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Conserved domains on  [gi|1400582494|gb|PYP97778|]
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succinylglutamate desuccinylase [Gemmatimonadetes bacterium]

Protein Classification

M14 family metallopeptidase( domain architecture ID 13031228)

M14 family metallopeptidase is a zinc-binding carboxypeptidase which hydrolyzes a single, C-terminal amino acid from a polypeptide chain, and has a recognition site for the free C-terminal carboxyl group

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 71-258 1.45e-95

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


:

Pssm-ID: 349484  Cd Length: 187  Bit Score: 281.82  E-value: 1.45e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  71 LALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYSPIDGKNLNRVYPGKADGTVSERIAYAI 150
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 151 TNQIIERADYLVDIHGGDGNESLRPYSYWSPLGlNARADSIAREMALAWGSDHIVVDTMRPRDVRASVYTQNTAQLRGKP 230
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETG-NAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIP 159

                         170       180
                  ....*....|....*....|....*...
gi 1400582494 231 SITTETGYLGIPAEDMVQRNVDGVFRLL 258
Cdd:cd18174   160 AILVEAGGLGSRDEEDVARHVEGVLNVL 187
 
Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 71-258 1.45e-95

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 281.82  E-value: 1.45e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  71 LALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYSPIDGKNLNRVYPGKADGTVSERIAYAI 150
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 151 TNQIIERADYLVDIHGGDGNESLRPYSYWSPLGlNARADSIAREMALAWGSDHIVVDTMRPRDVRASVYTQNTAQLRGKP 230
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETG-NAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIP 159

                         170       180
                  ....*....|....*....|....*...
gi 1400582494 231 SITTETGYLGIPAEDMVQRNVDGVFRLL 258
Cdd:cd18174   160 AILVEAGGLGSRDEEDVARHVEGVLNVL 187
COG3608 COG3608
Predicted deacylase [General function prediction only];
51-348 5.40e-84

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 256.70  E-value: 5.40e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  51 SDAATRIPITIVRGAQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTiYYSPIDGKNL 130
Cdd:COG3608     9 SGTPVSLPVTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRELDPGELRGTVILVPVANPPGFLQGS-RYLPIDGRDL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 131 NRVYPGKADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYWSPLglnaraDSIAREMALAWGSDHIVVDTMR 210
Cdd:COG3608    88 NRSFPGDADGSLAERIAHALFEEILPDADYVIDLHSGGIARDNLPHVRAGPG------DEELRALARAFGAPVILDSPEG 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 211 PRDVRAsvytqNTAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMVPGDVELV--RQPLFFERTEVLRSPAT 288
Cdd:COG3608   162 GDGSLR-----EAAAEAGIPALTLELGGGGRFDEESIEAGVRGILNVLRHLGMLDGEAPPPplAPPVLARGSEWVRAPAG 236

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 289 GVWHARVERGQSVQKGSVIGVLTDFFGNPQAEIRAPFGGIVLYVVATPAMSQGEPMGMIG 348
Cdd:COG3608   237 GLFEPLVELGDRVKKGDVLGRITDPFGEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 67-344 1.19e-30

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 117.84  E-value: 1.19e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  67 SGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYyspIDGkNLNRVYPGKADG------ 140
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRY---IPR-DLNRSFPGRALGassdep 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 141 ---TVSERIAYAITNQIIERADYLVDIHGGDGNeslrpySYWSPLGLNARADsiAREMALAW----GSDHIVVDTMRPRD 213
Cdd:pfam04952  77 yraTRAERLADLFFPALLPRADIVLDLHTGTRG------MGHLLFALAPIRD--DPLHLLALlrafGAPAVLKLHSKPSA 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 214 VRASVYTQNTaqlrGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMVPGDVELVRQPLFFE------RTEVLRSPA 287
Cdd:pfam04952 149 GFSAFSAEEL----GAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPPKLYRvlreidRPRDIRAEL 224

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1400582494 288 TGVWHARVERGQSVQKGSVIGVLTDF--FGNPQAEIRAPFGGIVLYVVATPAMSQGEPM 344
Cdd:pfam04952 225 AGLVEFALNLGDDVDAGPLLPGGPLFapFGGEETEYRAPEDGYPVFPNEAAYVGKGAAL 283
 
Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 71-258 1.45e-95

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 281.82  E-value: 1.45e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  71 LALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYSPIDGKNLNRVYPGKADGTVSERIAYAI 150
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 151 TNQIIERADYLVDIHGGDGNESLRPYSYWSPLGlNARADSIAREMALAWGSDHIVVDTMRPRDVRASVYTQNTAQLRGKP 230
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETG-NAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIP 159

                         170       180
                  ....*....|....*....|....*...
gi 1400582494 231 SITTETGYLGIPAEDMVQRNVDGVFRLL 258
Cdd:cd18174   160 AILVEAGGLGSRDEEDVARHVEGVLNVL 187
COG3608 COG3608
Predicted deacylase [General function prediction only];
51-348 5.40e-84

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 256.70  E-value: 5.40e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  51 SDAATRIPITIVRGAQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTiYYSPIDGKNL 130
Cdd:COG3608     9 SGTPVSLPVTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRELDPGELRGTVILVPVANPPGFLQGS-RYLPIDGRDL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 131 NRVYPGKADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYWSPLglnaraDSIAREMALAWGSDHIVVDTMR 210
Cdd:COG3608    88 NRSFPGDADGSLAERIAHALFEEILPDADYVIDLHSGGIARDNLPHVRAGPG------DEELRALARAFGAPVILDSPEG 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 211 PRDVRAsvytqNTAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMVPGDVELV--RQPLFFERTEVLRSPAT 288
Cdd:COG3608   162 GDGSLR-----EAAAEAGIPALTLELGGGGRFDEESIEAGVRGILNVLRHLGMLDGEAPPPplAPPVLARGSEWVRAPAG 236

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 289 GVWHARVERGQSVQKGSVIGVLTDFFGNPQAEIRAPFGGIVLYVVATPAMSQGEPMGMIG 348
Cdd:COG3608   237 GLFEPLVELGDRVKKGDVLGRITDPFGEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
M14_ASTE_ASPA-like cd06254
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 58-261 6.49e-81

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349472  Cd Length: 198  Bit Score: 245.18  E-value: 6.49e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  58 PITIVRGAQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYSPIDGKNLNRVYPGK 137
Cdd:cd06254     1 PVTLINGAKPGPTLLITAGIHGGEYPGILAAIRLARELDPADVKGTLIIVHIANVSGFEARTPFVVPEDGKNLNRVFPGD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 138 ADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYWsPLGLNARADSIAREMALAWGSDHIVVDTMrprdvRAS 217
Cdd:cd06254    81 PDGTLTERIAYFLTREIISRADFLIDLHGGDANEALTPFVYY-PGGASEEVNDISRAAAQALGLPYIVISSS-----EKG 154

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 1400582494 218 VYTQNTAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYF 261
Cdd:cd06254   155 TGYYSYAALRGIPSILVERGGLGTCDEEDVQAHKDGIKNLLRHL 198
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 57-264 1.45e-48

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 161.94  E-value: 1.45e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  57 IPITIVRGAQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYsPIDGKNLNRVYPG 136
Cdd:cd06251     1 VPVLVARGAKPGPTLLLTAAIHGDELNGIEVIQRLLEDLDPSKLRGTLIAIPVVNPLGFENNSRYL-PDDGRDLNRSFPG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 137 KADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYwsplgLNARADSIaREMALAWGSDHIVVDTMRPRDVRa 216
Cdd:cd06251    80 SEKGSLASRLAHLLWNEIVKKADYVIDLHTASTGRTNLPYVR-----ADLRDPES-RRMAEAFGAPVIVDDPGEDGSLR- 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1400582494 217 svytqNTAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMV 264
Cdd:cd06251   153 -----GAAVELGIPAITVELGEALRFDEDIIRRGVEGVLNVLRHLGML 195
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 71-257 8.83e-41

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 141.30  E-value: 8.83e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  71 LALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTiYYSPIDGKNLNRVYPGKADGTVSERIAYAI 150
Cdd:cd06230     1 LLILAGVHGDEYEGVEAIRRLLAELDPSELKGTVVLVPVANPPAFEAGT-RYTPLDGLDLNRIFPGDPDGSPTERLAHEL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 151 TNQIIERADYLVDIHGGdGNESLRPYSYwspLGLNARADSIAREMALAWGSDHIVVDtmrpRDVRASVyTQNTAQLRGKP 230
Cdd:cd06230    80 TELILKHADALIDLHSG-GTGRLVPYAI---LDYDSDAREKSRELARAFGGTPVIWG----GDPPGGT-PVAAARSAGIP 150

