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Conserved domains on  [gi|1958093583|gb|QQS39590|]
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GIY-YIG nuclease family protein [Candidatus Woesebacteria bacterium]

Protein Classification

GIY-YIG nuclease family protein( domain architecture ID 10006682)

GIY-YIG nuclease family protein is involved in many cellular processes, including DNA repair and recombination, transfer of mobile genetic elements, and restriction of incoming foreign DNA; similar to Clostridium perfringens UPF0213 protein CPE1444

CATH:  3.40.1440.10
Gene Ontology:  GO:0004518
PubMed:  16646971
SCOP:  3000597

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YhbQ COG2827
Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];
3-58 1.44e-13

Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];


:

Pssm-ID: 442075 [Multi-domain]  Cd Length: 82  Bit Score: 58.98  E-value: 1.44e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 1958093583  3 KRLASHKA-EQNIATKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIEGD 58
Cdd:COG2827   26 RRLAEHNSgKGAKFTRKRRPVKLVYYEEFEDRSEALKREKQIKKWSRAKKEALIEGD 82
 
Name Accession Description Interval E-value
YhbQ COG2827
Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];
3-58 1.44e-13

Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];


Pssm-ID: 442075 [Multi-domain]  Cd Length: 82  Bit Score: 58.98  E-value: 1.44e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 1958093583  3 KRLASHKA-EQNIATKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIEGD 58
Cdd:COG2827   26 RRLAEHNSgKGAKFTRKRRPVKLVYYEEFEDRSEALKREKQIKKWSRAKKEALIEGD 82
GIY-YIG_unchar_3 cd10448
GIY-YIG domain of uncharacterized hypothetical protein found in bacteria; The family includes ...
 3-56 2.01e-10

GIY-YIG domain of uncharacterized hypothetical protein found in bacteria; The family includes a group of uncharacterized bacterial proteins with a GIY-YIG domain that shows statistically significant similarity to the N-terminal catalytic domains of GIY-YIG family of intron-encoded homing endonuclease I-TevI and catalytic GIY-YIG domain of nucleotide excision repair endonuclease UvrC.


Pssm-ID: 198395  Cd Length: 87  Bit Score: 50.96  E-value: 2.01e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 1958093583  3 KRLASHKAEQNIA-TKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIE 56
Cdd:cd10448   24 RRIYEHKEGLGSGfTSKYNVTRLVYYEEFEDIEEAIAREKQLKKWRRAWKINLIE 78
PRK00329 PRK00329
GIY-YIG nuclease superfamily protein; Validated
 3-57 3.11e-06

GIY-YIG nuclease superfamily protein; Validated


Pssm-ID: 178979  Cd Length: 86  Bit Score: 40.29  E-value: 3.11e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 1958093583  3 KRLASHKAEQNI-ATKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIEG 57
Cdd:PRK00329  29 RRFAQHQSGKGAkYTRGRPPLTLVFVEPVGDRSEALRAEYRFKQLTKKQKERLVAE 84
GIY-YIG pfam01541
GIY-YIG catalytic domain; This domain called GIY-YIG is found in the amino terminal region of ...
 2-52 1.09e-03

GIY-YIG catalytic domain; This domain called GIY-YIG is found in the amino terminal region of excinuclease abc subunit c (uvrC), bacteriophage T4 endonucleases segA, segB, segC, segD and segE; it is also found in putative endonucleases encoded by group I introns of fungi and phage. The structure of I-TevI a GIY-YIG endonuclease, reveals a novel alpha/beta-fold with a central three-stranded antiparallel beta-sheet flanked by three helices. The most conserved and putative catalytic residues are located on a shallow, concave surface and include a metal coordination site.


Pssm-ID: 426314 [Multi-domain]  Cd Length: 78  Bit Score: 33.85  E-value: 1.09e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 1958093583  2 NKRLASHKAEQNIATKKFSE---LKLIYFEKYETRKEAENREIQLKKWSRAKKK 52
Cdd:pfam01541 24 ERRLNQHNAGKGAKYTRGKGvepFKLIYLEEFPTKSEALELEKYLIKLYRPNKY 77
 
Name Accession Description Interval E-value
YhbQ COG2827
Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];
3-58 1.44e-13

Predicted endonuclease, GIY-YIG superfamily [Replication, recombination and repair];


Pssm-ID: 442075 [Multi-domain]  Cd Length: 82  Bit Score: 58.98  E-value: 1.44e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 1958093583  3 KRLASHKA-EQNIATKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIEGD 58
Cdd:COG2827   26 RRLAEHNSgKGAKFTRKRRPVKLVYYEEFEDRSEALKREKQIKKWSRAKKEALIEGD 82
GIY-YIG_unchar_3 cd10448
GIY-YIG domain of uncharacterized hypothetical protein found in bacteria; The family includes ...
 3-56 2.01e-10

GIY-YIG domain of uncharacterized hypothetical protein found in bacteria; The family includes a group of uncharacterized bacterial proteins with a GIY-YIG domain that shows statistically significant similarity to the N-terminal catalytic domains of GIY-YIG family of intron-encoded homing endonuclease I-TevI and catalytic GIY-YIG domain of nucleotide excision repair endonuclease UvrC.


