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Conserved domains on  [gi|2070269241|gb|QXR34213|]
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MobC family plasmid mobilization relaxosome protein (plasmid) [Enterobacter hormaechei]

Protein Classification

MobC family plasmid mobilization relaxosome protein( domain architecture ID 10529718)

MobC family plasmid mobilization relaxosome protein such as Escherichia coli mobilization protein MbeC, which is essential to promote the transfer of ColE1 plasmid during conjugation, and probably functions by inducing DNA bending, helping the MbeA relaxase to melt the DNA around the nic site and cleave the phosphodiester bond

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MobC pfam05713
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ...
 50-94 2.49e-11

Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA.


:

Pssm-ID: 428600  Cd Length: 45  Bit Score: 53.64  E-value: 2.49e-11
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2070269241  50 LRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERL 94
Cdd:pfam05713   1 LRQLRGIGNNINQIARLANSGYGSFAVLAELVSALAEINQELDQL 45
 
Name Accession Description Interval E-value
MobC pfam05713
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ...
 50-94 2.49e-11

Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA.


Pssm-ID: 428600  Cd Length: 45  Bit Score: 53.64  E-value: 2.49e-11
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2070269241  50 LRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERL 94
Cdd:pfam05713   1 LRQLRGIGNNINQIARLANSGYGSFAVLAELVSALAEINQELDQL 45
 
Name Accession Description Interval E-value
MobC pfam05713
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ...
 50-94 2.49e-11

Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA.


Pssm-ID: 428600  Cd Length: 45  Bit Score: 53.64  E-value: 2.49e-11
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2070269241  50 LRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERL 94
Cdd:pfam05713   1 LRQLRGIGNNINQIARLANSGYGSFAVLAELVSALAEINQELDQL 45
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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