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Conserved domains on  [gi|1359351544|gb|PRT20279|]
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aminoglycoside phosphotransferase [Bacillus toyonensis]

Protein Classification

protein kinase family protein( domain architecture ID 229378)

protein kinase family protein may catalyze the transfer of the gamma-phosphoryl group from ATP to substrates such as serine/threonine and/or tyrosine residues on proteins, or may be a pseudokinase

CATH:  1.10.510.10
PubMed:  16244704
SCOP:  4003661

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SrkA super family cl34459
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 37-163 4.66e-11

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


The actual alignment was detected with superfamily member COG2334:

Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 61.86  E-value: 4.66e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  37 HKLLRHLENKGFHyAPK---------FLGIDEKDREILSFIEGDAGNYPlkeymwSNDVLKEIAKMLRLYHDAVSDFPL- 106
Cdd:COG2334    58 LALLAHLAAAGLP-VPApvptrdgetLLELEGRPAALFPFLPGRSPEEP------SPEQLEELGRLLARLHRALADFPRp 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544 107 ----LDDWK--------PMDHTPHNIEVL----------------------CHNDFAIYNIIFNNEKPVGIIDFDVAAPG 152
Cdd:COG2334   131 nardLAWWDellerllgPLLPDPEDRALLeelldrlearlapllgalprgvIHGDLHPDNVLFDGDGVSGLIDFDDAGYG 210

                         170
                  ....*....|.
gi 1359351544 153 PRMWDIAYTLY 163
Cdd:COG2334   211 PRLYDLAIALN 221
 
Name Accession Description Interval E-value
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 37-163 4.66e-11

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 61.86  E-value: 4.66e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  37 HKLLRHLENKGFHyAPK---------FLGIDEKDREILSFIEGDAGNYPlkeymwSNDVLKEIAKMLRLYHDAVSDFPL- 106
Cdd:COG2334    58 LALLAHLAAAGLP-VPApvptrdgetLLELEGRPAALFPFLPGRSPEEP------SPEQLEELGRLLARLHRALADFPRp 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544 107 ----LDDWK--------PMDHTPHNIEVL----------------------CHNDFAIYNIIFNNEKPVGIIDFDVAAPG 152
Cdd:COG2334   131 nardLAWWDellerllgPLLPDPEDRALLeelldrlearlapllgalprgvIHGDLHPDNVLFDGDGVSGLIDFDDAGYG 210

                         170
                  ....*....|.
gi 1359351544 153 PRMWDIAYTLY 163
Cdd:COG2334   211 PRLYDLAIALN 221
ChoK-like cd05151
Choline Kinase and similar proteins; This subfamily is composed of bacterial and eukaryotic ...
 37-159 1.09e-10

Choline Kinase and similar proteins; This subfamily is composed of bacterial and eukaryotic choline kinases, as well as eukaryotic ethanolamine kinase. ChoK catalyzes the transfer of the gamma-phosphoryl group from ATP (or CTP) to its substrate, choline, producing phosphorylcholine (PCho), a precursor to the biosynthesis of two major membrane phospholipids, phosphatidylcholine (PC), and sphingomyelin (SM). Although choline is the preferred substrate, ChoK also shows substantial activity towards ethanolamine and its N-methylated derivatives. Bacterial ChoK is also referred to as licA protein. ETNK catalyzes the transfer of the gamma-phosphoryl group from CTP to ethanolamine (Etn), the first step in the CDP-Etn pathway for the formation of the major phospholipid, phosphatidylethanolamine (PtdEtn). Unlike ChoK, ETNK shows specific activity for its substrate and displays negligible activity towards N-methylated derivatives of Etn. ChoK plays an important role in cell signaling pathways and the regulation of cell growth. The ChoK subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270700 [Multi-domain]  Cd Length: 152  Bit Score: 58.34  E-value: 1.09e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  37 HKLLRHLENKGFhyAPKFLGIDEKDR-EILSFIEGDAgnyPLKEYMWSNDVLKEIAKMLRLYHDavsdFPLLDDwkpmdh 115
Cdd:cd05151    43 KANSKAAAELGI--APEVIYFDPETGvKITEFIEGAT---LLTNDFSDPENLERIAALLRKLHS----SPLEDL------ 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1359351544 116 tphnieVLCHNDFAIYNIIFNNEKPVgIIDFDVAAPGPRMWDIA 159
Cdd:cd05151   108 ------VLCHNDLVPGNFLLDDDRLY-LIDWEYAGMNDPLFDLA 144
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 26-163 5.57e-09

