NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1079661938|emb|SCW84249|]
View 

Succinylglutamate desuccinylase [Ralstonia sp. UNCCL144]

Protein Classification

M14 family metallopeptidase( domain architecture ID 10161028)

M14 family metallopeptidase is a zinc-binding carboxypeptidase which hydrolyzes a single, C-terminal amino acid from a polypeptide chain, and has a recognition site for the free C-terminal carboxyl group

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 22-229 1.31e-139

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


:

Pssm-ID: 349481  Cd Length: 208  Bit Score: 393.64  E-value: 1.31e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  22 DIRRWKTGTHGIDYVHTFDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 101
Cdd:cd06910     1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 102 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 181
Cdd:cd06910    81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1079661938 182 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 229
Cdd:cd06910   161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
 
Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 22-229 1.31e-139

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 393.64  E-value: 1.31e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  22 DIRRWKTGTHGIDYVHTFDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 101
Cdd:cd06910     1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 102 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 181
Cdd:cd06910    81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1079661938 182 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 229
Cdd:cd06910   161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
COG3608 COG3608
Predicted deacylase [General function prediction only];
36-322 3.77e-36

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 131.89  E-value: 3.77e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  36 VHTFDSGVPGPHVMINALTHGNELCGAIAVDALLAAgVRPA--RGRLTL-SFANVEAYERFdindpdaTRFI---DEDLN 109
Cdd:COG3608    17 VTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRE-LDPGelRGTVILvPVANPPGFLQG-------SRYLpidGRDLN 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 110 RVWSADKlDGpgnSLELRRAREL-RPVIDTVDYLLDIHSMHEKAE--PLMLTGP-HAKGVALGRLVGAPshLMIDAGHAA 185
Cdd:COG3608    89 RSFPGDA-DG---SLAERIAHALfEEILPDADYVIDLHSGGIARDnlPHVRAGPgDEELRALARAFGAP--VILDSPEGG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 186 GRRLRDYGgfgdAASPKNALLIECGQH--FERVSRDVALDACARFLVALGTVDASAVSewlgmAPALEVRCIRVTDPVVA 263
Cdd:COG3608   163 DGSLREAA----AEAGIPALTLELGGGgrFDEESIEAGVRGILNVLRHLGMLDGEAPP-----PPLAPPVLARGSEWVRA 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1079661938 264 KSTA-FRFaddYRGMETIARAGTVIAT------DGALQIVTPYDNCVLLQPSLRHLAPGVTVVRLG 322
Cdd:COG3608   234 PAGGlFEP---LVELGDRVKKGDVLGRitdpfgEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 44-147 6.90e-10

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 58.90  E-value: 6.90e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  44 PGPHVMINALTHGNELCGAIAVDAL---LAAGVRPARgRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP 120
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLlrqLDPGDIAGE-RTLVPLANPPAFRA-------GSRYIPRDLNRSFPGRALGAS 72

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1079661938 121 G-NSLELRRAREL-----RPVIDTVDYLLDIHS 147
Cdd:pfam04952  73 SdEPYRATRAERLadlffPALLPRADIVLDLHT 105
PRK02259 PRK02259
aspartoacylase; Provisional
 48-171 7.28e-10

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 58.73  E-value: 7.28e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPGNS-L 124
Cdd:PRK02259    5 VAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTviGNPEAIEA-------GRRYIDRDLNRSFRLDLLQNPDLSgY 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1079661938 125 ELRRARELRPVI-----DTVDYLLDIHS----MhekAEPLMLTGPHAKGVALGRLV 171
Cdd:PRK02259   78 EQLRAKELVQQLgpkgnSPCDFIIDLHSttanM---GLSLILYGRRPFDLALAAYL 130
 
Name Accession Description Interval E-value
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 22-229 1.31e-139

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 393.64  E-value: 1.31e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  22 DIRRWKTGTHGIDYVHTFDSGVPGPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERFDINDPDAT 101
Cdd:cd06910     1 DISPYKTGNTGIDYVHRFDSGQPGPHVMINALTHGNEICGAIALDWLLKNGVRPLRGRLTFCFANVEAYERFDPARPTAS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 102 RFIDEDLNRVWSADKLDGPGNSLELRRARELRPVIDTVDYLLDIHSMHEKAEPLMLTGPHAKGVALGRLVGAPSHLMIDA 181
Cdd:cd06910    81 RFVDEDLNRVWGPELLDGPEQSIELRRARELRPVVDTVDYLLDIHSMQEKVPPLALAGDKEKGRALARRVGSPAHLLIDA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1079661938 182 GHAAGRRLRDYGGFGDAASPKNALLIECGQHFERVSRDVALDACARFL 229
Cdd:cd06910   161 GHAEGLRLRDYAGFGDPSSPKNALLVECGQHWERSAVVVALDITLRFL 208
COG3608 COG3608
Predicted deacylase [General function prediction only];
36-322 3.77e-36

