CUE domain found in the N-terminal of TGF-beta-activated kinase 1 and MAP3K7-binding proteins TAB2, TAB3 and similar proteins; TAB2, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, or TGF-beta-activated kinase 1-binding protein 2, is an adaptor protein that regulates activation of TAK1, a MAP kinase kinase kinase (MAPKKK), through linking TAK1 to TRAF6 in the Interleukin-1 (IL-1) induced NF-kappaB activation pathway. TAB3, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, or TGF-beta-activated kinase 1-binding protein 3, is a TAB2-like TAK1-binding protein that activates NF-kappaB similar to TAB2. It activates TAK1 and regulates its association with TRAF2 and TRAF6. Moreover, TAB3 interacts with TRAF6 and TRAF2 in an IL-1- and a TNF-dependent manner, respectively. In summary, TAB2 and TAB3 function redundantly as mediators of TAK1 activation in IL-1 and TNF signal transduction. Both of them contain an N-terminal CUE domain, a coiled-coil (CC) region, a TAK1-binding domain and a C-terminal Npl4 zinc finger (NZF) ubiquitin-binding domain (UBD).

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 81880100   9 DFQVLHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVLSQES 50
Cdd:cd14362   1 DMQLFHELKQRFPEVPDAVVSQCMLQNNHNREACCAALQKES 42

Uncharacterized protein, contains DUF3084 domain [Function unknown];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100 540 QKARMERLQRELEMQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLE-EMQQLRSCNRQLQIDIDCLTKEIDLFQA 618
Cdd:COG4372  71 ARSELEQLEEELEELNEQLQAAQAELAQAQEELESLQEEAEELQEELEELQkERQDLEQQRKQLEAQIAELQSEIAEREE 150

                .
gi 81880100 619 R 619
Cdd:COG4372 151 E 151

CUE domain found in the N-terminal of TGF-beta-activated kinase 1 and MAP3K7-binding proteins TAB2, TAB3 and similar proteins; TAB2, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, or TGF-beta-activated kinase 1-binding protein 2, is an adaptor protein that regulates activation of TAK1, a MAP kinase kinase kinase (MAPKKK), through linking TAK1 to TRAF6 in the Interleukin-1 (IL-1) induced NF-kappaB activation pathway. TAB3, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, or TGF-beta-activated kinase 1-binding protein 3, is a TAB2-like TAK1-binding protein that activates NF-kappaB similar to TAB2. It activates TAK1 and regulates its association with TRAF2 and TRAF6. Moreover, TAB3 interacts with TRAF6 and TRAF2 in an IL-1- and a TNF-dependent manner, respectively. In summary, TAB2 and TAB3 function redundantly as mediators of TAK1 activation in IL-1 and TNF signal transduction. Both of them contain an N-terminal CUE domain, a coiled-coil (CC) region, a TAK1-binding domain and a C-terminal Npl4 zinc finger (NZF) ubiquitin-binding domain (UBD).

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 81880100   9 DFQVLHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVLSQES 50
Cdd:cd14362   1 DMQLFHELKQRFPEVPDAVVSQCMLQNNHNREACCAALQKES 42

CUE domain; CUE domains have been shown to bind ubiquitin. It has been suggested that CUE domains are related to pfam00627 and this has been confirmed by the structure of the domain. CUE domains also occur in two protein of the IL-1 signal transduction pathway, tollip and TAB2.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 81880100    11 QVLHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVLSQES 50
Cdd:pfam02845   3 QMLETLKEMFPDLDEEVIRAVLEASNGNVEAAINALLEGS 42

Uncharacterized protein, contains DUF3084 domain [Function unknown];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100 540 QKARMERLQRELEMQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLE-EMQQLRSCNRQLQIDIDCLTKEIDLFQA 618
Cdd:COG4372  71 ARSELEQLEEELEELNEQLQAAQAELAQAQEELESLQEEAEELQEELEELQkERQDLEQQRKQLEAQIAELQSEIAEREE 150

