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Conserved domains on  [gi|1287693462|gb|PJW53095|]
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SAM-dependent methyltransferase [Escherichia coli]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 18235482)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to Mycobacterium tuberculosis phthiotriol/phenolphthiotriol dimycocerosates methyltransferase

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0032259|GO:0008757|GO:0008168|GO:1904047
PubMed:  12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 77-125 1.67e-14

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


:

Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 66.92  E-value: 1.67e-14
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI 125
Cdd:pfam08241  46 VVGDAEDLPFPDNSFDLVLSSEVLHHVEDPERALREIARVLKPGGILII 94
COG4627 super family cl44094
Predicted SAM-depedendent methyltransferase [General function prediction only];
84-179 4.98e-03

Predicted SAM-depedendent methyltransferase [General function prediction only];


The actual alignment was detected with superfamily member COG4627:

Pssm-ID: 443666 [Multi-domain]  Cd Length: 161  Bit Score: 36.77  E-value: 4.98e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1287693462  84 LPFADKSVDVCLLAHTLPWCTDPH--RLLREADRVLIDDGWLVIS---GFNPISL----MGLRKLVPVLRKTSPYNSRMF 154
Cdd:COG4627    40 LPFPDNSVDAIYSSHVLEHLDYEEapLALKECYRVLKPGGILRIVvpdLEHVARLylaeYDAALDVAELRLAGPIDPLGI 119

                          90       100
                  ....*....|....*....|....*
gi 1287693462 155 tLMRQLDWLSLLNFEVLHASRFHVL 179
Cdd:COG4627   120 -ILGERLAGLAARHSVLFRTGFTRL 143
 
Name Accession Description Interval E-value
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 77-125 1.67e-14

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 66.92  E-value: 1.67e-14
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI 125
Cdd:pfam08241  46 VVGDAEDLPFPDNSFDLVLSSEVLHHVEDPERALREIARVLKPGGILII 94
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 67-140 6.52e-14

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 66.56  E-value: 6.52e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1287693462  67 NVSAQGMPVQ-VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLV 140
Cdd:COG2226    63 RAAEAGLNVEfVVGDAEDLPFPDGSFDLVISSFVLHHLPDPERALAEIARVLKPGGRLVVVDFSPPDLAELEELL 137
MenG_MenH_UbiE TIGR01934
ubiquinone/menaquinone biosynthesis methyltransferases; This model represents a family of ...
 77-143 4.52e-06

ubiquinone/menaquinone biosynthesis methyltransferases; This model represents a family of methyltransferases involved in the biosynthesis of menaquinone and ubiqinone. Some members such as the UbiE enzyme from E. coli are believed to act in both pathways, while others may act in only the menaquinone pathway. These methyltransferases are members of the UbiE/CoQ family of methyltransferases (pfam01209) which also contains ubiquinone methyltransferases and other methyltransferases. Members of this clade include a wide distribution of bacteria and eukaryotes, but no archaea. An outgroup for this clade is provided by the phosphatidylethanolamine methyltransferase (EC 2.1.1.17) from Rhodobacter sphaeroides. Note that a number of non-orthologous genes which are members of pfam03737 have been erroneously annotated as MenG methyltransferases. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]


Pssm-ID: 273884 [Multi-domain]  Cd Length: 223  Bit Score: 46.10  E-value: 4.52e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI--------SGFNPISLMGLRKLVPVL 143
Cdd:TIGR01934  93 IQADAEALPFEDNSFDAVTIAFGLRNVTDIQKALREMYRVLKPGGRLVIlefskpanALLKKFYKFYLKNVLPSI 167
PRK08317 PRK08317
hypothetical protein; Provisional
 70-126 4.58e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 46.08  E-value: 4.58e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1287693462  70 AQGMPVQ-VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVIS 126
Cdd:PRK08317   66 GLGPNVEfVRGDADGLPFPDGSFDAVRSDRVLQHLEDPARALAEIARVLRPGGRVVVL 123
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 54-126 6.48e-04

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 38.18  E-value: 6.48e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1287693462  54 EINCEACAVSHQVNVSAQGMPVQV-QADPLHLPF-ADKSVDVCLLAHTLPW-CTDPHRLLREADRVLIDDGWLVIS 126
Cdd:cd02440    28 DISPVALELARKAAAALLADNVEVlKGDAEELPPeADESFDVIISDPPLHHlVEDLARFLEEARRLLKPGGVLVLT 103
COG4627 COG4627
Predicted SAM-depedendent methyltransferase [General function prediction only];
84-179 4.98e-03

Predicted SAM-depedendent methyltransferase [General function prediction only];


Pssm-ID: 443666 [Multi-domain]  Cd Length: 161  Bit Score: 36.77  E-value: 4.98e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1287693462  84 LPFADKSVDVCLLAHTLPWCTDPH--RLLREADRVLIDDGWLVIS---GFNPISL----MGLRKLVPVLRKTSPYNSRMF 154
Cdd:COG4627    40 LPFPDNSVDAIYSSHVLEHLDYEEapLALKECYRVLKPGGILRIVvpdLEHVARLylaeYDAALDVAELRLAGPIDPLGI 119

                          90       100
                  ....*....|....*....|....*
gi 1287693462 155 tLMRQLDWLSLLNFEVLHASRFHVL 179
Cdd:COG4627   120 -ILGERLAGLAARHSVLFRTGFTRL 143
 
Name Accession Description Interval E-value
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 77-125 1.67e-14