                         170       180
                  ....*....|....*....|....*..
gi 1400582494 231 SITTETGYLGIPAEDMVQRNVDGVFRL 257
Cdd:cd06230   151 AITVELGGGGRLRAERLERYLRGIRNV 177
M14_ASTE_ASPA-like cd06252
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 41-264 6.63e-40

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349470  Cd Length: 224  Bit Score: 140.40  E-value: 6.63e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  41 SSGFIEVPAGSD----AATRIPITIVRGaQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFL 116
Cdd:cd06252     4 QAGFLRLPLSDDrsawGAIPIPITVINN-GSGPTVLLTGGNHGDEYEGPIALRRLARDLDPEDVRGRLIIVPALNLPAVR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 117 G--RTiyySPIDGKNLNRVYPGKADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYWSPLGlNARADSIARE 194
Cdd:cd06252    83 AgtRT---SPLDGGNLNRAFPGDADGTPTERIAHFLETVLLPRADAVIDLHSGGSSLDFVPCAAVHLLP-DPAQRARSLA 158

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 195 MALAWGSDHIVVdtmrPRDVRASVYTQNTAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMV 264
Cdd:cd06252   159 LAEAFGAPLSVV----VDNVDAPGTLDSAAERAGKIFVSTELGGGGTVTPAALRIAERGVLNVLIHLGVL 224
M14_ASTE_ASPA-like cd06255
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 51-263 8.36e-36

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349473  Cd Length: 223  Bit Score: 129.75  E-value: 8.36e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  51 SDAATRIPITIVRGAQSGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTiYYSPIDGKNL 130
Cdd:cd06255     6 SGAPVTIPVIVVRGAKPGPCLWINGAVHGDELNGPLAALELFRELDPAQLSGTLVATPIANPLAFQGRQ-KFSPQDGEDL 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 131 NRVYPGKADGTVSERIAYAITNQIIERADYLVDIHGGDGNESLRPYSYWS-PLGLNARADSIAREMALAWG-SDHIVVDT 208
Cdd:cd06255    85 DQSFPGDPDGLITERMAHALFSEVKEVADYLIDFHTGGTPFDANPYTVYKlFPESGPVEEKRLLRLARAFGvHANCRVDV 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1400582494 209 MRPRDVRASVYTQN---TAQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHM 263
Cdd:cd06255   165 SGAGGELPGNTAGAldyQCMAQGIPAFMVELGGGGRAEEEAVRFAARGLRNLLRYLGM 222
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 67-344 1.19e-30

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 117.84  E-value: 1.19e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  67 SGPTLALIAGTHGSEVAPIVALQRVRAIIDPAVLRGTLLIVHVANMPSFLGRTIYyspIDGkNLNRVYPGKADG------ 140
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRY---IPR-DLNRSFPGRALGassdep 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 141 ---TVSERIAYAITNQIIERADYLVDIHGGDGNeslrpySYWSPLGLNARADsiAREMALAW----GSDHIVVDTMRPRD 213
Cdd:pfam04952  77 yraTRAERLADLFFPALLPRADIVLDLHTGTRG------MGHLLFALAPIRD--DPLHLLALlrafGAPAVLKLHSKPSA 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 214 VRASVYTQNTaqlrGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYFHMVPGDVELVRQPLFFE------RTEVLRSPA 287
Cdd:pfam04952 149 GFSAFSAEEL----GAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPPKLYRvlreidRPRDIRAEL 224

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1400582494 288 TGVWHARVERGQSVQKGSVIGVLTDF--FGNPQAEIRAPFGGIVLYVVATPAMSQGEPM 344
Cdd:pfam04952 225 AGLVEFALNLGDDVDAGPLLPGGPLFapFGGEETEYRAPEDGYPVFPNEAAYVGKGAAL 283
M14_ASTE_ASPA-like cd06253
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 64-261 7.81e-18

