: 238062 [Multi-domain]
Cd Length: 307
Bit Score: 221.70
E-value: 8.70e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 31 RE I ER L SQQITEWKNA Y WQQGSST VSD EV YD Q L AER L AYWRRCFTGEAPAH da SP PLK --------- GEA RHPV AHTGVR 101
Cdd:cd00114 1 ER I AE L RELLNKHDYR Y YVLDEPS VSD AE YD R L YRE L RALEEEHPELKTPD -- SP TQR vggtplsgf KKV RHPV PMLSLD 78
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLANQADVARWMR ------- GQSGLW V Q PK V DG VAVT L V Y RQ G R L T QA IS RG N G LA GED W T AR V LQ I P S V P KVSD G VLAN 174
Cdd:cd00114 79 NAFDEEELRAFDE rikrflg EEPAYV V E PK I DG LSIS L R Y EN G V L V QA AT RG D G TT GED V T EN V RT I R S I P LTLA G APET 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 175 SVLQ GE L F LLREGHVQ -------- KQMGGM N A R AKV AG MMMRQQAA - ADLNQ L GL FI WAWPD ---- GP QDMKQR LA L L RQ 241
Cdd:cd00114 159 LEVR GE V F MPKADFEA lnkereer GEKPFA N P R NAA AG SLRQLDPK i TAKRP L RF FI YGLGE aegl GP KTQSEA LA F L KE 238
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 242 G GF LY S ALFSH p VADAQQ V EQWRQRWFT -- SP LP FAS DGVVV RREKASPG R FWVPGQ -- GD W V IA W K Y P P 307
Cdd:cd00114 239 W GF PV S PETRL - CKNIEE V LAFYDEIEA kr DS LP YEI DGVVV KVDDLALQ R ELGFTS ka PR W A IA Y K F P A 307
dnlj
TIGR00575 DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent ...
36-542
7.07e-51
DNA ligase, NAD-dependent; All proteins in this family with known functions are NAD-dependent DNA ligases. Functions of these proteins include DNA repair, DNA replication, and DNA recombination. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). The member of this family from Treponema pallidum differs in having three rather than just one copy of the BRCT (BRCA1 C Terminus) domain (pfam00533) at the C-terminus. It is included in the seed. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273148 [Multi-domain]
Cd Length: 652
Bit Score: 185.18
E-value: 7.07e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 36 L SQQ I TEWKNA Y WQQGSSTV SD EV YD Q L AER L AYWRRCF ---- T GEA P AH - DASP PL KG -- EA RH PVAHTGVRKLANQAD 108
Cdd:TIGR00575 1 L RKL I RHHDYR Y YVLDEPSI SD AE YD R L YRE L QELEEKH peli T PDS P TQ r VGAA PL SR fp KV RH STPMLSLDNAFDEDE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 109 V A RWMR ------- GQSGLW V Q PK V DG VA V T L V Y RQ G R L TQ A IS RG N G LA GED W TA R V LQ I P S V P KVSD G VLANSV L Q -- G 179
Cdd:TIGR00575 81 L A AFIK rirrqlg LKVEYV V E PK I DG LS V S L T Y EN G V L VR A LT RG D G TV GED V TA N V RT I R S I P LRLA G DNPPER L E vr G 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 180 E L F LLR E GHVQKQMGGM -------- N A R AKV AG m MM RQ --- QAA A DLN q L GL F IWAWPD G PQ ---- DMKQR LA L L RQG GF 244
Cdd:TIGR00575 161 E V F MPK E DFEALNEERR eqgekpfa N P R NAA AG - SL RQ ldp RIT A KRK - L RF F AYGLGE G LE lpda TQYEA LA W L KKW GF 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 245 LY S ALFSH p VADAQQ V EQWRQRWFT -- SP LP FAS DGVVV ------- RR E KASPGRF wv P gqg D W V IA W K Y P PASRVMEVR 315
Cdd:TIGR00575 239 PV S PHIRL - CDSIEE V LEYYREIEE kr DS LP YEI DGVVV kvddlal QD E LGFTSKA -- P --- R W A IA Y K F P AEEAQTKLL 312
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 316 RISFS VGR S G K I AV VA H LEP QMLDDKR V Q R VSVGSVSRWYN LDI G IGD QLQISL AG QG IP RIDS V V -- W RT AQ r NK P --- 390
Cdd:TIGR00575 313 DVVVQ VGR T G A I TP VA K LEP VFVAGTT V S R ATLHNEDEIEE LDI R IGD TVVVRK AG DV IP KVVR V L le K RT GS - ER P irf 391
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 391 ------- QP P AARFNALTCYFAT - PE C AE Q FLS R LVWLS SR SVL DI D G V G EALWRS L HDA --- RS MEH L F swl A FTP E R L 459
Cdd:TIGR00575 392 pthcpsc GS P LVKIEEEAVIRCP n LN C PA Q RVE R IKHFA SR NAM DI E G L G DKVIEQ L FEK klv RS VAD L Y --- A LKK E D L 468
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 460 QAIP G VSTLRG Q R L WHQFNLAR E R P FL R WIQ A M G VP - IPKTAFAR L KE -- DDWQRMQERNE E QWRHLP G I G AER A RQL V T 536
Cdd:TIGR00575 469 LELE G FGEKSA Q N L LNAIEKSK E K P LA R LLF A L G IR h VGEVTAKN L AK hf GTLDKLKAASL E ELLSVE G V G PKV A ESI V N 548
....*.
