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Conserved domains on  [gi|1775781343|gb|QGJ03742|]
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glycosyltransferase, partial [uncultured Brucella sp.]

Protein Classification

glycosyltransferase family protein( domain architecture ID 56)

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glycosyltransferase_GTB-type super family cl10013
glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases ...
 7-151 5.88e-08

glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. The structures of the formed glycoconjugates are extremely diverse, reflecting a wide range of biological functions. The members of this family share a common GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


The actual alignment was detected with superfamily member cd03809:

Pssm-ID: 471961 [Multi-domain]  Cd Length: 362  Bit Score: 50.44  E-value: 5.88e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343   7 HKNHAKAFQALDLYyGKLKGKIKTKIVGvssvrmdpSHRWQAKYENKAYVKSvreivagldNLKSNVEFAGEVADKEYAE 86
Cdd:cd03809   204 RKNHERLLKAFALL-KKQGGDLKLVIVG--------GKGWEDEELLDLVKKL---------GLGGRVRFLGYVSDEDLPA 265

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1775781343  87 LLASACFLWHPTLADNGTFAAVEAAYMGCPTLSNDYPQMRyisnrfEI---PMQYFNARSVKEMASAL 151
Cdd:cd03809   266 LYRGARAFVFPSLYEGFGLPVLEAMACGTPVIASNISVLP------EVagdAALYFDPLDPESIADAI 327
 
Name Accession Description Interval E-value
GT4_MtfB-like cd03809
glycosyltransferases MtfB, WbpX, and similar proteins; This family is most closely related to ...
 7-151 5.88e-08

glycosyltransferases MtfB, WbpX, and similar proteins; This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.


Pssm-ID: 340838 [Multi-domain]  Cd Length: 362  Bit Score: 50.44  E-value: 5.88e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343   7 HKNHAKAFQALDLYyGKLKGKIKTKIVGvssvrmdpSHRWQAKYENKAYVKSvreivagldNLKSNVEFAGEVADKEYAE 86
Cdd:cd03809   204 RKNHERLLKAFALL-KKQGGDLKLVIVG--------GKGWEDEELLDLVKKL---------GLGGRVRFLGYVSDEDLPA 265

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1775781343  87 LLASACFLWHPTLADNGTFAAVEAAYMGCPTLSNDYPQMRyisnrfEI---PMQYFNARSVKEMASAL 151
Cdd:cd03809   266 LYRGARAFVFPSLYEGFGLPVLEAMACGTPVIASNISVLP------EVagdAALYFDPLDPESIADAI 327
Glyco_trans_1_4 pfam13692
Glycosyl transferases group 1;
59-151 1.69e-03

Glycosyl transferases group 1;


Pssm-ID: 463957 [Multi-domain]  Cd Length: 138  Bit Score: 36.34  E-value: 1.69e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343  59 VREIVAGLDNLKSNVEFAGEVADKeyAELLASA-CFLwHPTLADNGTFAAVEAAYMGCPTLSNDYPQMRYI---SNRFEI 134
Cdd:pfam13692  44 EEELEELAAGLEDRVIFTGFVEDL--AELLAAAdVFV-LPSLYEGFGLKLLEAMAAGLPVVATDVGGIPELvdgENGLLV 120

                          90
                  ....*....|....*..
gi 1775781343 135 PmqyfnARSVKEMASAL 151
Cdd:pfam13692 121 P-----PGDPEALAEAI 132
 
Name Accession Description Interval E-value
GT4_MtfB-like cd03809
glycosyltransferases MtfB, WbpX, and similar proteins; This family is most closely related to ...
 7-151 5.88e-08

glycosyltransferases MtfB, WbpX, and similar proteins; This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.


Pssm-ID: 340838 [Multi-domain]  Cd Length: 362  Bit Score: 50.44  E-value: 5.88e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343   7 HKNHAKAFQALDLYyGKLKGKIKTKIVGvssvrmdpSHRWQAKYENKAYVKSvreivagldNLKSNVEFAGEVADKEYAE 86
Cdd:cd03809   204 RKNHERLLKAFALL-KKQGGDLKLVIVG--------GKGWEDEELLDLVKKL---------GLGGRVRFLGYVSDEDLPA 265

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1775781343  87 LLASACFLWHPTLADNGTFAAVEAAYMGCPTLSNDYPQMRyisnrfEI---PMQYFNARSVKEMASAL 151
Cdd:cd03809   266 LYRGARAFVFPSLYEGFGLPVLEAMACGTPVIASNISVLP------EVagdAALYFDPLDPESIADAI 327
GT4_PimA-like cd03801
phosphatidyl-myo-inositol mannosyltransferase; This family is most closely related to the GT4 ...
 54-123 1.34e-05

phosphatidyl-myo-inositol mannosyltransferase; This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.


Pssm-ID: 340831 [Multi-domain]  Cd Length: 366  Bit Score: 43.68  E-value: 1.34e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343  54 AYVKSVREIVAGLDNlksNVEFAGEVADKEYAELLASACFLWHPTLADNGTFAAVEAAYMGCPTLSNDYP 123
Cdd:cd03801   234 PLRAELEELELGLGD---RVRFLGFVPDEELPALYAAADVFVLPSRYEGFGLVVLEAMAAGLPVVATDVG 300
Glyco_trans_1_4 pfam13692
Glycosyl transferases group 1;
59-151 1.69e-03

Glycosyl transferases group 1;


Pssm-ID: 463957 [Multi-domain]  Cd Length: 138  Bit Score: 36.34  E-value: 1.69e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1775781343  59 VREIVAGLDNLKSNVEFAGEVADKeyAELLASA-CFLwHPTLADNGTFAAVEAAYMGCPTLSNDYPQMRYI---SNRFEI 134
Cdd:pfam13692  44 EEELEELAAGLEDRVIFTGFVEDL--AELLAAAdVFV-LPSLYEGFGLKLLEAMAAGLPVVATDVGGIPELvdgENGLLV 120

                          90
                  ....*....|....*..
gi 1775781343 135 PmqyfnARSVKEMASAL 151
Cdd:pfam13692 121 P-----PGDPEALAEAI 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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