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formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme [Escherichia coli]
Protein Classification
glycyl radical protein ( domain architecture ID 11493154 )
glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase
List of domain hits
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1409.27
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 8 TLS DRI KAH K N AL VHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L A N RT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L E N IP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LA T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVN F P L LL EK GL DG L R EE VAE R RSRINLTVLEDLHGEQ F LK A ID IVL V AV SE HI E R F A A LA R EMAA T ET R ESRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L L EE SER R IRALEESGVYEGEKYS F YQ A AK IVL E AV IN HI L R Y A K LA E EMAA S ET G ESRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE N C DLI A HQP PQTFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYP Y Y RR D VELNQ t L DRE H A I E M L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A AEK PQTFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q nlv D GQPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- D IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G H D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD T VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 647 R ygrgpv GG NYY AG TSSI SANVPFG AQ T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTAAILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 727 L N PATLE N E S DKQKL MIL LR TF F EVH K GW HIQ Y NIV SRE TL LD A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1392738869 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1409.27
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 8 TLS DRI KAH K N AL VHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L A N RT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L E N IP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LA T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVN F P L LL EK GL DG L R EE VAE R RSRINLTVLEDLHGEQ F LK A ID IVL V AV SE HI E R F A A LA R EMAA T ET R ESRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L L EE SER R IRALEESGVYEGEKYS F YQ A AK IVL E AV IN HI L R Y A K LA E EMAA S ET G ESRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE N C DLI A HQP PQTFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYP Y Y RR D VELNQ t L DRE H A I E M L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A AEK PQTFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q nlv D GQPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- D IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G H D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD T VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 647 R ygrgpv GG NYY AG TSSI SANVPFG AQ T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTAAILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 727 L N PATLE N E S DKQKL MIL LR TF F EVH K GW HIQ Y NIV SRE TL LD A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1392738869 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1180.92
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 10 SD RIK AH K NALVHI v KP PVCT ERA QH YTE M Y QQHLD KP IPV RRA L AL A H H L A N R TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 TE RIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L E N A TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H E VC P W W R G Q T VQ DRC YGM F TD E QKGLL A T G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E E IF P Y W K G K T LR DRC FKY F PE E TLIAM A A G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 170 F P LL LEKGLDGL R EE VA E RRSRIN LT VL ED LHGEQ F LK A ID IV LV AV SEHIE R F A A LA R E M AA T ET RES R RD ELL AM AE N 249
Cdd:cd01677 160 Y P KV LEKGLDGL I EE AK E AIEALD LT GP ED IDKIY F YQ A MI IV CE AV ITYAK R Y A E LA K E L AA K ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 250 C DLIAHQ PP Q TFW Q ALQ LCY FI Q LILQIESNGHS V S F GR M DQYLYP Y Y RR D V E LNQ t L D RE H AIE M L HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S P GR F DQYLYP F Y KQ D I E EGR - L T RE G AIE L L ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 330 RSG SHS K AS AG SPLY QN V TIGGQ NL v DG Q pm DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TE - DG S -- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 410 M PAF N NDE I VIP EFIKL G IEPQ DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G H D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK G VSLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 490 S A GN F NN F D E VMD A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT F E E LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 570 G N SLAA V KKLVFE QGAIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD TV D TLLA R A Y QT Y ID E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 650 rgpv GG NY Y A GT S S I SANVPFG AQ T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTAA I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1392738869 730 A TLE N E SDKQ KL MI L L RT F F EV h K G W HIQ Y N I VS R ETL L DA K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 S TLE G E EGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL R DA Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1072.86