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 55.20  E-value: 5.57e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  26 RRELKPDSTNIHKLLRHLENKGFHYAPKFLGIDE------KDREILSFIEG-DAGNYPLKEymWSNDVLKEIAKMLRLYH 98
Cdd:pfam01636  30 PGRAAEELRRELALLRHLAAAGVPPVPRVLAGCTdaellgLPFLLMEYLPGeVLARPLLPE--ERGALLEALGRALARLH 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  99 DA---------VSDFPLLDDWKPMDHTPHNI----------------------------EVLCHNDFAIYNIIFNNEKPV 141
Cdd:pfam01636 108 AVdpaalplagRLARLLELLRQLEAALARLLaaelldrleeleerllaallallpaelpPVLVHGDLHPGNLLVDPGGRV 187

                         170       180
                  ....*....|....*....|...
gi 1359351544 142 -GIIDFDVAAPGPRMWDIAYTLY 163
Cdd:pfam01636 188 sGVIDFEDAGLGDPAYDLAILLN 210
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 122-166 2.59e-03

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 38.08  E-value: 2.59e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|
gi 1359351544  122 VLCHNDFAIYNIIFNNEKP-----VGIIDFDVAAPGPRMWDIAYTLYTCV 166
Cdd:smart00587 121 VLNHGDLWANNIMFKYDDEgkpedVALIDFQLSHYGSPAEDLHYFLLTSL 170
 
Name Accession Description Interval E-value
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 37-163 4.66e-11

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 61.86  E-value: 4.66e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  37 HKLLRHLENKGFHyAPK---------FLGIDEKDREILSFIEGDAGNYPlkeymwSNDVLKEIAKMLRLYHDAVSDFPL- 106
Cdd:COG2334    58 LALLAHLAAAGLP-VPApvptrdgetLLELEGRPAALFPFLPGRSPEEP------SPEQLEELGRLLARLHRALADFPRp 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544 107 ----LDDWK--------PMDHTPHNIEVL----------------------CHNDFAIYNIIFNNEKPVGIIDFDVAAPG 152
Cdd:COG2334   131 nardLAWWDellerllgPLLPDPEDRALLeelldrlearlapllgalprgvIHGDLHPDNVLFDGDGVSGLIDFDDAGYG 210

                         170
                  ....*....|.
gi 1359351544 153 PRMWDIAYTLY 163
Cdd:COG2334   211 PRLYDLAIALN 221
ChoK-like cd05151
Choline Kinase and similar proteins; This subfamily is composed of bacterial and eukaryotic ...
 37-159 1.09e-10

Choline Kinase and similar proteins; This subfamily is composed of bacterial and eukaryotic choline kinases, as well as eukaryotic ethanolamine kinase. ChoK catalyzes the transfer of the gamma-phosphoryl group from ATP (or CTP) to its substrate, choline, producing phosphorylcholine (PCho), a precursor to the biosynthesis of two major membrane phospholipids, phosphatidylcholine (PC), and sphingomyelin (SM). Although choline is the preferred substrate, ChoK also shows substantial activity towards ethanolamine and its N-methylated derivatives. Bacterial ChoK is also referred to as licA protein. ETNK catalyzes the transfer of the gamma-phosphoryl group from CTP to ethanolamine (Etn), the first step in the CDP-Etn pathway for the formation of the major phospholipid, phosphatidylethanolamine (PtdEtn). Unlike ChoK, ETNK shows specific activity for its substrate and displays negligible activity towards N-methylated derivatives of Etn. ChoK plays an important role in cell signaling pathways and the regulation of cell growth. The ChoK subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270700 [Multi-domain]  Cd Length: 152  Bit Score: 58.34  E-value: 1.09e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  37 HKLLRHLENKGFhyAPKFLGIDEKDR-EILSFIEGDAgnyPLKEYMWSNDVLKEIAKMLRLYHDavsdFPLLDDwkpmdh 115
Cdd:cd05151    43 KANSKAAAELGI--APEVIYFDPETGvKITEFIEGAT---LLTNDFSDPENLERIAALLRKLHS----SPLEDL------ 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1359351544 116 tphnieVLCHNDFAIYNIIFNNEKPVgIIDFDVAAPGPRMWDIA 159
Cdd:cd05151   108 ------VLCHNDLVPGNFLLDDDRLY-LIDWEYAGMNDPLFDLA 144
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 26-163 5.57e-09