                .
gi 81880100 619 R 619
Cdd:COG4372 151 E 151

Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2; ROCK2 is a serine/threonine protein kinase and was the first identified target of activated RhoA. It plays a role in stress fiber and focal adhesion formation, and is prominently expressed in the brain, heart, and skeletal muscles. It is implicated in vascular and neurological disorders, such as hypertension and vasospasm of the coronary and cerebral arteries. ROCK2 is also activated by caspase-2 cleavage, resulting in thrombin-induced microparticle generation in response to cell activation. Mice deficient in ROCK2 show intrauterine growth retardation and embryonic lethality because of placental dysfunction. ROCK2 contains an N-terminal extension, a catalytic kinase domain, and a long C-terminal extension, which contains a Rho-binding HR1 domain and a pleckstrin homology (PH) domain. ROCK2 is auto-inhibited by HR1 and PH domains interacting with the catalytic domain. HR1 domains are anti-parallel coiled-coil (ACC) domains that bind small GTPases from the Rho family.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 81880100 534 QALLVHQKARMERlQRELEMQKKKLdkLKSEVNEMENNLtrRRLKRSNSISQIpSLEEMQQLRscnRQL 602
Cdd:cd11638   1 KALLQHKNTEYQR-KAEHEADRKRN--LENEVNSLKDQL--EDLKKKNQNSQI-SNEKNIQLQ---RQL 60

Leucine-rich repeats of kinetochore protein Cenp-F/LEK1; Cenp-F, a centromeric kinetochore, microtubule-binding protein consisting of two 1,600-amino acid-long coils, is essential for the full functioning of the mitotic checkpoint pathway. There are several leucine-rich repeats along the sequence of LEK1 that are considered to be zippers, though they do not appear to be binding DNA directly in this instance.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100   541 KARMERLQRELEMQKKKLDKLkseVNEMEnnltrrrlkrsNSISQIPSLE-EMQQLRSCNRQLQIDI-------DCLTKE 612
Cdd:pfam10473  23 KDKVENLERELEMSEENQELA---ILEAE-----------NSKAEVETLKaEIEEMAQNLRDLELDLvtlrsekENLTKE 88

                  ....*..
gi 81880100   613 IDLFQAR 619
Cdd:pfam10473  89 LQKKQER 95

CUE domain found in the N-terminal of TGF-beta-activated kinase 1 and MAP3K7-binding proteins TAB2, TAB3 and similar proteins; TAB2, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, or TGF-beta-activated kinase 1-binding protein 2, is an adaptor protein that regulates activation of TAK1, a MAP kinase kinase kinase (MAPKKK), through linking TAK1 to TRAF6 in the Interleukin-1 (IL-1) induced NF-kappaB activation pathway. TAB3, also called mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, or TGF-beta-activated kinase 1-binding protein 3, is a TAB2-like TAK1-binding protein that activates NF-kappaB similar to TAB2. It activates TAK1 and regulates its association with TRAF2 and TRAF6. Moreover, TAB3 interacts with TRAF6 and TRAF2 in an IL-1- and a TNF-dependent manner, respectively. In summary, TAB2 and TAB3 function redundantly as mediators of TAK1 activation in IL-1 and TNF signal transduction. Both of them contain an N-terminal CUE domain, a coiled-coil (CC) region, a TAK1-binding domain and a C-terminal Npl4 zinc finger (NZF) ubiquitin-binding domain (UBD).

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 81880100   9 DFQVLHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVLSQES 50
Cdd:cd14362   1 DMQLFHELKQRFPEVPDAVVSQCMLQNNHNREACCAALQKES 42

CUE domain; CUE domains have been shown to bind ubiquitin. It has been suggested that CUE domains are related to pfam00627 and this has been confirmed by the structure of the domain. CUE domains also occur in two protein of the IL-1 signal transduction pathway, tollip and TAB2.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 81880100    11 QVLHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVLSQES 50
Cdd:pfam02845   3 QMLETLKEMFPDLDEEVIRAVLEASNGNVEAAINALLEGS 42

Uncharacterized protein, contains DUF3084 domain [Function unknown];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100 540 QKARMERLQRELEMQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLE-EMQQLRSCNRQLQIDIDCLTKEIDLFQA 618
Cdd:COG4372  71 ARSELEQLEEELEELNEQLQAAQAELAQAQEELESLQEEAEELQEELEELQkERQDLEQQRKQLEAQIAELQSEIAEREE 150