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 6 L DTLSD R I K AHKNA L VH i V KP PVCT ERA QHY TE M Y QQHLDK P IPV RRA L A LA H H L ANRT I W IK H DELI I G N Q ASEV R AA P 85
Cdd:COG1882 4 L AGPTE R T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR P 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 86 IFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH E VC P W W R G Q T VQ D RCYGMFTD E QKGLLAT GII K a EGNMTS G DA H 165
Cdd:COG1882 83 IFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR E IA P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG H 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 166 LAVNFPLL L EK GLDGL R EE VA E RRSRIN LT VL ED LHGEQ F L KA ID IV LV AV SEHI ER F A A LARE M A AT ET RES R RD ELL A 245
Cdd:COG1882 162 IIGDYRRV L LY GLDGL I EE AK E KLAELD LT DP ED IEKID F Y KA MI IV CE AV IRLA ER Y A E LARE L A EK ET DPK R KA ELL E 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 246 M AE N C DLIAHQ P PQ TFW Q A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYPYY R RD V E l NQT L DR E H A I E M L HSC W L KL L E 325
Cdd:COG1882 242 I AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S L GR F DQYLYPYY E RD L E - EGR L TE E E A Q E L L DCF W I KL R E 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 326 V NKI R SGSHSKAS AG S P LYQNV TIGG QN l V DG Q pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI R 405
Cdd:COG1882 321 V RFL R TPEYAELF AG Y P TWVTL TIGG MT - P DG R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI S 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 406 C G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G H D ATS GK VFL P Q 485
Cdd:COG1882 398 I G T G S P QYE ND DLM IP MLLNK G VTLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P E 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 486 EKALS ag N F NNF DEVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:COG1882 477 TGDPT -- D F LTY DEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA G 554
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 566 IANLGN SL A A V KKLVF EQGAIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD T VD TLLARAYQ T YI DE LKQ Y HN 645
Cdd:COG1882 555 LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FM DE IRK Y KT 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 646 P R ygrgpv GG N Y YAGTSS I SA NVP F G AQ T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP TAAILG G V LLNQ 725
Cdd:COG1882 632 Y R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP YEKATD G I LLNQ 705
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 726 K LN P AT L EN E SDKQK L MI LLRT F F E v HK G W HIQ Y N I V S RETLLDA K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIAR 805
Cdd:COG1882 706 K FS P SA L GG E EGIEN L VS LLRT Y F D - LG G H HIQ F N V V D RETLLDA Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIAR 784
...
gi 1392738869 806 TEH 808
Cdd:COG1882 785 TEH 787
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
9-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 745.22
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 9 LSD RI KAH K - N ALVHIVK P PVCT ERA QHY TE M Y QQHLDK - P IPV RRA L AL AHH L ANRTIW I KH DELI I G N Q ASEV R AAP I 86
Cdd:pfam02901 1 MWE RI DVL K e N YTLYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 87 F PE YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH E VC P W W R G Q T VQDRCYGMF T D E Q K GLLAT GI IK a EGNMTS G DA H L 166
Cdd:pfam02901 81 Y PE GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR E IF P Y W K G K T HNEGVFDAY T P E M K AARES GI FT - GLPDAY G RG H I 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVNFPLL L EK GLDGL R EE VA E RRSRIN l T VL ED LHGEQ F L KA ID I VLV AV S E HI ER F A A LA R E M A AT ET RES R RD ELL AM 246
Cdd:pfam02901 160 IGDYRRV L LY GLDGL I EE KE E KLAKLD - T DP ED IEKIE F Y KA MI I SCD AV I E YA ER Y A R LA E E L A EQ ET DPK R KA ELL EI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE N C DLIAHQ P PQ TF WQ A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYPYY R RD V E l NQT L DR E H A I E MLHSC W L KL L EV 326
Cdd:pfam02901 239 AE I C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S L GR L DQYLYPYY E RD L E - EGR L TE E E A Q E LIDCF W I KL R EV 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI R SGSHS K AS AG SPLY QN V TIGGQ NL v DG Q pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C 406
Cdd:pfam02901 318 RFL R TPEYN K LF AG YDPF QN L TIGGQ GR - DG R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GG H D ATS GK VFL P Q 485
Cdd:pfam02901 395 G T G S P QYE ND DVM IP ALLNR G VSLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GG R D ELT GK QVG P K 471
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 486 EKALS ag N F NN F D EVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:pfam02901 472 TGPVT -- E F LS F E EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G 549
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 566 I AN LGN SL A A V KKLVF EQGAIGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD T VD TLLARAYQ T YI DE LKQ Y H N 645
Cdd:pfam02901 550 L AN VAD SL S A I KKLVF DDKVYTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FM DE VRK Y K N 627
650 660
....*....|....*....|....*.