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 131.89  E-value: 3.77e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  36 VHTFDSGVPGPHVMINALTHGNELCGAIAVDALLAAgVRPA--RGRLTL-SFANVEAYERFdindpdaTRFI---DEDLN 109
Cdd:COG3608    17 VTVFRGAGPGPTLLITAGIHGDELNGIEALRRLLRE-LDPGelRGTVILvPVANPPGFLQG-------SRYLpidGRDLN 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 110 RVWSADKlDGpgnSLELRRAREL-RPVIDTVDYLLDIHSMHEKAE--PLMLTGP-HAKGVALGRLVGAPshLMIDAGHAA 185
Cdd:COG3608    89 RSFPGDA-DG---SLAERIAHALfEEILPDADYVIDLHSGGIARDnlPHVRAGPgDEELRALARAFGAP--VILDSPEGG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 186 GRRLRDYGgfgdAASPKNALLIECGQH--FERVSRDVALDACARFLVALGTVDASAVSewlgmAPALEVRCIRVTDPVVA 263
Cdd:COG3608   163 DGSLREAA----AEAGIPALTLELGGGgrFDEESIEAGVRGILNVLRHLGMLDGEAPP-----PPLAPPVLARGSEWVRA 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1079661938 264 KSTA-FRFaddYRGMETIARAGTVIAT------DGALQIVTPYDNCVLLQPSLRHLAPGVTVVRLG 322
Cdd:COG3608   234 PAGGlFEP---LVELGDRVKKGDVLGRitdpfgEEVEEVRAPVDGIVIGRRTNPLVNPGDALFHIA 296
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 48-228 2.26e-18

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 80.95  E-value: 2.26e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNELCGAIAVDALLAAGVRPA--RGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKldgPGNSLE 125
Cdd:cd18430     1 LAVLGAVHGNETCGTRAVERLLAELPSGAlqKGPVTLVPANERAYAE-------GVRFCEEDLNRVFPGDP---DPDTYE 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 126 LRRARELRPVIDTVDYLLDIHSMHEKAEPL-MLTGPHAKGVALGRLVGAPSHL-MIDaghaaGRRLRDYGGFGdaASPKN 203
Cdd:cd18430    71 RRLANRLCPELEGHDVVLDLHSTHSGGQPFaILDYGDKASRRLARSVGIPKGWrVVY-----GRDLGYAHGGG--KTEVT 143

                         170       180
                  ....*....|....*....|....*
gi 1079661938 204 ALLIECGQHFERVSRDVALDACARF 228
Cdd:cd18430   144 GVTVECGYHDSEEAAEVAYRAILNT 168
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 48-147 1.48e-15

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349480  Cd Length: 190  Bit Score: 73.78  E-value: 1.48e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP--GNS 123
Cdd:cd06909     3 VAIVGGTHGNELTGVYLVKHWLKNPELIERKSFEVHPllANPRAVEQ-------CRRYIDTDLNRCFSLENLSSApsSLP 75

                          90       100
                  ....*....|....*....|....*....
gi 1079661938 124 LELRRAREL----RPVIDT-VDYLLDIHS 147
Cdd:cd06909    76 YEVRRAREInqilGPKGNPaCDFIIDLHN 104
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
45-147 8.02e-15

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 73.73  E-value: 8.02e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  45 GPHVMINALTHGNELCGAIAVDAL---LAAGVRPARGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPG 121
Cdd:COG2988    24 IKAVVISGGIHGNETAPIELLDKLlqdLLLGERPLSFRLLLILGNPAAMRA-------GRRYLDEDLNRLFGGRHLQNPE 96

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1079661938 122 NsLELRRARELRPVID-------TVDYLLDIHS 147
Cdd:COG2988    97 S-YEAARAKELEQAVGpffaaggRVRLHIDLHT 128
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 48-226 1.07e-14

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 71.19  E-value: 1.07e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNELCGAIAVDALLAAGVRPA-RGRLTL-SFANVEAYERfdindpdATRFIDE---DLNRVWSADkldgPGN 122
Cdd:cd06230     1 LLILAGVHGDEYEGVEAIRRLLAELDPSElKGTVVLvPVANPPAFEA-------GTRYTPLdglDLNRIFPGD----PDG 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 123 SLELRRARELRP-VIDTVDYLLDIHSMHEKAEP--LMLTGPHAKG---VALGRLVGAPSHLMIDaGHAAGrrlRDYGGFG 196
Cdd:cd06230    70 SPTERLAHELTElILKHADALIDLHSGGTGRLVpyAILDYDSDAReksRELARAFGGTPVIWGG-DPPGG---TPVAAAR 145