                .
gi 81880100 619 R 619
Cdd:COG4372 151 E 151

Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2; ROCK2 is a serine/threonine protein kinase and was the first identified target of activated RhoA. It plays a role in stress fiber and focal adhesion formation, and is prominently expressed in the brain, heart, and skeletal muscles. It is implicated in vascular and neurological disorders, such as hypertension and vasospasm of the coronary and cerebral arteries. ROCK2 is also activated by caspase-2 cleavage, resulting in thrombin-induced microparticle generation in response to cell activation. Mice deficient in ROCK2 show intrauterine growth retardation and embryonic lethality because of placental dysfunction. ROCK2 contains an N-terminal extension, a catalytic kinase domain, and a long C-terminal extension, which contains a Rho-binding HR1 domain and a pleckstrin homology (PH) domain. ROCK2 is auto-inhibited by HR1 and PH domains interacting with the catalytic domain. HR1 domains are anti-parallel coiled-coil (ACC) domains that bind small GTPases from the Rho family.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 81880100 534 QALLVHQKARMERlQRELEMQKKKLdkLKSEVNEMENNLtrRRLKRSNSISQIpSLEEMQQLRscnRQL 602
Cdd:cd11638   1 KALLQHKNTEYQR-KAEHEADRKRN--LENEVNSLKDQL--EDLKKKNQNSQI-SNEKNIQLQ---RQL 60

CUE domain found in ubiquitin-binding CUE proteins; This family includes many coupling of ubiquitin conjugation to endoplasmic reticulum degradation (CUE) domain containing proteins that are characterized by an FP and a di-leucine-like sequence and bind to monoubiquitin with varying affinities. Some higher eukaryotic CUE domain proteins do not bind monoubiquitin efficiently, since they carry LP, rather than FP among CUE domains. CUE domains form three-helix bundle structures and are distantly related to the ubiquitin-associated (UBA) domains which are widely occurring ubiquitin-binding motifs found in a broad range of cellular proteins in species ranging from yeast to human. The majority of family members contain one CUE domain, but some family members from fungi harbor two CUE domains.

                        10        20        30
                ....*....|....*....|....*....|....
gi 81880100  13 LHDLRQKFPEVPEVVVSRCMLQNNNNLDACCAVL 46
Cdd:cd14279   4 LEQLQEMFPDLDEEVLEDVLEANNGDVEAAIDAL 37

Leucine-rich repeats of kinetochore protein Cenp-F/LEK1; Cenp-F, a centromeric kinetochore, microtubule-binding protein consisting of two 1,600-amino acid-long coils, is essential for the full functioning of the mitotic checkpoint pathway. There are several leucine-rich repeats along the sequence of LEK1 that are considered to be zippers, though they do not appear to be binding DNA directly in this instance.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100   541 KARMERLQRELEMQKKKLDKLkseVNEMEnnltrrrlkrsNSISQIPSLE-EMQQLRSCNRQLQIDI-------DCLTKE 612
Cdd:pfam10473  23 KDKVENLERELEMSEENQELA---ILEAE-----------NSKAEVETLKaEIEEMAQNLRDLELDLvtlrsekENLTKE 88

                  ....*..
gi 81880100   613 IDLFQAR 619
Cdd:pfam10473  89 LQKKQER 95

Breast carcinoma amplified sequence 2 (BCAS2); This family consists of several eukaryotic sequences of unknown function. The mammalian members of this family are annotated as breast carcinoma amplified sequence 2 (BCAS2) proteins. BCAS2 is a putative spliceosome associated protein.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81880100   529 DAAYTQalLVHQKARME------------------RLQRELEMQKKKLDKLKSEVNEMEnnlTRRRLKRSNSISQIPSLE 590
Cdd:pfam05700  98 DNAYAQ--LEHQRIRIEnlellqkyganawrlhnyQLEAILRRLEKELAETKEAIEEVN---RQRKNAQTAAGGELRSLE 172

                          90       100
                  ....*....|....*....|....*.
gi 81880100   591 EmQQLRSCNRQLQIDIDCLT--KEID 614
Cdd:pfam05700 173 E-KWKELVSKNLEIEAACEAleAEIL 197