Tap, RNA-binding; Members of this family adopt a structure consisting of an alpha+beta sandwich with an antiparallel beta-sheet, arranged in a 2(beta-alpha-beta) motif. They are mainly found in mRNA export factors, and mediate the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 119 WFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINPSAPPH 198
Cdd:pfam09162   1 WFKVTIPYGKKYDKKWLLNSIQSLCSVPFTPVDFHYEKNRAVFFVEDASTASALKKVSRKITDEDGRKISIFVNPSAPPY 80

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 333 NLKSLVLHFLQQYYAIYDsGDRQGLLDAYHDGACCSLSIpfipqnparsslaeyfkdsrnvkklkdptlrfRLLKHTRLN 412
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSREG--------------------------------MKQVTGRDA 47

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1331889829 413 VVAFLNELP--KTQHDVNSfvVDISAQTSTLLCFSVNGVFKEVDgksrDSLRAFTRTFIAVPaSNSGLCIVNDELFVRN 489
Cdd:cd00780    48 IVEKLSSLPfqKTKHKITT--VDSQPTPSGGVIVMVTGSLKLDE----QPPRKFSQTFVLAP-QNGGYFVLNDIFRFVD 119

Tap, RNA-binding; Members of this family adopt a structure consisting of an alpha+beta sandwich with an antiparallel beta-sheet, arranged in a 2(beta-alpha-beta) motif. They are mainly found in mRNA export factors, and mediate the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 119 WFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINPSAPPH 198
Cdd:pfam09162   1 WFKVTIPYGKKYDKKWLLNSIQSLCSVPFTPVDFHYEKNRAVFFVEDASTASALKKVSRKITDEDGRKISIFVNPSAPPY 80

UBA-like domain found in nuclear RNA export factor NXF1, NXF2 and similar proteins; The NXF family of mRNA nuclear export factors including vertebrate NXF1 (also called tip-associated protein or mRNA export factor TAP), NXF2 (also called cancer/testis antigen CT39 or TAP-like protein TAPL-2), Caenorhabditis elegans NXF1 (ceNXF1), Saccharomyces cerevisiae mRNA nuclear export factor Mex67p and similar proteins. NXF proteins can stimulate nuclear export of mRNAs and facilitate the export of unspliced viral mRNA containing the constitutive transport element. It is a multi-domain protein with a nuclear localization sequence (NLS), a non-canonical mRNA-binding domain, and four leucine-rich repeats (LLR) at the N-terminal region. Its C-terminal part contains a NTF2-like domain and a ubiquitin-associated (UBA)-like domain, joined by flexible Pro-rich linker. Caenorhabditis elegans NXF1 are essential for the nuclear export of poly(A)+mRNA. In budding yeast, Mex67p binds mRNAs through its adaptor Yra1/REF. It also interacts directly with Nab2, an essential shuttling mRNA-binding protein required for export. Moreover, Mex67p associates with both nuclear pore protein (nucleoporin) FG repeats and Hpr1, a component of the TREX/THO complex linking transcription and export.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1331889829 518 QQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAF 568
Cdd:cd14342     1 QQQMVQQLSAQTGMNLEWSQKCLEDNGWDYEKALANFEELKAQGKIPPEAF 51

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 333 NLKSLVLHFLQQYYAIYDsGDRQGLLDAYHDGACCSLSIpfipqnparsslaeyfkdsrnvkklkdptlrfRLLKHTRLN 412
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSREG--------------------------------MKQVTGRDA 47

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1331889829 413 VVAFLNELP--KTQHDVNSfvVDISAQTSTLLCFSVNGVFKEVDgksrDSLRAFTRTFIAVPaSNSGLCIVNDELFVRN 489
Cdd:cd00780    48 IVEKLSSLPfqKTKHKITT--VDSQPTPSGGVIVMVTGSLKLDE----QPPRKFSQTFVLAP-QNGGYFVLNDIFRFVD 119

Leucine-rich repeat (LRR) protein [Transcription];

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1331889829 263 NIPELLSLNLSNNRLyrlDDMSSIVQKVPNLKILNLSGNEdGHELPPPIA 312
Cdd:COG4886   180 NLTNLKELDLSNNQI---TDLPEPLGNLTNLEELDLSGNQ-LTDLPEPLA 225

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.