gi 1392738869 646 P R ygrgpv GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 628 Y R ------ GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 581.01
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 9 LSD RI KAH K N AL VHIVKP p VCT ERA QH YT EMYQ Q HLDK P IPV RRA L A L A HH L ANRT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL K T AL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E VC - P W W RGQTVQ D RCY G MF TDE Q K GLLA T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E EL f P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVNF P L LL EK GL DG L REEVAER rsrinltv LEDLHGEQ F LK A IDIV L V A VSE HI E R F A A LA RE MAA TE T RES RR D ELL AM 246
Cdd:PRK09983 158 IIDY P R LL NH GL GE L VAQMQQH -------- CQQQPENH F YQ A ALLL L E A SQK HI L R Y A E LA ET MAA NC T DAQ RR E ELL TI 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE NCDLI A HQP PQTFWQA L QL CYFIQL ILQ I ESN GH S V S F GR M DQY LY P Y Y RRD ve L N Q TL D REHAI E M L H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK PQTFWQA C QL FWYMNI ILQ Y ESN AS S L S L GR F DQY ML P F Y QAS -- L T Q GE D PAFLK E L L E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI RS G S HSKAS AG S P LYQNVTI GG qnl VDGQPMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M P AFN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GHDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR G VSLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 484 pqekalsagnfnn FDEVMDAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- YEGLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 564 - V GIANL GN SL A A V K KL VF E Q GAIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D T VD TLL A RAYQT Y ID E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A ELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 643 Y H NPR ygrgpv GG NYYA G TSSI SA N VP F G AQTM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTAAILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 722 LLN Q K LN PATLE N E SDKQ KL MIL LR T F FEV h K GW HIQ Y N I V SRE TL LD A KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT PATLE G E AGLR KL ADF LR A F TQL - K LQ HIQ F N V V NAD TL RE A QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 1392738869 802 II A RT E H M L 810
Cdd:PRK09983 757 II R RT A H Q L 765
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1409.27
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 8 TLS DRI KAH K N AL VHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L A N RT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L E N IP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LA T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVN F P L LL EK GL DG L R EE VAE R RSRINLTVLEDLHGEQ F LK A ID IVL V AV SE HI E R F A A LA R EMAA T ET R ESRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L L EE SER R IRALEESGVYEGEKYS F YQ A AK IVL E AV IN HI L R Y A K LA E EMAA S ET G ESRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE N C DLI A HQP PQTFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYP Y Y RR D VELNQ t L DRE H A I E M L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A AEK PQTFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q nlv D GQPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- D IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G H D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD T VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 647 R ygrgpv GG NYY AG TSSI SANVPFG AQ T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTAAILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 727 L N PATLE N E S DKQKL MIL LR TF F EVH K GW HIQ Y NIV SRE TL LD A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1392738869 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1180.92
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 10 SD RIK AH K NALVHI v KP PVCT ERA QH YTE M Y QQHLD KP IPV RRA L AL A H H L A N R TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 TE RIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L E N A TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H E VC P W W R G Q T VQ DRC YGM F TD E QKGLL A T G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E E IF P Y W K G K T LR DRC FKY F PE E TLIAM A A G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 170 F P LL LEKGLDGL R EE VA E RRSRIN LT VL ED LHGEQ F LK A ID IV LV AV SEHIE R F A A LA R E M AA T ET RES R RD ELL AM AE N 249