gi 1190266127 537 F L H H P D 542
Cdd:TIGR00575 549 F F H D P N 554
Lig
COG0272 NAD-dependent DNA ligase [Replication, recombination and repair];
29-551
3.86e-47
NAD-dependent DNA ligase [Replication, recombination and repair];
Pssm-ID: 440042 [Multi-domain]
Cd Length: 668
Bit Score: 174.83
E-value: 3.86e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DRE IE R L SQQITEWKNA Y WQQGSSTV SD EV YD Q L AER L ----- AY -- WRR cftgeapah DA SP ------- PL K G -- EA R 92
Cdd:COG0272 6 A KER IE E L REELRRHNYR Y YVLDAPEI SD AE YD A L LRE L qalea EH pe LIT --------- PD SP tqrvgga PL E G fa KV R 76
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 93 H P V A ---------------- HTG VRK LANQAD V A rwmrgqsg LWVQP K V DG V A VT L V Y RQ GRL TQ A IS RG N G LA GED W TA 156
Cdd:COG0272 77 H A V P mlsldnafseeelrdf DRR VRK FLGDEP V E -------- YVCEL K I DG L A IS L R Y EN GRL VR A AT RG D G TV GED V TA 148
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 157 R V LQ I P S V P KVSD G VLANS VL Q -- GE LFLLREGHVQ kqmgg M N ARAKV AG MMM ---- R QQ AA AD L N Q ---------- L GL 220
Cdd:COG0272 149 N V RT I R S I P LRLK G DDVPE VL E vr GE VYMPKADFEA ----- L N EEREE AG EKP fanp R NA AA GS L R Q ldpkitakrp L DF 223
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 221 F IWAW ---- PDG P QDMKQR L A LL RQG GF LYSALFSH p VADAQQ V EQWRQR W FT -- SP LP FAS DGVV V ------- RRE --- 284
Cdd:COG0272 224 F AYGL geve GLL P DTQSEA L E LL KEW GF PVNPERRV - CKSIEE V LAYIEE W EE kr HS LP YEI DGVV I kvndlal QER lgf 302
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 285 -- K A s P g R fwvpgqgd W V IA W K Y P PASRVMEVRR I SFS VGR S G KIAV VA H LEP qmlddkrvqr V S V G -- S VSR -- WY N -- 356
Cdd:COG0272 303 ts R A - P - R -------- W A IA Y K F P AEEATTKLLD I EVQ VGR T G ALTP VA R LEP ---------- V F V A gv T VSR at LH N ed 362
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 357 ---- L D IG IGD QLQISL AG QG IP RIDS VV -- W R TAQRNKPQP P ---------------- AA RF -- N A L T cyfatpe C AE Q 412
Cdd:COG0272 363 eier K D VR IGD TVVVRK AG DV IP EVVG VV le K R PGDEKPFVM P thcpvcgsplvreege AA LR ct N G L S ------- C PA Q 435
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 413 FLS RL VWLS SR SVL DI D G V GE A L WRS L H DA --- RSMEH L FS wla F T P E R L QAIPGVSTLRG Q R L WHQFNLARER P FL R WI 489
Cdd:COG0272 436 LKE RL KHFA SR KAM DI E G L GE K L IEQ L V DA glv KDPAD L YR --- L T K E D L LGLERMGEKSA Q N L LAAIEKSKKT P LA R FL 512
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1190266127 490 Q A M G vp I P ---- K TA FARLKE - DDWQRMQERN EE QWRHLP GIG AER A RQL V T F LHH P D - VAALAKWLS 551
Cdd:COG0272 513 F A L G -- I R hvge T TA KLLARH f GSLDALMAAS EE ELAAVD GIG PVV A ESI V E F FAE P H n RELIERLRA 578
ligA
PRK07956 NAD-dependent DNA ligase LigA; Validated
31-551
2.99e-39
NAD-dependent DNA ligase LigA; Validated
Pssm-ID: 236137 [Multi-domain]
Cd Length: 665
Bit Score: 152.20
E-value: 2.99e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 31 RE IE R L SQQITEWKN AY WQQGSSTV SD EV YD Q L AER L aywrrcftgeapahdaspp LKG EA R HP ---------------- 94
Cdd:PRK07956 7 KR IE E L REELNHHAY AY YVLDAPSI SD AE YD R L YRE L ------------------- VAL EA E HP elitpdsptqrvggap 67