                         170       180       190
                  ....*....|....*....|....*....|
gi 1079661938 197 DAASPknALLIECGQHFERVSRDVALDACA 226
Cdd:cd06230   146 SAGIP--AITVELGGGGRLRAERLERYLRG 173
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 44-147 6.90e-10

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 58.90  E-value: 6.90e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  44 PGPHVMINALTHGNELCGAIAVDAL---LAAGVRPARgRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGP 120
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLlrqLDPGDIAGE-RTLVPLANPPAFRA-------GSRYIPRDLNRSFPGRALGAS 72

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1079661938 121 G-NSLELRRAREL-----RPVIDTVDYLLDIHS 147
Cdd:pfam04952  73 SdEPYRATRAERLadlffPALLPRADIVLDLHT 105
PRK02259 PRK02259
aspartoacylase; Provisional
 48-171 7.28e-10

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 58.73  E-value: 7.28e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSF--ANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPGNS-L 124
Cdd:PRK02259    5 VAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTviGNPEAIEA-------GRRYIDRDLNRSFRLDLLQNPDLSgY 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1079661938 125 ELRRARELRPVI-----DTVDYLLDIHS----MhekAEPLMLTGPHAKGVALGRLV 171
Cdd:PRK02259   78 EQLRAKELVQQLgpkgnSPCDFIIDLHSttanM---GLSLILYGRRPFDLALAAYL 130
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 48-151 1.24e-07

succinylglutamate desuccinylase; Provisional


Pssm-ID: 235408  Cd Length: 329  Bit Score: 52.49  E-value: 1.24e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  48 VMINALTHGNE-----LCGAIAVDalLAAGVRPARGRLTLSFANVEAyerfdINDpdATRFIDEDLNRVWSADKLDGPGn 122
Cdd:PRK05324   50 LVLSAGIHGNEtapieLLDQLVRD--LLAGELPLRARLLVILGNPPA-----MRA--GKRYLDEDLNRLFGGRHQQFPG- 119

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1079661938 123 SLELRRARELRPVIDT--------VDYLLDIH-----SMHEK 151
Cdd:PRK05324  120 SDEARRAAELEQAVEDffaagaerVRWHYDLHtairgSKHEQ 161
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 44-233 2.38e-06

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 47.53  E-value: 2.38e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  44 PGPHVMINALTHGNELCGaIAVDALLAAGVRPA--RGRLT-LSFANVEAYERFDINDPDATRfideDLNRVWsadkLDGP 120
Cdd:cd06251    11 PGPTLLLTAAIHGDELNG-IEVIQRLLEDLDPSklRGTLIaIPVVNPLGFENNSRYLPDDGR----DLNRSF----PGSE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 121 GNSLELRRARELRP-VIDTVDYLLDIH--SMHEKAEPLML-TGPHAKGVALGRLVGAPshlMIDagHAAGRRlrdyGGFG 196
Cdd:cd06251    82 KGSLASRLAHLLWNeIVKKADYVIDLHtaSTGRTNLPYVRaDLRDPESRRMAEAFGAP---VIV--DDPGED----GSLR 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1079661938 197 DAASPKN--ALLIECGQH--FERVSRDVALDACARFLVALG 233
Cdd:cd06251   153 GAAVELGipAITVELGEAlrFDEDIIRRGVEGVLNVLRHLG 193
M14_ASTE cd03855
Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 ...
 55-151 2.53e-05

Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 Succinylglutamate desuccinylase (ASTE, also known as N-succinyl-L-glutamate amidohydrolase, N2-succinylglutamate desuccinylase, and SGDS; EC 3.5.1.96) belongs to the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily of the M14 family of metallocarboxypeptidases. This group includes succinylglutamate desuccinylase that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. It hydrolyzes N-succinyl-L-glutamate to succinate and L-glutamate.


Pssm-ID: 349428  Cd Length: 239  Bit Score: 44.89  E-value: 2.53e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  55 HGNE-----LCGAIAVDalLAAGVRPARGRLTLSFANVEAYERfdindpdATRFIDEDLNRVWSADKLDGPgNSLELRRA 129
Cdd:cd03855    53 HGNEtapieILDQLIND--LIRGELALAHRLLFIFGNPPAIRQ-------GKRFIEENLNRLFSGRHSKLP-PSYETARA 122

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1079661938 130 RELRPVID--------TVDYLLDIH-----SMHEK 151
Cdd:cd03855   123 AELEQAVAdffakasgEVRWHLDLHtairgSKHEQ 157
M14_ASTE_ASPA-like cd06254
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 43-154 5.06e-05