Cdd:cd01677 160 Y P KV LEKGLDGL I EE AK E AIEALD LT GP ED IDKIY F YQ A MI IV CE AV ITYAK R Y A E LA K E L AA K ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 250 C DLIAHQ PP Q TFW Q ALQ LCY FI Q LILQIESNGHS V S F GR M DQYLYP Y Y RR D V E LNQ t L D RE H AIE M L HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S P GR F DQYLYP F Y KQ D I E EGR - L T RE G AIE L L ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 330 RSG SHS K AS AG SPLY QN V TIGGQ NL v DG Q pm DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TE - DG S -- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 410 M PAF N NDE I VIP EFIKL G IEPQ DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G H D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK G VSLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 490 S A GN F NN F D E VMD A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT F E E LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 570 G N SLAA V KKLVFE QGAIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD TV D TLLA R A Y QT Y ID E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 650 rgpv GG NY Y A GT S S I SANVPFG AQ T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTAA I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1392738869 730 A TLE N E SDKQ KL MI L L RT F F EV h K G W HIQ Y N I VS R ETL L DA K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 S TLE G E EGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL R DA Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1072.86
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 6 L DTLSD R I K AHKNA L VH i V KP PVCT ERA QHY TE M Y QQHLDK P IPV RRA L A LA H H L ANRT I W IK H DELI I G N Q ASEV R AA P 85
Cdd:COG1882 4 L AGPTE R T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR P 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 86 IFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH E VC P W W R G Q T VQ D RCYGMFTD E QKGLLAT GII K a EGNMTS G DA H 165
Cdd:COG1882 83 IFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR E IA P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG H 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 166 LAVNFPLL L EK GLDGL R EE VA E RRSRIN LT VL ED LHGEQ F L KA ID IV LV AV SEHI ER F A A LARE M A AT ET RES R RD ELL A 245
Cdd:COG1882 162 IIGDYRRV L LY GLDGL I EE AK E KLAELD LT DP ED IEKID F Y KA MI IV CE AV IRLA ER Y A E LARE L A EK ET DPK R KA ELL E 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 246 M AE N C DLIAHQ P PQ TFW Q A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYPYY R RD V E l NQT L DR E H A I E M L HSC W L KL L E 325
Cdd:COG1882 242 I AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S L GR F DQYLYPYY E RD L E - EGR L TE E E A Q E L L DCF W I KL R E 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 326 V NKI R SGSHSKAS AG S P LYQNV TIGG QN l V DG Q pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI R 405
Cdd:COG1882 321 V RFL R TPEYAELF AG Y P TWVTL TIGG MT - P DG R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI S 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 406 C G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G H D ATS GK VFL P Q 485
Cdd:COG1882 398 I G T G S P QYE ND DLM IP MLLNK G VTLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P E 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 486 EKALS ag N F NNF DEVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:COG1882 477 TGDPT -- D F LTY DEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA G 554
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 566 IANLGN SL A A V KKLVF EQGAIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD T VD TLLARAYQ T YI DE LKQ Y HN 645
Cdd:COG1882 555 LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FM DE IRK Y KT 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 646 P R ygrgpv GG N Y YAGTSS I SA NVP F G AQ T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP TAAILG G V LLNQ 725
Cdd:COG1882 632 Y R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP YEKATD G I LLNQ 705
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 726 K LN P AT L EN E SDKQK L MI LLRT F F E v HK G W HIQ Y N I V S RETLLDA K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIAR 805
Cdd:COG1882 706 K FS P SA L GG E EGIEN L VS LLRT Y F D - LG G H HIQ F N V V D RETLLDA Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIAR 784
...
