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Conserved domains on  [gi|1519845290|gb|RPH95865|]
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MAG: succinylglutamate desuccinylase [Lysobacterales bacterium]

Protein Classification

M14_ASTE_ASPA_like and Biotinyl_lipoyl_domains domain-containing protein( domain architecture ID 13031230)

M14_ASTE_ASPA_like and Biotinyl_lipoyl_domains domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 80-266 1.54e-96

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


:

Pssm-ID: 349484  Cd Length: 187  Bit Score: 284.90  E-value: 1.54e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTSPADGKNLNRVYPGRADGSLSERIADVI 159
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 160 TRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAASRGMALAFGLDHIVIDEGRAQPPEASIYTDQTALSRGIPA 239
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETGNAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIPA 160

                         170       180
                  ....*....|....*....|....*..
gi 1519845290 240 ITTETGQLGSNDEHWVGLAVFGAENLM 266
Cdd:cd18174   161 ILVEAGGLGSRDEEDVARHVEGVLNVL 187
Biotinyl_lipoyl_domains super family cl11404
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 290-353 6.13e-03

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


The actual alignment was detected with superfamily member cd06850:

Pssm-ID: 448245 [Multi-domain]  Cd Length: 67  Bit Score: 35.08  E-value: 6.13e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1519845290 290 VIRSPITGIF-QPAVRDGYAVAEGGLLG--------HlldefgepagEVRAPFAGVVNYVLGTP--PVSQGEPLA 353
Cdd:cd06850     1 EVTAPMPGTVvKVLVKEGDKVEAGQPLAvleamkmeN----------EVTAPVAGVVKEILVKEgdQVEAGQLLV 65
 
Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 80-266 1.54e-96

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 284.90  E-value: 1.54e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTSPADGKNLNRVYPGRADGSLSERIADVI 159
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 160 TRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAASRGMALAFGLDHIVIDEGRAQPPEASIYTDQTALSRGIPA 239
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETGNAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIPA 160

                         170       180
                  ....*....|....*....|....*..
gi 1519845290 240 ITTETGQLGSNDEHWVGLAVFGAENLM 266
Cdd:cd18174   161 ILVEAGGLGSRDEEDVARHVEGVLNVL 187
COG3608 COG3608
Predicted deacylase [General function prediction only];
65-355 5.76e-81

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 248.99  E-value: 5.76e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  65 RIPVTLVHGARPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTvYTSPADGKNLNRVYP 144
Cdd:COG3608    14 SLPVTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRELDPGELRGTVILVPVANPPGFLQGS-RYLPIDGRDLNRSFP 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 145 GRADGSLSERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPvtgqaELDAASRGMALAFGLDHIVIDegraqPPEA 224
Cdd:COG3608    93 GDADGSLAERIAHALFEEILPDADYVIDLHSGGIARDNLPHVRAG-----PGDEELRALARAFGAPVILDS-----PEGG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 225 SIYTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGMLPGAA--RANDNVVWLRGYEVIRSPITGIFQPA 302
Cdd:COG3608   163 DGSLREAAAEAGIPALTLELGGGGRFDEESIEAGVRGILNVLRHLGMLDGEAppPPLAPPVLARGSEWVRAPAGGLFEPL 242

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1519845290 303 VRDGYAVAEGGLLGHLLDEFGEPAGEVRAPFAGVVNYVLGTPPVSQGEPLAMV 355
Cdd:COG3608   243 VELGDRVKKGDVLGRITDPFGEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHI 295
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 76-355 8.21e-34

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 126.70  E-value: 8.21e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  76 PGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYtspaDGKNLNRVYPGRADGSLS--- 152
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRY----IPRDLNRSFPGRALGASSdep 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 153 ------ERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAAsrgMALAFGLDHIVidegRAQPPEASI 226
Cdd:pfam04952  77 yratraERLADLFFPALLPRADIVLDLHTGTRGMGHLLFALAPIRDDPLHLLA---LLRAFGAPAVL----KLHSKPSAG 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 227 YTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGMLPGAARANDNVVWLR------GYEVIRSPITGIFQ 300
Cdd:pfam04952 150 FSAFSAEELGAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPPKLYRvlreidRPRDIRAELAGLVE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1519845290 301 PAVRDGYAVAEGGLL--GHLLDEFGEPAGEVRAPFAGVVNYVLGTPPVSQGEPLAMV 355
Cdd:pfam04952 230 FALNLGDDVDAGPLLpgGPLFAPFGGEETEYRAPEDGYPVFPNEAAYVGKGAALALV 286
biotinyl_domain cd06850
The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all ...
 290-353 6.13e-03

The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all biotin-dependent enzymes, such as acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, geranyl-CoA carboxylase, oxaloacetate decarboxylase, methylmalonyl-CoA decarboxylase, transcarboxylase and urea amidolyase. This domain functions in transferring CO2 from one subsite to another, allowing carboxylation, decarboxylation, or transcarboxylation. During this process, biotin is covalently attached to a specific lysine.


Pssm-ID: 133459 [Multi-domain]  Cd Length: 67  Bit Score: 35.08  E-value: 6.13e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1519845290 290 VIRSPITGIF-QPAVRDGYAVAEGGLLG--------HlldefgepagEVRAPFAGVVNYVLGTP--PVSQGEPLA 353
Cdd:cd06850     1 EVTAPMPGTVvKVLVKEGDKVEAGQPLAvleamkmeN----------EVTAPVAGVVKEILVKEgdQVEAGQLLV 65
 
Name Accession Description Interval E-value
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 80-266 1.54e-96

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 284.90  E-value: 1.54e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTSPADGKNLNRVYPGRADGSLSERIADVI 159
Cdd:cd18174     1 LLVTAGVHGYEYASIEALQRLIKELDPAKLSGTVIVVPIANIPAFEGRSIYVNPLDGKNLNRSFPGDPDGTPTERLAHWL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 160 TRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAASRGMALAFGLDHIVIDEGRAQPPEASIYTDQTALSRGIPA 239
Cdd:cd18174    81 TTNVIARADYYIDLHGGDLNEDLRPFVYYYETGNAALDAASREMAEAFGLDHIVFYKARLKASRGSLYTQAAALLRGIPA 160

                         170       180
                  ....*....|....*....|....*..
gi 1519845290 240 ITTETGQLGSNDEHWVGLAVFGAENLM 266
Cdd:cd18174   161 ILVEAGGLGSRDEEDVARHVEGVLNVL 187
M14_ASTE_ASPA-like cd06254
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 67-269 1.60e-88

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349472  Cd Length: 198  Bit Score: 264.83  E-value: 1.60e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  67 PVTLVHGARPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTSPADGKNLNRVYPGR 146
Cdd:cd06254     1 PVTLINGAKPGPTLLITAGIHGGEYPGILAAIRLARELDPADVKGTLIIVHIANVSGFEARTPFVVPEDGKNLNRVFPGD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 147 ADGSLSERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAASRGMALAFGLDHIVIDEgraqpPEASI 226
Cdd:cd06254    81 PDGTLTERIAYFLTREIISRADFLIDLHGGDANEALTPFVYYPGGASEEVNDISRAAAQALGLPYIVISS-----SEKGT 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1519845290 227 YTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHL 269
Cdd:cd06254   156 GYYSYAALRGIPSILVERGGLGTCDEEDVQAHKDGIKNLLRHL 198
COG3608 COG3608
Predicted deacylase [General function prediction only];
65-355 5.76e-81

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 248.99  E-value: 5.76e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  65 RIPVTLVHGARPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTvYTSPADGKNLNRVYP 144
Cdd:COG3608    14 SLPVTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRELDPGELRGTVILVPVANPPGFLQGS-RYLPIDGRDLNRSFP 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 145 GRADGSLSERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPvtgqaELDAASRGMALAFGLDHIVIDegraqPPEA 224
Cdd:COG3608    93 GDADGSLAERIAHALFEEILPDADYVIDLHSGGIARDNLPHVRAG-----PGDEELRALARAFGAPVILDS-----PEGG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 225 SIYTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGMLPGAA--RANDNVVWLRGYEVIRSPITGIFQPA 302
Cdd:COG3608   163 DGSLREAAAEAGIPALTLELGGGGRFDEESIEAGVRGILNVLRHLGMLDGEAppPPLAPPVLARGSEWVRAPAGGLFEPL 242

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1519845290 303 VRDGYAVAEGGLLGHLLDEFGEPAGEVRAPFAGVVNYVLGTPPVSQGEPLAMV 355
Cdd:COG3608   243 VELGDRVKKGDVLGRITDPFGEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHI 295
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 66-272 7.29e-47

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 158.09  E-value: 7.29e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  66 IPVTLVHGARPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTsPADGKNLNRVYPG 145
Cdd:cd06251     1 VPVLVARGAKPGPTLLLTAAIHGDELNGIEVIQRLLEDLDPSKLRGTLIAIPVVNPLGFENNSRYL-PDDGRDLNRSFPG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 146 RADGSLSERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPVTgqaelDAASRGMALAFGLDHIVIDEGRAQppeas 225
Cdd:cd06251    80 SEKGSLASRLAHLLWNEIVKKADYVIDLHTASTGRTNLPYVRADLR-----DPESRRMAEAFGAPVIVDDPGEDG----- 149

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1519845290 226 iYTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGML 272
Cdd:cd06251   150 -SLRGAAVELGIPAITVELGEALRFDEDIIRRGVEGVLNVLRHLGML 195
M14_ASTE_ASPA-like cd06255
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 65-272 3.21e-44

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349473  Cd Length: 223  Bit Score: 152.09  E-value: 3.21e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  65 RIPVTLVHGARPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTvYTSPADGKNLNRVYP 144
Cdd:cd06255    11 TIPVIVVRGAKPGPCLWINGAVHGDELNGPLAALELFRELDPAQLSGTLVATPIANPLAFQGRQ-KFSPQDGEDLDQSFP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 145 GRADGSLSERIADVITRQVIERADYLVDLH-GGDGNEALRPYVY-LPVTGQAELDAASRGMALAFGLD-HIVID---EGR 218
Cdd:cd06255    90 GDPDGLITERMAHALFSEVKEVADYLIDFHtGGTPFDANPYTVYkLFPESGPVEEKRLLRLARAFGVHaNCRVDvsgAGG 169

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1519845290 219 AQPPEASIYTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGML 272
Cdd:cd06255   170 ELPGNTAGALDYQCMAQGIPAFMVELGGGGRAEEEAVRFAARGLRNLLRYLGML 223
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 80-265 5.95e-43

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 147.07  E-value: 5.95e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTsPADGKNLNRVYPGRADGSLSERIADVI 159
Cdd:cd06230     1 LLILAGVHGDEYEGVEAIRRLLAELDPSELKGTVVLVPVANPPAFEAGTRYT-PLDGLDLNRIFPGDPDGSPTERLAHEL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 160 TRQVIERADYLVDLHGGdGNEALRPYVYLPVTGQAelDAASRGMALAFGLDHIVidegRAQPPEASIyTDQTALSRGIPA 239
Cdd:cd06230    80 TELILKHADALIDLHSG-GTGRLVPYAILDYDSDA--REKSRELARAFGGTPVI----WGGDPPGGT-PVAAARSAGIPA 151

                         170       180
                  ....*....|....*....|....*.
gi 1519845290 240 ITTETGQLGSNDEHWVGLAVFGAENL 265
Cdd:cd06230   152 ITVELGGGGRLRAERLERYLRGIRNV 177
M14_ASTE_ASPA-like cd06252
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 65-272 3.71e-38

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349470  Cd Length: 224  Bit Score: 136.16  E-value: 3.71e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  65 RIPVTLVHGaRPGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTvYTSPADGKNLNRVYP 144
Cdd:cd06252    23 PIPITVINN-GSGPTVLLTGGNHGDEYEGPIALRRLARDLDPEDVRGRLIIVPALNLPAVRAGT-RTSPLDGGNLNRAFP 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 145 GRADGSLSERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAASRGMALAFGLDHIVIDEGRAQPPea 224
Cdd:cd06252   101 GDADGTPTERIAHFLETVLLPRADAVIDLHSGGSSLDFVPCAAVHLLPDPAQRARSLALAEAFGAPLSVVVDNVDAPG-- 178

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1519845290 225 siyTDQTALSR-GIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGML 272
Cdd:cd06252   179 ---TLDSAAERaGKIFVSTELGGGGTVTPAALRIAERGVLNVLIHLGVL 224
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 76-355 8.21e-34

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 126.70  E-value: 8.21e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  76 PGPALALIAGTHGYEYPAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYtspaDGKNLNRVYPGRADGSLS--- 152
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRY----IPRDLNRSFPGRALGASSdep 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 153 ------ERIADVITRQVIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAAsrgMALAFGLDHIVidegRAQPPEASI 226
Cdd:pfam04952  77 yratraERLADLFFPALLPRADIVLDLHTGTRGMGHLLFALAPIRDDPLHLLA---LLRAFGAPAVL----KLHSKPSAG 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 227 YTDQTALSRGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLGMLPGAARANDNVVWLR------GYEVIRSPITGIFQ 300
Cdd:pfam04952 150 FSAFSAEELGAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPPKLYRvlreidRPRDIRAELAGLVE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1519845290 301 PAVRDGYAVAEGGLL--GHLLDEFGEPAGEVRAPFAGVVNYVLGTPPVSQGEPLAMV 355
Cdd:pfam04952 230 FALNLGDDVDAGPLLpgGPLFAPFGGEETEYRAPEDGYPVFPNEAAYVGKGAALALV 286
M14_ASTE_ASPA-like cd06253
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 73-270 1.57e-16

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349471  Cd Length: 211  Bit Score: 77.25  E-value: 1.57e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  73 GARPGPALALIAGTHGYEYPAI-TALQ---RLRGR-VAPESLAGTLILVHIANPPSFYGRTVYtSPADGKNLNRVYPGRA 147
Cdd:cd06253    18 GGNAEPRIAIVAGIHGDELNGLyVCSRlirFLKELeEGGYKLKGKVLVIPAVNPLGINSGTRF-WPFDNLDMNRMFPGYN 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 148 DGSLSERIADVITRQvIERADYLVDLHGGDGNEALRPYVYLPVTGQAELDAasrgMALAFGLDHIVIdegraqpPEASIY 227
Cdd:cd06253    97 KGETTERIAAALFED-LKGADYGIDLHSSNDFLREIPQVRVIESGAQDLLP----LAKFLGLDVVWV-------HPASTV 164

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1519845290 228 TDQT---ALS-RGIPAITTETGQLGSNDEHWVGLAVFGAENLMRHLG 270
Cdd:cd06253   165 DTGTlayNWNeWGTKALVLEMGVGMRIDKEYCEQLFEGILRFLLKMG 211
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 80-174 1.67e-06

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 47.44  E-value: 1.67e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYPAITALQRLR-GRVAPESLAGTLILVhIANPPSFYGRTVYTSpadgKNLNRVYPGRADGSLSE-RIAD 157
Cdd:cd18430     1 LAVLGAVHGNETCGTRAVERLLaELPSGALQKGPVTLV-PANERAYAEGVRFCE----EDLNRVFPGDPDPDTYErRLAN 75

                          90
                  ....*....|....*..
gi 1519845290 158 VItRQVIERADYLVDLH 174
Cdd:cd18430    76 RL-CPELEGHDVVLDLH 91
Peptidase_M14_like cd00596
M14 family of metallocarboxypeptidases and related proteins; The M14 family of ...
 80-245 1.61e-04