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349472  Cd Length: 198  Bit Score: 43.34  E-value: 5.06e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  43 VPGPHVMINALTHGNELCGAIAVdALLAAGVRPAR--GRLTL-SFANVEAYE--RFDINdpdatrFIDED-LNRVWSADK 116
Cdd:cd06254     9 KPGPTLLITAGIHGGEYPGILAA-IRLARELDPADvkGTLIIvHIANVSGFEarTPFVV------PEDGKnLNRVFPGDP 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1079661938 117 LDGPGNSLELRRARElrpVIDTVDYLLDIHS--MHEKAEP 154
Cdd:cd06254    82 DGTLTERIAYFLTRE---IISRADFLIDLHGgdANEALTP 118
M14_ASTE_ASPA-like cd06256
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 45-147 1.75e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349474  Cd Length: 204  Bit Score: 41.90  E-value: 1.75e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  45 GPHVMINALTHGNELCGAIAVDALLAAGVRPARGRLTLSFANVEAYERfdindpdATRFID--EDLNRVWSadkldGPGN 122
Cdd:cd06256    34 PRPLFVSTLLHGNEPTGLRAVQRLLKTGQAPLPRTLLLFIGNVDAAKA-------GVRRLPgqPDYNRCWP-----GPFE 101

                          90       100
                  ....*....|....*....|....*..
gi 1079661938 123 SLELRRARELRPVIDT--VDYLLDIHS 147
Cdd:cd06256   102 TPEGRLAAAVLERLDTlrPFASIDIHN 128
M14_ASTE_ASPA-like cd06255
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 44-234 2.56e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349473  Cd Length: 223  Bit Score: 41.54  E-value: 2.56e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  44 PGPHVMINALTHGNELCGAIAVDALLaAGVRPARGRLTLSF---AN---VEAYERFdindpdaTRFIDEDLNRVWSADKl 117
Cdd:cd06255    22 PGPCLWINGAVHGDELNGPLAALELF-RELDPAQLSGTLVAtpiANplaFQGRQKF-------SPQDGEDLDQSFPGDP- 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 118 DGPGNSlelRRA----RELRPVidtVDYLLDIHSMH----EKAEPLMLTGPHAKGVA------LGRLVGAPSHLMIDAGH 183
Cdd:cd06255    93 DGLITE---RMAhalfSEVKEV---ADYLIDFHTGGtpfdANPYTVYKLFPESGPVEekrllrLARAFGVHANCRVDVSG 166

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1079661938 184 AAGRRLRDYGGFGD---AASPKNALLIECG--QHFERVSRDVALDACARFLVALGT 234
Cdd:cd06255   167 AGGELPGNTAGALDyqcMAQGIPAFMVELGggGRAEEEAVRFAARGLRNLLRYLGM 222
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 50-222 4.50e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 40.69  E-value: 4.50e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  50 INALTHGNELCGAIAVDALlAAGVRPA--RGRLTLS-FANVEAYER--FDINDPDatrfiDEDLNRVWSADKlDGpgnSL 124
Cdd:cd18174     3 VTAGVHGYEYASIEALQRL-IKELDPAklSGTVIVVpIANIPAFEGrsIYVNPLD-----GKNLNRSFPGDP-DG---TP 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 125 ELRRAREL-RPVIDTVDYLLDIHS--MHEKAEP--LMLTGPHAKGVALGRLV----GAPsHLMIDAGHAAGRRLRDYGGF 195
Cdd:cd18174    73 TERLAHWLtTNVIARADYYIDLHGgdLNEDLRPfvYYYETGNAALDAASREMaeafGLD-HIVFYKARLKASRGSLYTQA 151

                         170       180
                  ....*....|....*....|....*..
gi 1079661938 196 GDAASPKNALLIECGQHFERVSRDVAL 222
Cdd:cd18174   152 AALLRGIPAILVEAGGLGSRDEEDVAR 178
M14_ASTE_ASPA-like cd06252
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 44-186 6.92e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349470  Cd Length: 224  Bit Score: 40.25  E-value: 6.92e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938  44 PGPHVMINALTHGNELCGAIAVdALLAAGVRPA--RGRLT-LSFANVEAYErfdindpDATRF--IDE-DLNRVWSADKL 117
Cdd:cd06252    33 SGPTVLLTGGNHGDEYEGPIAL-RRLARDLDPEdvRGRLIiVPALNLPAVR-------AGTRTspLDGgNLNRAFPGDAD 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079661938 118 DGPGNSLELRRARELRPVidtVDYLLDIHS------------MHEKAEPLMltgpHAKGVALGRLVGAPSHLMIDAGHAA 185
Cdd:cd06252   105 GTPTERIAHFLETVLLPR---ADAVIDLHSggssldfvpcaaVHLLPDPAQ----RARSLALAEAFGAPLSVVVDNVDAP 177


                  .
gi 1079661938 186 G 186
Cdd:cd06252   178 G 178
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH