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Conserved domains on  [gi|74629021|sp|Q7SDY6|]
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RecName: Full=Pre-mRNA-splicing factor slu-7

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 137-378 6.83e-102

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


:

Pssm-ID: 463326  Cd Length: 258  Bit Score: 303.03  E-value: 6.83e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   137 LGYEAKRDVYSAYDPKQYMEVVEEYNMLEEARRALQGDQKTPDGEGADGPED--------DKSGFKYDEESDMGRDRATT 208
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEKDDEGDDDdseeedndDEDEDKYADEADMGGQKVDS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   209 KQS-----MRIREDTAKYLLNLDSDSAKYNPKKRALVDAGAI---ADKSAALFAEESFLRASGEAAEFEKAQRYAWEAQE 280
Cdd:pfam11708  81 KQRttvrnLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPnvdPSEDKALFAGDNFVRASGDAAEFEKLQRFAWEAQE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   281 RSGDtsLHLQANPTAGEILRKKESEEREAKRRKRAEELANQYGTQPVISDALRET-IKESETFVEYDEAG-LIKGAPKKV 358
Cdd:pfam11708 161 KGDD--VHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELlLAQTERYVEYDRSGrVIKGQEKAK 238

                         250       260
                  ....*....|....*....|
gi 74629021   359 GKSKYLEDVYINNHTSVWGS 378
Cdd:pfam11708 239 AKSKYEEDVYINNHTSVWGS 258
PTZ00368 super family cl31762
universal minicircle sequence binding protein (UMSBP); Provisional
 96-149 9.97e-03

universal minicircle sequence binding protein (UMSBP); Provisional


The actual alignment was detected with superfamily member PTZ00368:

Pssm-ID: 173561 [Multi-domain]  Cd Length: 148  Bit Score: 36.32  E-value: 9.97e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 74629021   96 ACENCGAMGHKKKDCLERPRKFGAKATGKDIQADRIVRDVKLGYEAKRDVYSAY 149
Cdd:PTZ00368  54 SCYNCGKTGHLSRECPEAPPGSGPRSCYNCGQTGHISRECPNRAKGGAARRACY 107
 
Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 137-378 6.83e-102

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


Pssm-ID: 463326  Cd Length: 258  Bit Score: 303.03  E-value: 6.83e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   137 LGYEAKRDVYSAYDPKQYMEVVEEYNMLEEARRALQGDQKTPDGEGADGPED--------DKSGFKYDEESDMGRDRATT 208
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEKDDEGDDDdseeedndDEDEDKYADEADMGGQKVDS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   209 KQS-----MRIREDTAKYLLNLDSDSAKYNPKKRALVDAGAI---ADKSAALFAEESFLRASGEAAEFEKAQRYAWEAQE 280
Cdd:pfam11708  81 KQRttvrnLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPnvdPSEDKALFAGDNFVRASGDAAEFEKLQRFAWEAQE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   281 RSGDtsLHLQANPTAGEILRKKESEEREAKRRKRAEELANQYGTQPVISDALRET-IKESETFVEYDEAG-LIKGAPKKV 358
Cdd:pfam11708 161 KGDD--VHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELlLAQTERYVEYDRSGrVIKGQEKAK 238

                         250       260
                  ....*....|....*....|
gi 74629021   359 GKSKYLEDVYINNHTSVWGS 378
Cdd:pfam11708 239 AKSKYEEDVYINNHTSVWGS 258
PTZ00368 PTZ00368
universal minicircle sequence binding protein (UMSBP); Provisional
 96-149 9.97e-03

universal minicircle sequence binding protein (UMSBP); Provisional


Pssm-ID: 173561 [Multi-domain]  Cd Length: 148  Bit Score: 36.32  E-value: 9.97e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 74629021   96 ACENCGAMGHKKKDCLERPRKFGAKATGKDIQADRIVRDVKLGYEAKRDVYSAY 149
Cdd:PTZ00368  54 SCYNCGKTGHLSRECPEAPPGSGPRSCYNCGQTGHISRECPNRAKGGAARRACY 107
 
Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 137-378 6.83e-102

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


Pssm-ID: 463326  Cd Length: 258  Bit Score: 303.03  E-value: 6.83e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   137 LGYEAKRDVYSAYDPKQYMEVVEEYNMLEEARRALQGDQKTPDGEGADGPED--------DKSGFKYDEESDMGRDRATT 208
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEKDDEGDDDdseeedndDEDEDKYADEADMGGQKVDS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   209 KQS-----MRIREDTAKYLLNLDSDSAKYNPKKRALVDAGAI---ADKSAALFAEESFLRASGEAAEFEKAQRYAWEAQE 280
Cdd:pfam11708  81 KQRttvrnLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPnvdPSEDKALFAGDNFVRASGDAAEFEKLQRFAWEAQE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74629021   281 RSGDtsLHLQANPTAGEILRKKESEEREAKRRKRAEELANQYGTQPVISDALRET-IKESETFVEYDEAG-LIKGAPKKV 358
Cdd:pfam11708 161 KGDD--VHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELlLAQTERYVEYDRSGrVIKGQEKAK 238

                         250       260
                  ....*....|....*....|
gi 74629021   359 GKSKYLEDVYINNHTSVWGS 378
Cdd:pfam11708 239 AKSKYEEDVYINNHTSVWGS 258
PTZ00368 PTZ00368
universal minicircle sequence binding protein (UMSBP); Provisional
 96-149 9.97e-03

universal minicircle sequence binding protein (UMSBP); Provisional


Pssm-ID: 173561 [Multi-domain]  Cd Length: 148  Bit Score: 36.32  E-value: 9.97e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 74629021   96 ACENCGAMGHKKKDCLERPRKFGAKATGKDIQADRIVRDVKLGYEAKRDVYSAY 149
Cdd:PTZ00368  54 SCYNCGKTGHLSRECPEAPPGSGPRSCYNCGQTGHISRECPNRAKGGAARRACY 107
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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