M14 family of metallocarboxypeptidases and related proteins; The M14 family of metallocarboxypeptidases (MCPs), also known as funnelins, are zinc-binding carboxypeptidases (CPs) which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Two major subfamilies of the M14 family, defined based on sequence and structural homology, are the A/B and N/E subfamilies. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide, followed by an N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity; Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. The A forms have slightly different specificities, with Carboxypeptidase A1 (CPA1) preferring aliphatic and small aromatic residues, and CPA2 preferring the bulky aromatic side chains. Enzymes belonging to the N/E subfamily enzymes are not produced as inactive precursors and instead rely on their substrate specificity and subcellular compartmentalization to prevent inappropriate cleavage. They contain an extra C-terminal transthyretin-like domain, thought to be involved in folding or formation of oligomers. MCPs can also be classified based on their involvement in specific physiological processes; the pancreatic MCPs participate only in alimentary digestion and include carboxypeptidase A and B (A/B subfamily), while others, namely regulatory MCPs or the N/E subfamily, are involved in more selective reactions, mainly in non-digestive tissues and fluids, acting on blood coagulation/fibrinolysis, inflammation and local anaphylaxis, pro-hormone and neuropeptide processing, cellular response and others. Another MCP subfamily, is that of succinylglutamate desuccinylase /aspartoacylase, which hydrolyzes N-acetyl-L-aspartate (NAA), and deficiency in which is the established cause of Canavan disease. Another subfamily (referred to as subfamily C) includes an exceptional type of activity in the MCP family, that of dipeptidyl-peptidase activity of gamma-glutamyl-(L)-meso-diaminopimelate peptidase I which is involved in bacterial cell wall metabolism.


Pssm-ID: 349427 [Multi-domain]  Cd Length: 216  Bit Score: 42.45  E-value: 1.61e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  80 LALIAGTHGYEYP----AITALQRLRGRVAPESLAG-----TLILVHIANPPSFYGRTVYTSPAD--GKNLNRVYP---- 144
Cdd:cd00596     1 ILITGGIHGNEVIgvelALALIEYLLENYGNDPLKRlldnvELWIVPLVNPDGFARVIDSGGRKNanGVDLNRNFPynwg 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 145 -----------GRADGSLSER----IADVITRQvieRADYLVDLHGGdGNEALRPYVY--LPVTGQAELDAASRGMALAF 207
Cdd:cd00596    81 kdgtsgpssptYRGPAPFSEPetqaLRDLAKSH---RFDLAVSYHSS-SEAILYPYGYtnEPPPDFSEFQELAAGLARAL 156

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1519845290 208 GLDHIVIDEGRAQPPEASIYTDQTALSRGIPAITTETG 245
Cdd:cd00596   157 GAGEYGYGYSYTWYSTTGTADDWLYGELGILAFTVELG 194
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 76-254 4.02e-03

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 38.10  E-value: 4.02e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290  76 PGPALALIAGTHGYEY-PAITALQRLRGRVAPESLAGTLILVHIANPPSFYGRTVYTSPADGKNLNRVY-PGRADG---S 150
Cdd:cd06910    23 PGPHVMINALTHGNEIcGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTASRFVDEDLNRVWgPELLDGpeqS 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519845290 151 LSERIADVItRQVIERADYLVDLHggDGNEALRPyvyLPVTGQAEldaasRGMALAFGLD---HIVIDEGRAQPPEASIY 227
Cdd:cd06910   103 IELRRAREL-RPVVDTVDYLLDIH--SMQEKVPP---LALAGDKE-----KGRALARRVGspaHLLIDAGHAEGLRLRDY 171

                         170       180
                  ....*....|....*....|....*..
gi 1519845290 228 TDQTALSRGIPAITTETGQlgsndeHW 254
Cdd:cd06910   172 AGFGDPSSPKNALLVECGQ------HW 192
biotinyl_domain cd06850
The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all ...
 290-353 6.13e-03

The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all biotin-dependent enzymes, such as acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, geranyl-CoA carboxylase, oxaloacetate decarboxylase, methylmalonyl-CoA decarboxylase, transcarboxylase and urea amidolyase. This domain functions in transferring CO2 from one subsite to another, allowing carboxylation, decarboxylation, or transcarboxylation. During this process, biotin is covalently attached to a specific lysine.


Pssm-ID: 133459 [Multi-domain]  Cd Length: 67  Bit Score: 35.08  E-value: 6.13e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1519845290 290 VIRSPITGIF-QPAVRDGYAVAEGGLLG--------HlldefgepagEVRAPFAGVVNYVLGTP--PVSQGEPLA 353
Cdd:cd06850     1 EVTAPMPGTVvKVLVKEGDKVEAGQPLAvleamkmeN----------EVTAPVAGVVKEILVKEgdQVEAGQLLV 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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