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 66.92  E-value: 1.67e-14
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI 125
Cdd:pfam08241  46 VVGDAEDLPFPDNSFDLVLSSEVLHHVEDPERALREIARVLKPGGILII 94
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 67-140 6.52e-14

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 66.56  E-value: 6.52e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1287693462  67 NVSAQGMPVQ-VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLV 140
Cdd:COG2226    63 RAAEAGLNVEfVVGDAEDLPFPDGSFDLVISSFVLHHLPDPERALAEIARVLKPGGRLVVVDFSPPDLAELEELL 137
UbiG COG2227
2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and ...
 77-129 1.82e-07

2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and metabolism]; 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 441829 [Multi-domain]  Cd Length: 126  Bit Score: 48.48  E-value: 1.82e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVISGFN 129
Cdd:COG2227    74 VQGDLEDLPLEDGSFDLVICSEVLEHLPDPAALLRELARLLKPGGLLLLSTPN 126
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 67-117 1.31e-06

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 45.63  E-value: 1.31e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1287693462  67 NVSAQGMPVQ-VQADPLHLPFADKSVDVCLLAHTLPWCTDP--HRLLREADRVL 117
Cdd:pfam13649  39 RAAEAGLNVEfVQGDAEDLPFPDGSFDLVVSSGVLHHLPDPdlEAALREIARVL 92
MenG_MenH_UbiE TIGR01934
ubiquinone/menaquinone biosynthesis methyltransferases; This model represents a family of ...
 77-143 4.52e-06

ubiquinone/menaquinone biosynthesis methyltransferases; This model represents a family of methyltransferases involved in the biosynthesis of menaquinone and ubiqinone. Some members such as the UbiE enzyme from E. coli are believed to act in both pathways, while others may act in only the menaquinone pathway. These methyltransferases are members of the UbiE/CoQ family of methyltransferases (pfam01209) which also contains ubiquinone methyltransferases and other methyltransferases. Members of this clade include a wide distribution of bacteria and eukaryotes, but no archaea. An outgroup for this clade is provided by the phosphatidylethanolamine methyltransferase (EC 2.1.1.17) from Rhodobacter sphaeroides. Note that a number of non-orthologous genes which are members of pfam03737 have been erroneously annotated as MenG methyltransferases. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]


Pssm-ID: 273884 [Multi-domain]  Cd Length: 223  Bit Score: 46.10  E-value: 4.52e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI--------SGFNPISLMGLRKLVPVL 143
Cdd:TIGR01934  93 IQADAEALPFEDNSFDAVTIAFGLRNVTDIQKALREMYRVLKPGGRLVIlefskpanALLKKFYKFYLKNVLPSI 167
PRK08317 PRK08317
hypothetical protein; Provisional
 70-126 4.58e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 46.08  E-value: 4.58e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1287693462  70 AQGMPVQ-VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVIS 126
Cdd:PRK08317   66 GLGPNVEfVRGDADGLPFPDGSFDAVRSDRVLQHLEDPARALAEIARVLRPGGRVVVL 123
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 54-126 6.48e-04

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 38.18  E-value: 6.48e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1287693462  54 EINCEACAVSHQVNVSAQGMPVQV-QADPLHLPF-ADKSVDVCLLAHTLPW-CTDPHRLLREADRVLIDDGWLVIS 126
Cdd:cd02440    28 DISPVALELARKAAAALLADNVEVlKGDAEELPPeADESFDVIISDPPLHHlVEDLARFLEEARRLLKPGGVLVLT 103
ubiE PRK00216
bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol ...
 77-143 1.04e-03

bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE;


Pssm-ID: 234689 [Multi-domain]  Cd Length: 239  Bit Score: 39.37  E-value: 1.04e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVI--------SGFNPISLMGLRKLVPVL 143
Cdd:PRK00216  108 VQGDAEALPFPDNSFDAVTIAFGLRNVPDIDKALREMYRVLKPGGRLVIlefskptnPPLKKAYDFYLFKVLPLI 182
COG4976 COG4976
Predicted methyltransferase, contains TPR repeat [General function prediction only];
77-126 2.06e-03

Predicted methyltransferase, contains TPR repeat [General function prediction only];


Pssm-ID: 444001 [Multi-domain]  Cd Length: 181  Bit Score: 38.06  E-value: 2.06e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1287693462  77 VQADPLHLPFADKSVDVCLLAHTLPWCTDPHRLLREADRVLIDDGWLVIS 126
Cdd:COG4976    94 LVADLADLAEPDGRFDLIVAADVLTYLGDLAAVFAGVARALKPGGLFIFS 143
COG4627 COG4627
Predicted SAM-depedendent methyltransferase [General function prediction only];
84-179 4.98e-03

Predicted SAM-depedendent methyltransferase [General function prediction only];


Pssm-ID: 443666 [Multi-domain]  Cd Length: 161  Bit Score: 36.77  E-value: 4.98e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1287693462  84 LPFADKSVDVCLLAHTLPWCTDPH--RLLREADRVLIDDGWLVIS---GFNPISL----MGLRKLVPVLRKTSPYNSRMF 154
Cdd:COG4627    40 LPFPDNSVDAIYSSHVLEHLDYEEapLALKECYRVLKPGGILRIVvpdLEHVARLylaeYDAALDVAELRLAGPIDPLGI 119

                          90       100
                  ....*....|....*....|....*
gi 1287693462 155 tLMRQLDWLSLLNFEVLHASRFHVL 179
Cdd:COG4627   120 -ILGERLAGLAARHSVLFRTGFTRL 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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