                          10        20        30
                  ....*....|....*....|....*....|....*
gi 1331889829 267 LLSLNLSNNRLYRLDDMSSIVQKVPNLKILNLSGN 301
Cdd:cd21340   144 LEQLDASNNQISDLEELLDLLSSWPSLRELDLTGN 178

Tap, RNA-binding; Members of this family adopt a structure consisting of an alpha+beta sandwich with an antiparallel beta-sheet, arranged in a 2(beta-alpha-beta) motif. They are mainly found in mRNA export factors, and mediate the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 119 WFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINPSAPPH 198
Cdd:pfam09162   1 WFKVTIPYGKKYDKKWLLNSIQSLCSVPFTPVDFHYEKNRAVFFVEDASTASALKKVSRKITDEDGRKISIFVNPSAPPY 80

UBA-like domain found in nuclear RNA export factor NXF1, NXF2 and similar proteins; The NXF family of mRNA nuclear export factors including vertebrate NXF1 (also called tip-associated protein or mRNA export factor TAP), NXF2 (also called cancer/testis antigen CT39 or TAP-like protein TAPL-2), Caenorhabditis elegans NXF1 (ceNXF1), Saccharomyces cerevisiae mRNA nuclear export factor Mex67p and similar proteins. NXF proteins can stimulate nuclear export of mRNAs and facilitate the export of unspliced viral mRNA containing the constitutive transport element. It is a multi-domain protein with a nuclear localization sequence (NLS), a non-canonical mRNA-binding domain, and four leucine-rich repeats (LLR) at the N-terminal region. Its C-terminal part contains a NTF2-like domain and a ubiquitin-associated (UBA)-like domain, joined by flexible Pro-rich linker. Caenorhabditis elegans NXF1 are essential for the nuclear export of poly(A)+mRNA. In budding yeast, Mex67p binds mRNAs through its adaptor Yra1/REF. It also interacts directly with Nab2, an essential shuttling mRNA-binding protein required for export. Moreover, Mex67p associates with both nuclear pore protein (nucleoporin) FG repeats and Hpr1, a component of the TREX/THO complex linking transcription and export.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1331889829 518 QQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAF 568
Cdd:cd14342     1 QQQMVQQLSAQTGMNLEWSQKCLEDNGWDYEKALANFEELKAQGKIPPEAF 51

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 333 NLKSLVLHFLQQYYAIYDsGDRQGLLDAYHDGACCSLSIpfipqnparsslaeyfkdsrnvkklkdptlrfRLLKHTRLN 412
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSREG--------------------------------MKQVTGRDA 47

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1331889829 413 VVAFLNELP--KTQHDVNSfvVDISAQTSTLLCFSVNGVFKEVDgksrDSLRAFTRTFIAVPaSNSGLCIVNDELFVRN 489
Cdd:cd00780    48 IVEKLSSLPfqKTKHKITT--VDSQPTPSGGVIVMVTGSLKLDE----QPPRKFSQTFVLAP-QNGGYFVLNDIFRFVD 119

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331889829 341 FLQQYYAIYDSGDRQGLLDAYHDGAccslsipfipqnparsslaeYFKDSRNVKKLKDPTLRFRLLKHtrlnvVAFLNEL 420
Cdd:cd00531     4 FLYRYARLLDAGDREWLALLYADDA--------------------YFEPPGGDGLIYPDDGREAIEDR-----VRRLPFG 58

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1331889829 421 PKTQHDVNSFVVDISAQTSTLLCFSVNGVFkEVDGKSRDSLRAFTRTFIAVPaSNSGLCIVNDELF 486
Cdd:cd00531    59 PSRTRHLVSNVDVQPGDDGEGVVVSVFGVL-RTRGDGEQDVFAGGQTFVLRP-QGGGGKIANRRFR 122

Leucine Rich repeats (2 copies); Leucine rich repeats are short sequence motifs present in a number of proteins with diverse functions and cellular locations. These repeats are usually involved in protein-protein interactions. Each Leucine Rich Repeat is composed of a beta-alpha unit. These units form elongated non-globular structures. Leucine Rich Repeats are often flanked by cysteine rich domains.

                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1331889829 265 PELLSLNLSNNRLYRLDdmssIVQKVPNLKILNLSGN 301
Cdd:pfam12799   1 PNLEVLDLSNNQITDIP----PLAKLPNLETLDLSGN 33

Leucine rich repeat;

                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 1331889829 265 PELLSLNLSNNRLYRLDDmsSIVQKVPNLKILNLSGNE 302
Cdd:pfam13855   1 PNLRSLDLSNNRLTSLDD--GAFKGLSNLKVLDLSNNL 36