gi 1392738869 806 TEH 808
Cdd:COG1882 785 TEH 787
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
9-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 745.22
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 9 LSD RI KAH K - N ALVHIVK P PVCT ERA QHY TE M Y QQHLDK - P IPV RRA L AL AHH L ANRTIW I KH DELI I G N Q ASEV R AAP I 86
Cdd:pfam02901 1 MWE RI DVL K e N YTLYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 87 F PE YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH E VC P W W R G Q T VQDRCYGMF T D E Q K GLLAT GI IK a EGNMTS G DA H L 166
Cdd:pfam02901 81 Y PE GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR E IF P Y W K G K T HNEGVFDAY T P E M K AARES GI FT - GLPDAY G RG H I 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVNFPLL L EK GLDGL R EE VA E RRSRIN l T VL ED LHGEQ F L KA ID I VLV AV S E HI ER F A A LA R E M A AT ET RES R RD ELL AM 246
Cdd:pfam02901 160 IGDYRRV L LY GLDGL I EE KE E KLAKLD - T DP ED IEKIE F Y KA MI I SCD AV I E YA ER Y A R LA E E L A EQ ET DPK R KA ELL EI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE N C DLIAHQ P PQ TF WQ A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYPYY R RD V E l NQT L DR E H A I E MLHSC W L KL L EV 326
Cdd:pfam02901 239 AE I C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S L GR L DQYLYPYY E RD L E - EGR L TE E E A Q E LIDCF W I KL R EV 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI R SGSHS K AS AG SPLY QN V TIGGQ NL v DG Q pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C 406
Cdd:pfam02901 318 RFL R TPEYN K LF AG YDPF QN L TIGGQ GR - DG R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GG H D ATS GK VFL P Q 485
Cdd:pfam02901 395 G T G S P QYE ND DVM IP ALLNR G VSLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GG R D ELT GK QVG P K 471
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 486 EKALS ag N F NN F D EVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:pfam02901 472 TGPVT -- E F LS F E EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G 549
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 566 I AN LGN SL A A V KKLVF EQGAIGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD T VD TLLARAYQ T YI DE LKQ Y H N 645
Cdd:pfam02901 550 L AN VAD SL S A I KKLVF DDKVYTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FM DE VRK Y K N 627
650 660
....*....|....*....|....*.
gi 1392738869 646 P R ygrgpv GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 628 Y R ------ GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 581.01
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 9 LSD RI KAH K N AL VHIVKP p VCT ERA QH YT EMYQ Q HLDK P IPV RRA L A L A HH L ANRT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL K T AL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E VC - P W W RGQTVQ D RCY G MF TDE Q K GLLA T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E EL f P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 167 AVNF P L LL EK GL DG L REEVAER rsrinltv LEDLHGEQ F LK A IDIV L V A VSE HI E R F A A LA RE MAA TE T RES RR D ELL AM 246
Cdd:PRK09983 158 IIDY P R LL NH GL GE L VAQMQQH -------- CQQQPENH F YQ A ALLL L E A SQK HI L R Y A E LA ET MAA NC T DAQ RR E ELL TI 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 247 AE NCDLI A HQP PQTFWQA L QL CYFIQL ILQ I ESN GH S V S F GR M DQY LY P Y Y RRD ve L N Q TL D REHAI E M L H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK PQTFWQA C QL FWYMNI ILQ Y ESN AS S L S L GR F DQY ML P F Y QAS -- L T Q GE D PAFLK E L L E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 327 NKI RS G S HSKAS AG S P LYQNVTI GG qnl VDGQPMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 407 G F G M P AFN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GHDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR G VSLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 484 pqekalsagnfnn FDEVMDAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- YEGLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 564 - V GIANL GN SL A A V K KL VF E Q GAIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D T VD TLL A RAYQT Y ID E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A ELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 643 Y H NPR ygrgpv GG NYYA G TSSI SA N VP F G AQTM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTAAILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 722 LLN Q K LN PATLE N E SDKQ KL MIL LR T F FEV h K GW HIQ Y N I V SRE TL LD A KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT PATLE G E AGLR KL ADF LR A F TQL - K LQ HIQ F N V V NAD TL RE A QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 1392738869 802 II A RT E H M L 810
Cdd:PRK09983 757 II R RT A H Q L 765
choline_CutC
TIGR04394 choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
9-806
3.12e-144
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain]
Cd Length: 789
Bit Score: 443.86
E-value: 3.12e-144
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 9 LSD R IKAH K NALVH i VK P PVCTE RA QHY TE MYQQHLDK P IPVR RA L A LAHHLANRTIW I KH DELI I G NQASEV RA APIF P 88
Cdd:TIGR04394 1 PTE R LVRL K ENYLK - QV P SITIY RA RAI TE IAKENPGM P KILL RA K A FRKCCETAPLV I QD DELI V G APCGAP RA GAFS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 89 EYTVS W IEK E I D DLAD RP GAG F AV SEE N K RVLH E VC - P W W R G QTVQDR C YGMF td EQK G LLA tgi IKA E GNMTSGDA H l A 167
Cdd:TIGR04394 80 DIAWR W LRD E L D TIGT RP QDP F YI SEE D K KIMR E EI f P F W E G KSLDEY C EDQY -- REA G VWE --- LSG E SFVSDCSY H - A 153
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 168 VN ----- F P ---- L L LE KG LDGLRE E VA E RRSRINLTVL ED LHGEQ F L K AIDIVLVA V SEHIE R FAAL A R E M AA T E TRES 238
Cdd:TIGR04394 154 VN gggds N P gydv I L MK KG MLDIQA E AK E HLAELSYENP ED IDKIY F Y K SVIDTTEG V MIYAK R LSEY A A E L AA K E QDPK 233
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 239 R RD EL LAM AE - N CDLI AH q P P Q TFW Q A L Q LCYFIQLI L QI E S N GHSV S F GR M DQY L YP Y Y RR D V E l NQTLDREH A I E MLH 317
Cdd:TIGR04394 234 R KA EL EKI AE v N ARVP AH - K P R TFW E A I Q SVWTVESL L VV E E N QTGM S I GR V DQY M YP F Y KA D I E - AGRMTEYE A F E LAG 311
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 318 SCWL K LL E VNKIR S GSH SK AS AG SPLYQ N VTI GG QNLVD G qpm DA V N P L S Y AILESCGRLRST QP N L SV R Y H AGMSNDF L 397
Cdd:TIGR04394 312 CMLI K MS E MMWLT S EGG SK FF AG YQPFV N MCV GG VTREG G --- DA T N D L T Y LLMDAVRHVKVY QP S L AC R I H NKSPQKY L 388
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 398 DAC V Q V I R C G F G M PA FNN D EIV I PEFIKL G IEPQ DA Y DY AAI GC I E TAVG G KW g Y RC T GMSFINFARVMLAA L EG G HDAT 477
Cdd:TIGR04394 389 KKI V D V V R A G M G F PA CHF D DAH I KMMLAK G VSIE DA R DY CLM GC V E PQKS G RL - Y QW T STAYTQWPICIELV L NH G VPLW 467
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 478 S GK VFL P QEKA LS ag N F NNFDEVMD A WDT QI R Y Y T RK S ieiey V V D T MLEEN VH - D I ---- L C S ALVDD C I E RA K SIKQ G 552
Cdd:TIGR04394 468 Y GK QVC P DTGD LS -- Q F DTYEKFDA A VKE QI K Y I T KW S ----- A V A T VISQR VH r D L apkp L M S LMYEG C M E KG K DVSA G 540
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 553 GA K Y DWVS G L - QV G I A NLGN S L AA V KKLV FEQGAIGQQ QL AA AL ADD F D G lt H EQ L R QRLIN g APKYGNDDD TV D TLL A - 630
Cdd:TIGR04394 541 GA M Y NFGP G V v WS G L A TYAD S M AA I KKLV YDDKKYTLE QL NE AL KAN F E G -- Y EQ I R ADCLD - APKYGNDDD YA D LIA A d 617