Pssm-ID: 198395  Cd Length: 87  Bit Score: 50.96  E-value: 2.01e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 1958093583  3 KRLASHKAEQNIA-TKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIE 56
Cdd:cd10448   24 RRIYEHKEGLGSGfTSKYNVTRLVYYEEFEDIEEAIAREKQLKKWRRAWKINLIE 78
GIY-YIG_SLX1_like cd10449
Catalytic GIY-YIG domain of yeast structure-specific endonuclease subunit SLX1 and its ...
 3-47 4.11e-07

Catalytic GIY-YIG domain of yeast structure-specific endonuclease subunit SLX1 and its homologs; Structure-specific endonuclease subunit SLX1 is a highly conserved protein from yeast to human, with an N-terminal GIY-YIG endonuclease domain and a C-terminal PHD-type zinc finger postulated to mediate protein-protein or protein-DNA interaction. SLX1 forms active heterodimeric complexes with its SLX4 partner, which has additional roles in the DNA damage response that are distinct from the function of the heterodimeric SLX1-SLX4 nuclease. In yeast, the SLX1-SLX4 complex functions as a 5' flap endonuclease that maintains ribosomal DNA copy number, where SLX1 and SLX4 are shown to be catalytic and regulatory subunits, respectively. This endonuclease introduces single-strand cuts in duplex DNA on the 3' side of junctions with single-strand DNA. In addition to 5' flap endonuclease activity, human SLX1-SLX4 complex has been identified as a Holliday junction resolvase that promotes symmetrical cleavage of static and migrating Holliday junctions. SLX1 also associates with MUS81, EME1, C20orf94, PLK1, and ERCC1. Some eukaryotic SLX1 homologs lack the zinc finger domain, but possess intrinsically unstructured extensions of unknown function. These unstructured segments might be involved in interactions with other proteins.


Pssm-ID: 198396  Cd Length: 67  Bit Score: 42.19  E-value: 4.11e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 1958093583  3 KRLASHKAEQNIATKKFSELKLIYFEKYETRKEAENREIQLKKWS 47
Cdd:cd10449   23 RRLEQHNSGKSKFTSKYRPWELVYSEAFESKSEALKREKYLKSGK 67
PRK00329 PRK00329
GIY-YIG nuclease superfamily protein; Validated
 3-57 3.11e-06

GIY-YIG nuclease superfamily protein; Validated


Pssm-ID: 178979  Cd Length: 86  Bit Score: 40.29  E-value: 3.11e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 1958093583  3 KRLASHKAEQNI-ATKKFSELKLIYFEKYETRKEAENREIQLKKWSRAKKKALIEG 57
Cdd:PRK00329  29 RRFAQHQSGKGAkYTRGRPPLTLVFVEPVGDRSEALRAEYRFKQLTKKQKERLVAE 84
GIY-YIG pfam01541
GIY-YIG catalytic domain; This domain called GIY-YIG is found in the amino terminal region of ...
 2-52 1.09e-03

GIY-YIG catalytic domain; This domain called GIY-YIG is found in the amino terminal region of excinuclease abc subunit c (uvrC), bacteriophage T4 endonucleases segA, segB, segC, segD and segE; it is also found in putative endonucleases encoded by group I introns of fungi and phage. The structure of I-TevI a GIY-YIG endonuclease, reveals a novel alpha/beta-fold with a central three-stranded antiparallel beta-sheet flanked by three helices. The most conserved and putative catalytic residues are located on a shallow, concave surface and include a metal coordination site.


Pssm-ID: 426314 [Multi-domain]  Cd Length: 78  Bit Score: 33.85  E-value: 1.09e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 1958093583  2 NKRLASHKAEQNIATKKFSE---LKLIYFEKYETRKEAENREIQLKKWSRAKKK 52
Cdd:pfam01541 24 ERRLNQHNAGKGAKYTRGKGvepFKLIYLEEFPTKSEALELEKYLIKLYRPNKY 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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