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349471  Cd Length: 211  Bit Score: 80.72  E-value: 7.81e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  64 GAQSGPTLALIAGTHGSEVAPI-VALQRVRAI----IDPAVLRGTLLIVHVANMPSFLGRTiYYSPIDGKNLNRVYPGKA 138
Cdd:cd06253    18 GGNAEPRIAIVAGIHGDELNGLyVCSRLIRFLkeleEGGYKLKGKVLVIPAVNPLGINSGT-RFWPFDNLDMNRMFPGYN 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 139 DGTVSERIAYAITnQIIERADYLVDIHGGDGneslrPYSYWSPLGLNARADSIAREMALAWGSDHIVVDTMRPrdVRASV 218
Cdd:cd06253    97 KGETTERIAAALF-EDLKGADYGIDLHSSND-----FLREIPQVRVIESGAQDLLPLAKFLGLDVVWVHPAST--VDTGT 168

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1400582494 219 YTQNTaQLRGKPSITTETGYLGIPAEDMVQRNVDGVFRLLRYF 261
Cdd:cd06253   169 LAYNW-NEWGTKALVLEMGVGMRIDKEYCEQLFEGILRFLLKM 210
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 71-243 5.18e-07

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 48.98  E-value: 5.18e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  71 LALIAGTHGSEVAPIVALQRVRAIID-PAVLRGTLLIVhVANMPSFLGRTIYyspIDgKNLNRVYPGKADGTVSE-RIAY 148
Cdd:cd18430     1 LAVLGAVHGNETCGTRAVERLLAELPsGALQKGPVTLV-PANERAYAEGVRF---CE-EDLNRVFPGDPDPDTYErRLAN 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 149 AITnQIIERADYLVDIH----GGDgneslrPYSYWSPlgLNARADSIAREMAlawgsdhivvdtmrPRDVRASVYT--QN 222
Cdd:cd18430    76 RLC-PELEGHDVVLDLHsthsGGQ------PFAILDY--GDKASRRLARSVG--------------IPKGWRVVYGrdLG 132

                         170       180
                  ....*....|....*....|....
gi 1400582494 223 TAQLRGKPSITT---ETGYLGIPA 243
Cdd:cd18430   133 YAHGGGKTEVTGvtvECGYHDSEE 156
M14_CP_Csd4-like cd06243
Peptidase M14 carboxypeptidase Csd4 and similar proteins; This family includes peptidase M14 ...
 58-169 7.03e-05

Peptidase M14 carboxypeptidase Csd4 and similar proteins; This family includes peptidase M14 carboxypeptidase Csd4 from H. pylori which has been shown to be DL-carboxypeptidase with a modified zinc binding site containing a glutamine residue in place of a conserved histidine. It is an archetype of a new carboxypeptidase subfamily with a domain arrangement that differs from this family of peptide-cleaving enzymes. Csd4 plays a role in trimming uncrosslinked peptidoglycan peptide chains by cleaving the amide bond between meso-diaminopimelate and iso-D-glutamic acid in truncated peptidoglycan side chains. It acts as a cell shape determinant, similar to Campylobacter jejuni Pgp1. The M14 family of metallocarboxypeptidases (MCPs), also known as funnelins, are zinc-binding carboxypeptidases (CPs) which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Two major subfamilies of the M14 family, defined based on sequence and structural homology, are the A/B and N/E subfamilies. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide, followed by an N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity; Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. The A forms have slightly different specificities, with Carboxypeptidase A1 (CPA1) preferring aliphatic and small aromatic residues, and CPA2 preferring the bulky aromatic side chains. Enzymes belonging to the N/E subfamily enzymes are not produced as inactive precursors and instead rely on their substrate specificity and subcellular compartmentalization to prevent inappropriate cleavage. They contain an extra C-terminal transthyretin-like domain, thought to be involved in folding or formation of oligomers. MCPs can also be classified based on their involvement in specific physiological processes; the pancreatic MCPs participate only in alimentary digestion and include carboxypeptidase A and B (A/B subfamily), while others, namely regulatory MCPs or the N/E subfamily, are involved in more selective reactions, mainly in non-digestive tissues and fluids, acting on blood coagulation/fibrinolysis, inflammation and local anaphylaxis, pro-hormone and neuropeptide processing, cellular response and others. Another MCP subfamily, is that of succinylglutamate desuccinylase /aspartoacylase, which hydrolyzes N-acetyl-L-aspartate (NAA), and deficiency in which is the established cause of Canavan disease. Another subfamily (referred to as subfamily C) includes an exceptional type of activity in the MCP family, that of dipeptidyl-peptidase activity of gamma-glutamyl-(L)-meso-diaminopimelate peptidase I which is involved in bacterial cell wall metabolism.