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 55.20  E-value: 5.57e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  26 RRELKPDSTNIHKLLRHLENKGFHYAPKFLGIDE------KDREILSFIEG-DAGNYPLKEymWSNDVLKEIAKMLRLYH 98
Cdd:pfam01636  30 PGRAAEELRRELALLRHLAAAGVPPVPRVLAGCTdaellgLPFLLMEYLPGeVLARPLLPE--ERGALLEALGRALARLH 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  99 DA---------VSDFPLLDDWKPMDHTPHNI----------------------------EVLCHNDFAIYNIIFNNEKPV 141
Cdd:pfam01636 108 AVdpaalplagRLARLLELLRQLEAALARLLaaelldrleeleerllaallallpaelpPVLVHGDLHPGNLLVDPGGRV 187

                         170       180
                  ....*....|....*....|...
gi 1359351544 142 -GIIDFDVAAPGPRMWDIAYTLY 163
Cdd:pfam01636 188 sGVIDFEDAGLGDPAYDLAILLN 210
Bud32 COG3642
tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and ...
 38-196 7.58e-09

tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and biogenesis]; tRNA A-37 threonylcarbamoyl transferase component Bud32 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 442859 [Multi-domain]  Cd Length: 159  Bit Score: 53.42  E-value: 7.58e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  38 KLLRHLENKGFHyAPKFLGIDEKDREIL-SFIEGDagnyPLKEYMWSN----DVLKEIAKMLRLYHDAvsdfpllddwkp 112
Cdd:COG3642     8 RLLRELREAGVP-VPKVLDVDPDDADLVmEYIEGE----TLADLLEEGelppELLRELGRLLARLHRA------------ 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544 113 mdhtphNIevlCHNDFAIYNIIFNNEKPVgIIDFDVAAPGPRM----WDIAYTLYTcvpLSRVYYTESGEAV-----HYD 183
Cdd:COG3642    71 ------GI---VHGDLTTSNILVDDGGVY-LIDFGLARYSDPLedkaVDLAVLKRS---LESTHPDPAEELWeafleGYR 137

                         170
                  ....*....|...
gi 1359351544 184 SSQHAGRIKERVK 196
Cdd:COG3642   138 EVGPAEEVLRRLR 150
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 40-163 1.30e-08

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 52.69  E-value: 1.30e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  40 LRHLENKGFHYAPKFLGIDEKDRE---ILSFIEGD--AGNYPLKEYMWSNDVLKEIAKMLRLYHDavsdfpllddwkpmd 114
Cdd:cd05120    43 LQLLAGKLSLPVPKVYGFGESDGWeylLMERIEGEtlSEVWPRLSEEEKEKIADQLAEILAALHR--------------- 107

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1359351544 115 htpHNIEVLCHNDFAIYNIIFNNE-KPVGIIDFDVAAPGPRMWDIAYTLY 163
Cdd:cd05120   108 ---IDSSVLTHGDLHPGNILVKPDgKLSGIIDWEFAGYGPPAFDYAAALR 154
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
122-165 1.34e-07

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 49.78  E-value: 1.34e-07
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1359351544 122 VLCHNDFAIYNIIFNNEKPVGIIDFDVAAPGPRMWDIAYTLYTC 165
Cdd:COG0510    50 VLCHGDLHPGNFLVTDDGRLYLIDWEYAGLGDPAFDLAALLVEY 93
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 107-162 2.46e-07