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 631 --------- R A Y Q T YIDE L KQ yhnprygrgpvggnyya GT S SIS A N V PFG AQ T M A TPD GR K A H TPL AE G A SP AS G T D HL G 701
Cdd:TIGR04394 618 lvnftereh R K Y K T LYSH L SH ----------------- GT L SIS N N T PFG QL T G A SAN GR L A W TPL SD G I SP TQ G A D FK G 680
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 702 PTA V I G SV G K LPTAAILG G VLL N Q KL NPAT L ENESDKQK L MI LLRT FFEVHK G w HI Q Y N IVSR ETLLDA KK HP DQ YRDLV 781
Cdd:TIGR04394 681 PTA I I K SV S K MANDSMNI G MVH N F KL MSGL L DTPEGENG L IT LLRT ASILGN G - EM Q F N YLDN ETLLDA QQ HP EK YRDLV 759
810 820
....*....|....*....|....*
gi 1392738869 782 VRVAGYSAFF TA L SP D A QD D II A RT 806
Cdd:TIGR04394 760 VRVAGYSAFF VE L CK D V QD E II S RT 784
PFL1
cd01678 Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
68-808
1.55e-59
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain]
Cd Length: 738
Bit Score: 215.69
E-value: 1.55e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 68 K HD E L I I G N Q ASEVRAAP I F P EYTVSWI E KEIDD ladrpg A G FAVSE E N K RVLHE vcpw W R g Q T VQ D RCYGMF T D E QKGL 147
Cdd:cd01678 82 K EL E V I V G L Q TDKPLKRA I M P FGGIRMA E QALKA ------ Y G YELDP E L K KIFTK ---- Y R - K T HN D GVFDAY T P E IRRA 150
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 148 LAT GII K ------ AE G NMTSGDAHL A V nfpllle K G L D G L R EE VAE rrsrinltvle DL HGEQFLKAI D IVLVAVS E HI E 221
Cdd:cd01678 151 RHS GII T glpday GR G RIIGDYRRV A L ------- Y G V D R L I EE KKK ----------- DL DNLGGDEMT D DTIRLRE E VA E 212
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 222 RFA AL aremaatetresrr D EL LA MA ENCD L IAHQ P PQTFWQ A L Q LC YF IQ L ILQI E S NG HSV S F GR MDQY L YP Y YR RD V 301
Cdd:cd01678 213 QIK AL -------------- K EL KQ MA ASYG L DISR P ATNAQE A I Q WT YF GY L AAIK E Q NG AAM S L GR VSTF L DI Y IE RD L 278
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 302 E l NQ T LDREH A I E MLHSCWL KL LE V NKI R SGSHSKASA G S P LYQNVT IGG QNL v DG QPM da V NPL S YAI L ESCGR L - RST 380
Cdd:cd01678 279 K - AG T ITEAE A Q E LIDQFIM KL RM V RFL R TPEYNELFS G D P TWVTES IGG MGN - DG RSL -- V TKT S FRF L NTLYN L g PAP 354
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 381 Q PNL S V RYHAGMSND F LDA C VQ V ------ I R cgfgmpa FN ND EIVI P EF iklgiepqd AY D YAA I G C IET A VG - GK ---- 449
Cdd:cd01678 355 E PNL T V LWSEKLPEN F KRF C AK V sidtss I Q ------- YE ND DLMR P DW --------- GG D DYG I A C CVS A MR i GK qmqf 418
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 450 W G Y R C tgmsfi N F A RVM L A A LE GG H D ATS G KVFL P QEKAL s AGNFNNF DEVM DAW D TQIRYYTRKSIEIEYVVDT M LEEN 529
Cdd:cd01678 419 F G A R A ------ N L A KAL L Y A IN GG R D EKT G DQVG P DIEPI - TSDYLDY DEVM ENY D KSMDWLADTYVNALNIIHY M HDKY 491
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 530 VHDI L CS AL V D DCIE R A ksikqggakydwvsg LQV GIA N L GN --- SL A A V K -- K LVFEQGAI G qqqlaaa LA D DF D glth 604
Cdd:cd01678 492 AYEA L QM AL H D TDVR R T --------------- MAF GIA G L SV aad SL S A I K ya K VKPIRDED G ------- LA V DF E ---- 545
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 605 eqlrqrl I N G A - P K YGNDDD TV D TLLARAYQ T YIDE L KQYHNP R ygrgpvgg N YYAGT S -- S I SA NV PF G AQ T MA TPDGR 681
Cdd:cd01678 546 ------- I E G D f P R YGNDDD RA D DIAVWVVK T FMNK L RKHKTY R -------- N AEPTQ S vl T I TS NV VY G KK T GN TPDGR 610
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 682 K A HT P L A E GA S P AS G T D HL G PT A VIG SV G KLP TAAILG G VLLNQKLN P AT L ENESDKQK -- L MIL L RTF F e VHK G W H IQY 759
Cdd:cd01678 611 R A GE P F A P GA N P MH G R D KK G AL A SLA SV A KLP YRDAND G ISNTFSIV P NA L GKTDEERI dn L VGI L DGY F - TKG G H H LNV 689
730 740 750 760
....*....|....*....|....*....|....*....|....*....