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 95 ------ V A H T g V RK L A ----- NQADVARWM - R GQSG L WVQ ------- P K V DG V AV T L V Y RQ G R L TQ A IS RG N G LA GED W T 155
Cdd:PRK07956 68 ldgfek V R H L - V PM L S ldnaf SEEELRAFD k R VRKR L PDP pltylce L K I DG L AV S L L Y EN G V L VR A AT RG D G TT GED I T 146
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 156 A R V LQ I P S V P - KVSDGVLANSVLQ GE L F LLREGHVQ kqmgg M N ARAKVA G MMM ---- R QQ AA AD L N Q ---------- L GL 220
Cdd:PRK07956 147 A N V RT I R S I P l RLHGNEPERLEVR GE V F MPKADFEA ----- L N EERREE G EKP fanp R NA AA GS L R Q ldpkitakrp L SF 221
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 221 F IWAW ----- PDG P QDMKQR L AL L RQG GF LYSALFSH p VADAQQ V EQWRQRWFT -- SP LP FAS DGVV VR ------ R E KA - 286
Cdd:PRK07956 222 F AYGV geveg GEL P DSQSEA L EF L KAW GF PVNPYRKL - CTSIEE V LAFYEEIEE er HD LP YDI DGVV IK vddlal Q E EL g 300
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 287 ---- S P g R fwvpgqgd W V IA W K Y P PASRVMEVRR I SFS VGR S G KIAV VA H LEP qmlddkrvqr V S V G -- S VSR -- WY N -- 356
Cdd:PRK07956 301 ftak A P - R -------- W A IA Y K F P AEEATTKLLD I EVQ VGR T G AVTP VA R LEP ---------- V E V A gv T VSR at LH N ad 361
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 357 ---- L DI G IGD QLQISL AG QG IP RIDS VV wr TAQ R N ------- K P Q -- P ---- PAA R F --------- N A L T cyfatpe C A 410
Cdd:PRK07956 362 eier K DI R IGD TVVVRR AG DV IP EVVG VV -- LEK R P gdereiv M P T hc P vcgs ELV R V egeavlrct N G L S ------- C P 432
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 411 E Q FLS RL VWLS SR SVL DIDG V GE ALWRS L HDA --- RSMEH LF swl AF T P E R L QAIP G VSTLRG Q R L WHQFNLAR E RPFL R 487
Cdd:PRK07956 433 A Q LKE RL IHFV SR NAM DIDG L GE KIIEQ L FEK gli HDPAD LF --- KL T A E D L LGLE G FGEKSA Q N L LDAIEKSK E TSLA R 509
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1190266127 488 WIQ A M G VP - I - P K T A --------- FAR L KEDDWQRMQE rneeqwrh LP G I G AER A RQL V T F LHHPD - VAALAKW L S 551
Cdd:PRK07956 510 FLY A L G IR h V g E K A A kalarhfgs LEA L RAASEEELAA -------- VE G V G EVV A QSI V E F FAVEE n RELIDEL L E 577
ligA
PRK14351 NAD-dependent DNA ligase LigA; Provisional
29-538
2.34e-33
NAD-dependent DNA ligase LigA; Provisional
Pssm-ID: 184640 [Multi-domain]
Cd Length: 689
Bit Score: 134.88
E-value: 2.34e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DREI E R L SQQ I T E WKNA Y WQQGSSTVS D EV YD Q L AE RL AYWRRC F TGE apa HDA SP ------- PL kg EARHP V A H ---- 97
Cdd:PRK14351 32 A EEQA E Q L REA I R E HDHR Y YVEADPVIA D RA YD A L FA RL QALEDA F DLD --- TEN SP trrvgge PL -- DELET V E H vapm 106
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 98 --------- TG VR KLANQ ad V A R WM r G QSGLWVQ PK V DG VA V TL VY RQ G RLTQ A IS RG N G LA G E D W TA R V LQ I P SVP KVS 168
Cdd:PRK14351 107 lsidqsgea DD VR EFDER -- V R R EV - G AVEYVCE PK F DG LS V EV VY ED G EYQR A AT RG D G RE G D D V TA N V RT I R SVP QKL 183
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 169 D G ---- V LA nsv LQ GE LFLLREGHVQKQMGGM -------- N A R AKV AG m MM RQ --- QAA A D l NQ L GL F IWAWP D GPQDMK 233
Cdd:PRK14351 184 R G dypd F LA --- VR GE VYMPKDAFQAYNRERI ergeepfa N P R NAA AG - TL RQ ldp SVV A E - RP L DI F FFDVL D ASELFD 258