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 50.50  E-value: 2.46e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1359351544 107 LDDWKPMDHTPhnieVLCHNDFAIYNIIFNNEKP--VGIIDFDVAAPGPRMWDIAYTL 162
Cdd:COG3173   182 LAANLPEWGPP----VLVHGDLRPGNLLVDPDDGrlTAVIDWELATLGDPAADLAYLL 235
Choline_kinase pfam01633
Choline/ethanolamine kinase; Choline kinase catalyzes the committed step in the synthesis of ...
 49-159 1.48e-06

Choline/ethanolamine kinase; Choline kinase catalyzes the committed step in the synthesis of phosphatidylcholine by the CDP-choline pathway. This alignment covers the protein kinase portion of the protein. The divergence of this family makes it very difficult to create a model that specifically predicts choline/ethanolamine kinases only. However if [add Pfam ID here for Choline_kinase_C] is also present then it is definitely a member of this family.


Pssm-ID: 396278 [Multi-domain]  Cd Length: 211  Bit Score: 47.65  E-value: 1.48e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  49 HYAPKFLGIDEKDReILSFIEGdagnYPLK-EYMWSNDVLKEIAKMLRLYHDAV-------------------------- 101
Cdd:pfam01633  34 GLGPKLYGFFPNGR-IEEFIPS----RTLStEDLRDPEISKLIAKRLAELHSLEmpgkkspslwktmrkwlsllknlgap 108

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1359351544 102 -----SDFPLLDDWKPMDHTPHNIE----------VLCHNDFAIYNIIFNNE-KPVGIIDFDVAAPGPRMWDIA 159
Cdd:pfam01633 109 esvnkSEQLKSINLEDLEKEINKLEkwlelldspiVFCHNDLQSGNILLLNEtKRLVLIDFEYASYNYRGFDIA 182
HomoserineK_II cd05153
Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a ...
 107-162 3.31e-06

Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a subset of bacteria, which have a Protein Kinase fold. These proteins do not bear any similarity to the GHMP family homoserine kinases present in most bacteria and eukaryotes. Homoserine kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to L-homoserine producing L-homoserine phosphate, an intermediate in the production of the amino acids threonine, methionine, and isoleucine. The Type II homoserine kinase subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270702 [Multi-domain]  Cd Length: 300  Bit Score: 47.25  E-value: 3.31e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1359351544 107 LDDWKPMDHT--PHnieVLCHNDFAIYNIIFNNEKPVGIIDFDVAAPGPRMWDIAYTL 162
Cdd:cd05153   166 LARLQALAPSdlPR---GVIHADLFRDNVLFDGDRLSGIIDFYDACYDPLLYDLAIAL 220
ChoK-like_euk cd14021
Euykaryotic Choline Kinase and similar proteins; This group is composed of eukaryotic choline ...
 49-159 7.32e-06

Euykaryotic Choline Kinase and similar proteins; This group is composed of eukaryotic choline kinase, ethanolamine kinase, and similar proteins. ChoK catalyzes the transfer of the gamma-phosphoryl group from ATP (or CTP) to its substrate, choline, producing phosphorylcholine (PCho), a precursor to the biosynthesis of two major membrane phospholipids, phosphatidylcholine (PC), and sphingomyelin (SM). Although choline is the preferred substrate, ChoK also shows substantial activity towards ethanolamine and its N-methylated derivatives. ETNK catalyzes the transfer of the gamma-phosphoryl group from CTP to ethanolamine (Etn), the first step in the CDP-Etn pathway for the formation of the major phospholipid, phosphatidylethanolamine (PtdEtn). Unlike ChoK, ETNK shows specific activity for its substrate and displays negligible activity towards N-methylated derivatives of Etn. ChoK plays an important role in cell signaling pathways and the regulation of cell growth. The ChoK subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270923 [Multi-domain]  Cd Length: 229  Bit Score: 45.72  E-value: 7.32e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  49 HYAPKFLGIDEKDReILSFIEGDagnyPLK-EYMWSNDVLKEIAKMLRLYHDavsdfpllddwkpmDHTPhNIeVLCHND 127
Cdd:cd14021    60 GLGPKLIYKFDGGR-IEEYIDGR----PLTtDELRNPSVLTSIAKLLAKFHK--------------IKTP-PV-VFCHND 118