gi 1392738869 760 N IVS RETLLDA KK HP DQ Y RD L VV RV A GY SAF F TA L SPDA Q D D I I A RT E H 808
Cdd:cd01678 690 N VLN RETLLDA ME HP EK Y PQ L TI RV S GY AVN F VK L TREQ Q L D V I S RT F H 738
Gly_radical
pfam01228 Glycine radical;
687-792
5.31e-41
Glycine radical;
Pssm-ID: 426140 [Multi-domain]
Cd Length: 106
Bit Score: 145.39
E-value: 5.31e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 687 L A E G A SP AS G T D HL GPTAV IG SVGK LPTAAI L G G VL LNQK LN PA T L ENESDKQ -- K L MI L LR T F F ev HK G W H I Q Y N I V S R 764
Cdd:pfam01228 1 V A P G I SP SH G A D FE GPTAV LN SVGK IDYEVE L D G IS LNQK FL PA V L GYYDEEG ya N L NT L ID T Y F -- EG G H H L Q F N V V D R 78
90 100
....*....|....*....|....*...
gi 1392738869 765 ETL L DA K KHP DQ Y R DL V VRV A GYSA F F T 792
Cdd:pfam01228 79 ETL P DA Q KHP EK Y P DL T VRV S GYSA N F V 106
PRK11127
PRK11127 autonomous glycyl radical cofactor GrcA; Provisional
751-808
3.55e-16
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain]
Cd Length: 127
Bit Score: 75.65
E-value: 3.55e-16
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 1392738869 751 V HK G W H IQY N IVS RETL L DA K KHP DQ Y RD L VV RV A GY SAF F TA L S P DA Q D D I IART EH 808
Cdd:PRK11127 67 V EG G Q H LNV N VLR RETL E DA V KHP EK Y PQ L TI RV S GY AVR F NS L T P EQ Q R D V IART FT 124
RNR_PFL
cd00576 Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
242-467
2.15e-13
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain]
Cd Length: 401
Bit Score: 72.95
E-value: 2.15e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 242 ELLAMAENCDLIAHQPPQTFWQ A L Q LC Y F I QLILQIES NG HS VSF G R MDQY L Y PY YR RD VEL n QTLDREH A I E MLHSCW L 321
Cdd:cd00576 29 DYGDSLDPGIKGVNETAKSINE A I Q KT Y Q I IALAASNQ NG GG VSF A R ASSI L S PY GS RD YAK - GSGTETD A V E AADAFN L 107
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 322 K L L EV NK irsgshska SA G SPLYQNVT IGG QNL v DGQPMDAVNP L SY A ILESCGRLRSTQ PNLSVR YHAGMSN ------ D 395
Cdd:cd00576 108 A L K EV GQ --------- GN G RTGAATGF IGG VHK - GKGDKISQEF L NL A LANGGEGIPLNF PNLSVR VSSDKPG ilvkav E 177
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1392738869 396 FLDACVQVI R c GF G M P AFN NDE I -------------- V I P ----------- E FIK L GIEPQ D AYDYAAIGC I E T AVG G KW 450
Cdd:cd00576 178 LKQLIAEEA R - KT G S P GIF NDE L cnlvslnlarimek A I N gsmdvvleele E LAF L AVRAL D CVIDSHDER I P T IEL G GD 256
250
....*....|....*..
gi 1392738869 451 GY R CT G MSFINF A RVML 467
Cdd:cd00576 257 ER R TV G LGIAGV A DLLI 273
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