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 234 QRLAL L --- RQG G FLYSALFSH p V A D AQQVEQW R Q R WFTS -- P L PFAS DGVV VR - REKASPGRFWVPGQGD - W VI A W K Y P 306
Cdd:PRK14351 259 SHWEE L erf PEW G LRVTDRTER - V D D IDDAIAY R D R LLAA rd D L NYEI DGVV IK v DDRDAREELGATARAP r W AF A Y K F P 337
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 307 PASRVMEV R R I SFS VGR S G KIAV VA H L E P QMLDDKR V Q R V S VGSVSRWYN L DIGI GD QLQISL AG QG IP RIDS VV WRTAQ 386
Cdd:PRK14351 338 ARAEETTI R D I VVQ VGR T G RLTP VA L L D P VDVGGVT V S R A S LHNPAEIEE L GVNV GD RVRVKR AG DV IP YVEE VV EKDSE 417
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 387 RNKPQ P -------- PAA R FNA L T cy F A T P -- E C AE Q FLSRLVWLS SR SV LDI D G V GE ALWRS L H DA RSM E H L FSWLAF T P 456
Cdd:PRK14351 418 GTFEF P dtcpvcds AVE R DGP L A -- F C T G gl A C PA Q LERSIEHYA SR DA LDI E G L GE ERVQQ L V DA GLV E S L ADLYDL T V 495
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 457 ER L QAIP G VSTLRGQR L WHQFNLA RE R P FLRWIQ A M G V P I -- P K TA FARLK E - DDWQRMQERN EE QW R HLPGI G AER A RQ 533
Cdd:PRK14351 496 AD L AELE G WGETSAEN L LAELEAS RE P P LADFLV A L G I P E vg P T TA RNLAR E f GTFEAIMDAD EE AL R AVDDV G PTV A EE 575
....*
gi 1190266127 534 LVT F L 538
Cdd:PRK14351 576 IRE F F 580
DNA_ligase_aden
pfam01653 NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining ...
29-307
3.12e-23
NAD-dependent DNA ligase adenylation domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This domain is the catalytic adenylation domain. The NAD+ group is covalently attached to this domain at the lysine in the KXDG motif of this domain. This enzyme- adenylate intermediate is an important feature of the proposed catalytic mechanism.
Pssm-ID: 396292 [Multi-domain]
Cd Length: 318
Bit Score: 100.50
E-value: 3.12e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 29 A DREI E R L SQQ I TEWKNA Y WQQGSST V S D EV YD Q L AER L AYWRRCF ---- T GEA P AHD - ASP PL KG -- EA RH PVAHTGVR 101
Cdd:pfam01653 3 A QQQL E E L REL I RKYDYE Y YVLDNPS V P D AE YD R L YRE L KALEEKH peli T PDS P TQR v GAV PL AD fn KV RH LTPMLSLD 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 102 KLA N QADVARWMR --------- GQSGLW V Q PK V DG VAVT L V Y RQ G R L TQ A IS RG N G LA GED W TA R V LQ I PSV P KVSD G VL 172
Cdd:pfam01653 83 NAF N LDELQAFIE rirralgnk EKVEYV V E PK I DG LSIS L L Y EN G L L VR A AT RG D G TT GED V TA N V KT I RNI P LKLK G DN 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 173 ANSV L Q -- GE L F LLR E GHVQ kqmgg M N ARAKVA G MMM ---- R QQ AA AD L N Q ---------- L GL F IWAWP ------ D G PQ 230
Cdd:pfam01653 163 PPER L E vr GE V F MPK E DFEA ----- L N EERLEE G EKP fanp R NA AA GS L R Q ldpritakrk L RF F AYGLG lleghe L G FD 237
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 231 DMK Q R LA L L RQG GF LY S A L FSH p VADAQQ V EQWRQR W FT -- SP LP FAS DGVVV ------- R RE KASPGRF wv P gqg D W V I 301
Cdd:pfam01653 238 TQY Q A LA F L KSL GF PV S P L LAL - CDGIEE V LAYYAD W EK kr DS LP YEI DGVVV kvdelal Q RE LGFTAKA -- P --- R W A I 311
....*.
gi 1190266127 302 A W K Y P P 307
Cdd:pfam01653 312 A Y K F P A 317
DNA_ligase_OB
pfam03120 NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the ...