Pssm-ID: 349462  Cd Length: 227  Bit Score: 43.50  E-value: 7.03e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  58 PITIVRGAQSGPTLALIAGTHGSEVAPIVALQRvraIIDPAVLRGTLLIVHVANMPSFLGRTIYYspiDGkNLNR----V 133
Cdd:cd06243     6 PFTRLEGREPGPTLLIIGGIQGDEPGGFLAADL---LADLYLVKGNVIVVPRLNFPSILRNHRGL---NG-DMNRkfaaL 78

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1400582494 134 YPGKADGTVSERIAYAITNqiiERADYLVDIHGGDG 169
Cdd:cd06243    79 DKKDPEYKTIQEIKSLIAD---FRPDVVLHLHDGSG 111
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 66-237 4.73e-03

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 37.71  E-value: 4.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  66 QSGPTLALIAGTHGSEVAPIVALQR-VRAIIDPAVLRGTLLIVHVANMPSFLGRTIYYSPIDGKNLNRVY-PGKADG--- 140
Cdd:cd06910    22 QPGPHVMINALTHGNEICGAIALDWlLKNGVRPLRGRLTFCFANVEAYERFDPARPTASRFVDEDLNRVWgPELLDGpeq 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 141 TVSERIAYAItNQIIERADYLVDIHggDGNESLRPYSYWSPLGLNaradsiaREMALAWGS-DHIVVDTMRPRDVRASVY 219
Cdd:cd06910   102 SIELRRAREL-RPVVDTVDYLLDIH--SMQEKVPPLALAGDKEKG-------RALARRVGSpAHLLIDAGHAEGLRLRDY 171

                         170
                  ....*....|....*...
gi 1400582494 220 TQNTAQLRGKPSITTETG 237
Cdd:cd06910   172 AGFGDPSSPKNALLVECG 189
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
65-165 4.96e-03

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 38.29  E-value: 4.96e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  65 AQSGPTLALIAGTHGSEVAPIVALQR-VRAIidpavLRGTL-----LIVHVANMPSFLGRTIYyspIDgKNLNRVYPG-- 136
Cdd:COG2988    21 APGIKAVVISGGIHGNETAPIELLDKlLQDL-----LLGERplsfrLLLILGNPAAMRAGRRY---LD-EDLNRLFGGrh 91

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1400582494 137 -KADGTVSERIAYAItNQIIE-------RADYLVDIH 165
Cdd:COG2988    92 lQNPESYEAARAKEL-EQAVGpffaaggRVRLHIDLH 127
M14_PaAOTO_like cd06250
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like subfamily; ...
 45-195 7.51e-03

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like subfamily; subgroup includes Pseudomonas aeruginosa AotO; An uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the the M14 family of metallocarboxypeptidases. This subgroup includes Pseudomonas aeruginosa AotO and related proteins. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD. The gene encoding P. aeruginosa AotO was characterized as part of an operon encoding an arginine and ornithine transport system, however it is not essential for arginine and ornithine uptake.


Pssm-ID: 349468  Cd Length: 267  Bit Score: 37.60  E-value: 7.51e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494  45 IEVPAGSDAaTRIPITIVR--GAQSGPTLALIAGTHGSEVAPIVALQ----RVRAIIDPAVLRGTLLIVHVAN---MPSF 115
Cdd:cd06250     3 IPLDSPAPG-TERSLTVFRfgGAGAGPKVYIQAALHADELPGNLVIHhlleRLKALEAAGRIKGEIVLVPQANpigLSQK 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1400582494 116 LGRTIY--YSPIDGKNLNRVYP--------------GKADGTVSERIAYAITNQIIER-------------------ADY 160
Cdd:cd06250    82 IGGYHQgrFDLATGDNFNRNFPdlakavaarveerlGDDAAANVALIRAALKEALDALpprtelqrlkltllrlaldADI 161

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1400582494 161 LVDIHGGDgnESLRpYSYwSPLGLNARADSIAREM 195
Cdd:cd06250   162 VLDLHCDD--EALR-HLY-TPPALWPAAEDLAAAL 192
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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