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1359351544 128 FAIYNII-FNNEKPVGIIDFDVAAPGPRMWDIA 159
Cdd:cd14021   119 LQENNILlTNDQDGLRLIDFEYSGFNYRGYDIA 151
ACAD10_11_N-like cd05154
N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This ...
 89-165 5.92e-05

N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This subfamily is composed of the N-terminal domains of vertebrate ACAD10 and ACAD11, and similar uncharacterized bacterial and eukaryotic proteins. ACADs are a family of flavoproteins that are involved in the beta-oxidation of fatty acyl-CoA derivatives. ACAD deficiency can cause metabolic disorders including muscle fatigue, hypoglycemia, and hepatic lipidosis. There are at least 11 distinct ACADs, some of which show distinct substrate specificities to either straight-chain or branched-chain fatty acids. ACAD10 is widely expressed in human tissues and highly expressed in liver, kidney, pancreas, and spleen. ACAD10 and ACAD11 are both significantly expressed in human brain tissues. They contain a long N-terminal domain with similarity to phosphotransferases with a Protein Kinase fold, which is absent in other ACADs. They may exhibit multiple functions in acyl-CoA oxidation pathways. ACAD11 utilizes substrates with carbon chain lengths of 20 to 26, with optimal activity towards C22CoA. ACAD10 may be associated with an increased risk in type II diabetes. The ACAD10/11-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270703 [Multi-domain]  Cd Length: 254  Bit Score: 43.37  E-value: 5.92e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359351544  89 EIAKMLRLYHDAVSDF-PLLD---DW----KPMDHTPhnieVLCHNDFAIYNIIFNNE-KPVGIIDFDVAAPGPRMWDIA 159
Cdd:cd05154   141 QVDRWRRQLEAAATDPpPALEealRWlranLPADGRP----VLVHGDFRLGNLLFDPDgRVTAVLDWELATLGDPLEDLA 216


                  ....*.
gi 1359351544 160 YTLYTC 165
Cdd:cd05154   217 WLLARW 222
ETNK_euk cd05157
Euykaryotic Ethanolamine kinase; ETNK catalyzes the transfer of the gamma-phosphoryl group ...
 122-159 2.71e-04

Euykaryotic Ethanolamine kinase; ETNK catalyzes the transfer of the gamma-phosphoryl group from CTP to ethanolamine (Etn), the first step in the CDP-Etn pathway for the formation of the major phospholipid, phosphatidylethanolamine (PtdEtn). Unlike ChoK, ETNK shows specific activity for its substrate, and displays negligible activity towards N-methylated derivatives of Etn. The Drosophila ETNK is implicated in development and neuronal function. Mammals contain two ETNK proteins, ETNK1 and ETNK2. ETNK1 selectively increases Etn uptake and phosphorylation, as well as PtdEtn synthesis. ETNK2 is found primarily in the liver and reproductive tissues. It plays a critical role in regulating placental hemostasis to support late embryonic development. It may also have a role in testicular maturation. ETNK is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270706 [Multi-domain]  Cd Length: 307  Bit Score: 41.41  E-value: 2.71e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 1359351544 122 VLCHNDfAIY-NIIFN-NEKPVGIIDFDVAAPGPRMWDIA 159
Cdd:cd05157   171 VFCHND-LLYgNILYNeDDDSVTFIDFEYAGPNPRAFDIA 209
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 122-166 2.59e-03

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 38.08  E-value: 2.59e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|
gi 1359351544  122 VLCHNDFAIYNIIFNNEKP-----VGIIDFDVAAPGPRMWDIAYTLYTCV 166
Cdd:smart00587 121 VLNHGDLWANNIMFKYDDEgkpedVALIDFQLSHYGSPAEDLHYFLLTSL 170
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 113-168 3.68e-03

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 38.02  E-value: 3.68e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1359351544 113 MDHTPHNIEVLCHNDFAIYNIIF---NNEKPVGI--IDFDVAAPGPRMWDIAYTLYTCVPL 168
Cdd:pfam02958 206 VEPTPGEFNVLNHGDLWVNNIMFkydDEGEPEDVilVDFQLSRYGSPAIDLNYFLYTSTEL 266
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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