313-388
6.14e-23
NAD-dependent DNA ligase OB-fold domain; DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. This family is a small domain found after the adenylation domain pfam01653 in NAD dependent ligases. OB-fold domains generally are involved in nucleic acid binding.
Pssm-ID: 460813 [Multi-domain]
Cd Length: 79
Bit Score: 92.43
E-value: 6.14e-23
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1190266127 313 EVRR I S F S VGR S G K I AV VA H LEP QM L DDKR V Q R VSVGSVSRWYNL DI G IGD QLQISL AG QG IP RIDS VV WRTAQRN 388
Cdd:pfam03120 3 KLLD I E F Q VGR T G A I TP VA V LEP VE L AGTT V S R ATLHNEDEIKRK DI R IGD TVIVRK AG DV IP EVVG VV LEKRPGD 78
ligA
PRK14350 NAD-dependent DNA ligase LigA; Provisional
32-494
2.80e-21
NAD-dependent DNA ligase LigA; Provisional
Pssm-ID: 172826 [Multi-domain]
Cd Length: 669
Bit Score: 97.98
E-value: 2.80e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 32 EI ER L SQQ I TE W KNA Y WQQG S ST V S D EV YD QLAE RL AY wrrc FTGEA P AHDA -- SP P LK --------- G E AR H PVAHTGV 100
Cdd:PRK14350 8 EI LD L KKL I RK W DKE Y YVDS S PS V E D FT YD KALL RL QE ---- LESKY P EYKT ld SP T LK fgsdllndf K E VE H SFPILSL 83
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 101 R K LANQADVAR W M ---------- RGQS G LW V Q PK V DG VAVT L V Y RQ G R L TQ A IS RG N G LA G E D W T AR V LQ I PS VP k VSDG 170
Cdd:PRK14350 84 D K VYDLKLLKL W I ekmdlensnl GFDF G IS V E PK I DG CSIV L Y Y KD G I L EK A LT RG D G RF G N D V T EN V RT I RN VP - LFID 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 171 VLANS VL Q GE LFLLR E G -- HVQ K QMGG -- M NAR AKVA G MMM R QQAAADL N - Q L GL F IWAWPDGPQDM K QR --- LAL L RQG 242
Cdd:PRK14350 163 EKVEL VL R GE IYITK E N fl KIN K TLEK py T NAR NLAS G ILR R IDSREVA N f P L DI F VYDILYSSLEL K TN hda FDK L KKF 242
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 243 GF LYSAL --------- FSHPVADAQQV E QW R QR wftsp LPFAS DGVV VR ------ RE KAS pgrf WVPGQGD W VI A W K YPP 307
Cdd:PRK14350 243 GF KVNPF crffdgkns IEEILNYVKDI E KK R NS ----- FEYEI DGVV LK vsdfal RE ILG ---- YTSHHPK W SM A Y K FES 313
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 308 A S RVME V RR I SFS VGRSGKI AV VA HL E PQMLDDKRVQRV S VGSVSRWYNLDIGI GD QLQ IS LA G QG IP RIDS V VWRTAQR 387
Cdd:PRK14350 314 L S GFSK V ND I VVQ VGRSGKI TP VA NI E KVFVAGAFITNA S LHNQDYIDSIGLNV GD VVK IS RR G DV IP AVEL V IEKLSVG 393
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1190266127 388 NKPQ P --- P AARFNAL --- TCY F AT - PE C AEQFLS R LVWLS S RSVLD I D G VGEALWRS L HDARSMEHLFSWLA F TPE RL Q 460
Cdd:PRK14350 394 FFKI P dnc P SCKTALI keg AHL F CV n NH C PSVIVE R IKYFC S KKCMN I V G LSDKTIEF L FEKKFISSEIDLYT F NFD RL I 473
490 500 510
....*....|....*....|....*....|....
gi 1190266127 461 AIP G VSTL R GQR L WHQFNLARE RPF LRWIQA MG V 494
Cdd:PRK14350 474 NLK G FKDK R INN L KRSIEASKK RPF SKLLLS MG I 507
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
